AT2G45000

Nsp1-like C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013006
Physical Location & Seq
Forward (+)
18564017 .. 18567892
3876 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45000.1

Sequence Viewer

Length: 2220 bp
ATGTCGGGGTTTCCATTTGGTCAATCCAATTCCGTTGGAGGATTCTCATTCGGATCTTCTTCTGCAACTAATTCCTCTTCTGCTTCATCTACCACTTCTCCGTTATCTTTCTCCTTTAACCAATCTTCAAACCCTAGCTCCACCGGATTCGGTTTCGGATCCTCCGTATCATCAACTCCTGCTTCCTCCACTACTCCATCGTTTGGATTCGGAGCTTCCTCTACTCCTTCCTTCGGATTCGGTTCTTCTGCTTCTTCATCGACTCCATCGTTCGGATTCGGATCTTCTGCCTCCGTGACTCCTGCTTCCACAACTCCATCGTTTGGATTTGGAACCGCCGCTTCTTCATCTGCTCCTGCTCCGTCGCTGTTTGGTTCATCTACCACCAATGCTTCTTCAGCTGCTCCTGGTTCTTCTCCTTTCGGATTTGTAACTTCCTCGGCCTCTTCCACCGCAACTCCATCGTCATCTCTATTTGGAGCTCCCGCTTCTTCCGCCGCAACTCCATCGTCATCTCCATTCGGAGCTGCTCCAGCCAGTGGTTCAACACCTCTCTTTGGATCTTCCCCTTCCTTATTCTCTGCTCCTTCATCTGCCTCCGCCTCTAATTCTTCTCTCTTCGGAGCATCCTCATCCGCCGCTACTTCAACATCTCCTCTTTTCGGCGCGCCATCATCAGCCACCGGCGCAACACCGTCCTTTAGCGTGGCCTCATCAGCACCTGGCTCCTCATCATCCATCTTTGGAGCTACCGGCTCGTCACCTTCCTTCAGTGTTGCCTCATCAGCCTCTGGCTCCTCACCGTCAATCTTTGGAGCTACCGGTTCTTCACCGTTCTTTGGTTCGTCTTCTTCAGCCGGTTCAACTCCGTCTCTCTTTGCATCTTCCTCGTCAGGCGCAACAACTTCTTCACCATCCCCGTTTGGAGTATCCACCTTCAATTCCTCATCTACATCCAATACCTCGAATGCCTCAGCTTCTCCATTCTCCGCTTCTACCGGGTTCTCTTTCTTGAAAAGCACTGCAAGTTCGACCACCAGCTCCACCACTCCTTCTGCTCCTCCACAAACGGCTTCATCTTCGTCCTCCTTCTCATTTGGTACATCCGCCAATTCAGGTTTTAACCTTTCAACCGGAAGCTCCGCAGCACCTGCATCTAGCACATCCGGAGCAGTGTTTTCCATTGCAACCACCACTACTACTTCTTCTTCGACTCCGGCTGCTACTAGTGCACCAGCTTCTTCAGCTCCCGCTTCTACAATGGCTTTCCCTTCTTTTGGTGTGACTTCATCTGCAACCAACACCACTCCGGCTAGCTCCGCTGCTACATTCAGTACTACAGGATTTGGTTTGGCTAGCTCGACTCCTGCCACTGGATCTACGAACTCTTTTACTGGGTTTGCTGTACCGAAGACATCAACACCTGCATCATCTTCACAACCACAAACTACTAGTCCTGCATTCTCATTCAGTCTCCCATCTTCGACTTCTACTACTGCTCCTGCTACTAGTTCTGCTACTACAACACAGACAACTCTTGTTGTTCCTTCAAGTAGTGGGACGAGCACAGCTGTTGCTCCAGTGGCTGGTTCTCCAAAGTTGCCATCTGAAATAACTGGCAAAACTGTTGAGGAGATTATCAAGGAGTGGAATACTGAGCTACAAGAGCGCACAGGGAGATTCCGGAAACAGGCAAATGCAATAGCAGAGTGGGATAAGCGGATCTTGCAAAACCGTGATGTTCTTTTGAGACTTGAGATTGAAGTTGCAAAAGTGGTTGAAACCCAATCTAGCTTGGAACGACAGCTGGAATTAATCGAGACTCATCAGCAAGAGGTTGACAAGGCTTTGCAAAGCATGGAGGAAGAGGCTGAGCGTATATATAATGACGAGCGGAAATCACTTCTGGACGATGAAGCAGCATCAACTAGAGATGCAATGTATGAACAGTCTGAGCTAGTAGAAAGAGAACTGGAGCATATGACTGAACAAATCAGATCGATTATACAATCCGTAAATGCAAACCAGGGTGGAGAACTTGAAGCGATAGATGGGATGAGTCCACTTGATGTAGTTGTCAGAATCTTGAACAATCAACTCAGTTCCTTAATGTGGATTGATGAGAAAGCAGAGGAATTCTCTTCTCGGATTCAAAAAATCGCATTGCAAGGTTCGGGTGGAGATCGTGAATTGATGGCTCCAAAGCACTGGATGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0003674 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005642 GO:0005643 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0008092 GO:0008104 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008285 GO:0008289 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010648 GO:0010928 GO:0010930 GO:0010941 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016043 GO:0016070 GO:0017038 GO:0017056 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019894 GO:0019904 GO:0019953 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030159 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032182 GO:0032386 GO:0032501 GO:0032504 GO:0032774 GO:0032879 GO:0032880 GO:0032947 GO:0032991 GO:0033036 GO:0033157 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0042127 GO:0042169 GO:0042175 GO:0042306 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043123 GO:0043130 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044613 GO:0044703 GO:0045184 GO:0045893 GO:0045935 GO:0046483 GO:0046822 GO:0046907 GO:0046966 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051223 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051259 GO:0051291 GO:0051427 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0060255 GO:0060341 GO:0060548 GO:0065003 GO:0065007 GO:0070013 GO:0070201 GO:0070206 GO:0070208 GO:0070727 GO:0071166 GO:0071426 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0098589 GO:0098805 GO:1900180 GO:1901360 GO:1901362 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1903827 GO:1904589 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nsp1_C Nucleoporin_FG

Protein Analysis

739

Amino Acids

73.48

Weight (kDa)

4.77

Isoelectric Point (pI)

65.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nsp1_C PF05064 534 - 633 8.1e-22 Nsp1-like C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45000
fragaria_vesca FvH4_7g10470 FvH4_7g10470 FvH4_7g10470
malus_domestica MD07G1110700.v1.1
prunus_persica Prupe.2G134200_v2.0.a1 Prupe.2G134200_v2.0.a1
pyrus_communis pycom07g09560
rosa_chinensis RchiOBHm_Chr1g0348261
rosa_laevigata RLG00000028672 RLG00000028676
rosa_roxburghii Rroxscaffold_4G00307070
rosa_rugosa Rorug01G0194000
rosa_samantha Rh1AG211000 Rh1BG177500 Rh1CG195400 Rh1DG207000
rosa_wichuraiana Rw1G017820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1159, 1432
Acc36I ACCTGC 2 cut(s) 1159, 1432
AccB7I CCANNNNNTGG 4 cut(s) 203, 323, 539, 1586
AccBSI CCGCTC 1 cut(s) 1894
AccII CGCG 1 cut(s) 668
AccIII TCCGGA 2 cut(s) 1166, 1683
AclWI GGATC 7 cut(s) 61, 153, 166, 289, 568, 1384, 1730
AcsI RAATTY 1 cut(s) 2135
AcuI CTGAAG 4 cut(s) 381, 754, 837, 1227
AfaI GTAC 3 cut(s) 1102, 1336, 1407
AgeI ACCGGT 1 cut(s) 821
AhlI ACTAGT 3 cut(s) 1226, 1451, 1508
AjnI CCWGG 3 cut(s) 406, 721, 2025
Alw21I GWGCWC 3 cut(s) 484, 1234, 1568
Alw26I GTCTC 4 cut(s) 876, 1478, 1744, 1814
Alw44I GTGCAC 1 cut(s) 1230
AlwI GGATC 7 cut(s) 61, 153, 166, 289, 568, 1384, 1730
AlwNI CAGNNNCTG 4 cut(s) 722, 791, 1151, 1586
Aor13HI TCCGGA 2 cut(s) 1166, 1683
AoxI GGCC 2 cut(s) 441, 708
ApaLI GTGCAC 1 cut(s) 1230
ApeKI GCWGC 6 cut(s) 401, 527, 1145, 1220, 1322, 1919
ApoI RAATTY 1 cut(s) 2135
ArsI GACNNNNNNTTYG 2 cut(s) 1438, 1470
AscI GGCGCGCC 1 cut(s) 666
AseI ATTAAT 1 cut(s) 1814
AsiGI ACCGGT 1 cut(s) 821
AspLEI GCGC 5 cut(s) 668, 670, 689, 899, 1671
AsuC2I CCSGG 1 cut(s) 1000
AsuHPI GGTGA 4 cut(s) 753, 792, 822, 903
AsuNHI GCTAGC 2 cut(s) 1313, 1355
BaeGI GKGCMC 1 cut(s) 1234
BamHI GGATCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 484
BbsI GAAGAC 2 cut(s) 840, 1418
Bbv12I GWGCWC 3 cut(s) 484, 1234, 1568
BbvCI CCTCAGC 1 cut(s) 973
BbvI GCAGC 6 cut(s) 388, 514, 1157, 1207, 1309, 1931
BceAI ACGGC 1 cut(s) 1086
BcgI CGANNNNNNTGC 6 cut(s) 444, 478, 489, 523, 870, 904
BciT130I CCWGG 3 cut(s) 408, 723, 2027
BciVI GTATCC 1 cut(s) 940
BcnI CCSGG 1 cut(s) 1000
BcoDI GTCTC 4 cut(s) 876, 1478, 1744, 1814
BcuI ACTAGT 3 cut(s) 1226, 1451, 1508
BfmI CTRYAG 1 cut(s) 1338
BfuAI ACCTGC 2 cut(s) 1159, 1432
BfuI GTATCC 1 cut(s) 940
BisI GCNGC 9 cut(s) 339, 402, 498, 528, 639, 1146, 1221, 1323, 1920
BlpI GCTNAGC 1 cut(s) 1872
BlsI GCNGC 9 cut(s) 340, 403, 499, 529, 640, 1147, 1222, 1324, 1921
BmcAI AGTACT 1 cut(s) 1336
Bme1390I CCNGG 4 cut(s) 408, 723, 1000, 2027
BmiI GGNNCC 5 cut(s) 160, 334, 727, 796, 2199
BmrFI CCNGG 4 cut(s) 408, 723, 1000, 2027
BmrI ACTGGG 1 cut(s) 1404
BmsI GCATC 6 cut(s) 635, 890, 1163, 1436, 1924, 1931
BmtI GCTAGC 2 cut(s) 1317, 1359
BmuI ACTGGG 1 cut(s) 1404
BpiI GAAGAC 2 cut(s) 840, 1418
BplI GAGNNNNNCTC 2 cut(s) 2123, 2155
BpmI CTGGAG 3 cut(s) 516, 1563, 1994
Bpu10I CCTNAGC 1 cut(s) 973
Bpu1102I GCTNAGC 1 cut(s) 1872
BpuEI CTTGAG 1 cut(s) 1775
BpuMI CCSGG 1 cut(s) 1000
Bsa29I ATCGAT 1 cut(s) 2000
BsaBI GATNNNNATC 1 cut(s) 280
BsaJI CCNNGG 2 cut(s) 438, 2026
BsaWI WCCGGW 5 cut(s) 143, 821, 1133, 1166, 1683
BsaXI ACNNNNNCTCC 8 cut(s) 82, 112, 122, 152, 442, 472, 1483, 1513
Bse118I RCCGGY 4 cut(s) 683, 752, 821, 857
Bse1I ACTGG 7 cut(s) 537, 1378, 1399, 1580, 1621, 1977, 2213
Bse3DI GCAATG 3 cut(s) 1182, 1944, 2162
Bse8I GATNNNNATC 1 cut(s) 280
BseAI TCCGGA 2 cut(s) 1166, 1683
BseBI CCWGG 3 cut(s) 408, 723, 2027
BseCI ATCGAT 1 cut(s) 2000
BseDI CCNNGG 2 cut(s) 438, 2026
BseGI GGATG 9 cut(s) 626, 632, 734, 914, 953, 1103, 1163, 2061, 2217
BseJI GATNNNNATC 1 cut(s) 280
BseMI GCAATG 3 cut(s) 1182, 1944, 2162
BseMII CTCAG 5 cut(s) 987, 1647, 1863, 1944, 2113
BseNI ACTGG 7 cut(s) 537, 1378, 1399, 1580, 1621, 1977, 2213
BsePI GCGCGC 1 cut(s) 666
BseRI GAGGAG 5 cut(s) 645, 718, 787, 1050, 1646
BseSI GKGCMC 1 cut(s) 1234
BseXI GCAGC 6 cut(s) 388, 514, 1157, 1207, 1309, 1931
Bsh1236I CGCG 1 cut(s) 668
BshFI GGCC 2 cut(s) 443, 710
BshTI ACCGGT 1 cut(s) 821
BshVI ATCGAT 1 cut(s) 2000
BsiHKAI GWGCWC 3 cut(s) 484, 1234, 1568
BslFI GGGAC 1 cut(s) 1573
BsmAI GTCTC 4 cut(s) 876, 1478, 1744, 1814
BsmBI CGTCTC 1 cut(s) 876
BsmFI GGGAC 1 cut(s) 1573
BsmI GAATGC 2 cut(s) 973, 1460
BsnI GGCC 2 cut(s) 443, 710
Bsp1286I GDGCHC 3 cut(s) 484, 1234, 1568
Bsp13I TCCGGA 2 cut(s) 1166, 1683
Bsp143I GATC 8 cut(s) 53, 158, 281, 560, 1376, 1722, 1997, 2182
Bsp1720I GCTNAGC 1 cut(s) 1872
BspANI GGCC 2 cut(s) 443, 710
BspCNI CTCAG 5 cut(s) 986, 1648, 1864, 1945, 2112
BspDI ATCGAT 1 cut(s) 2000
BspEI TCCGGA 2 cut(s) 1166, 1683
BspFNI CGCG 1 cut(s) 668
BspLI GGNNCC 5 cut(s) 160, 334, 727, 796, 2199
BspMI ACCTGC 2 cut(s) 1159, 1432
BspOI GCTAGC 2 cut(s) 1317, 1359
BspPI GGATC 7 cut(s) 61, 153, 166, 289, 568, 1384, 1730
BsrBI CCGCTC 1 cut(s) 1894
BsrDI GCAATG 3 cut(s) 1182, 1944, 2162
BsrFI RCCGGY 4 cut(s) 683, 752, 821, 857
BsrI ACTGG 7 cut(s) 537, 1378, 1399, 1580, 1621, 1977, 2213
BssAI RCCGGY 4 cut(s) 683, 752, 821, 857
BssECI CCNNGG 2 cut(s) 438, 2026
BssHII GCGCGC 1 cut(s) 666
BssMI GATC 8 cut(s) 53, 158, 281, 560, 1376, 1722, 1997, 2182
Bst2UI CCWGG 3 cut(s) 408, 723, 2027
Bst4CI ACNGT 6 cut(s) 696, 804, 834, 1627, 1736, 1950
Bst6I CTCTTC 5 cut(s) 82, 451, 623, 1860, 2146
BstAPI GCANNNNNTGC 1 cut(s) 1151
BstC8I GCNNGC 3 cut(s) 668, 1315, 1357
BstDEI CTNAG 5 cut(s) 973, 1656, 1872, 1953, 2099
BstF5I GGATG 9 cut(s) 626, 632, 734, 914, 953, 1103, 1163, 2061, 2217
BstFNI CGCG 1 cut(s) 668
BstHHI GCGC 5 cut(s) 668, 670, 689, 899, 1671
BstKTI GATC 8 cut(s) 56, 161, 284, 563, 1379, 1725, 2000, 2185
BstMAI GTCTC 4 cut(s) 876, 1478, 1744, 1814
BstMBI GATC 8 cut(s) 53, 158, 281, 560, 1376, 1722, 1997, 2182
BstNI CCWGG 3 cut(s) 408, 723, 2027
BstSCI CCNGG 4 cut(s) 406, 721, 998, 2025
BstSFI CTRYAG 1 cut(s) 1338
BstSLI GKGCMC 1 cut(s) 1234
BstUI CGCG 1 cut(s) 668
BstV1I GCAGC 6 cut(s) 388, 514, 1157, 1207, 1309, 1931
BstV2I GAAGAC 2 cut(s) 840, 1418
BstX2I RGATCY 6 cut(s) 53, 158, 281, 560, 1376, 1722
BstXI CCANNNNNNTGG 1 cut(s) 2208
BstYI RGATCY 6 cut(s) 53, 158, 281, 560, 1376, 1722
Bsu15I ATCGAT 1 cut(s) 2000
BsuI GTATCC 1 cut(s) 940
BsuRI GGCC 2 cut(s) 443, 710
BsuTUI ATCGAT 1 cut(s) 2000
BtsCI GGATG 9 cut(s) 626, 632, 734, 914, 953, 1103, 1163, 2061, 2217
BtsI GCAGTG 2 cut(s) 1020, 1179
BtsIMutI CAGTG 7 cut(s) 544, 778, 1020, 1179, 1371, 1587, 2206
BveI ACCTGC 2 cut(s) 1159, 1432
Cac8I GCNNGC 3 cut(s) 668, 1315, 1357
CaiI CAGNNNCTG 4 cut(s) 722, 791, 1151, 1586
CfoI GCGC 5 cut(s) 668, 670, 689, 899, 1671
Cfr10I RCCGGY 4 cut(s) 683, 752, 821, 857
ClaI ATCGAT 1 cut(s) 2000
Csp6I GTAC 3 cut(s) 1101, 1335, 1406
CspAI ACCGGT 1 cut(s) 821
CviAII CATG 1 cut(s) 1858
CviQI GTAC 3 cut(s) 1101, 1335, 1406
DdeI CTNAG 5 cut(s) 973, 1656, 1872, 1953, 2099
DpnI GATC 8 cut(s) 55, 160, 283, 562, 1378, 1724, 1999, 2184
DpnII GATC 8 cut(s) 53, 158, 281, 560, 1376, 1722, 1997, 2182
Eam1104I CTCTTC 5 cut(s) 82, 451, 623, 1860, 2146
EarI CTCTTC 5 cut(s) 82, 451, 623, 1860, 2146
EciI GGCGGA 4 cut(s) 484, 589, 625, 1096
Ecl136II GAGCTC 1 cut(s) 482
Eco24I GRGCYC 1 cut(s) 484
Eco53kI GAGCTC 1 cut(s) 482
Eco57I CTGAAG 4 cut(s) 381, 754, 837, 1227
EcoICRI GAGCTC 1 cut(s) 482
EcoRI GAATTC 1 cut(s) 2135
EcoRII CCWGG 3 cut(s) 406, 721, 2025
EcoT38I GRGCYC 1 cut(s) 484
Esp3I CGTCTC 1 cut(s) 876
FaeI CATG 1 cut(s) 1861
FaiI YATR 8 cut(s) 1859, 1880, 1882, 1884, 1944, 1980, 1982, 2006
FalI AAGNNNNNCTT 2 cut(s) 1709, 1741
FaqI GGGAC 1 cut(s) 1573
FatI CATG 1 cut(s) 1857
FauI CCCGC 2 cut(s) 493, 1258
FauNDI CATATG 1 cut(s) 1980
Fnu4HI GCNGC 9 cut(s) 339, 402, 498, 528, 639, 1146, 1221, 1323, 1920
FokI GGATG 8 cut(s) 613, 619, 721, 901, 940, 1090, 1150, 2068
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 9 cut(s) 339, 402, 498, 528, 639, 1146, 1221, 1323, 1920
GlaI GCGC 5 cut(s) 667, 669, 688, 898, 1670
GluI GCNGC 9 cut(s) 339, 402, 498, 528, 639, 1146, 1221, 1323, 1920
GsuI CTGGAG 3 cut(s) 516, 1563, 1994
HaeIII GGCC 2 cut(s) 443, 710
HhaI GCGC 5 cut(s) 668, 670, 689, 899, 1671
Hin1II CATG 1 cut(s) 1861
Hin6I GCGC 5 cut(s) 666, 668, 687, 897, 1669
HinP1I GCGC 5 cut(s) 666, 668, 687, 897, 1669
HincII GTYRAC 1 cut(s) 1840
HindII GTYRAC 1 cut(s) 1840
HphI GGTGA 4 cut(s) 753, 792, 822, 903
Hpy166II GTNNAC 3 cut(s) 1232, 1840, 2063
Hpy188III TCNNGA 8 cut(s) 1012, 1167, 1684, 1819, 1907, 2086, 2186, 2217
Hpy8I GTNNAC 3 cut(s) 1232, 1840, 2063
Hpy99I CGWCG 1 cut(s) 367
HpyCH4III ACNGT 6 cut(s) 696, 804, 834, 1627, 1736, 1950
HpyF3I CTNAG 5 cut(s) 973, 1656, 1872, 1953, 2099
Hsp92II CATG 1 cut(s) 1861
HspAI GCGC 5 cut(s) 666, 668, 687, 897, 1669
Kpn2I TCCGGA 2 cut(s) 1166, 1683
Kzo9I GATC 8 cut(s) 53, 158, 281, 560, 1376, 1722, 1997, 2182
Lsp1109I GCAGC 6 cut(s) 388, 514, 1157, 1207, 1309, 1931
LweI GCATC 6 cut(s) 635, 890, 1163, 1436, 1924, 1931
MaeIII GTNAC 4 cut(s) 295, 430, 759, 1282
MalI GATC 8 cut(s) 55, 160, 283, 562, 1378, 1724, 1999, 2184
MbiI CCGCTC 1 cut(s) 1894
MboI GATC 8 cut(s) 53, 158, 281, 560, 1376, 1722, 1997, 2182
MflI RGATCY 6 cut(s) 53, 158, 281, 560, 1376, 1722
MhlI GDGCHC 3 cut(s) 484, 1234, 1568
MluCI AATT 8 cut(s) 28, 70, 607, 940, 1111, 1811, 2135, 2189
MlyI GAGTC 6 cut(s) 256, 292, 1207, 1357, 1816, 2068
MmeI TCCRAC 1 cut(s) 16
MroI TCCGGA 2 cut(s) 1166, 1683
MseI TTAA 4 cut(s) 117, 1122, 1814, 2108
MspA1I CMGCKG 4 cut(s) 401, 1322, 1571, 1807
MspR9I CCNGG 4 cut(s) 408, 723, 1000, 2027
Mva1269I GAATGC 2 cut(s) 973, 1460
MvaI CCWGG 3 cut(s) 408, 723, 2027
MvnI CGCG 1 cut(s) 668
NciI CCSGG 1 cut(s) 1000
NdeI CATATG 1 cut(s) 1980
NdeII GATC 8 cut(s) 53, 158, 281, 560, 1376, 1722, 1997, 2182
NheI GCTAGC 2 cut(s) 1313, 1355
NlaIII CATG 1 cut(s) 1861
NlaIV GGNNCC 5 cut(s) 160, 334, 727, 796, 2199
NmeAIII GCCGAG 1 cut(s) 419
NmuCI GTSAC 3 cut(s) 295, 759, 1282
PalAI GGCGCGCC 1 cut(s) 666
PaqCI CACCTGC 2 cut(s) 1159, 1432
PauI GCGCGC 1 cut(s) 666
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 266
PctI GAATGC 2 cut(s) 973, 1460
PfeI GAWTC 8 cut(s) 42, 147, 207, 237, 276, 1680, 2082, 2149
PflMI CCANNNNNTGG 4 cut(s) 203, 323, 539, 1586
PinAI ACCGGT 1 cut(s) 821
PkrI GCNGC 9 cut(s) 340, 403, 499, 529, 640, 1147, 1222, 1324, 1921
PleI GAGTC 6 cut(s) 256, 292, 1207, 1357, 1816, 2067
PpsI GAGTC 6 cut(s) 256, 292, 1207, 1357, 1816, 2067
PshBI ATTAAT 1 cut(s) 1814
Psp124BI GAGCTC 1 cut(s) 484
Psp6I CCWGG 3 cut(s) 406, 721, 2025
PspGI CCWGG 3 cut(s) 406, 721, 2025
PspN4I GGNNCC 5 cut(s) 160, 334, 727, 796, 2199
PstNI CAGNNNCTG 4 cut(s) 722, 791, 1151, 1586
PsuI RGATCY 6 cut(s) 53, 158, 281, 560, 1376, 1722
PteI GCGCGC 1 cut(s) 666
PvuII CAGCTG 3 cut(s) 401, 1571, 1807
RsaI GTAC 3 cut(s) 1102, 1336, 1407
RsaNI GTAC 3 cut(s) 1101, 1335, 1406
SacI GAGCTC 1 cut(s) 484
SaqAI TTAA 4 cut(s) 117, 1122, 1814, 2108
SatI GCNGC 9 cut(s) 339, 402, 498, 528, 639, 1146, 1221, 1323, 1920
Sau3AI GATC 8 cut(s) 53, 158, 281, 560, 1376, 1722, 1997, 2182
ScaI AGTACT 1 cut(s) 1336
SchI GAGTC 6 cut(s) 256, 292, 1207, 1357, 1816, 2068
ScrFI CCNGG 4 cut(s) 408, 723, 1000, 2027
SduI GDGCHC 3 cut(s) 484, 1234, 1568
SfaNI GCATC 6 cut(s) 635, 890, 1163, 1436, 1924, 1931
SfcI CTRYAG 1 cut(s) 1338
SgrAI CRCCGGYG 1 cut(s) 683
SgsI GGCGCGCC 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 1754
SmoI CTYRAG 1 cut(s) 1754
SpeI ACTAGT 3 cut(s) 1226, 1451, 1508
Sse9I AATT 8 cut(s) 28, 70, 607, 940, 1111, 1811, 2135, 2189
SstI GAGCTC 1 cut(s) 484
StyD4I CCNGG 4 cut(s) 406, 721, 998, 2025
TaaI ACNGT 6 cut(s) 696, 804, 834, 1627, 1736, 1950
TaqI TCGA 8 cut(s) 260, 965, 1031, 1211, 1361, 1484, 1818, 2000
TasI AATT 8 cut(s) 28, 70, 607, 940, 1111, 1811, 2135, 2189
TatI WGTACW 1 cut(s) 1334
TauI GCSGC 3 cut(s) 341, 500, 641
TfiI GAWTC 8 cut(s) 42, 147, 207, 237, 276, 1680, 2082, 2149
Tru1I TTAA 4 cut(s) 117, 1122, 1814, 2108
Tru9I TTAA 4 cut(s) 117, 1122, 1814, 2108
TscAI CASTG 7 cut(s) 544, 778, 1027, 1179, 1378, 1587, 2213
TseFI GTSAC 3 cut(s) 295, 759, 1282
TseI GCWGC 6 cut(s) 401, 527, 1145, 1220, 1322, 1919
Tsp45I GTSAC 3 cut(s) 295, 759, 1282
TspDTI ATGAA 9 cut(s) 75, 246, 336, 366, 579, 1065, 1278, 1929, 1959
TspGWI ACGGA 7 cut(s) 22, 90, 154, 283, 351, 858, 2002
TspRI CASTG 7 cut(s) 544, 778, 1027, 1179, 1378, 1587, 2213
Van91I CCANNNNNTGG 4 cut(s) 203, 323, 539, 1586
VneI GTGCAC 1 cut(s) 1230
VspI ATTAAT 1 cut(s) 1814
XapI RAATTY 1 cut(s) 2135
ZrmI AGTACT 1 cut(s) 1336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.