MD07G1110700.v1.1

Nsp1-like C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013006
Physical Location & Seq
Forward (+)
12755345 .. 12762516
7172 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1110700.v1.1.491

Sequence Viewer

Length: 2172 bp
ATGTCGGGATTTTCATCCGGATCCTCCGCCTCCTCCTCTTCCGCCCAATCCACTTCCCCTTTCTCTTTCTCCAACCCTCCTTCCTCCTTCTCATTCGGCCCTTCCGCCTCCCCATTCGGATCCTCTACACCTGCCACCACGTCCACCACCGGCTTCTCCATTGGATCCTCGGCCTTCGGCGCCTCCACTCCCTCTAACCCTTCCTCCTCCCCATCTCCATTCTCCTTCTCCCTCAACTCCACTTCCACTCCCTCCTCCTCCCTATTCGGATCCTCAAACCCTAGCTCCGCCACTACCCCTTCCTTCGGTTTCGCACCCACCACCACCACAACAACGTCTTCGGCTTCCGCCCCTTCTTTCGGATTCATACCCTCCTCTACCAACACCACAACCTCATCTGCCTCCGCTTCGTTTGGATTCTCATCCGCCGCCTCCGCTGCCGCCACCACCACAACCTCCTCTGCTTCCACTCCTTCTTTTGGATTCTCATCAGCCGCATCCTCTGGCCTTTCCCTGTTTGGCTCCTCGGCCTCAACGGCTTCAACTCCTCTGTTTGGTTCAACTCCGTCCCAATCCCTGTTTGGCTCGTCCTCATCGGCTCCCACCTCCACCGCCTCCGTCTCCCCTCTATTCGGAGCTCCTTCTTCCGCCACTTCATCCCCATTTGGAGCAACCTCATTTGCACCCAGCGCACCCACACCCCTGTTTGGAACCTCCTCCACTTCCGCTTCGCAGCTGTTTGGCACAGCGTCCTCTGCACCCAGCGCACCCTCGCCGCTGTTTGGCACAGCTTCCTCGTCCCAACCTTCATCCTCTGTAGCTCCAACGACCCAATCGTTCCAAAGCTTCTCCACTTCCTCTTCAGCCTTCACATTACCAACATCCTCCCCTCTTTTGAAACCCACAACCTCCACTCCTACCACCGCTGCTCCTCCATCAACAACCGCTTCCAGCTTCTCCTCCTTCGGAACGCCGGCTTCCTCCGCTTCACAATCCTCTTTCGGTTTCTCCACCAGTTTCTCCAATGCCGCCTCAAAACCAACCTCACAATCCTTCCCAACTCCCTCCGCTCCGTTTCTTTCTGCTCTCACGACCACAACCCCTTCTTCGACTCCAGCAACTAGCACTACAACCCAAGCCTCTTTCACTATGCCGTCTTTTGCTGTGAATCCTGCTACTTCTCAGGCTCCTAGCACCGCTGCAACAACGGCTCCAATGGCGGCCCCTGCGAGCTCGGCGGCCCCTGCGAGCTCAGCAGCTTCTTCCGGGGGCTCGCTTGTAAGCTCGTTCTCCTTCCAAACTGCTTCAGGAGCTACTGCTTCTTCTTCTGCTGGCTTTTTGTCTTTGACAAAAGGGTCTACACCAGCTCCATCGTCTCAAGCACAACCAAGCACTACCGTGCCTGCATTGGGTGTCGGTACTTCAGCCCCTGTAACTGCTACGACAACCACTACAAGTACTGCTGCAACTCAGACATCAACATCACTTGTTGCAGCTTCTACTAGTGGGACAACTTCAAATGTCAACACTACAGTATCTACTGCACCAAAATTACCGTCTGAAATCACAGGGAAGACTGTGGAGGAGATCATCAAGGAGTGGAATGCTGAGCTTCAAGAGCGTACTGGAAAATTCCGAAAGCAGGCTAATGCAATAGCAGATTGGGACAAAAGGATTTTGCACAACCGTGATGTTCTTCTTAGGCTCGAGATTGAAGTGGCGAAAGTGGTTGAAACCCAAGCTAACTTGGAGCGGCAACTAGAGTTAATTGAGACTCATCAGCAAGAGGTTGATAAGGCTTTGCAAAGCATGGAAGAAGAAGCTGAGCGTATATACAAGGATGAACGTGGATTGCTCCGTGATGATGAGGCTGCATCCACAAGAGATGCAATGTATGAGCAAGCTGAGTTTGTAGAGAGGGAACTAGAGCAGGTGACAGAACAGATCAAATCAATTATTCAGACCTTAAATGCCAACCAGGGAGGGGAACTTGATACAAATGATGGTATGGCTCCGTTAGATGTGGTTGTCCGAATCTTAAACAATCAGCTTAGCTCTTTAATGTGGATTGATGAAAAGGCCGAAGAATTCTCCTCGCGAATTCAGAAGCTCGCCAATGAAGGTCCTGCTGCAGATCGCGAATTAATGGCTCCAAAATACTGGATGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0003674 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005642 GO:0005643 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0008092 GO:0008104 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008285 GO:0008289 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010648 GO:0010928 GO:0010930 GO:0010941 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016043 GO:0016070 GO:0017038 GO:0017056 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019894 GO:0019904 GO:0019953 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030159 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032182 GO:0032386 GO:0032501 GO:0032504 GO:0032774 GO:0032879 GO:0032880 GO:0032947 GO:0032991 GO:0033036 GO:0033157 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0042127 GO:0042169 GO:0042175 GO:0042306 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043123 GO:0043130 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044613 GO:0044703 GO:0045184 GO:0045893 GO:0045935 GO:0046483 GO:0046822 GO:0046907 GO:0046966 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051223 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051259 GO:0051291 GO:0051427 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0060255 GO:0060341 GO:0060548 GO:0065003 GO:0065007 GO:0070013 GO:0070201 GO:0070206 GO:0070208 GO:0070727 GO:0071166 GO:0071426 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0098589 GO:0098805 GO:1900180 GO:1901360 GO:1901362 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1903827 GO:1904589 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nsp1_C Nucleoporin_FG

Protein Analysis

724

Amino Acids

72.92

Weight (kDa)

4.77

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nsp1_C PF05064 517 - 614 6.5e-21 Nsp1-like C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45000
fragaria_vesca FvH4_7g10470 FvH4_7g10470 FvH4_7g10470
malus_domestica MD07G1110700.v1.1
prunus_persica Prupe.2G134200_v2.0.a1 Prupe.2G134200_v2.0.a1
pyrus_communis pycom07g09560
rosa_chinensis RchiOBHm_Chr1g0348261
rosa_laevigata RLG00000028672 RLG00000028676
rosa_roxburghii Rroxscaffold_4G00307070
rosa_rugosa Rorug01G0194000
rosa_samantha Rh1AG211000 Rh1BG177500 Rh1CG195400 Rh1DG207000
rosa_wichuraiana Rw1G017820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 139, 1921
AasI GACNNNNNNGTC 1 cut(s) 1354
Acc36I ACCTGC 2 cut(s) 139, 1921
AccB1I GGYRCC 1 cut(s) 179
AccBSI CCGCTC 2 cut(s) 1070, 1753
AccI GTMKAC 1 cut(s) 1358
AccII CGCG 2 cut(s) 2098, 2139
AccIII TCCGGA 1 cut(s) 17
AclWI GGATC 8 cut(s) 15, 28, 114, 127, 159, 172, 264, 277
AcsI RAATTY 3 cut(s) 1631, 2087, 2100
AcuI CTGAAG 3 cut(s) 846, 1290, 1407
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 3 cut(s) 1420, 1459, 1624
AgsI TTSAA 7 cut(s) 543, 561, 898, 1518, 1616, 1715, 1733
AhlI ACTAGT 1 cut(s) 1502
AjiI CACGTC 1 cut(s) 141
AjnI CCWGG 1 cut(s) 1977
AleI CACNNNNGTG 2 cut(s) 1397, 1686
Alw21I GWGCWC 3 cut(s) 640, 1235, 1253
Alw26I GTCTC 3 cut(s) 625, 1380, 1766
AlwI GGATC 8 cut(s) 15, 28, 114, 127, 159, 172, 264, 277
AlwNI CAGNNNCTG 1 cut(s) 1430
Ama87I CYCGRG 1 cut(s) 1706
Aor13HI TCCGGA 1 cut(s) 17
AoxI GGCC 7 cut(s) 97, 171, 505, 528, 1221, 1239, 2079
ApeKI GCWGC 9 cut(s) 437, 733, 926, 1199, 1256, 1463, 1493, 1871, 2129
ApoI RAATTY 3 cut(s) 1631, 2087, 2100
ArsI GACNNNNNNTTYG 2 cut(s) 1541, 1573
AseI ATTAAT 1 cut(s) 2144
AspLEI GCGC 3 cut(s) 182, 692, 767
AspS9I GGNCC 4 cut(s) 98, 1222, 1240, 2123
AsuC2I CCSGG 1 cut(s) 1267
AsuHPI GGTGA 1 cut(s) 1945
AvaI CYCGRG 1 cut(s) 1706
AvaII GGWCC 1 cut(s) 2123
BaeI ACNNNNGTAYC 2 cut(s) 1518, 1551
BamHI GGATCC 4 cut(s) 20, 119, 164, 269
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 4 cut(s) 640, 1235, 1253, 1274
BbsI GAAGAC 2 cut(s) 330, 1580
Bbv12I GWGCWC 3 cut(s) 640, 1235, 1253
BbvI GCAGC 9 cut(s) 424, 745, 913, 1186, 1268, 1450, 1505, 1858, 2116
BccI CCATC 4 cut(s) 220, 943, 1378, 1997
BceAI ACGGC 3 cut(s) 552, 1138, 1224
BciT130I CCWGG 1 cut(s) 1979
BcnI CCSGG 1 cut(s) 1267
BcoDI GTCTC 3 cut(s) 625, 1380, 1766
BcuI ACTAGT 1 cut(s) 1502
BfaI CTAG 6 cut(s) 282, 1122, 1191, 1503, 1760, 1925
BfmI CTRYAG 3 cut(s) 816, 1530, 2130
BfoI RGCGCY 1 cut(s) 183
BfuAI ACCTGC 2 cut(s) 139, 1921
BglI GCCNNNNNGGC 1 cut(s) 536
BlpI GCTNAGC 4 cut(s) 1252, 1608, 1824, 2051
BmcAI AGTACT 1 cut(s) 1459
Bme1390I CCNGG 2 cut(s) 1267, 1979
Bme18I GGWCC 1 cut(s) 2123
BmeT110I CYCGRG 1 cut(s) 1706
BmgBI CACGTC 1 cut(s) 141
BmgT120I GGNCC 4 cut(s) 98, 1222, 1240, 2123
BmrFI CCNGG 2 cut(s) 1267, 1979
BmsI GCATC 3 cut(s) 506, 1876, 1883
BpiI GAAGAC 2 cut(s) 330, 1580
BpmI CTGGAG 1 cut(s) 1098
Bpu1102I GCTNAGC 4 cut(s) 1252, 1608, 1824, 2051
BpuEI CTTGAG 1 cut(s) 1362
BpuMI CCSGG 1 cut(s) 1267
BsaBI GATNNNNATC 3 cut(s) 13, 421, 487
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 4 cut(s) 168, 525, 1266, 1978
BsaWI WCCGGW 1 cut(s) 17
Bse118I RCCGGY 2 cut(s) 149, 973
Bse1I ACTGG 3 cut(s) 1014, 1630, 2165
Bse3DI GCAATG 1 cut(s) 1896
Bse8I GATNNNNATC 3 cut(s) 13, 421, 487
BseAI TCCGGA 1 cut(s) 17
BseBI CCWGG 1 cut(s) 1979
BseDI CCNNGG 4 cut(s) 168, 525, 1266, 1978
BseGI GGATG 9 cut(s) 14, 422, 497, 656, 809, 881, 1846, 1874, 2169
BseJI GATNNNNATC 3 cut(s) 13, 421, 487
BseMI GCAATG 1 cut(s) 1896
BseMII CTCAG 6 cut(s) 1196, 1266, 1484, 1599, 1815, 1896
BseNI ACTGG 3 cut(s) 1014, 1630, 2165
BseXI GCAGC 9 cut(s) 424, 745, 913, 1186, 1268, 1450, 1505, 1858, 2116
BseYI CCCAGC 2 cut(s) 686, 761
BsgI GTGCAG 2 cut(s) 741, 1527
Bsh1236I CGCG 2 cut(s) 2098, 2139
BshFI GGCC 7 cut(s) 99, 173, 507, 530, 1223, 1241, 2081
BshNI GGYRCC 1 cut(s) 179
BsiHKAI GWGCWC 3 cut(s) 640, 1235, 1253
BsiHKCI CYCGRG 1 cut(s) 1706
BsiSI CCGG 4 cut(s) 18, 150, 974, 1266
BslFI GGGAC 4 cut(s) 553, 784, 1522, 1679
BsmAI GTCTC 3 cut(s) 625, 1380, 1766
BsmBI CGTCTC 2 cut(s) 625, 1380
BsmFI GGGAC 4 cut(s) 553, 784, 1522, 1679
BsmI GAATGC 1 cut(s) 1609
BsnI GGCC 7 cut(s) 99, 173, 507, 530, 1223, 1241, 2081
BsoBI CYCGRG 1 cut(s) 1706
Bsp1286I GDGCHC 4 cut(s) 640, 1235, 1253, 1274
Bsp13I TCCGGA 1 cut(s) 17
Bsp143I GATC 7 cut(s) 20, 119, 164, 269, 1587, 1944, 2134
Bsp1720I GCTNAGC 4 cut(s) 1252, 1608, 1824, 2051
Bsp68I TCGCGA 2 cut(s) 2098, 2139
BspANI GGCC 7 cut(s) 99, 173, 507, 530, 1223, 1241, 2081
BspCNI CTCAG 6 cut(s) 1195, 1265, 1483, 1600, 1816, 1897
BspEI TCCGGA 1 cut(s) 17
BspFNI CGCG 2 cut(s) 2098, 2139
BspMAI CTGCAG 1 cut(s) 2134
BspMI ACCTGC 2 cut(s) 139, 1921
BspPI GGATC 8 cut(s) 15, 28, 114, 127, 159, 172, 264, 277
BspT107I GGYRCC 1 cut(s) 179
BsrBI CCGCTC 2 cut(s) 1070, 1753
BsrDI GCAATG 1 cut(s) 1896
BsrFI RCCGGY 2 cut(s) 149, 973
BsrI ACTGG 3 cut(s) 1014, 1630, 2165
BssAI RCCGGY 2 cut(s) 149, 973
BssECI CCNNGG 4 cut(s) 168, 525, 1266, 1978
BssMI GATC 7 cut(s) 20, 119, 164, 269, 1587, 1944, 2134
BssNI GRCGYC 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 1979
Bst4CI ACNGT 5 cut(s) 1399, 1534, 1557, 1579, 1688
Bst6I CTCTTC 2 cut(s) 43, 865
BstACI GRCGYC 1 cut(s) 180
BstC8I GCNNGC 9 cut(s) 975, 1231, 1249, 1274, 1333, 1404, 1644, 1902, 2112
BstDEI CTNAG 8 cut(s) 1182, 1252, 1470, 1608, 1700, 1824, 1905, 2051
BstF5I GGATG 9 cut(s) 14, 422, 497, 656, 809, 881, 1846, 1874, 2169
BstFNI CGCG 2 cut(s) 2098, 2139
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 3 cut(s) 182, 692, 767
BstKTI GATC 7 cut(s) 23, 122, 167, 272, 1590, 1947, 2137
BstMAI GTCTC 3 cut(s) 625, 1380, 1766
BstMBI GATC 7 cut(s) 20, 119, 164, 269, 1587, 1944, 2134
BstNI CCWGG 1 cut(s) 1979
BstSCI CCNGG 2 cut(s) 1265, 1977
BstSFI CTRYAG 3 cut(s) 816, 1530, 2130
BstUI CGCG 2 cut(s) 2098, 2139
BstV1I GCAGC 9 cut(s) 424, 745, 913, 1186, 1268, 1450, 1505, 1858, 2116
BstV2I GAAGAC 2 cut(s) 330, 1580
BstX2I RGATCY 4 cut(s) 20, 119, 164, 269
BstXI CCANNNNNNTGG 1 cut(s) 2160
BstYI RGATCY 4 cut(s) 20, 119, 164, 269
BsuRI GGCC 7 cut(s) 99, 173, 507, 530, 1223, 1241, 2081
BtrI CACGTC 1 cut(s) 141
BtsCI GGATG 9 cut(s) 14, 422, 497, 656, 809, 881, 1846, 1874, 2169
BtuMI TCGCGA 2 cut(s) 2098, 2139
BveI ACCTGC 2 cut(s) 139, 1921
Cac8I GCNNGC 9 cut(s) 975, 1231, 1249, 1274, 1333, 1404, 1644, 1902, 2112
CaiI CAGNNNCTG 1 cut(s) 1430
CfoI GCGC 3 cut(s) 182, 692, 767
Cfr10I RCCGGY 2 cut(s) 149, 973
Cfr13I GGNCC 4 cut(s) 98, 1222, 1240, 2123
CseI GACGC 1 cut(s) 738
Csp6I GTAC 3 cut(s) 1419, 1458, 1623
CviAII CATG 1 cut(s) 1810
CviQI GTAC 3 cut(s) 1419, 1458, 1623
DdeI CTNAG 8 cut(s) 1182, 1252, 1470, 1608, 1700, 1824, 1905, 2051
DinI GGCGCC 1 cut(s) 181
DpnI GATC 7 cut(s) 22, 121, 166, 271, 1589, 1946, 2136
DpnII GATC 7 cut(s) 20, 119, 164, 269, 1587, 1944, 2134
DrdI GACNNNNNNGTC 1 cut(s) 1354
DseDI GACNNNNNNGTC 1 cut(s) 1354
Eam1104I CTCTTC 2 cut(s) 43, 865
EarI CTCTTC 2 cut(s) 43, 865
EciI GGCGGA 7 cut(s) 16, 31, 94, 277, 337, 415, 637
Ecl136II GAGCTC 3 cut(s) 638, 1233, 1251
Eco24I GRGCYC 4 cut(s) 640, 1235, 1253, 1274
Eco47I GGWCC 1 cut(s) 2123
Eco53kI GAGCTC 3 cut(s) 638, 1233, 1251
Eco57I CTGAAG 3 cut(s) 846, 1290, 1407
Eco88I CYCGRG 1 cut(s) 1706
EcoICRI GAGCTC 3 cut(s) 638, 1233, 1251
EcoO109I RGGNCCY 1 cut(s) 2123
EcoRI GAATTC 2 cut(s) 2087, 2100
EcoRII CCWGG 1 cut(s) 1977
EcoT38I GRGCYC 4 cut(s) 640, 1235, 1253, 1274
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
Esp3I CGTCTC 2 cut(s) 625, 1380
FaeI CATG 1 cut(s) 1813
FaiI YATR 7 cut(s) 368, 1151, 1811, 1832, 1834, 1896, 2009
FaqI GGGAC 4 cut(s) 553, 784, 1522, 1679
FatI CATG 1 cut(s) 1809
FblI GTMKAC 1 cut(s) 1358
FokI GGATG 7 cut(s) 409, 484, 643, 796, 868, 1853, 1861
FriOI GRGCYC 4 cut(s) 640, 1235, 1253, 1274
FspBI CTAG 6 cut(s) 282, 1122, 1191, 1503, 1760, 1925
GlaI GCGC 3 cut(s) 181, 691, 766
GsaI CCCAGC 2 cut(s) 690, 765
GsuI CTGGAG 1 cut(s) 1098
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 7 cut(s) 99, 173, 507, 530, 1223, 1241, 2081
HapII CCGG 4 cut(s) 18, 150, 974, 1266
HgaI GACGC 1 cut(s) 738
HhaI GCGC 3 cut(s) 182, 692, 767
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 1 cut(s) 1813
Hin6I GCGC 3 cut(s) 180, 690, 765
HinP1I GCGC 3 cut(s) 180, 690, 765
HincII GTYRAC 1 cut(s) 1525
HindII GTYRAC 1 cut(s) 1525
HindIII AAGCTT 1 cut(s) 844
HinfI GANTC 7 cut(s) 363, 417, 483, 1111, 1168, 1774, 2034
HpaII CCGG 4 cut(s) 18, 150, 974, 1266
HphI GGTGA 1 cut(s) 1945
Hpy166II GTNNAC 3 cut(s) 144, 1359, 1525
Hpy188III TCNNGA 9 cut(s) 6, 18, 1090, 1308, 1616, 1708, 2097, 2138, 2169
Hpy8I GTNNAC 3 cut(s) 144, 1359, 1525
HpyCH4III ACNGT 5 cut(s) 1399, 1534, 1557, 1579, 1688
HpyCH4IV ACGT 3 cut(s) 140, 335, 1846
HpyF3I CTNAG 8 cut(s) 1182, 1252, 1470, 1608, 1700, 1824, 1905, 2051
HpySE526I ACGT 3 cut(s) 140, 335, 1846
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 1 cut(s) 1813
HspAI GCGC 3 cut(s) 180, 690, 765
KasI GGCGCC 1 cut(s) 179
Kpn2I TCCGGA 1 cut(s) 17
KroI GCCGGC 1 cut(s) 973
KroNI GCCGGC 1 cut(s) 975
Kzo9I GATC 7 cut(s) 20, 119, 164, 269, 1587, 1944, 2134
Lsp1109I GCAGC 9 cut(s) 424, 745, 913, 1186, 1268, 1450, 1505, 1858, 2116
LweI GCATC 3 cut(s) 506, 1876, 1883
MaeI CTAG 6 cut(s) 282, 1122, 1191, 1503, 1760, 1925
MaeII ACGT 3 cut(s) 140, 335, 1846
MaeIII GTNAC 2 cut(s) 1432, 1933
MalI GATC 7 cut(s) 22, 121, 166, 271, 1589, 1946, 2136
MbiI CCGCTC 2 cut(s) 1070, 1753
MboI GATC 7 cut(s) 20, 119, 164, 269, 1587, 1944, 2134
MflI RGATCY 4 cut(s) 20, 119, 164, 269
MhlI GDGCHC 4 cut(s) 640, 1235, 1253, 1274
MluCI AATT 7 cut(s) 1550, 1631, 1767, 1953, 2087, 2100, 2141
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 2 cut(s) 1105, 1768
MmeI TCCRAC 2 cut(s) 96, 848
MroI TCCGGA 1 cut(s) 17
MroNI GCCGGC 1 cut(s) 973
MseI TTAA 5 cut(s) 1766, 1967, 2039, 2060, 2144
MslI CAYNNNNRTG 2 cut(s) 1397, 1686
MspA1I CMGCKG 5 cut(s) 437, 736, 778, 926, 1199
MspI CCGG 4 cut(s) 18, 150, 974, 1266
MspR9I CCNGG 2 cut(s) 1267, 1979
Mva1269I GAATGC 1 cut(s) 1609
MvaI CCWGG 1 cut(s) 1979
MvnI CGCG 2 cut(s) 2098, 2139
NaeI GCCGGC 1 cut(s) 975
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 1 cut(s) 1267
NdeII GATC 7 cut(s) 20, 119, 164, 269, 1587, 1944, 2134
NgoMIV GCCGGC 1 cut(s) 973
NlaIII CATG 1 cut(s) 1813
NmeAIII GCCGAG 3 cut(s) 149, 506, 1214
NmuCI GTSAC 1 cut(s) 1933
NruI TCGCGA 2 cut(s) 2098, 2139
OliI CACNNNNGTG 2 cut(s) 1397, 1686
PaeR7I CTCGAG 1 cut(s) 1706
PaqCI CACCTGC 2 cut(s) 139, 1921
PcsI WCGNNNNNNNCGW 1 cut(s) 833
PctI GAATGC 1 cut(s) 1609
PdiI GCCGGC 1 cut(s) 975
PfeI GAWTC 5 cut(s) 363, 417, 483, 1168, 2034
PleI GAGTC 2 cut(s) 1105, 1768
PluTI GGCGCC 1 cut(s) 183
PpsI GAGTC 2 cut(s) 1105, 1768
PpuMI RGGWCCY 1 cut(s) 2123
PshBI ATTAAT 1 cut(s) 2144
Psp124BI GAGCTC 3 cut(s) 640, 1235, 1253
Psp5II RGGWCCY 1 cut(s) 2123
Psp6I CCWGG 1 cut(s) 1977
PspFI CCCAGC 2 cut(s) 686, 761
PspGI CCWGG 1 cut(s) 1977
PspPI GGNCC 4 cut(s) 98, 1222, 1240, 2123
PspPPI RGGWCCY 1 cut(s) 2123
PstI CTGCAG 1 cut(s) 2134
PstNI CAGNNNCTG 1 cut(s) 1430
PsuI RGATCY 4 cut(s) 20, 119, 164, 269
PvuII CAGCTG 1 cut(s) 736
RruI TCGCGA 2 cut(s) 2098, 2139
RsaI GTAC 3 cut(s) 1420, 1459, 1624
RsaNI GTAC 3 cut(s) 1419, 1458, 1623
RseI CAYNNNNRTG 2 cut(s) 1397, 1686
SacI GAGCTC 3 cut(s) 640, 1235, 1253
SaqAI TTAA 5 cut(s) 1766, 1967, 2039, 2060, 2144
Sau3AI GATC 7 cut(s) 20, 119, 164, 269, 1587, 1944, 2134
Sau96I GGNCC 4 cut(s) 98, 1222, 1240, 2123
ScaI AGTACT 1 cut(s) 1459
SchI GAGTC 2 cut(s) 1105, 1768
ScrFI CCNGG 2 cut(s) 1267, 1979
SduI GDGCHC 4 cut(s) 640, 1235, 1253, 1274
SfaNI GCATC 3 cut(s) 506, 1876, 1883
SfcI CTRYAG 3 cut(s) 816, 1530, 2130
SfoI GGCGCC 1 cut(s) 181
Sfr274I CTCGAG 1 cut(s) 1706
SinI GGWCC 1 cut(s) 2123
SlaI CTCGAG 1 cut(s) 1706
SmiMI CAYNNNNRTG 2 cut(s) 1397, 1686
SmlI CTYRAG 2 cut(s) 1377, 1706
SmoI CTYRAG 2 cut(s) 1377, 1706
SpeI ACTAGT 1 cut(s) 1502
Sse9I AATT 7 cut(s) 1550, 1631, 1767, 1953, 2087, 2100, 2141
SspDI GGCGCC 1 cut(s) 179
SspMI CTAG 6 cut(s) 282, 1122, 1191, 1503, 1760, 1925
SstI GAGCTC 3 cut(s) 640, 1235, 1253
StyD4I CCNGG 2 cut(s) 1265, 1977
TaaI ACNGT 5 cut(s) 1399, 1534, 1557, 1579, 1688
TaiI ACGT 3 cut(s) 143, 338, 1849
TaqI TCGA 2 cut(s) 1109, 1707
TasI AATT 7 cut(s) 1550, 1631, 1767, 1953, 2087, 2100, 2141
TatI WGTACW 1 cut(s) 1457
TauI GCSGC 8 cut(s) 431, 443, 497, 778, 1031, 1223, 1241, 1756
TfiI GAWTC 5 cut(s) 363, 417, 483, 1168, 2034
Tru1I TTAA 5 cut(s) 1766, 1967, 2039, 2060, 2144
Tru9I TTAA 5 cut(s) 1766, 1967, 2039, 2060, 2144
TseFI GTSAC 1 cut(s) 1933
TseI GCWGC 9 cut(s) 437, 733, 926, 1199, 1256, 1463, 1493, 1871, 2129
Tsp45I GTSAC 1 cut(s) 1933
TspDTI ATGAA 6 cut(s) 355, 645, 798, 1857, 2088, 2133
TspGWI ACGGA 5 cut(s) 555, 607, 1062, 1847, 2004
VpaK11BI GGWCC 1 cut(s) 2123
VspI ATTAAT 1 cut(s) 2144
XapI RAATTY 3 cut(s) 1631, 2087, 2100
XcmI CCANNNNNNNNNTGG 1 cut(s) 578
XhoI CTCGAG 1 cut(s) 1706
XmiI GTMKAC 1 cut(s) 1358
XspI CTAG 6 cut(s) 282, 1122, 1191, 1503, 1760, 1925
ZrmI AGTACT 1 cut(s) 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.