Rorug01G0194000

Nsp1-like C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003257
Physical Location & Seq
Reverse (-)
27036539 .. 27038670
2132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0194000.1

Sequence Viewer

Length: 552 bp
ATGGATAACAAAGACGATCCTAATGACAAAGACAAAGGGCTATTTTCAAATCTTGCTGGATATGCTACTGGACACTACCCACAGCATGGAGGATCATACCCTCCACAAGGATATGGGTATCCCCCACAAGGATATGGATACCCTCCCGCAGGCGGGTATCCTCCTCAAGGCGGCTATCCTCCGCAGGGTGGGTATCCTCCATCTGGATATCCTCCACAGGGTGGTTATCCTCCATCTGGATATCCTTCCCCTGGCGGATATCCACCAGCCGGGTATCCAGGTCCATCAGCTGCACATCATACAGGGCATGGATCGAACATGGGAGGCCTGCTAGCTGGGGGTGCTGCTGCTGCAGCTGCTGCTTATGGGGCTCACCATCTTGTGCACGGAAGTCACCACGGGGGTTACCATGGCTATGGTCACGGGAAGTTCAAGCATGGCAAACATGGGGGGTTTGGTCATGGGAAATTCAAGCATGGTAAACATGGGTTTGGCCATGGCAAATTTGGCAAGCGATTTGGATTTGGAGGCAAGTTCAAGAAATGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0003674 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005642 GO:0005643 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0008092 GO:0008104 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008285 GO:0008289 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010648 GO:0010928 GO:0010930 GO:0010941 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016043 GO:0016070 GO:0017038 GO:0017056 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019894 GO:0019904 GO:0019953 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030159 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032182 GO:0032386 GO:0032501 GO:0032504 GO:0032774 GO:0032879 GO:0032880 GO:0032947 GO:0032991 GO:0033036 GO:0033157 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0042127 GO:0042169 GO:0042175 GO:0042306 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043123 GO:0043130 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044613 GO:0044703 GO:0045184 GO:0045893 GO:0045935 GO:0046483 GO:0046822 GO:0046907 GO:0046966 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051223 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051259 GO:0051291 GO:0051427 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0060255 GO:0060341 GO:0060548 GO:0065003 GO:0065007 GO:0070013 GO:0070201 GO:0070206 GO:0070208 GO:0070727 GO:0071166 GO:0071426 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0098589 GO:0098805 GO:1900180 GO:1901360 GO:1901362 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1903827 GO:1904589 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nsp1_C Nucleoporin_FG

Protein Analysis

183

Amino Acids

18.83

Weight (kDa)

9.64

Isoelectric Point (pI)

31.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 86, 221
AciI CCGC 5 cut(s) 147, 153, 171, 182, 255
AclWI GGATC 3 cut(s) 11, 100, 319
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 2 cut(s) 467, 503
AdeI CACNNNGTG 1 cut(s) 221
AgsI TTSAA 4 cut(s) 48, 433, 472, 538
AjnI CCWGG 2 cut(s) 250, 277
AluBI AGCT 3 cut(s) 290, 335, 356
AluI AGCT 3 cut(s) 290, 335, 356
Alw21I GWGCWC 1 cut(s) 387
Alw44I GTGCAC 1 cut(s) 383
AlwI GGATC 3 cut(s) 11, 100, 319
AlwNI CAGNNNCTG 1 cut(s) 359
AoxI GGCC 2 cut(s) 325, 493
ApaLI GTGCAC 1 cut(s) 383
ApeKI GCWGC 7 cut(s) 290, 344, 347, 350, 353, 356, 359
ApoI RAATTY 2 cut(s) 467, 503
AspS9I GGNCC 1 cut(s) 281
AsuC2I CCSGG 1 cut(s) 271
AsuHPI GGTGA 2 cut(s) 365, 386
AsuNHI GCTAGC 1 cut(s) 331
AvaII GGWCC 1 cut(s) 281
BaeGI GKGCMC 1 cut(s) 387
BalI TGGCCA 1 cut(s) 495
BanII GRGCYC 1 cut(s) 373
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 7 cut(s) 277, 331, 334, 337, 343, 346, 365
BccI CCATC 4 cut(s) 208, 241, 292, 384
BciT130I CCWGG 2 cut(s) 252, 279
BciVI GTATCC 5 cut(s) 129, 131, 168, 204, 285
BcnI CCSGG 1 cut(s) 271
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 351
BfuI GTATCC 5 cut(s) 129, 131, 168, 204, 285
BisI GCNGC 8 cut(s) 172, 291, 345, 348, 351, 354, 357, 360
BlsI GCNGC 8 cut(s) 173, 292, 346, 349, 352, 355, 358, 361
Bme1390I CCNGG 3 cut(s) 252, 271, 279
Bme18I GGWCC 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 281
BmrFI CCNGG 3 cut(s) 252, 271, 279
BmtI GCTAGC 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 150
BpuMI CCSGG 1 cut(s) 271
BsaJI CCNNGG 4 cut(s) 250, 397, 409, 496
Bse1I ACTGG 1 cut(s) 73
BseBI CCWGG 2 cut(s) 252, 279
BseDI CCNNGG 4 cut(s) 250, 397, 409, 496
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 153
BseSI GKGCMC 1 cut(s) 387
BseXI GCAGC 7 cut(s) 277, 331, 334, 337, 343, 346, 365
BseYI CCCAGC 1 cut(s) 335
BsgI GTGCAG 1 cut(s) 276
BshFI GGCC 2 cut(s) 327, 495
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 1 cut(s) 270
BsnI GGCC 2 cut(s) 327, 495
Bsp1286I GDGCHC 2 cut(s) 373, 387
Bsp143I GATC 3 cut(s) 16, 92, 311
Bsp19I CCATGG 2 cut(s) 409, 496
BspACI CCGC 5 cut(s) 147, 153, 171, 182, 255
BspANI GGCC 2 cut(s) 327, 495
BspMAI CTGCAG 1 cut(s) 355
BspOI GCTAGC 1 cut(s) 335
BspPI GGATC 3 cut(s) 11, 100, 319
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 4 cut(s) 250, 397, 409, 496
BssMI GATC 3 cut(s) 16, 92, 311
BssT1I CCWWGG 2 cut(s) 409, 496
Bst2UI CCWGG 2 cut(s) 252, 279
BstAPI GCANNNNNTGC 1 cut(s) 359
BstC8I GCNNGC 4 cut(s) 151, 329, 333, 512
BstDSI CCRYGG 3 cut(s) 397, 409, 496
BstEII GGTNACC 1 cut(s) 404
BstENI CCTNNNNNAGG 3 cut(s) 105, 147, 165
BstKTI GATC 3 cut(s) 19, 95, 314
BstMBI GATC 3 cut(s) 16, 92, 311
BstMWI GCNNNNNNNGC 8 cut(s) 62, 341, 350, 353, 356, 359, 368, 507
BstNI CCWGG 2 cut(s) 252, 279
BstPI GGTNACC 1 cut(s) 404
BstSCI CCNGG 3 cut(s) 250, 269, 277
BstSFI CTRYAG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 387
BstV1I GCAGC 7 cut(s) 277, 331, 334, 337, 343, 346, 365
BstXI CCANNNNNNTGG 1 cut(s) 416
BsuI GTATCC 5 cut(s) 129, 131, 168, 204, 285
BsuRI GGCC 2 cut(s) 327, 495
BtgI CCRYGG 3 cut(s) 397, 409, 496
Cac8I GCNNGC 4 cut(s) 151, 329, 333, 512
CaiI CAGNNNCTG 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 281
DpnI GATC 3 cut(s) 18, 94, 313
DpnII GATC 3 cut(s) 16, 92, 311
DraIII CACNNNGTG 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 493
EciI GGCGGA 1 cut(s) 270
Eco130I CCWWGG 2 cut(s) 409, 496
Eco147I AGGCCT 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 373
Eco32I GATATC 3 cut(s) 209, 242, 260
Eco47I GGWCC 1 cut(s) 281
Eco91I GGTNACC 1 cut(s) 404
EcoNI CCTNNNNNAGG 3 cut(s) 105, 147, 165
EcoO65I GGTNACC 1 cut(s) 404
EcoRII CCWGG 2 cut(s) 250, 277
EcoRV GATATC 3 cut(s) 209, 242, 260
EcoT14I CCWWGG 2 cut(s) 409, 496
EcoT38I GRGCYC 1 cut(s) 373
ErhI CCWWGG 2 cut(s) 409, 496
FauI CCCGC 2 cut(s) 146, 154
Fnu4HI GCNGC 8 cut(s) 172, 291, 345, 348, 351, 354, 357, 360
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 8 cut(s) 172, 291, 345, 348, 351, 354, 357, 360
FspBI CTAG 1 cut(s) 332
GluI GCNGC 8 cut(s) 172, 291, 345, 348, 351, 354, 357, 360
GsaI CCCAGC 1 cut(s) 339
HaeIII GGCC 2 cut(s) 327, 495
HapII CCGG 1 cut(s) 270
HpaII CCGG 1 cut(s) 270
HphI GGTGA 2 cut(s) 365, 386
Hpy166II GTNNAC 2 cut(s) 385, 482
Hpy188III TCNNGA 3 cut(s) 204, 237, 538
Hpy8I GTNNAC 2 cut(s) 385, 482
HpyAV CCTTC 1 cut(s) 255
HpyCH4V TGCA 3 cut(s) 293, 353, 385
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 341, 350, 353, 356, 359, 368, 507
Kzo9I GATC 3 cut(s) 16, 92, 311
Lsp1109I GCAGC 7 cut(s) 277, 331, 334, 337, 343, 346, 365
MaeI CTAG 1 cut(s) 332
MaeIII GTNAC 3 cut(s) 392, 404, 419
MalI GATC 3 cut(s) 18, 94, 313
MboI GATC 3 cut(s) 16, 92, 311
MhlI GDGCHC 2 cut(s) 373, 387
MlsI TGGCCA 1 cut(s) 495
MluCI AATT 2 cut(s) 467, 503
MluNI TGGCCA 1 cut(s) 495
Mox20I TGGCCA 1 cut(s) 495
MscI TGGCCA 1 cut(s) 495
MslI CAYNNNNRTG 1 cut(s) 414
Msp20I TGGCCA 1 cut(s) 495
MspA1I CMGCKG 2 cut(s) 290, 356
MspI CCGG 1 cut(s) 270
MspR9I CCNGG 3 cut(s) 252, 271, 279
MvaI CCWGG 2 cut(s) 252, 279
MwoI GCNNNNNNNGC 8 cut(s) 62, 341, 350, 353, 356, 359, 368, 507
NciI CCSGG 1 cut(s) 271
NcoI CCATGG 2 cut(s) 409, 496
NdeII GATC 3 cut(s) 16, 92, 311
NheI GCTAGC 1 cut(s) 331
NmuCI GTSAC 2 cut(s) 392, 419
PceI AGGCCT 1 cut(s) 327
PflMI CCANNNNNTGG 2 cut(s) 86, 221
PkrI GCNGC 8 cut(s) 173, 292, 346, 349, 352, 355, 358, 361
Psp6I CCWGG 2 cut(s) 250, 277
PspEI GGTNACC 1 cut(s) 404
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 2 cut(s) 250, 277
PspPI GGNCC 1 cut(s) 281
PstI CTGCAG 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 359
PvuII CAGCTG 2 cut(s) 290, 356
RseI CAYNNNNRTG 1 cut(s) 414
SatI GCNGC 8 cut(s) 172, 291, 345, 348, 351, 354, 357, 360
Sau3AI GATC 3 cut(s) 16, 92, 311
Sau96I GGNCC 1 cut(s) 281
ScrFI CCNGG 3 cut(s) 252, 271, 279
SduI GDGCHC 2 cut(s) 373, 387
SetI ASST 4 cut(s) 283, 292, 337, 358
SfcI CTRYAG 1 cut(s) 351
SinI GGWCC 1 cut(s) 281
SmiMI CAYNNNNRTG 1 cut(s) 414
SmlI CTYRAG 1 cut(s) 165
SmoI CTYRAG 1 cut(s) 165
Sse9I AATT 2 cut(s) 467, 503
SseBI AGGCCT 1 cut(s) 327
SsiI CCGC 5 cut(s) 147, 153, 171, 182, 255
SspMI CTAG 1 cut(s) 332
StuI AGGCCT 1 cut(s) 327
StyD4I CCNGG 3 cut(s) 250, 269, 277
StyI CCWWGG 2 cut(s) 409, 496
TaqI TCGA 1 cut(s) 314
TasI AATT 2 cut(s) 467, 503
TauI GCSGC 1 cut(s) 174
TseFI GTSAC 2 cut(s) 392, 419
TseI GCWGC 7 cut(s) 290, 344, 347, 350, 353, 356, 359
Tsp45I GTSAC 2 cut(s) 392, 419
TspGWI ACGGA 1 cut(s) 402
Van91I CCANNNNNTGG 2 cut(s) 86, 221
VneI GTGCAC 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 281
XagI CCTNNNNNAGG 3 cut(s) 105, 147, 165
XapI RAATTY 2 cut(s) 467, 503
XcmI CCANNNNNNNNNTGG 1 cut(s) 503
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.