RLG00000035993

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003257
Physical Location & Seq
Forward (+)
74636420 .. 74637874
1455 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035993

Sequence Viewer

Length: 387 bp
ATGAAGCCTATGAATGCTGGGACAAGTAGAGCTAAGAAGGAGTTGAAGCACCTGAAGGTTTTGGGGTTGGCAGTGATTTACATAATGATGTTTGTATTGAATCTGTACGAGTACACGAAGAAGAACTTGGGGCCTCTGAGATTGATTGTGTGGACTGTGGAGGTTGCTATGATCAATATTATTGCTCCAATCTACTGGAGATTCGAAGTTGATATGATCAATATTGTTGCTCCAATCTCTCGGAGATTCGAAGGAGTGCCTGATGATTTCCTTGGTTTACTCGACACGAAGGTAATATGGGCCAATCATGTTCTTGCAATTAGGCTCGAGGCTTACTTTAGTAGTCTCCTCAGCCGAAATGGCAAGAGAGGTTTTGAAAACTCATGA

Protein Analysis

129

Amino Acids

14.73

Weight (kDa)

9.74

Isoelectric Point (pI)

20.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 11 - 69 1.1e-12 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 74
AfaI GTAC 2 cut(s) 107, 113
AgsI TTSAA 3 cut(s) 46, 100, 377
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 1 cut(s) 32
AluI AGCT 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 2 cut(s) 131, 300
AspS9I GGNCC 2 cut(s) 131, 300
AsuII TTCGAA 2 cut(s) 204, 249
AvaI CYCGRG 1 cut(s) 326
BbvCI CCTCAGC 1 cut(s) 350
BclI TGATCA 2 cut(s) 171, 216
BcoDI GTCTC 1 cut(s) 350
BglI GCCNNNNNGGC 1 cut(s) 360
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 2 cut(s) 131, 300
BmiI GGNNCC 1 cut(s) 132
BpmI CTGGAG 1 cut(s) 217
Bpu10I CCTNAGC 1 cut(s) 350
Bpu14I TTCGAA 2 cut(s) 204, 249
BsaJI CCNNGG 1 cut(s) 271
Bse1I ACTGG 1 cut(s) 200
BseDI CCNNGG 1 cut(s) 271
BseMII CTCAG 2 cut(s) 128, 364
BseNI ACTGG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 338
BseYI CCCAGC 1 cut(s) 17
BshFI GGCC 2 cut(s) 133, 302
BsiHKCI CYCGRG 1 cut(s) 326
BslFI GGGAC 1 cut(s) 34
BsmAI GTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 19
BsnI GGCC 2 cut(s) 133, 302
BsoBI CYCGRG 1 cut(s) 326
Bsp119I TTCGAA 2 cut(s) 204, 249
Bsp143I GATC 2 cut(s) 171, 216
BspANI GGCC 2 cut(s) 133, 302
BspCNI CTCAG 2 cut(s) 129, 363
BspHI TCATGA 1 cut(s) 383
BspLI GGNNCC 1 cut(s) 132
BspT104I TTCGAA 2 cut(s) 204, 249
BsrI ACTGG 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 2 cut(s) 171, 216
BssT1I CCWWGG 1 cut(s) 271
Bst4CI ACNGT 1 cut(s) 157
BstBI TTCGAA 2 cut(s) 204, 249
BstDEI CTNAG 3 cut(s) 33, 137, 350
BstKTI GATC 2 cut(s) 174, 219
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 2 cut(s) 171, 216
BstMWI GCNNNNNNNGC 1 cut(s) 360
BstXI CCANNNNNNTGG 1 cut(s) 195
BsuRI GGCC 2 cut(s) 133, 302
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 78
CciI TCATGA 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 131, 300
Csp6I GTAC 2 cut(s) 106, 112
CviAII CATG 2 cut(s) 308, 384
CviJI RGCY 7 cut(s) 7, 32, 133, 302, 325, 332, 354
CviKI_1 RGCY 7 cut(s) 7, 32, 133, 302, 325, 332, 354
CviQI GTAC 2 cut(s) 106, 112
DdeI CTNAG 3 cut(s) 33, 137, 350
DpnI GATC 2 cut(s) 173, 218
DpnII GATC 2 cut(s) 171, 216
Eco130I CCWWGG 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 74
Eco88I CYCGRG 1 cut(s) 326
EcoO109I RGGNCCY 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 2 cut(s) 311, 387
FaiI YATR 7 cut(s) 11, 83, 170, 215, 298, 309, 385
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 307, 383
FbaI TGATCA 2 cut(s) 171, 216
GsaI CCCAGC 1 cut(s) 21
GsuI CTGGAG 1 cut(s) 217
HaeIII GGCC 2 cut(s) 133, 302
Hin1II CATG 2 cut(s) 311, 387
HinfI GANTC 3 cut(s) 100, 201, 246
Hpy166II GTNNAC 3 cut(s) 114, 153, 278
Hpy188I TCNGA 2 cut(s) 138, 243
Hpy188III TCNNGA 1 cut(s) 384
Hpy8I GTNNAC 3 cut(s) 114, 153, 278
HpyAV CCTTC 4 cut(s) 31, 49, 245, 283
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4V TGCA 1 cut(s) 317
HpyF10VI GCNNNNNNNGC 1 cut(s) 360
HpyF3I CTNAG 3 cut(s) 33, 137, 350
Hsp92II CATG 2 cut(s) 311, 387
Ksp22I TGATCA 2 cut(s) 171, 216
Kzo9I GATC 2 cut(s) 171, 216
LmnI GCTCC 2 cut(s) 190, 235
LpnPI CCDG 4 cut(s) 3, 65, 181, 273
MalI GATC 2 cut(s) 173, 218
MboI GATC 2 cut(s) 171, 216
MboII GAAGA 2 cut(s) 130, 133
MluCI AATT 1 cut(s) 318
MnlI CCTC 5 cut(s) 144, 154, 322, 359, 362
MslI CAYNNNNRTG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 19
MwoI GCNNNNNNNGC 1 cut(s) 360
NdeII GATC 2 cut(s) 171, 216
NlaIII CATG 2 cut(s) 311, 387
NlaIV GGNNCC 1 cut(s) 132
NspV TTCGAA 2 cut(s) 204, 249
PaeR7I CTCGAG 1 cut(s) 326
PagI TCATGA 1 cut(s) 383
PctI GAATGC 1 cut(s) 19
PfeI GAWTC 3 cut(s) 100, 201, 246
PspFI CCCAGC 1 cut(s) 17
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 2 cut(s) 131, 300
PspXI VCTCGAGB 1 cut(s) 326
RsaI GTAC 2 cut(s) 107, 113
RsaNI GTAC 2 cut(s) 106, 112
RseI CAYNNNNRTG 1 cut(s) 86
Sau3AI GATC 2 cut(s) 171, 216
Sau96I GGNCC 2 cut(s) 131, 300
SetI ASST 6 cut(s) 34, 54, 60, 165, 294, 373
Sfr274I CTCGAG 1 cut(s) 326
SfuI TTCGAA 2 cut(s) 204, 249
SlaI CTCGAG 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 1 cut(s) 318
SspI AATATT 2 cut(s) 178, 223
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 1 cut(s) 157
TaqI TCGA 4 cut(s) 204, 249, 282, 327
TasI AATT 1 cut(s) 318
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 3 cut(s) 100, 201, 246
TscAI CASTG 1 cut(s) 78
TspDTI ATGAA 2 cut(s) 17, 26
TspRI CASTG 1 cut(s) 78
XhoI CTCGAG 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.