Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003257

Physical Location & Seq

Genomic Coordinates Forward (+)

242329 .. 245097

Gene Structure 2769 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1004200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1224 bp

ATGTTGGGTGGTGGTGGTGGCTGCTCTCGTCTTTCCACCCATCTGCGACTTCGAGGCTCGGACATCAACCGGCTCCACGTCAACCTCCACCACCCGCAGCTAGGCTTGGGCTTTGGCTTTGTGTTTGTCTTGGGATTAGGGTCTTACAACACCAGAATCGCCCACCTCCGCTGCTGCCCCTGCCCTCCACCTCTCAAACCCCTATCCTTGTCGTCTGCCGTCTCTCACTCTCACACACAGACTCACACAAGGGGGATTCCTTTTCCCTCCATCCTTGCTTGTTCTAAGTTGTCTTCCTCCAACATTACCGCCCTCTCCCATTGTTTTAGCCAACGAGAAGGTGAACGCGAGCTTCCCCCCGAAGGATTGTCCTCTGTCGCAGGAGGAATAGTCGCACTGGGAAAATTCGATGCCCTTCACATTGGTCATCGAGAACTTGCGATTCAAGCATCCAAAGTTGGACCTCCATTCCTTTTGTCATTTGTTGGAATGGCTGAAGTACTGGGTTGGGAACCAAGAGCTCCCATAGTTGCGAAATGTGATCGCAAACGGGTTCTTTCCTCTTGGGCCCCGTACTGTGGTGACATGGCACCTGCAGAGTTTCAGATTGAATTTTCAAGTGTTCGATGTCTAACACCAAGGCAATTTGTTGAGAAATTAGTGAAGGAGCTTGGAGTGCGTGGGGTTGTCACAGGTGAAAACTACCGATTTGGATACAAAGCTGCAGGTGATGCGACAGAGCTGGTGAGGTTGTGTGAAGAGTATGGCATTGAGGCATATATCATAAATTCTGTTATGGACAAGAACGAATATTCCATAAATATACAGTCGAGTGATTTGAAAGACAGAGGACAAGTCTCCTCTACTCGCGTTCGCCGTGCCCTTGCTGTGGGAGATATGAAGTATGTCTCACAACTGTTGGGTCGGCAGCATCGTCTGATAATGGGTGCTGCAAAGGACCAGGAAGGATTTACTGGCAGCAAACACAAGTTGTCAGTTCCAAAGTCTGGTTTGTTAAATCTGGCTCCAAAGGAGGGTCTATACGAGAAGTGTTATCTTTGTAGTGGGGAGGATGATGACCTAGCATCATCCTGCAGGGTCGTGGTTGACACCGGGAATGTCCATATAGAAATGGATGATGAGGTAGACGTAGGCGTATGTAATAGAGTTGGTACACAGGACTTGAGGCTCTTACGTATTGAATTTGGTGATTCGCAATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003919 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006732 GO:0006747 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009165 GO:0009507 GO:0009536 GO:0009987 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0019637 GO:0034641 GO:0034654 GO:0042726 GO:0042727 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046443 GO:0046483 GO:0051186 GO:0051188 GO:0055086 GO:0070566 GO:0071704 GO:0072387 GO:0072388 GO:0090407 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_syn

Protein Analysis

408

Amino Acids

44.34

Weight (kDa)

8.16

Isoelectric Point (pI)

39.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_syn	PF06574	128 - 270	3.4e-15	FAD synthetase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08340 AT5G08340 AT5G23330
fragaria_vesca	FvH4_4g37140
malus_domestica	MD13G1003200.v1.1 MD16G1004200.v1.1
prunus_persica	Prupe.1G349200_v2.0.a1 Prupe.1G349200_v2.0.a1 Prupe.1G349200_v2.0.a1 Prupe.1G349200_v2.0.a1
pyrus_communis	pycom13g00270 pycom16g00180
rosa_chinensis	RchiOBHm_Chr2g0144991 RchiOBHm_Chr4g0446941 RchiOBHm_Chr5g0081501 RchiOBHm_Chr6g0272191 RchiOBHm_Chr7g0231651
rosa_laevigata	RLG00000001051 RLG00000020160 RLG00000035583 RLG00000035993
rosa_multiflora	Rmu_co8227451.1_g000001 Rmu_sc0000015.1_g000048 Rmu_sc0002784.1_g000009
rosa_roxburghii	Rroxscaffold_3G00229270 Rroxscaffold_3G00237760
rosa_rugosa	Rorug01G0193900 Rorug01G0194000
rosa_samantha	Rh2DG102300 Rh4AG437800 Rh4BG446700 Rh4CG464000 Rh4DG445400 Rh5AG442200 Rh5DG474200 Rh6BG133900
rosa_wichuraiana	Rw4G037440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	601, 716
Acc36I	ACCTGC	2 cut(s)	601, 716
AccB1I	GGYRCC	1 cut(s)	589
AccB7I	CCANNNNNTGG	1 cut(s)	1007
AccI	GTMKAC	1 cut(s)	1146
AccII	CGCG	2 cut(s)	348, 870
AciI	CCGC	3 cut(s)	95, 169, 309
AcsI	RAATTY	4 cut(s)	404, 611, 787, 1202
AcuI	CTGAAG	1 cut(s)	516
AfaI	GTAC	3 cut(s)	501, 575, 1174
AfiI	CCNNNNNNNGG	6 cut(s)	101, 362, 578, 889, 1007, 1034
AgsI	TTSAA	5 cut(s)	446, 611, 618, 841, 1202
AjiI	CACGTC	1 cut(s)	79
AjnI	CCWGG	1 cut(s)	960
AloI	GAACNNNNNNTCC	2 cut(s)	797, 829
AluBI	AGCT	6 cut(s)	100, 352, 521, 670, 722, 742
AluI	AGCT	6 cut(s)	100, 352, 521, 670, 722, 742
Alw21I	GWGCWC	1 cut(s)	523
Alw26I	GTCTC	3 cut(s)	226, 862, 913
AoxI	GGCC	1 cut(s)	567
ApaI	GGGCCC	1 cut(s)	571
ApeKI	GCWGC	8 cut(s)	21, 97, 171, 174, 722, 928, 950, 978
ApoI	RAATTY	4 cut(s)	404, 611, 787, 1202
AspS9I	GGNCC	4 cut(s)	461, 567, 568, 958
AsuC2I	CCSGG	1 cut(s)	1114
AsuHPI	GGTGA	6 cut(s)	353, 593, 707, 740, 757, 1220
AvaII	GGWCC	2 cut(s)	461, 958
BaeGI	GKGCMC	2 cut(s)	571, 883
BanI	GGYRCC	1 cut(s)	589
BanII	GRGCYC	2 cut(s)	523, 571
BbsI	GAAGAC	1 cut(s)	285
Bbv12I	GWGCWC	1 cut(s)	523
BbvI	GCAGC	8 cut(s)	8, 109, 158, 161, 709, 937, 940, 990
BccI	CCATC	2 cut(s)	48, 278
BceAI	ACGGC	2 cut(s)	203, 861
BciT130I	CCWGG	1 cut(s)	962
BciVI	GTATCC	1 cut(s)	707
BcnI	CCSGG	1 cut(s)	1114
BcoDI	GTCTC	3 cut(s)	226, 862, 913
BfaI	CTAG	2 cut(s)	101, 1082
BfmI	CTRYAG	3 cut(s)	594, 723, 1093
BfuAI	ACCTGC	2 cut(s)	601, 716
BfuI	GTATCC	1 cut(s)	707
BisI	GCNGC	8 cut(s)	22, 98, 172, 175, 723, 929, 951, 979
BlsI	GCNGC	8 cut(s)	23, 99, 173, 176, 724, 930, 952, 980
BmcAI	AGTACT	1 cut(s)	501
Bme1390I	CCNGG	2 cut(s)	962, 1114
Bme18I	GGWCC	2 cut(s)	461, 958
BmgBI	CACGTC	1 cut(s)	79
BmgT120I	GGNCC	4 cut(s)	461, 567, 568, 958
BmiI	GGNNCC	6 cut(s)	74, 513, 569, 570, 591, 1026
BmrFI	CCNGG	2 cut(s)	962, 1114
BmrI	ACTGGG	2 cut(s)	407, 512
BmsI	GCATC	5 cut(s)	400, 458, 721, 940, 1094
BmuI	ACTGGG	2 cut(s)	407, 512
BpiI	GAAGAC	1 cut(s)	285
BpuEI	CTTGAG	1 cut(s)	1204
BpuMI	CCSGG	1 cut(s)	1114
BsaAI	YACGTR	1 cut(s)	1196
BsaJI	CCNNGG	1 cut(s)	638
BsaXI	ACNNNNNCTCC	2 cut(s)	375, 405
Bsc4I	CCNNNNNNNGG	6 cut(s)	101, 362, 578, 889, 1007, 1034
Bse118I	RCCGGY	1 cut(s)	69
Bse1I	ACTGG	3 cut(s)	402, 507, 979
BseBI	CCWGG	1 cut(s)	962
BseDI	CCNNGG	1 cut(s)	638
BseGI	GGATG	5 cut(s)	270, 449, 1078, 1088, 1141
BseLI	CCNNNNNNNGG	6 cut(s)	101, 362, 578, 889, 1007, 1034
BseNI	ACTGG	3 cut(s)	402, 507, 979
BseRI	GAGGAG	1 cut(s)	850
BseSI	GKGCMC	2 cut(s)	571, 883
BseXI	GCAGC	8 cut(s)	8, 109, 158, 161, 709, 937, 940, 990
Bsh1236I	CGCG	2 cut(s)	348, 870
BshFI	GGCC	1 cut(s)	569
BshNI	GGYRCC	1 cut(s)	589
BsiHKAI	GWGCWC	1 cut(s)	523
BsiSI	CCGG	2 cut(s)	70, 1113
BslI	CCNNNNNNNGG	6 cut(s)	101, 362, 578, 889, 1007, 1034
BsmAI	GTCTC	3 cut(s)	226, 862, 913
BsmBI	CGTCTC	1 cut(s)	226
BsnI	GGCC	1 cut(s)	569
Bsp120I	GGGCCC	1 cut(s)	567
Bsp1286I	GDGCHC	3 cut(s)	523, 571, 883
Bsp143I	GATC	1 cut(s)	541
BspACI	CCGC	3 cut(s)	95, 169, 309
BspANI	GGCC	1 cut(s)	569
BspFNI	CGCG	2 cut(s)	348, 870
BspLI	GGNNCC	6 cut(s)	74, 513, 569, 570, 591, 1026
BspMAI	CTGCAG	3 cut(s)	598, 727, 1097
BspMI	ACCTGC	2 cut(s)	601, 716
BspT107I	GGYRCC	1 cut(s)	589
BsrFI	RCCGGY	1 cut(s)	69
BsrI	ACTGG	3 cut(s)	402, 507, 979
BssAI	RCCGGY	1 cut(s)	69
BssECI	CCNNGG	1 cut(s)	638
BssMI	GATC	1 cut(s)	541
BssT1I	CCWWGG	1 cut(s)	638
Bst2UI	CCWGG	1 cut(s)	962
Bst4CI	ACNGT	3 cut(s)	578, 828, 918
Bst6I	CTCTTC	1 cut(s)	753
BstAPI	GCANNNNNTGC	1 cut(s)	731
BstBAI	YACGTR	1 cut(s)	1196
BstC8I	GCNNGC	1 cut(s)	350
BstDEI	CTNAG	1 cut(s)	285
BstF5I	GGATG	5 cut(s)	270, 449, 1078, 1088, 1141
BstFNI	CGCG	2 cut(s)	348, 870
BstKTI	GATC	1 cut(s)	544
BstMAI	GTCTC	3 cut(s)	226, 862, 913
BstMBI	GATC	1 cut(s)	541
BstMWI	GCNNNNNNNGC	4 cut(s)	180, 446, 676, 731
BstNI	CCWGG	1 cut(s)	962
BstSCI	CCNGG	2 cut(s)	960, 1112
BstSFI	CTRYAG	3 cut(s)	594, 723, 1093
BstSLI	GKGCMC	2 cut(s)	571, 883
BstSNI	TACGTA	1 cut(s)	1196
BstUI	CGCG	2 cut(s)	348, 870
BstV1I	GCAGC	8 cut(s)	8, 109, 158, 161, 709, 937, 940, 990
BstV2I	GAAGAC	1 cut(s)	285
BsuI	GTATCC	1 cut(s)	707
BsuRI	GGCC	1 cut(s)	569
BtrI	CACGTC	1 cut(s)	79
BtsCI	GGATG	5 cut(s)	270, 449, 1078, 1088, 1141
BtsIMutI	CAGTG	1 cut(s)	395
BveI	ACCTGC	2 cut(s)	601, 716
Cac8I	GCNNGC	1 cut(s)	350
Cfr10I	RCCGGY	1 cut(s)	69
Cfr13I	GGNCC	4 cut(s)	461, 567, 568, 958
Csp6I	GTAC	3 cut(s)	500, 574, 1173
CviAII	CATG	1 cut(s)	586
CviQI	GTAC	3 cut(s)	500, 574, 1173
DdeI	CTNAG	1 cut(s)	285
DpnI	GATC	1 cut(s)	543
DpnII	GATC	1 cut(s)	541
Eam1104I	CTCTTC	1 cut(s)	753
EarI	CTCTTC	1 cut(s)	753
Ecl136II	GAGCTC	1 cut(s)	521
Eco105I	TACGTA	1 cut(s)	1196
Eco130I	CCWWGG	1 cut(s)	638
Eco24I	GRGCYC	2 cut(s)	523, 571
Eco47I	GGWCC	2 cut(s)	461, 958
Eco53kI	GAGCTC	1 cut(s)	521
Eco57I	CTGAAG	1 cut(s)	516
EcoICRI	GAGCTC	1 cut(s)	521
EcoO109I	RGGNCCY	1 cut(s)	568
EcoRII	CCWGG	1 cut(s)	960
EcoT14I	CCWWGG	1 cut(s)	638
EcoT38I	GRGCYC	2 cut(s)	523, 571
ErhI	CCWWGG	1 cut(s)	638
Esp3I	CGTCTC	1 cut(s)	226
FaeI	CATG	1 cut(s)	589
FatI	CATG	1 cut(s)	585
FauI	CCCGC	1 cut(s)	102
FblI	GTMKAC	1 cut(s)	1146
Fnu4HI	GCNGC	8 cut(s)	22, 98, 172, 175, 723, 929, 951, 979
FokI	GGATG	5 cut(s)	257, 436, 1075, 1085, 1148
FriOI	GRGCYC	2 cut(s)	523, 571
Fsp4HI	GCNGC	8 cut(s)	22, 98, 172, 175, 723, 929, 951, 979
FspBI	CTAG	2 cut(s)	101, 1082
GluI	GCNGC	8 cut(s)	22, 98, 172, 175, 723, 929, 951, 979
HaeIII	GGCC	1 cut(s)	569
HapII	CCGG	2 cut(s)	70, 1113
Hin1II	CATG	1 cut(s)	589
HincII	GTYRAC	2 cut(s)	82, 1108
HindII	GTYRAC	2 cut(s)	82, 1108
HinfI	GANTC	5 cut(s)	156, 241, 256, 442, 1211
HpaII	CCGG	2 cut(s)	70, 1113
HphI	GGTGA	6 cut(s)	353, 593, 707, 740, 757, 1220
Hpy166II	GTNNAC	5 cut(s)	82, 344, 1108, 1147, 1175
Hpy188I	TCNGA	3 cut(s)	61, 606, 939
Hpy188III	TCNNGA	2 cut(s)	431, 1221
Hpy8I	GTNNAC	5 cut(s)	82, 344, 1108, 1147, 1175
HpyAV	CCTTC	5 cut(s)	332, 356, 425, 658, 959
HpyCH4III	ACNGT	3 cut(s)	578, 828, 918
HpyCH4IV	ACGT	3 cut(s)	78, 1149, 1195
HpyCH4V	TGCA	4 cut(s)	596, 725, 953, 1095
HpyF10VI	GCNNNNNNNGC	4 cut(s)	180, 446, 676, 731
HpyF3I	CTNAG	1 cut(s)	285
HpySE526I	ACGT	3 cut(s)	78, 1149, 1195
Hsp92II	CATG	1 cut(s)	589
Kzo9I	GATC	1 cut(s)	541
LmnI	GCTCC	4 cut(s)	78, 526, 667, 1030
Lsp1109I	GCAGC	8 cut(s)	8, 109, 158, 161, 709, 937, 940, 990
LweI	GCATC	5 cut(s)	400, 458, 721, 940, 1094
MaeI	CTAG	2 cut(s)	101, 1082
MaeII	ACGT	3 cut(s)	78, 1149, 1195
MaeIII	GTNAC	2 cut(s)	581, 688
MalI	GATC	1 cut(s)	543
MboI	GATC	1 cut(s)	541
MboII	GAAGA	2 cut(s)	285, 770
MhlI	GDGCHC	3 cut(s)	523, 571, 883
MluCI	AATT	6 cut(s)	404, 611, 644, 656, 787, 1202
MlyI	GAGTC	1 cut(s)	235
MmeI	TCCRAC	3 cut(s)	324, 439, 466
MseI	TTAA	1 cut(s)	1016
MspA1I	CMGCKG	1 cut(s)	171
MspI	CCGG	2 cut(s)	70, 1113
MspR9I	CCNGG	2 cut(s)	962, 1114
MvaI	CCWGG	1 cut(s)	962
MvnI	CGCG	2 cut(s)	348, 870
MwoI	GCNNNNNNNGC	4 cut(s)	180, 446, 676, 731
NciI	CCSGG	1 cut(s)	1114
NdeII	GATC	1 cut(s)	541
NlaIII	CATG	1 cut(s)	589
NlaIV	GGNNCC	6 cut(s)	74, 513, 569, 570, 591, 1026
NmuCI	GTSAC	2 cut(s)	581, 688
PaqCI	CACCTGC	2 cut(s)	601, 716
PcsI	WCGNNNNNNNCGW	2 cut(s)	874, 931
PfeI	GAWTC	4 cut(s)	156, 256, 442, 1211
PflMI	CCANNNNNTGG	1 cut(s)	1007
PkrI	GCNGC	8 cut(s)	23, 99, 173, 176, 724, 930, 952, 980
PleI	GAGTC	1 cut(s)	235
PpsI	GAGTC	1 cut(s)	235
Ppu21I	YACGTR	1 cut(s)	1196
Psp124BI	GAGCTC	1 cut(s)	523
Psp6I	CCWGG	1 cut(s)	960
PspGI	CCWGG	1 cut(s)	960
PspN4I	GGNNCC	6 cut(s)	74, 513, 569, 570, 591, 1026
PspOMI	GGGCCC	1 cut(s)	567
PspPI	GGNCC	4 cut(s)	461, 567, 568, 958
PstI	CTGCAG	3 cut(s)	598, 727, 1097
RsaI	GTAC	3 cut(s)	501, 575, 1174
RsaNI	GTAC	3 cut(s)	500, 574, 1173
SacI	GAGCTC	1 cut(s)	523
SaqAI	TTAA	1 cut(s)	1016
SatI	GCNGC	8 cut(s)	22, 98, 172, 175, 723, 929, 951, 979
Sau3AI	GATC	1 cut(s)	541
Sau96I	GGNCC	4 cut(s)	461, 567, 568, 958
SbfI	CCTGCAGG	1 cut(s)	1097
ScaI	AGTACT	1 cut(s)	501
SchI	GAGTC	1 cut(s)	235
ScrFI	CCNGG	2 cut(s)	962, 1114
SdaI	CCTGCAGG	1 cut(s)	1097
SduI	GDGCHC	3 cut(s)	523, 571, 883
SfaNI	GCATC	5 cut(s)	400, 458, 721, 940, 1094
SfcI	CTRYAG	3 cut(s)	594, 723, 1093
SinI	GGWCC	2 cut(s)	461, 958
SmlI	CTYRAG	1 cut(s)	1183
SmoI	CTYRAG	1 cut(s)	1183
SnaBI	TACGTA	1 cut(s)	1196
Sse8387I	CCTGCAGG	1 cut(s)	1097
Sse9I	AATT	6 cut(s)	404, 611, 644, 656, 787, 1202
SsiI	CCGC	3 cut(s)	95, 169, 309
SspI	AATATT	1 cut(s)	812
SspMI	CTAG	2 cut(s)	101, 1082
SstI	GAGCTC	1 cut(s)	523
StyD4I	CCNGG	2 cut(s)	960, 1112
StyI	CCWWGG	1 cut(s)	638
TaaI	ACNGT	3 cut(s)	578, 828, 918
TaiI	ACGT	3 cut(s)	81, 1152, 1198
TaqI	TCGA	5 cut(s)	52, 408, 430, 625, 830
TasI	AATT	6 cut(s)	404, 611, 644, 656, 787, 1202
TatI	WGTACW	1 cut(s)	499
TfiI	GAWTC	4 cut(s)	156, 256, 442, 1211
Tru1I	TTAA	1 cut(s)	1016
Tru9I	TTAA	1 cut(s)	1016
TscAI	CASTG	1 cut(s)	402
TseFI	GTSAC	2 cut(s)	581, 688
TseI	GCWGC	8 cut(s)	21, 97, 171, 174, 722, 928, 950, 978
Tsp45I	GTSAC	2 cut(s)	581, 688
TspDTI	ATGAA	1 cut(s)	914
TspRI	CASTG	1 cut(s)	402
Van91I	CCANNNNNTGG	1 cut(s)	1007
VpaK11BI	GGWCC	2 cut(s)	461, 958
XapI	RAATTY	4 cut(s)	404, 611, 787, 1202
XmiI	GTMKAC	1 cut(s)	1146
XspI	CTAG	2 cut(s)	101, 1082
ZrmI	AGTACT	1 cut(s)	501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.