RLG00000020160

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003257
Physical Location & Seq
Forward (+)
61875411 .. 61876508
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020160

Sequence Viewer

Length: 420 bp
ATGAAGCCTATGAATGCTGGGACAAGCAGAGCTAAGAAGGAGTTGAAGCACCTGAAGGTTTTGGAGTTGGCAGTGATTTACATAATGATGTTTGTATTGAATCTGTACGAGTACACGAAGAAGAACTTGGGGCCTCTGAGATCGATTGTGTGGACTGTGGAGGTTGCTATGATCAATATTGTTGCTCCAATCTACTGGAGATTCGAAGATGCTATGATCAATATTGTTGCTCCAATCTACCGGAGATTCGAAGGAGTGCCTGATGATTTCCTTGGTTTACTCGACACGAAGGTTGAAGATATGAGATTGATAATCAGGGAGGAGGAGAAGAGAGGGAGGAGGAGAAAAGCAAGGCAATCAGGGAGAAAGAAGAAGAAGAAGAAGAGAAAAGAGGGAGCATGGGGAAGAGAAAGAAAATAA

Protein Analysis

140

Amino Acids

16.51

Weight (kDa)

10.33

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 11 - 71 1.2e-14 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 74
AfaI GTAC 2 cut(s) 107, 113
AgsI TTSAA 3 cut(s) 46, 100, 296
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 1 cut(s) 32
AluI AGCT 1 cut(s) 32
AoxI GGCC 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 131
AsuII TTCGAA 2 cut(s) 204, 249
BclI TGATCA 2 cut(s) 171, 216
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 132
BmsI GCATC 1 cut(s) 199
BpmI CTGGAG 1 cut(s) 217
Bpu14I TTCGAA 2 cut(s) 204, 249
Bsa29I ATCGAT 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 271
BsaWI WCCGGW 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 200
BseCI ATCGAT 1 cut(s) 143
BseDI CCNNGG 1 cut(s) 271
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 200
BseRI GAGGAG 4 cut(s) 335, 338, 352, 355
BseYI CCCAGC 1 cut(s) 17
BshFI GGCC 1 cut(s) 133
BshVI ATCGAT 1 cut(s) 143
BsiSI CCGG 1 cut(s) 241
BslFI GGGAC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 19
BsnI GGCC 1 cut(s) 133
Bsp119I TTCGAA 2 cut(s) 204, 249
Bsp143I GATC 3 cut(s) 140, 171, 216
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 1 cut(s) 129
BspDI ATCGAT 1 cut(s) 143
BspLI GGNNCC 1 cut(s) 132
BspT104I TTCGAA 2 cut(s) 204, 249
BsrI ACTGG 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 3 cut(s) 140, 171, 216
BssT1I CCWWGG 1 cut(s) 271
Bst4CI ACNGT 1 cut(s) 157
Bst6I CTCTTC 3 cut(s) 323, 377, 400
BstBI TTCGAA 2 cut(s) 204, 249
BstDEI CTNAG 2 cut(s) 33, 137
BstKTI GATC 3 cut(s) 143, 174, 219
BstMBI GATC 3 cut(s) 140, 171, 216
BstXI CCANNNNNNTGG 1 cut(s) 195
Bsu15I ATCGAT 1 cut(s) 143
BsuRI GGCC 1 cut(s) 133
BsuTUI ATCGAT 1 cut(s) 143
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 131
ClaI ATCGAT 1 cut(s) 143
Csp6I GTAC 2 cut(s) 106, 112
CviAII CATG 1 cut(s) 399
CviJI RGCY 3 cut(s) 7, 32, 133
CviKI_1 RGCY 3 cut(s) 7, 32, 133
CviQI GTAC 2 cut(s) 106, 112
DdeI CTNAG 2 cut(s) 33, 137
DpnI GATC 3 cut(s) 142, 173, 218
DpnII GATC 3 cut(s) 140, 171, 216
Eam1104I CTCTTC 3 cut(s) 323, 377, 400
EarI CTCTTC 3 cut(s) 323, 377, 400
Eco130I CCWWGG 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 1 cut(s) 402
FaiI YATR 6 cut(s) 11, 83, 170, 215, 302, 400
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 1 cut(s) 34
FatI CATG 1 cut(s) 398
FbaI TGATCA 2 cut(s) 171, 216
GsaI CCCAGC 1 cut(s) 21
GsuI CTGGAG 1 cut(s) 217
HaeIII GGCC 1 cut(s) 133
HapII CCGG 1 cut(s) 241
Hin1II CATG 1 cut(s) 402
HinfI GANTC 3 cut(s) 100, 201, 246
HpaII CCGG 1 cut(s) 241
Hpy166II GTNNAC 3 cut(s) 114, 153, 278
Hpy188I TCNGA 1 cut(s) 138
Hpy8I GTNNAC 3 cut(s) 114, 153, 278
HpyAV CCTTC 4 cut(s) 31, 49, 245, 283
HpyCH4III ACNGT 1 cut(s) 157
HpyF3I CTNAG 2 cut(s) 33, 137
Hsp92II CATG 1 cut(s) 402
Ksp22I TGATCA 2 cut(s) 171, 216
Kzo9I GATC 3 cut(s) 140, 171, 216
LmnI GCTCC 3 cut(s) 190, 235, 395
LpnPI CCDG 7 cut(s) 3, 65, 181, 254, 273, 301, 345
LweI GCATC 1 cut(s) 199
MalI GATC 3 cut(s) 142, 173, 218
MboI GATC 3 cut(s) 140, 171, 216
MnlI CCTC 8 cut(s) 144, 154, 313, 316, 326, 330, 333, 385
MslI CAYNNNNRTG 1 cut(s) 86
MspI CCGG 1 cut(s) 241
Mva1269I GAATGC 1 cut(s) 19
NdeII GATC 3 cut(s) 140, 171, 216
NlaIII CATG 1 cut(s) 402
NlaIV GGNNCC 1 cut(s) 132
NspV TTCGAA 2 cut(s) 204, 249
PctI GAATGC 1 cut(s) 19
PfeI GAWTC 3 cut(s) 100, 201, 246
PspFI CCCAGC 1 cut(s) 17
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 1 cut(s) 131
RsaI GTAC 2 cut(s) 107, 113
RsaNI GTAC 2 cut(s) 106, 112
RseI CAYNNNNRTG 1 cut(s) 86
Sau3AI GATC 3 cut(s) 140, 171, 216
Sau96I GGNCC 1 cut(s) 131
SetI ASST 5 cut(s) 34, 54, 60, 165, 294
SfaNI GCATC 1 cut(s) 199
SfuI TTCGAA 2 cut(s) 204, 249
SmiMI CAYNNNNRTG 1 cut(s) 86
SspI AATATT 2 cut(s) 178, 223
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 1 cut(s) 157
TaqI TCGA 4 cut(s) 143, 204, 249, 282
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 3 cut(s) 100, 201, 246
TscAI CASTG 1 cut(s) 78
TspDTI ATGAA 2 cut(s) 17, 26
TspRI CASTG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.