Rroxscaffold_4G00307070

Nsp1-like C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013006
Physical Location & Seq
Reverse (-)
28134575 .. 28140445
5871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00307070.1

Sequence Viewer

Length: 2187 bp
ATGTCGGGTTTCAATTTCGGATCCTCCTCGGCCTCCGCTTCACAATCCACTTCCCCTTTCGGATCCTCCACCCCTACTTTCTCATTCACCTCCTCCCCTTCCCTCTTCGGATCCTCCGCGCCCGCCACCGCCTCCACCGGGTTCGGACTCGGATCCTCCTCTTTCGGCTCCGCTCCCTCAAACCCTTCCACCTCCTCCTCCCCTTTCAGCTTCTCATCCAACACCGCCTCCTCCCCCGCTTTCGGTACCCCAAACCCTAGCCCCGCCCCGAACCCAAACCAGAACCCGTTTTCCTCATTCGGAGTCGGATCCTCCACCGTCTCAACCACCACCACCGCCGCAGCTTCTACACCTTCATTCGGATTCAATCCCTCCACTGCCGGTTCAGCTTCCACTCCGTCGCAGCCTATTTTCGGCTCAACCCCATCTCCGTCCCTGTTTGGCTCATCCTCATCGGCGCAGGCTCCGGGTCCTACCACATCCTTATTCGGGTCAACTGCATCTGCCTCCGCTTCCGGCACTACCAGCTCTCCTTTGTTCGGCTCGGCTTCGGCTTCGGCTTCACCTTTGTTTGGTATTACTACTACTACTTCTTCTTTCACTCCCAGCACAGGCTCGGCTCTCTTTGGCTCATCCTCTTCATCGCCGGCGCCGCCCGCCTCCACCACTCAGAACTTGTTCGGCTCTGCTCTGTCGTCCGCGGCTCCAACCACTCCCTCGTTCCCAAGTTTTTCAAGCTCTTCTTCTGCCGCCCCAACCACTCCCGCATTCTCAAGCTTTCTGAGCTCTTCCTCTGCAGCCTCAACCACTCCTGCATTCTCCAGCTTTTTGAGCTCTTCTTCTGCAGCTCCGGCCACTTCCTCGTTCGCAAGCTCTTCGGGTTTCTCATCATTCCCGAGTTCAGCTCCTGCTAAGCCCACAGCAGCATCCACACCTACTACTACAGCGCCTTCATCAAGCAGCAGTTTCAACCTCTCATTTGGAACGCCCACATCCTCGGCTTCTCAACCATCTTTCGGATTCAGCAATGCTGCCTCATCAGCCGTGGCGGCTTCCACCGCATTGTCATTTTCAAAGCCTTCATCGCCATTGTTTTCTACCGTCACGACCACCACGGCTTCTTCAACTCCGGCTGCAAGTAGTACTACACAAGCTCCTTTCTCTTTGTCGGCTTTCAATGTGACTCCTACTGCTCCTCCTGCTGCAAGCACCACTGCACTGACTTCAACGGCAGTGCCTGCCAGCTCAGCTGCTGCTTCATCAAGCACTACTACGAGTTCCTTTACAGGTTTCTCCATAACACCTGCTTCTTCGGCGGCTGCTGCTGCTTCTTCTTCTGGCCTTTCATTGTTTTCTAGTGCCTCTGTGCCCGCCTCGTCATCACAAGCACAACCAAGCGCCACTCTGCCCGCATTTGGTCTCACTACTTCAGCAACTGCAACTACAACGACGACTGGTACCAGTACCTCTGCAGCTCCGACATCAAGTGCACTTGCTGTGGCTTCTACTAGTGGGACAACCTCAAATGTTAATGGCAGCACTACAGTATCTACGACAACACCAAAATTGCCTTCTGAGATCACTGGGAAGACTGTTGAGGAGATTATCAAGGAGTGGAATGCTGAGCTTCAAGAGCGTACTGGAAAATTCCGAAAGCAGGCTAATGCAATAGCAGACTGGGACAGGAGGATTTTGCAGAACCGTGATGTTCTTCTTAGGCTCGAGATCGAAGTGGCAAATGTGGTAGATACCCAAGCTAAGGTGGAGCGGCAACTAGAGTTAATTGAGACTCATCAGCAAGAGGTCGATAAGGCTTTACAAAGCATGGAACAAGAAGCTGAGCGTATATACAAGGATGAGCGTGGATCACTTCTTGATGATGAAGCTGCATCCACAAGAGATGCAATGTATGAGCAGGCTGAGTTTATAGAGAGGGAGCTAGAGCAGGTGACAGAGCAAATCAAATCAATTATTGAGACTGTAAATGCCAACCAGGGAGGAGAATTGGAAGTAAATGATGGGATGGCTCCACTAGATGTGGTTGTACGAATCTTAAACAACCAACTAAGCTCCTTAATGTGGATTGATGAAAAGGCCGAAGAGTTCTCTTCGCGAATTCAGAAGCTTGCCAATCAACGTCCTGCTGCAGATCGTGAATTGATGGGTCCAAGATACTGGATGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0003674 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005642 GO:0005643 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0008092 GO:0008104 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008285 GO:0008289 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010648 GO:0010928 GO:0010930 GO:0010941 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016043 GO:0016070 GO:0017038 GO:0017056 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019894 GO:0019904 GO:0019953 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030159 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032182 GO:0032386 GO:0032501 GO:0032504 GO:0032774 GO:0032879 GO:0032880 GO:0032947 GO:0032991 GO:0033036 GO:0033157 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0042127 GO:0042169 GO:0042175 GO:0042306 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043123 GO:0043130 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044613 GO:0044703 GO:0045184 GO:0045893 GO:0045935 GO:0046483 GO:0046822 GO:0046907 GO:0046966 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051223 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051259 GO:0051291 GO:0051427 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0060255 GO:0060341 GO:0060548 GO:0065003 GO:0065007 GO:0070013 GO:0070201 GO:0070206 GO:0070208 GO:0070727 GO:0071166 GO:0071426 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0098589 GO:0098805 GO:1900180 GO:1901360 GO:1901362 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1903827 GO:1904589 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nsp1_C Nucleoporin_FG

Protein Analysis

728

Amino Acids

73.08

Weight (kDa)

4.71

Isoelectric Point (pI)

58.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nsp1_C PF05064 518 - 621 6.4e-22 Nsp1-like C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45000
fragaria_vesca FvH4_7g10470 FvH4_7g10470 FvH4_7g10470
malus_domestica MD07G1110700.v1.1
prunus_persica Prupe.2G134200_v2.0.a1 Prupe.2G134200_v2.0.a1
pyrus_communis pycom07g09560
rosa_chinensis RchiOBHm_Chr1g0348261
rosa_laevigata RLG00000028672 RLG00000028676
rosa_roxburghii Rroxscaffold_4G00307070
rosa_rugosa Rorug01G0194000
rosa_samantha Rh1AG211000 Rh1BG177500 Rh1CG195400 Rh1DG207000
rosa_wichuraiana Rw1G017820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1312, 1936
Acc36I ACCTGC 2 cut(s) 1312, 1936
Acc65I GGTACC 2 cut(s) 245, 1457
AccB1I GGYRCC 3 cut(s) 245, 649, 1457
AccBSI CCGCTC 2 cut(s) 173, 1768
AccII CGCG 3 cut(s) 119, 701, 2113
AcoI YGGCCR 1 cut(s) 852
AcsI RAATTY 2 cut(s) 1646, 2115
AcuI CTGAAG 1 cut(s) 1413
AcyI GRCGYC 1 cut(s) 650
AfaI GTAC 6 cut(s) 247, 1144, 1459, 1465, 1639, 2046
AgsI TTSAA 9 cut(s) 13, 367, 735, 970, 1074, 1125, 1177, 1227, 1631
AhlI ACTAGT 1 cut(s) 1508
AjnI CCWGG 1 cut(s) 1992
AjuI GAANNNNNNNTTGG 2 cut(s) 1555, 1587
AloI GAACNNNNNNTCC 2 cut(s) 275, 307
Alw21I GWGCWC 3 cut(s) 788, 836, 1492
Alw26I GTCTC 4 cut(s) 325, 1424, 1781, 1970
Alw44I GTGCAC 1 cut(s) 1488
AlwNI CAGNNNCTG 4 cut(s) 908, 1238, 1253, 1436
Ama87I CYCGRG 2 cut(s) 895, 1721
AoxI GGCC 4 cut(s) 30, 852, 1339, 2094
ApaLI GTGCAC 1 cut(s) 1488
ApoI RAATTY 2 cut(s) 1646, 2115
Asp700I GAANNNNTTC 1 cut(s) 677
Asp718I GGTACC 2 cut(s) 245, 1457
AspLEI GCGC 5 cut(s) 121, 460, 652, 949, 1400
AspS9I GGNCC 2 cut(s) 470, 2165
AsuC2I CCSGG 2 cut(s) 139, 468
AsuHPI GGTGA 3 cut(s) 79, 555, 1960
AvaI CYCGRG 2 cut(s) 895, 1721
AvaII GGWCC 2 cut(s) 470, 2165
BaeGI GKGCMC 2 cut(s) 1371, 1492
BaeI ACNNNNGTAYC 2 cut(s) 1530, 1563
BamHI GGATCC 5 cut(s) 20, 62, 110, 152, 308
BanI GGYRCC 3 cut(s) 245, 649, 1457
BanII GRGCYC 2 cut(s) 788, 836
BbsI GAAGAC 1 cut(s) 1595
Bbv12I GWGCWC 3 cut(s) 788, 836, 1492
BccI CCATC 5 cut(s) 433, 1018, 2012, 2017, 2155
BceAI ACGGC 3 cut(s) 1028, 1131, 1245
BcgI CGANNNNNNTGC 2 cut(s) 858, 892
BciT130I CCWGG 1 cut(s) 1994
BcnI CCSGG 2 cut(s) 139, 468
BcoDI GTCTC 4 cut(s) 325, 1424, 1781, 1970
BcuI ACTAGT 1 cut(s) 1508
BfaI CTAG 6 cut(s) 258, 1356, 1509, 1775, 1940, 2033
BfmI CTRYAG 6 cut(s) 795, 843, 942, 1470, 1542, 2145
BfoI RGCGCY 3 cut(s) 653, 950, 1401
BfuAI ACCTGC 2 cut(s) 1312, 1936
BglI GCCNNNNNGGC 1 cut(s) 1049
BlpI GCTNAGC 4 cut(s) 912, 1246, 1623, 1839
BmcAI AGTACT 1 cut(s) 1144
Bme1390I CCNGG 3 cut(s) 139, 468, 1994
Bme18I GGWCC 2 cut(s) 470, 2165
BmeT110I CYCGRG 2 cut(s) 895, 1721
BmgT120I GGNCC 2 cut(s) 470, 2165
BmrFI CCNGG 3 cut(s) 139, 468, 1994
BmrI ACTGGG 2 cut(s) 1593, 1687
BmsI GCATC 4 cut(s) 509, 935, 1891, 1898
BmuI ACTGGG 2 cut(s) 1593, 1687
BpiI GAAGAC 1 cut(s) 1595
BplI GAGNNNNNCTC 2 cut(s) 889, 921
BpmI CTGGAG 1 cut(s) 805
Bpu10I CCTNAGC 1 cut(s) 1758
Bpu1102I GCTNAGC 4 cut(s) 912, 1246, 1623, 1839
BpuEI CTTGAG 1 cut(s) 757
BpuMI CCSGG 2 cut(s) 139, 468
BsaHI GRCGYC 1 cut(s) 650
BsaI GGTCTC 1 cut(s) 1424
BsaJI CCNNGG 6 cut(s) 27, 699, 996, 1044, 1113, 1993
Bse118I RCCGGY 2 cut(s) 380, 646
Bse1I ACTGG 6 cut(s) 1459, 1461, 1588, 1645, 1682, 2180
Bse3DI GCAATG 2 cut(s) 1033, 1911
BseBI CCWGG 1 cut(s) 1994
BseDI CCNNGG 6 cut(s) 27, 699, 996, 1044, 1113, 1993
BseMI GCAATG 2 cut(s) 1033, 1911
BseMII CTCAG 7 cut(s) 683, 773, 1260, 1566, 1614, 1830, 1911
BseNI ACTGG 6 cut(s) 1459, 1461, 1588, 1645, 1682, 2180
BseRI GAGGAG 9 cut(s) 16, 82, 148, 184, 187, 220, 1185, 1613, 2013
BseSI GKGCMC 2 cut(s) 1371, 1492
BseYI CCCAGC 1 cut(s) 605
BsgI GTGCAG 1 cut(s) 1200
Bsh1236I CGCG 3 cut(s) 119, 701, 2113
BshFI GGCC 4 cut(s) 32, 854, 1341, 2096
BshNI GGYRCC 3 cut(s) 245, 649, 1457
BsiHKAI GWGCWC 3 cut(s) 788, 836, 1492
BsiHKCI CYCGRG 2 cut(s) 895, 1721
BsiSI CCGG 7 cut(s) 138, 381, 467, 516, 647, 851, 1130
BslFI GGGAC 3 cut(s) 418, 1528, 1694
BsmAI GTCTC 4 cut(s) 325, 1424, 1781, 1970
BsmBI CGTCTC 1 cut(s) 325
BsmFI GGGAC 3 cut(s) 418, 1528, 1694
BsmI GAATGC 3 cut(s) 767, 815, 1624
BsnI GGCC 4 cut(s) 32, 854, 1341, 2096
Bso31I GGTCTC 1 cut(s) 1424
BsoBI CYCGRG 2 cut(s) 895, 1721
Bsp1286I GDGCHC 4 cut(s) 788, 836, 1371, 1492
Bsp143I GATC 9 cut(s) 20, 62, 110, 152, 308, 1578, 1725, 1865, 2149
Bsp1720I GCTNAGC 4 cut(s) 912, 1246, 1623, 1839
Bsp68I TCGCGA 1 cut(s) 2113
BspANI GGCC 4 cut(s) 32, 854, 1341, 2096
BspCNI CTCAG 7 cut(s) 682, 774, 1259, 1567, 1615, 1831, 1912
BspFNI CGCG 3 cut(s) 119, 701, 2113
BspHI TCATGA 1 cut(s) 2183
BspMAI CTGCAG 4 cut(s) 799, 847, 1474, 2149
BspMI ACCTGC 2 cut(s) 1312, 1936
BspQI GCTCTTC 4 cut(s) 745, 793, 841, 880
BspT107I GGYRCC 3 cut(s) 245, 649, 1457
BspTNI GGTCTC 1 cut(s) 1424
BsrBI CCGCTC 2 cut(s) 173, 1768
BsrDI GCAATG 2 cut(s) 1033, 1911
BsrFI RCCGGY 2 cut(s) 380, 646
BsrI ACTGG 6 cut(s) 1459, 1461, 1588, 1645, 1682, 2180
BssAI RCCGGY 2 cut(s) 380, 646
BssECI CCNNGG 6 cut(s) 27, 699, 996, 1044, 1113, 1993
BssMI GATC 9 cut(s) 20, 62, 110, 152, 308, 1578, 1725, 1865, 2149
BssNI GRCGYC 1 cut(s) 650
Bst2UI CCWGG 1 cut(s) 1994
Bst4CI ACNGT 6 cut(s) 319, 1102, 1546, 1594, 1703, 1981
Bst6I CTCTTC 8 cut(s) 110, 643, 745, 793, 841, 880, 2094, 2113
BstACI GRCGYC 1 cut(s) 650
BstAPI GCANNNNNTGC 1 cut(s) 1238
BstDSI CCRYGG 3 cut(s) 699, 1044, 1113
BstFNI CGCG 3 cut(s) 119, 701, 2113
BstH2I RGCGCY 3 cut(s) 653, 950, 1401
BstHHI GCGC 5 cut(s) 121, 460, 652, 949, 1400
BstKTI GATC 9 cut(s) 23, 65, 113, 155, 311, 1581, 1728, 1868, 2152
BstMAI GTCTC 4 cut(s) 325, 1424, 1781, 1970
BstMBI GATC 9 cut(s) 20, 62, 110, 152, 308, 1578, 1725, 1865, 2149
BstNI CCWGG 1 cut(s) 1994
BstSCI CCNGG 3 cut(s) 137, 466, 1992
BstSFI CTRYAG 6 cut(s) 795, 843, 942, 1470, 1542, 2145
BstSLI GKGCMC 2 cut(s) 1371, 1492
BstUI CGCG 3 cut(s) 119, 701, 2113
BstV2I GAAGAC 1 cut(s) 1595
BstX2I RGATCY 5 cut(s) 20, 62, 110, 152, 308
BstXI CCANNNNNNTGG 1 cut(s) 2175
BstYI RGATCY 5 cut(s) 20, 62, 110, 152, 308
BsuRI GGCC 4 cut(s) 32, 854, 1341, 2096
BtgI CCRYGG 3 cut(s) 699, 1044, 1113
BtgZI GCGATG 2 cut(s) 627, 1068
BtsI GCAGTG 3 cut(s) 375, 1212, 1239
BtsIMutI CAGTG 5 cut(s) 375, 1212, 1217, 1239, 1581
BtuMI TCGCGA 1 cut(s) 2113
BveI ACCTGC 2 cut(s) 1312, 1936
CaiI CAGNNNCTG 4 cut(s) 908, 1238, 1253, 1436
CciI TCATGA 1 cut(s) 2183
CfoI GCGC 5 cut(s) 121, 460, 652, 949, 1400
Cfr10I RCCGGY 2 cut(s) 380, 646
Cfr13I GGNCC 2 cut(s) 470, 2165
Cfr42I CCGCGG 1 cut(s) 702
Csp6I GTAC 6 cut(s) 246, 1143, 1458, 1464, 1638, 2045
CviAII CATG 2 cut(s) 1825, 2184
CviQI GTAC 6 cut(s) 246, 1143, 1458, 1464, 1638, 2045
DinI GGCGCC 1 cut(s) 651
DpnI GATC 9 cut(s) 22, 64, 112, 154, 310, 1580, 1727, 1867, 2151
DpnII GATC 9 cut(s) 20, 62, 110, 152, 308, 1578, 1725, 1865, 2149
EaeI YGGCCR 1 cut(s) 852
Eam1104I CTCTTC 8 cut(s) 110, 643, 745, 793, 841, 880, 2094, 2113
EarI CTCTTC 8 cut(s) 110, 643, 745, 793, 841, 880, 2094, 2113
Ecl136II GAGCTC 2 cut(s) 786, 834
Eco24I GRGCYC 2 cut(s) 788, 836
Eco31I GGTCTC 1 cut(s) 1424
Eco47I GGWCC 2 cut(s) 470, 2165
Eco53kI GAGCTC 2 cut(s) 786, 834
Eco57I CTGAAG 1 cut(s) 1413
Eco88I CYCGRG 2 cut(s) 895, 1721
EcoICRI GAGCTC 2 cut(s) 786, 834
EcoO109I RGGNCCY 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 2115
EcoRII CCWGG 1 cut(s) 1992
EcoT38I GRGCYC 2 cut(s) 788, 836
EgeI GGCGCC 1 cut(s) 651
EheI GGCGCC 1 cut(s) 651
Esp3I CGTCTC 1 cut(s) 325
FaeI CATG 2 cut(s) 1828, 2187
FaiI YATR 7 cut(s) 1298, 1826, 1847, 1849, 1911, 1928, 2185
FalI AAGNNNNNCTT 2 cut(s) 727, 759
FaqI GGGAC 3 cut(s) 418, 1528, 1694
FatI CATG 2 cut(s) 1824, 2183
FauI CCCGC 7 cut(s) 130, 244, 271, 664, 772, 1378, 1417
FokI GGATG 9 cut(s) 202, 433, 466, 619, 913, 979, 1868, 1876, 2035
FriOI GRGCYC 2 cut(s) 788, 836
FspBI CTAG 6 cut(s) 258, 1356, 1509, 1775, 1940, 2033
GlaI GCGC 5 cut(s) 120, 459, 651, 948, 1399
GsaI CCCAGC 1 cut(s) 609
GsuI CTGGAG 1 cut(s) 805
HaeII RGCGCY 3 cut(s) 653, 950, 1401
HaeIII GGCC 4 cut(s) 32, 854, 1341, 2096
HapII CCGG 7 cut(s) 138, 381, 467, 516, 647, 851, 1130
HhaI GCGC 5 cut(s) 121, 460, 652, 949, 1400
Hin1I GRCGYC 1 cut(s) 650
Hin1II CATG 2 cut(s) 1828, 2187
Hin6I GCGC 5 cut(s) 119, 458, 650, 947, 1398
HinP1I GCGC 5 cut(s) 119, 458, 650, 947, 1398
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HindIII AAGCTT 2 cut(s) 775, 2123
HinfI GANTC 7 cut(s) 147, 303, 363, 1020, 1183, 1789, 2049
HpaII CCGG 7 cut(s) 138, 381, 467, 516, 647, 851, 1130
HphI GGTGA 3 cut(s) 79, 555, 1960
Hpy166II GTNNAC 2 cut(s) 495, 1490
Hpy188III TCNNGA 8 cut(s) 895, 1105, 1631, 1723, 1874, 2112, 2153, 2184
Hpy8I GTNNAC 2 cut(s) 495, 1490
Hpy99I CGWCG 2 cut(s) 403, 1453
HpyAV CCTTC 6 cut(s) 108, 195, 363, 960, 1089, 1581
HpyCH4III ACNGT 6 cut(s) 319, 1102, 1546, 1594, 1703, 1981
HpyCH4IV ACGT 1 cut(s) 2137
HpySE526I ACGT 1 cut(s) 2137
Hsp92I GRCGYC 1 cut(s) 650
Hsp92II CATG 2 cut(s) 1828, 2187
HspAI GCGC 5 cut(s) 119, 458, 650, 947, 1398
KasI GGCGCC 1 cut(s) 649
KpnI GGTACC 2 cut(s) 249, 1461
KroI GCCGGC 1 cut(s) 646
KroNI GCCGGC 1 cut(s) 648
KspI CCGCGG 1 cut(s) 702
Kzo9I GATC 9 cut(s) 20, 62, 110, 152, 308, 1578, 1725, 1865, 2149
LguI GCTCTTC 4 cut(s) 745, 793, 841, 880
LweI GCATC 4 cut(s) 509, 935, 1891, 1898
MaeI CTAG 6 cut(s) 258, 1356, 1509, 1775, 1940, 2033
MaeII ACGT 1 cut(s) 2137
MaeIII GTNAC 3 cut(s) 1102, 1180, 1948
MalI GATC 9 cut(s) 22, 64, 112, 154, 310, 1580, 1727, 1867, 2151
MbiI CCGCTC 2 cut(s) 173, 1768
MboI GATC 9 cut(s) 20, 62, 110, 152, 308, 1578, 1725, 1865, 2149
MflI RGATCY 5 cut(s) 20, 62, 110, 152, 308
MhlI GDGCHC 4 cut(s) 788, 836, 1371, 1492
MluCI AATT 8 cut(s) 13, 1565, 1646, 1782, 1968, 2003, 2115, 2156
Mly113I GGCGCC 1 cut(s) 650
MlyI GAGTC 4 cut(s) 141, 312, 1177, 1783
MmeI TCCRAC 4 cut(s) 243, 286, 731, 1502
MreI CGCCGGCG 1 cut(s) 646
MroNI GCCGGC 1 cut(s) 646
MroXI GAANNNNTTC 1 cut(s) 677
MseI TTAA 4 cut(s) 1530, 1781, 2054, 2075
MspA1I CMGCKG 2 cut(s) 701, 1250
MspI CCGG 7 cut(s) 138, 381, 467, 516, 647, 851, 1130
MspR9I CCNGG 3 cut(s) 139, 468, 1994
Mva1269I GAATGC 3 cut(s) 767, 815, 1624
MvaI CCWGG 1 cut(s) 1994
MvnI CGCG 3 cut(s) 119, 701, 2113
NaeI GCCGGC 1 cut(s) 648
NarI GGCGCC 1 cut(s) 650
NciI CCSGG 2 cut(s) 139, 468
NdeII GATC 9 cut(s) 20, 62, 110, 152, 308, 1578, 1725, 1865, 2149
NgoMIV GCCGGC 1 cut(s) 646
NlaIII CATG 2 cut(s) 1828, 2187
NmeAIII GCCGAG 4 cut(s) 8, 524, 596, 977
NmuCI GTSAC 3 cut(s) 1102, 1180, 1948
NruI TCGCGA 1 cut(s) 2113
PaeR7I CTCGAG 1 cut(s) 1721
PagI TCATGA 1 cut(s) 2183
PaqCI CACCTGC 2 cut(s) 1312, 1936
PciSI GCTCTTC 4 cut(s) 745, 793, 841, 880
PctI GAATGC 3 cut(s) 767, 815, 1624
PdiI GCCGGC 1 cut(s) 648
PdmI GAANNNNTTC 1 cut(s) 677
PfeI GAWTC 3 cut(s) 363, 1020, 2049
PleI GAGTC 4 cut(s) 141, 311, 1177, 1783
PluTI GGCGCC 1 cut(s) 653
PpsI GAGTC 4 cut(s) 141, 311, 1177, 1783
PpuMI RGGWCCY 1 cut(s) 470
Psp124BI GAGCTC 2 cut(s) 788, 836
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 1992
PspFI CCCAGC 1 cut(s) 605
PspGI CCWGG 1 cut(s) 1992
PspPI GGNCC 2 cut(s) 470, 2165
PspPPI RGGWCCY 1 cut(s) 470
PstI CTGCAG 4 cut(s) 799, 847, 1474, 2149
PstNI CAGNNNCTG 4 cut(s) 908, 1238, 1253, 1436
PsuI RGATCY 5 cut(s) 20, 62, 110, 152, 308
PvuII CAGCTG 1 cut(s) 1250
RruI TCGCGA 1 cut(s) 2113
RsaI GTAC 6 cut(s) 247, 1144, 1459, 1465, 1639, 2046
RsaNI GTAC 6 cut(s) 246, 1143, 1458, 1464, 1638, 2045
SacI GAGCTC 2 cut(s) 788, 836
SacII CCGCGG 1 cut(s) 702
SapI GCTCTTC 4 cut(s) 745, 793, 841, 880
SaqAI TTAA 4 cut(s) 1530, 1781, 2054, 2075
Sau3AI GATC 9 cut(s) 20, 62, 110, 152, 308, 1578, 1725, 1865, 2149
Sau96I GGNCC 2 cut(s) 470, 2165
ScaI AGTACT 1 cut(s) 1144
SchI GAGTC 4 cut(s) 141, 312, 1177, 1783
ScrFI CCNGG 3 cut(s) 139, 468, 1994
SduI GDGCHC 4 cut(s) 788, 836, 1371, 1492
SfaNI GCATC 4 cut(s) 509, 935, 1891, 1898
SfcI CTRYAG 6 cut(s) 795, 843, 942, 1470, 1542, 2145
SfoI GGCGCC 1 cut(s) 651
Sfr274I CTCGAG 1 cut(s) 1721
Sfr303I CCGCGG 1 cut(s) 702
SgrAI CRCCGGYG 1 cut(s) 646
SgrBI CCGCGG 1 cut(s) 702
SinI GGWCC 2 cut(s) 470, 2165
SlaI CTCGAG 1 cut(s) 1721
SmlI CTYRAG 2 cut(s) 772, 1721
SmoI CTYRAG 2 cut(s) 772, 1721
SpeI ACTAGT 1 cut(s) 1508
Sse9I AATT 8 cut(s) 13, 1565, 1646, 1782, 1968, 2003, 2115, 2156
SspDI GGCGCC 1 cut(s) 649
SspMI CTAG 6 cut(s) 258, 1356, 1509, 1775, 1940, 2033
SstI GAGCTC 2 cut(s) 788, 836
StyD4I CCNGG 3 cut(s) 137, 466, 1992
TaaI ACNGT 6 cut(s) 319, 1102, 1546, 1594, 1703, 1981
TaiI ACGT 1 cut(s) 2140
TaqI TCGA 3 cut(s) 1722, 1728, 1806
TasI AATT 8 cut(s) 13, 1565, 1646, 1782, 1968, 2003, 2115, 2156
TatI WGTACW 1 cut(s) 1142
TauI GCSGC 7 cut(s) 341, 655, 704, 752, 1052, 1319, 1771
TfiI GAWTC 3 cut(s) 363, 1020, 2049
Tru1I TTAA 4 cut(s) 1530, 1781, 2054, 2075
Tru9I TTAA 4 cut(s) 1530, 1781, 2054, 2075
TscAI CASTG 5 cut(s) 382, 1219, 1224, 1239, 1588
TseFI GTSAC 3 cut(s) 1102, 1180, 1948
Tsp45I GTSAC 3 cut(s) 1102, 1180, 1948
TspDTI ATGAA 8 cut(s) 345, 630, 942, 1071, 1248, 1335, 1896, 2103
TspGWI ACGGA 2 cut(s) 387, 420
TspRI CASTG 5 cut(s) 382, 1219, 1224, 1239, 1588
VneI GTGCAC 1 cut(s) 1488
VpaK11BI GGWCC 2 cut(s) 470, 2165
XapI RAATTY 2 cut(s) 1646, 2115
XhoI CTCGAG 1 cut(s) 1721
XmnI GAANNNNTTC 1 cut(s) 677
XspI CTAG 6 cut(s) 258, 1356, 1509, 1775, 1940, 2033
ZrmI AGTACT 1 cut(s) 1144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.