Rh1AG211000

Nsp1-like C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0013006
Physical Location & Seq
Forward (+)
40399431 .. 40405336
5906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG211000.1

Sequence Viewer

Length: 1932 bp
ATGTCGGGTTTCAATTTCGGATCCTCCGCGGCCTCCGCTTCACAATCCACTTCCCCTTTCGGATCCTCCACCCCGGCTTTCTCATTCGCCTCCTCCCCTTCCCTCTTCGGATCCTCCGCGCCCGCCACCGCCTCCACCGGGTTCGGAGTCGGATCCTCCTCTTTCGGCTCCGCTCCCTCAAACCCTTCCACCTCCTCCTCCCCTTTCAGCTTCTCATCCAACACCGCCTCCTCCCCCGCTTTCGGTACCCCAAACCAGAACCCGTTTCCCTCATTCGGAGTCGGATCCTCCACCGCCTCAACCACCACCACCGCCGCAGCTTCCTCACCTTTCGGATTCAATCCCTCAACGGCCGGTTCAGGCTCCACTCCGTCGCAGCCTATGTTCGGCTCAACCGCACTCCAATCCCTGTTTGGCTCATCCTCATCGGCGCAGGCTCCGGGTTCTACCCCATCCTTATTCGGGTCAACTGCATCTGCCTCCGCTTCCGGCACTACCAGCTCTCCTCTGTTCGGTTCGGCTTCGGCTTCACCTCTGTTTGGTACTACTACTACTACTTCTTTCGCTCCCAGCACAGGCTCGGCTCTCTTTGGCTCGTCCTCTTCATCGGCGGCGCCGCCTTCCTCCACCACTCAGAACTTGTTTGGCTCTGCTCCGTCGTCCGCGGCTCCAACCACTCCCTCATTCCAAAGCTTTTCGAGCTCTTCCTCTGCAGCCCCAACCACTCCTGCATTCTCCAGCTTTTTGGGCTCTTCGTCTGCAGCATCAACCACTCCCACTCCCGCATTCCCATCCTTTTCGAGCTCTTCCGCTGCAGCCTCAACCACTTCTCCTTTCGCAGGCTTTTCGAGCTCTTCTTCTGCAGCTCCGGCCACTACAAGCTCTTCGGGGTTCTCACCATTCCCGAGTTCAGCTCCTGCTAAGCCCACAGCAGCATCCACACCTACTACTACGGCGCCTTCATCAAGCAGCGGTTTCAACCTCTCATTTGGAACGCCCACATCCTCGGCTTCTCAACCTTCTTTTGGATTCAGCAATGCCGCCTCATCAGCCGCAGCGGCTTCCGCCGCATTGTCATTTTCAAAGTCTTCATCGCCATTGTTTTCAACCGTCACGACCACCACGGCTTCTTCAACTCCGGCTGCAAGTAGTACTACACAAGCTCCTTTCTCTTTGCCGGCTTTCAATGTGACTCCTACTGCTCCTCCTGCTGCGAGCACCACTGCACTGACTTCAACGGCAGTGCCTGCCAGCTCAGCTGCTGCTTCATCAAGCACTACTACGAGTTCCTTTACAGGTTTCTCCTTAACACCTGCTTCTTCGGCGGCTACTGCTTCTTCTTCTTCTGGCCTTTCATTGTTTTCCAGTGCCTCTGTGCCCGCCTCATCATCACAAGCACAACCAAGCACCACTCTGCCTGCATTTGGTCTCACTACTTCAGCAACTGCAACTACCACTGCAACTACAACGACGACTGGTACCAGTACCCCTGCTGCTCCGACATCAAGTGCACTTGCTGTGGCTTCTACTAGTGGGACAACCTCAAATGTTAATGGCAGCACTACAGTATCTACAACAACACCAAAATTACCTTCTGAGATCACTGGGAAGACTGTTGAGGAGATTATCAAGGAGTGGAATGCTGAGCTTCAAGAGCGTACTGGAAAATTCCGAAAGCAGGCTAATGCAATAGCAGACTGGGACAGGAGGATTTTGCAGAACCGTGATGTTCTTCTTAGGCTCGAGATCGAAGTGGCAAATGTGGTAGATACCCAAGCTAAGGTGGAGCGGCAACTAGAGTTAATTGAGACTCATCAGCAAGAGGTCGATAAGGCTTTACAAAGCATGGAACAGGAAGCTGAGCGTATATACAAGGATGAGCGTGGATCGCTTCTTGATGATGAAGCTGCATCCACAAGAGATGCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0003674 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005642 GO:0005643 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0008092 GO:0008104 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008285 GO:0008289 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010648 GO:0010928 GO:0010930 GO:0010941 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016043 GO:0016070 GO:0017038 GO:0017056 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019894 GO:0019904 GO:0019953 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030159 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032182 GO:0032386 GO:0032501 GO:0032504 GO:0032774 GO:0032879 GO:0032880 GO:0032947 GO:0032991 GO:0033036 GO:0033157 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0042127 GO:0042169 GO:0042175 GO:0042306 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043123 GO:0043130 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044613 GO:0044703 GO:0045184 GO:0045893 GO:0045935 GO:0046483 GO:0046822 GO:0046907 GO:0046966 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051223 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051259 GO:0051291 GO:0051427 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0060255 GO:0060341 GO:0060548 GO:0065003 GO:0065007 GO:0070013 GO:0070201 GO:0070206 GO:0070208 GO:0070727 GO:0071166 GO:0071426 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0098589 GO:0098805 GO:1900180 GO:1901360 GO:1901362 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1903827 GO:1904589 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nsp1_C Nucleoporin_FG

Protein Analysis

643

Amino Acids

62.95

Weight (kDa)

4.95

Isoelectric Point (pI)

62.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nsp1_C PF05064 525 - 628 5.3e-22 Nsp1-like C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45000
fragaria_vesca FvH4_7g10470 FvH4_7g10470 FvH4_7g10470
malus_domestica MD07G1110700.v1.1
prunus_persica Prupe.2G134200_v2.0.a1 Prupe.2G134200_v2.0.a1
pyrus_communis pycom07g09560
rosa_chinensis RchiOBHm_Chr1g0348261
rosa_laevigata RLG00000028672 RLG00000028676
rosa_roxburghii Rroxscaffold_4G00307070
rosa_rugosa Rorug01G0194000
rosa_samantha Rh1AG211000 Rh1BG177500 Rh1CG195400 Rh1DG207000
rosa_wichuraiana Rw1G017820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1321
Acc36I ACCTGC 1 cut(s) 1321
Acc65I GGTACC 2 cut(s) 245, 1478
AccB1I GGYRCC 4 cut(s) 245, 613, 955, 1478
AccBSI CCGCTC 2 cut(s) 173, 1789
AccII CGCG 3 cut(s) 29, 119, 665
AcoI YGGCCR 2 cut(s) 351, 870
AcsI RAATTY 1 cut(s) 1667
AcuI CTGAAG 1 cut(s) 1422
AcyI GRCGYC 2 cut(s) 614, 956
AfaI GTAC 6 cut(s) 247, 544, 1153, 1480, 1486, 1660
AgsI TTSAA 9 cut(s) 13, 340, 979, 1083, 1107, 1134, 1186, 1236, 1652
AhlI ACTAGT 1 cut(s) 1529
AjuI GAANNNNNNNTTGG 2 cut(s) 1576, 1608
Alw21I GWGCWC 5 cut(s) 704, 806, 854, 1220, 1513
Alw26I GTCTC 2 cut(s) 1433, 1802
Alw44I GTGCAC 1 cut(s) 1509
AlwNI CAGNNNCTG 4 cut(s) 917, 1247, 1262, 1445
Ama87I CYCGRG 2 cut(s) 904, 1742
AoxI GGCC 4 cut(s) 30, 351, 870, 1348
ApaLI GTGCAC 1 cut(s) 1509
ApoI RAATTY 1 cut(s) 1667
Asp718I GGTACC 2 cut(s) 245, 1478
AspLEI GCGC 4 cut(s) 121, 433, 616, 958
AsuC2I CCSGG 3 cut(s) 74, 139, 441
AsuHPI GGTGA 3 cut(s) 318, 522, 888
AvaI CYCGRG 2 cut(s) 904, 1742
BaeGI GKGCMC 2 cut(s) 1380, 1513
BaeI ACNNNNGTAYC 2 cut(s) 1551, 1584
BamHI GGATCC 5 cut(s) 20, 62, 110, 152, 284
BanI GGYRCC 4 cut(s) 245, 613, 955, 1478
BanII GRGCYC 4 cut(s) 704, 752, 806, 854
BbsI GAAGAC 2 cut(s) 1080, 1616
Bbv12I GWGCWC 5 cut(s) 704, 806, 854, 1220, 1513
BccI CCATC 2 cut(s) 460, 799
BceAI ACGGC 4 cut(s) 366, 969, 1140, 1254
BcgI CGANNNNNNTGC 2 cut(s) 828, 862
BcnI CCSGG 3 cut(s) 74, 139, 441
BcoDI GTCTC 2 cut(s) 1433, 1802
BcuI ACTAGT 1 cut(s) 1529
BfaI CTAG 2 cut(s) 1530, 1796
BfmI CTRYAG 5 cut(s) 711, 759, 813, 861, 1563
BfoI RGCGCY 2 cut(s) 617, 959
BfuAI ACCTGC 1 cut(s) 1321
BglI GCCNNNNNGGC 1 cut(s) 1058
BlpI GCTNAGC 4 cut(s) 921, 1255, 1644, 1860
BmcAI AGTACT 1 cut(s) 1153
Bme1390I CCNGG 3 cut(s) 74, 139, 441
BmeT110I CYCGRG 2 cut(s) 904, 1742
BmrFI CCNGG 3 cut(s) 74, 139, 441
BmrI ACTGGG 2 cut(s) 1614, 1708
BmsI GCATC 5 cut(s) 482, 773, 944, 1912, 1919
BmuI ACTGGG 2 cut(s) 1614, 1708
BpiI GAAGAC 2 cut(s) 1080, 1616
BplI GAGNNNNNCTC 2 cut(s) 898, 930
BpmI CTGGAG 1 cut(s) 721
Bpu10I CCTNAGC 1 cut(s) 1779
Bpu1102I GCTNAGC 4 cut(s) 921, 1255, 1644, 1860
BpuMI CCSGG 3 cut(s) 74, 139, 441
BsaHI GRCGYC 2 cut(s) 614, 956
BsaI GGTCTC 1 cut(s) 1433
BsaJI CCNNGG 5 cut(s) 27, 72, 663, 1005, 1122
Bse118I RCCGGY 2 cut(s) 353, 1177
Bse1I ACTGG 6 cut(s) 1365, 1480, 1482, 1609, 1666, 1703
Bse3DI GCAATG 2 cut(s) 1042, 1932
BseDI CCNNGG 5 cut(s) 27, 72, 663, 1005, 1122
BseGI GGATG 8 cut(s) 215, 419, 452, 791, 935, 1001, 1882, 1910
BseMI GCAATG 2 cut(s) 1042, 1932
BseMII CTCAG 5 cut(s) 647, 1269, 1587, 1635, 1851
BseNI ACTGG 6 cut(s) 1365, 1480, 1482, 1609, 1666, 1703
BseRI GAGGAG 8 cut(s) 82, 148, 184, 187, 220, 495, 1194, 1634
BseSI GKGCMC 2 cut(s) 1380, 1513
BseX3I CGGCCG 1 cut(s) 351
BseYI CCCAGC 1 cut(s) 569
BsgI GTGCAG 1 cut(s) 1209
Bsh1236I CGCG 3 cut(s) 29, 119, 665
Bsh1285I CGRYCG 1 cut(s) 354
BshFI GGCC 4 cut(s) 32, 353, 872, 1350
BshNI GGYRCC 4 cut(s) 245, 613, 955, 1478
BsiEI CGRYCG 1 cut(s) 354
BsiHKAI GWGCWC 5 cut(s) 704, 806, 854, 1220, 1513
BsiHKCI CYCGRG 2 cut(s) 904, 1742
BsiSI CCGG 8 cut(s) 74, 138, 354, 440, 489, 869, 1139, 1178
BslFI GGGAC 2 cut(s) 1549, 1715
BsmAI GTCTC 2 cut(s) 1433, 1802
BsmFI GGGAC 2 cut(s) 1549, 1715
BsmI GAATGC 3 cut(s) 731, 785, 1645
BsnI GGCC 4 cut(s) 32, 353, 872, 1350
Bso31I GGTCTC 1 cut(s) 1433
BsoBI CYCGRG 2 cut(s) 904, 1742
Bsp1286I GDGCHC 7 cut(s) 704, 752, 806, 854, 1220, 1380, 1513
Bsp143I GATC 8 cut(s) 20, 62, 110, 152, 284, 1599, 1746, 1886
Bsp1720I GCTNAGC 4 cut(s) 921, 1255, 1644, 1860
BspANI GGCC 4 cut(s) 32, 353, 872, 1350
BspCNI CTCAG 5 cut(s) 646, 1268, 1588, 1636, 1852
BspFNI CGCG 3 cut(s) 29, 119, 665
BspMAI CTGCAG 4 cut(s) 715, 763, 817, 865
BspMI ACCTGC 1 cut(s) 1321
BspQI GCTCTTC 5 cut(s) 709, 757, 811, 859, 889
BspT107I GGYRCC 4 cut(s) 245, 613, 955, 1478
BspTNI GGTCTC 1 cut(s) 1433
BsrBI CCGCTC 2 cut(s) 173, 1789
BsrDI GCAATG 2 cut(s) 1042, 1932
BsrFI RCCGGY 2 cut(s) 353, 1177
BsrI ACTGG 6 cut(s) 1365, 1480, 1482, 1609, 1666, 1703
BssAI RCCGGY 2 cut(s) 353, 1177
BssECI CCNNGG 5 cut(s) 27, 72, 663, 1005, 1122
BssMI GATC 8 cut(s) 20, 62, 110, 152, 284, 1599, 1746, 1886
BssNI GRCGYC 2 cut(s) 614, 956
Bst4CI ACNGT 4 cut(s) 1111, 1567, 1615, 1724
Bst6I CTCTTC 7 cut(s) 110, 607, 709, 757, 811, 859, 889
BstACI GRCGYC 2 cut(s) 614, 956
BstAPI GCANNNNNTGC 1 cut(s) 1247
BstDEI CTNAG 8 cut(s) 633, 921, 1255, 1596, 1644, 1736, 1779, 1860
BstDSI CCRYGG 3 cut(s) 27, 663, 1122
BstENI CCTNNNNNAGG 1 cut(s) 837
BstF5I GGATG 8 cut(s) 215, 419, 452, 791, 935, 1001, 1882, 1910
BstFNI CGCG 3 cut(s) 29, 119, 665
BstH2I RGCGCY 2 cut(s) 617, 959
BstHHI GCGC 4 cut(s) 121, 433, 616, 958
BstKTI GATC 8 cut(s) 23, 65, 113, 155, 287, 1602, 1749, 1889
BstMAI GTCTC 2 cut(s) 1433, 1802
BstMBI GATC 8 cut(s) 20, 62, 110, 152, 284, 1599, 1746, 1886
BstMCI CGRYCG 1 cut(s) 354
BstSCI CCNGG 3 cut(s) 72, 137, 439
BstSFI CTRYAG 5 cut(s) 711, 759, 813, 861, 1563
BstSLI GKGCMC 2 cut(s) 1380, 1513
BstUI CGCG 3 cut(s) 29, 119, 665
BstV2I GAAGAC 2 cut(s) 1080, 1616
BstX2I RGATCY 5 cut(s) 20, 62, 110, 152, 284
BstXI CCANNNNNNTGG 1 cut(s) 745
BstYI RGATCY 5 cut(s) 20, 62, 110, 152, 284
BstZI CGGCCG 1 cut(s) 351
BsuRI GGCC 4 cut(s) 32, 353, 872, 1350
BtgI CCRYGG 3 cut(s) 27, 663, 1122
BtgZI GCGATG 1 cut(s) 1077
BtsCI GGATG 8 cut(s) 215, 419, 452, 791, 935, 1001, 1882, 1910
BtsI GCAGTG 3 cut(s) 1221, 1248, 1455
BtsIMutI CAGTG 6 cut(s) 1221, 1226, 1248, 1372, 1455, 1602
BveI ACCTGC 1 cut(s) 1321
CaiI CAGNNNCTG 4 cut(s) 917, 1247, 1262, 1445
CfoI GCGC 4 cut(s) 121, 433, 616, 958
Cfr10I RCCGGY 2 cut(s) 353, 1177
Cfr42I CCGCGG 2 cut(s) 30, 666
Csp6I GTAC 6 cut(s) 246, 543, 1152, 1479, 1485, 1659
CviAII CATG 1 cut(s) 1846
CviQI GTAC 6 cut(s) 246, 543, 1152, 1479, 1485, 1659
DdeI CTNAG 8 cut(s) 633, 921, 1255, 1596, 1644, 1736, 1779, 1860
DinI GGCGCC 2 cut(s) 615, 957
DpnI GATC 8 cut(s) 22, 64, 112, 154, 286, 1601, 1748, 1888
DpnII GATC 8 cut(s) 20, 62, 110, 152, 284, 1599, 1746, 1886
EaeI YGGCCR 2 cut(s) 351, 870
EagI CGGCCG 1 cut(s) 351
Eam1104I CTCTTC 7 cut(s) 110, 607, 709, 757, 811, 859, 889
EarI CTCTTC 7 cut(s) 110, 607, 709, 757, 811, 859, 889
EciI GGCGGA 1 cut(s) 1054
Ecl136II GAGCTC 3 cut(s) 702, 804, 852
EclXI CGGCCG 1 cut(s) 351
Eco24I GRGCYC 4 cut(s) 704, 752, 806, 854
Eco31I GGTCTC 1 cut(s) 1433
Eco52I CGGCCG 1 cut(s) 351
Eco53kI GAGCTC 3 cut(s) 702, 804, 852
Eco57I CTGAAG 1 cut(s) 1422
Eco88I CYCGRG 2 cut(s) 904, 1742
EcoICRI GAGCTC 3 cut(s) 702, 804, 852
EcoNI CCTNNNNNAGG 1 cut(s) 837
EcoT38I GRGCYC 4 cut(s) 704, 752, 806, 854
EgeI GGCGCC 2 cut(s) 615, 957
EheI GGCGCC 2 cut(s) 615, 957
FaeI CATG 1 cut(s) 1849
FaiI YATR 4 cut(s) 383, 1847, 1868, 1870
FaqI GGGAC 2 cut(s) 1549, 1715
FatI CATG 1 cut(s) 1845
FauI CCCGC 4 cut(s) 130, 244, 790, 1387
FokI GGATG 8 cut(s) 202, 406, 439, 778, 922, 988, 1889, 1897
FriOI GRGCYC 4 cut(s) 704, 752, 806, 854
FspBI CTAG 2 cut(s) 1530, 1796
GlaI GCGC 4 cut(s) 120, 432, 615, 957
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 1 cut(s) 721
HaeII RGCGCY 2 cut(s) 617, 959
HaeIII GGCC 4 cut(s) 32, 353, 872, 1350
HapII CCGG 8 cut(s) 74, 138, 354, 440, 489, 869, 1139, 1178
HhaI GCGC 4 cut(s) 121, 433, 616, 958
Hin1I GRCGYC 2 cut(s) 614, 956
Hin1II CATG 1 cut(s) 1849
Hin6I GCGC 4 cut(s) 119, 431, 614, 956
HinP1I GCGC 4 cut(s) 119, 431, 614, 956
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HindIII AAGCTT 1 cut(s) 691
HinfI GANTC 6 cut(s) 147, 279, 336, 1029, 1192, 1810
HpaII CCGG 8 cut(s) 74, 138, 354, 440, 489, 869, 1139, 1178
HphI GGTGA 3 cut(s) 318, 522, 888
Hpy166II GTNNAC 2 cut(s) 468, 1511
Hpy188III TCNNGA 5 cut(s) 904, 1114, 1652, 1744, 1895
Hpy8I GTNNAC 2 cut(s) 468, 1511
Hpy99I CGWCG 3 cut(s) 376, 661, 1474
HpyAV CCTTC 6 cut(s) 108, 195, 630, 969, 1029, 1602
HpyCH4III ACNGT 4 cut(s) 1111, 1567, 1615, 1724
HpyF3I CTNAG 8 cut(s) 633, 921, 1255, 1596, 1644, 1736, 1779, 1860
Hsp92I GRCGYC 2 cut(s) 614, 956
Hsp92II CATG 1 cut(s) 1849
HspAI GCGC 4 cut(s) 119, 431, 614, 956
KasI GGCGCC 2 cut(s) 613, 955
KpnI GGTACC 2 cut(s) 249, 1482
KroI GCCGGC 1 cut(s) 1177
KroNI GCCGGC 1 cut(s) 1179
KspI CCGCGG 2 cut(s) 30, 666
Kzo9I GATC 8 cut(s) 20, 62, 110, 152, 284, 1599, 1746, 1886
LguI GCTCTTC 5 cut(s) 709, 757, 811, 859, 889
LweI GCATC 5 cut(s) 482, 773, 944, 1912, 1919
MaeI CTAG 2 cut(s) 1530, 1796
MaeIII GTNAC 2 cut(s) 1111, 1189
MalI GATC 8 cut(s) 22, 64, 112, 154, 286, 1601, 1748, 1888
MbiI CCGCTC 2 cut(s) 173, 1789
MboI GATC 8 cut(s) 20, 62, 110, 152, 284, 1599, 1746, 1886
MflI RGATCY 5 cut(s) 20, 62, 110, 152, 284
MhlI GDGCHC 7 cut(s) 704, 752, 806, 854, 1220, 1380, 1513
MluCI AATT 4 cut(s) 13, 1586, 1667, 1803
Mly113I GGCGCC 2 cut(s) 614, 956
MlyI GAGTC 4 cut(s) 156, 288, 1186, 1804
MmeI TCCRAC 5 cut(s) 130, 243, 262, 695, 1523
MroNI GCCGGC 1 cut(s) 1177
MseI TTAA 3 cut(s) 1307, 1551, 1802
MspA1I CMGCKG 6 cut(s) 29, 665, 812, 972, 1058, 1259
MspI CCGG 8 cut(s) 74, 138, 354, 440, 489, 869, 1139, 1178
MspR9I CCNGG 3 cut(s) 74, 139, 441
Mva1269I GAATGC 3 cut(s) 731, 785, 1645
MvnI CGCG 3 cut(s) 29, 119, 665
NaeI GCCGGC 1 cut(s) 1179
NarI GGCGCC 2 cut(s) 614, 956
NciI CCSGG 3 cut(s) 74, 139, 441
NdeII GATC 8 cut(s) 20, 62, 110, 152, 284, 1599, 1746, 1886
NgoMIV GCCGGC 1 cut(s) 1177
NlaIII CATG 1 cut(s) 1849
NmeAIII GCCGAG 2 cut(s) 560, 986
NmuCI GTSAC 2 cut(s) 1111, 1189
PaeR7I CTCGAG 1 cut(s) 1742
PaqCI CACCTGC 1 cut(s) 1321
PciSI GCTCTTC 5 cut(s) 709, 757, 811, 859, 889
PctI GAATGC 3 cut(s) 731, 785, 1645
PdiI GCCGGC 1 cut(s) 1179
PfeI GAWTC 2 cut(s) 336, 1029
PleI GAGTC 4 cut(s) 155, 287, 1186, 1804
PluTI GGCGCC 2 cut(s) 617, 959
PpsI GAGTC 4 cut(s) 155, 287, 1186, 1804
Psp124BI GAGCTC 3 cut(s) 704, 806, 854
PspFI CCCAGC 1 cut(s) 569
PstI CTGCAG 4 cut(s) 715, 763, 817, 865
PstNI CAGNNNCTG 4 cut(s) 917, 1247, 1262, 1445
PsuI RGATCY 5 cut(s) 20, 62, 110, 152, 284
PvuII CAGCTG 1 cut(s) 1259
RsaI GTAC 6 cut(s) 247, 544, 1153, 1480, 1486, 1660
RsaNI GTAC 6 cut(s) 246, 543, 1152, 1479, 1485, 1659
SacI GAGCTC 3 cut(s) 704, 806, 854
SacII CCGCGG 2 cut(s) 30, 666
SapI GCTCTTC 5 cut(s) 709, 757, 811, 859, 889
SaqAI TTAA 3 cut(s) 1307, 1551, 1802
Sau3AI GATC 8 cut(s) 20, 62, 110, 152, 284, 1599, 1746, 1886
ScaI AGTACT 1 cut(s) 1153
SchI GAGTC 4 cut(s) 156, 288, 1186, 1804
ScrFI CCNGG 3 cut(s) 74, 139, 441
SduI GDGCHC 7 cut(s) 704, 752, 806, 854, 1220, 1380, 1513
SfaNI GCATC 5 cut(s) 482, 773, 944, 1912, 1919
SfcI CTRYAG 5 cut(s) 711, 759, 813, 861, 1563
SfoI GGCGCC 2 cut(s) 615, 957
Sfr274I CTCGAG 1 cut(s) 1742
Sfr303I CCGCGG 2 cut(s) 30, 666
SgrBI CCGCGG 2 cut(s) 30, 666
SlaI CTCGAG 1 cut(s) 1742
SmlI CTYRAG 1 cut(s) 1742
SmoI CTYRAG 1 cut(s) 1742
SpeI ACTAGT 1 cut(s) 1529
Sse9I AATT 4 cut(s) 13, 1586, 1667, 1803
SspDI GGCGCC 2 cut(s) 613, 955
SspMI CTAG 2 cut(s) 1530, 1796
SstI GAGCTC 3 cut(s) 704, 806, 854
StyD4I CCNGG 3 cut(s) 72, 137, 439
TaaI ACNGT 4 cut(s) 1111, 1567, 1615, 1724
TaqI TCGA 6 cut(s) 698, 800, 848, 1743, 1749, 1827
TasI AATT 4 cut(s) 13, 1586, 1667, 1803
TatI WGTACW 1 cut(s) 1151
TfiI GAWTC 2 cut(s) 336, 1029
Tru1I TTAA 3 cut(s) 1307, 1551, 1802
Tru9I TTAA 3 cut(s) 1307, 1551, 1802
TscAI CASTG 6 cut(s) 1228, 1233, 1248, 1372, 1462, 1609
TseFI GTSAC 2 cut(s) 1111, 1189
Tsp45I GTSAC 2 cut(s) 1111, 1189
TspDTI ATGAA 6 cut(s) 594, 951, 1080, 1257, 1344, 1917
TspGWI ACGGA 2 cut(s) 360, 645
TspRI CASTG 6 cut(s) 1228, 1233, 1248, 1372, 1462, 1609
VneI GTGCAC 1 cut(s) 1509
XagI CCTNNNNNAGG 1 cut(s) 837
XapI RAATTY 1 cut(s) 1667
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XhoI CTCGAG 1 cut(s) 1742
XspI CTAG 2 cut(s) 1530, 1796
ZrmI AGTACT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.