FvH4_7g10470

Nsp1-like C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013006
Physical Location & Seq
Forward (+)
9884665 .. 9890077
5413 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g10470.t2

Sequence Viewer

Length: 2271 bp
ATGTCGGGTTTCAATTTCGGATCCTCCGCTTCACAATCCACTTCCCCTTTCGGATCCTCCGCTTCTTCTTTTTCATTCGCCACCTCCGGTTCCCTATTCGGATCCTCCGCGCCCGCCACCGCCTCCACCGCCTTCGGAGCCGGATCCTCCCCTTTCGCCTCCGCACCTTCCTCCTCCCCTTTCGGCTTCTCTTCCAACACCGCCTCCTCCCCCGCTTTCGGCACCCCAAACCCTAGCCCCGCCCCGAACCCGTTTCCCTCATTCGGTGTCGGATCCTCCACTGCCTCAACAACCACCGCCACCTCCCCTTTCGGATTCAACCCCTCCACCGCCGCTGCGGGCTCTGCTCCGGCTACGTTCGGCTCAACCCCATCCGCGTCGCTGTTTGGCGCATCCTCATCGGCGCAGGCTTCGGGTTCTACCACATCGCTATTCGGGTCGTCCCCGTTCGGGTCAACTGCGTCCGCGTCCACTTCCGCCGCTACTAGCTCTCCTCTCTTCGGCTCCGCTCCGGCTTCATCTTCACCTTTGTTTGGGACTACTGGCTCTTCCTTCGCTCCCAGCACAGGCTCTGCTCTGTTCGGCTCATCCACATCATCAGCAGCGCCGCCCTCTTCCACCACTCAGAACTTGTTCGGCTCTGCTGCTCCGTCGTCTGCCGCTCCAACCACTTCCTCATTCCCGAGCTTGAGCTTTTCGACCTCTTCTTCGTCCGCAGCCTCCACCACTCCCGCATTCTCGAGCTTTTTGACCTCTTCTTCCTCTGCAGCCTCAACCGCTGCTCCTGCATTCTCCAACTTTTTGAGCTCTTCGTCTTCTGCGGCATCATCAACCGCTCCTGCTCCGGCATTCCCATCCTTTTCGAGCTCCACTCCTGCTTTCGCAGGCTTTCCGAGTGCTTCATCTGCAGCTCCGGCCACTTCTGTGTTCACAAGCTCCTCCAGCTTCTCATCATTCCCGAGTTCAGCTCCTGCCAAGCCGACAGCAGCATCCACACCCAGTACTACTACGGTGCCTTCGTCAAGCGGCGGTTTCGGCCTCTCATTTGGAACCCCTGCATCCTCGGCCTCACAACCTGCGTTCGGATTCACCAACACCAATGCAGCGTCATCAGCAGCGCCGGCTTCCACCTCATTGTCATTTTCAAAGTCTTCATCGCCATTGTTTTCGACTGTCACCACCACAACAACGGCTTCTTCAACTCCGGCGGCAAGTAGCACTACACAAGCTGCTTTCTCTTTGTCAGCATTCAATGCGACTCCTAGTGCACCTCCTGCTCCGAGCACTGCTGCACCTACTTCAACTGCAGTACCTGCTAGCTCAGCTGCTCCTTCATCAGGCACTACTACGAGTTCCTTTACCGGCTTTTCCTTAACACCTGCTTCTTCAGCGGCTACTACTTCTTCTTCTGGCCCTTCAATGTTTTCCGGTGCCTCTGTGCCTGCATCCTCATCGCAAGCACAACCAAGCACTACTGTGCCTGCATTTGGTCTCACTACTTCAGCAACTGCAACAACCACTGCAACTACAACGACAACTGGTACCAGTACCCCTGCAGCTCAGACATCAACAGCACTTGCTGTGGCTTCTACTAGTGGGACAACCTCAAATGTTAATGGCAGCACTACAGTATCTACGACAACACCAAAGTTACCTTCTGAGATCACTGGGAAGACTGTTGAGGAGATCATCAAGGAGTGGAATGCTGAGCTTCAAGAGCGTACTGGAAAGTTCCGAAAGCAGGCTAATGCAATAGCAGACTGGGACAGGAGGATTTTGCAGAATCGTGATGTTCTTCTTAGGCTTGAGATCGAAGTGGCGAATGTGGTGGATACCCAAGCTAAGGTGGAGCGGCAATTAGAGTTAATCGAGACTCATCAGGATGAGGTCGATAAGGCTTTACAGAGCATAGAACAAGAAGCTGAGTGCATATACCAGGATGAGCGTGGATTGCTTCTTAATGATGAAGCTGCATCCACCAGAGATGCAATGTATGAGCAGGCTGAGTATATAGAAAGGGAGCTAGAGCAGGTGACAGAACAAATTAAATCAATCATTGAGACTGTAAATGCCAACCAGGGAGGAGAATTGGACGTGAATGATGGGATGGCTCCACTAGATGTGGTTGTAAAAATCTTAAACAACCAGCTAAGCTCTTTAATGTGGATTGATGAAAAGGCCGAAGAGTTCTCTTCACGAATTCAGAAGCTTGCCAATCAAGGTCCTGCTGCAGATCGTGAATTGATGGGTCCAAGATACTGGATGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0003674 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005642 GO:0005643 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0008092 GO:0008104 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008285 GO:0008289 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010648 GO:0010928 GO:0010930 GO:0010941 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016043 GO:0016070 GO:0017038 GO:0017056 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019894 GO:0019904 GO:0019953 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030159 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032182 GO:0032386 GO:0032501 GO:0032504 GO:0032774 GO:0032879 GO:0032880 GO:0032947 GO:0032991 GO:0033036 GO:0033157 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0042127 GO:0042169 GO:0042175 GO:0042306 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043123 GO:0043130 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044613 GO:0044703 GO:0045184 GO:0045893 GO:0045935 GO:0046483 GO:0046822 GO:0046907 GO:0046966 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051223 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051259 GO:0051291 GO:0051427 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0060255 GO:0060341 GO:0060548 GO:0065003 GO:0065007 GO:0070013 GO:0070201 GO:0070206 GO:0070208 GO:0070727 GO:0071166 GO:0071426 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0098589 GO:0098805 GO:1900180 GO:1901360 GO:1901362 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1903827 GO:1904589 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nsp1_C Nucleoporin_FG

Protein Analysis

757

Amino Acids

75.04

Weight (kDa)

4.56

Isoelectric Point (pI)

60.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nsp1_C PF05064 546 - 646 1.3e-21 Nsp1-like C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45000
fragaria_vesca FvH4_7g10470 FvH4_7g10470 FvH4_7g10470
malus_domestica MD07G1110700.v1.1
prunus_persica Prupe.2G134200_v2.0.a1 Prupe.2G134200_v2.0.a1
pyrus_communis pycom07g09560
rosa_chinensis RchiOBHm_Chr1g0348261
rosa_laevigata RLG00000028672 RLG00000028676
rosa_roxburghii Rroxscaffold_4G00307070
rosa_rugosa Rorug01G0194000
rosa_samantha Rh1AG211000 Rh1BG177500 Rh1CG195400 Rh1DG207000
rosa_wichuraiana Rw1G017820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1387, 2020
Acc36I ACCTGC 4 cut(s) 1084, 1321, 1387, 2020
Acc65I GGTACC 1 cut(s) 1541
AccB1I GGYRCC 4 cut(s) 221, 1012, 1430, 1541
AccBSI CCGCTC 4 cut(s) 509, 662, 836, 1852
AccII CGCG 3 cut(s) 110, 377, 467
AcoI YGGCCR 1 cut(s) 915
AcsI RAATTY 1 cut(s) 2199
AcuI CTGAAG 2 cut(s) 1371, 1485
AfaI GTAC 5 cut(s) 1003, 1311, 1543, 1549, 1723
AgsI TTSAA 8 cut(s) 13, 319, 1146, 1200, 1252, 1302, 1419, 1715
AhlI ACTAGT 1 cut(s) 1592
AjiI CACGTC 1 cut(s) 2095
AjnI CCWGG 2 cut(s) 1935, 2076
AjuI GAANNNNNNNTTGG 2 cut(s) 1639, 1671
AleI CACNNNNGTG 2 cut(s) 923, 1475
Alw21I GWGCWC 4 cut(s) 809, 869, 1270, 1286
Alw26I GTCTC 3 cut(s) 1496, 1865, 2054
Alw44I GTGCAC 1 cut(s) 1266
AlwNI CAGNNNCTG 4 cut(s) 572, 971, 1313, 1508
Ama87I CYCGRG 3 cut(s) 682, 739, 958
AoxI GGCC 5 cut(s) 915, 1036, 1065, 1411, 2178
ApaLI GTGCAC 1 cut(s) 1266
ApoI RAATTY 1 cut(s) 2199
ArsI GACNNNNNNTTYG 2 cut(s) 691, 723
Asp700I GAANNNNTTC 1 cut(s) 632
Asp718I GGTACC 1 cut(s) 1541
AspLEI GCGC 5 cut(s) 112, 392, 406, 607, 1120
AspS9I GGNCC 3 cut(s) 1412, 2222, 2249
AsuHPI GGTGA 4 cut(s) 516, 1081, 1168, 2044
AsuNHI GCTAGC 1 cut(s) 1316
AvaI CYCGRG 3 cut(s) 682, 739, 958
AvaII GGWCC 2 cut(s) 2222, 2249
BaeGI GKGCMC 1 cut(s) 1270
BaeI ACNNNNGTAYC 4 cut(s) 1293, 1326, 1614, 1647
BamHI GGATCC 5 cut(s) 20, 53, 101, 143, 272
BanI GGYRCC 4 cut(s) 221, 1012, 1430, 1541
BanII GRGCYC 3 cut(s) 344, 809, 869
BbsI GAAGAC 3 cut(s) 807, 1143, 1679
Bbv12I GWGCWC 4 cut(s) 809, 869, 1270, 1286
BccI CCATC 5 cut(s) 379, 862, 2096, 2101, 2239
BceAI ACGGC 1 cut(s) 1206
BcgI CGANNNNNNTGC 4 cut(s) 381, 415, 1434, 1468
BciT130I CCWGG 2 cut(s) 1937, 2078
BciVI GTATCC 1 cut(s) 1825
BcoDI GTCTC 3 cut(s) 1496, 1865, 2054
BcuI ACTAGT 1 cut(s) 1592
BfaI CTAG 7 cut(s) 234, 486, 1263, 1317, 1593, 2024, 2117
BfmI CTRYAG 6 cut(s) 765, 906, 1305, 1554, 1626, 2229
BfoI RGCGCY 2 cut(s) 608, 1121
BfuAI ACCTGC 4 cut(s) 1084, 1321, 1387, 2020
BfuI GTATCC 1 cut(s) 1825
BlpI GCTNAGC 3 cut(s) 1321, 1707, 2150
BmcAI AGTACT 1 cut(s) 1003
Bme1390I CCNGG 2 cut(s) 1937, 2078
Bme18I GGWCC 2 cut(s) 2222, 2249
BmeT110I CYCGRG 3 cut(s) 682, 739, 958
BmgBI CACGTC 1 cut(s) 2095
BmgT120I GGNCC 3 cut(s) 1412, 2222, 2249
BmrFI CCNGG 2 cut(s) 1937, 2078
BmrI ACTGGG 3 cut(s) 993, 1677, 1771
BmsI GCATC 7 cut(s) 401, 833, 998, 1067, 1454, 1975, 1982
BmtI GCTAGC 1 cut(s) 1320
BmuI ACTGGG 3 cut(s) 993, 1677, 1771
BpiI GAAGAC 3 cut(s) 807, 1143, 1679
BplI GAGNNNNNCTC 4 cut(s) 856, 888, 952, 984
BpmI CTGGAG 1 cut(s) 925
Bpu10I CCTNAGC 1 cut(s) 1842
Bpu1102I GCTNAGC 3 cut(s) 1321, 1707, 2150
BpuEI CTTGAG 2 cut(s) 709, 1826
BsaI GGTCTC 1 cut(s) 1496
BsaJI CCNNGG 2 cut(s) 1062, 2077
BsaWI WCCGGW 2 cut(s) 86, 1427
BsaXI ACNNNNNCTCC 6 cut(s) 188, 218, 475, 505, 766, 796
Bse118I RCCGGY 2 cut(s) 1120, 1361
Bse1I ACTGG 8 cut(s) 547, 999, 1543, 1545, 1672, 1729, 1766, 2264
Bse3DI GCAATG 1 cut(s) 1995
BseBI CCWGG 2 cut(s) 1937, 2078
BseDI CCNNGG 2 cut(s) 1062, 2077
BseMI GCAATG 1 cut(s) 1995
BseMII CTCAG 7 cut(s) 638, 1335, 1574, 1650, 1698, 1914, 1995
BseNI ACTGG 8 cut(s) 547, 999, 1543, 1545, 1672, 1729, 1766, 2264
BseRI GAGGAG 6 cut(s) 163, 196, 483, 928, 1697, 2097
BseSI GKGCMC 1 cut(s) 1270
BseYI CCCAGC 1 cut(s) 560
BsgI GTGCAG 1 cut(s) 1275
Bsh1236I CGCG 3 cut(s) 110, 377, 467
BshFI GGCC 5 cut(s) 917, 1038, 1067, 1413, 2180
BshNI GGYRCC 4 cut(s) 221, 1012, 1430, 1541
BsiHKAI GWGCWC 4 cut(s) 809, 869, 1270, 1286
BsiHKCI CYCGRG 3 cut(s) 682, 739, 958
BslFI GGGAC 4 cut(s) 427, 550, 1612, 1778
BsmAI GTCTC 3 cut(s) 1496, 1865, 2054
BsmFI GGGAC 4 cut(s) 427, 550, 1612, 1778
BsmI GAATGC 5 cut(s) 734, 788, 848, 1247, 1708
BsnI GGCC 5 cut(s) 917, 1038, 1067, 1413, 2180
Bso31I GGTCTC 1 cut(s) 1496
BsoBI CYCGRG 3 cut(s) 682, 739, 958
Bsp1286I GDGCHC 5 cut(s) 344, 809, 869, 1270, 1286
Bsp143I GATC 9 cut(s) 20, 53, 101, 143, 272, 1662, 1686, 1809, 2233
Bsp1720I GCTNAGC 3 cut(s) 1321, 1707, 2150
BspANI GGCC 5 cut(s) 917, 1038, 1067, 1413, 2180
BspCNI CTCAG 7 cut(s) 637, 1334, 1573, 1651, 1699, 1915, 1996
BspFNI CGCG 3 cut(s) 110, 377, 467
BspHI TCATGA 1 cut(s) 2267
BspMAI CTGCAG 5 cut(s) 769, 910, 1309, 1558, 2233
BspMI ACCTGC 4 cut(s) 1084, 1321, 1387, 2020
BspOI GCTAGC 1 cut(s) 1320
BspQI GCTCTTC 2 cut(s) 553, 814
BspT107I GGYRCC 4 cut(s) 221, 1012, 1430, 1541
BspTNI GGTCTC 1 cut(s) 1496
BsrBI CCGCTC 4 cut(s) 509, 662, 836, 1852
BsrDI GCAATG 1 cut(s) 1995
BsrFI RCCGGY 2 cut(s) 1120, 1361
BsrI ACTGG 8 cut(s) 547, 999, 1543, 1545, 1672, 1729, 1766, 2264
BssAI RCCGGY 2 cut(s) 1120, 1361
BssECI CCNNGG 2 cut(s) 1062, 2077
BssMI GATC 9 cut(s) 20, 53, 101, 143, 272, 1662, 1686, 1809, 2233
Bst2UI CCWGG 2 cut(s) 1937, 2078
Bst4CI ACNGT 6 cut(s) 1012, 1174, 1477, 1630, 1678, 2065
Bst6I CTCTTC 9 cut(s) 196, 503, 553, 619, 709, 760, 814, 2178, 2197
BstAPI GCANNNNNTGC 3 cut(s) 1253, 1274, 1313
BstENI CCTNNNNNAGG 1 cut(s) 1335
BstFNI CGCG 3 cut(s) 110, 377, 467
BstH2I RGCGCY 2 cut(s) 608, 1121
BstHHI GCGC 5 cut(s) 112, 392, 406, 607, 1120
BstKTI GATC 9 cut(s) 23, 56, 104, 146, 275, 1665, 1689, 1812, 2236
BstMAI GTCTC 3 cut(s) 1496, 1865, 2054
BstMBI GATC 9 cut(s) 20, 53, 101, 143, 272, 1662, 1686, 1809, 2233
BstNI CCWGG 2 cut(s) 1937, 2078
BstSCI CCNGG 2 cut(s) 1935, 2076
BstSFI CTRYAG 6 cut(s) 765, 906, 1305, 1554, 1626, 2229
BstSLI GKGCMC 1 cut(s) 1270
BstUI CGCG 3 cut(s) 110, 377, 467
BstV2I GAAGAC 3 cut(s) 807, 1143, 1679
BstX2I RGATCY 5 cut(s) 20, 53, 101, 143, 272
BstXI CCANNNNNNTGG 1 cut(s) 2259
BstYI RGATCY 5 cut(s) 20, 53, 101, 143, 272
BsuI GTATCC 1 cut(s) 1825
BsuRI GGCC 5 cut(s) 917, 1038, 1067, 1413, 2180
BtgZI GCGATG 3 cut(s) 411, 1140, 1437
BtrI CACGTC 1 cut(s) 2095
BtsI GCAGTG 3 cut(s) 279, 1284, 1518
BtsIMutI CAGTG 4 cut(s) 279, 1284, 1518, 1665
BveI ACCTGC 4 cut(s) 1084, 1321, 1387, 2020
CaiI CAGNNNCTG 4 cut(s) 572, 971, 1313, 1508
CciI TCATGA 1 cut(s) 2267
CfoI GCGC 5 cut(s) 112, 392, 406, 607, 1120
Cfr10I RCCGGY 2 cut(s) 1120, 1361
Cfr13I GGNCC 3 cut(s) 1412, 2222, 2249
CseI GACGC 4 cut(s) 366, 450, 456, 1095
Csp6I GTAC 5 cut(s) 1002, 1310, 1542, 1548, 1722
CviAII CATG 1 cut(s) 2268
CviQI GTAC 5 cut(s) 1002, 1310, 1542, 1548, 1722
DpnI GATC 9 cut(s) 22, 55, 103, 145, 274, 1664, 1688, 1811, 2235
DpnII GATC 9 cut(s) 20, 53, 101, 143, 272, 1662, 1686, 1809, 2233
EaeI YGGCCR 1 cut(s) 915
Eam1104I CTCTTC 9 cut(s) 196, 503, 553, 619, 709, 760, 814, 2178, 2197
EarI CTCTTC 9 cut(s) 196, 503, 553, 619, 709, 760, 814, 2178, 2197
EciI GGCGGA 1 cut(s) 466
Ecl136II GAGCTC 2 cut(s) 807, 867
Eco24I GRGCYC 3 cut(s) 344, 809, 869
Eco31I GGTCTC 1 cut(s) 1496
Eco47I GGWCC 2 cut(s) 2222, 2249
Eco53kI GAGCTC 2 cut(s) 807, 867
Eco57I CTGAAG 2 cut(s) 1371, 1485
Eco88I CYCGRG 3 cut(s) 682, 739, 958
EcoICRI GAGCTC 2 cut(s) 807, 867
EcoNI CCTNNNNNAGG 1 cut(s) 1335
EcoO109I RGGNCCY 1 cut(s) 2222
EcoRI GAATTC 1 cut(s) 2199
EcoRII CCWGG 2 cut(s) 1935, 2076
EcoT38I GRGCYC 3 cut(s) 344, 809, 869
FaeI CATG 1 cut(s) 2271
FaiI YATR 7 cut(s) 1910, 1931, 1933, 1995, 2010, 2012, 2269
FaqI GGGAC 4 cut(s) 427, 550, 1612, 1778
FatI CATG 1 cut(s) 2267
FauI CCCGC 5 cut(s) 121, 220, 247, 331, 739
FriOI GRGCYC 3 cut(s) 344, 809, 869
FspBI CTAG 7 cut(s) 234, 486, 1263, 1317, 1593, 2024, 2117
GlaI GCGC 5 cut(s) 111, 391, 405, 606, 1119
GsaI CCCAGC 1 cut(s) 564
GsuI CTGGAG 1 cut(s) 925
HaeII RGCGCY 2 cut(s) 608, 1121
HaeIII GGCC 5 cut(s) 917, 1038, 1067, 1413, 2180
HgaI GACGC 4 cut(s) 366, 450, 456, 1095
HhaI GCGC 5 cut(s) 112, 392, 406, 607, 1120
Hin1II CATG 1 cut(s) 2271
Hin6I GCGC 5 cut(s) 110, 390, 404, 605, 1118
HinP1I GCGC 5 cut(s) 110, 390, 404, 605, 1118
HincII GTYRAC 1 cut(s) 456
HindII GTYRAC 1 cut(s) 456
HindIII AAGCTT 1 cut(s) 2207
HinfI GANTC 5 cut(s) 315, 1086, 1258, 1783, 1873
HphI GGTGA 4 cut(s) 516, 1081, 1168, 2044
Hpy166II GTNNAC 4 cut(s) 456, 471, 930, 1268
Hpy8I GTNNAC 4 cut(s) 456, 471, 930, 1268
Hpy99I CGWCG 2 cut(s) 382, 655
HpyAV CCTTC 7 cut(s) 142, 177, 562, 1026, 1341, 1425, 1665
HpyCH4III ACNGT 6 cut(s) 1012, 1174, 1477, 1630, 1678, 2065
HpyCH4IV ACGT 2 cut(s) 356, 2094
HpySE526I ACGT 2 cut(s) 356, 2094
Hsp92II CATG 1 cut(s) 2271
HspAI GCGC 5 cut(s) 110, 390, 404, 605, 1118
KpnI GGTACC 1 cut(s) 1545
KroI GCCGGC 1 cut(s) 1120
KroNI GCCGGC 1 cut(s) 1122
Kzo9I GATC 9 cut(s) 20, 53, 101, 143, 272, 1662, 1686, 1809, 2233
LguI GCTCTTC 2 cut(s) 553, 814
LweI GCATC 7 cut(s) 401, 833, 998, 1067, 1454, 1975, 1982
MaeI CTAG 7 cut(s) 234, 486, 1263, 1317, 1593, 2024, 2117
MaeII ACGT 2 cut(s) 356, 2094
MaeIII GTNAC 3 cut(s) 1174, 1650, 2032
MalI GATC 9 cut(s) 22, 55, 103, 145, 274, 1664, 1688, 1811, 2235
MbiI CCGCTC 4 cut(s) 509, 662, 836, 1852
MboI GATC 9 cut(s) 20, 53, 101, 143, 272, 1662, 1686, 1809, 2233
MflI RGATCY 5 cut(s) 20, 53, 101, 143, 272
MhlI GDGCHC 5 cut(s) 344, 809, 869, 1270, 1286
MluCI AATT 6 cut(s) 13, 1856, 2043, 2087, 2199, 2240
MlyI GAGTC 2 cut(s) 1252, 1867
MmeI TCCRAC 4 cut(s) 219, 250, 689, 819
MroNI GCCGGC 1 cut(s) 1120
MroXI GAANNNNTTC 1 cut(s) 632
MseI TTAA 7 cut(s) 1373, 1614, 1865, 1959, 2046, 2138, 2159
MslI CAYNNNNRTG 3 cut(s) 923, 1475, 1881
MspA1I CMGCKG 4 cut(s) 335, 779, 1325, 1391
MspR9I CCNGG 2 cut(s) 1937, 2078
Mva1269I GAATGC 5 cut(s) 734, 788, 848, 1247, 1708
MvaI CCWGG 2 cut(s) 1937, 2078
MvnI CGCG 3 cut(s) 110, 377, 467
NaeI GCCGGC 1 cut(s) 1122
NdeII GATC 9 cut(s) 20, 53, 101, 143, 272, 1662, 1686, 1809, 2233
NgoMIV GCCGGC 1 cut(s) 1120
NheI GCTAGC 1 cut(s) 1316
NlaIII CATG 1 cut(s) 2271
NmeAIII GCCGAG 1 cut(s) 1043
NmuCI GTSAC 2 cut(s) 1174, 2032
OliI CACNNNNGTG 2 cut(s) 923, 1475
PaeR7I CTCGAG 1 cut(s) 739
PagI TCATGA 1 cut(s) 2267
PaqCI CACCTGC 2 cut(s) 1387, 2020
PciSI GCTCTTC 2 cut(s) 553, 814
PcsI WCGNNNNNNNCGW 1 cut(s) 1016
PctI GAATGC 5 cut(s) 734, 788, 848, 1247, 1708
PdiI GCCGGC 1 cut(s) 1122
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 3 cut(s) 315, 1086, 1783
PleI GAGTC 2 cut(s) 1252, 1867
PpsI GAGTC 2 cut(s) 1252, 1867
PpuMI RGGWCCY 1 cut(s) 2222
Psp124BI GAGCTC 2 cut(s) 809, 869
Psp5II RGGWCCY 1 cut(s) 2222
Psp6I CCWGG 2 cut(s) 1935, 2076
PspFI CCCAGC 1 cut(s) 560
PspGI CCWGG 2 cut(s) 1935, 2076
PspPI GGNCC 3 cut(s) 1412, 2222, 2249
PspPPI RGGWCCY 1 cut(s) 2222
PstI CTGCAG 5 cut(s) 769, 910, 1309, 1558, 2233
PstNI CAGNNNCTG 4 cut(s) 572, 971, 1313, 1508
PsuI RGATCY 5 cut(s) 20, 53, 101, 143, 272
PvuII CAGCTG 1 cut(s) 1325
RsaI GTAC 5 cut(s) 1003, 1311, 1543, 1549, 1723
RsaNI GTAC 5 cut(s) 1002, 1310, 1542, 1548, 1722
RseI CAYNNNNRTG 3 cut(s) 923, 1475, 1881
SacI GAGCTC 2 cut(s) 809, 869
SapI GCTCTTC 2 cut(s) 553, 814
SaqAI TTAA 7 cut(s) 1373, 1614, 1865, 1959, 2046, 2138, 2159
Sau3AI GATC 9 cut(s) 20, 53, 101, 143, 272, 1662, 1686, 1809, 2233
Sau96I GGNCC 3 cut(s) 1412, 2222, 2249
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 2 cut(s) 1252, 1867
ScrFI CCNGG 2 cut(s) 1937, 2078
SduI GDGCHC 5 cut(s) 344, 809, 869, 1270, 1286
SfaNI GCATC 7 cut(s) 401, 833, 998, 1067, 1454, 1975, 1982
SfcI CTRYAG 6 cut(s) 765, 906, 1305, 1554, 1626, 2229
Sfr274I CTCGAG 1 cut(s) 739
SinI GGWCC 2 cut(s) 2222, 2249
SlaI CTCGAG 1 cut(s) 739
SmiMI CAYNNNNRTG 3 cut(s) 923, 1475, 1881
SmlI CTYRAG 3 cut(s) 688, 739, 1805
SmoI CTYRAG 3 cut(s) 688, 739, 1805
SpeI ACTAGT 1 cut(s) 1592
Sse9I AATT 6 cut(s) 13, 1856, 2043, 2087, 2199, 2240
SspMI CTAG 7 cut(s) 234, 486, 1263, 1317, 1593, 2024, 2117
SstI GAGCTC 2 cut(s) 809, 869
StyD4I CCNGG 2 cut(s) 1935, 2076
TaaI ACNGT 6 cut(s) 1012, 1174, 1477, 1630, 1678, 2065
TaiI ACGT 2 cut(s) 359, 2097
TaqI TCGA 7 cut(s) 698, 740, 863, 1169, 1812, 1869, 1890
TasI AATT 6 cut(s) 13, 1856, 2043, 2087, 2199, 2240
TatI WGTACW 1 cut(s) 1001
TauI GCSGC 9 cut(s) 335, 482, 610, 662, 824, 1029, 1211, 1394, 1855
TfiI GAWTC 3 cut(s) 315, 1086, 1783
Tru1I TTAA 7 cut(s) 1373, 1614, 1865, 1959, 2046, 2138, 2159
Tru9I TTAA 7 cut(s) 1373, 1614, 1865, 1959, 2046, 2138, 2159
TscAI CASTG 4 cut(s) 286, 1291, 1525, 1672
TseFI GTSAC 2 cut(s) 1174, 2032
Tsp45I GTSAC 2 cut(s) 1174, 2032
TspDTI ATGAA 7 cut(s) 63, 507, 891, 1143, 1323, 1980, 2187
TspGWI ACGGA 1 cut(s) 639
TspRI CASTG 4 cut(s) 286, 1291, 1525, 1672
VneI GTGCAC 1 cut(s) 1266
VpaK11BI GGWCC 2 cut(s) 2222, 2249
XagI CCTNNNNNAGG 1 cut(s) 1335
XapI RAATTY 1 cut(s) 2199
XcmI CCANNNNNNNNNTGG 1 cut(s) 1943
XhoI CTCGAG 1 cut(s) 739
XmnI GAANNNNTTC 1 cut(s) 632
XspI CTAG 7 cut(s) 234, 486, 1263, 1317, 1593, 2024, 2117
ZrmI AGTACT 1 cut(s) 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.