RLG00000028672

Nsp1-like C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013006
Physical Location & Seq
Reverse (-)
25940067 .. 25945621
5555 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028672

Sequence Viewer

Length: 2226 bp
ATGTCGGGTTTCAATTTCGGATCCTCCTCGGCCTCCGCTTCACAATCCACTTCCCCTTTCGGATCCTCCACCCCTACTTTTTCATTCGCCTCCTCCCCGTCCCTATTCGCATCCTCCGGGCCCGCCACCGCCTCCACCGGGTTCGGACTCGGATCCTCCTCTTTCGGCTCCGCTCCCTCAAACCCTTCCACCTCCTCCTCTCCTTTCAGCTTCTTATCCAACACCGCCTCCTCCCCCGCTTTCGGTACCCCAAACCCTAGCCCCTCCCCGAACCCAAACCCGAACCCGTTTCCCTCATTCGGAGTCGGATCCTCCACCACCACCACCACAGCAGCTTCTACACCTTCATTCGGATTCACTCCCTCCACCGCCGGTTCCGGCTCCTCTCCGTCGCAGCCTACGTTCGGCTCAACCCCTTCTCAGTCCCTTTTTGGCTCATCCTCATCGGCGCAGGCTCCGGGTTCTACCACATCCTTATTCGGGTCAACTGCATCTGCCTCCACTTCCGGCGCTTCTAGCTCTCCTCTATTCAGCTCGGCTTCACCTTTGTTTGGGACTACTACTTCTTCTTTCGCTCCCAGCACAGGCTCGGCTATCTTTGGCTCGTCCTCTTCATCGGCGGCGCCGCCTTCCTCCACCACTCAGAACTTGTTTGGCTCTGCTCCATCGTCCGCGGCTCCAACCACTCCCTCATTCCCAAGCTTTCTGAGCTCTTCCTCTGCAGCCTCAACCGCTCCTGCATTCCCCAGCTTTTTGAGCGCTTCTTCTGCAGCCTCAACCACTCCCACTCCCGCATTCCCATCCTTTTCGAGCTCTTCCGCTGCAGCCTCAACCACTTCTCCTTTCGCAGGCTTTTCGAGCTCTTCTTCTGCAGCTCCGGTCGCTTCCTCGTTCGCAAGCTCTTCGGGATTCCCATCATTCCCGAGTTCAGCTCCTGCTAAGCCCACAGCAGCATCCACACCTGCTACTACTACTGTGCCTTCATCAGGCAGCGGTTTCAGCCTCTCATTTGGAGCACCGACATCCTCGGCTTCTCAACCTTCTTTTGGATTCAGCAATGCCGCCTCATCAGCCGCTGTGGCTTCCACCGCATTGTCATTTTCAAAGTCTTCATCGCCATTGTTTTCAACCGTCACGACCACCACGGCTTCTTCAACTCCGGCTGCAAGTAGTACTACACAAGCTCCTTTCTCTTTGCCAGCTTTCAATGTGACTCCTACTGCTCCTCCTGCTGCGAGCACCACTGCACTGACTTCAACGGCAGTGCCTGCCAGCTCAGCTGCTTCTTCATCAAGCACTACTACGAGTTCCTTTACAGGTTTCTCCATAACACCTGCTTCTTCAGCGGCTACTGCTTCTTCATCTGGCCTTTCATTGTTTTCCAGTGCCTCTGTGCCCGCCTCGTCATCACAAGCACAACCAAGCACCACTCTGCCCGCATTTGGTCTCACTACTTCAGCAACTGCAACTACCACTGCAACTACAACGACAACTGGTACCAGTACCCCTGCAGCTCTGACATCAAGTGCACTTGCTGTGGCTTCTACTAGTGGGACAACCTCAAATGTCAATGGCAGCACTACAGTATCTACGACAACACCAAAATTACCTTCTGAGATCACTGGGAAGACTGTTGAGGAGATTATCAAGGAGTGGAATGCTGAGCTTCAAGAGCGTACTGGAAAATTCCGAAAGCAGGCTAATGCAATAGCAGACTGGGACAGGAGGATTTTGCAGAACCGTGATGTTCTTCTTAGGCTCGAGATCGAAGTGGCAAATGTGGTAGATACCCAAGCTAAGGTGGAGCGGCAACTAGAGTTAATTGAGACTCATCAGCAAGAGGTCGATAAGGCTTTACAAAGCATGGAACAAGAAGCTGAGCGTATATACAAGGATGAGCGTGGATCACTTCTTGATGATGAAGCTGCATCCACAAGAGATGCAATGTATGAGCAGGCTGAGTTTATAGAGAGGGAGCTAGAGCAGGTGACAGAGCAAATCAAATTAATTATTGAGGCTGTAAATGCCAACCAGGGAGGAGAATTGGACGTAAATGATGGGATGGCTCCACTGGATGTGGTTGTACGAATCTTAAACAACCAACTAAGCTCTTTAATGTGGATTGATGAAAAGGCCGAAGAGTTCTCTTCGCGAATTCAGAAGCTTGCCAATCAAGGTCCTGCTGCAGATCGTGAATTGATGGGTCCAAGATACTGGATGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0003674 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005642 GO:0005643 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0008092 GO:0008104 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008285 GO:0008289 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010648 GO:0010928 GO:0010930 GO:0010941 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016043 GO:0016070 GO:0017038 GO:0017056 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019894 GO:0019904 GO:0019953 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030159 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032182 GO:0032386 GO:0032501 GO:0032504 GO:0032774 GO:0032879 GO:0032880 GO:0032947 GO:0032991 GO:0033036 GO:0033157 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0042127 GO:0042169 GO:0042175 GO:0042306 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043123 GO:0043130 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044613 GO:0044703 GO:0045184 GO:0045893 GO:0045935 GO:0046483 GO:0046822 GO:0046907 GO:0046966 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051223 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051259 GO:0051291 GO:0051427 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0060255 GO:0060341 GO:0060548 GO:0065003 GO:0065007 GO:0070013 GO:0070201 GO:0070206 GO:0070208 GO:0070727 GO:0071166 GO:0071426 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0098589 GO:0098805 GO:1900180 GO:1901360 GO:1901362 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1903827 GO:1904589 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nsp1_C Nucleoporin_FG

Protein Analysis

742

Amino Acids

74.11

Weight (kDa)

4.65

Isoelectric Point (pI)

61.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nsp1_C PF05064 531 - 634 6.6e-22 Nsp1-like C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45000
fragaria_vesca FvH4_7g10470 FvH4_7g10470 FvH4_7g10470
malus_domestica MD07G1110700.v1.1
prunus_persica Prupe.2G134200_v2.0.a1 Prupe.2G134200_v2.0.a1
pyrus_communis pycom07g09560
rosa_chinensis RchiOBHm_Chr1g0348261
rosa_laevigata RLG00000028672 RLG00000028676
rosa_roxburghii Rroxscaffold_4G00307070
rosa_rugosa Rorug01G0194000
rosa_samantha Rh1AG211000 Rh1BG177500 Rh1CG195400 Rh1DG207000
rosa_wichuraiana Rw1G017820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 970, 1342, 1975
Acc36I ACCTGC 3 cut(s) 970, 1342, 1975
Acc65I GGTACC 2 cut(s) 245, 1496
AccB1I GGYRCC 3 cut(s) 245, 622, 1496
AccBSI CCGCTC 3 cut(s) 173, 734, 1807
AccII CGCG 2 cut(s) 674, 2152
AclWI GGATC 9 cut(s) 15, 28, 57, 70, 147, 160, 303, 316, 1912
AcsI RAATTY 2 cut(s) 1685, 2154
AcuI CTGAAG 2 cut(s) 1326, 1440
AcyI GRCGYC 1 cut(s) 623
AfaI GTAC 6 cut(s) 247, 1174, 1498, 1504, 1678, 2085
AfeI AGCGCT 1 cut(s) 760
AgsI TTSAA 7 cut(s) 13, 1104, 1128, 1155, 1207, 1257, 1670
AhlI ACTAGT 1 cut(s) 1547
AjnI CCWGG 1 cut(s) 2031
AjuI GAANNNNNNNTTGG 2 cut(s) 1594, 1626
Alw21I GWGCWC 6 cut(s) 713, 815, 863, 1018, 1241, 1531
Alw26I GTCTC 2 cut(s) 1451, 1820
Alw44I GTGCAC 1 cut(s) 1527
AlwI GGATC 9 cut(s) 15, 28, 57, 70, 147, 160, 303, 316, 1912
AlwNI CAGNNNCTG 4 cut(s) 935, 1076, 1268, 1463
Ama87I CYCGRG 2 cut(s) 922, 1760
Aor51HI AGCGCT 1 cut(s) 760
AoxI GGCC 4 cut(s) 30, 119, 1366, 2133
ApaI GGGCCC 1 cut(s) 123
ApaLI GTGCAC 1 cut(s) 1527
ApoI RAATTY 2 cut(s) 1685, 2154
AseI ATTAAT 1 cut(s) 2006
Asp718I GGTACC 2 cut(s) 245, 1496
AspLEI GCGC 4 cut(s) 451, 512, 625, 761
AspS9I GGNCC 4 cut(s) 119, 120, 2177, 2204
AsuC2I CCSGG 3 cut(s) 118, 139, 459
AsuHPI GGTGA 2 cut(s) 534, 1999
AvaI CYCGRG 2 cut(s) 922, 1760
AvaII GGWCC 2 cut(s) 2177, 2204
BaeGI GKGCMC 3 cut(s) 123, 1398, 1531
BaeI ACNNNNGTAYC 2 cut(s) 1569, 1602
BamHI GGATCC 4 cut(s) 20, 62, 152, 308
BanI GGYRCC 3 cut(s) 245, 622, 1496
BanII GRGCYC 4 cut(s) 123, 713, 815, 863
BbsI GAAGAC 2 cut(s) 1101, 1634
Bbv12I GWGCWC 6 cut(s) 713, 815, 863, 1018, 1241, 1531
BccI CCATC 6 cut(s) 673, 808, 922, 2051, 2056, 2194
BceAI ACGGC 2 cut(s) 1161, 1275
BcgI CGANNNNNNTGC 4 cut(s) 837, 871, 885, 919
BciT130I CCWGG 1 cut(s) 2033
BcnI CCSGG 3 cut(s) 118, 139, 459
BcoDI GTCTC 2 cut(s) 1451, 1820
BcuI ACTAGT 1 cut(s) 1547
BfaI CTAG 5 cut(s) 258, 516, 1548, 1814, 1979
BfmI CTRYAG 7 cut(s) 720, 768, 822, 870, 1509, 1581, 2184
BfoI RGCGCY 3 cut(s) 513, 626, 762
BfuAI ACCTGC 3 cut(s) 970, 1342, 1975
BglI GCCNNNNNGGC 1 cut(s) 1079
BlpI GCTNAGC 4 cut(s) 939, 1276, 1662, 1878
BmcAI AGTACT 1 cut(s) 1174
Bme1390I CCNGG 4 cut(s) 118, 139, 459, 2033
Bme18I GGWCC 2 cut(s) 2177, 2204
BmeT110I CYCGRG 2 cut(s) 922, 1760
BmgT120I GGNCC 4 cut(s) 119, 120, 2177, 2204
BmrFI CCNGG 4 cut(s) 118, 139, 459, 2033
BmrI ACTGGG 2 cut(s) 1632, 1726
BmsI GCATC 5 cut(s) 119, 500, 962, 1930, 1937
BmuI ACTGGG 2 cut(s) 1632, 1726
BpiI GAAGAC 2 cut(s) 1101, 1634
BplI GAGNNNNNCTC 2 cut(s) 916, 948
Bpu10I CCTNAGC 1 cut(s) 1797
Bpu1102I GCTNAGC 4 cut(s) 939, 1276, 1662, 1878
BpuMI CCSGG 3 cut(s) 118, 139, 459
BsaBI GATNNNNATC 1 cut(s) 913
BsaHI GRCGYC 1 cut(s) 623
BsaI GGTCTC 1 cut(s) 1451
BsaJI CCNNGG 5 cut(s) 27, 672, 1026, 1143, 2032
BsaWI WCCGGW 1 cut(s) 877
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 8 cut(s) 1383, 1498, 1500, 1627, 1684, 1721, 2076, 2219
Bse3DI GCAATG 2 cut(s) 1063, 1950
Bse8I GATNNNNATC 1 cut(s) 913
BseBI CCWGG 1 cut(s) 2033
BseDI CCNNGG 5 cut(s) 27, 672, 1026, 1143, 2032
BseJI GATNNNNATC 1 cut(s) 913
BseMI GCAATG 2 cut(s) 1063, 1950
BseMII CTCAG 8 cut(s) 434, 656, 698, 1290, 1605, 1653, 1869, 1950
BseNI ACTGG 8 cut(s) 1383, 1498, 1500, 1627, 1684, 1721, 2076, 2219
BseSI GKGCMC 3 cut(s) 123, 1398, 1531
BseYI CCCAGC 2 cut(s) 578, 746
BsgI GTGCAG 1 cut(s) 1230
Bsh1236I CGCG 2 cut(s) 674, 2152
Bsh1285I CGRYCG 1 cut(s) 882
BshFI GGCC 4 cut(s) 32, 121, 1368, 2135
BshNI GGYRCC 3 cut(s) 245, 622, 1496
BsiEI CGRYCG 1 cut(s) 882
BsiHKAI GWGCWC 6 cut(s) 713, 815, 863, 1018, 1241, 1531
BsiHKCI CYCGRG 2 cut(s) 922, 1760
BsiSI CCGG 8 cut(s) 117, 138, 372, 378, 458, 507, 878, 1160
BslFI GGGAC 5 cut(s) 85, 409, 568, 1567, 1733
BsmAI GTCTC 2 cut(s) 1451, 1820
BsmFI GGGAC 5 cut(s) 85, 409, 568, 1567, 1733
BsmI GAATGC 3 cut(s) 740, 794, 1663
BsnI GGCC 4 cut(s) 32, 121, 1368, 2135
Bso31I GGTCTC 1 cut(s) 1451
BsoBI CYCGRG 2 cut(s) 922, 1760
Bsp120I GGGCCC 1 cut(s) 119
Bsp1286I GDGCHC 8 cut(s) 123, 713, 815, 863, 1018, 1241, 1398, 1531
Bsp143I GATC 8 cut(s) 20, 62, 152, 308, 1617, 1764, 1904, 2188
Bsp1720I GCTNAGC 4 cut(s) 939, 1276, 1662, 1878
Bsp68I TCGCGA 1 cut(s) 2152
BspANI GGCC 4 cut(s) 32, 121, 1368, 2135
BspCNI CTCAG 8 cut(s) 433, 655, 699, 1289, 1606, 1654, 1870, 1951
BspFNI CGCG 2 cut(s) 674, 2152
BspHI TCATGA 1 cut(s) 2222
BspMAI CTGCAG 6 cut(s) 724, 772, 826, 874, 1513, 2188
BspMI ACCTGC 3 cut(s) 970, 1342, 1975
BspPI GGATC 9 cut(s) 15, 28, 57, 70, 147, 160, 303, 316, 1912
BspQI GCTCTTC 4 cut(s) 718, 820, 868, 907
BspT107I GGYRCC 3 cut(s) 245, 622, 1496
BspTNI GGTCTC 1 cut(s) 1451
BsrBI CCGCTC 3 cut(s) 173, 734, 1807
BsrDI GCAATG 2 cut(s) 1063, 1950
BsrFI RCCGGY 1 cut(s) 371
BsrI ACTGG 8 cut(s) 1383, 1498, 1500, 1627, 1684, 1721, 2076, 2219
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 5 cut(s) 27, 672, 1026, 1143, 2032
BssMI GATC 8 cut(s) 20, 62, 152, 308, 1617, 1764, 1904, 2188
BssNI GRCGYC 1 cut(s) 623
Bst2UI CCWGG 1 cut(s) 2033
Bst4CI ACNGT 5 cut(s) 976, 1132, 1585, 1633, 1742
Bst6I CTCTTC 7 cut(s) 616, 718, 820, 868, 907, 2133, 2152
BstACI GRCGYC 1 cut(s) 623
BstAPI GCANNNNNTGC 1 cut(s) 1268
BstDSI CCRYGG 2 cut(s) 672, 1143
BstENI CCTNNNNNAGG 2 cut(s) 846, 984
BstFNI CGCG 2 cut(s) 674, 2152
BstH2I RGCGCY 3 cut(s) 513, 626, 762
BstHHI GCGC 4 cut(s) 451, 512, 625, 761
BstKTI GATC 8 cut(s) 23, 65, 155, 311, 1620, 1767, 1907, 2191
BstMAI GTCTC 2 cut(s) 1451, 1820
BstMBI GATC 8 cut(s) 20, 62, 152, 308, 1617, 1764, 1904, 2188
BstMCI CGRYCG 1 cut(s) 882
BstNI CCWGG 1 cut(s) 2033
BstSCI CCNGG 4 cut(s) 116, 137, 457, 2031
BstSFI CTRYAG 7 cut(s) 720, 768, 822, 870, 1509, 1581, 2184
BstSLI GKGCMC 3 cut(s) 123, 1398, 1531
BstUI CGCG 2 cut(s) 674, 2152
BstV2I GAAGAC 2 cut(s) 1101, 1634
BstX2I RGATCY 4 cut(s) 20, 62, 152, 308
BstXI CCANNNNNNTGG 1 cut(s) 2214
BstYI RGATCY 4 cut(s) 20, 62, 152, 308
BsuRI GGCC 4 cut(s) 32, 121, 1368, 2135
BtgI CCRYGG 2 cut(s) 672, 1143
BtgZI GCGATG 1 cut(s) 1098
BtsI GCAGTG 3 cut(s) 1242, 1269, 1473
BtsIMutI CAGTG 7 cut(s) 1242, 1247, 1269, 1390, 1473, 1620, 2069
BtuMI TCGCGA 1 cut(s) 2152
BveI ACCTGC 3 cut(s) 970, 1342, 1975
CaiI CAGNNNCTG 4 cut(s) 935, 1076, 1268, 1463
CciI TCATGA 1 cut(s) 2222
CfoI GCGC 4 cut(s) 451, 512, 625, 761
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 4 cut(s) 119, 120, 2177, 2204
Cfr42I CCGCGG 1 cut(s) 675
Csp6I GTAC 6 cut(s) 246, 1173, 1497, 1503, 1677, 2084
CviAII CATG 2 cut(s) 1864, 2223
CviQI GTAC 6 cut(s) 246, 1173, 1497, 1503, 1677, 2084
DinI GGCGCC 1 cut(s) 624
DpnI GATC 8 cut(s) 22, 64, 154, 310, 1619, 1766, 1906, 2190
DpnII GATC 8 cut(s) 20, 62, 152, 308, 1617, 1764, 1904, 2188
Eam1104I CTCTTC 7 cut(s) 616, 718, 820, 868, 907, 2133, 2152
EarI CTCTTC 7 cut(s) 616, 718, 820, 868, 907, 2133, 2152
Ecl136II GAGCTC 3 cut(s) 711, 813, 861
Eco24I GRGCYC 4 cut(s) 123, 713, 815, 863
Eco31I GGTCTC 1 cut(s) 1451
Eco47I GGWCC 2 cut(s) 2177, 2204
Eco47III AGCGCT 1 cut(s) 760
Eco53kI GAGCTC 3 cut(s) 711, 813, 861
Eco57I CTGAAG 2 cut(s) 1326, 1440
Eco88I CYCGRG 2 cut(s) 922, 1760
EcoICRI GAGCTC 3 cut(s) 711, 813, 861
EcoNI CCTNNNNNAGG 2 cut(s) 846, 984
EcoO109I RGGNCCY 1 cut(s) 2177
EcoRI GAATTC 1 cut(s) 2154
EcoRII CCWGG 1 cut(s) 2031
EcoT38I GRGCYC 4 cut(s) 123, 713, 815, 863
EgeI GGCGCC 1 cut(s) 624
EheI GGCGCC 1 cut(s) 624
FaeI CATG 2 cut(s) 1867, 2226
FaiI YATR 7 cut(s) 1328, 1865, 1886, 1888, 1950, 1967, 2224
FaqI GGGAC 5 cut(s) 85, 409, 568, 1567, 1733
FatI CATG 2 cut(s) 1863, 2222
FauI CCCGC 5 cut(s) 130, 244, 799, 1405, 1444
FriOI GRGCYC 4 cut(s) 123, 713, 815, 863
FspBI CTAG 5 cut(s) 258, 516, 1548, 1814, 1979
GlaI GCGC 4 cut(s) 450, 511, 624, 760
GsaI CCCAGC 2 cut(s) 582, 750
HaeII RGCGCY 3 cut(s) 513, 626, 762
HaeIII GGCC 4 cut(s) 32, 121, 1368, 2135
HapII CCGG 8 cut(s) 117, 138, 372, 378, 458, 507, 878, 1160
HhaI GCGC 4 cut(s) 451, 512, 625, 761
Hin1I GRCGYC 1 cut(s) 623
Hin1II CATG 2 cut(s) 1867, 2226
Hin6I GCGC 4 cut(s) 449, 510, 623, 759
HinP1I GCGC 4 cut(s) 449, 510, 623, 759
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HindIII AAGCTT 2 cut(s) 700, 2162
HinfI GANTC 8 cut(s) 147, 303, 354, 909, 1050, 1213, 1828, 2088
HpaII CCGG 8 cut(s) 117, 138, 372, 378, 458, 507, 878, 1160
HphI GGTGA 2 cut(s) 534, 1999
Hpy166II GTNNAC 2 cut(s) 486, 1529
Hpy188III TCNNGA 9 cut(s) 906, 922, 1135, 1670, 1762, 1913, 2151, 2192, 2223
Hpy8I GTNNAC 2 cut(s) 486, 1529
Hpy99I CGWCG 1 cut(s) 394
HpyAV CCTTC 7 cut(s) 195, 354, 426, 639, 990, 1050, 1620
HpyCH4III ACNGT 5 cut(s) 976, 1132, 1585, 1633, 1742
HpyCH4IV ACGT 2 cut(s) 401, 2049
HpySE526I ACGT 2 cut(s) 401, 2049
Hsp92I GRCGYC 1 cut(s) 623
Hsp92II CATG 2 cut(s) 1867, 2226
HspAI GCGC 4 cut(s) 449, 510, 623, 759
KasI GGCGCC 1 cut(s) 622
KpnI GGTACC 2 cut(s) 249, 1500
KspI CCGCGG 1 cut(s) 675
Kzo9I GATC 8 cut(s) 20, 62, 152, 308, 1617, 1764, 1904, 2188
LguI GCTCTTC 4 cut(s) 718, 820, 868, 907
LweI GCATC 5 cut(s) 119, 500, 962, 1930, 1937
MaeI CTAG 5 cut(s) 258, 516, 1548, 1814, 1979
MaeII ACGT 2 cut(s) 401, 2049
MaeIII GTNAC 3 cut(s) 1132, 1210, 1987
MalI GATC 8 cut(s) 22, 64, 154, 310, 1619, 1766, 1906, 2190
MbiI CCGCTC 3 cut(s) 173, 734, 1807
MboI GATC 8 cut(s) 20, 62, 152, 308, 1617, 1764, 1904, 2188
MflI RGATCY 4 cut(s) 20, 62, 152, 308
MhlI GDGCHC 8 cut(s) 123, 713, 815, 863, 1018, 1241, 1398, 1531
MluCI AATT 9 cut(s) 13, 1604, 1685, 1821, 2003, 2007, 2042, 2154, 2195
Mly113I GGCGCC 1 cut(s) 623
MlyI GAGTC 4 cut(s) 141, 312, 1207, 1822
MmeI TCCRAC 3 cut(s) 243, 286, 704
MseI TTAA 4 cut(s) 1820, 2006, 2093, 2114
MspA1I CMGCKG 6 cut(s) 674, 821, 993, 1076, 1280, 1346
MspI CCGG 8 cut(s) 117, 138, 372, 378, 458, 507, 878, 1160
MspR9I CCNGG 4 cut(s) 118, 139, 459, 2033
Mva1269I GAATGC 3 cut(s) 740, 794, 1663
MvaI CCWGG 1 cut(s) 2033
MvnI CGCG 2 cut(s) 674, 2152
NarI GGCGCC 1 cut(s) 623
NciI CCSGG 3 cut(s) 118, 139, 459
NdeII GATC 8 cut(s) 20, 62, 152, 308, 1617, 1764, 1904, 2188
NlaIII CATG 2 cut(s) 1867, 2226
NmeAIII GCCGAG 4 cut(s) 8, 515, 569, 1007
NmuCI GTSAC 3 cut(s) 1132, 1210, 1987
NruI TCGCGA 1 cut(s) 2152
PaeR7I CTCGAG 1 cut(s) 1760
PagI TCATGA 1 cut(s) 2222
PaqCI CACCTGC 3 cut(s) 970, 1342, 1975
PciSI GCTCTTC 4 cut(s) 718, 820, 868, 907
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PctI GAATGC 3 cut(s) 740, 794, 1663
PfeI GAWTC 4 cut(s) 354, 909, 1050, 2088
PleI GAGTC 4 cut(s) 141, 311, 1207, 1822
PluTI GGCGCC 1 cut(s) 626
PpsI GAGTC 4 cut(s) 141, 311, 1207, 1822
PpuMI RGGWCCY 1 cut(s) 2177
PshBI ATTAAT 1 cut(s) 2006
Psp124BI GAGCTC 3 cut(s) 713, 815, 863
Psp5II RGGWCCY 1 cut(s) 2177
Psp6I CCWGG 1 cut(s) 2031
PspFI CCCAGC 2 cut(s) 578, 746
PspGI CCWGG 1 cut(s) 2031
PspOMI GGGCCC 1 cut(s) 119
PspPI GGNCC 4 cut(s) 119, 120, 2177, 2204
PspPPI RGGWCCY 1 cut(s) 2177
PstI CTGCAG 6 cut(s) 724, 772, 826, 874, 1513, 2188
PstNI CAGNNNCTG 4 cut(s) 935, 1076, 1268, 1463
PsuI RGATCY 4 cut(s) 20, 62, 152, 308
PvuII CAGCTG 1 cut(s) 1280
RruI TCGCGA 1 cut(s) 2152
RsaI GTAC 6 cut(s) 247, 1174, 1498, 1504, 1678, 2085
RsaNI GTAC 6 cut(s) 246, 1173, 1497, 1503, 1677, 2084
SacI GAGCTC 3 cut(s) 713, 815, 863
SacII CCGCGG 1 cut(s) 675
SapI GCTCTTC 4 cut(s) 718, 820, 868, 907
SaqAI TTAA 4 cut(s) 1820, 2006, 2093, 2114
Sau3AI GATC 8 cut(s) 20, 62, 152, 308, 1617, 1764, 1904, 2188
Sau96I GGNCC 4 cut(s) 119, 120, 2177, 2204
ScaI AGTACT 1 cut(s) 1174
SchI GAGTC 4 cut(s) 141, 312, 1207, 1822
ScrFI CCNGG 4 cut(s) 118, 139, 459, 2033
SduI GDGCHC 8 cut(s) 123, 713, 815, 863, 1018, 1241, 1398, 1531
SfaNI GCATC 5 cut(s) 119, 500, 962, 1930, 1937
SfcI CTRYAG 7 cut(s) 720, 768, 822, 870, 1509, 1581, 2184
SfoI GGCGCC 1 cut(s) 624
Sfr274I CTCGAG 1 cut(s) 1760
Sfr303I CCGCGG 1 cut(s) 675
SgrBI CCGCGG 1 cut(s) 675
SinI GGWCC 2 cut(s) 2177, 2204
SlaI CTCGAG 1 cut(s) 1760
SmlI CTYRAG 1 cut(s) 1760
SmoI CTYRAG 1 cut(s) 1760
SpeI ACTAGT 1 cut(s) 1547
Sse9I AATT 9 cut(s) 13, 1604, 1685, 1821, 2003, 2007, 2042, 2154, 2195
SspDI GGCGCC 1 cut(s) 622
SspMI CTAG 5 cut(s) 258, 516, 1548, 1814, 1979
SstI GAGCTC 3 cut(s) 713, 815, 863
StyD4I CCNGG 4 cut(s) 116, 137, 457, 2031
TaaI ACNGT 5 cut(s) 976, 1132, 1585, 1633, 1742
TaiI ACGT 2 cut(s) 404, 2052
TaqI TCGA 5 cut(s) 809, 857, 1761, 1767, 1845
TasI AATT 9 cut(s) 13, 1604, 1685, 1821, 2003, 2007, 2042, 2154, 2195
TatI WGTACW 1 cut(s) 1172
TauI GCSGC 7 cut(s) 623, 628, 677, 1064, 1076, 1349, 1810
TfiI GAWTC 4 cut(s) 354, 909, 1050, 2088
Tru1I TTAA 4 cut(s) 1820, 2006, 2093, 2114
Tru9I TTAA 4 cut(s) 1820, 2006, 2093, 2114
TscAI CASTG 7 cut(s) 1249, 1254, 1269, 1390, 1480, 1627, 2076
TseFI GTSAC 3 cut(s) 1132, 1210, 1987
Tsp45I GTSAC 3 cut(s) 1132, 1210, 1987
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 7 cut(s) 1249, 1254, 1269, 1390, 1480, 1627, 2076
VneI GTGCAC 1 cut(s) 1527
VpaK11BI GGWCC 2 cut(s) 2177, 2204
VspI ATTAAT 1 cut(s) 2006
XagI CCTNNNNNAGG 2 cut(s) 846, 984
XapI RAATTY 2 cut(s) 1685, 2154
XhoI CTCGAG 1 cut(s) 1760
XspI CTAG 5 cut(s) 258, 516, 1548, 1814, 1979
ZrmI AGTACT 1 cut(s) 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.