Rw1G017820

Nsp1-like C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0013006
Physical Location & Seq
Forward (+)
39500309 .. 39505863
5555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G017820.1

Sequence Viewer

Length: 2130 bp
ATGTCGGGTTTCAATTTCGGATCCTCCGCGGCCTCCGCTTCACAATCCACTTCCCCTTTCGGATCCTCCACCCCGCCTTTCTCATTCGCCTCCTCCCCTTCCCTCTTCGGATCCTCCGCGCCCGCCACCGCTTCCACCGGGTTCGGACTCGGATCCTCCTCTTTCGGCTCCGCTTCCTCAAACCCCTCCACCTCCTCCTCCCCTTTCAGCTTCTTATCCAACACCGCCTCCTCCCCCGCTTTCAGTAACCCAAACCCTAGCCCCGCCCCGAGCCCGAACCCATTTCCCTCATTCGGAGTCGGATCCTCCACCGCCTCAACCACCACGGCCGCCGCAGCTTCTACACCTTTCGGATTCAATCCCTCCACCGCTCCGGGTTCTACCCCATCCTTATTCGGGTCATCCGCATTCGGGTCAACTGCATCTGCCTCCGCTTCCGGCACTACCAGCTCTCTGTTCGGCTCGGCTTCGGCGGCTTCACCTTTGTTTGGTACTACTACTACCACTACTTCTTTCGCTCCCAGCACAGGCTCAGCTCTGTTTGGCTCATCCTCTTCATCGGCGGCGCCGCCCTCCTCCACCGCTCAGAACTTGTTTGGCTCTGCTCCGTCGTCCGCGGCTCCAACCACTCCCTCATTCCCAAGCTTTTCGAGCTCTTCCTCTGCAGCCCCAACCACTCCTGCATTCTCCAGCTTTTTGGGCTCTTCGTCTGCAGCATCAACCACTCCCACTCCCGCATTCCCATCCTTTTCGAGCTCTTCGCTGCACTCTTCTTCTGCAGCTCCGGCCACTTCCTCGTTCGCAAGCTCTTCGGGGTTCTCACCATTCCCGAGTTCAGCTCCTGCTAAGCCCACAGCAGCATCCACACCTACTACTACGGTGCCTTCATCAAGCAGCGGTTTCAACCTCTCATTTGGAACGCCCACATCCTCGGCTTCTCAACCTTCTTTTGGATTCAGCAATGCCGCCTCATCAGCCGCAGCGGCTTCCGCCGCATTGTCATTTTCAAAGTCTTCATCGCCATTGTTTTCAACCGTCACCACCACCACGGCTTCTTCAACTCCGGCTGCAAGTAGTACTACACAAGCTCCTTTCTCTTTGCCGGCTTTCAATGTGACTCCTACTGCTCCTCCTGCTGCGAGCACCACTGCACTGACTTCAACGGCAGTGCCTGCCAGCTCAGCTGCTGCTTCATCAAGCACTACTACGAGTTCCTTTACAGGTTTCTCCTTAACACCTGCTTCTTCGGCGGCTACTGCTTCTTCTTCTGGCCTTTCATTGTTTTCCAGTGCCTCTGTGCCCGCCTCATCATCACAAGCACAACCAAGCACCACTCTGCCCGCATTTGGCCTCACTACTTCAGCAACTGCAACTACCACTGCAACTACAACGACGACTGGTACCAGTACCCCTGCTGCTCCGACATCAAGTGCACTTGCTGTGGCTTCTACTAGTGGGACAACCTCAAATGTTAATGGCAGCACTACAGTATCTACAACAACACCAAAATTACCTTCTGAGATCACTGGGAAGACTGTTGAGGAGATTATCAAGGAGTGGAATGCTGAGCTTCAAGAGCGTACTGGAAAATTCCGAAAGCAGGCTAATGCAATAGCAGACTGGGACAGGAGGATTTTGCAGAACCGTGATGTTCTTCTTAGGCTCGAGATCGAAGTGGCAAATGTGGTAGATACCCAAGCTAAGGTGGAGCGGCAACTAGAGTTAATTGAGACTCATCAGCAACAGGTCGATAAGGCTTTACAAAGCATGGAACAAGAAGCTGAGCGTATATACAAGGATGAGCGTGGATCGCTTCTTGATGATGAAGCTGCATCCACAAGAGATGCAATGTATGAGCAGGCTGAGTTTATAGAGAGGGAGCTAGAGCAGGTGACAGAGCAAATCAAATCAATTATTGAGACTGTAAATGCCAACCAGGGAGGAGAATTGGAAGTAAATGATGGGATGGCTCCACTAGATGTGGTTGTACGAATCTTAAACAACCAACTAAGCTCTTTAATGTGGATTGATGAAAAGGCCGAAGAGTTCTCATCGCGAATTCAGAAGCTTGCCAATCAAGGTCCTGCTGCAGATCGTGAATTGATGGGTCCAAGATACTGGATGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0003674 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005642 GO:0005643 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0008092 GO:0008104 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008285 GO:0008289 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010648 GO:0010928 GO:0010930 GO:0010941 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016043 GO:0016070 GO:0017038 GO:0017056 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019894 GO:0019904 GO:0019953 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030159 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032182 GO:0032386 GO:0032501 GO:0032504 GO:0032774 GO:0032879 GO:0032880 GO:0032947 GO:0032991 GO:0033036 GO:0033157 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0042127 GO:0042169 GO:0042175 GO:0042306 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043123 GO:0043130 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044613 GO:0044703 GO:0045184 GO:0045893 GO:0045935 GO:0046483 GO:0046822 GO:0046907 GO:0046966 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051223 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051259 GO:0051291 GO:0051427 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0060255 GO:0060341 GO:0060548 GO:0065003 GO:0065007 GO:0070013 GO:0070201 GO:0070206 GO:0070208 GO:0070727 GO:0071166 GO:0071426 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0098589 GO:0098805 GO:1900180 GO:1901360 GO:1901362 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1903827 GO:1904589 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nsp1_C Nucleoporin_FG

Protein Analysis

709

Amino Acids

71.03

Weight (kDa)

4.74

Isoelectric Point (pI)

59.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nsp1_C PF05064 499 - 602 2.4e-21 Nsp1-like C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45000
fragaria_vesca FvH4_7g10470 FvH4_7g10470 FvH4_7g10470
malus_domestica MD07G1110700.v1.1
prunus_persica Prupe.2G134200_v2.0.a1 Prupe.2G134200_v2.0.a1
pyrus_communis pycom07g09560
rosa_chinensis RchiOBHm_Chr1g0348261
rosa_laevigata RLG00000028672 RLG00000028676
rosa_roxburghii Rroxscaffold_4G00307070
rosa_rugosa Rorug01G0194000
rosa_samantha Rh1AG211000 Rh1BG177500 Rh1CG195400 Rh1DG207000
rosa_wichuraiana Rw1G017820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1246, 1879
Acc36I ACCTGC 2 cut(s) 1246, 1879
Acc65I GGTACC 1 cut(s) 1400
AccB1I GGYRCC 3 cut(s) 565, 880, 1400
AccBSI CCGCTC 3 cut(s) 371, 584, 1711
AccII CGCG 4 cut(s) 29, 119, 617, 2056
AcoI YGGCCR 2 cut(s) 327, 786
AcsI RAATTY 2 cut(s) 1589, 2058
AcuI CTGAAG 1 cut(s) 1344
AcyI GRCGYC 1 cut(s) 566
AfaI GTAC 6 cut(s) 493, 1078, 1402, 1408, 1582, 1989
AfiI CCNNNNNNNGG 9 cut(s) 293, 396, 411, 488, 527, 950, 1346, 1600, 1702
AgsI TTSAA 9 cut(s) 13, 358, 904, 1008, 1032, 1059, 1111, 1161, 1574
AhlI ACTAGT 1 cut(s) 1451
AjnI CCWGG 1 cut(s) 1935
AjuI GAANNNNNNNTTGG 2 cut(s) 1498, 1530
Alw21I GWGCWC 4 cut(s) 656, 758, 1145, 1435
Alw26I GTCTC 2 cut(s) 1724, 1913
Alw44I GTGCAC 1 cut(s) 1431
AlwNI CAGNNNCTG 4 cut(s) 842, 1172, 1187, 1367
Ama87I CYCGRG 3 cut(s) 268, 829, 1664
AoxI GGCC 6 cut(s) 30, 327, 786, 1270, 1348, 2037
ApaLI GTGCAC 1 cut(s) 1431
ApoI RAATTY 2 cut(s) 1589, 2058
Asp718I GGTACC 1 cut(s) 1400
AspLEI GCGC 2 cut(s) 121, 568
AspS9I GGNCC 2 cut(s) 2081, 2108
AsuC2I CCSGG 2 cut(s) 139, 375
AsuHPI GGTGA 4 cut(s) 471, 813, 1030, 1903
AvaI CYCGRG 3 cut(s) 268, 829, 1664
AvaII GGWCC 2 cut(s) 2081, 2108
BaeGI GKGCMC 2 cut(s) 1302, 1435
BaeI ACNNNNGTAYC 2 cut(s) 1473, 1506
BamHI GGATCC 5 cut(s) 20, 62, 110, 152, 302
BanI GGYRCC 3 cut(s) 565, 880, 1400
BanII GRGCYC 4 cut(s) 275, 656, 704, 758
BbsI GAAGAC 2 cut(s) 1005, 1538
Bbv12I GWGCWC 4 cut(s) 656, 758, 1145, 1435
BccI CCATC 5 cut(s) 394, 751, 1955, 1960, 2098
BceAI ACGGC 3 cut(s) 342, 1065, 1179
BcgI CGANNNNNNTGC 2 cut(s) 792, 826
BciT130I CCWGG 1 cut(s) 1937
BcnI CCSGG 2 cut(s) 139, 375
BcoDI GTCTC 2 cut(s) 1724, 1913
BcuI ACTAGT 1 cut(s) 1451
BfaI CTAG 5 cut(s) 258, 1452, 1718, 1883, 1976
BfmI CTRYAG 5 cut(s) 663, 711, 777, 1485, 2088
BfoI RGCGCY 1 cut(s) 569
BfuAI ACCTGC 2 cut(s) 1246, 1879
BglI GCCNNNNNGGC 1 cut(s) 983
BlpI GCTNAGC 5 cut(s) 532, 846, 1180, 1566, 1782
BmcAI AGTACT 1 cut(s) 1078
Bme1390I CCNGG 3 cut(s) 139, 375, 1937
Bme18I GGWCC 2 cut(s) 2081, 2108
BmeT110I CYCGRG 3 cut(s) 268, 829, 1664
BmgT120I GGNCC 2 cut(s) 2081, 2108
BmrFI CCNGG 3 cut(s) 139, 375, 1937
BmrI ACTGGG 2 cut(s) 1536, 1630
BmsI GCATC 5 cut(s) 431, 725, 869, 1834, 1841
BmuI ACTGGG 2 cut(s) 1536, 1630
BpiI GAAGAC 2 cut(s) 1005, 1538
BplI GAGNNNNNCTC 2 cut(s) 823, 855
BpmI CTGGAG 1 cut(s) 673
Bpu10I CCTNAGC 1 cut(s) 1701
Bpu1102I GCTNAGC 5 cut(s) 532, 846, 1180, 1566, 1782
BpuMI CCSGG 2 cut(s) 139, 375
BsaHI GRCGYC 1 cut(s) 566
BsaJI CCNNGG 6 cut(s) 27, 324, 615, 930, 1047, 1936
BsaXI ACNNNNNCTCC 8 cut(s) 212, 242, 715, 745, 1072, 1102, 1114, 1144
Bsc4I CCNNNNNNNGG 9 cut(s) 293, 396, 411, 488, 527, 950, 1346, 1600, 1702
Bse118I RCCGGY 1 cut(s) 1102
Bse1I ACTGG 7 cut(s) 1287, 1402, 1404, 1531, 1588, 1625, 2123
Bse3DI GCAATG 2 cut(s) 967, 1854
BseBI CCWGG 1 cut(s) 1937
BseDI CCNNGG 6 cut(s) 27, 324, 615, 930, 1047, 1936
BseLI CCNNNNNNNGG 9 cut(s) 293, 396, 411, 488, 527, 950, 1346, 1600, 1702
BseMI GCAATG 2 cut(s) 967, 1854
BseMII CTCAG 7 cut(s) 546, 599, 1194, 1509, 1557, 1773, 1854
BseNI ACTGG 7 cut(s) 1287, 1402, 1404, 1531, 1588, 1625, 2123
BseRI GAGGAG 9 cut(s) 82, 148, 184, 187, 220, 565, 1119, 1556, 1956
BseSI GKGCMC 2 cut(s) 1302, 1435
BseX3I CGGCCG 1 cut(s) 327
BseYI CCCAGC 1 cut(s) 521
BsgI GTGCAG 2 cut(s) 749, 1134
Bsh1236I CGCG 4 cut(s) 29, 119, 617, 2056
Bsh1285I CGRYCG 1 cut(s) 330
BshFI GGCC 6 cut(s) 32, 329, 788, 1272, 1350, 2039
BshNI GGYRCC 3 cut(s) 565, 880, 1400
BsiEI CGRYCG 1 cut(s) 330
BsiHKAI GWGCWC 4 cut(s) 656, 758, 1145, 1435
BsiHKCI CYCGRG 3 cut(s) 268, 829, 1664
BsiSI CCGG 6 cut(s) 138, 374, 438, 785, 1064, 1103
BslFI GGGAC 2 cut(s) 1471, 1637
BslI CCNNNNNNNGG 9 cut(s) 293, 396, 411, 488, 527, 950, 1346, 1600, 1702
BsmAI GTCTC 2 cut(s) 1724, 1913
BsmFI GGGAC 2 cut(s) 1471, 1637
BsmI GAATGC 4 cut(s) 407, 683, 737, 1567
BsnI GGCC 6 cut(s) 32, 329, 788, 1272, 1350, 2039
BsoBI CYCGRG 3 cut(s) 268, 829, 1664
Bsp1286I GDGCHC 7 cut(s) 275, 656, 704, 758, 1145, 1302, 1435
Bsp143I GATC 9 cut(s) 20, 62, 110, 152, 302, 1521, 1668, 1808, 2092
Bsp1720I GCTNAGC 5 cut(s) 532, 846, 1180, 1566, 1782
Bsp68I TCGCGA 1 cut(s) 2056
BspANI GGCC 6 cut(s) 32, 329, 788, 1272, 1350, 2039
BspCNI CTCAG 7 cut(s) 545, 598, 1193, 1510, 1558, 1774, 1855
BspFNI CGCG 4 cut(s) 29, 119, 617, 2056
BspHI TCATGA 1 cut(s) 2126
BspMAI CTGCAG 4 cut(s) 667, 715, 781, 2092
BspMI ACCTGC 2 cut(s) 1246, 1879
BspQI GCTCTTC 4 cut(s) 661, 709, 763, 814
BspT107I GGYRCC 3 cut(s) 565, 880, 1400
BsrBI CCGCTC 3 cut(s) 371, 584, 1711
BsrDI GCAATG 2 cut(s) 967, 1854
BsrFI RCCGGY 1 cut(s) 1102
BsrI ACTGG 7 cut(s) 1287, 1402, 1404, 1531, 1588, 1625, 2123
BssAI RCCGGY 1 cut(s) 1102
BssECI CCNNGG 6 cut(s) 27, 324, 615, 930, 1047, 1936
BssMI GATC 9 cut(s) 20, 62, 110, 152, 302, 1521, 1668, 1808, 2092
BssNI GRCGYC 1 cut(s) 566
Bst2UI CCWGG 1 cut(s) 1937
Bst4CI ACNGT 6 cut(s) 880, 1036, 1489, 1537, 1646, 1924
Bst6I CTCTTC 8 cut(s) 110, 559, 661, 709, 763, 775, 814, 2037
BstACI GRCGYC 1 cut(s) 566
BstAPI GCANNNNNTGC 1 cut(s) 1172
BstDSI CCRYGG 4 cut(s) 27, 324, 615, 1047
BstFNI CGCG 4 cut(s) 29, 119, 617, 2056
BstH2I RGCGCY 1 cut(s) 569
BstHHI GCGC 2 cut(s) 121, 568
BstKTI GATC 9 cut(s) 23, 65, 113, 155, 305, 1524, 1671, 1811, 2095
BstMAI GTCTC 2 cut(s) 1724, 1913
BstMBI GATC 9 cut(s) 20, 62, 110, 152, 302, 1521, 1668, 1808, 2092
BstMCI CGRYCG 1 cut(s) 330
BstNI CCWGG 1 cut(s) 1937
BstSCI CCNGG 3 cut(s) 137, 373, 1935
BstSFI CTRYAG 5 cut(s) 663, 711, 777, 1485, 2088
BstSLI GKGCMC 2 cut(s) 1302, 1435
BstUI CGCG 4 cut(s) 29, 119, 617, 2056
BstV2I GAAGAC 2 cut(s) 1005, 1538
BstX2I RGATCY 5 cut(s) 20, 62, 110, 152, 302
BstXI CCANNNNNNTGG 2 cut(s) 697, 2118
BstYI RGATCY 5 cut(s) 20, 62, 110, 152, 302
BstZI CGGCCG 1 cut(s) 327
BsuRI GGCC 6 cut(s) 32, 329, 788, 1272, 1350, 2039
BtgI CCRYGG 4 cut(s) 27, 324, 615, 1047
BtgZI GCGATG 2 cut(s) 1002, 2037
BtsI GCAGTG 3 cut(s) 1146, 1173, 1377
BtsIMutI CAGTG 6 cut(s) 1146, 1151, 1173, 1294, 1377, 1524
BtuMI TCGCGA 1 cut(s) 2056
BveI ACCTGC 2 cut(s) 1246, 1879
CaiI CAGNNNCTG 4 cut(s) 842, 1172, 1187, 1367
CciI TCATGA 1 cut(s) 2126
CfoI GCGC 2 cut(s) 121, 568
Cfr10I RCCGGY 1 cut(s) 1102
Cfr13I GGNCC 2 cut(s) 2081, 2108
Cfr42I CCGCGG 2 cut(s) 30, 618
Csp6I GTAC 6 cut(s) 492, 1077, 1401, 1407, 1581, 1988
CviAII CATG 2 cut(s) 1768, 2127
CviQI GTAC 6 cut(s) 492, 1077, 1401, 1407, 1581, 1988
DinI GGCGCC 1 cut(s) 567
DpnI GATC 9 cut(s) 22, 64, 112, 154, 304, 1523, 1670, 1810, 2094
DpnII GATC 9 cut(s) 20, 62, 110, 152, 302, 1521, 1668, 1808, 2092
EaeI YGGCCR 2 cut(s) 327, 786
EagI CGGCCG 1 cut(s) 327
Eam1104I CTCTTC 8 cut(s) 110, 559, 661, 709, 763, 775, 814, 2037
EarI CTCTTC 8 cut(s) 110, 559, 661, 709, 763, 775, 814, 2037
EciI GGCGGA 1 cut(s) 979
Ecl136II GAGCTC 2 cut(s) 654, 756
EclXI CGGCCG 1 cut(s) 327
Eco24I GRGCYC 4 cut(s) 275, 656, 704, 758
Eco47I GGWCC 2 cut(s) 2081, 2108
Eco52I CGGCCG 1 cut(s) 327
Eco53kI GAGCTC 2 cut(s) 654, 756
Eco57I CTGAAG 1 cut(s) 1344
Eco88I CYCGRG 3 cut(s) 268, 829, 1664
EcoICRI GAGCTC 2 cut(s) 654, 756
EcoO109I RGGNCCY 1 cut(s) 2081
EcoRI GAATTC 1 cut(s) 2058
EcoRII CCWGG 1 cut(s) 1935
EcoT38I GRGCYC 4 cut(s) 275, 656, 704, 758
EgeI GGCGCC 1 cut(s) 567
EheI GGCGCC 1 cut(s) 567
FaeI CATG 2 cut(s) 1771, 2130
FaiI YATR 6 cut(s) 1769, 1790, 1792, 1854, 1871, 2128
FaqI GGGAC 2 cut(s) 1471, 1637
FatI CATG 2 cut(s) 1767, 2126
FauI CCCGC 7 cut(s) 81, 130, 244, 271, 742, 1309, 1348
FokI GGATG 9 cut(s) 373, 388, 535, 730, 847, 913, 1811, 1819, 1978
FriOI GRGCYC 4 cut(s) 275, 656, 704, 758
FspBI CTAG 5 cut(s) 258, 1452, 1718, 1883, 1976
GlaI GCGC 2 cut(s) 120, 567
GsaI CCCAGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 673
HaeII RGCGCY 1 cut(s) 569
HaeIII GGCC 6 cut(s) 32, 329, 788, 1272, 1350, 2039
HapII CCGG 6 cut(s) 138, 374, 438, 785, 1064, 1103
HhaI GCGC 2 cut(s) 121, 568
Hin1I GRCGYC 1 cut(s) 566
Hin1II CATG 2 cut(s) 1771, 2130
Hin6I GCGC 2 cut(s) 119, 566
HinP1I GCGC 2 cut(s) 119, 566
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HindIII AAGCTT 2 cut(s) 643, 2066
HinfI GANTC 7 cut(s) 147, 297, 354, 954, 1117, 1732, 1992
HpaII CCGG 6 cut(s) 138, 374, 438, 785, 1064, 1103
HphI GGTGA 4 cut(s) 471, 813, 1030, 1903
Hpy166II GTNNAC 2 cut(s) 417, 1433
Hpy188III TCNNGA 7 cut(s) 829, 1574, 1666, 1817, 2055, 2096, 2127
Hpy8I GTNNAC 2 cut(s) 417, 1433
Hpy99I CGWCG 2 cut(s) 613, 1396
HpyAV CCTTC 4 cut(s) 108, 894, 954, 1524
HpyCH4III ACNGT 6 cut(s) 880, 1036, 1489, 1537, 1646, 1924
Hsp92I GRCGYC 1 cut(s) 566
Hsp92II CATG 2 cut(s) 1771, 2130
HspAI GCGC 2 cut(s) 119, 566
KasI GGCGCC 1 cut(s) 565
KpnI GGTACC 1 cut(s) 1404
KroI GCCGGC 1 cut(s) 1102
KroNI GCCGGC 1 cut(s) 1104
KspI CCGCGG 2 cut(s) 30, 618
Kzo9I GATC 9 cut(s) 20, 62, 110, 152, 302, 1521, 1668, 1808, 2092
LguI GCTCTTC 4 cut(s) 661, 709, 763, 814
LweI GCATC 5 cut(s) 431, 725, 869, 1834, 1841
MaeI CTAG 5 cut(s) 258, 1452, 1718, 1883, 1976
MaeIII GTNAC 4 cut(s) 245, 1036, 1114, 1891
MalI GATC 9 cut(s) 22, 64, 112, 154, 304, 1523, 1670, 1810, 2094
MbiI CCGCTC 3 cut(s) 371, 584, 1711
MboI GATC 9 cut(s) 20, 62, 110, 152, 302, 1521, 1668, 1808, 2092
MflI RGATCY 5 cut(s) 20, 62, 110, 152, 302
MhlI GDGCHC 7 cut(s) 275, 656, 704, 758, 1145, 1302, 1435
MluCI AATT 8 cut(s) 13, 1508, 1589, 1725, 1911, 1946, 2058, 2099
Mly113I GGCGCC 1 cut(s) 566
MlyI GAGTC 4 cut(s) 141, 306, 1111, 1726
MmeI TCCRAC 4 cut(s) 243, 280, 647, 1445
MroNI GCCGGC 1 cut(s) 1102
MseI TTAA 5 cut(s) 1232, 1473, 1724, 1997, 2018
MspA1I CMGCKG 5 cut(s) 29, 617, 897, 983, 1184
MspI CCGG 6 cut(s) 138, 374, 438, 785, 1064, 1103
MspR9I CCNGG 3 cut(s) 139, 375, 1937
Mva1269I GAATGC 4 cut(s) 407, 683, 737, 1567
MvaI CCWGG 1 cut(s) 1937
MvnI CGCG 4 cut(s) 29, 119, 617, 2056
NaeI GCCGGC 1 cut(s) 1104
NarI GGCGCC 1 cut(s) 566
NciI CCSGG 2 cut(s) 139, 375
NdeII GATC 9 cut(s) 20, 62, 110, 152, 302, 1521, 1668, 1808, 2092
NgoMIV GCCGGC 1 cut(s) 1102
NlaIII CATG 2 cut(s) 1771, 2130
NmeAIII GCCGAG 2 cut(s) 443, 911
NmuCI GTSAC 3 cut(s) 1036, 1114, 1891
NruI TCGCGA 1 cut(s) 2056
PaeR7I CTCGAG 1 cut(s) 1664
PagI TCATGA 1 cut(s) 2126
PaqCI CACCTGC 2 cut(s) 1246, 1879
PciSI GCTCTTC 4 cut(s) 661, 709, 763, 814
PctI GAATGC 4 cut(s) 407, 683, 737, 1567
PdiI GCCGGC 1 cut(s) 1104
PfeI GAWTC 3 cut(s) 354, 954, 1992
PleI GAGTC 4 cut(s) 141, 305, 1111, 1726
PluTI GGCGCC 1 cut(s) 569
PpsI GAGTC 4 cut(s) 141, 305, 1111, 1726
PpuMI RGGWCCY 1 cut(s) 2081
Psp124BI GAGCTC 2 cut(s) 656, 758
Psp5II RGGWCCY 1 cut(s) 2081
Psp6I CCWGG 1 cut(s) 1935
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 1 cut(s) 1935
PspPI GGNCC 2 cut(s) 2081, 2108
PspPPI RGGWCCY 1 cut(s) 2081
PstI CTGCAG 4 cut(s) 667, 715, 781, 2092
PstNI CAGNNNCTG 4 cut(s) 842, 1172, 1187, 1367
PsuI RGATCY 5 cut(s) 20, 62, 110, 152, 302
PvuII CAGCTG 1 cut(s) 1184
RruI TCGCGA 1 cut(s) 2056
RsaI GTAC 6 cut(s) 493, 1078, 1402, 1408, 1582, 1989
RsaNI GTAC 6 cut(s) 492, 1077, 1401, 1407, 1581, 1988
SacI GAGCTC 2 cut(s) 656, 758
SacII CCGCGG 2 cut(s) 30, 618
SapI GCTCTTC 4 cut(s) 661, 709, 763, 814
SaqAI TTAA 5 cut(s) 1232, 1473, 1724, 1997, 2018
Sau3AI GATC 9 cut(s) 20, 62, 110, 152, 302, 1521, 1668, 1808, 2092
Sau96I GGNCC 2 cut(s) 2081, 2108
ScaI AGTACT 1 cut(s) 1078
SchI GAGTC 4 cut(s) 141, 306, 1111, 1726
ScrFI CCNGG 3 cut(s) 139, 375, 1937
SduI GDGCHC 7 cut(s) 275, 656, 704, 758, 1145, 1302, 1435
SfaNI GCATC 5 cut(s) 431, 725, 869, 1834, 1841
SfcI CTRYAG 5 cut(s) 663, 711, 777, 1485, 2088
SfoI GGCGCC 1 cut(s) 567
Sfr274I CTCGAG 1 cut(s) 1664
Sfr303I CCGCGG 2 cut(s) 30, 618
SgrBI CCGCGG 2 cut(s) 30, 618
SinI GGWCC 2 cut(s) 2081, 2108
SlaI CTCGAG 1 cut(s) 1664
SmlI CTYRAG 1 cut(s) 1664
SmoI CTYRAG 1 cut(s) 1664
SpeI ACTAGT 1 cut(s) 1451
Sse9I AATT 8 cut(s) 13, 1508, 1589, 1725, 1911, 1946, 2058, 2099
SspDI GGCGCC 1 cut(s) 565
SspMI CTAG 5 cut(s) 258, 1452, 1718, 1883, 1976
SstI GAGCTC 2 cut(s) 656, 758
StyD4I CCNGG 3 cut(s) 137, 373, 1935
TaaI ACNGT 6 cut(s) 880, 1036, 1489, 1537, 1646, 1924
TaqI TCGA 5 cut(s) 650, 752, 1665, 1671, 1749
TasI AATT 8 cut(s) 13, 1508, 1589, 1725, 1911, 1946, 2058, 2099
TatI WGTACW 1 cut(s) 1076
TfiI GAWTC 3 cut(s) 354, 954, 1992
Tru1I TTAA 5 cut(s) 1232, 1473, 1724, 1997, 2018
Tru9I TTAA 5 cut(s) 1232, 1473, 1724, 1997, 2018
TscAI CASTG 6 cut(s) 1153, 1158, 1173, 1294, 1384, 1531
TseFI GTSAC 3 cut(s) 1036, 1114, 1891
Tsp45I GTSAC 3 cut(s) 1036, 1114, 1891
TspDTI ATGAA 7 cut(s) 546, 876, 1005, 1182, 1266, 1839, 2046
TspGWI ACGGA 1 cut(s) 597
TspRI CASTG 6 cut(s) 1153, 1158, 1173, 1294, 1384, 1531
VneI GTGCAC 1 cut(s) 1431
VpaK11BI GGWCC 2 cut(s) 2081, 2108
XapI RAATTY 2 cut(s) 1589, 2058
XhoI CTCGAG 1 cut(s) 1664
XspI CTAG 5 cut(s) 258, 1452, 1718, 1883, 1976
ZrmI AGTACT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.