AT2G46030

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008995
Physical Location & Seq
Reverse (-)
18932019 .. 18934138
2120 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46030.3

Sequence Viewer

Length: 552 bp
ATGGCGTCGCCGAGCAAACGTCGAGAGATGGATATGATGAAACTGATGATGAGTGATTACAAGGTGGATACTGTCAACGACGATTTGCAAATGTTCTATGTCACATTCCATGGACCCACTGACAGTCTTTATCAGGGGGGTGTATGGAAGATAAAAGTGGAACTTCCCGAAGCTTATCCATACAAATCTCCATCTGTTGGATTTGTCAACAAGATTTATCACCCTAATGTCGATGAATCTTCTGGTGCTGTTTGCTTAGATGTTATAAACCAGACTTGGAGCCCAATGTTTGATCTTATAAATGTGTTCGAATCCTTCCTTCCGCAACTGCTTCTATATCCAAACCCATCAGATCCATTTAATGGAGAAGCCGCTTCTCTGTTGATGCGAGACCGTGCAGCCTATGAGCTCAAAGTGAAAGAATATTGTGAGAAATATGCAAAACCAGAGGAGATTTTAAGTGATGATGATGATGATGATAGCATGAGCGAAGATGGTTCGGACTCTGATGATGATGACGACGACGAAATCGTTGGGAAAGCCGATCCATGA

Protein Analysis

183

Amino Acids

20.78

Weight (kDa)

4.12

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 82 - 214 3e-40 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 266, 299
AccB7I CCANNNNNTGG 2 cut(s) 197, 362
AciI CCGC 2 cut(s) 323, 372
AclWI GGATC 2 cut(s) 347, 539
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 197, 362
AluBI AGCT 2 cut(s) 173, 409
AluI AGCT 2 cut(s) 173, 409
Alw21I GWGCWC 1 cut(s) 411
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 2 cut(s) 347, 539
ApeKI GCWGC 1 cut(s) 398
AspS9I GGNCC 1 cut(s) 113
AsuHPI GGTGA 1 cut(s) 212
AsuII TTCGAA 1 cut(s) 309
AvaII GGWCC 1 cut(s) 113
BanII GRGCYC 2 cut(s) 284, 411
Bbv12I GWGCWC 1 cut(s) 411
BbvI GCAGC 1 cut(s) 410
BccI CCATC 4 cut(s) 22, 199, 355, 488
BciVI GTATCC 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 384
BfuI GTATCC 1 cut(s) 61
BisI GCNGC 2 cut(s) 372, 399
BlsI GCNGC 2 cut(s) 373, 400
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 1 cut(s) 113
BmiI GGNNCC 2 cut(s) 115, 281
BmsI GCATC 1 cut(s) 375
Bpu14I TTCGAA 1 cut(s) 309
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 384
BsaJI CCNNGG 1 cut(s) 109
Bsc4I CCNNNNNNNGG 2 cut(s) 197, 362
BseDI CCNNGG 1 cut(s) 109
BseLI CCNNNNNNNGG 2 cut(s) 197, 362
BseRI GAGGAG 1 cut(s) 464
BseXI GCAGC 1 cut(s) 410
BsgI GTGCAG 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 411
BslI CCNNNNNNNGG 2 cut(s) 197, 362
BsmAI GTCTC 1 cut(s) 384
Bso31I GGTCTC 1 cut(s) 384
Bsp119I TTCGAA 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 284, 411
Bsp143I GATC 3 cut(s) 292, 352, 544
Bsp19I CCATGG 1 cut(s) 109
BspACI CCGC 2 cut(s) 323, 372
BspLI GGNNCC 2 cut(s) 115, 281
BspPI GGATC 2 cut(s) 347, 539
BspT104I TTCGAA 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 384
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 3 cut(s) 292, 352, 544
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 109
Bst4CI ACNGT 3 cut(s) 73, 125, 395
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 256
BstDSI CCRYGG 1 cut(s) 109
BstKTI GATC 3 cut(s) 295, 355, 547
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 3 cut(s) 292, 352, 544
BstV1I GCAGC 1 cut(s) 410
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuI GTATCC 1 cut(s) 61
BtgI CCRYGG 1 cut(s) 109
BtsIMutI CAGTG 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 113
CviAII CATG 3 cut(s) 110, 484, 549
CviJI RGCY 6 cut(s) 173, 282, 371, 401, 409, 542
CviKI_1 RGCY 6 cut(s) 173, 282, 371, 401, 409, 542
DdeI CTNAG 1 cut(s) 256
DpnI GATC 3 cut(s) 294, 354, 546
DpnII GATC 3 cut(s) 292, 352, 544
Ecl136II GAGCTC 1 cut(s) 409
Eco130I CCWWGG 1 cut(s) 109
Eco24I GRGCYC 2 cut(s) 284, 411
Eco31I GGTCTC 1 cut(s) 384
Eco47I GGWCC 1 cut(s) 113
Eco53kI GAGCTC 1 cut(s) 409
EcoICRI GAGCTC 1 cut(s) 409
EcoT14I CCWWGG 1 cut(s) 109
EcoT38I GRGCYC 2 cut(s) 284, 411
ErhI CCWWGG 1 cut(s) 109
FaeI CATG 3 cut(s) 113, 487, 552
FalI AAGNNNNNCTT 2 cut(s) 147, 179
FatI CATG 3 cut(s) 109, 483, 548
Fnu4HI GCNGC 2 cut(s) 372, 399
FriOI GRGCYC 2 cut(s) 284, 411
Fsp4HI GCNGC 2 cut(s) 372, 399
GluI GCNGC 2 cut(s) 372, 399
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 113, 487, 552
HincII GTYRAC 2 cut(s) 76, 208
HindII GTYRAC 2 cut(s) 76, 208
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 3 cut(s) 236, 311, 503
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 2 cut(s) 76, 208
Hpy188I TCNGA 3 cut(s) 352, 502, 508
Hpy188III TCNNGA 2 cut(s) 23, 167
Hpy8I GTNNAC 2 cut(s) 76, 208
Hpy99I CGWCG 5 cut(s) 10, 24, 83, 524, 527
HpyAV CCTTC 2 cut(s) 325, 329
HpyCH4III ACNGT 3 cut(s) 73, 125, 395
HpyCH4IV ACGT 1 cut(s) 19
HpyCH4V TGCA 3 cut(s) 88, 398, 440
HpyF3I CTNAG 1 cut(s) 256
HpySE526I ACGT 1 cut(s) 19
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 113, 487, 552
Kzo9I GATC 3 cut(s) 292, 352, 544
LmnI GCTCC 1 cut(s) 279
LpnPI CCDG 4 cut(s) 119, 228, 284, 459
Lsp1109I GCAGC 1 cut(s) 410
LweI GCATC 1 cut(s) 375
MaeII ACGT 1 cut(s) 19
MaeIII GTNAC 1 cut(s) 100
MalI GATC 3 cut(s) 294, 354, 546
MboI GATC 3 cut(s) 292, 352, 544
MboII GAAGA 3 cut(s) 160, 231, 503
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 2 cut(s) 284, 411
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 1 cut(s) 178
MnlI CCTC 1 cut(s) 442
MseI TTAA 2 cut(s) 360, 458
MslI CAYNNNNRTG 1 cut(s) 225
NcoI CCATGG 1 cut(s) 109
NdeII GATC 3 cut(s) 292, 352, 544
NlaIII CATG 3 cut(s) 113, 487, 552
NlaIV GGNNCC 2 cut(s) 115, 281
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 100
NspV TTCGAA 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 528
PfeI GAWTC 2 cut(s) 236, 311
PflMI CCANNNNNTGG 2 cut(s) 197, 362
PkrI GCNGC 2 cut(s) 373, 400
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
PsiI TTATAA 2 cut(s) 266, 299
Psp124BI GAGCTC 1 cut(s) 411
PspN4I GGNNCC 2 cut(s) 115, 281
PspPI GGNCC 1 cut(s) 113
PsuI RGATCY 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 225
SacI GAGCTC 1 cut(s) 411
SaqAI TTAA 2 cut(s) 360, 458
SatI GCNGC 2 cut(s) 372, 399
Sau3AI GATC 3 cut(s) 292, 352, 544
Sau96I GGNCC 1 cut(s) 113
SchI GAGTC 1 cut(s) 497
SduI GDGCHC 2 cut(s) 284, 411
SetI ASST 4 cut(s) 22, 66, 175, 411
SfaNI GCATC 1 cut(s) 375
SfuI TTCGAA 1 cut(s) 309
SinI GGWCC 1 cut(s) 113
SmiMI CAYNNNNRTG 1 cut(s) 225
SsiI CCGC 2 cut(s) 323, 372
SspI AATATT 1 cut(s) 425
SstI GAGCTC 1 cut(s) 411
StyI CCWWGG 1 cut(s) 109
TaaI ACNGT 3 cut(s) 73, 125, 395
TaiI ACGT 1 cut(s) 22
TaqI TCGA 3 cut(s) 22, 231, 309
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 2 cut(s) 236, 311
Tru1I TTAA 2 cut(s) 360, 458
Tru9I TTAA 2 cut(s) 360, 458
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 1 cut(s) 398
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 2 cut(s) 53, 249
TspRI CASTG 1 cut(s) 124
Van91I CCANNNNNTGG 2 cut(s) 197, 362
VpaK11BI GGWCC 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.