AT3G02070

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005983
Physical Location & Seq
Forward (+)
360921 .. 363775
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G02070.2

Sequence Viewer

Length: 660 bp
ATGGGAGACTCTTCGAGTTCAACCTCTTGGAGCAGTAAAAAGGATACGGAAGATGATCGGATGATTGCTTTTATGTTATCTGAAGAGTACTCTAAACTAGACGGTGCGGTCGGCAGACGCCTCTCAAATCTTGCTCCCGTCCCTCATGTTCCACGAATAAATTGTTATATTCCCAACTTAAATGATGCCACCTTGGATCACCAACGCCTTCTTCAAAGGTTAAATGTTTACGGTTTGTGTGAGTTGAAGGTCTCTGGTGATGGAAACTGCCAGTTCCGAGCCCTTTCTGACCAGTTATACCGATCTCCAGAGTACCACAAGCAAGTGAGGAGAGAAGTTGTTAAACAGCTCAAAGAGTGTCGTTCTATGTATGAAAGTTATGTTCCGATGAAATACAAACGATATTACAAGAAGATGGGAAAATTTGGAGAATGGGGAGACCATATTACCCTACAAGCTGCGGCAGATAGGTTTGCAGCAAAGATATGCTTGCTGACATCCTTTAGAGACACTTGTTTCATTGAAATTATACCTCAATACCAAGCACCTAAGGGTGTGTTATGGTTAAGTTTCTGGTCAGAGGTGCATTATAACTCTTTATACGATATTCAAGCTGCTCCAGTTCAGCATAAGCCAAAGAGAAAACATTGGTTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

219

Amino Acids

25.57

Weight (kDa)

9.13

Isoelectric Point (pI)

54.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 86 - 175 9.9e-12 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
AasI GACNNNNNNGTC 1 cut(s) 107
AciI CCGC 2 cut(s) 107, 461
AclWI GGATC 1 cut(s) 204
AcsI RAATTY 1 cut(s) 422
AcuI CTGAAG 1 cut(s) 102
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 2 cut(s) 89, 314
AgsI TTSAA 5 cut(s) 21, 215, 247, 524, 611
AjuI GAANNNNNNNTTGG 2 cut(s) 195, 227
AluBI AGCT 3 cut(s) 349, 458, 614
AluI AGCT 3 cut(s) 349, 458, 614
Alw26I GTCTC 3 cut(s) 256, 432, 501
AlwI GGATC 1 cut(s) 204
ApeKI GCWGC 3 cut(s) 458, 476, 614
ApoI RAATTY 1 cut(s) 422
AsuHPI GGTGA 2 cut(s) 191, 269
AxyI CCTNAGG 1 cut(s) 549
BanII GRGCYC 1 cut(s) 283
BbvI GCAGC 3 cut(s) 445, 488, 601
BccI CCATC 2 cut(s) 254, 409
BciVI GTATCC 1 cut(s) 37
BcoDI GTCTC 3 cut(s) 256, 432, 501
BfaI CTAG 2 cut(s) 98, 658
BfuI GTATCC 1 cut(s) 37
BisI GCNGC 4 cut(s) 459, 462, 477, 615
BlsI GCNGC 4 cut(s) 460, 463, 478, 616
BmcAI AGTACT 1 cut(s) 89
BmsI GCATC 1 cut(s) 175
BpmI CTGGAG 2 cut(s) 291, 603
BsaHI GRCGYC 1 cut(s) 118
BsaI GGTCTC 2 cut(s) 256, 432
BsaJI CCNNGG 1 cut(s) 192
Bse1I ACTGG 3 cut(s) 271, 292, 620
Bse21I CCTNAGG 1 cut(s) 549
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 2 cut(s) 66, 497
BseNI ACTGG 3 cut(s) 271, 292, 620
BseRI GAGGAG 1 cut(s) 343
BseXI GCAGC 3 cut(s) 445, 488, 601
Bsh1285I CGRYCG 1 cut(s) 111
BsiEI CGRYCG 1 cut(s) 111
BslFI GGGAC 1 cut(s) 125
BsmAI GTCTC 3 cut(s) 256, 432, 501
BsmFI GGGAC 1 cut(s) 125
Bso31I GGTCTC 2 cut(s) 256, 432
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 3 cut(s) 55, 196, 302
BspACI CCGC 2 cut(s) 107, 461
BspPI GGATC 1 cut(s) 204
BspTNI GGTCTC 2 cut(s) 256, 432
BsrI ACTGG 3 cut(s) 271, 292, 620
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 3 cut(s) 55, 196, 302
BssNI GRCGYC 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 104, 233
Bst6I CTCTTC 2 cut(s) 16, 78
BstACI GRCGYC 1 cut(s) 118
BstC8I GCNNGC 1 cut(s) 491
BstDEI CTNAG 1 cut(s) 549
BstF5I GGATG 2 cut(s) 66, 497
BstKTI GATC 3 cut(s) 58, 199, 305
BstMAI GTCTC 3 cut(s) 256, 432, 501
BstMBI GATC 3 cut(s) 55, 196, 302
BstMCI CGRYCG 1 cut(s) 111
BstV1I GCAGC 3 cut(s) 445, 488, 601
Bsu36I CCTNAGG 1 cut(s) 549
BsuI GTATCC 1 cut(s) 37
BtsCI GGATG 2 cut(s) 66, 497
Cac8I GCNNGC 1 cut(s) 491
CseI GACGC 1 cut(s) 126
Csp6I GTAC 2 cut(s) 88, 313
CviAII CATG 1 cut(s) 146
CviJI RGCY 5 cut(s) 281, 349, 458, 614, 634
CviKI_1 RGCY 5 cut(s) 281, 349, 458, 614, 634
CviQI GTAC 2 cut(s) 88, 313
DdeI CTNAG 1 cut(s) 549
DpnI GATC 3 cut(s) 57, 198, 304
DpnII GATC 3 cut(s) 55, 196, 302
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
Eam1104I CTCTTC 2 cut(s) 16, 78
EarI CTCTTC 2 cut(s) 16, 78
Eco130I CCWWGG 1 cut(s) 192
Eco24I GRGCYC 1 cut(s) 283
Eco31I GGTCTC 2 cut(s) 256, 432
Eco57I CTGAAG 1 cut(s) 102
Eco81I CCTNAGG 1 cut(s) 549
EcoT14I CCWWGG 1 cut(s) 192
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 1 cut(s) 149
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FaqI GGGAC 1 cut(s) 125
FatI CATG 1 cut(s) 145
Fnu4HI GCNGC 4 cut(s) 459, 462, 477, 615
FokI GGATG 2 cut(s) 73, 484
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 4 cut(s) 459, 462, 477, 615
FspBI CTAG 2 cut(s) 98, 658
GluI GCNGC 4 cut(s) 459, 462, 477, 615
GsuI CTGGAG 2 cut(s) 291, 603
HgaI GACGC 1 cut(s) 126
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 1 cut(s) 149
HinfI GANTC 1 cut(s) 8
HphI GGTGA 2 cut(s) 191, 269
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 6 cut(s) 60, 82, 278, 289, 387, 580
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 2 cut(s) 218, 241
HpyCH4III ACNGT 2 cut(s) 104, 233
HpyCH4V TGCA 2 cut(s) 476, 586
HpyF3I CTNAG 1 cut(s) 549
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 1 cut(s) 149
Kzo9I GATC 3 cut(s) 55, 196, 302
LmnI GCTCC 3 cut(s) 30, 139, 622
LpnPI CCDG 6 cut(s) 240, 284, 305, 321, 559, 633
Lsp1109I GCAGC 3 cut(s) 445, 488, 601
LweI GCATC 1 cut(s) 175
MaeI CTAG 2 cut(s) 98, 658
MalI GATC 3 cut(s) 57, 198, 304
MboI GATC 3 cut(s) 55, 196, 302
MboII GAAGA 5 cut(s) 3, 62, 95, 203, 424
MhlI GDGCHC 1 cut(s) 283
MluCI AATT 3 cut(s) 160, 422, 525
MlyI GAGTC 1 cut(s) 2
MnlI CCTC 6 cut(s) 34, 131, 153, 321, 543, 574
MseI TTAA 4 cut(s) 179, 221, 342, 566
NdeII GATC 3 cut(s) 55, 196, 302
NlaIII CATG 1 cut(s) 149
PkrI GCNGC 4 cut(s) 460, 463, 478, 616
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
PsiI TTATAA 1 cut(s) 591
RsaI GTAC 2 cut(s) 89, 314
RsaNI GTAC 2 cut(s) 88, 313
SaqAI TTAA 4 cut(s) 179, 221, 342, 566
SatI GCNGC 4 cut(s) 459, 462, 477, 615
Sau3AI GATC 3 cut(s) 55, 196, 302
ScaI AGTACT 1 cut(s) 89
SchI GAGTC 1 cut(s) 2
SduI GDGCHC 1 cut(s) 283
SfaNI GCATC 1 cut(s) 175
Sse9I AATT 3 cut(s) 160, 422, 525
SsiI CCGC 2 cut(s) 107, 461
SspMI CTAG 2 cut(s) 98, 658
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 2 cut(s) 104, 233
TaqI TCGA 1 cut(s) 14
TasI AATT 3 cut(s) 160, 422, 525
TatI WGTACW 1 cut(s) 87
TauI GCSGC 1 cut(s) 464
Tru1I TTAA 4 cut(s) 179, 221, 342, 566
Tru9I TTAA 4 cut(s) 179, 221, 342, 566
TseI GCWGC 3 cut(s) 458, 476, 614
TspDTI ATGAA 3 cut(s) 387, 404, 508
TspGWI ACGGA 1 cut(s) 62
XapI RAATTY 1 cut(s) 422
XspI CTAG 2 cut(s) 98, 658
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.