AT3G02630

Fatty acid desaturase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010828
Physical Location & Seq
Forward (+)
562030 .. 564870
2841 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G02630.1

Sequence Viewer

Length: 1191 bp
ATGGCGATGGCTATGGATCGGATCGTCTTCTCCCCTTCATCTTACGTCTATCGTCCTTGTCAAGCTAGGGGATCCAGATCTTCCAGAGTTTCCATGGCTTCTACGATTCGCTCTGCGACTACAGAGGTTACGAATGGAAGGAAACTGTATATCCCTCCCAGAGAGGTGCATGTCCAAGTCAAACATTCAATGCCGCCACAAAAGTTAGAGATCTTTAAGTCTTTAGAAGGATGGGCTGATGAAACCCTGTTGACTTATTTAAAACCTGTTGAGAAATCGTGGCAGCCTACCGATTTTCTTCCCGAACCTGAGTCGGAAGGATTCTATGACCAAGTCAAGGAGCTAAGGGAAAGGTGCAAGGAACTTCCTGATGACTATTTTGTGGTGCTTGTTGGGGATATGATCACTGAAGAAGCACTTCCTACTTACCAGACCATGTTGAACACATTGGATGGTGTTAGGGATGAGACAGGAGCTAGTCCTACTCCTTGGGCTATATGGACCAGGGCTTGGACTGCTGAGGAGAATAGACATGGGGATCTCCTTAACAAGTATCTTTATCTGTCTGGTCGGGTAGACATGAGGCAGATTGAAAAGACTATTCAGTACCTGATTGGTTCTGGAATGGATCCAAAAACTGAAAACAACCCTTACTTGGGTTTCATCTACACATCATTTCAAGAAAGAGCGACCTTCATCTCCCATGGAAACACTGCCAGACTGGCGAAAGATCGTGGAGATTTGAAACTTGCGCAGATATGCGGGACCATTGCTGCTGATGAGAGGCGTCATGAGACTGCTTACACCAAGATTGTAGAGAAGCTCTTTGAAATTGACCCTGATGGCACGATCTTGGGCCTGGCTGATATGATGAAGAAAAAGATATCAATGCCTGCACATTTAATGTATGATGGCCAAGATGATAACCTGTTTGAGCACTTCTCAACCGTTGCCCAGAGGCTCGGTGTCTACACTGCCAAGGACTATGCTGATATTCTGGAGTTTCTTGTTGAACGGTGGAATGTGGAGACTTTGACAGACCTTTCTAGTGAAGGACACAGGGCCCAGGACTTTGTCTGCGGACTACCTGCAAGAATCCGTAAAATTGAAGAGAGAGCTCAAGGAAGAGCCAAAGAAGCTGCCAAAAACATACCATTCAGCTGGATATTTGGTCGAAATATCAGGGCTTAA

Protein Analysis

396

Amino Acids

45.3

Weight (kDa)

5.87

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 67 - 390 2.9e-160 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02630 AT5G16230 AT5G16240
fragaria_vesca FvH4_6g00080
malus_domestica MD00G1017300.v1.1 MD12G1261600.v1.1
prunus_persica Prupe.6G365100_v2.0.a1 Prupe.6G365100_v2.0.a1 Prupe.6G365200_v2.0.a1
pyrus_communis pycom04g21990
rosa_chinensis RchiOBHm_Chr3g0447411
rosa_laevigata RLG00000026001
rosa_multiflora Rmu_ssc0000473.1_g000020
rosa_roxburghii Rroxscaffold_6G00425610
rosa_rugosa Rorug02G0601300
rosa_samantha Rh3AG001400 Rh3BG001100 Rh3CG001000 Rh3DG001300
rosa_wichuraiana Rw3G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 753
Acc36I ACCTGC 1 cut(s) 1096
AccB7I CCANNNNNTGG 1 cut(s) 510
AccI GTMKAC 2 cut(s) 576, 969
AciI CCGC 3 cut(s) 194, 762, 1080
AclWI GGATC 7 cut(s) 24, 29, 66, 79, 546, 623, 636
AcoI YGGCCR 1 cut(s) 913
AcuI CTGAAG 1 cut(s) 429
AcyI GRCGYC 1 cut(s) 787
AfaI GTAC 1 cut(s) 608
AfiI CCNNNNNNNGG 4 cut(s) 337, 510, 655, 656
AgsI TTSAA 8 cut(s) 189, 442, 593, 680, 745, 830, 1013, 1109
AjnI CCWGG 3 cut(s) 503, 858, 1065
AluBI AGCT 7 cut(s) 65, 343, 476, 823, 1118, 1139, 1161
AluI AGCT 7 cut(s) 65, 343, 476, 823, 1118, 1139, 1161
Alw21I GWGCWC 2 cut(s) 939, 1120
Alw26I GTCTC 3 cut(s) 461, 788, 1022
AlwI GGATC 7 cut(s) 24, 29, 66, 79, 546, 623, 636
AlwNI CAGNNNCTG 1 cut(s) 610
AoxI GGCC 3 cut(s) 856, 913, 1062
ApaI GGGCCC 1 cut(s) 1066
ApeKI GCWGC 3 cut(s) 283, 773, 1139
Asp700I GAANNNNTTC 1 cut(s) 417
AspLEI GCGC 1 cut(s) 754
AspS9I GGNCC 5 cut(s) 501, 765, 856, 1062, 1063
AvaII GGWCC 2 cut(s) 501, 765
BaeGI GKGCMC 1 cut(s) 1066
BalI TGGCCA 1 cut(s) 915
BamHI GGATCC 2 cut(s) 71, 628
BanII GRGCYC 2 cut(s) 1066, 1120
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 2 cut(s) 939, 1120
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 3 cut(s) 295, 760, 1126
BccI CCATC 4 cut(s) 225, 446, 836, 905
BciT130I CCWGG 3 cut(s) 505, 860, 1067
BclI TGATCA 1 cut(s) 402
BcoDI GTCTC 3 cut(s) 461, 788, 1022
BfaI CTAG 3 cut(s) 66, 477, 1047
BfmI CTRYAG 1 cut(s) 120
BfuAI ACCTGC 1 cut(s) 1096
BglI GCCNNNNNGGC 1 cut(s) 722
BglII AGATCT 2 cut(s) 77, 210
BisI GCNGC 4 cut(s) 194, 284, 774, 1140
BlsI GCNGC 4 cut(s) 195, 285, 775, 1141
Bme1390I CCNGG 3 cut(s) 505, 860, 1067
Bme18I GGWCC 2 cut(s) 501, 765
BmgT120I GGNCC 5 cut(s) 501, 765, 856, 1062, 1063
BmiI GGNNCC 4 cut(s) 73, 630, 766, 1064
BmrFI CCNGG 3 cut(s) 505, 860, 1067
BpiI GAAGAC 1 cut(s) 19
BplI GAGNNNNNCTC 2 cut(s) 926, 958
BpmI CTGGAG 1 cut(s) 1019
Bpu10I CCTNAGC 2 cut(s) 344, 519
BpuEI CTTGAG 1 cut(s) 1104
BsaBI GATNNNNATC 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 787
BsaJI CCNNGG 6 cut(s) 93, 488, 504, 703, 978, 1065
Bsc4I CCNNNNNNNGG 4 cut(s) 337, 510, 655, 656
Bse1I ACTGG 1 cut(s) 726
Bse3DI GCAATG 1 cut(s) 768
Bse8I GATNNNNATC 1 cut(s) 76
BseBI CCWGG 3 cut(s) 505, 860, 1067
BseDI CCNNGG 6 cut(s) 93, 488, 504, 703, 978, 1065
BseGI GGATG 3 cut(s) 236, 457, 469
BseJI GATNNNNATC 1 cut(s) 76
BseLI CCNNNNNNNGG 4 cut(s) 337, 510, 655, 656
BseMI GCAATG 1 cut(s) 768
BseMII CTCAG 2 cut(s) 300, 510
BseNI ACTGG 1 cut(s) 726
BseRI GAGGAG 1 cut(s) 536
BseSI GKGCMC 1 cut(s) 1066
BseXI GCAGC 3 cut(s) 295, 760, 1126
BsgI GTGCAG 1 cut(s) 879
BshFI GGCC 3 cut(s) 858, 915, 1064
BsiHKAI GWGCWC 2 cut(s) 939, 1120
BslFI GGGAC 1 cut(s) 778
BslI CCNNNNNNNGG 4 cut(s) 337, 510, 655, 656
BsmAI GTCTC 3 cut(s) 461, 788, 1022
BsmFI GGGAC 1 cut(s) 778
BsnI GGCC 3 cut(s) 858, 915, 1064
Bsp120I GGGCCC 1 cut(s) 1062
Bsp1286I GDGCHC 3 cut(s) 939, 1066, 1120
Bsp19I CCATGG 2 cut(s) 93, 703
BspACI CCGC 3 cut(s) 194, 762, 1080
BspANI GGCC 3 cut(s) 858, 915, 1064
BspCNI CTCAG 2 cut(s) 301, 511
BspHI TCATGA 1 cut(s) 790
BspLI GGNNCC 4 cut(s) 73, 630, 766, 1064
BspMI ACCTGC 1 cut(s) 1096
BspPI GGATC 7 cut(s) 24, 29, 66, 79, 546, 623, 636
BspQI GCTCTTC 1 cut(s) 1120
BsrDI GCAATG 1 cut(s) 768
BsrI ACTGG 1 cut(s) 726
BssECI CCNNGG 6 cut(s) 93, 488, 504, 703, 978, 1065
BssNI GRCGYC 1 cut(s) 787
BssT1I CCWWGG 4 cut(s) 93, 488, 703, 978
Bst2UI CCWGG 3 cut(s) 505, 860, 1067
Bst4CI ACNGT 3 cut(s) 147, 949, 1017
Bst6I CTCTTC 2 cut(s) 1104, 1120
BstACI GRCGYC 1 cut(s) 787
BstC8I GCNNGC 1 cut(s) 894
BstDEI CTNAG 3 cut(s) 309, 344, 519
BstDSI CCRYGG 2 cut(s) 93, 703
BstF5I GGATG 3 cut(s) 236, 457, 469
BstHHI GCGC 1 cut(s) 754
BstMAI GTCTC 3 cut(s) 461, 788, 1022
BstMWI GCNNNNNNNGC 3 cut(s) 515, 722, 1136
BstNI CCWGG 3 cut(s) 505, 860, 1067
BstNSI RCATGY 1 cut(s) 173
BstSCI CCNGG 3 cut(s) 503, 858, 1065
BstSFI CTRYAG 1 cut(s) 120
BstSLI GKGCMC 1 cut(s) 1066
BstV1I GCAGC 3 cut(s) 295, 760, 1126
BstV2I GAAGAC 1 cut(s) 19
BstX2I RGATCY 5 cut(s) 71, 77, 210, 538, 628
BstXI CCANNNNNNTGG 1 cut(s) 1161
BstYI RGATCY 5 cut(s) 71, 77, 210, 538, 628
BsuRI GGCC 3 cut(s) 858, 915, 1064
BtgI CCRYGG 2 cut(s) 93, 703
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 3 cut(s) 236, 457, 469
BtsI GCAGTG 2 cut(s) 711, 972
BtsIMutI CAGTG 3 cut(s) 405, 711, 972
BveI ACCTGC 1 cut(s) 1096
Cac8I GCNNGC 1 cut(s) 894
CaiI CAGNNNCTG 1 cut(s) 610
CciI TCATGA 1 cut(s) 790
CfoI GCGC 1 cut(s) 754
Cfr13I GGNCC 5 cut(s) 501, 765, 856, 1062, 1063
CseI GACGC 1 cut(s) 776
Csp6I GTAC 1 cut(s) 607
CviAII CATG 7 cut(s) 94, 170, 436, 533, 580, 704, 791
CviQI GTAC 1 cut(s) 607
DdeI CTNAG 3 cut(s) 309, 344, 519
DraI TTTAAA 1 cut(s) 261
EaeI YGGCCR 1 cut(s) 913
Eam1104I CTCTTC 2 cut(s) 1104, 1120
EarI CTCTTC 2 cut(s) 1104, 1120
Ecl136II GAGCTC 1 cut(s) 1118
Eco130I CCWWGG 4 cut(s) 93, 488, 703, 978
Eco24I GRGCYC 2 cut(s) 1066, 1120
Eco32I GATATC 1 cut(s) 885
Eco47I GGWCC 2 cut(s) 501, 765
Eco53kI GAGCTC 1 cut(s) 1118
Eco57I CTGAAG 1 cut(s) 429
EcoICRI GAGCTC 1 cut(s) 1118
EcoO109I RGGNCCY 1 cut(s) 1062
EcoRII CCWGG 3 cut(s) 503, 858, 1065
EcoRV GATATC 1 cut(s) 885
EcoT14I CCWWGG 4 cut(s) 93, 488, 703, 978
EcoT38I GRGCYC 2 cut(s) 1066, 1120
ErhI CCWWGG 4 cut(s) 93, 488, 703, 978
FaeI CATG 7 cut(s) 97, 173, 439, 536, 583, 707, 794
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 1 cut(s) 778
FatI CATG 7 cut(s) 93, 169, 435, 532, 579, 703, 790
FauI CCCGC 1 cut(s) 755
FbaI TGATCA 1 cut(s) 402
FblI GTMKAC 2 cut(s) 576, 969
Fnu4HI GCNGC 4 cut(s) 194, 284, 774, 1140
FokI GGATG 3 cut(s) 243, 464, 476
FriOI GRGCYC 2 cut(s) 1066, 1120
Fsp4HI GCNGC 4 cut(s) 194, 284, 774, 1140
FspBI CTAG 3 cut(s) 66, 477, 1047
FspI TGCGCA 1 cut(s) 753
GlaI GCGC 1 cut(s) 753
GluI GCNGC 4 cut(s) 194, 284, 774, 1140
GsuI CTGGAG 1 cut(s) 1019
HaeIII GGCC 3 cut(s) 858, 915, 1064
HgaI GACGC 1 cut(s) 776
HhaI GCGC 1 cut(s) 754
Hin1I GRCGYC 1 cut(s) 787
Hin1II CATG 7 cut(s) 97, 173, 439, 536, 583, 707, 794
Hin6I GCGC 1 cut(s) 752
HinP1I GCGC 1 cut(s) 752
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HinfI GANTC 4 cut(s) 106, 311, 321, 1095
Hpy166II GTNNAC 3 cut(s) 252, 577, 970
Hpy188I TCNGA 2 cut(s) 21, 316
Hpy188III TCNNGA 8 cut(s) 75, 84, 302, 368, 621, 680, 791, 998
Hpy8I GTNNAC 3 cut(s) 252, 577, 970
HpyAV CCTTC 6 cut(s) 45, 132, 221, 311, 703, 1046
HpyCH4III ACNGT 3 cut(s) 147, 949, 1017
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 4 cut(s) 169, 357, 896, 1091
HpyF10VI GCNNNNNNNGC 3 cut(s) 515, 722, 1136
HpyF3I CTNAG 3 cut(s) 309, 344, 519
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 787
Hsp92II CATG 7 cut(s) 97, 173, 439, 536, 583, 707, 794
HspAI GCGC 1 cut(s) 752
Ksp22I TGATCA 1 cut(s) 402
LguI GCTCTTC 1 cut(s) 1120
LmnI GCTCC 2 cut(s) 340, 473
Lsp1109I GCAGC 3 cut(s) 295, 760, 1126
MaeI CTAG 3 cut(s) 66, 477, 1047
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 127
MboII GAAGA 7 cut(s) 19, 72, 290, 422, 886, 1121, 1137
MflI RGATCY 5 cut(s) 71, 77, 210, 538, 628
MhlI GDGCHC 3 cut(s) 939, 1066, 1120
MlsI TGGCCA 1 cut(s) 915
MluCI AATT 2 cut(s) 831, 1104
MluNI TGGCCA 1 cut(s) 915
MlyI GAGTC 1 cut(s) 320
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 7 cut(s) 118, 157, 165, 514, 576, 777, 951
Mox20I TGGCCA 1 cut(s) 915
MroXI GAANNNNTTC 1 cut(s) 417
MscI TGGCCA 1 cut(s) 915
MseI TTAA 5 cut(s) 216, 260, 546, 902, 1189
Msp20I TGGCCA 1 cut(s) 915
MspA1I CMGCKG 1 cut(s) 1161
MspR9I CCNGG 3 cut(s) 505, 860, 1067
MvaI CCWGG 3 cut(s) 505, 860, 1067
MwoI GCNNNNNNNGC 3 cut(s) 515, 722, 1136
NcoI CCATGG 2 cut(s) 93, 703
NlaIII CATG 7 cut(s) 97, 173, 439, 536, 583, 707, 794
NlaIV GGNNCC 4 cut(s) 73, 630, 766, 1064
NsbI TGCGCA 1 cut(s) 753
NspI RCATGY 1 cut(s) 173
PagI TCATGA 1 cut(s) 790
PciSI GCTCTTC 1 cut(s) 1120
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 3 cut(s) 106, 321, 1095
PflFI GACNNNGTC 2 cut(s) 332, 1073
PflMI CCANNNNNTGG 1 cut(s) 510
PkrI GCNGC 4 cut(s) 195, 285, 775, 1141
PleI GAGTC 1 cut(s) 319
PpsI GAGTC 1 cut(s) 319
Psp124BI GAGCTC 1 cut(s) 1120
Psp6I CCWGG 3 cut(s) 503, 858, 1065
PspGI CCWGG 3 cut(s) 503, 858, 1065
PspN4I GGNNCC 4 cut(s) 73, 630, 766, 1064
PspOMI GGGCCC 1 cut(s) 1062
PspPI GGNCC 5 cut(s) 501, 765, 856, 1062, 1063
PstNI CAGNNNCTG 1 cut(s) 610
PsuI RGATCY 5 cut(s) 71, 77, 210, 538, 628
PsyI GACNNNGTC 2 cut(s) 332, 1073
PvuII CAGCTG 1 cut(s) 1161
RsaI GTAC 1 cut(s) 608
RsaNI GTAC 1 cut(s) 607
SacI GAGCTC 1 cut(s) 1120
SapI GCTCTTC 1 cut(s) 1120
SaqAI TTAA 5 cut(s) 216, 260, 546, 902, 1189
SatI GCNGC 4 cut(s) 194, 284, 774, 1140
Sau96I GGNCC 5 cut(s) 501, 765, 856, 1062, 1063
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 3 cut(s) 505, 860, 1067
SduI GDGCHC 3 cut(s) 939, 1066, 1120
SfcI CTRYAG 1 cut(s) 120
SinI GGWCC 2 cut(s) 501, 765
SmlI CTYRAG 1 cut(s) 1119
SmoI CTYRAG 1 cut(s) 1119
Sse9I AATT 2 cut(s) 831, 1104
SsiI CCGC 3 cut(s) 194, 762, 1080
SspMI CTAG 3 cut(s) 66, 477, 1047
SstI GAGCTC 1 cut(s) 1120
StyD4I CCNGG 3 cut(s) 503, 858, 1065
StyI CCWWGG 4 cut(s) 93, 488, 703, 978
TaaI ACNGT 3 cut(s) 147, 949, 1017
TaiI ACGT 1 cut(s) 48
TaqI TCGA 1 cut(s) 1174
TasI AATT 2 cut(s) 831, 1104
TauI GCSGC 1 cut(s) 196
TfiI GAWTC 3 cut(s) 106, 321, 1095
Tru1I TTAA 5 cut(s) 216, 260, 546, 902, 1189
Tru9I TTAA 5 cut(s) 216, 260, 546, 902, 1189
TscAI CASTG 3 cut(s) 412, 718, 979
TseI GCWGC 3 cut(s) 283, 773, 1139
TspDTI ATGAA 5 cut(s) 27, 255, 652, 685, 887
TspGWI ACGGA 1 cut(s) 1088
TspRI CASTG 3 cut(s) 412, 718, 979
Tth111I GACNNNGTC 2 cut(s) 332, 1073
Van91I CCANNNNNTGG 1 cut(s) 510
VpaK11BI GGWCC 2 cut(s) 501, 765
XceI RCATGY 1 cut(s) 173
XcmI CCANNNNNNNNNTGG 1 cut(s) 91
XmiI GTMKAC 2 cut(s) 576, 969
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 3 cut(s) 66, 477, 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.