Prupe.6G365100_v2.0.a1

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0010828
Physical Location & Seq
Forward (+)
30643868 .. 30648068
4201 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G365100.2

Sequence Viewer

Length: 1182 bp
ATGGCTCTGAGTCTCAATTCCATGATGACCTTCCCATCTCACAACCTCCCTTCTAAGTCCCACAATCTCAGATCTCCAAAAATCTTCATGGCCTCCACTCTTCACTCCTCCCCTAAAGAGACCGAGAATCTGAAGAAGTCTTATTGCCCTCCACGTGAGACACATGTTCAAGTGACTCATTCAATGCCTCCTGAACAGATTGAGATCTTCACATCATTAGAAGGTTGGGCTGAGAATAACATCTTGGTGCACCTAAAGCCTGTTGAGAAGTGCTGGCAACCACAAGATTTTCTTCCACTCCCTGAGTCAGATGGATTCTATGAGCAAGTTAAGGAGCTGAGGGAAAGAGCAAAGGAAATTCCTGATGATTATTTTGTTGTGTTGGTTGGAGATATGATCACCGAAGAAGCCCTACCAACATACCAGACAATGCTTAATACCTTAGATGGAGTTCGGGATGAGACTGGTGCAAGCCTTACTTCCTGGGCAGTATGGACCAGGGCCTGGACTGCCGAAGAGAATAGGCATGGTGACCTTCTTCACAAGTATCTTTATCTCTCGGGAAGAGTAGATATGAGACAAATCGAGAAGACAATTCAGTATCTTATTGGATCAGGAATGGACCCTAAAACAGAAAACAATCCCTACCTTGGGTTCATATATACTTCATTTCAAGAGAGGGCAACATTCGTATCCCATGGGAACACAGCCAGGCAAGCCAAAGAGCACGGGGACCTGAAATTGGCTCAGATATGTGGTACCATTGCCTCAGATGAAAAACGCCATGAAACTGCCTATACAAAAATTGTGGAAAAGCTCTTTGAGATTGATCCAGATGGCACTGTCTTGGCTTTAGCTGACATGATGAAGAAAAAAATATCAATGCCAGCCCACTTGATGTACGATGGTCAAGATGATAATCTTTTTGAACATTTCTCGGCTGTTGCCCAGCGGCTTGAAGTGTATACTGCTAGGGATTATGCTGATATTTTGGAGTTTTTGGTTGGAAGATGGAACATAGAGAAGTTGACGGGTCTTTCTGGCGAGGGACGTAGAGCACAAGATTTCGTCTGTGGACTGCCGCCAAGAATTAGAAGGTTGGAGGAGAGGGCTCAGGGAAGGGCGAAGCAAGCAACCACTGTTCCATTCAGCTGGATTTTTGGTAGAGAAATAAAGGTATAA

Protein Analysis

394

Amino Acids

44.97

Weight (kDa)

5.85

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02630 AT5G16230 AT5G16240
fragaria_vesca FvH4_6g00080
malus_domestica MD00G1017300.v1.1 MD12G1261600.v1.1
prunus_persica Prupe.6G365100_v2.0.a1 Prupe.6G365100_v2.0.a1 Prupe.6G365200_v2.0.a1
pyrus_communis pycom04g21990
rosa_chinensis RchiOBHm_Chr3g0447411
rosa_laevigata RLG00000026001
rosa_multiflora Rmu_ssc0000473.1_g000020
rosa_roxburghii Rroxscaffold_6G00425610
rosa_rugosa Rorug02G0601300
rosa_samantha Rh3AG001400 Rh3BG001100 Rh3CG001000 Rh3DG001300
rosa_wichuraiana Rw3G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 758
AccB1I GGYRCC 1 cut(s) 758
AccB7I CCANNNNNTGG 1 cut(s) 504
AccI GTMKAC 1 cut(s) 965
AciI CCGC 2 cut(s) 952, 1082
AclWI GGATC 2 cut(s) 619, 824
AcsI RAATTY 1 cut(s) 357
AcuI CTGAAG 1 cut(s) 152
AcvI CACGTG 1 cut(s) 155
AfaI GTAC 2 cut(s) 760, 902
AfiI CCNNNNNNNGG 4 cut(s) 504, 650, 651, 742
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 5 cut(s) 170, 183, 674, 929, 959
AjnI CCWGG 4 cut(s) 482, 497, 503, 710
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AluBI AGCT 4 cut(s) 337, 817, 857, 1152
AluI AGCT 4 cut(s) 337, 817, 857, 1152
Alw21I GWGCWC 3 cut(s) 252, 729, 1060
Alw26I GTCTC 5 cut(s) 17, 113, 152, 455, 571
Alw44I GTGCAC 1 cut(s) 248
AlwI GGATC 2 cut(s) 619, 824
AlwNI CAGNNNCTG 1 cut(s) 504
Ama87I CYCGRG 1 cut(s) 559
AoxI GGCC 2 cut(s) 90, 501
ApaLI GTGCAC 1 cut(s) 248
ApoI RAATTY 1 cut(s) 357
Asp718I GGTACC 1 cut(s) 758
AspS9I GGNCC 4 cut(s) 495, 501, 622, 733
AsuHPI GGTGA 2 cut(s) 391, 542
AvaI CYCGRG 1 cut(s) 559
AvaII GGWCC 3 cut(s) 495, 622, 733
BaeGI GKGCMC 1 cut(s) 252
BaeI ACNNNNGTAYC 2 cut(s) 675, 708
BanI GGYRCC 1 cut(s) 758
BanII GRGCYC 1 cut(s) 1114
BarI GAAGNNNNNNTAC 2 cut(s) 396, 428
BbrPI CACGTG 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 596
Bbv12I GWGCWC 3 cut(s) 252, 729, 1060
BbvCI CCTCAGC 1 cut(s) 338
BccI CCATC 6 cut(s) 43, 305, 440, 830, 899, 1005
BciT130I CCWGG 4 cut(s) 484, 499, 505, 712
BciVI GTATCC 1 cut(s) 703
BclI TGATCA 1 cut(s) 396
BcoDI GTCTC 5 cut(s) 17, 113, 152, 455, 571
BfaI CTAG 1 cut(s) 972
BfuI GTATCC 1 cut(s) 703
BglII AGATCT 2 cut(s) 71, 204
BisI GCNGC 2 cut(s) 953, 1082
BlsI GCNGC 2 cut(s) 954, 1083
Bme1390I CCNGG 4 cut(s) 484, 499, 505, 712
Bme18I GGWCC 3 cut(s) 495, 622, 733
BmeT110I CYCGRG 1 cut(s) 559
BmgT120I GGNCC 4 cut(s) 495, 501, 622, 733
BmiI GGNNCC 3 cut(s) 624, 734, 760
BmrFI CCNGG 4 cut(s) 484, 499, 505, 712
BpiI GAAGAC 1 cut(s) 596
Bpu10I CCTNAGC 2 cut(s) 338, 1113
BsaAI YACGTR 1 cut(s) 155
BsaBI GATNNNNATC 2 cut(s) 203, 918
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 4 cut(s) 483, 498, 649, 697
Bsc4I CCNNNNNNNGG 4 cut(s) 504, 650, 651, 742
Bse1I ACTGG 1 cut(s) 469
Bse3DI GCAATG 1 cut(s) 762
Bse8I GATNNNNATC 2 cut(s) 203, 918
BseBI CCWGG 4 cut(s) 484, 499, 505, 712
BseDI CCNNGG 4 cut(s) 483, 498, 649, 697
BseGI GGATG 1 cut(s) 463
BseJI GATNNNNATC 2 cut(s) 203, 918
BseLI CCNNNNNNNGG 4 cut(s) 504, 650, 651, 742
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 7 cut(s) 82, 222, 294, 329, 761, 783, 1127
BseNI ACTGG 1 cut(s) 469
BseRI GAGGAG 2 cut(s) 97, 1118
BseSI GKGCMC 1 cut(s) 252
BseYI CCCAGC 1 cut(s) 948
BshFI GGCC 2 cut(s) 92, 503
BshNI GGYRCC 1 cut(s) 758
BsiHKAI GWGCWC 3 cut(s) 252, 729, 1060
BsiHKCI CYCGRG 1 cut(s) 559
BslFI GGGAC 3 cut(s) 43, 746, 1062
BslI CCNNNNNNNGG 4 cut(s) 504, 650, 651, 742
BsmAI GTCTC 5 cut(s) 17, 113, 152, 455, 571
BsmFI GGGAC 3 cut(s) 43, 746, 1062
BsnI GGCC 2 cut(s) 92, 503
Bso31I GGTCTC 1 cut(s) 113
BsoBI CYCGRG 1 cut(s) 559
Bsp1286I GDGCHC 4 cut(s) 252, 729, 1060, 1114
Bsp143I GATC 5 cut(s) 71, 204, 396, 611, 829
Bsp19I CCATGG 1 cut(s) 697
BspACI CCGC 2 cut(s) 952, 1082
BspANI GGCC 2 cut(s) 92, 503
BspCNI CTCAG 7 cut(s) 81, 223, 295, 330, 760, 782, 1126
BspLI GGNNCC 3 cut(s) 624, 734, 760
BspPI GGATC 2 cut(s) 619, 824
BspT107I GGYRCC 1 cut(s) 758
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 762
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 4 cut(s) 483, 498, 649, 697
BssMI GATC 5 cut(s) 71, 204, 396, 611, 829
BssNAI GTATAC 1 cut(s) 966
BssT1I CCWWGG 2 cut(s) 649, 697
Bst1107I GTATAC 1 cut(s) 966
Bst2UI CCWGG 4 cut(s) 484, 499, 505, 712
Bst4CI ACNGT 2 cut(s) 844, 1141
Bst6I CTCTTC 3 cut(s) 105, 510, 559
BstBAI YACGTR 1 cut(s) 155
BstC8I GCNNGC 5 cut(s) 275, 472, 717, 888, 1131
BstDSI CCRYGG 1 cut(s) 697
BstEII GGTNACC 1 cut(s) 530
BstF5I GGATG 1 cut(s) 463
BstKTI GATC 5 cut(s) 74, 207, 399, 614, 832
BstMAI GTCTC 5 cut(s) 17, 113, 152, 455, 571
BstMBI GATC 5 cut(s) 71, 204, 396, 611, 829
BstMWI GCNNNNNNNGC 4 cut(s) 256, 509, 716, 1130
BstNI CCWGG 4 cut(s) 484, 499, 505, 712
BstNSI RCATGY 1 cut(s) 167
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 4 cut(s) 482, 497, 503, 710
BstSLI GKGCMC 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 596
BstX2I RGATCY 2 cut(s) 71, 204
BstXI CCANNNNNNTGG 1 cut(s) 1152
BstYI RGATCY 2 cut(s) 71, 204
BstZ17I GTATAC 1 cut(s) 966
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 2 cut(s) 92, 503
BtgI CCRYGG 1 cut(s) 697
BtsCI GGATG 1 cut(s) 463
BtsIMutI CAGTG 2 cut(s) 840, 1137
Cac8I GCNNGC 5 cut(s) 275, 472, 717, 888, 1131
CaiI CAGNNNCTG 1 cut(s) 504
Cfr13I GGNCC 4 cut(s) 495, 501, 622, 733
Csp6I GTAC 2 cut(s) 759, 901
CspCI CAANNNNNGTGG 2 cut(s) 789, 824
CviAII CATG 7 cut(s) 22, 88, 164, 527, 698, 785, 862
CviQI GTAC 2 cut(s) 759, 901
DpnI GATC 5 cut(s) 73, 206, 398, 613, 831
DpnII GATC 5 cut(s) 71, 204, 396, 611, 829
Eam1104I CTCTTC 3 cut(s) 105, 510, 559
EarI CTCTTC 3 cut(s) 105, 510, 559
Eco130I CCWWGG 2 cut(s) 649, 697
Eco24I GRGCYC 1 cut(s) 1114
Eco31I GGTCTC 1 cut(s) 113
Eco47I GGWCC 3 cut(s) 495, 622, 733
Eco57I CTGAAG 1 cut(s) 152
Eco72I CACGTG 1 cut(s) 155
Eco88I CYCGRG 1 cut(s) 559
Eco91I GGTNACC 1 cut(s) 530
EcoO109I RGGNCCY 2 cut(s) 501, 733
EcoO65I GGTNACC 1 cut(s) 530
EcoRII CCWGG 4 cut(s) 482, 497, 503, 710
EcoT14I CCWWGG 2 cut(s) 649, 697
EcoT38I GRGCYC 1 cut(s) 1114
ErhI CCWWGG 2 cut(s) 649, 697
FaeI CATG 7 cut(s) 25, 91, 167, 530, 701, 788, 865
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 463, 495
FaqI GGGAC 3 cut(s) 43, 746, 1062
FatI CATG 7 cut(s) 21, 87, 163, 526, 697, 784, 861
FbaI TGATCA 1 cut(s) 396
FblI GTMKAC 1 cut(s) 965
Fnu4HI GCNGC 2 cut(s) 953, 1082
FokI GGATG 1 cut(s) 470
FriOI GRGCYC 1 cut(s) 1114
Fsp4HI GCNGC 2 cut(s) 953, 1082
FspBI CTAG 1 cut(s) 972
GluI GCNGC 2 cut(s) 953, 1082
GsaI CCCAGC 1 cut(s) 952
HaeIII GGCC 2 cut(s) 92, 503
Hin1II CATG 7 cut(s) 25, 91, 167, 530, 701, 788, 865
HincII GTYRAC 1 cut(s) 1029
HindII GTYRAC 1 cut(s) 1029
HinfI GANTC 5 cut(s) 10, 127, 175, 305, 315
HphI GGTGA 2 cut(s) 391, 542
Hpy166II GTNNAC 4 cut(s) 250, 966, 1029, 1076
Hpy188I TCNGA 6 cut(s) 9, 71, 132, 310, 750, 772
Hpy188III TCNNGA 9 cut(s) 191, 362, 455, 561, 586, 615, 674, 833, 911
Hpy8I GTNNAC 4 cut(s) 250, 966, 1029, 1076
HpyAV CCTTC 6 cut(s) 40, 60, 215, 545, 1089, 1113
HpyCH4III ACNGT 2 cut(s) 844, 1141
HpyCH4IV ACGT 2 cut(s) 154, 1051
HpyCH4V TGCA 2 cut(s) 250, 470
HpyF10VI GCNNNNNNNGC 4 cut(s) 256, 509, 716, 1130
HpySE526I ACGT 2 cut(s) 154, 1051
Hsp92II CATG 7 cut(s) 25, 91, 167, 530, 701, 788, 865
KpnI GGTACC 1 cut(s) 762
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 5 cut(s) 71, 204, 396, 611, 829
LmnI GCTCC 1 cut(s) 334
MaeI CTAG 1 cut(s) 972
MaeII ACGT 2 cut(s) 154, 1051
MaeIII GTNAC 2 cut(s) 172, 530
MalI GATC 5 cut(s) 73, 206, 398, 613, 831
MboI GATC 5 cut(s) 71, 204, 396, 611, 829
MflI RGATCY 2 cut(s) 71, 204
MhlI GDGCHC 4 cut(s) 252, 729, 1060, 1114
MluCI AATT 6 cut(s) 16, 357, 594, 740, 804, 1089
MlyI GAGTC 3 cut(s) 19, 169, 314
MmeI TCCRAC 3 cut(s) 367, 985, 1080
MseI TTAA 2 cut(s) 330, 435
MslI CAYNNNNRTG 1 cut(s) 245
MspA1I CMGCKG 2 cut(s) 952, 1152
MspR9I CCNGG 4 cut(s) 484, 499, 505, 712
MvaI CCWGG 4 cut(s) 484, 499, 505, 712
MwoI GCNNNNNNNGC 4 cut(s) 256, 509, 716, 1130
NcoI CCATGG 1 cut(s) 697
NdeII GATC 5 cut(s) 71, 204, 396, 611, 829
NlaIII CATG 7 cut(s) 25, 91, 167, 530, 701, 788, 865
NlaIV GGNNCC 3 cut(s) 624, 734, 760
NmeAIII GCCGAG 1 cut(s) 917
NmuCI GTSAC 2 cut(s) 172, 530
NspI RCATGY 1 cut(s) 167
PciI ACATGT 1 cut(s) 163
PfeI GAWTC 2 cut(s) 127, 315
PflMI CCANNNNNTGG 1 cut(s) 504
PkrI GCNGC 2 cut(s) 954, 1083
PleI GAGTC 3 cut(s) 18, 169, 313
PmaCI CACGTG 1 cut(s) 155
PmlI CACGTG 1 cut(s) 155
PpsI GAGTC 3 cut(s) 18, 169, 313
Ppu21I YACGTR 1 cut(s) 155
PpuMI RGGWCCY 1 cut(s) 733
PscI ACATGT 1 cut(s) 163
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 4 cut(s) 482, 497, 503, 710
PspCI CACGTG 1 cut(s) 155
PspEI GGTNACC 1 cut(s) 530
PspFI CCCAGC 1 cut(s) 948
PspGI CCWGG 4 cut(s) 482, 497, 503, 710
PspN4I GGNNCC 3 cut(s) 624, 734, 760
PspPI GGNCC 4 cut(s) 495, 501, 622, 733
PspPPI RGGWCCY 1 cut(s) 733
PstNI CAGNNNCTG 1 cut(s) 504
PsuI RGATCY 2 cut(s) 71, 204
PvuII CAGCTG 1 cut(s) 1152
RsaI GTAC 2 cut(s) 760, 902
RsaNI GTAC 2 cut(s) 759, 901
RseI CAYNNNNRTG 1 cut(s) 245
SaqAI TTAA 2 cut(s) 330, 435
SatI GCNGC 2 cut(s) 953, 1082
Sau3AI GATC 5 cut(s) 71, 204, 396, 611, 829
Sau96I GGNCC 4 cut(s) 495, 501, 622, 733
SchI GAGTC 3 cut(s) 19, 169, 314
ScrFI CCNGG 4 cut(s) 484, 499, 505, 712
SduI GDGCHC 4 cut(s) 252, 729, 1060, 1114
SinI GGWCC 3 cut(s) 495, 622, 733
SmiMI CAYNNNNRTG 1 cut(s) 245
Sse9I AATT 6 cut(s) 16, 357, 594, 740, 804, 1089
SsiI CCGC 2 cut(s) 952, 1082
SspMI CTAG 1 cut(s) 972
StyD4I CCNGG 4 cut(s) 482, 497, 503, 710
StyI CCWWGG 2 cut(s) 649, 697
TaaI ACNGT 2 cut(s) 844, 1141
TaiI ACGT 2 cut(s) 157, 1054
TaqI TCGA 1 cut(s) 585
TaqII GACCGA 1 cut(s) 137
TasI AATT 6 cut(s) 16, 357, 594, 740, 804, 1089
TauI GCSGC 2 cut(s) 955, 1084
TfiI GAWTC 2 cut(s) 127, 315
Tru1I TTAA 2 cut(s) 330, 435
Tru9I TTAA 2 cut(s) 330, 435
TscAI CASTG 2 cut(s) 847, 1144
TseFI GTSAC 2 cut(s) 172, 530
Tsp45I GTSAC 2 cut(s) 172, 530
TspDTI ATGAA 6 cut(s) 76, 646, 657, 789, 801, 881
TspRI CASTG 2 cut(s) 847, 1144
Van91I CCANNNNNTGG 1 cut(s) 504
VneI GTGCAC 1 cut(s) 248
VpaK11BI GGWCC 3 cut(s) 495, 622, 733
XapI RAATTY 1 cut(s) 357
XceI RCATGY 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 965
XspI CTAG 1 cut(s) 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.