Rmu_ssc0000473.1_g000020

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000473.1
OG0010828
Physical Location & Seq
Reverse (-)
117003 .. 120801
3799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000473.1_g000020.1.cds

Sequence Viewer

Length: 1200 bp
atggctctctgcaggctcaacacctttccatctcacacgctcatcccttgttcttcttcttcttcttcttctaattcccgcaatttcagatctccaaaaatctccatggcctccactcgttattccgtctccagagagactgagaagatgaagaaaactcaattccctccacgtgagtcacacattcaagtgactcattccatgcctcctgaacaccttgagatcttcaagtccttagaggagtgggctgatgataacatcctggtgcacctaaagcctgttgagaaatgttggcaagcacaagattttcttcctgcccctgagtcggagggattctatgagcaagtaaaggagttgagggaaagagcaaaagaaattcctgatgattattttgttgtcttggttggagatatggtcacagaagaagctcttccaacataccagacaatgcttaatactctagatggagttcgggatgagactggtgcaagcctcacctcctgggcaagatggaccagggcatggactgctgaagagaataggcatggagaccttctaaacaagtatctctatctctctggacgagtagatatgaaacaaatcgagaagacaattcagtatcttattggatcaggaatggatcctaaaacggagaacaatccctaccttggtttcatttatacttcatttcaagagagggctaccttcatctcccatgggaacacagccaggcaagcaaaagagcatggggatgtgaaattggctcagatatgtggcaccattgcctcggatgaaaaacgtcatgaaactgcctatacaaagattgtggagaagctctttgagatagacccagatggcactgtattggctttagctgacatgatgaagaaaaaaataacaatgcctgcccacttgatgtatgatggtcaggattataatcttttcgagaatttctcagctgttgcccagcggctggaagtttatacggccaaggattacgctgatattttggagtttctggttggtagatggaaaatagaggagttgactggtctttctggtgagggtcgtagagcacaagatttcgtatgtggattgccaccgagaattagaaggttggaggagagagctcagggaagagccaagaaatcatccagtgttccattcagctggctttttggtagagagataatcgtttaa

Protein Analysis

399

Amino Acids

45.69

Weight (kDa)

6.0

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02630 AT5G16230 AT5G16240
fragaria_vesca FvH4_6g00080
malus_domestica MD00G1017300.v1.1 MD12G1261600.v1.1
prunus_persica Prupe.6G365100_v2.0.a1 Prupe.6G365100_v2.0.a1 Prupe.6G365200_v2.0.a1
pyrus_communis pycom04g21990
rosa_chinensis RchiOBHm_Chr3g0447411
rosa_laevigata RLG00000026001
rosa_multiflora Rmu_ssc0000473.1_g000020
rosa_roxburghii Rroxscaffold_6G00425610
rosa_rugosa Rorug02G0601300
rosa_samantha Rh3AG001400 Rh3BG001100 Rh3CG001000 Rh3DG001300
rosa_wichuraiana Rw3G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 936
AccB1I GGYRCC 1 cut(s) 776
AccB7I CCANNNNNTGG 2 cut(s) 522, 973
AciI CCGC 2 cut(s) 79, 970
AclWI GGATC 3 cut(s) 635, 637, 648
AcoI YGGCCR 1 cut(s) 987
AcsI RAATTY 2 cut(s) 375, 949
AcuI CTGAAG 1 cut(s) 552
AcvI CACGTG 1 cut(s) 173
AfiI CCNNNNNNNGG 4 cut(s) 325, 522, 668, 973
AgsI TTSAA 3 cut(s) 188, 229, 692
AjnI CCWGG 4 cut(s) 261, 500, 515, 728
AluBI AGCT 6 cut(s) 428, 835, 875, 959, 1130, 1170
AluI AGCT 6 cut(s) 428, 835, 875, 959, 1130, 1170
Alw21I GWGCWC 3 cut(s) 270, 1078, 1132
Alw26I GTCTC 4 cut(s) 131, 133, 473, 543
Alw44I GTGCAC 1 cut(s) 266
AlwI GGATC 3 cut(s) 635, 637, 648
AlwNI CAGNNNCTG 1 cut(s) 973
AoxI GGCC 2 cut(s) 108, 987
ApaLI GTGCAC 1 cut(s) 266
ApoI RAATTY 2 cut(s) 375, 949
Asp700I GAANNNNTTC 1 cut(s) 429
AspS9I GGNCC 1 cut(s) 513
AsuHPI GGTGA 2 cut(s) 487, 1073
AvaII GGWCC 1 cut(s) 513
BaeGI GKGCMC 1 cut(s) 270
BamHI GGATCC 1 cut(s) 640
BanI GGYRCC 1 cut(s) 776
BanII GRGCYC 1 cut(s) 1132
BbrPI CACGTG 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 3 cut(s) 270, 1078, 1132
BccI CCATC 6 cut(s) 37, 458, 504, 848, 917, 1023
BceAI ACGGC 1 cut(s) 1002
BciT130I CCWGG 4 cut(s) 263, 502, 517, 730
BcoDI GTCTC 4 cut(s) 131, 133, 473, 543
BfaI CTAG 1 cut(s) 461
BfmI CTRYAG 1 cut(s) 10
BglII AGATCT 2 cut(s) 89, 222
BisI GCNGC 1 cut(s) 971
BlsI GCNGC 1 cut(s) 972
Bme1390I CCNGG 4 cut(s) 263, 502, 517, 730
Bme18I GGWCC 1 cut(s) 513
BmgT120I GGNCC 1 cut(s) 513
BmiI GGNNCC 2 cut(s) 642, 778
BmrFI CCNGG 4 cut(s) 263, 502, 517, 730
BpiI GAAGAC 1 cut(s) 614
BplI GAGNNNNNCTC 2 cut(s) 938, 970
BpmI CTGGAG 1 cut(s) 115
Bpu10I CCTNAGC 1 cut(s) 1131
BpuEI CTTGAG 1 cut(s) 239
BsaAI YACGTR 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 936
BsaI GGTCTC 1 cut(s) 543
BsaJI CCNNGG 7 cut(s) 105, 501, 516, 667, 715, 786, 990
BsaXI ACNNNNNCTCC 2 cut(s) 399, 429
Bsc4I CCNNNNNNNGG 4 cut(s) 325, 522, 668, 973
Bse1I ACTGG 3 cut(s) 487, 1054, 1155
Bse3DI GCAATG 1 cut(s) 780
Bse8I GATNNNNATC 1 cut(s) 936
BseBI CCWGG 4 cut(s) 263, 502, 517, 730
BseDI CCNNGG 7 cut(s) 105, 501, 516, 667, 715, 786, 990
BseGI GGATG 6 cut(s) 42, 258, 481, 757, 796, 1151
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 4 cut(s) 325, 522, 668, 973
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 5 cut(s) 132, 312, 779, 969, 1145
BseNI ACTGG 3 cut(s) 487, 1054, 1155
BseRI GAGGAG 3 cut(s) 254, 1055, 1136
BseSI GKGCMC 1 cut(s) 270
BseYI CCCAGC 1 cut(s) 966
BshFI GGCC 2 cut(s) 110, 989
BshNI GGYRCC 1 cut(s) 776
BsiHKAI GWGCWC 3 cut(s) 270, 1078, 1132
BslI CCNNNNNNNGG 4 cut(s) 325, 522, 668, 973
BsmAI GTCTC 4 cut(s) 131, 133, 473, 543
BsmBI CGTCTC 1 cut(s) 133
BsnI GGCC 2 cut(s) 110, 989
Bso31I GGTCTC 1 cut(s) 543
Bsp1286I GDGCHC 3 cut(s) 270, 1078, 1132
Bsp143I GATC 4 cut(s) 89, 222, 629, 640
Bsp19I CCATGG 2 cut(s) 105, 715
BspACI CCGC 2 cut(s) 79, 970
BspANI GGCC 2 cut(s) 110, 989
BspCNI CTCAG 5 cut(s) 133, 313, 778, 968, 1144
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 2 cut(s) 642, 778
BspMAI CTGCAG 1 cut(s) 14
BspPI GGATC 3 cut(s) 635, 637, 648
BspQI GCTCTTC 2 cut(s) 435, 1132
BspT107I GGYRCC 1 cut(s) 776
BspTNI GGTCTC 1 cut(s) 543
BsrDI GCAATG 1 cut(s) 780
BsrI ACTGG 3 cut(s) 487, 1054, 1155
BssECI CCNNGG 7 cut(s) 105, 501, 516, 667, 715, 786, 990
BssMI GATC 4 cut(s) 89, 222, 629, 640
BssT1I CCWWGG 4 cut(s) 105, 667, 715, 990
Bst2UI CCWGG 4 cut(s) 263, 502, 517, 730
Bst4CI ACNGT 1 cut(s) 862
Bst6I CTCTTC 3 cut(s) 435, 528, 1132
BstAPI GCANNNNNTGC 1 cut(s) 527
BstBAI YACGTR 1 cut(s) 173
BstC8I GCNNGC 6 cut(s) 14, 297, 490, 735, 906, 1172
BstDEI CTNAG 6 cut(s) 141, 235, 321, 765, 955, 1131
BstDSI CCRYGG 2 cut(s) 105, 715
BstF5I GGATG 6 cut(s) 42, 258, 481, 757, 796, 1151
BstKTI GATC 4 cut(s) 92, 225, 632, 643
BstMAI GTCTC 4 cut(s) 131, 133, 473, 543
BstMBI GATC 4 cut(s) 89, 222, 629, 640
BstMWI GCNNNNNNNGC 3 cut(s) 274, 527, 734
BstNI CCWGG 4 cut(s) 263, 502, 517, 730
BstSCI CCNGG 4 cut(s) 261, 500, 515, 728
BstSFI CTRYAG 1 cut(s) 10
BstSLI GKGCMC 1 cut(s) 270
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 3 cut(s) 89, 222, 640
BstXI CCANNNNNNTGG 1 cut(s) 1170
BstYI RGATCY 3 cut(s) 89, 222, 640
BsuRI GGCC 2 cut(s) 110, 989
BtgI CCRYGG 2 cut(s) 105, 715
BtsCI GGATG 6 cut(s) 42, 258, 481, 757, 796, 1151
BtsIMutI CAGTG 2 cut(s) 858, 1162
Cac8I GCNNGC 6 cut(s) 14, 297, 490, 735, 906, 1172
CaiI CAGNNNCTG 1 cut(s) 973
CciI TCATGA 1 cut(s) 802
Cfr13I GGNCC 1 cut(s) 513
CspCI CAANNNNNGTGG 2 cut(s) 807, 842
CviAII CATG 8 cut(s) 106, 202, 522, 545, 716, 746, 803, 880
DdeI CTNAG 6 cut(s) 141, 235, 321, 765, 955, 1131
DpnI GATC 4 cut(s) 91, 224, 631, 642
DpnII GATC 4 cut(s) 89, 222, 629, 640
EaeI YGGCCR 1 cut(s) 987
Eam1104I CTCTTC 3 cut(s) 435, 528, 1132
EarI CTCTTC 3 cut(s) 435, 528, 1132
Ecl136II GAGCTC 1 cut(s) 1130
Eco130I CCWWGG 4 cut(s) 105, 667, 715, 990
Eco24I GRGCYC 1 cut(s) 1132
Eco31I GGTCTC 1 cut(s) 543
Eco47I GGWCC 1 cut(s) 513
Eco53kI GAGCTC 1 cut(s) 1130
Eco57I CTGAAG 1 cut(s) 552
Eco72I CACGTG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 1130
EcoRII CCWGG 4 cut(s) 261, 500, 515, 728
EcoT14I CCWWGG 4 cut(s) 105, 667, 715, 990
EcoT38I GRGCYC 1 cut(s) 1132
ErhI CCWWGG 4 cut(s) 105, 667, 715, 990
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 8 cut(s) 109, 205, 525, 548, 719, 749, 806, 883
FalI AAGNNNNNCTT 4 cut(s) 294, 326, 414, 446
FatI CATG 8 cut(s) 105, 201, 521, 544, 715, 745, 802, 879
FauI CCCGC 1 cut(s) 86
Fnu4HI GCNGC 1 cut(s) 971
FokI GGATG 6 cut(s) 29, 245, 488, 764, 803, 1138
FriOI GRGCYC 1 cut(s) 1132
Fsp4HI GCNGC 1 cut(s) 971
FspBI CTAG 1 cut(s) 461
GluI GCNGC 1 cut(s) 971
GsaI CCCAGC 1 cut(s) 970
GsuI CTGGAG 1 cut(s) 115
HaeIII GGCC 2 cut(s) 110, 989
Hin1II CATG 8 cut(s) 109, 205, 525, 548, 719, 749, 806, 883
HincII GTYRAC 1 cut(s) 1047
HindII GTYRAC 1 cut(s) 1047
HinfI GANTC 4 cut(s) 176, 193, 323, 333
HphI GGTGA 2 cut(s) 487, 1073
Hpy166II GTNNAC 2 cut(s) 268, 1047
Hpy188I TCNGA 4 cut(s) 89, 328, 768, 790
Hpy8I GTNNAC 2 cut(s) 268, 1047
HpyAV CCTTC 3 cut(s) 563, 715, 1107
HpyCH4III ACNGT 1 cut(s) 862
HpyCH4IV ACGT 2 cut(s) 172, 799
HpyCH4V TGCA 3 cut(s) 12, 268, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 274, 527, 734
HpyF3I CTNAG 6 cut(s) 141, 235, 321, 765, 955, 1131
HpySE526I ACGT 2 cut(s) 172, 799
Hsp92II CATG 8 cut(s) 109, 205, 525, 548, 719, 749, 806, 883
Kzo9I GATC 4 cut(s) 89, 222, 629, 640
LguI GCTCTTC 2 cut(s) 435, 1132
MaeI CTAG 1 cut(s) 461
MaeII ACGT 2 cut(s) 172, 799
MaeIII GTNAC 3 cut(s) 177, 190, 415
MalI GATC 4 cut(s) 91, 224, 631, 642
MboI GATC 4 cut(s) 89, 222, 629, 640
MflI RGATCY 3 cut(s) 89, 222, 640
MhlI GDGCHC 3 cut(s) 270, 1078, 1132
MluCI AATT 8 cut(s) 73, 82, 161, 375, 612, 758, 949, 1107
MlyI GAGTC 3 cut(s) 185, 187, 332
MmeI TCCRAC 4 cut(s) 306, 385, 458, 1098
MroXI GAANNNNTTC 1 cut(s) 429
MseI TTAA 2 cut(s) 453, 1198
MslI CAYNNNNRTG 3 cut(s) 188, 263, 750
MspA1I CMGCKG 3 cut(s) 959, 970, 1170
MspR9I CCNGG 4 cut(s) 263, 502, 517, 730
MvaI CCWGG 4 cut(s) 263, 502, 517, 730
MwoI GCNNNNNNNGC 3 cut(s) 274, 527, 734
NcoI CCATGG 2 cut(s) 105, 715
NdeII GATC 4 cut(s) 89, 222, 629, 640
NlaIII CATG 8 cut(s) 109, 205, 525, 548, 719, 749, 806, 883
NlaIV GGNNCC 2 cut(s) 642, 778
NmuCI GTSAC 3 cut(s) 177, 190, 415
PagI TCATGA 1 cut(s) 802
PciSI GCTCTTC 2 cut(s) 435, 1132
PdmI GAANNNNTTC 1 cut(s) 429
PfeI GAWTC 1 cut(s) 333
PflMI CCANNNNNTGG 2 cut(s) 522, 973
PkrI GCNGC 1 cut(s) 972
PleI GAGTC 3 cut(s) 184, 187, 331
PmaCI CACGTG 1 cut(s) 173
PmlI CACGTG 1 cut(s) 173
PpsI GAGTC 3 cut(s) 184, 187, 331
Ppu21I YACGTR 1 cut(s) 173
PsiI TTATAA 1 cut(s) 936
Psp124BI GAGCTC 1 cut(s) 1132
Psp6I CCWGG 4 cut(s) 261, 500, 515, 728
PspCI CACGTG 1 cut(s) 173
PspFI CCCAGC 1 cut(s) 966
PspGI CCWGG 4 cut(s) 261, 500, 515, 728
PspN4I GGNNCC 2 cut(s) 642, 778
PspPI GGNCC 1 cut(s) 513
PsrI GAACNNNNNNTAC 2 cut(s) 647, 679
PstI CTGCAG 1 cut(s) 14
PstNI CAGNNNCTG 1 cut(s) 973
PsuI RGATCY 3 cut(s) 89, 222, 640
PvuII CAGCTG 2 cut(s) 959, 1170
RseI CAYNNNNRTG 3 cut(s) 188, 263, 750
SacI GAGCTC 1 cut(s) 1132
SapI GCTCTTC 2 cut(s) 435, 1132
SaqAI TTAA 2 cut(s) 453, 1198
SatI GCNGC 1 cut(s) 971
Sau3AI GATC 4 cut(s) 89, 222, 629, 640
Sau96I GGNCC 1 cut(s) 513
SchI GAGTC 3 cut(s) 185, 187, 332
ScrFI CCNGG 4 cut(s) 263, 502, 517, 730
SduI GDGCHC 3 cut(s) 270, 1078, 1132
SfcI CTRYAG 1 cut(s) 10
SinI GGWCC 1 cut(s) 513
SmiMI CAYNNNNRTG 3 cut(s) 188, 263, 750
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 8 cut(s) 73, 82, 161, 375, 612, 758, 949, 1107
SsiI CCGC 2 cut(s) 79, 970
SspMI CTAG 1 cut(s) 461
SstI GAGCTC 1 cut(s) 1132
StyD4I CCNGG 4 cut(s) 261, 500, 515, 728
StyI CCWWGG 4 cut(s) 105, 667, 715, 990
TaaI ACNGT 1 cut(s) 862
TaiI ACGT 2 cut(s) 175, 802
TaqI TCGA 2 cut(s) 603, 945
TasI AATT 8 cut(s) 73, 82, 161, 375, 612, 758, 949, 1107
TauI GCSGC 1 cut(s) 973
TfiI GAWTC 1 cut(s) 333
Tru1I TTAA 2 cut(s) 453, 1198
Tru9I TTAA 2 cut(s) 453, 1198
TscAI CASTG 2 cut(s) 865, 1162
TseFI GTSAC 3 cut(s) 177, 190, 415
Tsp45I GTSAC 3 cut(s) 177, 190, 415
TspDTI ATGAA 8 cut(s) 164, 608, 664, 675, 697, 807, 819, 899
TspGWI ACGGA 2 cut(s) 115, 665
TspRI CASTG 2 cut(s) 865, 1162
Van91I CCANNNNNTGG 2 cut(s) 522, 973
VneI GTGCAC 1 cut(s) 266
VpaK11BI GGWCC 1 cut(s) 513
XapI RAATTY 2 cut(s) 375, 949
XbaI TCTAGA 1 cut(s) 460
XmnI GAANNNNTTC 1 cut(s) 429
XspI CTAG 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.