pycom04g21990

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010828
Physical Location & Seq
Forward (+)
22946343 .. 22949927
3585 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g21990.1

Sequence Viewer

Length: 1182 bp
ATGGCTCTGAGTCTGAATTCGATGCTGACCTTCCCGTCTCACAACCTCACTGCCAAGTCCCACAATCTCAAATCTCCCAAAATCTTCATGGCCTCCACTCTTCACTCCTCCACCAAAGAGACTGAGAATCTGAAGAAGTCTTTTGGACCTCCACGTGAGTTACATGTTCAGGTGACTCATTCAATGCCTCCTCAACAGATTGAGATCTTCAAGTCATTAGAGGGTTGGGCTGAGAATAACATCTTGGTGCACCTAAAGCCTGTTGAGAAATGTTGGCAACCACAAGATTTTCTTCCGATCCCTGAGTCAGATGGATTCGAGGAGCAAGTTAAGGAGTTGAGGGAAAGAGCAAGGGAAATTCCTGATGATTACTTTGTTGTGTTGGTTGGAGATATGATCACAGAAGAAGCCCTTCCAACGTACCAGACAATGCTTAATACCCTAGACGGAGTTCGGGATGAGACTGGTGCAAGCCTTACTTCCTGGGCTATTTGGACCAGGGCGTGGACTGCTGAAGAGAATAGGCATGGTGATCTTCTTAACAAGTATCTTTATCTCTCTGGACGAGTAGATATGAGACAAATCGAGAAGACAATTCAGTACCTTATTGGATCTGGAATGGATCCTAAAACAGAGAACAATCCCTACCTCGGGTTCATTTACACTTCATTCCAAGAGAGGGCAACATTCATCTCCCATGGGAACACAGCCAGGCAAGCCAAAGAGCATGGGGACTTGAAATTGGCTCAGATATGTGGCACCATTGCCTCGGATGAAAAGCGTCATGAAACTGCGTATACCAAGATTGTGGAAAAGCTGTTTGAGATTGATCCAGATGGCACTGTGTTGGCCTTAGCTGACATGATGAAGAAAAAGATATCAATGCCTGCCCACTTGATGTACGATGGTCAGGATGATAATCTGTTCGATCATTTCTCAGCTGTTGCCCAGAGGCTTGAGGTGTATACTGCCAGGGATTATGCTGATATATTGGAGTTTTGGGTTGGAAGATGGAGCGTGGAGAAGTTGACTGGTCTTTCTGGTGAGGGACGCAAGGCACAAGATTTTGTGTGTAAATTGCCACCGAGAATCAGGAGGTTGGAGGAAAGAGCACAGGGAAGGGCCAAGGCTTCATCCCTTGTTCCATTCAGCTGGATTTTTGGTAGAGAAGTGAAGGTTTAA

Protein Analysis

394

Amino Acids

44.9

Weight (kDa)

5.99

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 66 - 387 1.4e-159 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02630 AT5G16230 AT5G16240
fragaria_vesca FvH4_6g00080
malus_domestica MD00G1017300.v1.1 MD12G1261600.v1.1
prunus_persica Prupe.6G365100_v2.0.a1 Prupe.6G365100_v2.0.a1 Prupe.6G365200_v2.0.a1
pyrus_communis pycom04g21990
rosa_chinensis RchiOBHm_Chr3g0447411
rosa_laevigata RLG00000026001
rosa_multiflora Rmu_ssc0000473.1_g000020
rosa_roxburghii Rroxscaffold_6G00425610
rosa_rugosa Rorug02G0601300
rosa_samantha Rh3AG001400 Rh3BG001100 Rh3CG001000 Rh3DG001300
rosa_wichuraiana Rw3G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 34
AccB1I GGYRCC 1 cut(s) 758
AccB7I CCANNNNNTGG 1 cut(s) 504
AccI GTMKAC 2 cut(s) 797, 965
AclWI GGATC 5 cut(s) 292, 617, 619, 630, 824
AcsI RAATTY 2 cut(s) 16, 357
AcuI CTGAAG 2 cut(s) 152, 534
AcvI CACGTG 1 cut(s) 155
AfaI GTAC 3 cut(s) 422, 602, 902
AfiI CCNNNNNNNGG 4 cut(s) 504, 650, 651, 679
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 3 cut(s) 183, 211, 739
AjnI CCWGG 4 cut(s) 482, 497, 710, 971
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AluBI AGCT 4 cut(s) 817, 857, 941, 1152
AluI AGCT 4 cut(s) 817, 857, 941, 1152
Alw21I GWGCWC 2 cut(s) 252, 1114
Alw26I GTCTC 4 cut(s) 42, 113, 455, 571
Alw44I GTGCAC 1 cut(s) 248
AlwI GGATC 5 cut(s) 292, 617, 619, 630, 824
Ama87I CYCGRG 1 cut(s) 650
AoxI GGCC 3 cut(s) 90, 849, 1122
ApaLI GTGCAC 1 cut(s) 248
ApoI RAATTY 2 cut(s) 16, 357
Asp700I GAANNNNTTC 1 cut(s) 411
AspS9I GGNCC 3 cut(s) 146, 495, 1122
AsuHPI GGTGA 3 cut(s) 184, 542, 1055
AvaI CYCGRG 1 cut(s) 650
AvaII GGWCC 2 cut(s) 146, 495
BaeGI GKGCMC 1 cut(s) 252
BamHI GGATCC 1 cut(s) 622
BanI GGYRCC 1 cut(s) 758
BbrPI CACGTG 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 596
Bbv12I GWGCWC 2 cut(s) 252, 1114
BccI CCATC 4 cut(s) 305, 830, 899, 1005
BciT130I CCWGG 4 cut(s) 484, 499, 712, 973
BclI TGATCA 1 cut(s) 396
BcoDI GTCTC 4 cut(s) 42, 113, 455, 571
BfaI CTAG 1 cut(s) 443
BglII AGATCT 1 cut(s) 204
Bme1390I CCNGG 4 cut(s) 484, 499, 712, 973
Bme18I GGWCC 2 cut(s) 146, 495
BmeT110I CYCGRG 1 cut(s) 650
BmgT120I GGNCC 3 cut(s) 146, 495, 1122
BmiI GGNNCC 2 cut(s) 624, 760
BmrFI CCNGG 4 cut(s) 484, 499, 712, 973
BmsI GCATC 1 cut(s) 12
BpiI GAAGAC 1 cut(s) 596
Bpu10I CCTNAGC 1 cut(s) 853
BpuEI CTTGAG 1 cut(s) 977
BsaAI YACGTR 1 cut(s) 155
BsaBI GATNNNNATC 2 cut(s) 203, 918
BsaJI CCNNGG 7 cut(s) 483, 498, 649, 697, 768, 972, 1125
Bsc4I CCNNNNNNNGG 4 cut(s) 504, 650, 651, 679
Bse1I ACTGG 2 cut(s) 469, 1036
Bse3DI GCAATG 1 cut(s) 762
Bse8I GATNNNNATC 2 cut(s) 203, 918
BseBI CCWGG 4 cut(s) 484, 499, 712, 973
BseDI CCNNGG 7 cut(s) 483, 498, 649, 697, 768, 972, 1125
BseGI GGATG 4 cut(s) 463, 778, 919, 1133
BseJI GATNNNNATC 2 cut(s) 203, 918
BseLI CCNNNNNNNGG 4 cut(s) 504, 650, 651, 679
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 5 cut(s) 114, 222, 294, 761, 951
BseNI ACTGG 2 cut(s) 469, 1036
BseRI GAGGAG 3 cut(s) 97, 180, 335
BseSI GKGCMC 1 cut(s) 252
BshFI GGCC 3 cut(s) 92, 851, 1124
BshNI GGYRCC 1 cut(s) 758
BsiHKAI GWGCWC 2 cut(s) 252, 1114
BsiHKCI CYCGRG 1 cut(s) 650
BslFI GGGAC 3 cut(s) 43, 746, 1062
BslI CCNNNNNNNGG 4 cut(s) 504, 650, 651, 679
BsmAI GTCTC 4 cut(s) 42, 113, 455, 571
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 3 cut(s) 43, 746, 1062
BsnI GGCC 3 cut(s) 92, 851, 1124
BsoBI CYCGRG 1 cut(s) 650
Bsp1286I GDGCHC 2 cut(s) 252, 1114
Bsp143I GATC 8 cut(s) 204, 297, 396, 532, 611, 622, 829, 928
Bsp19I CCATGG 1 cut(s) 697
BspANI GGCC 3 cut(s) 92, 851, 1124
BspCNI CTCAG 5 cut(s) 115, 223, 295, 760, 950
BspHI TCATGA 1 cut(s) 784
BspLI GGNNCC 2 cut(s) 624, 760
BspPI GGATC 5 cut(s) 292, 617, 619, 630, 824
BspT107I GGYRCC 1 cut(s) 758
BsrDI GCAATG 1 cut(s) 762
BsrI ACTGG 2 cut(s) 469, 1036
BssECI CCNNGG 7 cut(s) 483, 498, 649, 697, 768, 972, 1125
BssMI GATC 8 cut(s) 204, 297, 396, 532, 611, 622, 829, 928
BssNAI GTATAC 2 cut(s) 798, 966
BssT1I CCWWGG 2 cut(s) 697, 1125
Bst1107I GTATAC 2 cut(s) 798, 966
Bst2UI CCWGG 4 cut(s) 484, 499, 712, 973
Bst4CI ACNGT 1 cut(s) 844
Bst6I CTCTTC 2 cut(s) 105, 510
BstBAI YACGTR 1 cut(s) 155
BstC8I GCNNGC 3 cut(s) 472, 717, 888
BstDEI CTNAG 7 cut(s) 8, 123, 231, 303, 747, 853, 937
BstDSI CCRYGG 1 cut(s) 697
BstF5I GGATG 4 cut(s) 463, 778, 919, 1133
BstKTI GATC 8 cut(s) 207, 300, 399, 535, 614, 625, 832, 931
BstMAI GTCTC 4 cut(s) 42, 113, 455, 571
BstMBI GATC 8 cut(s) 204, 297, 396, 532, 611, 622, 829, 928
BstMWI GCNNNNNNNGC 3 cut(s) 256, 509, 716
BstNI CCWGG 4 cut(s) 484, 499, 712, 973
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 4 cut(s) 482, 497, 710, 971
BstSLI GKGCMC 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 596
BstX2I RGATCY 3 cut(s) 204, 611, 622
BstXI CCANNNNNNTGG 2 cut(s) 808, 1152
BstYI RGATCY 3 cut(s) 204, 611, 622
BstZ17I GTATAC 2 cut(s) 798, 966
BsuRI GGCC 3 cut(s) 92, 851, 1124
BtgI CCRYGG 1 cut(s) 697
BtsCI GGATG 4 cut(s) 463, 778, 919, 1133
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 48, 840
Cac8I GCNNGC 3 cut(s) 472, 717, 888
CciI TCATGA 1 cut(s) 784
Cfr13I GGNCC 3 cut(s) 146, 495, 1122
CseI GACGC 2 cut(s) 770, 1059
Csp6I GTAC 3 cut(s) 421, 601, 901
CviAII CATG 7 cut(s) 88, 164, 527, 698, 728, 785, 862
CviQI GTAC 3 cut(s) 421, 601, 901
DdeI CTNAG 7 cut(s) 8, 123, 231, 303, 747, 853, 937
DpnI GATC 8 cut(s) 206, 299, 398, 534, 613, 624, 831, 930
DpnII GATC 8 cut(s) 204, 297, 396, 532, 611, 622, 829, 928
DrdI GACNNNNNNGTC 1 cut(s) 34
DseDI GACNNNNNNGTC 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 105, 510
EarI CTCTTC 2 cut(s) 105, 510
Eco130I CCWWGG 2 cut(s) 697, 1125
Eco32I GATATC 1 cut(s) 879
Eco47I GGWCC 2 cut(s) 146, 495
Eco57I CTGAAG 2 cut(s) 152, 534
Eco72I CACGTG 1 cut(s) 155
Eco88I CYCGRG 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 4 cut(s) 482, 497, 710, 971
EcoRV GATATC 1 cut(s) 879
EcoT14I CCWWGG 2 cut(s) 697, 1125
ErhI CCWWGG 2 cut(s) 697, 1125
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 7 cut(s) 91, 167, 530, 701, 731, 788, 865
FalI AAGNNNNNCTT 6 cut(s) 276, 308, 396, 428, 463, 495
FaqI GGGAC 3 cut(s) 43, 746, 1062
FatI CATG 7 cut(s) 87, 163, 526, 697, 727, 784, 861
FbaI TGATCA 1 cut(s) 396
FblI GTMKAC 2 cut(s) 797, 965
FokI GGATG 4 cut(s) 470, 785, 926, 1120
FspBI CTAG 1 cut(s) 443
HaeIII GGCC 3 cut(s) 92, 851, 1124
HgaI GACGC 2 cut(s) 770, 1059
Hin1II CATG 7 cut(s) 91, 167, 530, 701, 731, 788, 865
HincII GTYRAC 1 cut(s) 1029
HindII GTYRAC 1 cut(s) 1029
HinfI GANTC 6 cut(s) 10, 127, 175, 305, 315, 1089
HphI GGTGA 3 cut(s) 184, 542, 1055
Hpy166II GTNNAC 5 cut(s) 250, 507, 798, 966, 1029
Hpy188I TCNGA 7 cut(s) 9, 15, 132, 297, 310, 750, 772
Hpy188III TCNNGA 9 cut(s) 362, 455, 561, 586, 615, 785, 833, 911, 1093
Hpy8I GTNNAC 5 cut(s) 250, 507, 798, 966, 1029
HpyAV CCTTC 4 cut(s) 40, 422, 1113, 1168
HpyCH4III ACNGT 1 cut(s) 844
HpyCH4IV ACGT 2 cut(s) 154, 419
HpyCH4V TGCA 2 cut(s) 250, 470
HpyF10VI GCNNNNNNNGC 3 cut(s) 256, 509, 716
HpyF3I CTNAG 7 cut(s) 8, 123, 231, 303, 747, 853, 937
HpySE526I ACGT 2 cut(s) 154, 419
Hsp92II CATG 7 cut(s) 91, 167, 530, 701, 731, 788, 865
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 8 cut(s) 204, 297, 396, 532, 611, 622, 829, 928
LmnI GCTCC 2 cut(s) 322, 1014
LweI GCATC 1 cut(s) 12
MaeI CTAG 1 cut(s) 443
MaeII ACGT 2 cut(s) 154, 419
MaeIII GTNAC 2 cut(s) 159, 172
MalI GATC 8 cut(s) 206, 299, 398, 534, 613, 624, 831, 930
MboI GATC 8 cut(s) 204, 297, 396, 532, 611, 622, 829, 928
MflI RGATCY 3 cut(s) 204, 611, 622
MhlI GDGCHC 2 cut(s) 252, 1114
MluCI AATT 5 cut(s) 16, 357, 594, 740, 1076
MlyI GAGTC 3 cut(s) 19, 169, 314
MmeI TCCRAC 4 cut(s) 367, 440, 985, 1080
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 4 cut(s) 330, 435, 540, 1180
MslI CAYNNNNRTG 1 cut(s) 245
MspA1I CMGCKG 2 cut(s) 941, 1152
MspR9I CCNGG 4 cut(s) 484, 499, 712, 973
MvaI CCWGG 4 cut(s) 484, 499, 712, 973
MwoI GCNNNNNNNGC 3 cut(s) 256, 509, 716
NcoI CCATGG 1 cut(s) 697
NdeII GATC 8 cut(s) 204, 297, 396, 532, 611, 622, 829, 928
NlaIII CATG 7 cut(s) 91, 167, 530, 701, 731, 788, 865
NlaIV GGNNCC 2 cut(s) 624, 760
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 1 cut(s) 167
PagI TCATGA 1 cut(s) 784
PciI ACATGT 1 cut(s) 163
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 3 cut(s) 127, 315, 1089
PflMI CCANNNNNTGG 1 cut(s) 504
PleI GAGTC 3 cut(s) 18, 169, 313
PmaCI CACGTG 1 cut(s) 155
PmlI CACGTG 1 cut(s) 155
PpsI GAGTC 3 cut(s) 18, 169, 313
Ppu21I YACGTR 1 cut(s) 155
PscI ACATGT 1 cut(s) 163
Psp6I CCWGG 4 cut(s) 482, 497, 710, 971
PspCI CACGTG 1 cut(s) 155
PspGI CCWGG 4 cut(s) 482, 497, 710, 971
PspN4I GGNNCC 2 cut(s) 624, 760
PspPI GGNCC 3 cut(s) 146, 495, 1122
PsrI GAACNNNNNNTAC 2 cut(s) 629, 661
PsuI RGATCY 3 cut(s) 204, 611, 622
PvuII CAGCTG 2 cut(s) 941, 1152
RsaI GTAC 3 cut(s) 422, 602, 902
RsaNI GTAC 3 cut(s) 421, 601, 901
RseI CAYNNNNRTG 1 cut(s) 245
SaqAI TTAA 4 cut(s) 330, 435, 540, 1180
Sau3AI GATC 8 cut(s) 204, 297, 396, 532, 611, 622, 829, 928
Sau96I GGNCC 3 cut(s) 146, 495, 1122
SchI GAGTC 3 cut(s) 19, 169, 314
ScrFI CCNGG 4 cut(s) 484, 499, 712, 973
SduI GDGCHC 2 cut(s) 252, 1114
SfaNI GCATC 1 cut(s) 12
SinI GGWCC 2 cut(s) 146, 495
SmiMI CAYNNNNRTG 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
Sse9I AATT 5 cut(s) 16, 357, 594, 740, 1076
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 4 cut(s) 482, 497, 710, 971
StyI CCWWGG 2 cut(s) 697, 1125
TaaI ACNGT 1 cut(s) 844
TaiI ACGT 2 cut(s) 157, 422
TaqI TCGA 4 cut(s) 20, 318, 585, 927
TasI AATT 5 cut(s) 16, 357, 594, 740, 1076
TfiI GAWTC 3 cut(s) 127, 315, 1089
Tru1I TTAA 4 cut(s) 330, 435, 540, 1180
Tru9I TTAA 4 cut(s) 330, 435, 540, 1180
TscAI CASTG 2 cut(s) 55, 847
TseFI GTSAC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 8 cut(s) 76, 646, 657, 679, 789, 801, 881, 1122
TspGWI ACGGA 1 cut(s) 462
TspRI CASTG 2 cut(s) 55, 847
Van91I CCANNNNNTGG 1 cut(s) 504
VneI GTGCAC 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 146, 495
XapI RAATTY 2 cut(s) 16, 357
XceI RCATGY 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmiI GTMKAC 2 cut(s) 797, 965
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.