FvH4_6g00080

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010828
Physical Location & Seq
Reverse (-)
69860 .. 73548
3689 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g00080.t1

Sequence Viewer

Length: 1197 bp
ATGGCTCTCAGACTGAATTCCACGGTGAACACCTTCCCATCTCACCAGCTCATGCCTTCTTCTTCCAAGTCCCGCAGTTTCAGATCTCCCAGAATCTTCATGGCTTCCACTCTTCATTCCGACTCCTCCAAGGAGACTGAGAAGATGAAGAAAACTTATTGCCCTCCACGTGAGTCACATGTTCAAGTGACTCATTCCATGTCTCCTGAACACCTTGAGATCTTCTCATCCTTAGAGGAGTGGGCTGACGATAACATTTTGGTGCACCTAAAGCCTGTCGAGAAATGTTGGCAAGCACAAGATTTTCTTCCTGCCCCTGAGTCAGAGGGATTCTATGAGCAAGTAAATGAGTTGAGGGAAAGAGCAAAAGAAATTCCTGATGATTATTTTGTCGTCTTGGTTGGAGATATGATCACAGAAGAAGCTCTTCCAACATACCAGACAATGCTTAATACTCTAGATGGAGTTCGGGATGAGACTGGTGCAAGCCTCACCTCCTGGGCAAGATGGACTAGGGCATGGACTGCTGAAGAGAACAGGCATGGAGACCTTCTAAACAAGTATCTTTATCTCTCTGGACGAGTAGATATGAAACAAATTGAGAAGACAATTCAGTATCTTATAGGATCAGGAATGGATCCTAAAACGGAGAACAATCCCTACCTTGGTTTCATTTATACTTCATTTCAAGAAAGGGCCACCTTCATCTCCCATGGGAACACAGCCAGGCACGCTAAAGAGCATGGGGACCTGAAATTGGCTCAGATATGTGGCACCATTGCCTCGGATGAAAAACGTCATGAAACTGCCTATACAAAGATTGTGGAGAAGCTCTTTGAGATAGACCCGAATGGCACTGTCTTAGCTTTAGCTGACATGATGAAGAAAAAAATATCAATGCCTGCACACCTGATGTATGATGGTCAGGACGATAATCTTTTCGAGCACTTCTCAGCTGTTGCCCAGCGGCTGGAAGTATATACAGCCAAGGATTATGCTGATATTTTGGAGTTTCTGGTTGGTAGATGGAAAGTAGAGGAGTTGTCTGGTCTTTCTGGTGAGGGTCGTAGAGCTCAAGATTTCATATGTGGATTGCCACCGAGAATCAGAAGGTTGGAGGAGAGAGCTCAGGGAAGAGCCAAGAAATCATCGAGTGTTCCATTCAGCTGGCTTTTTGGTAGAGAGATAGTCGTTTAA

Protein Analysis

399

Amino Acids

45.54

Weight (kDa)

5.86

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 71 - 392 3.6e-160 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02630 AT5G16230 AT5G16240
fragaria_vesca FvH4_6g00080
malus_domestica MD00G1017300.v1.1 MD12G1261600.v1.1
prunus_persica Prupe.6G365100_v2.0.a1 Prupe.6G365100_v2.0.a1 Prupe.6G365200_v2.0.a1
pyrus_communis pycom04g21990
rosa_chinensis RchiOBHm_Chr3g0447411
rosa_laevigata RLG00000026001
rosa_multiflora Rmu_ssc0000473.1_g000020
rosa_roxburghii Rroxscaffold_6G00425610
rosa_rugosa Rorug02G0601300
rosa_samantha Rh3AG001400 Rh3BG001100 Rh3CG001000 Rh3DG001300
rosa_wichuraiana Rw3G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 773
AccB7I CCANNNNNTGG 1 cut(s) 970
AciI CCGC 2 cut(s) 73, 967
AclWI GGATC 3 cut(s) 632, 634, 645
AcsI RAATTY 2 cut(s) 16, 372
AcuI CTGAAG 1 cut(s) 549
AcvI CACGTG 1 cut(s) 170
AfiI CCNNNNNNNGG 3 cut(s) 665, 757, 970
AflIII ACRYGT 1 cut(s) 178
AgsI TTSAA 2 cut(s) 185, 689
AjnI CCWGG 2 cut(s) 497, 725
AluBI AGCT 9 cut(s) 49, 425, 832, 866, 872, 956, 1073, 1127, 1167
AluI AGCT 9 cut(s) 49, 425, 832, 866, 872, 956, 1073, 1127, 1167
Alw21I GWGCWC 4 cut(s) 267, 948, 1075, 1129
Alw26I GTCTC 4 cut(s) 128, 207, 470, 540
Alw44I GTGCAC 1 cut(s) 263
AlwI GGATC 3 cut(s) 632, 634, 645
AlwNI CAGNNNCTG 1 cut(s) 970
AoxI GGCC 1 cut(s) 696
ApaLI GTGCAC 1 cut(s) 263
ApoI RAATTY 2 cut(s) 16, 372
Asp700I GAANNNNTTC 2 cut(s) 32, 426
AspS9I GGNCC 2 cut(s) 696, 748
AsuHPI GGTGA 4 cut(s) 35, 37, 484, 1070
AvaII GGWCC 1 cut(s) 748
BaeGI GKGCMC 1 cut(s) 267
BamHI GGATCC 1 cut(s) 637
BanI GGYRCC 1 cut(s) 773
BanII GRGCYC 2 cut(s) 1075, 1129
BbrPI CACGTG 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 4 cut(s) 267, 948, 1075, 1129
BccI CCATC 5 cut(s) 46, 455, 501, 914, 1020
BciT130I CCWGG 2 cut(s) 499, 727
BclI TGATCA 1 cut(s) 411
BcoDI GTCTC 4 cut(s) 128, 207, 470, 540
BfaI CTAG 2 cut(s) 458, 513
BglII AGATCT 2 cut(s) 83, 219
BisI GCNGC 1 cut(s) 968
BlsI GCNGC 1 cut(s) 969
Bme1390I CCNGG 2 cut(s) 499, 727
Bme18I GGWCC 1 cut(s) 748
BmgT120I GGNCC 2 cut(s) 696, 748
BmiI GGNNCC 3 cut(s) 639, 749, 775
BmrFI CCNGG 2 cut(s) 499, 727
BpiI GAAGAC 1 cut(s) 611
BplI GAGNNNNNCTC 4 cut(s) 209, 241, 935, 967
Bpu10I CCTNAGC 1 cut(s) 1128
BpuEI CTTGAG 2 cut(s) 236, 1059
BsaAI YACGTR 1 cut(s) 170
BsaI GGTCTC 1 cut(s) 540
BsaJI CCNNGG 7 cut(s) 21, 129, 498, 664, 712, 783, 987
Bsc4I CCNNNNNNNGG 3 cut(s) 665, 757, 970
Bse1I ACTGG 1 cut(s) 484
Bse3DI GCAATG 1 cut(s) 777
BseBI CCWGG 2 cut(s) 499, 727
BseDI CCNNGG 7 cut(s) 21, 129, 498, 664, 712, 783, 987
BseGI GGATG 3 cut(s) 227, 478, 793
BseLI CCNNNNNNNGG 3 cut(s) 665, 757, 970
BseMI GCAATG 1 cut(s) 777
BseMII CTCAG 6 cut(s) 22, 129, 309, 776, 966, 1142
BseNI ACTGG 1 cut(s) 484
BseRI GAGGAG 4 cut(s) 115, 251, 1052, 1133
BseSI GKGCMC 1 cut(s) 267
BseYI CCCAGC 1 cut(s) 963
BsgI GTGCAG 1 cut(s) 888
BshFI GGCC 1 cut(s) 698
BshNI GGYRCC 1 cut(s) 773
BsiHKAI GWGCWC 4 cut(s) 267, 948, 1075, 1129
BslFI GGGAC 2 cut(s) 55, 761
BslI CCNNNNNNNGG 3 cut(s) 665, 757, 970
BsmAI GTCTC 4 cut(s) 128, 207, 470, 540
BsmFI GGGAC 2 cut(s) 55, 761
BsnI GGCC 1 cut(s) 698
Bso31I GGTCTC 1 cut(s) 540
Bsp1286I GDGCHC 4 cut(s) 267, 948, 1075, 1129
Bsp143I GATC 5 cut(s) 83, 219, 411, 626, 637
Bsp19I CCATGG 1 cut(s) 712
BspACI CCGC 2 cut(s) 73, 967
BspANI GGCC 1 cut(s) 698
BspCNI CTCAG 6 cut(s) 21, 130, 310, 775, 965, 1141
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 3 cut(s) 639, 749, 775
BspPI GGATC 3 cut(s) 632, 634, 645
BspQI GCTCTTC 2 cut(s) 432, 1129
BspT107I GGYRCC 1 cut(s) 773
BspTNI GGTCTC 1 cut(s) 540
BsrDI GCAATG 1 cut(s) 777
BsrI ACTGG 1 cut(s) 484
BssECI CCNNGG 7 cut(s) 21, 129, 498, 664, 712, 783, 987
BssMI GATC 5 cut(s) 83, 219, 411, 626, 637
BssT1I CCWWGG 4 cut(s) 129, 664, 712, 987
Bst2UI CCWGG 2 cut(s) 499, 727
Bst4CI ACNGT 2 cut(s) 25, 859
Bst6I CTCTTC 4 cut(s) 117, 432, 525, 1129
BstAPI GCANNNNNTGC 1 cut(s) 524
BstBAI YACGTR 1 cut(s) 170
BstC8I GCNNGC 5 cut(s) 294, 487, 732, 903, 1169
BstDEI CTNAG 8 cut(s) 8, 138, 232, 318, 762, 862, 952, 1128
BstDSI CCRYGG 2 cut(s) 21, 712
BstF5I GGATG 3 cut(s) 227, 478, 793
BstKTI GATC 5 cut(s) 86, 222, 414, 629, 640
BstMAI GTCTC 4 cut(s) 128, 207, 470, 540
BstMBI GATC 5 cut(s) 83, 219, 411, 626, 637
BstMWI GCNNNNNNNGC 3 cut(s) 271, 524, 731
BstNI CCWGG 2 cut(s) 499, 727
BstNSI RCATGY 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 497, 725
BstSLI GKGCMC 1 cut(s) 267
BstV2I GAAGAC 1 cut(s) 611
BstX2I RGATCY 3 cut(s) 83, 219, 637
BstXI CCANNNNNNTGG 1 cut(s) 1167
BstYI RGATCY 3 cut(s) 83, 219, 637
BsuRI GGCC 1 cut(s) 698
BtgI CCRYGG 2 cut(s) 21, 712
BtsCI GGATG 3 cut(s) 227, 478, 793
BtsIMutI CAGTG 1 cut(s) 855
Cac8I GCNNGC 5 cut(s) 294, 487, 732, 903, 1169
CaiI CAGNNNCTG 1 cut(s) 970
CciI TCATGA 1 cut(s) 799
Cfr13I GGNCC 2 cut(s) 696, 748
CspCI CAANNNNNGTGG 2 cut(s) 804, 839
DdeI CTNAG 8 cut(s) 8, 138, 232, 318, 762, 862, 952, 1128
DpnI GATC 5 cut(s) 85, 221, 413, 628, 639
DpnII GATC 5 cut(s) 83, 219, 411, 626, 637
Eam1104I CTCTTC 4 cut(s) 117, 432, 525, 1129
EarI CTCTTC 4 cut(s) 117, 432, 525, 1129
Ecl136II GAGCTC 2 cut(s) 1073, 1127
Eco130I CCWWGG 4 cut(s) 129, 664, 712, 987
Eco24I GRGCYC 2 cut(s) 1075, 1129
Eco31I GGTCTC 1 cut(s) 540
Eco47I GGWCC 1 cut(s) 748
Eco53kI GAGCTC 2 cut(s) 1073, 1127
Eco57I CTGAAG 1 cut(s) 549
Eco72I CACGTG 1 cut(s) 170
EcoICRI GAGCTC 2 cut(s) 1073, 1127
EcoO109I RGGNCCY 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 497, 725
EcoT14I CCWWGG 4 cut(s) 129, 664, 712, 987
EcoT38I GRGCYC 2 cut(s) 1075, 1129
ErhI CCWWGG 4 cut(s) 129, 664, 712, 987
FalI AAGNNNNNCTT 4 cut(s) 291, 323, 411, 443
FaqI GGGAC 2 cut(s) 55, 761
FauI CCCGC 1 cut(s) 80
FauNDI CATATG 1 cut(s) 1085
FbaI TGATCA 1 cut(s) 411
Fnu4HI GCNGC 1 cut(s) 968
FokI GGATG 3 cut(s) 214, 485, 800
FriOI GRGCYC 2 cut(s) 1075, 1129
Fsp4HI GCNGC 1 cut(s) 968
FspBI CTAG 2 cut(s) 458, 513
GluI GCNGC 1 cut(s) 968
GsaI CCCAGC 1 cut(s) 967
HaeIII GGCC 1 cut(s) 698
HinfI GANTC 7 cut(s) 93, 122, 173, 190, 320, 330, 1104
HphI GGTGA 4 cut(s) 35, 37, 484, 1070
Hpy166II GTNNAC 2 cut(s) 28, 265
Hpy188I TCNGA 7 cut(s) 11, 83, 121, 325, 765, 787, 1109
Hpy8I GTNNAC 2 cut(s) 28, 265
HpyAV CCTTC 5 cut(s) 43, 66, 560, 712, 1104
HpyCH4III ACNGT 2 cut(s) 25, 859
HpyCH4IV ACGT 2 cut(s) 169, 796
HpyCH4V TGCA 3 cut(s) 265, 485, 905
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 524, 731
HpyF3I CTNAG 8 cut(s) 8, 138, 232, 318, 762, 862, 952, 1128
HpySE526I ACGT 2 cut(s) 169, 796
Ksp22I TGATCA 1 cut(s) 411
Kzo9I GATC 5 cut(s) 83, 219, 411, 626, 637
LguI GCTCTTC 2 cut(s) 432, 1129
MaeI CTAG 2 cut(s) 458, 513
MaeII ACGT 2 cut(s) 169, 796
MaeIII GTNAC 2 cut(s) 174, 187
MalI GATC 5 cut(s) 85, 221, 413, 628, 639
MboI GATC 5 cut(s) 83, 219, 411, 626, 637
MflI RGATCY 3 cut(s) 83, 219, 637
MhlI GDGCHC 4 cut(s) 267, 948, 1075, 1129
MluCI AATT 5 cut(s) 16, 372, 597, 609, 755
MlyI GAGTC 4 cut(s) 116, 182, 184, 329
MmeI TCCRAC 4 cut(s) 144, 382, 455, 1095
MroXI GAANNNNTTC 2 cut(s) 32, 426
MseI TTAA 2 cut(s) 450, 1195
MslI CAYNNNNRTG 1 cut(s) 260
MspA1I CMGCKG 3 cut(s) 956, 967, 1167
MspR9I CCNGG 2 cut(s) 499, 727
MvaI CCWGG 2 cut(s) 499, 727
MwoI GCNNNNNNNGC 3 cut(s) 271, 524, 731
NcoI CCATGG 1 cut(s) 712
NdeI CATATG 1 cut(s) 1085
NdeII GATC 5 cut(s) 83, 219, 411, 626, 637
NlaIV GGNNCC 3 cut(s) 639, 749, 775
NmuCI GTSAC 2 cut(s) 174, 187
NspI RCATGY 1 cut(s) 182
PagI TCATGA 1 cut(s) 799
PciI ACATGT 1 cut(s) 178
PciSI GCTCTTC 2 cut(s) 432, 1129
PdmI GAANNNNTTC 2 cut(s) 32, 426
PfeI GAWTC 3 cut(s) 93, 330, 1104
PflMI CCANNNNNTGG 1 cut(s) 970
PkrI GCNGC 1 cut(s) 969
PleI GAGTC 4 cut(s) 116, 181, 184, 328
PmaCI CACGTG 1 cut(s) 170
PmlI CACGTG 1 cut(s) 170
PpsI GAGTC 4 cut(s) 116, 181, 184, 328
Ppu21I YACGTR 1 cut(s) 170
PpuMI RGGWCCY 1 cut(s) 748
PscI ACATGT 1 cut(s) 178
Psp124BI GAGCTC 2 cut(s) 1075, 1129
Psp5II RGGWCCY 1 cut(s) 748
Psp6I CCWGG 2 cut(s) 497, 725
PspCI CACGTG 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 963
PspGI CCWGG 2 cut(s) 497, 725
PspN4I GGNNCC 3 cut(s) 639, 749, 775
PspPI GGNCC 2 cut(s) 696, 748
PspPPI RGGWCCY 1 cut(s) 748
PsrI GAACNNNNNNTAC 2 cut(s) 644, 676
PstNI CAGNNNCTG 1 cut(s) 970
PsuI RGATCY 3 cut(s) 83, 219, 637
PvuII CAGCTG 2 cut(s) 956, 1167
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 2 cut(s) 1075, 1129
SapI GCTCTTC 2 cut(s) 432, 1129
SaqAI TTAA 2 cut(s) 450, 1195
SatI GCNGC 1 cut(s) 968
Sau3AI GATC 5 cut(s) 83, 219, 411, 626, 637
Sau96I GGNCC 2 cut(s) 696, 748
SchI GAGTC 4 cut(s) 116, 182, 184, 329
ScrFI CCNGG 2 cut(s) 499, 727
SduI GDGCHC 4 cut(s) 267, 948, 1075, 1129
SinI GGWCC 1 cut(s) 748
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 2 cut(s) 215, 1074
SmoI CTYRAG 2 cut(s) 215, 1074
Sse9I AATT 5 cut(s) 16, 372, 597, 609, 755
SsiI CCGC 2 cut(s) 73, 967
SspMI CTAG 2 cut(s) 458, 513
SstI GAGCTC 2 cut(s) 1075, 1129
StyD4I CCNGG 2 cut(s) 497, 725
StyI CCWWGG 4 cut(s) 129, 664, 712, 987
TaaI ACNGT 2 cut(s) 25, 859
TaiI ACGT 2 cut(s) 172, 799
TaqI TCGA 3 cut(s) 279, 942, 1151
TasI AATT 5 cut(s) 16, 372, 597, 609, 755
TauI GCSGC 1 cut(s) 970
TfiI GAWTC 3 cut(s) 93, 330, 1104
Tru1I TTAA 2 cut(s) 450, 1195
Tru9I TTAA 2 cut(s) 450, 1195
TscAI CASTG 1 cut(s) 862
TseFI GTSAC 2 cut(s) 174, 187
Tsp45I GTSAC 2 cut(s) 174, 187
TspGWI ACGGA 1 cut(s) 662
TspRI CASTG 1 cut(s) 862
Van91I CCANNNNNTGG 1 cut(s) 970
VneI GTGCAC 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 748
XapI RAATTY 2 cut(s) 16, 372
XbaI TCTAGA 1 cut(s) 457
XceI RCATGY 1 cut(s) 182
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XmnI GAANNNNTTC 2 cut(s) 32, 426
XspI CTAG 2 cut(s) 458, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.