MD12G1261600.v1.1

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010828
Physical Location & Seq
Reverse (-)
32833463 .. 32838033
4571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1261600.v1.1.491

Sequence Viewer

Length: 1182 bp
ATGGCTGTGAGTCTGAATTCGATGCTGACCTTCCCGTCTCACAGCCTCACTGCCAAGTCCCACAATCTCAGATCTCCCAAAATATTCATGGCCTCCACTCTTCACTCCTCCACCAAAGAGACTGAGAATCTGAAGAAGTCTTTTGGCCCTCCGCGTGAGGTACATGTTCAGGTGACTCATTCAATGCCTCCACAACAGATTGAGATTTTCAAGTCATTAGAGGATTGGGCTGAGAATAACATCTTGGTGCACCTAAAGCCTGTTGAGAAATGTTGGCAACCACAAGATTTTCTTCCACTTCCCGAGTCAGATGGATTCGAGGAGCAAGTTAAGGAGTTGAGGGAAAGAGCAAGGGAAATTCCTGATGATTACTTTGTTGTGTTGGTTGGTGATATGATCACAGAAGAAGCCCTTCCAACGTACCAGACAATGCTTAATACCCTAGACGGAGTTCGGGATGAGACTGGTGCAAGCCTTACTTCCTGGGCTATTTGGACCAGGGCGTGGACTGCTGAAGAGAATAGGCATGGTGATCTTCTTAACAAGTATCTTTATCTCTCTGGACGAGTAGATATGAGACAAATCGAGAAAACAATTCAGTACCTTATTGGATCTGGAATGGATCCTAAAACAGAGAACAATCCCTACCTTGGGTTCATTTACACTTCATTCCAAGAGAGGGCAACATTCATCTCCCATGGGAACACAGCCAGGCAAGCCAAAGAGCATGGGGACCTGAAATTGGCTCAGATATGCGGCACCATTGCCTCGGATGAAAAGCGCCATGAAACTGCGTATACAAAGATTGTGGAAAAGCTGTTTGAGATTGATCCAGATGGCACTGTGTTGGCCTTAGCTGACATGATGAAGAAAAAGATATCAATGCCTGCCCACTTGATGTACGATGGTCAGGATGATAATCTGTTTGATCATTTCTCAGCTGTTGCCCAGAGGCTTGAGGTGTATACTGCCAGGGATTATGCTGATATATTGGAGTTTTTGGTTGGAAGATGGAATGTGGAGAAGTTGACTGGTCTTTCTGGCGAGGGACGCATGGCGCAAGATTTTGTGTGTAAATTGCCACAGAGAATCAGGAGGTTGGAGGAAAGAGCACAGGGAAGGGCCAAGGCTTCTTCCCTTGTTCCATTCAGCTGGATTTTTGGTAGGGAAGTAAAGGTTTAA

Protein Analysis

394

Amino Acids

44.92

Weight (kDa)

5.8

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 66 - 387 2.6e-160 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02630 AT5G16230 AT5G16240
fragaria_vesca FvH4_6g00080
malus_domestica MD00G1017300.v1.1 MD12G1261600.v1.1
prunus_persica Prupe.6G365100_v2.0.a1 Prupe.6G365100_v2.0.a1 Prupe.6G365200_v2.0.a1
pyrus_communis pycom04g21990
rosa_chinensis RchiOBHm_Chr3g0447411
rosa_laevigata RLG00000026001
rosa_multiflora Rmu_ssc0000473.1_g000020
rosa_roxburghii Rroxscaffold_6G00425610
rosa_rugosa Rorug02G0601300
rosa_samantha Rh3AG001400 Rh3BG001100 Rh3CG001000 Rh3DG001300
rosa_wichuraiana Rw3G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 34
AccB1I GGYRCC 1 cut(s) 758
AccB7I CCANNNNNTGG 1 cut(s) 504
AccI GTMKAC 2 cut(s) 797, 965
AccII CGCG 1 cut(s) 154
AciI CCGC 2 cut(s) 152, 756
AclWI GGATC 4 cut(s) 617, 619, 630, 824
AcsI RAATTY 2 cut(s) 16, 357
AcuI CTGAAG 2 cut(s) 152, 534
AfaI GTAC 4 cut(s) 162, 422, 602, 902
AfiI CCNNNNNNNGG 5 cut(s) 504, 650, 651, 679, 742
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 2 cut(s) 183, 211
AjnI CCWGG 4 cut(s) 482, 497, 710, 971
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AluBI AGCT 4 cut(s) 817, 857, 941, 1152
AluI AGCT 4 cut(s) 817, 857, 941, 1152
Alw21I GWGCWC 2 cut(s) 252, 1114
Alw26I GTCTC 4 cut(s) 42, 113, 455, 571
Alw44I GTGCAC 1 cut(s) 248
AlwI GGATC 4 cut(s) 617, 619, 630, 824
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 4 cut(s) 90, 145, 849, 1122
ApaLI GTGCAC 1 cut(s) 248
ApoI RAATTY 2 cut(s) 16, 357
Asp700I GAANNNNTTC 1 cut(s) 411
AspLEI GCGC 2 cut(s) 783, 1060
AspS9I GGNCC 4 cut(s) 146, 495, 733, 1122
AsuHPI GGTGA 3 cut(s) 184, 401, 542
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 2 cut(s) 495, 733
BaeGI GKGCMC 1 cut(s) 252
BamHI GGATCC 1 cut(s) 622
BanI GGYRCC 1 cut(s) 758
Bbv12I GWGCWC 2 cut(s) 252, 1114
BccI CCATC 4 cut(s) 305, 830, 899, 1005
BciT130I CCWGG 4 cut(s) 484, 499, 712, 973
BclI TGATCA 2 cut(s) 396, 928
BcoDI GTCTC 4 cut(s) 42, 113, 455, 571
BfaI CTAG 1 cut(s) 443
BfoI RGCGCY 1 cut(s) 784
BglII AGATCT 1 cut(s) 71
BisI GCNGC 1 cut(s) 757
BlsI GCNGC 1 cut(s) 758
Bme1390I CCNGG 4 cut(s) 484, 499, 712, 973
Bme18I GGWCC 2 cut(s) 495, 733
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 4 cut(s) 146, 495, 733, 1122
BmiI GGNNCC 3 cut(s) 624, 734, 760
BmrFI CCNGG 4 cut(s) 484, 499, 712, 973
BmsI GCATC 1 cut(s) 12
Bpu10I CCTNAGC 1 cut(s) 853
BpuEI CTTGAG 1 cut(s) 977
BsaBI GATNNNNATC 1 cut(s) 918
BsaJI CCNNGG 7 cut(s) 483, 498, 649, 697, 768, 972, 1125
Bsc4I CCNNNNNNNGG 5 cut(s) 504, 650, 651, 679, 742
Bse1I ACTGG 2 cut(s) 469, 1036
Bse3DI GCAATG 1 cut(s) 762
Bse8I GATNNNNATC 1 cut(s) 918
BseBI CCWGG 4 cut(s) 484, 499, 712, 973
BseDI CCNNGG 7 cut(s) 483, 498, 649, 697, 768, 972, 1125
BseGI GGATG 3 cut(s) 463, 778, 919
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 5 cut(s) 504, 650, 651, 679, 742
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 5 cut(s) 82, 114, 222, 761, 951
BseNI ACTGG 2 cut(s) 469, 1036
BseRI GAGGAG 2 cut(s) 97, 335
BseSI GKGCMC 1 cut(s) 252
Bsh1236I CGCG 1 cut(s) 154
BshFI GGCC 4 cut(s) 92, 147, 851, 1124
BshNI GGYRCC 1 cut(s) 758
BsiHKAI GWGCWC 2 cut(s) 252, 1114
BsiHKCI CYCGRG 1 cut(s) 302
BslFI GGGAC 3 cut(s) 43, 746, 1062
BslI CCNNNNNNNGG 5 cut(s) 504, 650, 651, 679, 742
BsmAI GTCTC 4 cut(s) 42, 113, 455, 571
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 3 cut(s) 43, 746, 1062
BsnI GGCC 4 cut(s) 92, 147, 851, 1124
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 2 cut(s) 252, 1114
Bsp143I GATC 7 cut(s) 71, 396, 532, 611, 622, 829, 928
Bsp19I CCATGG 1 cut(s) 697
BspACI CCGC 2 cut(s) 152, 756
BspANI GGCC 4 cut(s) 92, 147, 851, 1124
BspCNI CTCAG 5 cut(s) 81, 115, 223, 760, 950
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 3 cut(s) 624, 734, 760
BspPI GGATC 4 cut(s) 617, 619, 630, 824
BspT107I GGYRCC 1 cut(s) 758
BsrDI GCAATG 1 cut(s) 762
BsrI ACTGG 2 cut(s) 469, 1036
BssECI CCNNGG 7 cut(s) 483, 498, 649, 697, 768, 972, 1125
BssMI GATC 7 cut(s) 71, 396, 532, 611, 622, 829, 928
BssNAI GTATAC 2 cut(s) 798, 966
BssT1I CCWWGG 3 cut(s) 649, 697, 1125
Bst1107I GTATAC 2 cut(s) 798, 966
Bst2UI CCWGG 4 cut(s) 484, 499, 712, 973
Bst4CI ACNGT 1 cut(s) 844
Bst6I CTCTTC 2 cut(s) 105, 510
BstC8I GCNNGC 3 cut(s) 472, 717, 888
BstDEI CTNAG 6 cut(s) 68, 123, 231, 747, 853, 937
BstDSI CCRYGG 1 cut(s) 697
BstF5I GGATG 3 cut(s) 463, 778, 919
BstFNI CGCG 1 cut(s) 154
BstH2I RGCGCY 1 cut(s) 784
BstHHI GCGC 2 cut(s) 783, 1060
BstKTI GATC 7 cut(s) 74, 399, 535, 614, 625, 832, 931
BstMAI GTCTC 4 cut(s) 42, 113, 455, 571
BstMBI GATC 7 cut(s) 71, 396, 532, 611, 622, 829, 928
BstMWI GCNNNNNNNGC 4 cut(s) 256, 509, 716, 1050
BstNI CCWGG 4 cut(s) 484, 499, 712, 973
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 4 cut(s) 482, 497, 710, 971
BstSLI GKGCMC 1 cut(s) 252
BstUI CGCG 1 cut(s) 154
BstX2I RGATCY 3 cut(s) 71, 611, 622
BstXI CCANNNNNNTGG 1 cut(s) 1152
BstYI RGATCY 3 cut(s) 71, 611, 622
BstZ17I GTATAC 2 cut(s) 798, 966
BsuRI GGCC 4 cut(s) 92, 147, 851, 1124
BtgI CCRYGG 1 cut(s) 697
BtsCI GGATG 3 cut(s) 463, 778, 919
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 48, 840
Cac8I GCNNGC 3 cut(s) 472, 717, 888
CfoI GCGC 2 cut(s) 783, 1060
Cfr13I GGNCC 4 cut(s) 146, 495, 733, 1122
CseI GACGC 1 cut(s) 1059
Csp6I GTAC 4 cut(s) 161, 421, 601, 901
CspCI CAANNNNNGTGG 2 cut(s) 789, 824
CviAII CATG 8 cut(s) 88, 164, 527, 698, 728, 785, 862, 1054
CviQI GTAC 4 cut(s) 161, 421, 601, 901
DdeI CTNAG 6 cut(s) 68, 123, 231, 747, 853, 937
DpnI GATC 7 cut(s) 73, 398, 534, 613, 624, 831, 930
DpnII GATC 7 cut(s) 71, 396, 532, 611, 622, 829, 928
DrdI GACNNNNNNGTC 1 cut(s) 34
DseDI GACNNNNNNGTC 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 105, 510
EarI CTCTTC 2 cut(s) 105, 510
Eco130I CCWWGG 3 cut(s) 649, 697, 1125
Eco32I GATATC 1 cut(s) 879
Eco47I GGWCC 2 cut(s) 495, 733
Eco57I CTGAAG 2 cut(s) 152, 534
Eco88I CYCGRG 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 733
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 4 cut(s) 482, 497, 710, 971
EcoRV GATATC 1 cut(s) 879
EcoT14I CCWWGG 3 cut(s) 649, 697, 1125
ErhI CCWWGG 3 cut(s) 649, 697, 1125
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 8 cut(s) 91, 167, 530, 701, 731, 788, 865, 1057
FalI AAGNNNNNCTT 6 cut(s) 276, 308, 396, 428, 463, 495
FaqI GGGAC 3 cut(s) 43, 746, 1062
FatI CATG 8 cut(s) 87, 163, 526, 697, 727, 784, 861, 1053
FbaI TGATCA 2 cut(s) 396, 928
FblI GTMKAC 2 cut(s) 797, 965
Fnu4HI GCNGC 1 cut(s) 757
FokI GGATG 3 cut(s) 470, 785, 926
Fsp4HI GCNGC 1 cut(s) 757
FspBI CTAG 1 cut(s) 443
GlaI GCGC 2 cut(s) 782, 1059
GluI GCNGC 1 cut(s) 757
HaeII RGCGCY 1 cut(s) 784
HaeIII GGCC 4 cut(s) 92, 147, 851, 1124
HgaI GACGC 1 cut(s) 1059
HhaI GCGC 2 cut(s) 783, 1060
Hin1II CATG 8 cut(s) 91, 167, 530, 701, 731, 788, 865, 1057
Hin6I GCGC 2 cut(s) 781, 1058
HinP1I GCGC 2 cut(s) 781, 1058
HincII GTYRAC 1 cut(s) 1029
HindII GTYRAC 1 cut(s) 1029
HinfI GANTC 6 cut(s) 10, 127, 175, 305, 315, 1089
HphI GGTGA 3 cut(s) 184, 401, 542
Hpy166II GTNNAC 5 cut(s) 250, 507, 798, 966, 1029
Hpy188I TCNGA 6 cut(s) 15, 71, 132, 310, 750, 772
Hpy188III TCNNGA 9 cut(s) 302, 362, 455, 561, 586, 615, 833, 911, 1093
Hpy8I GTNNAC 5 cut(s) 250, 507, 798, 966, 1029
HpyAV CCTTC 3 cut(s) 40, 422, 1113
HpyCH4III ACNGT 1 cut(s) 844
HpyCH4IV ACGT 1 cut(s) 419
HpyCH4V TGCA 2 cut(s) 250, 470
HpyF10VI GCNNNNNNNGC 4 cut(s) 256, 509, 716, 1050
HpyF3I CTNAG 6 cut(s) 68, 123, 231, 747, 853, 937
HpySE526I ACGT 1 cut(s) 419
Hsp92II CATG 8 cut(s) 91, 167, 530, 701, 731, 788, 865, 1057
HspAI GCGC 2 cut(s) 781, 1058
Ksp22I TGATCA 2 cut(s) 396, 928
Kzo9I GATC 7 cut(s) 71, 396, 532, 611, 622, 829, 928
LmnI GCTCC 1 cut(s) 322
LweI GCATC 1 cut(s) 12
MaeI CTAG 1 cut(s) 443
MaeII ACGT 1 cut(s) 419
MaeIII GTNAC 1 cut(s) 172
MalI GATC 7 cut(s) 73, 398, 534, 613, 624, 831, 930
MboI GATC 7 cut(s) 71, 396, 532, 611, 622, 829, 928
MboII GAAGA 9 cut(s) 92, 145, 284, 416, 527, 527, 880, 1020, 1125
MflI RGATCY 3 cut(s) 71, 611, 622
MhlI GDGCHC 2 cut(s) 252, 1114
MluCI AATT 5 cut(s) 16, 357, 594, 740, 1076
MlyI GAGTC 3 cut(s) 19, 169, 314
MmeI TCCRAC 3 cut(s) 440, 985, 1080
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 4 cut(s) 330, 435, 540, 1180
MslI CAYNNNNRTG 1 cut(s) 245
MspA1I CMGCKG 2 cut(s) 941, 1152
MspR9I CCNGG 4 cut(s) 484, 499, 712, 973
MvaI CCWGG 4 cut(s) 484, 499, 712, 973
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 4 cut(s) 256, 509, 716, 1050
NcoI CCATGG 1 cut(s) 697
NdeII GATC 7 cut(s) 71, 396, 532, 611, 622, 829, 928
NlaIII CATG 8 cut(s) 91, 167, 530, 701, 731, 788, 865, 1057
NlaIV GGNNCC 3 cut(s) 624, 734, 760
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 1 cut(s) 167
PciI ACATGT 1 cut(s) 163
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 3 cut(s) 127, 315, 1089
PflMI CCANNNNNTGG 1 cut(s) 504
PkrI GCNGC 1 cut(s) 758
PleI GAGTC 3 cut(s) 18, 169, 313
PpsI GAGTC 3 cut(s) 18, 169, 313
PpuMI RGGWCCY 1 cut(s) 733
PscI ACATGT 1 cut(s) 163
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 4 cut(s) 482, 497, 710, 971
PspGI CCWGG 4 cut(s) 482, 497, 710, 971
PspN4I GGNNCC 3 cut(s) 624, 734, 760
PspPI GGNCC 4 cut(s) 146, 495, 733, 1122
PspPPI RGGWCCY 1 cut(s) 733
PsrI GAACNNNNNNTAC 2 cut(s) 629, 661
PsuI RGATCY 3 cut(s) 71, 611, 622
PvuII CAGCTG 2 cut(s) 941, 1152
RsaI GTAC 4 cut(s) 162, 422, 602, 902
RsaNI GTAC 4 cut(s) 161, 421, 601, 901
RseI CAYNNNNRTG 1 cut(s) 245
SaqAI TTAA 4 cut(s) 330, 435, 540, 1180
SatI GCNGC 1 cut(s) 757
Sau3AI GATC 7 cut(s) 71, 396, 532, 611, 622, 829, 928
Sau96I GGNCC 4 cut(s) 146, 495, 733, 1122
SchI GAGTC 3 cut(s) 19, 169, 314
ScrFI CCNGG 4 cut(s) 484, 499, 712, 973
SduI GDGCHC 2 cut(s) 252, 1114
SfaNI GCATC 1 cut(s) 12
SinI GGWCC 2 cut(s) 495, 733
SmiMI CAYNNNNRTG 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
Sse9I AATT 5 cut(s) 16, 357, 594, 740, 1076
SsiI CCGC 2 cut(s) 152, 756
SspI AATATT 1 cut(s) 84
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 4 cut(s) 482, 497, 710, 971
StyI CCWWGG 3 cut(s) 649, 697, 1125
TaaI ACNGT 1 cut(s) 844
TaiI ACGT 1 cut(s) 422
TaqI TCGA 3 cut(s) 20, 318, 585
TasI AATT 5 cut(s) 16, 357, 594, 740, 1076
TauI GCSGC 1 cut(s) 759
TfiI GAWTC 3 cut(s) 127, 315, 1089
Tru1I TTAA 4 cut(s) 330, 435, 540, 1180
Tru9I TTAA 4 cut(s) 330, 435, 540, 1180
TscAI CASTG 2 cut(s) 55, 847
TseFI GTSAC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 7 cut(s) 76, 646, 657, 679, 789, 801, 881
TspGWI ACGGA 1 cut(s) 462
TspRI CASTG 2 cut(s) 55, 847
Van91I CCANNNNNTGG 1 cut(s) 504
VneI GTGCAC 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 495, 733
XapI RAATTY 2 cut(s) 16, 357
XceI RCATGY 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmiI GTMKAC 2 cut(s) 797, 965
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.