RLG00000026001

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010828
Physical Location & Seq
Forward (+)
49768684 .. 49772459
3776 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026001

Sequence Viewer

Length: 1188 bp
ATGGCTCTCAGGCTCAACACCTACCCATCTCACACGCTCATCCCTTGTTCTTCTTCTTCTAAGTCCCGCAATTTCAGATCTCCAAAAATCTTCATGGCCTCCACTCCTTATTCCGTCTCCAGAGAGACTGAGAAGATGAAGAAAACTCAATTCCCTCCACGTGAGTCACACATTCAAGTGACTCATTCCATGCCTCCTGAACACCTTGAGATCTTCAAGTCCTTAGAGGAGTGGGCTGATGATAACATCTTGGTGCACCTAAAGCCTGTTGAGAAATGTTGGCAAGCACAAGATTTTCTTCCTGTCCCTGAGTCGGAGGGATTCTATGAGCAAGTGAAGGAGTTGAGGGAAAGAGCAAAAGAAATTCCTGATGATTATTTTGTTGTCTTGGTTGGAGATATGGTCACAGAAGAAGCTCTTCCAACATACCAGACAATGCTTAATACTCTAGATGGAGTTCGGGATGAGACTGGTGCAAGCCTCACCTCCTGGGCAAGATGGACCAGGGCATGGACTGCTGAAGAGAATAGGCATGGAGACCTTCTAAACAAGTATCTCTATCTCTCTGGACGAGTAGATATGAAACAAATCGAGAAGACAATTCAGTATCTTATTGGATCAGGAATGGATCCTAAAACGGAGAACAATCCCTACCTTGGTTTCATTTATACTTCATTTCAAGAGAGGGCTACCTTCATCTCCCATGGGAACACAGCCAGGCAAGCAAAAGAGCATGGGGACATGAAATTGGCTCAGATATGTGGCACCATTGCCTCGGATGAAAAGCGTCATGAAACTGCCTATACAAAGATTGTGGAGAAGCTCTTTGAGATAGACCCAGATGGCACTGTATTGGCTTTAGCTGACATGATGAAGAAAAAAATAACAATGCCTGCCCACTTGATGTATGATGGTCAGGATTATAATCTTTTCGAGAATTTCTCAGCTGTTGCCCAGCGGCTGGAAGTTTATACGGCCAAGGATTACTCTGATATTTTGGAGTTTCTGGTTGGTAGATGGAAAATAGAGGAGTTGACTGGTCTTTCTGGTGAGGGTCGTAGAGCACAAGATTTCGTATGTGGATTGCCACCGAGAATTAGAAGGTTGGAGGAGAGAGCTCAGGGAAGAGCCAAGAAATCATCCAGTGTTCCATTCAGCTGGCTTTTTGGTAGAGAGATAATTGTTTAA

Protein Analysis

396

Amino Acids

45.43

Weight (kDa)

6.0

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 68 - 389 1.2e-159 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02630 AT5G16230 AT5G16240
fragaria_vesca FvH4_6g00080
malus_domestica MD00G1017300.v1.1 MD12G1261600.v1.1
prunus_persica Prupe.6G365100_v2.0.a1 Prupe.6G365100_v2.0.a1 Prupe.6G365200_v2.0.a1
pyrus_communis pycom04g21990
rosa_chinensis RchiOBHm_Chr3g0447411
rosa_laevigata RLG00000026001
rosa_multiflora Rmu_ssc0000473.1_g000020
rosa_roxburghii Rroxscaffold_6G00425610
rosa_rugosa Rorug02G0601300
rosa_samantha Rh3AG001400 Rh3BG001100 Rh3CG001000 Rh3DG001300
rosa_wichuraiana Rw3G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 924
AccB1I GGYRCC 1 cut(s) 764
AccB7I CCANNNNNTGG 2 cut(s) 510, 961
AciI CCGC 2 cut(s) 67, 958
AclWI GGATC 3 cut(s) 623, 625, 636
AcoI YGGCCR 1 cut(s) 975
AcsI RAATTY 2 cut(s) 363, 937
AcuI CTGAAG 1 cut(s) 540
AcvI CACGTG 1 cut(s) 161
AfiI CCNNNNNNNGG 4 cut(s) 313, 510, 656, 961
AgsI TTSAA 3 cut(s) 176, 217, 680
AjnI CCWGG 3 cut(s) 488, 503, 716
AluBI AGCT 6 cut(s) 416, 823, 863, 947, 1118, 1158
AluI AGCT 6 cut(s) 416, 823, 863, 947, 1118, 1158
Alw21I GWGCWC 3 cut(s) 258, 1066, 1120
Alw26I GTCTC 4 cut(s) 119, 121, 461, 531
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 3 cut(s) 623, 625, 636
AlwNI CAGNNNCTG 1 cut(s) 961
AoxI GGCC 2 cut(s) 96, 975
ApaLI GTGCAC 1 cut(s) 254
ApoI RAATTY 2 cut(s) 363, 937
Asp700I GAANNNNTTC 1 cut(s) 417
AspS9I GGNCC 1 cut(s) 501
AsuHPI GGTGA 2 cut(s) 475, 1061
AvaII GGWCC 1 cut(s) 501
BaeGI GKGCMC 1 cut(s) 258
BamHI GGATCC 1 cut(s) 628
BanI GGYRCC 1 cut(s) 764
BanII GRGCYC 1 cut(s) 1120
BbrPI CACGTG 1 cut(s) 161
BbsI GAAGAC 1 cut(s) 602
Bbv12I GWGCWC 3 cut(s) 258, 1066, 1120
BccI CCATC 6 cut(s) 34, 446, 492, 836, 905, 1011
BceAI ACGGC 1 cut(s) 990
BciT130I CCWGG 3 cut(s) 490, 505, 718
BcoDI GTCTC 4 cut(s) 119, 121, 461, 531
BfaI CTAG 1 cut(s) 449
BglII AGATCT 2 cut(s) 77, 210
BisI GCNGC 1 cut(s) 959
BlsI GCNGC 1 cut(s) 960
Bme1390I CCNGG 3 cut(s) 490, 505, 718
Bme18I GGWCC 1 cut(s) 501
BmgT120I GGNCC 1 cut(s) 501
BmiI GGNNCC 2 cut(s) 630, 766
BmrFI CCNGG 3 cut(s) 490, 505, 718
BpiI GAAGAC 1 cut(s) 602
BplI GAGNNNNNCTC 2 cut(s) 926, 958
BpmI CTGGAG 1 cut(s) 103
Bpu10I CCTNAGC 1 cut(s) 1119
BpuEI CTTGAG 1 cut(s) 227
BsaAI YACGTR 1 cut(s) 161
BsaBI GATNNNNATC 1 cut(s) 924
BsaI GGTCTC 1 cut(s) 531
BsaJI CCNNGG 6 cut(s) 489, 504, 655, 703, 774, 978
BsaXI ACNNNNNCTCC 2 cut(s) 387, 417
Bsc4I CCNNNNNNNGG 4 cut(s) 313, 510, 656, 961
Bse1I ACTGG 3 cut(s) 475, 1042, 1143
Bse3DI GCAATG 1 cut(s) 768
Bse8I GATNNNNATC 1 cut(s) 924
BseBI CCWGG 3 cut(s) 490, 505, 718
BseDI CCNNGG 6 cut(s) 489, 504, 655, 703, 774, 978
BseGI GGATG 4 cut(s) 39, 469, 784, 1139
BseJI GATNNNNATC 1 cut(s) 924
BseLI CCNNNNNNNGG 4 cut(s) 313, 510, 656, 961
BseMI GCAATG 1 cut(s) 768
BseMII CTCAG 6 cut(s) 22, 120, 300, 767, 957, 1133
BseNI ACTGG 3 cut(s) 475, 1042, 1143
BseRI GAGGAG 3 cut(s) 242, 1043, 1124
BseSI GKGCMC 1 cut(s) 258
BseYI CCCAGC 1 cut(s) 954
BshFI GGCC 2 cut(s) 98, 977
BshNI GGYRCC 1 cut(s) 764
BsiHKAI GWGCWC 3 cut(s) 258, 1066, 1120
BslFI GGGAC 3 cut(s) 49, 290, 752
BslI CCNNNNNNNGG 4 cut(s) 313, 510, 656, 961
BsmAI GTCTC 4 cut(s) 119, 121, 461, 531
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 3 cut(s) 49, 290, 752
BsnI GGCC 2 cut(s) 98, 977
Bso31I GGTCTC 1 cut(s) 531
Bsp1286I GDGCHC 3 cut(s) 258, 1066, 1120
Bsp143I GATC 4 cut(s) 77, 210, 617, 628
Bsp19I CCATGG 1 cut(s) 703
BspACI CCGC 2 cut(s) 67, 958
BspANI GGCC 2 cut(s) 98, 977
BspCNI CTCAG 6 cut(s) 21, 121, 301, 766, 956, 1132
BspHI TCATGA 1 cut(s) 790
BspLI GGNNCC 2 cut(s) 630, 766
BspPI GGATC 3 cut(s) 623, 625, 636
BspQI GCTCTTC 2 cut(s) 423, 1120
BspT107I GGYRCC 1 cut(s) 764
BspTNI GGTCTC 1 cut(s) 531
BsrDI GCAATG 1 cut(s) 768
BsrI ACTGG 3 cut(s) 475, 1042, 1143
BssECI CCNNGG 6 cut(s) 489, 504, 655, 703, 774, 978
BssMI GATC 4 cut(s) 77, 210, 617, 628
BssT1I CCWWGG 3 cut(s) 655, 703, 978
Bst2UI CCWGG 3 cut(s) 490, 505, 718
Bst4CI ACNGT 1 cut(s) 850
Bst6I CTCTTC 3 cut(s) 423, 516, 1120
BstAPI GCANNNNNTGC 1 cut(s) 515
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 5 cut(s) 285, 478, 723, 894, 1160
BstDEI CTNAG 8 cut(s) 8, 60, 129, 223, 309, 753, 943, 1119
BstDSI CCRYGG 1 cut(s) 703
BstF5I GGATG 4 cut(s) 39, 469, 784, 1139
BstKTI GATC 4 cut(s) 80, 213, 620, 631
BstMAI GTCTC 4 cut(s) 119, 121, 461, 531
BstMBI GATC 4 cut(s) 77, 210, 617, 628
BstMWI GCNNNNNNNGC 3 cut(s) 262, 515, 722
BstNI CCWGG 3 cut(s) 490, 505, 718
BstSCI CCNGG 3 cut(s) 488, 503, 716
BstSLI GKGCMC 1 cut(s) 258
BstV2I GAAGAC 1 cut(s) 602
BstX2I RGATCY 3 cut(s) 77, 210, 628
BstXI CCANNNNNNTGG 1 cut(s) 1158
BstYI RGATCY 3 cut(s) 77, 210, 628
BsuRI GGCC 2 cut(s) 98, 977
BtgI CCRYGG 1 cut(s) 703
BtsCI GGATG 4 cut(s) 39, 469, 784, 1139
BtsIMutI CAGTG 2 cut(s) 846, 1150
Cac8I GCNNGC 5 cut(s) 285, 478, 723, 894, 1160
CaiI CAGNNNCTG 1 cut(s) 961
CciI TCATGA 1 cut(s) 790
Cfr13I GGNCC 1 cut(s) 501
CseI GACGC 1 cut(s) 776
CspCI CAANNNNNGTGG 2 cut(s) 795, 830
CviAII CATG 9 cut(s) 94, 190, 510, 533, 704, 734, 742, 791, 868
DdeI CTNAG 8 cut(s) 8, 60, 129, 223, 309, 753, 943, 1119
DpnI GATC 4 cut(s) 79, 212, 619, 630
DpnII GATC 4 cut(s) 77, 210, 617, 628
EaeI YGGCCR 1 cut(s) 975
Eam1104I CTCTTC 3 cut(s) 423, 516, 1120
EarI CTCTTC 3 cut(s) 423, 516, 1120
Ecl136II GAGCTC 1 cut(s) 1118
Eco130I CCWWGG 3 cut(s) 655, 703, 978
Eco24I GRGCYC 1 cut(s) 1120
Eco31I GGTCTC 1 cut(s) 531
Eco47I GGWCC 1 cut(s) 501
Eco53kI GAGCTC 1 cut(s) 1118
Eco57I CTGAAG 1 cut(s) 540
Eco72I CACGTG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 1118
EcoRII CCWGG 3 cut(s) 488, 503, 716
EcoT14I CCWWGG 3 cut(s) 655, 703, 978
EcoT38I GRGCYC 1 cut(s) 1120
ErhI CCWWGG 3 cut(s) 655, 703, 978
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 9 cut(s) 97, 193, 513, 536, 707, 737, 745, 794, 871
FalI AAGNNNNNCTT 4 cut(s) 282, 314, 402, 434
FaqI GGGAC 3 cut(s) 49, 290, 752
FatI CATG 9 cut(s) 93, 189, 509, 532, 703, 733, 741, 790, 867
FauI CCCGC 1 cut(s) 74
Fnu4HI GCNGC 1 cut(s) 959
FokI GGATG 4 cut(s) 26, 476, 791, 1126
FriOI GRGCYC 1 cut(s) 1120
Fsp4HI GCNGC 1 cut(s) 959
FspBI CTAG 1 cut(s) 449
GluI GCNGC 1 cut(s) 959
GsaI CCCAGC 1 cut(s) 958
GsuI CTGGAG 1 cut(s) 103
HaeIII GGCC 2 cut(s) 98, 977
HgaI GACGC 1 cut(s) 776
Hin1II CATG 9 cut(s) 97, 193, 513, 536, 707, 737, 745, 794, 871
HincII GTYRAC 1 cut(s) 1035
HindII GTYRAC 1 cut(s) 1035
HinfI GANTC 4 cut(s) 164, 181, 311, 321
HphI GGTGA 2 cut(s) 475, 1061
Hpy166II GTNNAC 2 cut(s) 256, 1035
Hpy188I TCNGA 5 cut(s) 77, 316, 756, 778, 991
Hpy8I GTNNAC 2 cut(s) 256, 1035
HpyAV CCTTC 4 cut(s) 331, 551, 703, 1095
HpyCH4III ACNGT 1 cut(s) 850
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 2 cut(s) 256, 476
HpyF10VI GCNNNNNNNGC 3 cut(s) 262, 515, 722
HpyF3I CTNAG 8 cut(s) 8, 60, 129, 223, 309, 753, 943, 1119
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 9 cut(s) 97, 193, 513, 536, 707, 737, 745, 794, 871
Kzo9I GATC 4 cut(s) 77, 210, 617, 628
LguI GCTCTTC 2 cut(s) 423, 1120
MaeI CTAG 1 cut(s) 449
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 3 cut(s) 165, 178, 403
MalI GATC 4 cut(s) 79, 212, 619, 630
MboI GATC 4 cut(s) 77, 210, 617, 628
MflI RGATCY 3 cut(s) 77, 210, 628
MhlI GDGCHC 3 cut(s) 258, 1066, 1120
MluCI AATT 8 cut(s) 70, 149, 363, 600, 746, 937, 1095, 1179
MlyI GAGTC 3 cut(s) 173, 175, 320
MmeI TCCRAC 4 cut(s) 294, 373, 446, 1086
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 2 cut(s) 441, 1186
MslI CAYNNNNRTG 2 cut(s) 176, 251
MspA1I CMGCKG 3 cut(s) 947, 958, 1158
MspR9I CCNGG 3 cut(s) 490, 505, 718
MvaI CCWGG 3 cut(s) 490, 505, 718
MwoI GCNNNNNNNGC 3 cut(s) 262, 515, 722
NcoI CCATGG 1 cut(s) 703
NdeII GATC 4 cut(s) 77, 210, 617, 628
NlaIII CATG 9 cut(s) 97, 193, 513, 536, 707, 737, 745, 794, 871
NlaIV GGNNCC 2 cut(s) 630, 766
NmuCI GTSAC 3 cut(s) 165, 178, 403
PagI TCATGA 1 cut(s) 790
PciSI GCTCTTC 2 cut(s) 423, 1120
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 1 cut(s) 321
PflMI CCANNNNNTGG 2 cut(s) 510, 961
PkrI GCNGC 1 cut(s) 960
PleI GAGTC 3 cut(s) 172, 175, 319
PmaCI CACGTG 1 cut(s) 161
PmlI CACGTG 1 cut(s) 161
PpsI GAGTC 3 cut(s) 172, 175, 319
Ppu21I YACGTR 1 cut(s) 161
PsiI TTATAA 1 cut(s) 924
Psp124BI GAGCTC 1 cut(s) 1120
Psp6I CCWGG 3 cut(s) 488, 503, 716
PspCI CACGTG 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 954
PspGI CCWGG 3 cut(s) 488, 503, 716
PspN4I GGNNCC 2 cut(s) 630, 766
PspPI GGNCC 1 cut(s) 501
PsrI GAACNNNNNNTAC 2 cut(s) 635, 667
PstNI CAGNNNCTG 1 cut(s) 961
PsuI RGATCY 3 cut(s) 77, 210, 628
PvuII CAGCTG 2 cut(s) 947, 1158
RseI CAYNNNNRTG 2 cut(s) 176, 251
SacI GAGCTC 1 cut(s) 1120
SapI GCTCTTC 2 cut(s) 423, 1120
SaqAI TTAA 2 cut(s) 441, 1186
SatI GCNGC 1 cut(s) 959
Sau3AI GATC 4 cut(s) 77, 210, 617, 628
Sau96I GGNCC 1 cut(s) 501
SchI GAGTC 3 cut(s) 173, 175, 320
ScrFI CCNGG 3 cut(s) 490, 505, 718
SduI GDGCHC 3 cut(s) 258, 1066, 1120
SinI GGWCC 1 cut(s) 501
SmiMI CAYNNNNRTG 2 cut(s) 176, 251
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 8 cut(s) 70, 149, 363, 600, 746, 937, 1095, 1179
SsiI CCGC 2 cut(s) 67, 958
SspMI CTAG 1 cut(s) 449
SstI GAGCTC 1 cut(s) 1120
StyD4I CCNGG 3 cut(s) 488, 503, 716
StyI CCWWGG 3 cut(s) 655, 703, 978
TaaI ACNGT 1 cut(s) 850
TaiI ACGT 1 cut(s) 163
TaqI TCGA 2 cut(s) 591, 933
TasI AATT 8 cut(s) 70, 149, 363, 600, 746, 937, 1095, 1179
TauI GCSGC 1 cut(s) 961
TfiI GAWTC 1 cut(s) 321
Tru1I TTAA 2 cut(s) 441, 1186
Tru9I TTAA 2 cut(s) 441, 1186
TscAI CASTG 2 cut(s) 853, 1150
TseFI GTSAC 3 cut(s) 165, 178, 403
Tsp45I GTSAC 3 cut(s) 165, 178, 403
TspGWI ACGGA 2 cut(s) 103, 653
TspRI CASTG 2 cut(s) 853, 1150
Van91I CCANNNNNTGG 2 cut(s) 510, 961
VneI GTGCAC 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 501
XapI RAATTY 2 cut(s) 363, 937
XbaI TCTAGA 1 cut(s) 448
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.