AT5G16230

Acyl- acyl-carrier-protein desaturase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010828
Physical Location & Seq
Forward (+)
5302905 .. 5306088
3184 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16230.2

Sequence Viewer

Length: 1206 bp
ATGTCGATGGCTTTGCTTTTGACATCGCCGGCTATGAAGCAGAAGCCGGCGGTGATAACTTCTCCTCGCCGTGGTTCTTCTCCTTCTCGTCGTCTTCGAGTTTCTTGTGTTACCACAAACCCTGCTAGGAAGAAAAACGAAACATGCAATCATTTTCGACCTATCAAAGAAGTAAATAACCAATTAACACACACAATACCACAGGAGAAGCTTGAGATCTTCAAATCAATGGAAAATTGGGCAGAACAAAAACTACTACCTTATCTCAAACCGGTAGAAGATTCATGGCAACCACAAGACTTCTTACCTGCGCCAGAGAACGACGACGAATTCTACGACCGAGTAAAAGAGATCAGAGAAAGAACAAAAGAGATACCTGACGATTACTTTGTAGTTCTTGTGGGAGATATGATCACAGAAGAAGCACTTCCAACGTATCAAACGACGTTAAACACACTAGACGGTGTTAAGGATGAAACCGGTGGGAGTTTATCGCCGTGGGCTGTGTGGATTAGAGCCTGGACGGCGGAGGAAAACCGTCACGGTGACTTACTCAACAAGTATCTTTATCTAACTGGTCGTGTTGATATGCGACATGTTGAGAAGACTATACAATATCTTATTGGTTCTGGTATGGATTCGAAATTTGAGAACAATCCATACAATGGATTCATCTACACATCATTCCAAGAGCGAGCAACATTCATCTCTCACGGCAACACGGCTAGGCTAGCCACAACATACGGCGATGTTACCCTCGCGAAGATCTGCGGCACAATCGCTGCCGACGAGAAGCGACACGAGACGGCGTACACGAAGATAGTGGAGAAGCTATTCGAGATCGACCCTGACGGATCTGTTCAGGCGTTAGCGAGTATGATGAAGAAACGGATCACAATGCCGGCTCATCTTATGCACGACGGAAGAGATAACGATTTGTTTGATCATTACGCCGCCGTGGCGCAGCGGATTGGAGTTTACACGGCGGCGGATTACGCTGGGATACTTGAGTTTCTGTTGCGGCGGTGGAAGGTGGAGAGTTTGGGATTGGGGTTATCAGGAGAAGGAAGGAGAGCACAGGAGTACTTGTGCACCTTGCCGCAGAGGATCAAGAGGTTAGAGGAAAGAGCTAATGATAGGGTCAAACTTGTGTCAAAACCTTCTGTTTCGTTTAGCTGGGTTTTTGGTAGAGATGTGAAACTATAG

Protein Analysis

401

Amino Acids

45.86

Weight (kDa)

7.68

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 1 - 319 9.1e-152 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02630 AT5G16230 AT5G16240
fragaria_vesca FvH4_6g00080
malus_domestica MD00G1017300.v1.1 MD12G1261600.v1.1
prunus_persica Prupe.6G365100_v2.0.a1 Prupe.6G365100_v2.0.a1 Prupe.6G365200_v2.0.a1
pyrus_communis pycom04g21990
rosa_chinensis RchiOBHm_Chr3g0447411
rosa_laevigata RLG00000026001
rosa_multiflora Rmu_ssc0000473.1_g000020
rosa_roxburghii Rroxscaffold_6G00425610
rosa_rugosa Rorug02G0601300
rosa_samantha Rh3AG001400 Rh3BG001100 Rh3CG001000 Rh3DG001300
rosa_wichuraiana Rw3G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 316
AccB7I CCANNNNNTGG 1 cut(s) 665
AccII CGCG 1 cut(s) 761
AclWI GGATC 3 cut(s) 862, 899, 1115
AcsI RAATTY 2 cut(s) 329, 644
AfaI GTAC 2 cut(s) 812, 1085
AfiI CCNNNNNNNGG 2 cut(s) 71, 665
AflIII ACRYGT 1 cut(s) 595
AgeI ACCGGT 2 cut(s) 271, 479
AgsI TTSAA 1 cut(s) 223
AjnI CCWGG 1 cut(s) 518
AluBI AGCT 4 cut(s) 211, 832, 1130, 1176
AluI AGCT 4 cut(s) 211, 832, 1130, 1176
Alw21I GWGCWC 2 cut(s) 1078, 1094
Alw26I GTCTC 1 cut(s) 797
Alw44I GTGCAC 1 cut(s) 1090
AlwI GGATC 3 cut(s) 862, 899, 1115
ApaLI GTGCAC 1 cut(s) 1090
ApeKI GCWGC 2 cut(s) 782, 964
ApoI RAATTY 2 cut(s) 329, 644
ArsI GACNNNNNNTTYG 2 cut(s) 371, 403
AsiGI ACCGGT 2 cut(s) 271, 479
Asp700I GAANNNNTTC 2 cut(s) 426, 833
AspLEI GCGC 2 cut(s) 313, 964
AsuHPI GGTGA 2 cut(s) 64, 557
AsuII TTCGAA 1 cut(s) 641
AsuNHI GCTAGC 1 cut(s) 730
BaeGI GKGCMC 1 cut(s) 1094
BaeI ACNNNNGTAYC 2 cut(s) 995, 1028
BauI CACGAG 1 cut(s) 800
BbsI GAAGAC 2 cut(s) 86, 611
Bbv12I GWGCWC 2 cut(s) 1078, 1094
BbvI GCAGC 2 cut(s) 769, 976
BceAI ACGGC 9 cut(s) 54, 481, 540, 730, 738, 760, 822, 941, 999
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 520
BciVI GTATCC 1 cut(s) 996
BclI TGATCA 2 cut(s) 411, 943
BcoDI GTCTC 1 cut(s) 797
BfaI CTAG 4 cut(s) 126, 458, 726, 731
BfmI CTRYAG 1 cut(s) 1202
BfuAI ACCTGC 1 cut(s) 316
BfuI GTATCC 1 cut(s) 996
BglI GCCNNNNNGGC 2 cut(s) 524, 959
BglII AGATCT 2 cut(s) 216, 765
BisI GCNGC 7 cut(s) 772, 783, 954, 965, 987, 1022, 1100
BlsI GCNGC 7 cut(s) 773, 784, 955, 966, 988, 1023, 1101
BmcAI AGTACT 1 cut(s) 1085
Bme1390I CCNGG 1 cut(s) 520
BmrFI CCNGG 1 cut(s) 520
BmtI GCTAGC 1 cut(s) 734
BpiI GAAGAC 2 cut(s) 86, 611
Bpu14I TTCGAA 1 cut(s) 641
BpuEI CTTGAG 2 cut(s) 233, 1028
BsaJI CCNNGG 3 cut(s) 70, 497, 957
BsaWI WCCGGW 2 cut(s) 271, 479
BsaXI ACNNNNNCTCC 2 cut(s) 1073, 1103
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 665
Bse118I RCCGGY 5 cut(s) 28, 46, 271, 479, 901
Bse1I ACTGG 1 cut(s) 580
BseBI CCWGG 1 cut(s) 520
BseDI CCNNGG 3 cut(s) 70, 497, 957
BseGI GGATG 1 cut(s) 478
BseLI CCNNNNNNNGG 2 cut(s) 71, 665
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 1 cut(s) 54
BseSI GKGCMC 1 cut(s) 1094
BseXI GCAGC 2 cut(s) 769, 976
BseYI CCCAGC 2 cut(s) 998, 1176
Bsh1236I CGCG 1 cut(s) 761
Bsh1285I CGRYCG 1 cut(s) 340
BshTI ACCGGT 2 cut(s) 271, 479
BsiEI CGRYCG 1 cut(s) 340
BsiHKAI GWGCWC 2 cut(s) 1078, 1094
BsiSI CCGG 5 cut(s) 29, 47, 272, 480, 902
BslI CCNNNNNNNGG 2 cut(s) 71, 665
BsmAI GTCTC 1 cut(s) 797
BsmBI CGTCTC 1 cut(s) 797
Bsp119I TTCGAA 1 cut(s) 641
Bsp1286I GDGCHC 2 cut(s) 1078, 1094
Bsp143I GATC 9 cut(s) 216, 351, 411, 765, 840, 854, 891, 943, 1107
Bsp68I TCGCGA 1 cut(s) 761
BspFNI CGCG 1 cut(s) 761
BspMI ACCTGC 1 cut(s) 316
BspOI GCTAGC 1 cut(s) 734
BspPI GGATC 3 cut(s) 862, 899, 1115
BspT104I TTCGAA 1 cut(s) 641
BsrFI RCCGGY 5 cut(s) 28, 46, 271, 479, 901
BsrI ACTGG 1 cut(s) 580
BssAI RCCGGY 5 cut(s) 28, 46, 271, 479, 901
BssECI CCNNGG 3 cut(s) 70, 497, 957
BssMI GATC 9 cut(s) 216, 351, 411, 765, 840, 854, 891, 943, 1107
BssSI CACGAG 1 cut(s) 800
Bst2BI CACGAG 1 cut(s) 800
Bst2UI CCWGG 1 cut(s) 520
Bst4CI ACNGT 3 cut(s) 464, 539, 545
Bst6I CTCTTC 1 cut(s) 919
BstBI TTCGAA 1 cut(s) 641
BstC8I GCNNGC 5 cut(s) 30, 48, 696, 732, 903
BstDSI CCRYGG 3 cut(s) 70, 497, 957
BstF5I GGATG 1 cut(s) 478
BstFNI CGCG 1 cut(s) 761
BstHHI GCGC 2 cut(s) 313, 964
BstKTI GATC 9 cut(s) 219, 354, 414, 768, 843, 857, 894, 946, 1110
BstMAI GTCTC 1 cut(s) 797
BstMBI GATC 9 cut(s) 216, 351, 411, 765, 840, 854, 891, 943, 1107
BstMCI CGRYCG 1 cut(s) 340
BstMWI GCNNNNNNNGC 4 cut(s) 524, 731, 959, 995
BstNI CCWGG 1 cut(s) 520
BstNSI RCATGY 2 cut(s) 147, 599
BstSCI CCNGG 1 cut(s) 518
BstSFI CTRYAG 1 cut(s) 1202
BstSLI GKGCMC 1 cut(s) 1094
BstUI CGCG 1 cut(s) 761
BstV1I GCAGC 2 cut(s) 769, 976
BstV2I GAAGAC 2 cut(s) 86, 611
BstX2I RGATCY 3 cut(s) 216, 765, 854
BstYI RGATCY 3 cut(s) 216, 765, 854
BsuI GTATCC 1 cut(s) 996
BtgI CCRYGG 3 cut(s) 70, 497, 957
BtgZI GCGATG 2 cut(s) 9, 762
BtsCI GGATG 1 cut(s) 478
BtuMI TCGCGA 1 cut(s) 761
BveI ACCTGC 1 cut(s) 316
Cac8I GCNNGC 5 cut(s) 30, 48, 696, 732, 903
CfoI GCGC 2 cut(s) 313, 964
Cfr10I RCCGGY 5 cut(s) 28, 46, 271, 479, 901
Csp6I GTAC 2 cut(s) 811, 1084
CspAI ACCGGT 2 cut(s) 271, 479
CviAII CATG 3 cut(s) 144, 285, 596
CviQI GTAC 2 cut(s) 811, 1084
DpnI GATC 9 cut(s) 218, 353, 413, 767, 842, 856, 893, 945, 1109
DpnII GATC 9 cut(s) 216, 351, 411, 765, 840, 854, 891, 943, 1107
Eam1104I CTCTTC 1 cut(s) 919
EarI CTCTTC 1 cut(s) 919
EciI GGCGGA 2 cut(s) 542, 1004
EcoRI GAATTC 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 518
Esp3I CGTCTC 1 cut(s) 797
FaeI CATG 3 cut(s) 147, 288, 599
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FatI CATG 3 cut(s) 143, 284, 595
FbaI TGATCA 2 cut(s) 411, 943
Fnu4HI GCNGC 7 cut(s) 772, 783, 954, 965, 987, 1022, 1100
FokI GGATG 1 cut(s) 485
Fsp4HI GCNGC 7 cut(s) 772, 783, 954, 965, 987, 1022, 1100
FspBI CTAG 4 cut(s) 126, 458, 726, 731
GlaI GCGC 2 cut(s) 312, 963
GluI GCNGC 7 cut(s) 772, 783, 954, 965, 987, 1022, 1100
GsaI CCCAGC 2 cut(s) 1002, 1180
HapII CCGG 5 cut(s) 29, 47, 272, 480, 902
HhaI GCGC 2 cut(s) 313, 964
Hin1II CATG 3 cut(s) 147, 288, 599
Hin6I GCGC 2 cut(s) 311, 962
HinP1I GCGC 2 cut(s) 311, 962
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 3 cut(s) 281, 638, 669
HpaII CCGG 5 cut(s) 29, 47, 272, 480, 902
HphI GGTGA 2 cut(s) 64, 557
Hpy166II GTNNAC 3 cut(s) 813, 979, 1092
Hpy188I TCNGA 1 cut(s) 356
Hpy188III TCNNGA 4 cut(s) 760, 838, 1059, 1111
Hpy8I GTNNAC 3 cut(s) 813, 979, 1092
Hpy99I CGWCG 6 cut(s) 93, 326, 329, 448, 791, 923
HpyAV CCTTC 5 cut(s) 93, 1024, 1058, 1062, 1170
HpyCH4III ACNGT 3 cut(s) 464, 539, 545
HpyCH4IV ACGT 2 cut(s) 434, 446
HpyCH4V TGCA 3 cut(s) 147, 916, 1092
HpyF10VI GCNNNNNNNGC 4 cut(s) 524, 731, 959, 995
HpySE526I ACGT 2 cut(s) 434, 446
Hsp92II CATG 3 cut(s) 147, 288, 599
HspAI GCGC 2 cut(s) 311, 962
KroI GCCGGC 3 cut(s) 28, 46, 901
KroNI GCCGGC 3 cut(s) 30, 48, 903
Ksp22I TGATCA 2 cut(s) 411, 943
Kzo9I GATC 9 cut(s) 216, 351, 411, 765, 840, 854, 891, 943, 1107
Lsp1109I GCAGC 2 cut(s) 769, 976
MaeI CTAG 4 cut(s) 126, 458, 726, 731
MaeII ACGT 2 cut(s) 434, 446
MaeIII GTNAC 4 cut(s) 109, 539, 545, 751
MalI GATC 9 cut(s) 218, 353, 413, 767, 842, 856, 893, 945, 1109
MboI GATC 9 cut(s) 216, 351, 411, 765, 840, 854, 891, 943, 1107
MflI RGATCY 3 cut(s) 216, 765, 854
MhlI GDGCHC 2 cut(s) 1078, 1094
MluCI AATT 4 cut(s) 182, 235, 329, 644
MmeI TCCRAC 1 cut(s) 455
MnlI CCTC 6 cut(s) 75, 523, 767, 1098, 1107, 1114
MroNI GCCGGC 3 cut(s) 28, 46, 901
MroXI GAANNNNTTC 2 cut(s) 426, 833
MseI TTAA 3 cut(s) 185, 449, 468
MspA1I CMGCKG 1 cut(s) 967
MspI CCGG 5 cut(s) 29, 47, 272, 480, 902
MspR9I CCNGG 1 cut(s) 520
MvaI CCWGG 1 cut(s) 520
MvnI CGCG 1 cut(s) 761
MwoI GCNNNNNNNGC 4 cut(s) 524, 731, 959, 995
NaeI GCCGGC 3 cut(s) 30, 48, 903
NdeII GATC 9 cut(s) 216, 351, 411, 765, 840, 854, 891, 943, 1107
NgoMIV GCCGGC 3 cut(s) 28, 46, 901
NheI GCTAGC 1 cut(s) 730
NlaIII CATG 3 cut(s) 147, 288, 599
NmuCI GTSAC 2 cut(s) 539, 545
NruI TCGCGA 1 cut(s) 761
NspI RCATGY 2 cut(s) 147, 599
NspV TTCGAA 1 cut(s) 641
PciI ACATGT 1 cut(s) 595
PcsI WCGNNNNNNNCGW 5 cut(s) 94, 333, 440, 786, 812
PdiI GCCGGC 3 cut(s) 30, 48, 903
PdmI GAANNNNTTC 2 cut(s) 426, 833
PfeI GAWTC 3 cut(s) 281, 638, 669
PflMI CCANNNNNTGG 1 cut(s) 665
PinAI ACCGGT 2 cut(s) 271, 479
PkrI GCNGC 7 cut(s) 773, 784, 955, 966, 988, 1023, 1101
PscI ACATGT 1 cut(s) 595
Psp6I CCWGG 1 cut(s) 518
PspFI CCCAGC 2 cut(s) 998, 1176
PspGI CCWGG 1 cut(s) 518
PsrI GAACNNNNNNTAC 4 cut(s) 237, 269, 644, 676
PsuI RGATCY 3 cut(s) 216, 765, 854
RruI TCGCGA 1 cut(s) 761
RsaI GTAC 2 cut(s) 812, 1085
RsaNI GTAC 2 cut(s) 811, 1084
SaqAI TTAA 3 cut(s) 185, 449, 468
SatI GCNGC 7 cut(s) 772, 783, 954, 965, 987, 1022, 1100
Sau3AI GATC 9 cut(s) 216, 351, 411, 765, 840, 854, 891, 943, 1107
ScaI AGTACT 1 cut(s) 1085
ScrFI CCNGG 1 cut(s) 520
SduI GDGCHC 2 cut(s) 1078, 1094
SfcI CTRYAG 1 cut(s) 1202
SfuI TTCGAA 1 cut(s) 641
SmlI CTYRAG 2 cut(s) 212, 1007
SmoI CTYRAG 2 cut(s) 212, 1007
Sse9I AATT 4 cut(s) 182, 235, 329, 644
SspMI CTAG 4 cut(s) 126, 458, 726, 731
StyD4I CCNGG 1 cut(s) 518
TaaI ACNGT 3 cut(s) 464, 539, 545
TaiI ACGT 2 cut(s) 437, 449
TaqI TCGA 6 cut(s) 5, 97, 157, 641, 837, 843
TaqII GACCGA 1 cut(s) 354
TasI AATT 4 cut(s) 182, 235, 329, 644
TatI WGTACW 1 cut(s) 1083
TauI GCSGC 5 cut(s) 774, 956, 989, 1024, 1102
TfiI GAWTC 3 cut(s) 281, 638, 669
Tru1I TTAA 3 cut(s) 185, 449, 468
Tru9I TTAA 3 cut(s) 185, 449, 468
TseFI GTSAC 2 cut(s) 539, 545
TseI GCWGC 2 cut(s) 782, 964
Tsp45I GTSAC 2 cut(s) 539, 545
TspDTI ATGAA 6 cut(s) 50, 273, 489, 661, 694, 896
TspGWI ACGGA 3 cut(s) 867, 904, 936
Van91I CCANNNNNTGG 1 cut(s) 665
VneI GTGCAC 1 cut(s) 1090
XapI RAATTY 2 cut(s) 329, 644
XceI RCATGY 2 cut(s) 147, 599
XmnI GAANNNNTTC 2 cut(s) 426, 833
XspI CTAG 4 cut(s) 126, 458, 726, 731
ZrmI AGTACT 1 cut(s) 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.