Rh3AG001400

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0010828
Physical Location & Seq
Reverse (-)
145703 .. 150273
4571 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG001400.1

Sequence Viewer

Length: 1092 bp
ATGATCATTTCTTCTGCTTGTTATAGAGAGACTGAGAAGATGAAGAAAACTCAATTCCCTCCACGTGAGTCACACATTCAAGTGACTCATTCCATGCCTCCTGAACACCTTGAGATCTTCAAGTCCTTAGAGGAGTGGGCTGATGATAACATCTTGGTGCACCTAAAGCCTGTTGAGAAATGTTGGCAAGCACAAGATTTTCTTCCTGCCCCTGAGTCGGAGGGATTCTATGAGCAAGTAAAGGAGTTGAGGGAAAGAGCAAAAGAAATTCCTGATGATTATTTTGTTGTCTTGGTTGGAGATATGGTCACAGAAGAAGCTCTTCCAACATACCAGACAATGCTTAATACTCTAGATGGAGTTCGGGATGAGACTGGTGCAAGCCTCACCTCCTGGGCAAGATGGACCAGGGCATGGACTGCTGAAGAGAATAGGCATGGAGACCTTCTAAACAAGTATCTCTATCTCTCTGGACGAGTAGATATGAAACAAATCGAGAAGACAATTCAGTATCTTATTGGATCAGGAATGGATCCTAAAACGGAGAACAATCCCTACCTTGGTTTCATTTATACTTCATTTCAAGAGAGGGCTACCTTCATCTCCCATGGGAACACAGCCAGGCAAGCAAAAGAGCATGGGGACGTGAAATTGGCTCAGATATGTGGCACCATTGCCTCGGATGAAAAACGTCATGAAACTGCCTATACAAAGATTGTGGAGAAGCTCTTTGAGATAGACCCAGATGGCACTGTATTGGCTTTAGCTGACATGATGAAGAAAAAAATAACAATGCCTGCCCACTTGATGTATGATGGTCAGGATTATAATCTTTTCGAGAATTTCTCAGCTGTTGCCCAGCGGCTGGAAGTTTATACGGCCAAGGATTATGCTGATATTTTGGAGTTTCTGGTTGGTAGATGGAAAATAGAGGAGTTGACTGGTCTTTCTGGTGAGGGTCGTAGAGCACAAGATTTCGTATGTGGATTGCCACCGAGAATTAGAAGGTTGGAGGAGAGAGCTCAGGGAAGAGCCAAGAAATCATCCAGTGTTCCATTCAGCTGGCTTTTTGGTAGAGAGATAATTGTTTAA

Protein Analysis

363

Amino Acids

41.78

Weight (kDa)

5.45

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 36 - 357 5.3e-160 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02630 AT5G16230 AT5G16240
fragaria_vesca FvH4_6g00080
malus_domestica MD00G1017300.v1.1 MD12G1261600.v1.1
prunus_persica Prupe.6G365100_v2.0.a1 Prupe.6G365100_v2.0.a1 Prupe.6G365200_v2.0.a1
pyrus_communis pycom04g21990
rosa_chinensis RchiOBHm_Chr3g0447411
rosa_laevigata RLG00000026001
rosa_multiflora Rmu_ssc0000473.1_g000020
rosa_roxburghii Rroxscaffold_6G00425610
rosa_rugosa Rorug02G0601300
rosa_samantha Rh3AG001400 Rh3BG001100 Rh3CG001000 Rh3DG001300
rosa_wichuraiana Rw3G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
AccB1I GGYRCC 1 cut(s) 668
AccB7I CCANNNNNTGG 2 cut(s) 414, 865
AciI CCGC 1 cut(s) 862
AclWI GGATC 3 cut(s) 527, 529, 540
AcoI YGGCCR 1 cut(s) 879
AcsI RAATTY 2 cut(s) 267, 841
AcuI CTGAAG 1 cut(s) 444
AcvI CACGTG 1 cut(s) 65
AfiI CCNNNNNNNGG 4 cut(s) 217, 414, 560, 865
AgsI TTSAA 3 cut(s) 80, 121, 584
AjiI CACGTC 1 cut(s) 646
AjnI CCWGG 3 cut(s) 392, 407, 620
AluBI AGCT 6 cut(s) 320, 727, 767, 851, 1022, 1062
AluI AGCT 6 cut(s) 320, 727, 767, 851, 1022, 1062
Alw21I GWGCWC 3 cut(s) 162, 970, 1024
Alw26I GTCTC 3 cut(s) 23, 365, 435
Alw44I GTGCAC 1 cut(s) 158
AlwI GGATC 3 cut(s) 527, 529, 540
AlwNI CAGNNNCTG 1 cut(s) 865
AoxI GGCC 1 cut(s) 879
ApaLI GTGCAC 1 cut(s) 158
ApoI RAATTY 2 cut(s) 267, 841
Asp700I GAANNNNTTC 1 cut(s) 321
AspS9I GGNCC 1 cut(s) 405
AsuHPI GGTGA 2 cut(s) 379, 965
AvaII GGWCC 1 cut(s) 405
BaeGI GKGCMC 1 cut(s) 162
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 668
BanII GRGCYC 1 cut(s) 1024
BbrPI CACGTG 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 506
Bbv12I GWGCWC 3 cut(s) 162, 970, 1024
BccI CCATC 5 cut(s) 350, 396, 740, 809, 915
BceAI ACGGC 1 cut(s) 894
BciT130I CCWGG 3 cut(s) 394, 409, 622
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 3 cut(s) 23, 365, 435
BfaI CTAG 1 cut(s) 353
BglII AGATCT 1 cut(s) 114
BisI GCNGC 1 cut(s) 863
BlsI GCNGC 1 cut(s) 864
Bme1390I CCNGG 3 cut(s) 394, 409, 622
Bme18I GGWCC 1 cut(s) 405
BmgBI CACGTC 1 cut(s) 646
BmgT120I GGNCC 1 cut(s) 405
BmiI GGNNCC 2 cut(s) 534, 670
BmrFI CCNGG 3 cut(s) 394, 409, 622
BpiI GAAGAC 1 cut(s) 506
BplI GAGNNNNNCTC 2 cut(s) 830, 862
Bpu10I CCTNAGC 1 cut(s) 1023
BpuEI CTTGAG 1 cut(s) 131
BsaAI YACGTR 1 cut(s) 65
BsaBI GATNNNNATC 1 cut(s) 828
BsaI GGTCTC 1 cut(s) 435
BsaJI CCNNGG 6 cut(s) 393, 408, 559, 607, 678, 882
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 414, 560, 865
Bse1I ACTGG 3 cut(s) 379, 946, 1047
Bse3DI GCAATG 1 cut(s) 672
Bse8I GATNNNNATC 1 cut(s) 828
BseBI CCWGG 3 cut(s) 394, 409, 622
BseDI CCNNGG 6 cut(s) 393, 408, 559, 607, 678, 882
BseGI GGATG 3 cut(s) 373, 688, 1043
BseJI GATNNNNATC 1 cut(s) 828
BseLI CCNNNNNNNGG 4 cut(s) 217, 414, 560, 865
BseMI GCAATG 1 cut(s) 672
BseMII CTCAG 5 cut(s) 24, 204, 671, 861, 1037
BseNI ACTGG 3 cut(s) 379, 946, 1047
BseRI GAGGAG 3 cut(s) 146, 947, 1028
BseSI GKGCMC 1 cut(s) 162
BseYI CCCAGC 1 cut(s) 858
BshFI GGCC 1 cut(s) 881
BshNI GGYRCC 1 cut(s) 668
BsiHKAI GWGCWC 3 cut(s) 162, 970, 1024
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 4 cut(s) 217, 414, 560, 865
BsmAI GTCTC 3 cut(s) 23, 365, 435
BsmFI GGGAC 1 cut(s) 656
BsnI GGCC 1 cut(s) 881
Bso31I GGTCTC 1 cut(s) 435
Bsp1286I GDGCHC 3 cut(s) 162, 970, 1024
Bsp143I GATC 4 cut(s) 3, 114, 521, 532
Bsp19I CCATGG 1 cut(s) 607
BspACI CCGC 1 cut(s) 862
BspANI GGCC 1 cut(s) 881
BspCNI CTCAG 5 cut(s) 25, 205, 670, 860, 1036
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 2 cut(s) 534, 670
BspPI GGATC 3 cut(s) 527, 529, 540
BspQI GCTCTTC 2 cut(s) 327, 1024
BspT107I GGYRCC 1 cut(s) 668
BspTNI GGTCTC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 672
BsrI ACTGG 3 cut(s) 379, 946, 1047
BssECI CCNNGG 6 cut(s) 393, 408, 559, 607, 678, 882
BssMI GATC 4 cut(s) 3, 114, 521, 532
BssT1I CCWWGG 3 cut(s) 559, 607, 882
Bst2UI CCWGG 3 cut(s) 394, 409, 622
Bst4CI ACNGT 1 cut(s) 754
Bst6I CTCTTC 3 cut(s) 327, 420, 1024
BstAPI GCANNNNNTGC 1 cut(s) 419
BstBAI YACGTR 1 cut(s) 65
BstC8I GCNNGC 5 cut(s) 189, 382, 627, 798, 1064
BstDEI CTNAG 6 cut(s) 33, 127, 213, 657, 847, 1023
BstDSI CCRYGG 1 cut(s) 607
BstF5I GGATG 3 cut(s) 373, 688, 1043
BstKTI GATC 4 cut(s) 6, 117, 524, 535
BstMAI GTCTC 3 cut(s) 23, 365, 435
BstMBI GATC 4 cut(s) 3, 114, 521, 532
BstMWI GCNNNNNNNGC 3 cut(s) 166, 419, 626
BstNI CCWGG 3 cut(s) 394, 409, 622
BstSCI CCNGG 3 cut(s) 392, 407, 620
BstSLI GKGCMC 1 cut(s) 162
BstV2I GAAGAC 1 cut(s) 506
BstX2I RGATCY 2 cut(s) 114, 532
BstXI CCANNNNNNTGG 1 cut(s) 1062
BstYI RGATCY 2 cut(s) 114, 532
BsuRI GGCC 1 cut(s) 881
BtgI CCRYGG 1 cut(s) 607
BtrI CACGTC 1 cut(s) 646
BtsCI GGATG 3 cut(s) 373, 688, 1043
BtsIMutI CAGTG 2 cut(s) 750, 1054
Cac8I GCNNGC 5 cut(s) 189, 382, 627, 798, 1064
CaiI CAGNNNCTG 1 cut(s) 865
CciI TCATGA 1 cut(s) 694
Cfr13I GGNCC 1 cut(s) 405
CspCI CAANNNNNGTGG 2 cut(s) 699, 734
CviAII CATG 7 cut(s) 94, 414, 437, 608, 638, 695, 772
DdeI CTNAG 6 cut(s) 33, 127, 213, 657, 847, 1023
DpnI GATC 4 cut(s) 5, 116, 523, 534
DpnII GATC 4 cut(s) 3, 114, 521, 532
EaeI YGGCCR 1 cut(s) 879
Eam1104I CTCTTC 3 cut(s) 327, 420, 1024
EarI CTCTTC 3 cut(s) 327, 420, 1024
Ecl136II GAGCTC 1 cut(s) 1022
Eco130I CCWWGG 3 cut(s) 559, 607, 882
Eco24I GRGCYC 1 cut(s) 1024
Eco31I GGTCTC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 405
Eco53kI GAGCTC 1 cut(s) 1022
Eco57I CTGAAG 1 cut(s) 444
Eco72I CACGTG 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 1022
EcoRII CCWGG 3 cut(s) 392, 407, 620
EcoT14I CCWWGG 3 cut(s) 559, 607, 882
EcoT38I GRGCYC 1 cut(s) 1024
ErhI CCWWGG 3 cut(s) 559, 607, 882
FaeI CATG 7 cut(s) 97, 417, 440, 611, 641, 698, 775
FalI AAGNNNNNCTT 4 cut(s) 186, 218, 306, 338
FaqI GGGAC 1 cut(s) 656
FatI CATG 7 cut(s) 93, 413, 436, 607, 637, 694, 771
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 863
FokI GGATG 3 cut(s) 380, 695, 1030
FriOI GRGCYC 1 cut(s) 1024
Fsp4HI GCNGC 1 cut(s) 863
FspBI CTAG 1 cut(s) 353
GluI GCNGC 1 cut(s) 863
GsaI CCCAGC 1 cut(s) 862
HaeIII GGCC 1 cut(s) 881
Hin1II CATG 7 cut(s) 97, 417, 440, 611, 641, 698, 775
HincII GTYRAC 1 cut(s) 939
HindII GTYRAC 1 cut(s) 939
HinfI GANTC 4 cut(s) 68, 85, 215, 225
HphI GGTGA 2 cut(s) 379, 965
Hpy166II GTNNAC 2 cut(s) 160, 939
Hpy188I TCNGA 3 cut(s) 220, 660, 682
Hpy8I GTNNAC 2 cut(s) 160, 939
HpyAV CCTTC 3 cut(s) 455, 607, 999
HpyCH4III ACNGT 1 cut(s) 754
HpyCH4IV ACGT 3 cut(s) 64, 645, 691
HpyCH4V TGCA 2 cut(s) 160, 380
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 419, 626
HpyF3I CTNAG 6 cut(s) 33, 127, 213, 657, 847, 1023
HpySE526I ACGT 3 cut(s) 64, 645, 691
Hsp92II CATG 7 cut(s) 97, 417, 440, 611, 641, 698, 775
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 114, 521, 532
LguI GCTCTTC 2 cut(s) 327, 1024
MaeI CTAG 1 cut(s) 353
MaeII ACGT 3 cut(s) 64, 645, 691
MaeIII GTNAC 3 cut(s) 69, 82, 307
MalI GATC 4 cut(s) 5, 116, 523, 534
MboI GATC 4 cut(s) 3, 114, 521, 532
MflI RGATCY 2 cut(s) 114, 532
MhlI GDGCHC 3 cut(s) 162, 970, 1024
MluCI AATT 7 cut(s) 53, 267, 504, 650, 841, 999, 1083
MlyI GAGTC 3 cut(s) 77, 79, 224
MmeI TCCRAC 4 cut(s) 198, 277, 350, 990
MroXI GAANNNNTTC 1 cut(s) 321
MseI TTAA 2 cut(s) 345, 1090
MslI CAYNNNNRTG 2 cut(s) 80, 155
MspA1I CMGCKG 3 cut(s) 851, 862, 1062
MspR9I CCNGG 3 cut(s) 394, 409, 622
MvaI CCWGG 3 cut(s) 394, 409, 622
MwoI GCNNNNNNNGC 3 cut(s) 166, 419, 626
NcoI CCATGG 1 cut(s) 607
NdeII GATC 4 cut(s) 3, 114, 521, 532
NlaIII CATG 7 cut(s) 97, 417, 440, 611, 641, 698, 775
NlaIV GGNNCC 2 cut(s) 534, 670
NmuCI GTSAC 3 cut(s) 69, 82, 307
PagI TCATGA 1 cut(s) 694
PciSI GCTCTTC 2 cut(s) 327, 1024
PdmI GAANNNNTTC 1 cut(s) 321
PfeI GAWTC 1 cut(s) 225
PflMI CCANNNNNTGG 2 cut(s) 414, 865
PkrI GCNGC 1 cut(s) 864
PleI GAGTC 3 cut(s) 76, 79, 223
PmaCI CACGTG 1 cut(s) 65
PmlI CACGTG 1 cut(s) 65
PpsI GAGTC 3 cut(s) 76, 79, 223
Ppu21I YACGTR 1 cut(s) 65
PsiI TTATAA 1 cut(s) 828
Psp124BI GAGCTC 1 cut(s) 1024
Psp6I CCWGG 3 cut(s) 392, 407, 620
PspCI CACGTG 1 cut(s) 65
PspFI CCCAGC 1 cut(s) 858
PspGI CCWGG 3 cut(s) 392, 407, 620
PspN4I GGNNCC 2 cut(s) 534, 670
PspPI GGNCC 1 cut(s) 405
PsrI GAACNNNNNNTAC 2 cut(s) 539, 571
PstNI CAGNNNCTG 1 cut(s) 865
PsuI RGATCY 2 cut(s) 114, 532
PvuII CAGCTG 2 cut(s) 851, 1062
RseI CAYNNNNRTG 2 cut(s) 80, 155
SacI GAGCTC 1 cut(s) 1024
SapI GCTCTTC 2 cut(s) 327, 1024
SaqAI TTAA 2 cut(s) 345, 1090
SatI GCNGC 1 cut(s) 863
Sau3AI GATC 4 cut(s) 3, 114, 521, 532
Sau96I GGNCC 1 cut(s) 405
SchI GAGTC 3 cut(s) 77, 79, 224
ScrFI CCNGG 3 cut(s) 394, 409, 622
SduI GDGCHC 3 cut(s) 162, 970, 1024
SinI GGWCC 1 cut(s) 405
SmiMI CAYNNNNRTG 2 cut(s) 80, 155
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 7 cut(s) 53, 267, 504, 650, 841, 999, 1083
SsiI CCGC 1 cut(s) 862
SspMI CTAG 1 cut(s) 353
SstI GAGCTC 1 cut(s) 1024
StyD4I CCNGG 3 cut(s) 392, 407, 620
StyI CCWWGG 3 cut(s) 559, 607, 882
TaaI ACNGT 1 cut(s) 754
TaiI ACGT 3 cut(s) 67, 648, 694
TaqI TCGA 2 cut(s) 495, 837
TasI AATT 7 cut(s) 53, 267, 504, 650, 841, 999, 1083
TauI GCSGC 1 cut(s) 865
TfiI GAWTC 1 cut(s) 225
Tru1I TTAA 2 cut(s) 345, 1090
Tru9I TTAA 2 cut(s) 345, 1090
TscAI CASTG 2 cut(s) 757, 1054
TseFI GTSAC 3 cut(s) 69, 82, 307
Tsp45I GTSAC 3 cut(s) 69, 82, 307
TspDTI ATGAA 8 cut(s) 56, 500, 556, 567, 589, 699, 711, 791
TspGWI ACGGA 1 cut(s) 557
TspRI CASTG 2 cut(s) 757, 1054
Van91I CCANNNNNTGG 2 cut(s) 414, 865
VneI GTGCAC 1 cut(s) 158
VpaK11BI GGWCC 1 cut(s) 405
XapI RAATTY 2 cut(s) 267, 841
XbaI TCTAGA 1 cut(s) 352
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.