Rw3G000080

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010828
Physical Location & Seq
Reverse (-)
157455 .. 161832
4378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G000080.1

Sequence Viewer

Length: 1203 bp
ATGGCTCTCTGCAGGCTCAACACCTTCCCATCTCACACGCTCATCCCTTGTTCTTCTTCTTCTTCTTCTTCTTCTAATTCCCGCAATTTCAGATCTCCAAAAATCTTCATGGCCTCCACTCGTTATTCCGTCTCCAGAGAGACTGAGAAGATGAAGAAAACTCAATTCCCTCCACGTGAGTCACACATTCAAGTGACTCATTCCATGCCTCCTGAACACCTTGAGATCTTCAAGTCCTTAGAGGAGTGGGCTGATGATAACATCTTGGTGCACCTAAAGCCTGTTGAGAAATGTTGGCAAGCACAAGATTTTCTTCCTGCCCCTGAGTCGGAGGGATTCTATGAGCAAGTAAAGGAGTTGAGGGAAAGAGCAAAAGAAATTCCTGATGATTATTTTGTTGTCTTGGTTGGAGATATGGTCACAGAAGAAGCTCTTCCAACATACCAGACAATGCTTAATACTCTAGATGGAGTTCGGGATGAGACTGGTGCAAGCCTCACCTCCTGGGCAAGATGGACCAGGGCATGGACTGCTGAAGAGAATAGGCATGGAGACCTTCTAAACAAGTATCTCTATCTCTCTGGACGAGTAGATATGAAACAAATCGAGAAGACAATTCAGTATCTTATTGGATCAGGAATGGATCCTAAAACGGAGAACAATCCCTACCTTGGTTTCATTTATACTTCATTTCAAGAGAGGGCTACCTTCATCTCCCATGGGAACACAGCCAGGCAAGCAAAAGAGCATGGGGATGTGAAATTGGCTCAGATATGTGGCACCATTGCCTCGGATGAAAAACGTCATGAAACTGCCTATACAAAGATTGTGGAGAAGCTCTTTGAGATAGACCCAGATGGCACTGTATTGGCTTTAGCTGACATGATGAAGAAAAAAATAACAATGCCTGCCCACTTGATGTATGATGGTCAGGATTATAATCTTTTCGAGAATTTCTCAGCTGTTGCCCAGCGGCTGGAAGTTTATACGGCCAAGGATTACGCTGATATTTTGGAGTTTCTGGTTGGTAGATGGAAAATAGAGGAGTTGACTGGTCTTTCTGGTGAGGGTCGTAGAGCACAAGATTTCGTATGTGGATTGCCACCGAGAATTAGAAGGTTGGAGGAGAGAGCTCAGGGAAGAGCCAAGAAATCATCCAGTGTTCCATTCAGCTGGCTTTTTGGTAGAGAGATAATCGTTTAA

Protein Analysis

400

Amino Acids

45.83

Weight (kDa)

6.0

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 73 - 394 7.6e-160 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02630 AT5G16230 AT5G16240
fragaria_vesca FvH4_6g00080
malus_domestica MD00G1017300.v1.1 MD12G1261600.v1.1
prunus_persica Prupe.6G365100_v2.0.a1 Prupe.6G365100_v2.0.a1 Prupe.6G365200_v2.0.a1
pyrus_communis pycom04g21990
rosa_chinensis RchiOBHm_Chr3g0447411
rosa_laevigata RLG00000026001
rosa_multiflora Rmu_ssc0000473.1_g000020
rosa_roxburghii Rroxscaffold_6G00425610
rosa_rugosa Rorug02G0601300
rosa_samantha Rh3AG001400 Rh3BG001100 Rh3CG001000 Rh3DG001300
rosa_wichuraiana Rw3G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 939
AccB1I GGYRCC 1 cut(s) 779
AccB7I CCANNNNNTGG 2 cut(s) 525, 976
AciI CCGC 2 cut(s) 82, 973
AclWI GGATC 3 cut(s) 638, 640, 651
AcoI YGGCCR 1 cut(s) 990
AcsI RAATTY 2 cut(s) 378, 952
AcuI CTGAAG 1 cut(s) 555
AcvI CACGTG 1 cut(s) 176
AfiI CCNNNNNNNGG 4 cut(s) 328, 525, 671, 976
AgsI TTSAA 3 cut(s) 191, 232, 695
AjnI CCWGG 3 cut(s) 503, 518, 731
AluBI AGCT 6 cut(s) 431, 838, 878, 962, 1133, 1173
AluI AGCT 6 cut(s) 431, 838, 878, 962, 1133, 1173
Alw21I GWGCWC 3 cut(s) 273, 1081, 1135
Alw26I GTCTC 4 cut(s) 134, 136, 476, 546
Alw44I GTGCAC 1 cut(s) 269
AlwI GGATC 3 cut(s) 638, 640, 651
AlwNI CAGNNNCTG 1 cut(s) 976
AoxI GGCC 2 cut(s) 111, 990
ApaLI GTGCAC 1 cut(s) 269
ApoI RAATTY 2 cut(s) 378, 952
Asp700I GAANNNNTTC 1 cut(s) 432
AspS9I GGNCC 1 cut(s) 516
AsuHPI GGTGA 2 cut(s) 490, 1076
AvaII GGWCC 1 cut(s) 516
BaeGI GKGCMC 1 cut(s) 273
BamHI GGATCC 1 cut(s) 643
BanI GGYRCC 1 cut(s) 779
BanII GRGCYC 1 cut(s) 1135
BbrPI CACGTG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 617
Bbv12I GWGCWC 3 cut(s) 273, 1081, 1135
BccI CCATC 6 cut(s) 37, 461, 507, 851, 920, 1026
BceAI ACGGC 1 cut(s) 1005
BciT130I CCWGG 3 cut(s) 505, 520, 733
BcoDI GTCTC 4 cut(s) 134, 136, 476, 546
BfaI CTAG 1 cut(s) 464
BfmI CTRYAG 1 cut(s) 10
BglII AGATCT 2 cut(s) 92, 225
BisI GCNGC 1 cut(s) 974
BlsI GCNGC 1 cut(s) 975
Bme1390I CCNGG 3 cut(s) 505, 520, 733
Bme18I GGWCC 1 cut(s) 516
BmgT120I GGNCC 1 cut(s) 516
BmiI GGNNCC 2 cut(s) 645, 781
BmrFI CCNGG 3 cut(s) 505, 520, 733
BpiI GAAGAC 1 cut(s) 617
BplI GAGNNNNNCTC 2 cut(s) 941, 973
BpmI CTGGAG 1 cut(s) 118
Bpu10I CCTNAGC 1 cut(s) 1134
BpuEI CTTGAG 1 cut(s) 242
BsaAI YACGTR 1 cut(s) 176
BsaBI GATNNNNATC 1 cut(s) 939
BsaI GGTCTC 1 cut(s) 546
BsaJI CCNNGG 6 cut(s) 504, 519, 670, 718, 789, 993
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bsc4I CCNNNNNNNGG 4 cut(s) 328, 525, 671, 976
Bse1I ACTGG 3 cut(s) 490, 1057, 1158
Bse3DI GCAATG 1 cut(s) 783
Bse8I GATNNNNATC 1 cut(s) 939
BseBI CCWGG 3 cut(s) 505, 520, 733
BseDI CCNNGG 6 cut(s) 504, 519, 670, 718, 789, 993
BseGI GGATG 5 cut(s) 42, 484, 760, 799, 1154
BseJI GATNNNNATC 1 cut(s) 939
BseLI CCNNNNNNNGG 4 cut(s) 328, 525, 671, 976
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 5 cut(s) 135, 315, 782, 972, 1148
BseNI ACTGG 3 cut(s) 490, 1057, 1158
BseRI GAGGAG 3 cut(s) 257, 1058, 1139
BseSI GKGCMC 1 cut(s) 273
BseYI CCCAGC 1 cut(s) 969
BshFI GGCC 2 cut(s) 113, 992
BshNI GGYRCC 1 cut(s) 779
BsiHKAI GWGCWC 3 cut(s) 273, 1081, 1135
BslI CCNNNNNNNGG 4 cut(s) 328, 525, 671, 976
BsmAI GTCTC 4 cut(s) 134, 136, 476, 546
BsmBI CGTCTC 1 cut(s) 136
BsnI GGCC 2 cut(s) 113, 992
Bso31I GGTCTC 1 cut(s) 546
Bsp1286I GDGCHC 3 cut(s) 273, 1081, 1135
Bsp143I GATC 4 cut(s) 92, 225, 632, 643
Bsp19I CCATGG 1 cut(s) 718
BspACI CCGC 2 cut(s) 82, 973
BspANI GGCC 2 cut(s) 113, 992
BspCNI CTCAG 5 cut(s) 136, 316, 781, 971, 1147
BspHI TCATGA 1 cut(s) 805
BspLI GGNNCC 2 cut(s) 645, 781
BspMAI CTGCAG 1 cut(s) 14
BspPI GGATC 3 cut(s) 638, 640, 651
BspQI GCTCTTC 2 cut(s) 438, 1135
BspT107I GGYRCC 1 cut(s) 779
BspTNI GGTCTC 1 cut(s) 546
BsrDI GCAATG 1 cut(s) 783
BsrI ACTGG 3 cut(s) 490, 1057, 1158
BssECI CCNNGG 6 cut(s) 504, 519, 670, 718, 789, 993
BssMI GATC 4 cut(s) 92, 225, 632, 643
BssT1I CCWWGG 3 cut(s) 670, 718, 993
Bst2UI CCWGG 3 cut(s) 505, 520, 733
Bst4CI ACNGT 1 cut(s) 865
Bst6I CTCTTC 3 cut(s) 438, 531, 1135
BstAPI GCANNNNNTGC 1 cut(s) 530
BstBAI YACGTR 1 cut(s) 176
BstC8I GCNNGC 6 cut(s) 14, 300, 493, 738, 909, 1175
BstDEI CTNAG 6 cut(s) 144, 238, 324, 768, 958, 1134
BstDSI CCRYGG 1 cut(s) 718
BstF5I GGATG 5 cut(s) 42, 484, 760, 799, 1154
BstKTI GATC 4 cut(s) 95, 228, 635, 646
BstMAI GTCTC 4 cut(s) 134, 136, 476, 546
BstMBI GATC 4 cut(s) 92, 225, 632, 643
BstMWI GCNNNNNNNGC 3 cut(s) 277, 530, 737
BstNI CCWGG 3 cut(s) 505, 520, 733
BstSCI CCNGG 3 cut(s) 503, 518, 731
BstSFI CTRYAG 1 cut(s) 10
BstSLI GKGCMC 1 cut(s) 273
BstV2I GAAGAC 1 cut(s) 617
BstX2I RGATCY 3 cut(s) 92, 225, 643
BstXI CCANNNNNNTGG 1 cut(s) 1173
BstYI RGATCY 3 cut(s) 92, 225, 643
BsuRI GGCC 2 cut(s) 113, 992
BtgI CCRYGG 1 cut(s) 718
BtsCI GGATG 5 cut(s) 42, 484, 760, 799, 1154
BtsIMutI CAGTG 2 cut(s) 861, 1165
Cac8I GCNNGC 6 cut(s) 14, 300, 493, 738, 909, 1175
CaiI CAGNNNCTG 1 cut(s) 976
CciI TCATGA 1 cut(s) 805
Cfr13I GGNCC 1 cut(s) 516
CspCI CAANNNNNGTGG 2 cut(s) 810, 845
CviAII CATG 8 cut(s) 109, 205, 525, 548, 719, 749, 806, 883
DdeI CTNAG 6 cut(s) 144, 238, 324, 768, 958, 1134
DpnI GATC 4 cut(s) 94, 227, 634, 645
DpnII GATC 4 cut(s) 92, 225, 632, 643
EaeI YGGCCR 1 cut(s) 990
Eam1104I CTCTTC 3 cut(s) 438, 531, 1135
EarI CTCTTC 3 cut(s) 438, 531, 1135
Ecl136II GAGCTC 1 cut(s) 1133
Eco130I CCWWGG 3 cut(s) 670, 718, 993
Eco24I GRGCYC 1 cut(s) 1135
Eco31I GGTCTC 1 cut(s) 546
Eco47I GGWCC 1 cut(s) 516
Eco53kI GAGCTC 1 cut(s) 1133
Eco57I CTGAAG 1 cut(s) 555
Eco72I CACGTG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 1133
EcoRII CCWGG 3 cut(s) 503, 518, 731
EcoT14I CCWWGG 3 cut(s) 670, 718, 993
EcoT38I GRGCYC 1 cut(s) 1135
ErhI CCWWGG 3 cut(s) 670, 718, 993
Esp3I CGTCTC 1 cut(s) 136
FaeI CATG 8 cut(s) 112, 208, 528, 551, 722, 752, 809, 886
FalI AAGNNNNNCTT 4 cut(s) 297, 329, 417, 449
FatI CATG 8 cut(s) 108, 204, 524, 547, 718, 748, 805, 882
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 1 cut(s) 974
FokI GGATG 5 cut(s) 29, 491, 767, 806, 1141
FriOI GRGCYC 1 cut(s) 1135
Fsp4HI GCNGC 1 cut(s) 974
FspBI CTAG 1 cut(s) 464
GluI GCNGC 1 cut(s) 974
GsaI CCCAGC 1 cut(s) 973
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 2 cut(s) 113, 992
Hin1II CATG 8 cut(s) 112, 208, 528, 551, 722, 752, 809, 886
HincII GTYRAC 1 cut(s) 1050
HindII GTYRAC 1 cut(s) 1050
HinfI GANTC 4 cut(s) 179, 196, 326, 336
HphI GGTGA 2 cut(s) 490, 1076
Hpy166II GTNNAC 2 cut(s) 271, 1050
Hpy188I TCNGA 4 cut(s) 92, 331, 771, 793
Hpy8I GTNNAC 2 cut(s) 271, 1050
HpyAV CCTTC 4 cut(s) 34, 566, 718, 1110
HpyCH4III ACNGT 1 cut(s) 865
HpyCH4IV ACGT 2 cut(s) 175, 802
HpyCH4V TGCA 3 cut(s) 12, 271, 491
HpyF10VI GCNNNNNNNGC 3 cut(s) 277, 530, 737
HpyF3I CTNAG 6 cut(s) 144, 238, 324, 768, 958, 1134
HpySE526I ACGT 2 cut(s) 175, 802
Hsp92II CATG 8 cut(s) 112, 208, 528, 551, 722, 752, 809, 886
Kzo9I GATC 4 cut(s) 92, 225, 632, 643
LguI GCTCTTC 2 cut(s) 438, 1135
MaeI CTAG 1 cut(s) 464
MaeII ACGT 2 cut(s) 175, 802
MaeIII GTNAC 3 cut(s) 180, 193, 418
MalI GATC 4 cut(s) 94, 227, 634, 645
MboI GATC 4 cut(s) 92, 225, 632, 643
MflI RGATCY 3 cut(s) 92, 225, 643
MhlI GDGCHC 3 cut(s) 273, 1081, 1135
MluCI AATT 8 cut(s) 76, 85, 164, 378, 615, 761, 952, 1110
MlyI GAGTC 3 cut(s) 188, 190, 335
MmeI TCCRAC 4 cut(s) 309, 388, 461, 1101
MroXI GAANNNNTTC 1 cut(s) 432
MseI TTAA 2 cut(s) 456, 1201
MslI CAYNNNNRTG 3 cut(s) 191, 266, 753
MspA1I CMGCKG 3 cut(s) 962, 973, 1173
MspR9I CCNGG 3 cut(s) 505, 520, 733
MvaI CCWGG 3 cut(s) 505, 520, 733
MwoI GCNNNNNNNGC 3 cut(s) 277, 530, 737
NcoI CCATGG 1 cut(s) 718
NdeII GATC 4 cut(s) 92, 225, 632, 643
NlaIII CATG 8 cut(s) 112, 208, 528, 551, 722, 752, 809, 886
NlaIV GGNNCC 2 cut(s) 645, 781
NmuCI GTSAC 3 cut(s) 180, 193, 418
PagI TCATGA 1 cut(s) 805
PciSI GCTCTTC 2 cut(s) 438, 1135
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 1 cut(s) 336
PflMI CCANNNNNTGG 2 cut(s) 525, 976
PkrI GCNGC 1 cut(s) 975
PleI GAGTC 3 cut(s) 187, 190, 334
PmaCI CACGTG 1 cut(s) 176
PmlI CACGTG 1 cut(s) 176
PpsI GAGTC 3 cut(s) 187, 190, 334
Ppu21I YACGTR 1 cut(s) 176
PsiI TTATAA 1 cut(s) 939
Psp124BI GAGCTC 1 cut(s) 1135
Psp6I CCWGG 3 cut(s) 503, 518, 731
PspCI CACGTG 1 cut(s) 176
PspFI CCCAGC 1 cut(s) 969
PspGI CCWGG 3 cut(s) 503, 518, 731
PspN4I GGNNCC 2 cut(s) 645, 781
PspPI GGNCC 1 cut(s) 516
PsrI GAACNNNNNNTAC 2 cut(s) 650, 682
PstI CTGCAG 1 cut(s) 14
PstNI CAGNNNCTG 1 cut(s) 976
PsuI RGATCY 3 cut(s) 92, 225, 643
PvuII CAGCTG 2 cut(s) 962, 1173
RseI CAYNNNNRTG 3 cut(s) 191, 266, 753
SacI GAGCTC 1 cut(s) 1135
SapI GCTCTTC 2 cut(s) 438, 1135
SaqAI TTAA 2 cut(s) 456, 1201
SatI GCNGC 1 cut(s) 974
Sau3AI GATC 4 cut(s) 92, 225, 632, 643
Sau96I GGNCC 1 cut(s) 516
SchI GAGTC 3 cut(s) 188, 190, 335
ScrFI CCNGG 3 cut(s) 505, 520, 733
SduI GDGCHC 3 cut(s) 273, 1081, 1135
SfcI CTRYAG 1 cut(s) 10
SinI GGWCC 1 cut(s) 516
SmiMI CAYNNNNRTG 3 cut(s) 191, 266, 753
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 8 cut(s) 76, 85, 164, 378, 615, 761, 952, 1110
SsiI CCGC 2 cut(s) 82, 973
SspMI CTAG 1 cut(s) 464
SstI GAGCTC 1 cut(s) 1135
StyD4I CCNGG 3 cut(s) 503, 518, 731
StyI CCWWGG 3 cut(s) 670, 718, 993
TaaI ACNGT 1 cut(s) 865
TaiI ACGT 2 cut(s) 178, 805
TaqI TCGA 2 cut(s) 606, 948
TasI AATT 8 cut(s) 76, 85, 164, 378, 615, 761, 952, 1110
TauI GCSGC 1 cut(s) 976
TfiI GAWTC 1 cut(s) 336
Tru1I TTAA 2 cut(s) 456, 1201
Tru9I TTAA 2 cut(s) 456, 1201
TscAI CASTG 2 cut(s) 868, 1165
TseFI GTSAC 3 cut(s) 180, 193, 418
Tsp45I GTSAC 3 cut(s) 180, 193, 418
TspDTI ATGAA 9 cut(s) 97, 167, 611, 667, 678, 700, 810, 822, 902
TspGWI ACGGA 2 cut(s) 118, 668
TspRI CASTG 2 cut(s) 868, 1165
Van91I CCANNNNNTGG 2 cut(s) 525, 976
VneI GTGCAC 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 516
XapI RAATTY 2 cut(s) 378, 952
XbaI TCTAGA 1 cut(s) 463
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.