AT5G16240

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010828
Physical Location & Seq
Forward (+)
5306740 .. 5309961
3222 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16240.1

Sequence Viewer

Length: 1185 bp
ATGGTTATGGCTATGGATCGGATCGCTTTGTTCTCTTCCTCATCATCTGTTTATCATCACGGTTCTTCTCATTCTCATGGCTCCAAATCTTCCAGAGTTTTCACTATTCGTTCCGACTCTACAGCGGTTGGAAGGAAACTGTATATTCCTCCTCGAGAGGTTCATCTTCAAGTGAAGTATTCAATGCCACCACAAAAGCTGGAGATTTTCAAGTCCTTAGAAGGATGGGCTAATGACAACTTGTTGGCTTACTTAAAACCTGTTGAGAAATCATGGCAACCAACTGATTTTCTCCCTGAACCTGAGTCAGAAGGATTCTATGACCAAGTTAAGGAGTTAAGAGAAAGGTGTAAGGAGCTCTCTGATGACTACCTTATAGTGCTTGTTGGTGATATGATCACAGAGGAAGCACTTCCGACTTATCAGACCATGATTAATACATTGGACGGGGTTAGAGATGAGACGGGAGCAAGTCCAACTCCTTGGGCAGTATGGACTAGGGCATGGACTGCGGAAGAGAATAGACATGGTGATCTTCTTAACAAGTACCTTTATCTGTCTGGACGAGTAGACATGAGACAGATTGAAAAAACTATTCAATACCTCATTGGTTCTGGAATGGATCCAAAAACTGAAAACAATCCTTACTTGGGATTCATATATACTTCTTTTCAAGAAAGAGCTACATTCATCTCCCATGGAAACACTGCTAGACTGGCAAAGGATCTTGGAGACTTGACACTTGGGAAGATTTGTGGCACCATTGCTGCTGATGAGAGGCGCCATGAACACGCCTACACCAAGATTGTAGAGAAGCTCTTTGAAATTGATCCTGATACCACAGTCGTGGGCTTTGCTGACATGATGAGGAAAAAGATCTCGATGCCTGCTCATTTGATGTATGATGGTCGTGATGATAATCTATTTGATCACTTCTCGTCCGTGGCCCAGAGGCTTGGTGTCTACACTGCCAAGGACTATGCTGATATACTGCAACATCTTGTGGAAAGGTGGAATGTGGAGAAGTTGTCAGACCTTTCTAGTGAAGGAAACAGGGCCCAGGACTACCTCTGTGGATTACCTGCTAGAATCCGCAAACTTGAAGAGAGGGCTCAAGGGAGAACCAAAGAAGCAGCAAAAAACATACCATTCAGCTGGATTTTTGGTCGAGAAGTCAGGGCTTAA

Protein Analysis

394

Amino Acids

45.02

Weight (kDa)

6.09

Isoelectric Point (pI)

34.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 65 - 388 7.7e-160 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02630 AT5G16230 AT5G16240
fragaria_vesca FvH4_6g00080
malus_domestica MD00G1017300.v1.1 MD12G1261600.v1.1
prunus_persica Prupe.6G365100_v2.0.a1 Prupe.6G365100_v2.0.a1 Prupe.6G365200_v2.0.a1
pyrus_communis pycom04g21990
rosa_chinensis RchiOBHm_Chr3g0447411
rosa_laevigata RLG00000026001
rosa_multiflora Rmu_ssc0000473.1_g000020
rosa_roxburghii Rroxscaffold_6G00425610
rosa_rugosa Rorug02G0601300
rosa_samantha Rh3AG001400 Rh3BG001100 Rh3CG001000 Rh3DG001300
rosa_wichuraiana Rw3G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1090
AccB1I GGYRCC 2 cut(s) 758, 780
AccI GTMKAC 2 cut(s) 570, 963
AciI CCGC 3 cut(s) 125, 512, 1093
AclWI GGATC 6 cut(s) 24, 29, 617, 630, 732, 824
AcyI GRCGYC 1 cut(s) 781
AfaI GTAC 1 cut(s) 548
AfiI CCNNNNNNNGG 2 cut(s) 331, 650
AgsI TTSAA 8 cut(s) 170, 183, 211, 587, 599, 674, 824, 1103
AjnI CCWGG 1 cut(s) 1059
AleI CACNNNNGTG 1 cut(s) 845
AluBI AGCT 5 cut(s) 199, 358, 683, 817, 1155
AluI AGCT 5 cut(s) 199, 358, 683, 817, 1155
Alw21I GWGCWC 1 cut(s) 360
Alw26I GTCTC 3 cut(s) 455, 571, 726
AlwI GGATC 6 cut(s) 24, 29, 617, 630, 732, 824
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 2 cut(s) 945, 1056
ApaI GGGCCC 1 cut(s) 1060
ApeKI GCWGC 2 cut(s) 767, 1133
AseI ATTAAT 1 cut(s) 435
Asp700I GAANNNNTTC 1 cut(s) 411
AspLEI GCGC 1 cut(s) 783
AspS9I GGNCC 3 cut(s) 946, 1056, 1057
AsuHPI GGTGA 2 cut(s) 401, 542
AvaI CYCGRG 1 cut(s) 153
BaeGI GKGCMC 1 cut(s) 1060
BaeI ACNNNNGTAYC 2 cut(s) 828, 861
BamHI GGATCC 1 cut(s) 622
BanI GGYRCC 2 cut(s) 758, 780
BanII GRGCYC 3 cut(s) 360, 1060, 1114
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 2 cut(s) 754, 1145
BccI CCATC 2 cut(s) 219, 899
BciT130I CCWGG 1 cut(s) 1061
BclI TGATCA 2 cut(s) 396, 928
BcoDI GTCTC 3 cut(s) 455, 571, 726
BfaI CTAG 4 cut(s) 498, 711, 1041, 1086
BfmI CTRYAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 784
BfuAI ACCTGC 1 cut(s) 1090
BglII AGATCT 1 cut(s) 876
BisI GCNGC 2 cut(s) 768, 1134
BlsI GCNGC 2 cut(s) 769, 1135
Bme1390I CCNGG 1 cut(s) 1061
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 946, 1056, 1057
BmiI GGNNCC 5 cut(s) 82, 624, 760, 782, 1058
BmrFI CCNGG 1 cut(s) 1061
BmsI GCATC 1 cut(s) 873
BpmI CTGGAG 1 cut(s) 221
BpuEI CTTGAG 1 cut(s) 1098
BsaBI GATNNNNATC 1 cut(s) 918
BsaHI GRCGYC 1 cut(s) 781
BsaJI CCNNGG 5 cut(s) 482, 697, 942, 972, 1059
BsaXI ACNNNNNCTCC 2 cut(s) 1013, 1043
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 650
Bse1I ACTGG 1 cut(s) 720
Bse3DI GCAATG 1 cut(s) 762
Bse8I GATNNNNATC 1 cut(s) 918
BseBI CCWGG 1 cut(s) 1061
BseDI CCNNGG 5 cut(s) 482, 697, 942, 972, 1059
BseGI GGATG 1 cut(s) 230
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 2 cut(s) 331, 650
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 720
BseRI GAGGAG 1 cut(s) 141
BseSI GKGCMC 1 cut(s) 1060
BseXI GCAGC 2 cut(s) 754, 1145
BshFI GGCC 2 cut(s) 947, 1058
BshNI GGYRCC 2 cut(s) 758, 780
BsiHKAI GWGCWC 1 cut(s) 360
BsiHKCI CYCGRG 1 cut(s) 153
BslI CCNNNNNNNGG 2 cut(s) 331, 650
BsmAI GTCTC 3 cut(s) 455, 571, 726
BsmBI CGTCTC 1 cut(s) 455
BsnI GGCC 2 cut(s) 947, 1058
BsoBI CYCGRG 1 cut(s) 153
Bsp120I GGGCCC 1 cut(s) 1056
Bsp1286I GDGCHC 3 cut(s) 360, 1060, 1114
Bsp143I GATC 9 cut(s) 16, 21, 396, 532, 622, 724, 829, 876, 928
Bsp19I CCATGG 1 cut(s) 697
BspACI CCGC 3 cut(s) 125, 512, 1093
BspANI GGCC 2 cut(s) 947, 1058
BspCNI CTCAG 1 cut(s) 295
BspLI GGNNCC 5 cut(s) 82, 624, 760, 782, 1058
BspMI ACCTGC 1 cut(s) 1090
BspPI GGATC 6 cut(s) 24, 29, 617, 630, 732, 824
BspT107I GGYRCC 2 cut(s) 758, 780
BsrDI GCAATG 1 cut(s) 762
BsrI ACTGG 1 cut(s) 720
BssECI CCNNGG 5 cut(s) 482, 697, 942, 972, 1059
BssMI GATC 9 cut(s) 16, 21, 396, 532, 622, 724, 829, 876, 928
BssNI GRCGYC 1 cut(s) 781
BssT1I CCWWGG 3 cut(s) 482, 697, 972
Bst2UI CCWGG 1 cut(s) 1061
Bst4CI ACNGT 3 cut(s) 62, 141, 844
Bst6I CTCTTC 3 cut(s) 40, 510, 1098
BstACI GRCGYC 1 cut(s) 781
BstAPI GCANNNNNTGC 1 cut(s) 509
BstC8I GCNNGC 1 cut(s) 888
BstDEI CTNAG 2 cut(s) 217, 303
BstDSI CCRYGG 2 cut(s) 697, 942
BstF5I GGATG 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 784
BstHHI GCGC 1 cut(s) 783
BstKTI GATC 9 cut(s) 19, 24, 399, 535, 625, 727, 832, 879, 931
BstMAI GTCTC 3 cut(s) 455, 571, 726
BstMBI GATC 9 cut(s) 16, 21, 396, 532, 622, 724, 829, 876, 928
BstMWI GCNNNNNNNGC 2 cut(s) 509, 716
BstNI CCWGG 1 cut(s) 1061
BstSCI CCNGG 1 cut(s) 1059
BstSFI CTRYAG 1 cut(s) 120
BstSLI GKGCMC 1 cut(s) 1060
BstV1I GCAGC 2 cut(s) 754, 1145
BstX2I RGATCY 3 cut(s) 622, 724, 876
BstXI CCANNNNNNTGG 4 cut(s) 483, 847, 956, 1155
BstYI RGATCY 3 cut(s) 622, 724, 876
BsuRI GGCC 2 cut(s) 947, 1058
BtgI CCRYGG 2 cut(s) 697, 942
BtsCI GGATG 1 cut(s) 230
BtsI GCAGTG 2 cut(s) 705, 966
BtsIMutI CAGTG 2 cut(s) 705, 966
BveI ACCTGC 1 cut(s) 1090
Cac8I GCNNGC 1 cut(s) 888
CfoI GCGC 1 cut(s) 783
Cfr13I GGNCC 3 cut(s) 946, 1056, 1057
Csp6I GTAC 1 cut(s) 547
CviAII CATG 9 cut(s) 77, 273, 430, 504, 527, 574, 698, 785, 862
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 2 cut(s) 217, 303
DinI GGCGCC 1 cut(s) 782
DpnI GATC 9 cut(s) 18, 23, 398, 534, 624, 726, 831, 878, 930
DpnII GATC 9 cut(s) 16, 21, 396, 532, 622, 724, 829, 876, 928
Eam1104I CTCTTC 3 cut(s) 40, 510, 1098
EarI CTCTTC 3 cut(s) 40, 510, 1098
Ecl136II GAGCTC 1 cut(s) 358
Eco130I CCWWGG 3 cut(s) 482, 697, 972
Eco24I GRGCYC 3 cut(s) 360, 1060, 1114
Eco53kI GAGCTC 1 cut(s) 358
Eco88I CYCGRG 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 358
EcoO109I RGGNCCY 1 cut(s) 1056
EcoRII CCWGG 1 cut(s) 1059
EcoT14I CCWWGG 3 cut(s) 482, 697, 972
EcoT38I GRGCYC 3 cut(s) 360, 1060, 1114
EgeI GGCGCC 1 cut(s) 782
EheI GGCGCC 1 cut(s) 782
ErhI CCWWGG 3 cut(s) 482, 697, 972
Esp3I CGTCTC 1 cut(s) 455
FaeI CATG 9 cut(s) 80, 276, 433, 507, 530, 577, 701, 788, 865
FatI CATG 9 cut(s) 76, 272, 429, 503, 526, 573, 697, 784, 861
FbaI TGATCA 2 cut(s) 396, 928
FblI GTMKAC 2 cut(s) 570, 963
Fnu4HI GCNGC 2 cut(s) 768, 1134
FokI GGATG 1 cut(s) 237
FriOI GRGCYC 3 cut(s) 360, 1060, 1114
Fsp4HI GCNGC 2 cut(s) 768, 1134
FspBI CTAG 4 cut(s) 498, 711, 1041, 1086
GlaI GCGC 1 cut(s) 782
GluI GCNGC 2 cut(s) 768, 1134
GsuI CTGGAG 1 cut(s) 221
HaeII RGCGCY 1 cut(s) 784
HaeIII GGCC 2 cut(s) 947, 1058
HhaI GCGC 1 cut(s) 783
Hin1I GRCGYC 1 cut(s) 781
Hin1II CATG 9 cut(s) 80, 276, 433, 507, 530, 577, 701, 788, 865
Hin6I GCGC 1 cut(s) 781
HinP1I GCGC 1 cut(s) 781
HinfI GANTC 5 cut(s) 116, 305, 315, 654, 1089
HphI GGTGA 2 cut(s) 401, 542
Hpy166II GTNNAC 2 cut(s) 571, 964
Hpy188I TCNGA 7 cut(s) 21, 115, 310, 364, 417, 426, 1033
Hpy188III TCNNGA 9 cut(s) 93, 155, 561, 615, 674, 833, 880, 911, 1169
Hpy8I GTNNAC 2 cut(s) 571, 964
HpyAV CCTTC 4 cut(s) 126, 215, 305, 1040
HpyCH4III ACNGT 3 cut(s) 62, 141, 844
HpyCH4V TGCA 1 cut(s) 994
HpyF10VI GCNNNNNNNGC 2 cut(s) 509, 716
HpyF3I CTNAG 2 cut(s) 217, 303
Hsp92I GRCGYC 1 cut(s) 781
Hsp92II CATG 9 cut(s) 80, 276, 433, 507, 530, 577, 701, 788, 865
HspAI GCGC 1 cut(s) 781
KasI GGCGCC 1 cut(s) 780
Ksp22I TGATCA 2 cut(s) 396, 928
Kzo9I GATC 9 cut(s) 16, 21, 396, 532, 622, 724, 829, 876, 928
LmnI GCTCC 3 cut(s) 86, 355, 467
Lsp1109I GCAGC 2 cut(s) 754, 1145
LweI GCATC 1 cut(s) 873
MaeI CTAG 4 cut(s) 498, 711, 1041, 1086
MalI GATC 9 cut(s) 18, 23, 398, 534, 624, 726, 831, 878, 930
MboI GATC 9 cut(s) 16, 21, 396, 532, 622, 724, 829, 876, 928
MboII GAAGA 8 cut(s) 27, 57, 81, 158, 527, 527, 760, 1115
MflI RGATCY 3 cut(s) 622, 724, 876
MhlI GDGCHC 3 cut(s) 360, 1060, 1114
MluCI AATT 1 cut(s) 825
Mly113I GGCGCC 1 cut(s) 781
MlyI GAGTC 2 cut(s) 110, 314
MmeI TCCRAC 4 cut(s) 109, 138, 440, 500
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 6 cut(s) 254, 330, 338, 435, 540, 1183
MslI CAYNNNNRTG 2 cut(s) 75, 845
MspA1I CMGCKG 2 cut(s) 125, 1155
MspR9I CCNGG 1 cut(s) 1061
MvaI CCWGG 1 cut(s) 1061
MwoI GCNNNNNNNGC 2 cut(s) 509, 716
NarI GGCGCC 1 cut(s) 781
NcoI CCATGG 1 cut(s) 697
NdeII GATC 9 cut(s) 16, 21, 396, 532, 622, 724, 829, 876, 928
NlaIII CATG 9 cut(s) 80, 276, 433, 507, 530, 577, 701, 788, 865
NlaIV GGNNCC 5 cut(s) 82, 624, 760, 782, 1058
OliI CACNNNNGTG 1 cut(s) 845
PaeR7I CTCGAG 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 3 cut(s) 315, 654, 1089
PkrI GCNGC 2 cut(s) 769, 1135
PleI GAGTC 2 cut(s) 110, 313
PluTI GGCGCC 1 cut(s) 784
PpsI GAGTC 2 cut(s) 110, 313
PshBI ATTAAT 1 cut(s) 435
Psp124BI GAGCTC 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 1059
PspGI CCWGG 1 cut(s) 1059
PspN4I GGNNCC 5 cut(s) 82, 624, 760, 782, 1058
PspOMI GGGCCC 1 cut(s) 1056
PspPI GGNCC 3 cut(s) 946, 1056, 1057
PsuI RGATCY 3 cut(s) 622, 724, 876
PvuII CAGCTG 1 cut(s) 1155
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
RseI CAYNNNNRTG 2 cut(s) 75, 845
SacI GAGCTC 1 cut(s) 360
SaqAI TTAA 6 cut(s) 254, 330, 338, 435, 540, 1183
SatI GCNGC 2 cut(s) 768, 1134
Sau3AI GATC 9 cut(s) 16, 21, 396, 532, 622, 724, 829, 876, 928
Sau96I GGNCC 3 cut(s) 946, 1056, 1057
SchI GAGTC 2 cut(s) 110, 314
ScrFI CCNGG 1 cut(s) 1061
SduI GDGCHC 3 cut(s) 360, 1060, 1114
SfaNI GCATC 1 cut(s) 873
SfcI CTRYAG 1 cut(s) 120
SfoI GGCGCC 1 cut(s) 782
Sfr274I CTCGAG 1 cut(s) 153
SlaI CTCGAG 1 cut(s) 153
SmiMI CAYNNNNRTG 2 cut(s) 75, 845
SmlI CTYRAG 2 cut(s) 153, 1113
SmoI CTYRAG 2 cut(s) 153, 1113
Sse9I AATT 1 cut(s) 825
SsiI CCGC 3 cut(s) 125, 512, 1093
SspDI GGCGCC 1 cut(s) 780
SspMI CTAG 4 cut(s) 498, 711, 1041, 1086
SstI GAGCTC 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 1059
StyI CCWWGG 3 cut(s) 482, 697, 972
TaaI ACNGT 3 cut(s) 62, 141, 844
TaqI TCGA 3 cut(s) 154, 881, 1168
TasI AATT 1 cut(s) 825
TfiI GAWTC 3 cut(s) 315, 654, 1089
Tru1I TTAA 6 cut(s) 254, 330, 338, 435, 540, 1183
Tru9I TTAA 6 cut(s) 254, 330, 338, 435, 540, 1183
TscAI CASTG 2 cut(s) 712, 973
TseI GCWGC 2 cut(s) 767, 1133
TspDTI ATGAA 4 cut(s) 152, 646, 679, 801
TspGWI ACGGA 1 cut(s) 931
TspRI CASTG 2 cut(s) 712, 973
VspI ATTAAT 1 cut(s) 435
XhoI CTCGAG 1 cut(s) 153
XmiI GTMKAC 2 cut(s) 570, 963
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 4 cut(s) 498, 711, 1041, 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.