AT3G02650

Pentatricopeptide repeat-containing protein At3g02650

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011325
Physical Location & Seq
Forward (+)
568012 .. 570103
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G02650.1

Sequence Viewer

Length: 1731 bp
ATGTTGAGATCCTTTCTCTGTAGATCGCAAAATGCGTCTCGGAATCTCGCTGTTACTCGAATCTCGAAGAAGAAGACCCAGACGACTCATTCGCTTACCTCTCTTTCGAGATTTTCATATCTCGAGTCGTCTGGAAATGCTTCTGTTCGAAACATCAGGTTCTTCTCCACGAGCCCTCCTACGGAAGAGAACCCAGTCTCGTTACCTGCCGATGAGATCCCAATCTCGTCAGCCGCAGAGCTTACCCTTGAAGAATCGGTTGCTTCCGCTTTGGGATTTTCAGAAAGTGGCGATTATGGTGGTACTTCCGTGGAAGCTGTTGGTGAAGATGGAGATTCAGAGATTGTTGCAATAGAAAACGAGGTTTACCAATTTGATGACGAAAAGCTGGAATCTGTACTGTCTTTGTTACGGAGTGATGAAGAATCCTTGGAGTTTGGTCTTAATGCGCTTAATGTCGATTTACACTTAGATTTTGTGGTAAGAGTGTTTGAATCCCCAGGAATTTCAGGTAAGAATTTGATTAGGTTTTTGAAGTGGGCAACTCAGAATGAAGAGATTACTGTTACCACCTCGCTTGTAGAATCTCTTCTTGTAGCAATTGCTAGTGATACACGTAGAATGGATGCTTATGGTTTGTGGGATTTGGTTAAGGAGATAGGTGAGAAAGAGAGTTGTGGTGTTTTGAATTTGGAGATATTGAATGAACTGATTGCCTTGTTTGGGAAACTTGGTAAATCCAAAGCTGCTTTTGATGTGTTTAGTAAGACCGAAGAGTTTGGGTTTACTCCGAATGCGAAAACATATTATTTGACACTGGAGGCGCTTTGTAAGCGGTCGTTTATGGATTGGGCATGTTCTGTATGTGAGAAAATGCTTAAGAGTGGTGTGTTATCGGAGGGAGAACAGATGGGTAACATCATAACTTGGTTTTGTAAGGAAGGGAAGGCCGAAGAGGCTTATTCGGTTTATGAATTGGCAAAGACGAAAGAGAAATCTTTGCCTCCTAGATTTGTGGCTACTCTGATAACTGCACTCTGCAAGAATGATGGGACTATCACGTTTGCTCAGGAGATGTTGGGTGATTTATCTGGAGAAGCTAGACGACGTGGGATAAAACCATTTTCTGATGTTATCCATAGTTTATGCAGAATGAGAAATGTTAAAGACGCTAAAGCTTTGCTTTTAGATATGATTTCTAAAGGGCCTGCTCCTGGGAATGCGGTTTTTAATTTGGTTGTCCATGCTTGTTCGAAAACTGGAGATCTGGATGAAGCAAAAGAAGTTTTAAAGTTGATGGAGAGTAGAGGTTTGAAACCAGATGTGTACACATACACTGTCATCATTAGCGGTTATGCTAAAGGAGGGATGATGGATGAAGCTCAAGAGATTCTCGCAGAGGCGAAAAAGAAACATAAGAAGCTTAGTCCTGTGACATATCACGCACTCATCCGAGGCTATTGCAAGATTGAAGAATATGACGAAGCTCTGAAACTCTTGAACGAAATGGATCGTTTCGGGGTGCAGCCTAATGCTGATGAGTACAATAAGCTGATACAATCATTTTGCCTCAAAGCTCTGGATTGGGAAAAAGCAGAGGTGCTGTTTGAGGAGATGAAGCAGAAAGGCTTGCACTTGAATGCGATCAGTCAGGGGCTTATAAGGGCAGTTAAAGAGATGGAATCTGAAGCCAAGGTAACGGAAGATGGCAACTTACTTGCTGAAGCATAG

Protein Analysis

576

Amino Acids

63.84

Weight (kDa)

5.08

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 407 - 454 5.9e-13 PPR repeat family
PPR_3 PF13812 408 - 452 5.9e-07 Pentatricopeptide repeat domain
PPR PF01535 409 - 438 7.8e-06 PPR repeat
PPR_3 PF13812 429 - 485 3.5e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 436 - 467 3.3e-09 PPR repeat
PPR_2 PF13041 445 - 489 7.6e-08 PPR repeat family
TPR_24 PF23276 455 - 544 4.4e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 464 - 522 3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 477 - 503 3.6e-07 PPR repeat
PPR_2 PF13041 478 - 523 1.3e-11 PPR repeat family
PPR PF01535 478 - 508 3.4e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1661
Acc36I ACCTGC 1 cut(s) 214
AciI CCGC 5 cut(s) 234, 267, 835, 1223, 1350
AclWI GGATC 3 cut(s) 3, 211, 1518
AcsI RAATTY 3 cut(s) 504, 517, 688
AcuI CTGAAG 1 cut(s) 1707
AfaI GTAC 4 cut(s) 304, 399, 1328, 1544
AfiI CCNNNNNNNGG 3 cut(s) 181, 723, 1214
AflII CTTAAG 1 cut(s) 878
AflIII ACRYGT 1 cut(s) 614
AgsI TTSAA 9 cut(s) 251, 494, 535, 688, 703, 1315, 1472, 1501, 1639
AjiI CACGTC 1 cut(s) 1109
AjnI CCWGG 2 cut(s) 499, 1213
Alw26I GTCTC 2 cut(s) 42, 202
AlwI GGATC 3 cut(s) 3, 211, 1518
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 2 cut(s) 948, 1205
ApeKI GCWGC 2 cut(s) 746, 1525
ApoI RAATTY 3 cut(s) 504, 517, 688
ArsI GACNNNNNNTTYG 2 cut(s) 73, 105
Asp700I GAANNNNTTC 2 cut(s) 139, 588
AspLEI GCGC 2 cut(s) 451, 826
AspS9I GGNCC 1 cut(s) 1205
AsuHPI GGTGA 3 cut(s) 335, 674, 1094
AsuII TTCGAA 2 cut(s) 148, 1253
AvaI CYCGRG 1 cut(s) 122
BaeI ACNNNNGTAYC 2 cut(s) 294, 327
BanII GRGCYC 1 cut(s) 176
BarI GAAGNNNNNNTAC 2 cut(s) 573, 605
BauI CACGAG 1 cut(s) 169
BbsI GAAGAC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 733, 1537
BccI CCATC 7 cut(s) 323, 904, 1043, 1291, 1366, 1672, 1700
BcgI CGANNNNNNTGC 2 cut(s) 1443, 1477
BciT130I CCWGG 2 cut(s) 501, 1215
BcoDI GTCTC 2 cut(s) 42, 202
BfaI CTAG 3 cut(s) 606, 1008, 1101
BfmI CTRYAG 1 cut(s) 19
BfoI RGCGCY 1 cut(s) 827
BfrI CTTAAG 1 cut(s) 878
BfuAI ACCTGC 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 956
BglII AGATCT 1 cut(s) 1264
BisI GCNGC 3 cut(s) 234, 747, 1526
BlsI GCNGC 3 cut(s) 235, 748, 1527
Bme1390I CCNGG 2 cut(s) 501, 1215
BmeT110I CYCGRG 1 cut(s) 122
BmgBI CACGTC 1 cut(s) 1109
BmgT120I GGNCC 1 cut(s) 1205
BmrFI CCNGG 2 cut(s) 501, 1215
BmrI ACTGGG 1 cut(s) 188
BmsI GCATC 1 cut(s) 616
BmuI ACTGGG 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 80
BpmI CTGGAG 3 cut(s) 839, 1113, 1281
Bpu10I CCTNAGC 1 cut(s) 1068
Bpu14I TTCGAA 2 cut(s) 148, 1253
BpuEI CTTGAG 1 cut(s) 1368
BsaAI YACGTR 1 cut(s) 617
BsaBI GATNNNNATC 1 cut(s) 221
BsaJI CCNNGG 6 cut(s) 309, 429, 499, 1214, 1453, 1692
BsaXI ACNNNNNCTCC 2 cut(s) 160, 190
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 723, 1214
Bse1I ACTGG 3 cut(s) 194, 822, 1264
Bse8I GATNNNNATC 1 cut(s) 221
BseBI CCWGG 2 cut(s) 501, 1215
BseDI CCNNGG 6 cut(s) 309, 429, 499, 1214, 1453, 1692
BseGI GGATG 5 cut(s) 631, 1276, 1374, 1381, 1449
BseJI GATNNNNATC 1 cut(s) 221
BseLI CCNNNNNNNGG 3 cut(s) 181, 723, 1214
BseMII CTCAG 2 cut(s) 560, 1082
BseNI ACTGG 3 cut(s) 194, 822, 1264
BseRI GAGGAG 1 cut(s) 1625
BseXI GCAGC 2 cut(s) 733, 1537
BsgI GTGCAG 2 cut(s) 1017, 1544
Bsh1285I CGRYCG 1 cut(s) 839
BshFI GGCC 2 cut(s) 950, 1207
BsiEI CGRYCG 1 cut(s) 839
BsiHKCI CYCGRG 1 cut(s) 122
BslFI GGGAC 1 cut(s) 1066
BslI CCNNNNNNNGG 3 cut(s) 181, 723, 1214
BsmAI GTCTC 2 cut(s) 42, 202
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 1 cut(s) 1066
BsmI GAATGC 3 cut(s) 799, 1225, 1645
BsnI GGCC 2 cut(s) 950, 1207
BsoBI CYCGRG 1 cut(s) 122
Bsp119I TTCGAA 2 cut(s) 148, 1253
Bsp1286I GDGCHC 1 cut(s) 176
Bsp1407I TGTACA 1 cut(s) 1326
Bsp143I GATC 6 cut(s) 8, 23, 216, 1264, 1510, 1644
BspACI CCGC 5 cut(s) 234, 267, 835, 1223, 1350
BspANI GGCC 2 cut(s) 950, 1207
BspCNI CTCAG 2 cut(s) 559, 1081
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 3 cut(s) 3, 211, 1518
BspT104I TTCGAA 2 cut(s) 148, 1253
BspTI CTTAAG 1 cut(s) 878
BsrGI TGTACA 1 cut(s) 1326
BsrI ACTGG 3 cut(s) 194, 822, 1264
BssECI CCNNGG 6 cut(s) 309, 429, 499, 1214, 1453, 1692
BssMI GATC 6 cut(s) 8, 23, 216, 1264, 1510, 1644
BssSI CACGAG 1 cut(s) 169
BssT1I CCWWGG 2 cut(s) 429, 1692
Bst2BI CACGAG 1 cut(s) 169
Bst2UI CCWGG 2 cut(s) 501, 1215
Bst4CI ACNGT 3 cut(s) 402, 565, 1339
Bst6I CTCTTC 5 cut(s) 180, 549, 594, 768, 948
BstAFI CTTAAG 1 cut(s) 878
BstAUI TGTACA 1 cut(s) 1326
BstBAI YACGTR 1 cut(s) 617
BstBI TTCGAA 2 cut(s) 148, 1253
BstC8I GCNNGC 2 cut(s) 1209, 1631
BstDEI CTNAG 4 cut(s) 469, 546, 1068, 1424
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 5 cut(s) 631, 1276, 1374, 1381, 1449
BstH2I RGCGCY 1 cut(s) 827
BstHHI GCGC 2 cut(s) 451, 826
BstKTI GATC 6 cut(s) 11, 26, 219, 1267, 1513, 1647
BstMAI GTCTC 2 cut(s) 42, 202
BstMBI GATC 6 cut(s) 8, 23, 216, 1264, 1510, 1644
BstMCI CGRYCG 1 cut(s) 839
BstMWI GCNNNNNNNGC 2 cut(s) 832, 956
BstNI CCWGG 2 cut(s) 501, 1215
BstNSI RCATGY 1 cut(s) 858
BstSCI CCNGG 2 cut(s) 499, 1213
BstSFI CTRYAG 1 cut(s) 19
BstV1I GCAGC 2 cut(s) 733, 1537
BstV2I GAAGAC 1 cut(s) 80
BstX2I RGATCY 3 cut(s) 8, 216, 1264
BstYI RGATCY 3 cut(s) 8, 216, 1264
BsuRI GGCC 2 cut(s) 950, 1207
BtgI CCRYGG 1 cut(s) 309
BtrI CACGTC 1 cut(s) 1109
BtsCI GGATG 5 cut(s) 631, 1276, 1374, 1381, 1449
BtsIMutI CAGTG 2 cut(s) 815, 1335
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 2 cut(s) 1209, 1631
CfoI GCGC 2 cut(s) 451, 826
Cfr13I GGNCC 1 cut(s) 1205
CseI GACGC 2 cut(s) 24, 1178
Csp6I GTAC 4 cut(s) 303, 398, 1327, 1543
CspCI CAANNNNNGTGG 2 cut(s) 559, 594
CviAII CATG 2 cut(s) 855, 1244
CviQI GTAC 4 cut(s) 303, 398, 1327, 1543
DdeI CTNAG 4 cut(s) 469, 546, 1068, 1424
DpnI GATC 6 cut(s) 10, 25, 218, 1266, 1512, 1646
DpnII GATC 6 cut(s) 8, 23, 216, 1264, 1510, 1644
DraI TTTAAA 1 cut(s) 1290
Eam1104I CTCTTC 5 cut(s) 180, 549, 594, 768, 948
EarI CTCTTC 5 cut(s) 180, 549, 594, 768, 948
Eco130I CCWWGG 2 cut(s) 429, 1692
Eco24I GRGCYC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 1707
Eco88I CYCGRG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 1205
EcoRII CCWGG 2 cut(s) 499, 1213
EcoT14I CCWWGG 2 cut(s) 429, 1692
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 2 cut(s) 429, 1692
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 2 cut(s) 858, 1247
FalI AAGNNNNNCTT 2 cut(s) 1167, 1199
FaqI GGGAC 1 cut(s) 1066
FatI CATG 2 cut(s) 854, 1243
Fnu4HI GCNGC 3 cut(s) 234, 747, 1526
FokI GGATG 5 cut(s) 638, 1283, 1381, 1388, 1436
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 3 cut(s) 234, 747, 1526
FspBI CTAG 3 cut(s) 606, 1008, 1101
GlaI GCGC 2 cut(s) 450, 825
GluI GCNGC 3 cut(s) 234, 747, 1526
GsuI CTGGAG 3 cut(s) 839, 1113, 1281
HaeII RGCGCY 1 cut(s) 827
HaeIII GGCC 2 cut(s) 950, 1207
HgaI GACGC 2 cut(s) 24, 1178
HhaI GCGC 2 cut(s) 451, 826
Hin1II CATG 2 cut(s) 858, 1247
Hin6I GCGC 2 cut(s) 449, 824
HinP1I GCGC 2 cut(s) 449, 824
HindIII AAGCTT 2 cut(s) 1176, 1421
HphI GGTGA 3 cut(s) 335, 674, 1094
Hpy166II GTNNAC 4 cut(s) 367, 786, 1327, 1329
Hpy8I GTNNAC 4 cut(s) 367, 786, 1327, 1329
Hpy99I CGWCG 1 cut(s) 1110
HpyAV CCTTC 2 cut(s) 935, 940
HpyCH4III ACNGT 3 cut(s) 402, 565, 1339
HpyCH4IV ACGT 3 cut(s) 616, 1061, 1108
HpyCH4V TGCA 7 cut(s) 350, 1034, 1041, 1149, 1464, 1525, 1633
HpyF10VI GCNNNNNNNGC 2 cut(s) 832, 956
HpyF3I CTNAG 4 cut(s) 469, 546, 1068, 1424
HpySE526I ACGT 3 cut(s) 616, 1061, 1108
Hsp92II CATG 2 cut(s) 858, 1247
HspAI GCGC 2 cut(s) 449, 824
Kzo9I GATC 6 cut(s) 8, 23, 216, 1264, 1510, 1644
LmnI GCTCC 1 cut(s) 1216
Lsp1109I GCAGC 2 cut(s) 733, 1537
LweI GCATC 1 cut(s) 616
MaeI CTAG 3 cut(s) 606, 1008, 1101
MaeII ACGT 3 cut(s) 616, 1061, 1108
MaeIII GTNAC 7 cut(s) 52, 201, 408, 565, 914, 1432, 1696
MalI GATC 6 cut(s) 10, 25, 218, 1266, 1512, 1646
MboI GATC 6 cut(s) 8, 23, 216, 1264, 1510, 1644
MfeI CAATTG 1 cut(s) 600
MflI RGATCY 3 cut(s) 8, 216, 1264
MhlI GDGCHC 1 cut(s) 176
MluCI AATT 7 cut(s) 371, 504, 517, 600, 688, 974, 1231
MlyI GAGTC 2 cut(s) 79, 134
MroXI GAANNNNTTC 2 cut(s) 139, 588
MseI TTAA 8 cut(s) 444, 453, 651, 879, 1164, 1230, 1289, 1671
MslI CAYNNNNRTG 1 cut(s) 1638
MspCI CTTAAG 1 cut(s) 878
MspR9I CCNGG 2 cut(s) 501, 1215
MunI CAATTG 1 cut(s) 600
Mva1269I GAATGC 3 cut(s) 799, 1225, 1645
MvaI CCWGG 2 cut(s) 501, 1215
MwoI GCNNNNNNNGC 2 cut(s) 832, 956
NdeII GATC 6 cut(s) 8, 23, 216, 1264, 1510, 1644
NlaIII CATG 2 cut(s) 858, 1247
NmuCI GTSAC 1 cut(s) 1432
NspI RCATGY 1 cut(s) 858
NspV TTCGAA 2 cut(s) 148, 1253
PaeR7I CTCGAG 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PctI GAATGC 3 cut(s) 799, 1225, 1645
PdmI GAANNNNTTC 2 cut(s) 139, 588
PkrI GCNGC 3 cut(s) 235, 748, 1527
PleI GAGTC 2 cut(s) 79, 133
PpsI GAGTC 2 cut(s) 79, 133
Ppu21I YACGTR 1 cut(s) 617
PsiI TTATAA 1 cut(s) 1661
Psp6I CCWGG 2 cut(s) 499, 1213
PspGI CCWGG 2 cut(s) 499, 1213
PspPI GGNCC 1 cut(s) 1205
PsuI RGATCY 3 cut(s) 8, 216, 1264
RsaI GTAC 4 cut(s) 304, 399, 1328, 1544
RsaNI GTAC 4 cut(s) 303, 398, 1327, 1543
RseI CAYNNNNRTG 1 cut(s) 1638
SaqAI TTAA 8 cut(s) 444, 453, 651, 879, 1164, 1230, 1289, 1671
SatI GCNGC 3 cut(s) 234, 747, 1526
Sau3AI GATC 6 cut(s) 8, 23, 216, 1264, 1510, 1644
Sau96I GGNCC 1 cut(s) 1205
SchI GAGTC 2 cut(s) 79, 134
ScrFI CCNGG 2 cut(s) 501, 1215
SduI GDGCHC 1 cut(s) 176
SfaNI GCATC 1 cut(s) 616
SfcI CTRYAG 1 cut(s) 19
Sfr274I CTCGAG 1 cut(s) 122
SfuI TTCGAA 2 cut(s) 148, 1253
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 1638
SmlI CTYRAG 3 cut(s) 122, 878, 1383
SmoI CTYRAG 3 cut(s) 122, 878, 1383
Sse9I AATT 7 cut(s) 371, 504, 517, 600, 688, 974, 1231
SsiI CCGC 5 cut(s) 234, 267, 835, 1223, 1350
SspMI CTAG 3 cut(s) 606, 1008, 1101
StyD4I CCNGG 2 cut(s) 499, 1213
StyI CCWWGG 2 cut(s) 429, 1692
TaaI ACNGT 3 cut(s) 402, 565, 1339
TaiI ACGT 3 cut(s) 619, 1064, 1111
TaqI TCGA 7 cut(s) 58, 65, 107, 123, 148, 459, 1253
TaqII GACCGA 1 cut(s) 785
TasI AATT 7 cut(s) 371, 504, 517, 600, 688, 974, 1231
TatI WGTACW 3 cut(s) 397, 1326, 1542
TauI GCSGC 1 cut(s) 236
Tru1I TTAA 8 cut(s) 444, 453, 651, 879, 1164, 1230, 1289, 1671
Tru9I TTAA 8 cut(s) 444, 453, 651, 879, 1164, 1230, 1289, 1671
TscAI CASTG 2 cut(s) 822, 1342
TseFI GTSAC 1 cut(s) 1432
TseI GCWGC 2 cut(s) 746, 1525
Tsp45I GTSAC 1 cut(s) 1432
TspDTI ATGAA 8 cut(s) 105, 435, 567, 720, 987, 1287, 1392, 1631
TspGWI ACGGA 4 cut(s) 197, 298, 427, 1715
TspRI CASTG 2 cut(s) 822, 1342
Vha464I CTTAAG 1 cut(s) 878
XapI RAATTY 3 cut(s) 504, 517, 688
XceI RCATGY 1 cut(s) 858
XhoI CTCGAG 1 cut(s) 122
XmnI GAANNNNTTC 2 cut(s) 139, 588
XspI CTAG 3 cut(s) 606, 1008, 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.