Rw2G028540

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011325
Physical Location & Seq
Reverse (-)
45716382 .. 45718661
2280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028540.1

Sequence Viewer

Length: 1716 bp
ATGTGGAGGTCAGTCGCGGCAAGATCATCAAGAGCTTCCCTTTCGGCAAAGGCAGCACACAACTTCGGTGCAACCCAAAACCCCAAGGTACTCTCCTCTAAAACCCTAGTCCCACTTGCCCAATCTTCTCATTTCCCCGCTAGATCCCCTCCCTGCCAAAACCCCAGGTTCTTCTCTCAGGTCTCAGAAGACCTTAGTTATTCTGATACCGCAGAACCACCGCCGCCACCGTTTTCAGAAAATGACGACGCCCAGATGGACGGTTTTGCCCCTGGGGATGGGGTTGATGGGGGTTCGGAGCAACTGGGTTTTGGTGAGATTGGGGAGGTAGGGGAAGAGGAAGAAGAGGTTCATGAGATTGATGTGGAGAAATTGGAGGGTTTGTTGTCTCTGCTTCATAGTAGTGCTGATGGGTCTCTTGAGTCTAGTTTCAGGGATTTGGATTTGACTCTACATGAAGAATTTGTGGTTAGAGTTCTTGAAACCCCACTTATTGTGGGGGAGAATTTGTTAAGGTTCTTCAAGTGGGGTTTGAAAGAGAAACCCGAATTTAGAGTCAATAAGCAGATATTGGATGCTCTTGTGATTGCAATGTGTGGTGAAGTTGTTACCAAGAAAGATATGTATTCTTTGTGGGATTTGGTTAAGGGGGTTGGTGTGAAGGAGGGGAGTTTGTTGAATGTGGATATTCTCAATGTGTTGATGTCTTCGTTCGCGAAATTGGGTGAAGGGAAAACTGCTTTGGAGGTGTTGAACAAGTTTGATGATTTTGGATGCGTCCCCAATGCGGATTCATATTACATTGCGATTGAGGGGCTTTGCAGGAGTTCGGATTTTGATTGTGCTAAGTCTGTTTCTGAGAAGATGATTTGTGCAAGAAGCTCCCCGGATGCTGAAAAGGTTGGTAGGATCGTGTCTTGGTTCTGCAAGGCTGGAATGGCGAAAGAGGCCCATTCGGTGTATTTGTTGTTGAAGGAGGAGAAGCAGTGCCGACCTCGCTCATCTGTTCATTTTTTGATCCGGTCACTTAGTAGGGAGGATGAAAATGTCAAATTAGCTCTAGACTTGCTAGAGGATATTGATGGTGAAGCACGGAAATATGCGATAAAGCCCTTTTCTGCTGTTGTTCAAGGGTTATGTAGGATAAAGGATGTTGCTGGGGCAAGAATATTGCTTCTTGAGATGGCAAAAAAGGGTCCACCTCCTGGAAATGCAGTTTTTAATTCGGTTATCAATGGCTATTCTAAGGCTGGGGATATGCAAGATGCGATAGAGATGATGAAGCTGATGGAGAGTAGGGGCTTGAAACCAGATGTCTTTACGTACACTGTTATTATGAGTGGTTATGCAAAAGGTGGTCAGATGGAGGAGGCATGCAATATCTTATCTGAAGCCAAAAAGAAGCATGCCAAATTGACTCCAGTGACTTATCATACACTCATCTCCAGCTTTTGCAAACTGGAACAGTTTGACAAGGCTTTGGAGTTGTTTCATGAGATGAAAGATTATGGGGTCCAACCCACGACTGATGAGTACAACAAGTTGATCCAATCTCTTTGCCTGAAAGCACTAGATTGGGAAACGTCAGAGAAACTGCTAGAAGAAATGAAAGATAGCGGGTTGTATCTTAATGGAATTACGAGAGGTCTCATAAGAGCAGTCAAGGAACTGCAGACGGAGAAAGTAGAGACTGAGAAAATAGTTGCAGAAGCATAA

Protein Analysis

571

Amino Acids

63.16

Weight (kDa)

5.16

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 227 - 275 2.1e-06 PPR repeat family
PPR_long PF17177 378 - 521 1.9e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 394 - 449 3.5e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 404 - 451 3.8e-15 PPR repeat family
PPR PF01535 406 - 435 1.7e-07 PPR repeat
PPR_1 PF12854 433 - 464 2.8e-07 PPR repeat
PPR_2 PF13041 443 - 486 8.1e-08 PPR repeat family
TPR_24 PF23276 450 - 540 5.7e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 461 - 517 5.8e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 471 - 501 3.7e-08 PPR repeat
PPR_2 PF13041 475 - 520 1.1e-11 PPR repeat family
PPR PF01535 475 - 505 4.7e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1205
AccII CGCG 2 cut(s) 17, 716
AciI CCGC 7 cut(s) 17, 138, 210, 221, 224, 788, 1617
AclWI GGATC 4 cut(s) 138, 917, 1012, 1540
AcsI RAATTY 3 cut(s) 461, 505, 548
AcuI CTGAAG 1 cut(s) 1410
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 3 cut(s) 90, 1325, 1535
AfiI CCNNNNNNNGG 3 cut(s) 278, 787, 1205
AgsI TTSAA 8 cut(s) 482, 523, 535, 679, 754, 973, 1130, 1306
AjnI CCWGG 3 cut(s) 164, 271, 1204
AjuI GAANNNNNNNTTGG 2 cut(s) 725, 757
AluBI AGCT 5 cut(s) 35, 882, 1058, 1285, 1449
AluI AGCT 5 cut(s) 35, 882, 1058, 1285, 1449
Alw26I GTCTC 5 cut(s) 187, 393, 420, 1652, 1682
AlwI GGATC 4 cut(s) 138, 917, 1012, 1540
AoxI GGCC 1 cut(s) 948
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 3 cut(s) 461, 505, 548
ArsI GACNNNNNNTTYG 2 cut(s) 540, 572
Asp700I GAANNNNTTC 1 cut(s) 348
AspS9I GGNCC 3 cut(s) 949, 1196, 1513
AsuC2I CCSGG 1 cut(s) 887
AsuHPI GGTGA 4 cut(s) 326, 611, 737, 1097
AvaII GGWCC 2 cut(s) 1196, 1513
BbsI GAAGAC 2 cut(s) 195, 699
BbvI GCAGC 1 cut(s) 65
BccI CCATC 8 cut(s) 250, 272, 281, 404, 1076, 1177, 1282, 1357
BciT130I CCWGG 3 cut(s) 166, 273, 1206
BcnI CCSGG 1 cut(s) 887
BcoDI GTCTC 5 cut(s) 187, 393, 420, 1652, 1682
BfaI CTAG 7 cut(s) 107, 141, 426, 1061, 1070, 1571, 1598
BfmI CTRYAG 1 cut(s) 1670
BisI GCNGC 3 cut(s) 18, 54, 224
BlsI GCNGC 3 cut(s) 19, 55, 225
Bme1390I CCNGG 4 cut(s) 166, 273, 887, 1206
Bme18I GGWCC 2 cut(s) 1196, 1513
BmgT120I GGNCC 3 cut(s) 949, 1196, 1513
BmiI GGNNCC 2 cut(s) 1197, 1514
BmrFI CCNGG 4 cut(s) 166, 273, 887, 1206
BmrI ACTGGG 1 cut(s) 314
BmsI GCATC 4 cut(s) 565, 764, 880, 1255
BmuI ACTGGG 1 cut(s) 314
BpiI GAAGAC 2 cut(s) 195, 699
BpmI CTGGAG 2 cut(s) 1404, 1429
BpuEI CTTGAG 2 cut(s) 440, 1199
BpuMI CCSGG 1 cut(s) 887
BsaAI YACGTR 1 cut(s) 1323
BsaHI GRCGYC 1 cut(s) 249
BsaI GGTCTC 3 cut(s) 187, 420, 1652
BsaJI CCNNGG 5 cut(s) 84, 164, 271, 272, 885
BsaWI WCCGGW 1 cut(s) 1020
BsaXI ACNNNNNCTCC 6 cut(s) 368, 398, 494, 524, 971, 1001
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 787, 1205
Bse1I ACTGG 3 cut(s) 309, 1421, 1464
Bse3DI GCAATG 2 cut(s) 597, 801
BseBI CCWGG 3 cut(s) 166, 273, 1206
BseDI CCNNGG 5 cut(s) 84, 164, 271, 272, 885
BseGI GGATG 6 cut(s) 283, 580, 779, 895, 1045, 1156
BseLI CCNNNNNNNGG 3 cut(s) 278, 787, 1205
BseMI GCAATG 2 cut(s) 597, 801
BseMII CTCAG 4 cut(s) 191, 198, 849, 1683
BseNI ACTGG 3 cut(s) 309, 1421, 1464
BseRI GAGGAG 3 cut(s) 85, 992, 1382
BseXI GCAGC 1 cut(s) 65
BseYI CCCAGC 2 cut(s) 1157, 1250
Bsh1236I CGCG 2 cut(s) 17, 716
BshFI GGCC 1 cut(s) 950
BsiSI CCGG 2 cut(s) 887, 1021
BslFI GGGAC 2 cut(s) 95, 764
BslI CCNNNNNNNGG 3 cut(s) 278, 787, 1205
BsmAI GTCTC 5 cut(s) 187, 393, 420, 1652, 1682
BsmFI GGGAC 2 cut(s) 95, 764
BsnI GGCC 1 cut(s) 950
Bso31I GGTCTC 3 cut(s) 187, 420, 1652
Bsp143I GATC 5 cut(s) 23, 143, 909, 1017, 1545
Bsp68I TCGCGA 1 cut(s) 716
BspACI CCGC 7 cut(s) 17, 138, 210, 221, 224, 788, 1617
BspANI GGCC 1 cut(s) 950
BspCNI CTCAG 4 cut(s) 190, 197, 850, 1684
BspFNI CGCG 2 cut(s) 17, 716
BspHI TCATGA 2 cut(s) 352, 1492
BspLI GGNNCC 2 cut(s) 1197, 1514
BspMAI CTGCAG 1 cut(s) 1674
BspPI GGATC 4 cut(s) 138, 917, 1012, 1540
BspTNI GGTCTC 3 cut(s) 187, 420, 1652
BsrDI GCAATG 2 cut(s) 597, 801
BsrI ACTGG 3 cut(s) 309, 1421, 1464
BssECI CCNNGG 5 cut(s) 84, 164, 271, 272, 885
BssMI GATC 5 cut(s) 23, 143, 909, 1017, 1545
BssNI GRCGYC 1 cut(s) 249
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 3 cut(s) 166, 273, 1206
Bst4CI ACNGT 4 cut(s) 231, 263, 1330, 1467
Bst6I CTCTTC 2 cut(s) 330, 339
BstACI GRCGYC 1 cut(s) 249
BstBAI YACGTR 1 cut(s) 1323
BstC8I GCNNGC 2 cut(s) 1375, 1407
BstDEI CTNAG 8 cut(s) 177, 184, 194, 846, 858, 1028, 1245, 1692
BstF5I GGATG 6 cut(s) 283, 580, 779, 895, 1045, 1156
BstFNI CGCG 2 cut(s) 17, 716
BstKTI GATC 5 cut(s) 26, 146, 912, 1020, 1548
BstMAI GTCTC 5 cut(s) 187, 393, 420, 1652, 1682
BstMBI GATC 5 cut(s) 23, 143, 909, 1017, 1545
BstMWI GCNNNNNNNGC 4 cut(s) 53, 938, 947, 996
BstNI CCWGG 3 cut(s) 166, 273, 1206
BstNSI RCATGY 2 cut(s) 1377, 1409
BstSCI CCNGG 4 cut(s) 164, 271, 885, 1204
BstSFI CTRYAG 1 cut(s) 1670
BstSNI TACGTA 1 cut(s) 1323
BstUI CGCG 2 cut(s) 17, 716
BstV1I GCAGC 1 cut(s) 65
BstV2I GAAGAC 2 cut(s) 195, 699
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BsuRI GGCC 1 cut(s) 950
BtsCI GGATG 6 cut(s) 283, 580, 779, 895, 1045, 1156
BtsI GCAGTG 1 cut(s) 992
BtsIMutI CAGTG 3 cut(s) 992, 1326, 1428
BtuMI TCGCGA 1 cut(s) 716
Cac8I GCNNGC 2 cut(s) 1375, 1407
CciI TCATGA 2 cut(s) 352, 1492
Cfr13I GGNCC 3 cut(s) 949, 1196, 1513
CseI GACGC 2 cut(s) 257, 766
Csp6I GTAC 3 cut(s) 89, 1324, 1534
CviAII CATG 5 cut(s) 353, 455, 1374, 1406, 1493
CviQI GTAC 3 cut(s) 89, 1324, 1534
DdeI CTNAG 8 cut(s) 177, 184, 194, 846, 858, 1028, 1245, 1692
DpnI GATC 5 cut(s) 25, 145, 911, 1019, 1547
DpnII GATC 5 cut(s) 23, 143, 909, 1017, 1545
Eam1104I CTCTTC 2 cut(s) 330, 339
EarI CTCTTC 2 cut(s) 330, 339
Eco105I TACGTA 1 cut(s) 1323
Eco130I CCWWGG 1 cut(s) 84
Eco31I GGTCTC 3 cut(s) 187, 420, 1652
Eco47I GGWCC 2 cut(s) 1196, 1513
Eco57I CTGAAG 1 cut(s) 1410
EcoRII CCWGG 3 cut(s) 164, 271, 1204
EcoT14I CCWWGG 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 5 cut(s) 356, 458, 1377, 1409, 1496
FaqI GGGAC 2 cut(s) 95, 764
FatI CATG 5 cut(s) 352, 454, 1373, 1405, 1492
FauI CCCGC 2 cut(s) 145, 1610
Fnu4HI GCNGC 3 cut(s) 18, 54, 224
FokI GGATG 6 cut(s) 290, 587, 786, 902, 1052, 1163
Fsp4HI GCNGC 3 cut(s) 18, 54, 224
FspBI CTAG 7 cut(s) 107, 141, 426, 1061, 1070, 1571, 1598
GluI GCNGC 3 cut(s) 18, 54, 224
GsaI CCCAGC 2 cut(s) 1161, 1254
GsuI CTGGAG 2 cut(s) 1404, 1429
HaeIII GGCC 1 cut(s) 950
HapII CCGG 2 cut(s) 887, 1021
HgaI GACGC 2 cut(s) 257, 766
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 5 cut(s) 356, 458, 1377, 1409, 1496
HinfI GANTC 5 cut(s) 422, 448, 555, 791, 1417
HpaII CCGG 2 cut(s) 887, 1021
HphI GGTGA 4 cut(s) 326, 611, 737, 1097
Hpy166II GTNNAC 2 cut(s) 1199, 1326
Hpy188I TCNGA 9 cut(s) 187, 205, 238, 298, 832, 859, 1362, 1390, 1588
Hpy188III TCNNGA 8 cut(s) 30, 353, 419, 479, 715, 1061, 1178, 1493
Hpy8I GTNNAC 2 cut(s) 1199, 1326
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 3 cut(s) 655, 722, 967
HpyCH4III ACNGT 4 cut(s) 231, 263, 1330, 1467
HpyCH4IV ACGT 2 cut(s) 1322, 1583
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 938, 947, 996
HpyF3I CTNAG 8 cut(s) 177, 184, 194, 846, 858, 1028, 1245, 1692
HpySE526I ACGT 2 cut(s) 1322, 1583
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 5 cut(s) 356, 458, 1377, 1409, 1496
Kzo9I GATC 5 cut(s) 23, 143, 909, 1017, 1545
LmnI GCTCC 2 cut(s) 298, 887
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 4 cut(s) 565, 764, 880, 1255
MaeI CTAG 7 cut(s) 107, 141, 426, 1061, 1070, 1571, 1598
MaeII ACGT 2 cut(s) 1322, 1583
MaeIII GTNAC 3 cut(s) 607, 1023, 1423
MalI GATC 5 cut(s) 25, 145, 911, 1019, 1547
MboI GATC 5 cut(s) 23, 143, 909, 1017, 1545
MflI RGATCY 1 cut(s) 143
MluCI AATT 9 cut(s) 371, 461, 505, 548, 719, 1052, 1222, 1412, 1635
MlyI GAGTC 4 cut(s) 431, 442, 564, 1411
MmeI TCCRAC 1 cut(s) 1540
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 4 cut(s) 512, 645, 1221, 1629
MslI CAYNNNNRTG 1 cut(s) 402
MspI CCGG 2 cut(s) 887, 1021
MspR9I CCNGG 4 cut(s) 166, 273, 887, 1206
MvaI CCWGG 3 cut(s) 166, 273, 1206
MvnI CGCG 2 cut(s) 17, 716
MwoI GCNNNNNNNGC 4 cut(s) 53, 938, 947, 996
NciI CCSGG 1 cut(s) 887
NdeII GATC 5 cut(s) 23, 143, 909, 1017, 1545
NlaIII CATG 5 cut(s) 356, 458, 1377, 1409, 1496
NlaIV GGNNCC 2 cut(s) 1197, 1514
NmuCI GTSAC 2 cut(s) 1023, 1423
NruI TCGCGA 1 cut(s) 716
NspI RCATGY 2 cut(s) 1377, 1409
PaeI GCATGC 2 cut(s) 1377, 1409
PagI TCATGA 2 cut(s) 352, 1492
PasI CCCWGGG 1 cut(s) 272
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 791
PflMI CCANNNNNTGG 1 cut(s) 1205
PfoI TCCNGGA 1 cut(s) 1204
PkrI GCNGC 3 cut(s) 19, 55, 225
PleI GAGTC 4 cut(s) 430, 442, 563, 1411
PpsI GAGTC 4 cut(s) 430, 442, 563, 1411
Ppu21I YACGTR 1 cut(s) 1323
Psp6I CCWGG 3 cut(s) 164, 271, 1204
PspFI CCCAGC 2 cut(s) 1157, 1250
PspGI CCWGG 3 cut(s) 164, 271, 1204
PspN4I GGNNCC 2 cut(s) 1197, 1514
PspPI GGNCC 3 cut(s) 949, 1196, 1513
PstI CTGCAG 1 cut(s) 1674
PsuI RGATCY 1 cut(s) 143
RruI TCGCGA 1 cut(s) 716
RsaI GTAC 3 cut(s) 90, 1325, 1535
RsaNI GTAC 3 cut(s) 89, 1324, 1534
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 4 cut(s) 512, 645, 1221, 1629
SatI GCNGC 3 cut(s) 18, 54, 224
Sau3AI GATC 5 cut(s) 23, 143, 909, 1017, 1545
Sau96I GGNCC 3 cut(s) 949, 1196, 1513
SchI GAGTC 4 cut(s) 431, 442, 564, 1411
ScrFI CCNGG 4 cut(s) 166, 273, 887, 1206
SfaNI GCATC 4 cut(s) 565, 764, 880, 1255
SfcI CTRYAG 1 cut(s) 1670
SinI GGWCC 2 cut(s) 1196, 1513
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 2 cut(s) 419, 1178
SmoI CTYRAG 2 cut(s) 419, 1178
SnaBI TACGTA 1 cut(s) 1323
SphI GCATGC 2 cut(s) 1377, 1409
Sse9I AATT 9 cut(s) 371, 461, 505, 548, 719, 1052, 1222, 1412, 1635
SsiI CCGC 7 cut(s) 17, 138, 210, 221, 224, 788, 1617
SspI AATATT 1 cut(s) 1170
SspMI CTAG 7 cut(s) 107, 141, 426, 1061, 1070, 1571, 1598
StyD4I CCNGG 4 cut(s) 164, 271, 885, 1204
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 4 cut(s) 231, 263, 1330, 1467
TaiI ACGT 2 cut(s) 1325, 1586
TasI AATT 9 cut(s) 371, 461, 505, 548, 719, 1052, 1222, 1412, 1635
TatI WGTACW 1 cut(s) 1533
TauI GCSGC 2 cut(s) 20, 226
TfiI GAWTC 1 cut(s) 791
Tru1I TTAA 4 cut(s) 512, 645, 1221, 1629
Tru9I TTAA 4 cut(s) 512, 645, 1221, 1629
TscAI CASTG 3 cut(s) 992, 1333, 1428
TseFI GTSAC 2 cut(s) 1023, 1423
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 2 cut(s) 1023, 1423
TspGWI ACGGA 2 cut(s) 1108, 1691
TspRI CASTG 3 cut(s) 992, 1333, 1428
Van91I CCANNNNNTGG 1 cut(s) 1205
VpaK11BI GGWCC 2 cut(s) 1196, 1513
XapI RAATTY 3 cut(s) 461, 505, 548
XbaI TCTAGA 1 cut(s) 1060
XceI RCATGY 2 cut(s) 1377, 1409
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 7 cut(s) 107, 141, 426, 1061, 1070, 1571, 1598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.