AT3G04290

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010703
Physical Location & Seq
Reverse (-)
1133320 .. 1136397
3078 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G04290.1

Sequence Viewer

Length: 1101 bp
ATGAATATCAATTGTTCTCCATTAGGGTTTCTAATTTCACTCTTCTTCATTGTTACTTTTCTTGCACCACAAGTCAAATCTAGGGCTTTCTTTGTCTTTGGTGACTCTCTCGTCGACAATGGAAACAACGACTACCTCGTCACCACTGCCCGTGCCGACAATTACCCGTATGGTATCGACTACCCGACCCGTAGACCCACCGGACGTTTCTCTAACGGCCTTAACATTCCCGACATTATCAGTGAGGCTATAGGCATGCCGTCGACTTTGCCGTACCTTAGCCCGCATCTCACCGGAGAAAATCTTCTAGTCGGTGCTAATTTTGCCTCCGCAGGAATTGGAATCCTCAACGACACTGGAATTCAATTCGTAAACATAATTAGAATTTCTAAACAGATGGAATACTTCGAACAGTACCAGCTACGAGTGAGTGCATTGATCGGACCAGAAGCTACACAACAGCTAGTTAACCAAGCTCTTGTCCTAATTACACTCGGTGGTAATGATTTCGTCAACAATTATTATCTAATTCCGTTTTCAGCTCGTTCTCGCCAATACGCGTTACCAGACTACGTTGTGTATCTCATTTCCGAATACGGAAAGATTCTCAGAAAACTCTACGAACTGGGTGCAAGACGAGTTTTGGTAACTGGAACTGGTGCAATGGGATGTGCTCCCGCGGAGCTGGCTCAGCATAGCCGCAATGGCGAGTGCTATGGCGCCCTCCAAACAGCAGCCGCTTTGTTCAATCCGCAATTAGTTGATTTGATCGCATCAGTCAATGCTGAAATTGGTCAAGACGTGTTTGTAGCAGCCAATGCCTATCAAATGAACATGGATTATCTATCTAATCCTGAACAATTTGGGTTTGTGACATCGAAGGTGGCATGTTGTGGACAAGGACCGTACAATGGCATAGGACTATGCACACCAGTCTCGAATTTGTGTCCGAATAGAGACTTATATGCGTTTTGGGATGCGTTTCATCCGACAGAGAAAGCCAATAGGATCATCGTTAATCAGATCCTGACTGGTTCCTCCAAGTATATGCACCCTATGAATCTTAGCACGGCCATGCTATTGGATTCTTCTAAAATCTAG

Protein Analysis

366

Amino Acids

40.15

Weight (kDa)

5.6

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 342 5.8e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04290 AT5G33370 AT5G33370
fragaria_vesca FvH4_6g01390 FvH4_6g01390
malus_domestica MD04G1234300.v1.1 MD12G1252200.v1.1
prunus_persica Prupe.6G354100_v2.0.a1
pyrus_communis pycom04g20800
rosa_chinensis RchiOBHm_Chr3g0448981 RchiOBHm_Chr3g0449021
rosa_laevigata RLG00000025862
rosa_multiflora Rmu_sc0003116.1_g000011
rosa_roxburghii Rroxscaffold_6G00427140
rosa_rugosa Rorug02G0617400
rosa_samantha Rh3AG015900 Rh3BG016000 Rh3CG015000 Rh3DG016300
rosa_wichuraiana Rw3G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 110, 137
AccB1I GGYRCC 1 cut(s) 719
AccI GTMKAC 3 cut(s) 114, 193, 263
AccII CGCG 2 cut(s) 560, 680
AciI CCGC 7 cut(s) 284, 330, 678, 680, 700, 738, 752
AclWI GGATC 2 cut(s) 1016, 1018
AcoI YGGCCR 1 cut(s) 1071
AcsI RAATTY 3 cut(s) 360, 384, 940
AcyI GRCGYC 1 cut(s) 720
AdeI CACNNNGTG 1 cut(s) 497
AfaI GTAC 3 cut(s) 275, 416, 908
AfiI CCNNNNNNNGG 1 cut(s) 911
AflIII ACRYGT 2 cut(s) 558, 801
AgsI TTSAA 2 cut(s) 365, 748
AjiI CACGTC 1 cut(s) 802
AluBI AGCT 6 cut(s) 421, 452, 463, 476, 542, 685
AluI AGCT 6 cut(s) 421, 452, 463, 476, 542, 685
Alw21I GWGCWC 1 cut(s) 676
Alw26I GTCTC 2 cut(s) 940, 951
AlwI GGATC 2 cut(s) 1016, 1018
AlwNI CAGNNNCTG 1 cut(s) 1027
AoxI GGCC 2 cut(s) 217, 1071
ApeKI GCWGC 2 cut(s) 734, 812
ApoI RAATTY 3 cut(s) 360, 384, 940
Asp700I GAANNNNTTC 1 cut(s) 303
AspLEI GCGC 1 cut(s) 722
AspS9I GGNCC 2 cut(s) 443, 902
AsuHPI GGTGA 3 cut(s) 113, 133, 283
AsuII TTCGAA 1 cut(s) 408
AvaII GGWCC 2 cut(s) 443, 902
BanI GGYRCC 1 cut(s) 719
Bbv12I GWGCWC 1 cut(s) 676
BbvI GCAGC 2 cut(s) 746, 824
BccI CCATC 1 cut(s) 391
BceAI ACGGC 4 cut(s) 232, 244, 256, 1086
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BcoDI GTCTC 2 cut(s) 940, 951
BfaI CTAG 4 cut(s) 81, 308, 464, 1099
BfmI CTRYAG 1 cut(s) 249
BfoI RGCGCY 1 cut(s) 723
BglI GCCNNNNNGGC 1 cut(s) 705
BisI GCNGC 4 cut(s) 700, 735, 738, 813
BlpI GCTNAGC 1 cut(s) 690
BlsI GCNGC 4 cut(s) 701, 736, 739, 814
Bme18I GGWCC 2 cut(s) 443, 902
BmgBI CACGTC 1 cut(s) 802
BmgT120I GGNCC 2 cut(s) 443, 902
BmiI GGNNCC 2 cut(s) 721, 1036
BmrI ACTGGG 1 cut(s) 635
BmsI GCATC 3 cut(s) 295, 782, 967
BmuI ACTGGG 1 cut(s) 635
Bpu10I CCTNAGC 1 cut(s) 278
Bpu1102I GCTNAGC 1 cut(s) 690
Bpu14I TTCGAA 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 720
BsaJI CCNNGG 1 cut(s) 678
BsaWI WCCGGW 2 cut(s) 200, 293
Bsc4I CCNNNNNNNGG 1 cut(s) 911
Bse1I ACTGG 6 cut(s) 361, 630, 655, 661, 932, 1036
Bse3DI GCAATG 2 cut(s) 669, 709
BseDI CCNNGG 1 cut(s) 678
BseGI GGATG 3 cut(s) 674, 982, 985
BseLI CCNNNNNNNGG 1 cut(s) 911
BseMI GCAATG 2 cut(s) 669, 709
BseMII CTCAG 2 cut(s) 622, 704
BseNI ACTGG 6 cut(s) 361, 630, 655, 661, 932, 1036
BseXI GCAGC 2 cut(s) 746, 824
Bsh1236I CGCG 2 cut(s) 560, 680
BshFI GGCC 2 cut(s) 219, 1073
BshNI GGYRCC 1 cut(s) 719
BsiHKAI GWGCWC 1 cut(s) 676
BsiSI CCGG 2 cut(s) 201, 294
BslI CCNNNNNNNGG 1 cut(s) 911
BsmAI GTCTC 2 cut(s) 940, 951
BsnI GGCC 2 cut(s) 219, 1073
Bsp119I TTCGAA 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 676
Bsp143I GATC 4 cut(s) 438, 768, 1008, 1023
Bsp1720I GCTNAGC 1 cut(s) 690
BspACI CCGC 7 cut(s) 284, 330, 678, 680, 700, 738, 752
BspANI GGCC 2 cut(s) 219, 1073
BspCNI CTCAG 2 cut(s) 621, 703
BspFNI CGCG 2 cut(s) 560, 680
BspLI GGNNCC 2 cut(s) 721, 1036
BspPI GGATC 2 cut(s) 1016, 1018
BspT104I TTCGAA 1 cut(s) 408
BspT107I GGYRCC 1 cut(s) 719
BsrDI GCAATG 2 cut(s) 669, 709
BsrI ACTGG 6 cut(s) 361, 630, 655, 661, 932, 1036
BssECI CCNNGG 1 cut(s) 678
BssMI GATC 4 cut(s) 438, 768, 1008, 1023
BssNI GRCGYC 1 cut(s) 720
Bst4CI ACNGT 2 cut(s) 414, 906
Bst6I CTCTTC 1 cut(s) 47
BstACI GRCGYC 1 cut(s) 720
BstAPI GCANNNNNTGC 1 cut(s) 818
BstBI TTCGAA 1 cut(s) 408
BstC8I GCNNGC 3 cut(s) 257, 284, 687
BstDEI CTNAG 4 cut(s) 278, 608, 690, 1064
BstDSI CCRYGG 1 cut(s) 678
BstF5I GGATG 3 cut(s) 674, 982, 985
BstFNI CGCG 2 cut(s) 560, 680
BstH2I RGCGCY 1 cut(s) 723
BstHHI GCGC 1 cut(s) 722
BstKTI GATC 4 cut(s) 441, 771, 1011, 1026
BstMAI GTCTC 2 cut(s) 940, 951
BstMBI GATC 4 cut(s) 438, 768, 1008, 1023
BstMWI GCNNNNNNNGC 5 cut(s) 323, 686, 691, 705, 818
BstNSI RCATGY 2 cut(s) 259, 891
BstSFI CTRYAG 1 cut(s) 249
BstUI CGCG 2 cut(s) 560, 680
BstV1I GCAGC 2 cut(s) 746, 824
BstX2I RGATCY 1 cut(s) 1023
BstXI CCANNNNNNTGG 1 cut(s) 1081
BstYI RGATCY 1 cut(s) 1023
BsuRI GGCC 2 cut(s) 219, 1073
BtgI CCRYGG 1 cut(s) 678
BtrI CACGTC 1 cut(s) 802
BtsCI GGATG 3 cut(s) 674, 982, 985
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 3 cut(s) 144, 247, 354
Cac8I GCNNGC 3 cut(s) 257, 284, 687
CaiI CAGNNNCTG 1 cut(s) 1027
CfoI GCGC 1 cut(s) 722
Cfr13I GGNCC 2 cut(s) 443, 902
Cfr42I CCGCGG 1 cut(s) 681
Csp6I GTAC 3 cut(s) 274, 415, 907
CviAII CATG 4 cut(s) 256, 835, 888, 1075
CviQI GTAC 3 cut(s) 274, 415, 907
DdeI CTNAG 4 cut(s) 278, 608, 690, 1064
DinI GGCGCC 1 cut(s) 721
DpnI GATC 4 cut(s) 440, 770, 1010, 1025
DpnII GATC 4 cut(s) 438, 768, 1008, 1023
DraIII CACNNNGTG 1 cut(s) 497
DrdI GACNNNNNNGTC 2 cut(s) 110, 137
DseDI GACNNNNNNGTC 2 cut(s) 110, 137
EaeI YGGCCR 1 cut(s) 1071
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Eco47I GGWCC 2 cut(s) 443, 902
EcoRI GAATTC 1 cut(s) 360
EgeI GGCGCC 1 cut(s) 721
EheI GGCGCC 1 cut(s) 721
FaeI CATG 4 cut(s) 259, 838, 891, 1078
FatI CATG 4 cut(s) 255, 834, 887, 1074
FauI CCCGC 2 cut(s) 291, 685
FblI GTMKAC 3 cut(s) 114, 193, 263
Fnu4HI GCNGC 4 cut(s) 700, 735, 738, 813
FokI GGATG 3 cut(s) 681, 972, 989
Fsp4HI GCNGC 4 cut(s) 700, 735, 738, 813
FspBI CTAG 4 cut(s) 81, 308, 464, 1099
GlaI GCGC 1 cut(s) 721
GluI GCNGC 4 cut(s) 700, 735, 738, 813
HaeII RGCGCY 1 cut(s) 723
HaeIII GGCC 2 cut(s) 219, 1073
HapII CCGG 2 cut(s) 201, 294
HhaI GCGC 1 cut(s) 722
Hin1I GRCGYC 1 cut(s) 720
Hin1II CATG 4 cut(s) 259, 838, 891, 1078
Hin6I GCGC 1 cut(s) 720
HinP1I GCGC 1 cut(s) 720
HincII GTYRAC 4 cut(s) 115, 264, 469, 514
HindII GTYRAC 4 cut(s) 115, 264, 469, 514
HinfI GANTC 5 cut(s) 104, 342, 604, 1060, 1085
HpaI GTTAAC 1 cut(s) 469
HpaII CCGG 2 cut(s) 201, 294
HphI GGTGA 3 cut(s) 113, 133, 283
Hpy166II GTNNAC 7 cut(s) 115, 194, 264, 373, 469, 514, 896
Hpy188I TCNGA 6 cut(s) 443, 592, 611, 951, 990, 1023
Hpy188III TCNNGA 5 cut(s) 230, 797, 854, 937, 1027
Hpy8I GTNNAC 7 cut(s) 115, 194, 264, 373, 469, 514, 896
Hpy99I CGWCG 2 cut(s) 116, 265
HpyAV CCTTC 1 cut(s) 874
HpyCH4III ACNGT 2 cut(s) 414, 906
HpyCH4IV ACGT 3 cut(s) 205, 573, 801
HpyCH4V TGCA 6 cut(s) 65, 434, 632, 662, 927, 1051
HpyF10VI GCNNNNNNNGC 5 cut(s) 323, 686, 691, 705, 818
HpyF3I CTNAG 4 cut(s) 278, 608, 690, 1064
HpySE526I ACGT 3 cut(s) 205, 573, 801
Hsp92I GRCGYC 1 cut(s) 720
Hsp92II CATG 4 cut(s) 259, 838, 891, 1078
HspAI GCGC 1 cut(s) 720
KasI GGCGCC 1 cut(s) 719
KspAI GTTAAC 1 cut(s) 469
KspI CCGCGG 1 cut(s) 681
Kzo9I GATC 4 cut(s) 438, 768, 1008, 1023
LmnI GCTCC 2 cut(s) 679, 682
Lsp1109I GCAGC 2 cut(s) 746, 824
LweI GCATC 3 cut(s) 295, 782, 967
MaeI CTAG 4 cut(s) 81, 308, 464, 1099
MaeII ACGT 3 cut(s) 205, 573, 801
MaeIII GTNAC 6 cut(s) 52, 101, 139, 561, 646, 871
MalI GATC 4 cut(s) 440, 770, 1010, 1025
MboI GATC 4 cut(s) 438, 768, 1008, 1023
MboII GAAGA 4 cut(s) 34, 37, 296, 1080
MfeI CAATTG 1 cut(s) 10
MflI RGATCY 1 cut(s) 1023
MhlI GDGCHC 1 cut(s) 676
MluI ACGCGT 1 cut(s) 558
Mly113I GGCGCC 1 cut(s) 720
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 1 cut(s) 1013
MnlI CCTC 6 cut(s) 146, 238, 337, 356, 734, 1048
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 3 cut(s) 222, 468, 1017
MslI CAYNNNNRTG 1 cut(s) 1073
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 2 cut(s) 201, 294
MunI CAATTG 1 cut(s) 10
MvnI CGCG 2 cut(s) 560, 680
MwoI GCNNNNNNNGC 5 cut(s) 323, 686, 691, 705, 818
NarI GGCGCC 1 cut(s) 720
NdeII GATC 4 cut(s) 438, 768, 1008, 1023
NlaIII CATG 4 cut(s) 259, 838, 891, 1078
NlaIV GGNNCC 2 cut(s) 721, 1036
NmuCI GTSAC 3 cut(s) 101, 139, 871
NspI RCATGY 2 cut(s) 259, 891
NspV TTCGAA 1 cut(s) 408
PaeI GCATGC 1 cut(s) 259
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 269
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 4 cut(s) 342, 604, 1060, 1085
PkrI GCNGC 4 cut(s) 701, 736, 739, 814
PleI GAGTC 1 cut(s) 98
PluTI GGCGCC 1 cut(s) 723
PpsI GAGTC 1 cut(s) 98
PspN4I GGNNCC 2 cut(s) 721, 1036
PspPI GGNCC 2 cut(s) 443, 902
PstNI CAGNNNCTG 1 cut(s) 1027
PsuI RGATCY 1 cut(s) 1023
RsaI GTAC 3 cut(s) 275, 416, 908
RsaNI GTAC 3 cut(s) 274, 415, 907
RseI CAYNNNNRTG 1 cut(s) 1073
SacII CCGCGG 1 cut(s) 681
SalI GTCGAC 2 cut(s) 113, 262
SaqAI TTAA 3 cut(s) 222, 468, 1017
SatI GCNGC 4 cut(s) 700, 735, 738, 813
Sau3AI GATC 4 cut(s) 438, 768, 1008, 1023
Sau96I GGNCC 2 cut(s) 443, 902
SchI GAGTC 1 cut(s) 98
SduI GDGCHC 1 cut(s) 676
SfaNI GCATC 3 cut(s) 295, 782, 967
SfcI CTRYAG 1 cut(s) 249
SfoI GGCGCC 1 cut(s) 721
Sfr303I CCGCGG 1 cut(s) 681
SfuI TTCGAA 1 cut(s) 408
SgrBI CCGCGG 1 cut(s) 681
SinI GGWCC 2 cut(s) 443, 902
SmiMI CAYNNNNRTG 1 cut(s) 1073
SphI GCATGC 1 cut(s) 259
SsiI CCGC 7 cut(s) 284, 330, 678, 680, 700, 738, 752
SspDI GGCGCC 1 cut(s) 719
SspMI CTAG 4 cut(s) 81, 308, 464, 1099
TaaI ACNGT 2 cut(s) 414, 906
TaiI ACGT 3 cut(s) 208, 576, 804
TaqI TCGA 6 cut(s) 114, 177, 263, 408, 878, 938
TauI GCSGC 2 cut(s) 702, 740
TfiI GAWTC 4 cut(s) 342, 604, 1060, 1085
Tru1I TTAA 3 cut(s) 222, 468, 1017
Tru9I TTAA 3 cut(s) 222, 468, 1017
TscAI CASTG 3 cut(s) 151, 247, 361
TseFI GTSAC 3 cut(s) 101, 139, 871
TseI GCWGC 2 cut(s) 734, 812
Tsp45I GTSAC 3 cut(s) 101, 139, 871
TspDTI ATGAA 5 cut(s) 17, 37, 845, 974, 1073
TspGWI ACGGA 2 cut(s) 522, 612
TspRI CASTG 3 cut(s) 151, 247, 361
VpaK11BI GGWCC 2 cut(s) 443, 902
XapI RAATTY 3 cut(s) 360, 384, 940
XceI RCATGY 2 cut(s) 259, 891
XmiI GTMKAC 3 cut(s) 114, 193, 263
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 4 cut(s) 81, 308, 464, 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.