MD04G1234300.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010703
Physical Location & Seq
Forward (+)
31314814 .. 31318169
3356 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1234300.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGCATACCATTTTTGTTTCTTGGGTTGTTCTAGGTCTGCTTGCTGCTTGTGCTGGCCGTCAAGCAGAGGCACGGGCTTTTTTCGTGTTTGGAGATTCGTTGGTGGACAGCGGAAACAACGACTACTTGGCCACCACTGCCAGGGCCGACTCCTACCCTTACGGCATAGATTATCCTACTCACCGACCCACCGGTCGTTTCTCGAATGGCCTTAACATACCAGATCTTATCAGTGAGCAAATTGGCTCAGAACCCACGTTGCCCTACTTGAGCCCACAACTCACTGGACAAAACCTACTTGTTGGTGCCAACTTTGCTTCTGCCGGGATTGGAATTCTCAACGACACTGGAATCCAATTTCTCAACATAATCCGAATCTACAAACAGTTGGAATACTTTCAGCAATACCAGACCAGAGTGAGTGCTTTAGTTGGACCTGAGCAGGCTCAACGACTTGTCAACGAAGCACTTTATCTGATTACCCTCGGAGGCAATGATTTTGTCAACAACTACTACTTGTTGCCCTTCTCTGCTAGATCTCGCCAATTCTCTCTCCCGGACTATGTTGTCTACCTTATCTCCGAGTACCGCAAAGTTCTGGAGAGGCTGTATGAATTAGGAGCTCGTAAGGTTTTGGTGACAGGCACAGGGCCACTAGGGTGTGTTCCTGCAGAGTTGGCTCAGCACAGCACGAACGGCGAATGCGCAGTGGAGCTGCAGCGAGCGGCCTCCTTGTTCAACCCACAACTAGTCGATATGATCAACAGCCTGAATAGCCAATTTGGCTCAGATGCATTTGTTGCTGCAAATGCATATGAAATGCACATGGATTTTGTCTCCGATCCTCAAGCTTATGGATTTGTTACTTCAAAGATAGCTTGCTGTGGCCAAGGACCCTACAATGGGATTGGACTCTGCACATTTGCCTCCAACTTGTGCCCAAACAGAGATTTGTACGCATTTTGGGATCCTTTCCATCCTTCTGAGAAGGCAAACAGAATTATTGTACAGCAAATCCTTACCGGGTCCAACAAGTACATGAATCCAATGAACCTCAGCACTATTTTGGCCTTGGACACTAAGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0042335 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

363

Amino Acids

39.89

Weight (kDa)

5.26

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 338 1.4e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04290 AT5G33370 AT5G33370
fragaria_vesca FvH4_6g01390 FvH4_6g01390
malus_domestica MD04G1234300.v1.1 MD12G1252200.v1.1
prunus_persica Prupe.6G354100_v2.0.a1
pyrus_communis pycom04g20800
rosa_chinensis RchiOBHm_Chr3g0448981 RchiOBHm_Chr3g0449021
rosa_laevigata RLG00000025862
rosa_multiflora Rmu_sc0003116.1_g000011
rosa_roxburghii Rroxscaffold_6G00427140
rosa_rugosa Rorug02G0617400
rosa_samantha Rh3AG015900 Rh3BG016000 Rh3CG015000 Rh3DG016300
rosa_wichuraiana Rw3G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 192, 566
Acc16I TGCGCA 1 cut(s) 706
AccB1I GGYRCC 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 725
AccI GTMKAC 1 cut(s) 570
AciI CCGC 3 cut(s) 111, 589, 725
AclWI GGATC 3 cut(s) 836, 962, 975
AcoI YGGCCR 3 cut(s) 55, 129, 886
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 4 cut(s) 587, 956, 1008, 1037
AfiI CCNNNNNNNGG 3 cut(s) 141, 902, 903
AgeI ACCGGT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 739, 870
AhlI ACTAGT 1 cut(s) 748
AjnI CCWGG 1 cut(s) 140
AleI CACNNNNGTG 1 cut(s) 658
AluBI AGCT 4 cut(s) 623, 715, 851, 878
AluI AGCT 4 cut(s) 623, 715, 851, 878
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 1 cut(s) 841
AlwI GGATC 3 cut(s) 836, 962, 975
AoxI GGCC 8 cut(s) 55, 129, 144, 208, 650, 726, 886, 1068
ApeKI GCWGC 4 cut(s) 44, 715, 718, 803
ApoI RAATTY 1 cut(s) 333
AsiGI ACCGGT 1 cut(s) 191
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 1 cut(s) 707
AspS9I GGNCC 5 cut(s) 144, 434, 650, 893, 1026
AsuC2I CCSGG 3 cut(s) 325, 557, 1024
AsuHPI GGTGA 2 cut(s) 173, 649
AvaII GGWCC 3 cut(s) 434, 893, 1026
BaeGI GKGCMC 1 cut(s) 941
BalI TGGCCA 2 cut(s) 131, 888
BamHI GGATCC 1 cut(s) 967
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 2 cut(s) 275, 625
Bbv12I GWGCWC 1 cut(s) 625
BbvCI CCTCAGC 1 cut(s) 1055
BbvI GCAGC 4 cut(s) 31, 702, 730, 790
BccI CCATC 1 cut(s) 984
BceAI ACGGC 3 cut(s) 42, 178, 712
BciT130I CCWGG 1 cut(s) 142
BclI TGATCA 1 cut(s) 759
BcnI CCSGG 3 cut(s) 325, 557, 1024
BcoDI GTCTC 1 cut(s) 841
BcuI ACTAGT 1 cut(s) 748
BfaI CTAG 5 cut(s) 32, 534, 656, 749, 1087
BfmI CTRYAG 2 cut(s) 669, 716
BglI GCCNNNNNGGC 1 cut(s) 783
BglII AGATCT 2 cut(s) 223, 536
BisI GCNGC 5 cut(s) 45, 716, 719, 726, 804
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 5 cut(s) 46, 717, 720, 727, 805
Bme1390I CCNGG 4 cut(s) 142, 325, 557, 1024
Bme18I GGWCC 3 cut(s) 434, 893, 1026
BmgT120I GGNCC 5 cut(s) 144, 434, 650, 893, 1026
BmiI GGNNCC 4 cut(s) 307, 895, 969, 1027
BmrFI CCNGG 4 cut(s) 142, 325, 557, 1024
BmsI GCATC 1 cut(s) 781
BpmI CTGGAG 1 cut(s) 620
Bpu10I CCTNAGC 2 cut(s) 438, 1055
Bpu1102I GCTNAGC 1 cut(s) 681
BpuEI CTTGAG 2 cut(s) 289, 831
BpuMI CCSGG 3 cut(s) 325, 557, 1024
BsaJI CCNNGG 4 cut(s) 141, 484, 889, 1071
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 4 cut(s) 563, 593, 593, 623
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 902, 903
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 2 cut(s) 289, 352
Bse3DI GCAATG 1 cut(s) 499
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 4 cut(s) 141, 484, 889, 1071
BseGI GGATG 1 cut(s) 976
BseLI CCNNNNNNNGG 3 cut(s) 141, 902, 903
BseMI GCAATG 1 cut(s) 499
BseMII CTCAG 6 cut(s) 261, 429, 695, 801, 975, 1069
BseNI ACTGG 2 cut(s) 289, 352
BseSI GKGCMC 1 cut(s) 941
BseXI GCAGC 4 cut(s) 31, 702, 730, 790
BsgI GTGCAG 1 cut(s) 901
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 8 cut(s) 57, 131, 146, 210, 652, 728, 888, 1070
BshNI GGYRCC 1 cut(s) 305
BshTI ACCGGT 1 cut(s) 191
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 625
BsiSI CCGG 4 cut(s) 192, 324, 557, 1023
BslI CCNNNNNNNGG 3 cut(s) 141, 902, 903
BsmAI GTCTC 1 cut(s) 841
BsmI GAATGC 1 cut(s) 707
BsnI GGCC 8 cut(s) 57, 131, 146, 210, 652, 728, 888, 1070
Bsp1286I GDGCHC 3 cut(s) 275, 625, 941
Bsp1407I TGTACA 1 cut(s) 1006
Bsp143I GATC 5 cut(s) 223, 536, 759, 841, 967
Bsp1720I GCTNAGC 1 cut(s) 681
BspACI CCGC 3 cut(s) 111, 589, 725
BspANI GGCC 8 cut(s) 57, 131, 146, 210, 652, 728, 888, 1070
BspCNI CTCAG 6 cut(s) 260, 430, 694, 800, 976, 1068
BspLI GGNNCC 4 cut(s) 307, 895, 969, 1027
BspMAI CTGCAG 2 cut(s) 673, 720
BspPI GGATC 3 cut(s) 836, 962, 975
BspT107I GGYRCC 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 725
BsrDI GCAATG 1 cut(s) 499
BsrFI RCCGGY 1 cut(s) 191
BsrGI TGTACA 1 cut(s) 1006
BsrI ACTGG 2 cut(s) 289, 352
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 4 cut(s) 141, 484, 889, 1071
BssMI GATC 5 cut(s) 223, 536, 759, 841, 967
BssT1I CCWWGG 2 cut(s) 889, 1071
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 387
BstAPI GCANNNNNTGC 1 cut(s) 800
BstAUI TGTACA 1 cut(s) 1006
BstC8I GCNNGC 5 cut(s) 42, 55, 444, 723, 880
BstDEI CTNAG 7 cut(s) 247, 438, 681, 787, 984, 1055, 1080
BstF5I GGATG 1 cut(s) 976
BstHHI GCGC 1 cut(s) 707
BstKTI GATC 5 cut(s) 226, 539, 762, 844, 970
BstMAI GTCTC 1 cut(s) 841
BstMBI GATC 5 cut(s) 223, 536, 759, 841, 967
BstMCI CGRYCG 1 cut(s) 196
BstMWI GCNNNNNNNGC 9 cut(s) 50, 137, 314, 677, 696, 774, 783, 800, 809
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 4 cut(s) 140, 323, 555, 1022
BstSFI CTRYAG 2 cut(s) 669, 716
BstSLI GKGCMC 1 cut(s) 941
BstV1I GCAGC 4 cut(s) 31, 702, 730, 790
BstX2I RGATCY 3 cut(s) 223, 536, 967
BstYI RGATCY 3 cut(s) 223, 536, 967
BsuRI GGCC 8 cut(s) 57, 131, 146, 210, 652, 728, 888, 1070
BtsCI GGATG 1 cut(s) 976
BtsI GCAGTG 2 cut(s) 135, 714
BtsIMutI CAGTG 5 cut(s) 135, 238, 282, 345, 714
Cac8I GCNNGC 5 cut(s) 42, 55, 444, 723, 880
CfoI GCGC 1 cut(s) 707
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 5 cut(s) 144, 434, 650, 893, 1026
Csp6I GTAC 4 cut(s) 586, 955, 1007, 1036
CspAI ACCGGT 1 cut(s) 191
CviAII CATG 2 cut(s) 826, 1039
CviQI GTAC 4 cut(s) 586, 955, 1007, 1036
DdeI CTNAG 7 cut(s) 247, 438, 681, 787, 984, 1055, 1080
DpnI GATC 5 cut(s) 225, 538, 761, 843, 969
DpnII GATC 5 cut(s) 223, 536, 759, 841, 967
DrdI GACNNNNNNGTC 2 cut(s) 192, 566
DseDI GACNNNNNNGTC 2 cut(s) 192, 566
EaeI YGGCCR 3 cut(s) 55, 129, 886
Ecl136II GAGCTC 1 cut(s) 623
Eco130I CCWWGG 2 cut(s) 889, 1071
Eco24I GRGCYC 2 cut(s) 275, 625
Eco47I GGWCC 3 cut(s) 434, 893, 1026
Eco53kI GAGCTC 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 893
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 2 cut(s) 889, 1071
EcoT22I ATGCAT 3 cut(s) 6, 796, 814
EcoT38I GRGCYC 2 cut(s) 275, 625
ErhI CCWWGG 2 cut(s) 889, 1071
FaeI CATG 2 cut(s) 829, 1042
FatI CATG 2 cut(s) 825, 1038
FauNDI CATATG 1 cut(s) 814
FbaI TGATCA 1 cut(s) 759
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 5 cut(s) 45, 716, 719, 726, 804
FokI GGATG 1 cut(s) 963
FriOI GRGCYC 2 cut(s) 275, 625
Fsp4HI GCNGC 5 cut(s) 45, 716, 719, 726, 804
FspBI CTAG 5 cut(s) 32, 534, 656, 749, 1087
FspI TGCGCA 1 cut(s) 706
GlaI GCGC 1 cut(s) 706
GluI GCNGC 5 cut(s) 45, 716, 719, 726, 804
GsuI CTGGAG 1 cut(s) 620
HaeIII GGCC 8 cut(s) 57, 131, 146, 210, 652, 728, 888, 1070
HapII CCGG 4 cut(s) 192, 324, 557, 1023
HhaI GCGC 1 cut(s) 707
Hin1II CATG 2 cut(s) 829, 1042
Hin6I GCGC 1 cut(s) 705
HinP1I GCGC 1 cut(s) 705
HincII GTYRAC 2 cut(s) 460, 505
HindII GTYRAC 2 cut(s) 460, 505
HindIII AAGCTT 1 cut(s) 849
HinfI GANTC 6 cut(s) 95, 149, 351, 375, 912, 1042
HpaII CCGG 4 cut(s) 192, 324, 557, 1023
HphI GGTGA 2 cut(s) 173, 649
Hpy166II GTNNAC 4 cut(s) 106, 460, 505, 571
Hpy188I TCNGA 8 cut(s) 250, 374, 477, 488, 583, 790, 841, 985
Hpy188III TCNNGA 2 cut(s) 202, 599
Hpy8I GTNNAC 4 cut(s) 106, 460, 505, 571
HpyAV CCTTC 3 cut(s) 535, 982, 990
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 8 cut(s) 4, 671, 718, 794, 806, 812, 823, 918
HpyF10VI GCNNNNNNNGC 9 cut(s) 50, 137, 314, 677, 696, 774, 783, 800, 809
HpyF3I CTNAG 7 cut(s) 247, 438, 681, 787, 984, 1055, 1080
HpySE526I ACGT 1 cut(s) 257
Hsp92II CATG 2 cut(s) 829, 1042
HspAI GCGC 1 cut(s) 705
Ksp22I TGATCA 1 cut(s) 759
Kzo9I GATC 5 cut(s) 223, 536, 759, 841, 967
LmnI GCTCC 2 cut(s) 620, 712
Lsp1109I GCAGC 4 cut(s) 31, 702, 730, 790
LweI GCATC 1 cut(s) 781
MaeI CTAG 5 cut(s) 32, 534, 656, 749, 1087
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 2 cut(s) 637, 862
MalI GATC 5 cut(s) 225, 538, 761, 843, 969
MbiI CCGCTC 1 cut(s) 725
MboI GATC 5 cut(s) 223, 536, 759, 841, 967
MflI RGATCY 3 cut(s) 223, 536, 967
MhlI GDGCHC 3 cut(s) 275, 625, 941
MlsI TGGCCA 2 cut(s) 131, 888
MluCI AATT 7 cut(s) 240, 333, 356, 545, 614, 779, 999
MluNI TGGCCA 2 cut(s) 131, 888
MlyI GAGTC 2 cut(s) 143, 906
MmeI TCCRAC 4 cut(s) 369, 412, 954, 1053
MnlI CCTC 8 cut(s) 61, 482, 494, 597, 739, 855, 937, 1064
Mox20I TGGCCA 2 cut(s) 131, 888
Mph1103I ATGCAT 3 cut(s) 6, 796, 814
MroXI GAANNNNTTC 1 cut(s) 396
MscI TGGCCA 2 cut(s) 131, 888
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 1 cut(s) 658
Msp20I TGGCCA 2 cut(s) 131, 888
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 4 cut(s) 192, 324, 557, 1023
MspR9I CCNGG 4 cut(s) 142, 325, 557, 1024
Mva1269I GAATGC 1 cut(s) 707
MvaI CCWGG 1 cut(s) 142
MwoI GCNNNNNNNGC 9 cut(s) 50, 137, 314, 677, 696, 774, 783, 800, 809
NciI CCSGG 3 cut(s) 325, 557, 1024
NdeI CATATG 1 cut(s) 814
NdeII GATC 5 cut(s) 223, 536, 759, 841, 967
NlaIII CATG 2 cut(s) 829, 1042
NlaIV GGNNCC 4 cut(s) 307, 895, 969, 1027
NmuCI GTSAC 1 cut(s) 637
NsbI TGCGCA 1 cut(s) 706
NsiI ATGCAT 3 cut(s) 6, 796, 814
OliI CACNNNNGTG 1 cut(s) 658
PctI GAATGC 1 cut(s) 707
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 4 cut(s) 95, 351, 375, 1042
PfoI TCCNGGA 1 cut(s) 555
PinAI ACCGGT 1 cut(s) 191
PkrI GCNGC 5 cut(s) 46, 717, 720, 727, 805
PleI GAGTC 2 cut(s) 143, 906
PpsI GAGTC 2 cut(s) 143, 906
PpuMI RGGWCCY 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 625
Psp5II RGGWCCY 1 cut(s) 893
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 4 cut(s) 307, 895, 969, 1027
PspPI GGNCC 5 cut(s) 144, 434, 650, 893, 1026
PspPPI RGGWCCY 1 cut(s) 893
PstI CTGCAG 2 cut(s) 673, 720
PsuI RGATCY 3 cut(s) 223, 536, 967
RsaI GTAC 4 cut(s) 587, 956, 1008, 1037
RsaNI GTAC 4 cut(s) 586, 955, 1007, 1036
RseI CAYNNNNRTG 1 cut(s) 658
SacI GAGCTC 1 cut(s) 625
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 5 cut(s) 45, 716, 719, 726, 804
Sau3AI GATC 5 cut(s) 223, 536, 759, 841, 967
Sau96I GGNCC 5 cut(s) 144, 434, 650, 893, 1026
SchI GAGTC 2 cut(s) 143, 906
ScrFI CCNGG 4 cut(s) 142, 325, 557, 1024
SduI GDGCHC 3 cut(s) 275, 625, 941
SfaNI GCATC 1 cut(s) 781
SfcI CTRYAG 2 cut(s) 669, 716
SinI GGWCC 3 cut(s) 434, 893, 1026
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 2 cut(s) 268, 846
SmoI CTYRAG 2 cut(s) 268, 846
SpeI ACTAGT 1 cut(s) 748
Sse9I AATT 7 cut(s) 240, 333, 356, 545, 614, 779, 999
SsiI CCGC 3 cut(s) 111, 589, 725
SspMI CTAG 5 cut(s) 32, 534, 656, 749, 1087
SstI GAGCTC 1 cut(s) 625
StyD4I CCNGG 4 cut(s) 140, 323, 555, 1022
StyI CCWWGG 2 cut(s) 889, 1071
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 1 cut(s) 260
TaqI TCGA 2 cut(s) 203, 753
TasI AATT 7 cut(s) 240, 333, 356, 545, 614, 779, 999
TatI WGTACW 2 cut(s) 1006, 1035
TauI GCSGC 1 cut(s) 728
TfiI GAWTC 4 cut(s) 95, 351, 375, 1042
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 5 cut(s) 142, 238, 289, 352, 714
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 4 cut(s) 44, 715, 718, 803
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 4 cut(s) 627, 831, 1055, 1064
TspRI CASTG 5 cut(s) 142, 238, 289, 352, 714
VpaK11BI GGWCC 3 cut(s) 434, 893, 1026
XapI RAATTY 1 cut(s) 333
XmiI GTMKAC 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 5 cut(s) 32, 534, 656, 749, 1087
Zsp2I ATGCAT 3 cut(s) 6, 796, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.