Rw3G001190

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010703
Physical Location & Seq
Reverse (-)
962325 .. 965200
2876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001190.1

Sequence Viewer

Length: 1098 bp
ATGCATACAAACACAATGTTGGCTTTTTGGGTTGCTTTGGTCCTGCTGGTTGCTTGTGCTGGTCGTCAAGCCGAGGCACGAGCATTCTTTGTTTTCGGAGATTCGCTGGTGGACAATGGCAACAATGACTACTTGGTCACCACTGCCCGGGCCGACTCGTACCCTTATGGGATCGACTACCCGACTCACATACCAACCGGACGGTTTTCTAATGGCCTCAACATACCAGACCTCATCAGTGAGTCAATTGGGTCAGAACCCACATTGCCATACTTGAGTCCTCAGCTCACTGGACAAAACCTACTTGTTGGTGCCAACTTTGCTTCTGCTGGGATTGGAATCCTCAATGACACTGGAATCCAGTTTCTTAACATAATCAGAATCACCAAGCAACTGGAATACTTCGAACAATACCAGACAAGGGTGAGTGCCCTAATCGGACCTGATCAGACTCAAAGCCTTGTGAACGGAGCCCTGGTCCTTATAACCCTTGGTGGAAATGACTTTGTGAACAACTACTACTTGGTTCCCTACTCTGCTAGGTCTCGCCAATACTCTCTCCCAGACTACGTCGTCTACCTCATCTCTGAGTATCGCAAAATCCTCAACAGGCTGTACGAACTGGGAGCCCGTCAAGTCCTGGTCACAGGCACAGGACCACTAGGCTGTGTCCCCGCCGAGCTTGCCATGCATAGCCGAAACGGGGAGTGCTCCGTGGAGCTCCAACGCGCTGCCTCGCTCTTCAACCCGCAGCTAGTCCAAATGATCAGCTCGCTCAACAGCGATCTCGGGTCCAACGTCTTCATTGCCGCAAACGCCTACCAAATGCACATGGATTTCGTTAGTAACCCCGCTGCCTACGGGTTTGTAACATCAAAGATAGCCTGTTGTGGCCAAGGACCTTACAATGGGATCGGACTATGCACACCCCTTTCGAACCTGTGCCCGAACAGAGACTTGTACGCGTTTTGGGATCCGTTCCATCCTTCGGAGAAGGCTAATAGGATCATTGTGCAGCAAATCCTGACTGGGACCGAAGAGTACATGCACCCCATGAACCTCAGTACTATGTTGGCCTTGGACAACTCTAGGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0042335 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

365

Amino Acids

40.1

Weight (kDa)

5.21

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 340 1.2e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04290 AT5G33370 AT5G33370
fragaria_vesca FvH4_6g01390 FvH4_6g01390
malus_domestica MD04G1234300.v1.1 MD12G1252200.v1.1
prunus_persica Prupe.6G354100_v2.0.a1
pyrus_communis pycom04g20800
rosa_chinensis RchiOBHm_Chr3g0448981 RchiOBHm_Chr3g0449021
rosa_laevigata RLG00000025862
rosa_multiflora Rmu_sc0003116.1_g000011
rosa_roxburghii Rroxscaffold_6G00427140
rosa_rugosa Rorug02G0617400
rosa_samantha Rh3AG015900 Rh3BG016000 Rh3CG015000 Rh3DG016300
rosa_wichuraiana Rw3G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 485
AasI GACNNNNNNGTC 2 cut(s) 134, 572
AccB1I GGYRCC 1 cut(s) 311
AccI GTMKAC 1 cut(s) 576
AccII CGCG 2 cut(s) 729, 965
AciI CCGC 4 cut(s) 675, 749, 810, 852
AclWI GGATC 5 cut(s) 179, 920, 968, 981, 1013
AcoI YGGCCR 1 cut(s) 892
AfaI GTAC 5 cut(s) 161, 617, 962, 1043, 1066
AfiI CCNNNNNNNGG 5 cut(s) 147, 421, 703, 908, 988
AflIII ACRYGT 1 cut(s) 963
AgsI TTSAA 1 cut(s) 745
AjnI CCWGG 2 cut(s) 474, 639
AluBI AGCT 5 cut(s) 286, 682, 721, 754, 771
AluI AGCT 5 cut(s) 286, 682, 721, 754, 771
Alw21I GWGCWC 2 cut(s) 713, 723
Alw26I GTCTC 2 cut(s) 549, 948
AlwI GGATC 5 cut(s) 179, 920, 968, 981, 1013
Ama87I CYCGRG 2 cut(s) 147, 788
AoxI GGCC 4 cut(s) 150, 214, 892, 1074
ApeKI GCWGC 4 cut(s) 731, 751, 854, 1015
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 9 cut(s) 40, 150, 440, 478, 656, 792, 899, 1032, 1092
AsuC2I CCSGG 2 cut(s) 148, 149
AsuHPI GGTGA 3 cut(s) 130, 376, 436
AsuII TTCGAA 2 cut(s) 405, 935
AvaI CYCGRG 2 cut(s) 147, 788
AvaII GGWCC 8 cut(s) 40, 440, 478, 656, 792, 899, 1032, 1092
BaeGI GKGCMC 2 cut(s) 433, 947
BalI TGGCCA 1 cut(s) 894
BamHI GGATCC 1 cut(s) 973
BanI GGYRCC 1 cut(s) 311
BanII GRGCYC 3 cut(s) 475, 631, 723
BauI CACGAG 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 793
Bbv12I GWGCWC 2 cut(s) 713, 723
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 4 cut(s) 718, 763, 841, 1027
BccI CCATC 1 cut(s) 990
BciT130I CCWGG 2 cut(s) 476, 641
BclI TGATCA 2 cut(s) 445, 765
BcnI CCSGG 2 cut(s) 148, 149
BcoDI GTCTC 2 cut(s) 549, 948
BfaI CTAG 5 cut(s) 540, 662, 755, 1089, 1096
BisI GCNGC 5 cut(s) 732, 752, 810, 855, 1016
BlsI GCNGC 5 cut(s) 733, 753, 811, 856, 1017
BmcAI AGTACT 1 cut(s) 1066
Bme1390I CCNGG 4 cut(s) 148, 149, 476, 641
Bme18I GGWCC 8 cut(s) 40, 440, 478, 656, 792, 899, 1032, 1092
BmeT110I CYCGRG 2 cut(s) 147, 788
BmgT120I GGNCC 9 cut(s) 40, 150, 440, 478, 656, 792, 899, 1032, 1092
BmiI GGNNCC 7 cut(s) 313, 472, 528, 628, 793, 975, 1033
BmrFI CCNGG 4 cut(s) 148, 149, 476, 641
BmrI ACTGGG 2 cut(s) 632, 1038
BmuI ACTGGG 2 cut(s) 632, 1038
BpiI GAAGAC 1 cut(s) 793
Bpu10I CCTNAGC 1 cut(s) 282
Bpu14I TTCGAA 2 cut(s) 405, 935
BpuEI CTTGAG 1 cut(s) 295
BpuMI CCSGG 2 cut(s) 148, 149
BsaBI GATNNNNATC 1 cut(s) 338
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 7 cut(s) 72, 147, 474, 490, 714, 895, 1077
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 5 cut(s) 147, 421, 703, 908, 988
Bse1I ACTGG 6 cut(s) 295, 358, 361, 399, 627, 1033
Bse3DI GCAATG 2 cut(s) 263, 804
Bse8I GATNNNNATC 1 cut(s) 338
BseBI CCWGG 2 cut(s) 476, 641
BseDI CCNNGG 7 cut(s) 72, 147, 474, 490, 714, 895, 1077
BseGI GGATG 1 cut(s) 982
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 5 cut(s) 147, 421, 703, 908, 988
BseMI GCAATG 2 cut(s) 263, 804
BseMII CTCAG 3 cut(s) 296, 579, 1075
BseNI ACTGG 6 cut(s) 295, 358, 361, 399, 627, 1033
BseSI GKGCMC 2 cut(s) 433, 947
BseXI GCAGC 4 cut(s) 718, 763, 841, 1027
BseYI CCCAGC 1 cut(s) 329
BsgI GTGCAG 1 cut(s) 1034
Bsh1236I CGCG 2 cut(s) 729, 965
BshFI GGCC 4 cut(s) 152, 216, 894, 1076
BshNI GGYRCC 1 cut(s) 311
BsiHKAI GWGCWC 2 cut(s) 713, 723
BsiHKCI CYCGRG 2 cut(s) 147, 788
BsiSI CCGG 2 cut(s) 148, 198
BslFI GGGAC 2 cut(s) 656, 1045
BslI CCNNNNNNNGG 5 cut(s) 147, 421, 703, 908, 988
BsmAI GTCTC 2 cut(s) 549, 948
BsmFI GGGAC 2 cut(s) 656, 1045
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 4 cut(s) 152, 216, 894, 1076
Bso31I GGTCTC 1 cut(s) 549
BsoBI CYCGRG 2 cut(s) 147, 788
Bsp119I TTCGAA 2 cut(s) 405, 935
Bsp1286I GDGCHC 6 cut(s) 433, 475, 631, 713, 723, 947
Bsp143I GATC 7 cut(s) 171, 445, 765, 784, 912, 973, 1005
BspACI CCGC 4 cut(s) 675, 749, 810, 852
BspANI GGCC 4 cut(s) 152, 216, 894, 1076
BspCNI CTCAG 3 cut(s) 295, 580, 1074
BspFNI CGCG 2 cut(s) 729, 965
BspLI GGNNCC 7 cut(s) 313, 472, 528, 628, 793, 975, 1033
BspPI GGATC 5 cut(s) 179, 920, 968, 981, 1013
BspQI GCTCTTC 1 cut(s) 746
BspT104I TTCGAA 2 cut(s) 405, 935
BspT107I GGYRCC 1 cut(s) 311
BspTNI GGTCTC 1 cut(s) 549
BsrDI GCAATG 2 cut(s) 263, 804
BsrI ACTGG 6 cut(s) 295, 358, 361, 399, 627, 1033
BssECI CCNNGG 7 cut(s) 72, 147, 474, 490, 714, 895, 1077
BssMI GATC 7 cut(s) 171, 445, 765, 784, 912, 973, 1005
BssSI CACGAG 1 cut(s) 78
BssT1I CCWWGG 3 cut(s) 490, 895, 1077
Bst2BI CACGAG 1 cut(s) 78
Bst2UI CCWGG 2 cut(s) 476, 641
Bst4CI ACNGT 1 cut(s) 204
Bst6I CTCTTC 2 cut(s) 746, 1032
BstBI TTCGAA 2 cut(s) 405, 935
BstC8I GCNNGC 2 cut(s) 684, 773
BstDEI CTNAG 3 cut(s) 282, 588, 1061
BstDSI CCRYGG 1 cut(s) 714
BstEII GGTNACC 1 cut(s) 136
BstF5I GGATG 1 cut(s) 982
BstFNI CGCG 2 cut(s) 729, 965
BstHHI GCGC 1 cut(s) 731
BstKTI GATC 7 cut(s) 174, 448, 768, 787, 915, 976, 1008
BstMAI GTCTC 2 cut(s) 549, 948
BstMBI GATC 7 cut(s) 171, 445, 765, 784, 912, 973, 1005
BstMWI GCNNNNNNNGC 4 cut(s) 320, 683, 688, 815
BstNI CCWGG 2 cut(s) 476, 641
BstNSI RCATGY 1 cut(s) 1048
BstPI GGTNACC 1 cut(s) 136
BstSCI CCNGG 4 cut(s) 146, 147, 474, 639
BstSLI GKGCMC 2 cut(s) 433, 947
BstUI CGCG 2 cut(s) 729, 965
BstV1I GCAGC 4 cut(s) 718, 763, 841, 1027
BstV2I GAAGAC 1 cut(s) 793
BstX2I RGATCY 1 cut(s) 973
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 1 cut(s) 973
BsuRI GGCC 4 cut(s) 152, 216, 894, 1076
BtgI CCRYGG 1 cut(s) 714
BtsCI GGATG 1 cut(s) 982
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 4 cut(s) 141, 244, 288, 351
Cac8I GCNNGC 2 cut(s) 684, 773
CfoI GCGC 1 cut(s) 731
Cfr13I GGNCC 9 cut(s) 40, 150, 440, 478, 656, 792, 899, 1032, 1092
Cfr9I CCCGGG 1 cut(s) 147
Csp6I GTAC 5 cut(s) 160, 616, 961, 1042, 1065
CviAII CATG 4 cut(s) 688, 832, 1045, 1054
CviQI GTAC 5 cut(s) 160, 616, 961, 1042, 1065
DdeI CTNAG 3 cut(s) 282, 588, 1061
DpnI GATC 7 cut(s) 173, 447, 767, 786, 914, 975, 1007
DpnII GATC 7 cut(s) 171, 445, 765, 784, 912, 973, 1005
DrdI GACNNNNNNGTC 2 cut(s) 134, 572
DseDI GACNNNNNNGTC 2 cut(s) 134, 572
EaeI YGGCCR 1 cut(s) 892
Eam1104I CTCTTC 2 cut(s) 746, 1032
EarI CTCTTC 2 cut(s) 746, 1032
Ecl136II GAGCTC 1 cut(s) 721
Eco130I CCWWGG 3 cut(s) 490, 895, 1077
Eco24I GRGCYC 3 cut(s) 475, 631, 723
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 8 cut(s) 40, 440, 478, 656, 792, 899, 1032, 1092
Eco53kI GAGCTC 1 cut(s) 721
Eco88I CYCGRG 2 cut(s) 147, 788
Eco91I GGTNACC 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 721
EcoO109I RGGNCCY 2 cut(s) 899, 1092
EcoO65I GGTNACC 1 cut(s) 136
EcoRII CCWGG 2 cut(s) 474, 639
EcoT14I CCWWGG 3 cut(s) 490, 895, 1077
EcoT22I ATGCAT 2 cut(s) 6, 693
EcoT38I GRGCYC 3 cut(s) 475, 631, 723
ErhI CCWWGG 3 cut(s) 490, 895, 1077
FaeI CATG 4 cut(s) 691, 835, 1048, 1057
FaqI GGGAC 2 cut(s) 656, 1045
FatI CATG 4 cut(s) 687, 831, 1044, 1053
FauI CCCGC 3 cut(s) 682, 756, 859
FbaI TGATCA 2 cut(s) 445, 765
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 5 cut(s) 732, 752, 810, 855, 1016
FokI GGATG 1 cut(s) 969
FriOI GRGCYC 3 cut(s) 475, 631, 723
Fsp4HI GCNGC 5 cut(s) 732, 752, 810, 855, 1016
FspBI CTAG 5 cut(s) 540, 662, 755, 1089, 1096
GlaI GCGC 1 cut(s) 730
GluI GCNGC 5 cut(s) 732, 752, 810, 855, 1016
GsaI CCCAGC 1 cut(s) 333
HaeIII GGCC 4 cut(s) 152, 216, 894, 1076
HapII CCGG 2 cut(s) 148, 198
HhaI GCGC 1 cut(s) 731
Hin1II CATG 4 cut(s) 691, 835, 1048, 1057
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HinfI GANTC 9 cut(s) 101, 155, 184, 242, 277, 339, 357, 381, 451
HpaII CCGG 2 cut(s) 148, 198
HphI GGTGA 3 cut(s) 130, 376, 436
Hpy166II GTNNAC 4 cut(s) 112, 466, 511, 577
Hpy188I TCNGA 8 cut(s) 98, 256, 380, 440, 450, 589, 917, 991
Hpy188III TCNNGA 1 cut(s) 1024
Hpy8I GTNNAC 4 cut(s) 112, 466, 511, 577
Hpy99I CGWCG 1 cut(s) 575
HpyAV CCTTC 2 cut(s) 988, 996
HpyCH4III ACNGT 1 cut(s) 204
HpyCH4IV ACGT 2 cut(s) 570, 798
HpyCH4V TGCA 6 cut(s) 4, 691, 829, 924, 1015, 1048
HpyF10VI GCNNNNNNNGC 4 cut(s) 320, 683, 688, 815
HpyF3I CTNAG 3 cut(s) 282, 588, 1061
HpySE526I ACGT 2 cut(s) 570, 798
Hsp92II CATG 4 cut(s) 691, 835, 1048, 1057
HspAI GCGC 1 cut(s) 729
Ksp22I TGATCA 2 cut(s) 445, 765
Kzo9I GATC 7 cut(s) 171, 445, 765, 784, 912, 973, 1005
LguI GCTCTTC 1 cut(s) 746
LmnI GCTCC 5 cut(s) 470, 626, 716, 718, 726
Lsp1109I GCAGC 4 cut(s) 718, 763, 841, 1027
MaeI CTAG 5 cut(s) 540, 662, 755, 1089, 1096
MaeII ACGT 2 cut(s) 570, 798
MaeIII GTNAC 4 cut(s) 136, 643, 845, 868
MalI GATC 7 cut(s) 173, 447, 767, 786, 914, 975, 1007
MboI GATC 7 cut(s) 171, 445, 765, 784, 912, 973, 1005
MboII GAAGA 3 cut(s) 733, 793, 1049
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 1 cut(s) 973
MhlI GDGCHC 6 cut(s) 433, 475, 631, 713, 723, 947
MlsI TGGCCA 1 cut(s) 894
MluCI AATT 1 cut(s) 246
MluI ACGCGT 1 cut(s) 963
MluNI TGGCCA 1 cut(s) 894
MlyI GAGTC 5 cut(s) 149, 178, 251, 286, 445
MmeI TCCRAC 2 cut(s) 748, 819
MnlI CCTC 9 cut(s) 67, 227, 242, 291, 353, 590, 614, 745, 1070
Mox20I TGGCCA 1 cut(s) 894
Mph1103I ATGCAT 2 cut(s) 6, 693
MscI TGGCCA 1 cut(s) 894
MseI TTAA 1 cut(s) 369
Msp20I TGGCCA 1 cut(s) 894
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 2 cut(s) 148, 198
MspR9I CCNGG 4 cut(s) 148, 149, 476, 641
MunI CAATTG 1 cut(s) 246
Mva1269I GAATGC 1 cut(s) 83
MvaI CCWGG 2 cut(s) 476, 641
MvnI CGCG 2 cut(s) 729, 965
MwoI GCNNNNNNNGC 4 cut(s) 320, 683, 688, 815
NciI CCSGG 2 cut(s) 148, 149
NdeII GATC 7 cut(s) 171, 445, 765, 784, 912, 973, 1005
NlaIII CATG 4 cut(s) 691, 835, 1048, 1057
NlaIV GGNNCC 7 cut(s) 313, 472, 528, 628, 793, 975, 1033
NmeAIII GCCGAG 2 cut(s) 97, 703
NmuCI GTSAC 2 cut(s) 136, 643
NsiI ATGCAT 2 cut(s) 6, 693
NspI RCATGY 1 cut(s) 1048
NspV TTCGAA 2 cut(s) 405, 935
PciSI GCTCTTC 1 cut(s) 746
PcsI WCGNNNNNNNCGW 1 cut(s) 795
PctI GAATGC 1 cut(s) 83
PfeI GAWTC 4 cut(s) 101, 339, 357, 381
PflFI GACNNNGTC 1 cut(s) 569
PkrI GCNGC 5 cut(s) 733, 753, 811, 856, 1017
PleI GAGTC 5 cut(s) 149, 178, 250, 285, 445
PpsI GAGTC 5 cut(s) 149, 178, 250, 285, 445
PpuMI RGGWCCY 2 cut(s) 899, 1092
PsiI TTATAA 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 723
Psp5II RGGWCCY 2 cut(s) 899, 1092
Psp6I CCWGG 2 cut(s) 474, 639
PspEI GGTNACC 1 cut(s) 136
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 2 cut(s) 474, 639
PspN4I GGNNCC 7 cut(s) 313, 472, 528, 628, 793, 975, 1033
PspPI GGNCC 9 cut(s) 40, 150, 440, 478, 656, 792, 899, 1032, 1092
PspPPI RGGWCCY 2 cut(s) 899, 1092
PsrI GAACNNNNNNTAC 2 cut(s) 503, 535
PsuI RGATCY 1 cut(s) 973
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 5 cut(s) 161, 617, 962, 1043, 1066
RsaNI GTAC 5 cut(s) 160, 616, 961, 1042, 1065
SacI GAGCTC 1 cut(s) 723
SapI GCTCTTC 1 cut(s) 746
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 5 cut(s) 732, 752, 810, 855, 1016
Sau3AI GATC 7 cut(s) 171, 445, 765, 784, 912, 973, 1005
Sau96I GGNCC 9 cut(s) 40, 150, 440, 478, 656, 792, 899, 1032, 1092
ScaI AGTACT 1 cut(s) 1066
SchI GAGTC 5 cut(s) 149, 178, 251, 286, 445
ScrFI CCNGG 4 cut(s) 148, 149, 476, 641
SduI GDGCHC 6 cut(s) 433, 475, 631, 713, 723, 947
SfuI TTCGAA 2 cut(s) 405, 935
SinI GGWCC 8 cut(s) 40, 440, 478, 656, 792, 899, 1032, 1092
SmaI CCCGGG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
SrfI GCCCGGGC 1 cut(s) 149
Sse9I AATT 1 cut(s) 246
SsiI CCGC 4 cut(s) 675, 749, 810, 852
SspMI CTAG 5 cut(s) 540, 662, 755, 1089, 1096
SstI GAGCTC 1 cut(s) 723
StyD4I CCNGG 4 cut(s) 146, 147, 474, 639
StyI CCWWGG 3 cut(s) 490, 895, 1077
TaaI ACNGT 1 cut(s) 204
TaiI ACGT 2 cut(s) 573, 801
TaqI TCGA 3 cut(s) 174, 405, 935
TaqII GACCGA 1 cut(s) 1049
TasI AATT 1 cut(s) 246
TatI WGTACW 2 cut(s) 1041, 1064
TauI GCSGC 1 cut(s) 812
TfiI GAWTC 4 cut(s) 101, 339, 357, 381
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 4 cut(s) 148, 244, 295, 358
TseFI GTSAC 2 cut(s) 136, 643
TseI GCWGC 4 cut(s) 731, 751, 854, 1015
Tsp45I GTSAC 2 cut(s) 136, 643
TspDTI ATGAA 2 cut(s) 793, 1070
TspGWI ACGGA 3 cut(s) 483, 703, 966
TspMI CCCGGG 1 cut(s) 147
TspRI CASTG 4 cut(s) 148, 244, 295, 358
Tth111I GACNNNGTC 1 cut(s) 569
VpaK11BI GGWCC 8 cut(s) 40, 440, 478, 656, 792, 899, 1032, 1092
XceI RCATGY 1 cut(s) 1048
XmaI CCCGGG 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 576
XspI CTAG 5 cut(s) 540, 662, 755, 1089, 1096
ZrmI AGTACT 1 cut(s) 1066
Zsp2I ATGCAT 2 cut(s) 6, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.