RchiOBHm_Chr3g0449021

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0010703
Physical Location & Seq
Reverse (-)
982631 .. 984298
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41642

Sequence Viewer

Length: 720 bp
ATGCATACAAACACAATGTTGGCTTTTTGGGTTGCTTTGGTCCTGCTGGTTGCTTGTGCTGGTCGTCAAGCCGAGGCACGAGCATTCTTTGTTTTCGGAGATTCGCTGGTGGACAATGGCAACAATGACTACTTGGTCACCACTGCCCGGGCCGACTCGTACCCTTATGGGATCGACTACCCGACTCACATACCAACCGGACGGTTTTCTAATGGCCTCAACATACCAGACCTCATCAGTGAGTCAATTGGGTCAGAACCCACATTGCCATACTTGAGTCCTCACCTCACTGGACAAAACCTACTTGTTGGTGCCAACTTTGCTTCTGCTGGGATTGGAATCCTCAATGACACTGGAATCCAGTTTCTTAACATAATCAGAATCACCAAGCAACTGGAATACTTCGAACAATACCAGACAAGGGTGAGTGCCCTAATCGGACCTGATCAGACTCAAAGCCTTGTGAACGGAGCCCTGGTCCTTATAACCCTTGGTGGAAATGACTTTGTGAACAACTACTACTTGGTTCCCTACTCTGCTAGGTCTCGCCAATACTCTCTCCCAGACTACGTCGTCTACCTCATCTCTGAGTATCGCAAAATCCTCAACGAATCGATTCCTGATAAGAAGGTGGGATCTACATCTAGGTCTATTCCTTCATGTGTTAGTAAATCCATGAATTTTTTTACCCAGAATCATTCCAATTCCTTTATGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0042335 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

239

Amino Acids

26.4

Weight (kDa)

5.75

Isoelectric Point (pI)

29.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 214 2.9e-14 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04290 AT5G33370 AT5G33370
fragaria_vesca FvH4_6g01390 FvH4_6g01390
malus_domestica MD04G1234300.v1.1 MD12G1252200.v1.1
prunus_persica Prupe.6G354100_v2.0.a1
pyrus_communis pycom04g20800
rosa_chinensis RchiOBHm_Chr3g0448981 RchiOBHm_Chr3g0449021
rosa_laevigata RLG00000025862
rosa_multiflora Rmu_sc0003116.1_g000011
rosa_roxburghii Rroxscaffold_6G00427140
rosa_rugosa Rorug02G0617400
rosa_samantha Rh3AG015900 Rh3BG016000 Rh3CG015000 Rh3DG016300
rosa_wichuraiana Rw3G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 485
AasI GACNNNNNNGTC 2 cut(s) 134, 572
AccB1I GGYRCC 1 cut(s) 311
AccI GTMKAC 1 cut(s) 576
AclWI GGATC 2 cut(s) 179, 643
AcsI RAATTY 1 cut(s) 679
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 2 cut(s) 147, 421
AjnI CCWGG 1 cut(s) 474
Alw26I GTCTC 1 cut(s) 549
AlwI GGATC 2 cut(s) 179, 643
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 2 cut(s) 150, 214
ApoI RAATTY 1 cut(s) 679
Asp700I GAANNNNTTC 1 cut(s) 615
AspS9I GGNCC 4 cut(s) 40, 150, 440, 478
AsuC2I CCSGG 2 cut(s) 148, 149
AsuHPI GGTGA 4 cut(s) 130, 275, 376, 436
AsuII TTCGAA 1 cut(s) 405
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 3 cut(s) 40, 440, 478
BaeGI GKGCMC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 311
BanII GRGCYC 1 cut(s) 475
BauI CACGAG 1 cut(s) 78
BciT130I CCWGG 1 cut(s) 476
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 2 cut(s) 148, 149
BcoDI GTCTC 1 cut(s) 549
BfaI CTAG 2 cut(s) 540, 645
Bme1390I CCNGG 3 cut(s) 148, 149, 476
Bme18I GGWCC 3 cut(s) 40, 440, 478
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 4 cut(s) 40, 150, 440, 478
BmiI GGNNCC 3 cut(s) 313, 472, 528
BmrFI CCNGG 3 cut(s) 148, 149, 476
Bpu14I TTCGAA 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 295
BpuMI CCSGG 2 cut(s) 148, 149
Bsa29I ATCGAT 1 cut(s) 614
BsaBI GATNNNNATC 2 cut(s) 338, 640
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 4 cut(s) 72, 147, 474, 490
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 421
Bse1I ACTGG 4 cut(s) 295, 358, 361, 399
Bse3DI GCAATG 1 cut(s) 263
Bse8I GATNNNNATC 2 cut(s) 338, 640
BseBI CCWGG 1 cut(s) 476
BseCI ATCGAT 1 cut(s) 614
BseDI CCNNGG 4 cut(s) 72, 147, 474, 490
BseJI GATNNNNATC 2 cut(s) 338, 640
BseLI CCNNNNNNNGG 2 cut(s) 147, 421
BseMI GCAATG 1 cut(s) 263
BseMII CTCAG 1 cut(s) 579
BseNI ACTGG 4 cut(s) 295, 358, 361, 399
BseSI GKGCMC 1 cut(s) 433
BseYI CCCAGC 1 cut(s) 329
BshFI GGCC 2 cut(s) 152, 216
BshNI GGYRCC 1 cut(s) 311
BshVI ATCGAT 1 cut(s) 614
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 2 cut(s) 148, 198
BslI CCNNNNNNNGG 2 cut(s) 147, 421
BsmAI GTCTC 1 cut(s) 549
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 2 cut(s) 152, 216
Bso31I GGTCTC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 147
Bsp119I TTCGAA 1 cut(s) 405
Bsp1286I GDGCHC 2 cut(s) 433, 475
Bsp143I GATC 3 cut(s) 171, 445, 635
BspANI GGCC 2 cut(s) 152, 216
BspCNI CTCAG 1 cut(s) 580
BspDI ATCGAT 1 cut(s) 614
BspLI GGNNCC 3 cut(s) 313, 472, 528
BspPI GGATC 2 cut(s) 179, 643
BspT104I TTCGAA 1 cut(s) 405
BspT107I GGYRCC 1 cut(s) 311
BspTNI GGTCTC 1 cut(s) 549
BsrDI GCAATG 1 cut(s) 263
BsrI ACTGG 4 cut(s) 295, 358, 361, 399
BssECI CCNNGG 4 cut(s) 72, 147, 474, 490
BssMI GATC 3 cut(s) 171, 445, 635
BssSI CACGAG 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 490
Bst2BI CACGAG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 405
BstDEI CTNAG 1 cut(s) 588
BstEII GGTNACC 1 cut(s) 136
BstKTI GATC 3 cut(s) 174, 448, 638
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 3 cut(s) 171, 445, 635
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstNI CCWGG 1 cut(s) 476
BstPI GGTNACC 1 cut(s) 136
BstSCI CCNGG 3 cut(s) 146, 147, 474
BstSLI GKGCMC 1 cut(s) 433
BstX2I RGATCY 1 cut(s) 635
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 1 cut(s) 635
Bsu15I ATCGAT 1 cut(s) 614
BsuRI GGCC 2 cut(s) 152, 216
BsuTUI ATCGAT 1 cut(s) 614
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 4 cut(s) 141, 244, 288, 351
Cfr13I GGNCC 4 cut(s) 40, 150, 440, 478
Cfr9I CCCGGG 1 cut(s) 147
ClaI ATCGAT 1 cut(s) 614
Csp6I GTAC 1 cut(s) 160
CviAII CATG 2 cut(s) 660, 676
CviJI RGCY 6 cut(s) 23, 71, 152, 216, 459, 473
CviKI_1 RGCY 6 cut(s) 23, 71, 152, 216, 459, 473
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 588
DpnI GATC 3 cut(s) 173, 447, 637
DpnII GATC 3 cut(s) 171, 445, 635
DrdI GACNNNNNNGTC 2 cut(s) 134, 572
DseDI GACNNNNNNGTC 2 cut(s) 134, 572
Eco130I CCWWGG 1 cut(s) 490
Eco24I GRGCYC 1 cut(s) 475
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 3 cut(s) 40, 440, 478
Eco88I CYCGRG 1 cut(s) 147
Eco91I GGTNACC 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 490
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 475
ErhI CCWWGG 1 cut(s) 490
FaeI CATG 2 cut(s) 663, 679
FatI CATG 2 cut(s) 659, 675
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 1 cut(s) 576
FriOI GRGCYC 1 cut(s) 475
FspBI CTAG 2 cut(s) 540, 645
GsaI CCCAGC 1 cut(s) 333
HaeIII GGCC 2 cut(s) 152, 216
HapII CCGG 2 cut(s) 148, 198
Hin1II CATG 2 cut(s) 663, 679
HpaII CCGG 2 cut(s) 148, 198
HphI GGTGA 4 cut(s) 130, 275, 376, 436
Hpy166II GTNNAC 4 cut(s) 112, 466, 511, 577
Hpy188I TCNGA 6 cut(s) 98, 256, 380, 440, 450, 589
Hpy188III TCNNGA 1 cut(s) 620
Hpy8I GTNNAC 4 cut(s) 112, 466, 511, 577
Hpy99I CGWCG 1 cut(s) 575
HpyAV CCTTC 2 cut(s) 622, 666
HpyCH4III ACNGT 1 cut(s) 204
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpyF3I CTNAG 1 cut(s) 588
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 2 cut(s) 663, 679
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 3 cut(s) 171, 445, 635
LmnI GCTCC 1 cut(s) 470
MaeI CTAG 2 cut(s) 540, 645
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 1 cut(s) 136
MalI GATC 3 cut(s) 173, 447, 637
MboI GATC 3 cut(s) 171, 445, 635
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 1 cut(s) 635
MhlI GDGCHC 2 cut(s) 433, 475
MluCI AATT 3 cut(s) 246, 679, 703
MlyI GAGTC 5 cut(s) 149, 178, 251, 286, 445
MnlI CCTC 8 cut(s) 67, 227, 242, 291, 296, 353, 590, 614
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 2 cut(s) 369, 718
MspI CCGG 2 cut(s) 148, 198
MspR9I CCNGG 3 cut(s) 148, 149, 476
MunI CAATTG 1 cut(s) 246
Mva1269I GAATGC 1 cut(s) 83
MvaI CCWGG 1 cut(s) 476
MwoI GCNNNNNNNGC 1 cut(s) 320
NciI CCSGG 2 cut(s) 148, 149
NdeII GATC 3 cut(s) 171, 445, 635
NlaIII CATG 2 cut(s) 663, 679
NlaIV GGNNCC 3 cut(s) 313, 472, 528
NmeAIII GCCGAG 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 136
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 405
PctI GAATGC 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 7 cut(s) 101, 339, 357, 381, 611, 616, 694
PflFI GACNNNGTC 1 cut(s) 569
PleI GAGTC 5 cut(s) 149, 178, 250, 285, 445
PpsI GAGTC 5 cut(s) 149, 178, 250, 285, 445
PsiI TTATAA 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 474
PspEI GGTNACC 1 cut(s) 136
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 3 cut(s) 313, 472, 528
PspPI GGNCC 4 cut(s) 40, 150, 440, 478
PsrI GAACNNNNNNTAC 2 cut(s) 503, 535
PsuI RGATCY 1 cut(s) 635
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SaqAI TTAA 2 cut(s) 369, 718
Sau3AI GATC 3 cut(s) 171, 445, 635
Sau96I GGNCC 4 cut(s) 40, 150, 440, 478
SchI GAGTC 5 cut(s) 149, 178, 251, 286, 445
ScrFI CCNGG 3 cut(s) 148, 149, 476
SduI GDGCHC 2 cut(s) 433, 475
SetI ASST 9 cut(s) 234, 288, 303, 445, 545, 573, 582, 633, 650
SfuI TTCGAA 1 cut(s) 405
SinI GGWCC 3 cut(s) 40, 440, 478
SmaI CCCGGG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
SrfI GCCCGGGC 1 cut(s) 149
Sse9I AATT 3 cut(s) 246, 679, 703
SspMI CTAG 2 cut(s) 540, 645
StyD4I CCNGG 3 cut(s) 146, 147, 474
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 1 cut(s) 204
TaiI ACGT 1 cut(s) 573
TaqI TCGA 3 cut(s) 174, 405, 614
TasI AATT 3 cut(s) 246, 679, 703
TfiI GAWTC 7 cut(s) 101, 339, 357, 381, 611, 616, 694
Tru1I TTAA 2 cut(s) 369, 718
Tru9I TTAA 2 cut(s) 369, 718
TscAI CASTG 4 cut(s) 148, 244, 295, 358
TseFI GTSAC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 2 cut(s) 648, 692
TspGWI ACGGA 1 cut(s) 483
TspMI CCCGGG 1 cut(s) 147
TspRI CASTG 4 cut(s) 148, 244, 295, 358
Tth111I GACNNNGTC 1 cut(s) 569
VpaK11BI GGWCC 3 cut(s) 40, 440, 478
XapI RAATTY 1 cut(s) 679
XmaI CCCGGG 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 576
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 2 cut(s) 540, 645
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.