Rh3CG015000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0010703
Physical Location & Seq
Reverse (-)
997163 .. 1000322
3160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG015000.1

Sequence Viewer

Length: 771 bp
ATGACTAAATATATATGTGTGTGCTACATCATTTGGCAGCTTAACATAATCAGAATCACCAAGCAACTGGAGTACTTCGAACAATACCAGACAAGGGTGAGTGCCCTAATCGGACCTGATCAGACTCAAAGCCTTGTGAACGGAGCCCTGGTCCTTATAACCCTTGGTGGAAATGACTTTGTGAACAACTACTACTTGGTTCCCTACTCTGCTAGGTCTCGCCAATACTCTCTCCCAGACTACGTCGTCTACCTCATCTCTGAGTATCGCAAAATCCTCAACAGGCTGTACGAACTGGGAGCCCGTCAAGTCCTGGTCACAGGCACAGGACCACTAGGCTGTGTCCCCGCCGAGCTTGCCATGCATAGCCGAAACGGGGAGTGCTCCGTGGAGCTCCAACGCGCTGCCTCGCTCTTCAACCCGCAGCTAGTCCAAATGATCAGCTCGCTCAACAGCGATCTCGGGTCCAACGTCTTCATTGCCGCAAACGCCTACCAAATGCACATGGATTTCGTTAGTAACCCCGGTGCCTACGGGTTTGTAACATCAAAGATAGCCTGTTGTGGCCAAGGACCTTACAATGGGATCGGACTATGCACACCCCTTTCGAACCTGTGCCCGAACCGAGACTTATACGCGTTTTGGGATCCGTTCCATCCTTCGGAGAAGGCTAATAGGATCATTGTGCAGCAAATCCTGACCGGGACCGAAGAGTACATGCACCCCATGAACCTCAGTACTATGTTGGCCTTGGACAACTCGAGGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0042335 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

256

Amino Acids

28.7

Weight (kDa)

6.41

Isoelectric Point (pI)

37.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 21 - 231 5.8e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04290 AT5G33370 AT5G33370
fragaria_vesca FvH4_6g01390 FvH4_6g01390
malus_domestica MD04G1234300.v1.1 MD12G1252200.v1.1
prunus_persica Prupe.6G354100_v2.0.a1
pyrus_communis pycom04g20800
rosa_chinensis RchiOBHm_Chr3g0448981 RchiOBHm_Chr3g0449021
rosa_laevigata RLG00000025862
rosa_multiflora Rmu_sc0003116.1_g000011
rosa_roxburghii Rroxscaffold_6G00427140
rosa_rugosa Rorug02G0617400
rosa_samantha Rh3AG015900 Rh3BG016000 Rh3CG015000 Rh3DG016300
rosa_wichuraiana Rw3G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 158
AasI GACNNNNNNGTC 1 cut(s) 245
AccB1I GGYRCC 1 cut(s) 527
AccI GTMKAC 1 cut(s) 249
AccII CGCG 2 cut(s) 402, 638
AciI CCGC 3 cut(s) 348, 422, 483
AclWI GGATC 4 cut(s) 593, 641, 654, 686
AcoI YGGCCR 1 cut(s) 565
AfaI GTAC 4 cut(s) 74, 290, 716, 739
AfiI CCNNNNNNNGG 4 cut(s) 94, 376, 581, 661
AflIII ACRYGT 1 cut(s) 636
AgsI TTSAA 1 cut(s) 418
AjnI CCWGG 2 cut(s) 147, 312
AluBI AGCT 5 cut(s) 40, 355, 394, 427, 444
AluI AGCT 5 cut(s) 40, 355, 394, 427, 444
Alw21I GWGCWC 2 cut(s) 386, 396
Alw26I GTCTC 2 cut(s) 222, 621
AlwI GGATC 4 cut(s) 593, 641, 654, 686
Ama87I CYCGRG 2 cut(s) 461, 760
AoxI GGCC 2 cut(s) 565, 747
ApeKI GCWGC 4 cut(s) 37, 404, 424, 688
AspLEI GCGC 1 cut(s) 404
AspS9I GGNCC 7 cut(s) 113, 151, 329, 465, 572, 705, 765
AsuC2I CCSGG 2 cut(s) 525, 703
AsuHPI GGTGA 2 cut(s) 49, 109
AsuII TTCGAA 2 cut(s) 78, 608
AvaI CYCGRG 2 cut(s) 461, 760
AvaII GGWCC 7 cut(s) 113, 151, 329, 465, 572, 705, 765
BaeGI GKGCMC 2 cut(s) 106, 620
BalI TGGCCA 1 cut(s) 567
BamHI GGATCC 1 cut(s) 646
BanI GGYRCC 1 cut(s) 527
BanII GRGCYC 3 cut(s) 148, 304, 396
BbsI GAAGAC 1 cut(s) 466
Bbv12I GWGCWC 2 cut(s) 386, 396
BbvI GCAGC 4 cut(s) 49, 391, 436, 700
BccI CCATC 1 cut(s) 663
BciT130I CCWGG 2 cut(s) 149, 314
BclI TGATCA 2 cut(s) 118, 438
BcnI CCSGG 2 cut(s) 525, 703
BcoDI GTCTC 2 cut(s) 222, 621
BfaI CTAG 4 cut(s) 213, 335, 428, 769
BisI GCNGC 5 cut(s) 38, 405, 425, 483, 689
BlsI GCNGC 5 cut(s) 39, 406, 426, 484, 690
BmcAI AGTACT 2 cut(s) 74, 739
Bme1390I CCNGG 4 cut(s) 149, 314, 525, 703
Bme18I GGWCC 7 cut(s) 113, 151, 329, 465, 572, 705, 765
BmeT110I CYCGRG 2 cut(s) 461, 760
BmgT120I GGNCC 7 cut(s) 113, 151, 329, 465, 572, 705, 765
BmiI GGNNCC 7 cut(s) 145, 201, 301, 466, 529, 648, 706
BmrFI CCNGG 4 cut(s) 149, 314, 525, 703
BmrI ACTGGG 1 cut(s) 305
BmuI ACTGGG 1 cut(s) 305
BpiI GAAGAC 1 cut(s) 466
BpmI CTGGAG 1 cut(s) 89
Bpu14I TTCGAA 2 cut(s) 78, 608
BpuMI CCSGG 2 cut(s) 525, 703
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 6 cut(s) 147, 163, 387, 523, 568, 750
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 376, 581, 661
Bse1I ACTGG 2 cut(s) 72, 300
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 2 cut(s) 149, 314
BseDI CCNNGG 6 cut(s) 147, 163, 387, 523, 568, 750
BseGI GGATG 1 cut(s) 655
BseLI CCNNNNNNNGG 4 cut(s) 94, 376, 581, 661
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 2 cut(s) 252, 748
BseNI ACTGG 2 cut(s) 72, 300
BseSI GKGCMC 2 cut(s) 106, 620
BseXI GCAGC 4 cut(s) 49, 391, 436, 700
BsgI GTGCAG 1 cut(s) 707
Bsh1236I CGCG 2 cut(s) 402, 638
BshFI GGCC 2 cut(s) 567, 749
BshNI GGYRCC 1 cut(s) 527
BsiHKAI GWGCWC 2 cut(s) 386, 396
BsiHKCI CYCGRG 2 cut(s) 461, 760
BsiSI CCGG 2 cut(s) 525, 702
BslFI GGGAC 2 cut(s) 329, 718
BslI CCNNNNNNNGG 4 cut(s) 94, 376, 581, 661
BsmAI GTCTC 2 cut(s) 222, 621
BsmFI GGGAC 2 cut(s) 329, 718
BsnI GGCC 2 cut(s) 567, 749
Bso31I GGTCTC 1 cut(s) 222
BsoBI CYCGRG 2 cut(s) 461, 760
Bsp119I TTCGAA 2 cut(s) 78, 608
Bsp1286I GDGCHC 6 cut(s) 106, 148, 304, 386, 396, 620
Bsp143I GATC 6 cut(s) 118, 438, 457, 585, 646, 678
BspACI CCGC 3 cut(s) 348, 422, 483
BspANI GGCC 2 cut(s) 567, 749
BspCNI CTCAG 2 cut(s) 253, 747
BspFNI CGCG 2 cut(s) 402, 638
BspLI GGNNCC 7 cut(s) 145, 201, 301, 466, 529, 648, 706
BspPI GGATC 4 cut(s) 593, 641, 654, 686
BspQI GCTCTTC 1 cut(s) 419
BspT104I TTCGAA 2 cut(s) 78, 608
BspT107I GGYRCC 1 cut(s) 527
BspTNI GGTCTC 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 477
BsrI ACTGG 2 cut(s) 72, 300
BssECI CCNNGG 6 cut(s) 147, 163, 387, 523, 568, 750
BssMI GATC 6 cut(s) 118, 438, 457, 585, 646, 678
BssT1I CCWWGG 3 cut(s) 163, 568, 750
Bst2UI CCWGG 2 cut(s) 149, 314
Bst6I CTCTTC 2 cut(s) 419, 705
BstBI TTCGAA 2 cut(s) 78, 608
BstC8I GCNNGC 2 cut(s) 357, 446
BstDEI CTNAG 2 cut(s) 261, 734
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 2 cut(s) 402, 638
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 6 cut(s) 121, 441, 460, 588, 649, 681
BstMAI GTCTC 2 cut(s) 222, 621
BstMBI GATC 6 cut(s) 118, 438, 457, 585, 646, 678
BstMWI GCNNNNNNNGC 3 cut(s) 356, 361, 488
BstNI CCWGG 2 cut(s) 149, 314
BstNSI RCATGY 1 cut(s) 721
BstSCI CCNGG 4 cut(s) 147, 312, 523, 701
BstSLI GKGCMC 2 cut(s) 106, 620
BstUI CGCG 2 cut(s) 402, 638
BstV1I GCAGC 4 cut(s) 49, 391, 436, 700
BstV2I GAAGAC 1 cut(s) 466
BstX2I RGATCY 1 cut(s) 646
BstXI CCANNNNNNTGG 1 cut(s) 67
BstYI RGATCY 1 cut(s) 646
BsuRI GGCC 2 cut(s) 567, 749
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 1 cut(s) 655
Cac8I GCNNGC 2 cut(s) 357, 446
CfoI GCGC 1 cut(s) 404
Cfr13I GGNCC 7 cut(s) 113, 151, 329, 465, 572, 705, 765
Csp6I GTAC 4 cut(s) 73, 289, 715, 738
CviAII CATG 4 cut(s) 361, 505, 718, 727
CviQI GTAC 4 cut(s) 73, 289, 715, 738
DdeI CTNAG 2 cut(s) 261, 734
DpnI GATC 6 cut(s) 120, 440, 459, 587, 648, 680
DpnII GATC 6 cut(s) 118, 438, 457, 585, 646, 678
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 565
Eam1104I CTCTTC 2 cut(s) 419, 705
EarI CTCTTC 2 cut(s) 419, 705
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 3 cut(s) 163, 568, 750
Eco24I GRGCYC 3 cut(s) 148, 304, 396
Eco31I GGTCTC 1 cut(s) 222
Eco47I GGWCC 7 cut(s) 113, 151, 329, 465, 572, 705, 765
Eco53kI GAGCTC 1 cut(s) 394
Eco88I CYCGRG 2 cut(s) 461, 760
EcoICRI GAGCTC 1 cut(s) 394
EcoO109I RGGNCCY 2 cut(s) 572, 765
EcoRII CCWGG 2 cut(s) 147, 312
EcoT14I CCWWGG 3 cut(s) 163, 568, 750
EcoT22I ATGCAT 1 cut(s) 366
EcoT38I GRGCYC 3 cut(s) 148, 304, 396
ErhI CCWWGG 3 cut(s) 163, 568, 750
FaeI CATG 4 cut(s) 364, 508, 721, 730
FaqI GGGAC 2 cut(s) 329, 718
FatI CATG 4 cut(s) 360, 504, 717, 726
FauI CCCGC 2 cut(s) 355, 429
FbaI TGATCA 2 cut(s) 118, 438
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 5 cut(s) 38, 405, 425, 483, 689
FokI GGATG 1 cut(s) 642
FriOI GRGCYC 3 cut(s) 148, 304, 396
Fsp4HI GCNGC 5 cut(s) 38, 405, 425, 483, 689
FspBI CTAG 4 cut(s) 213, 335, 428, 769
GlaI GCGC 1 cut(s) 403
GluI GCNGC 5 cut(s) 38, 405, 425, 483, 689
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 2 cut(s) 567, 749
HapII CCGG 2 cut(s) 525, 702
HhaI GCGC 1 cut(s) 404
Hin1II CATG 4 cut(s) 364, 508, 721, 730
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HinfI GANTC 2 cut(s) 54, 124
HpaII CCGG 2 cut(s) 525, 702
HphI GGTGA 2 cut(s) 49, 109
Hpy166II GTNNAC 3 cut(s) 139, 184, 250
Hpy188I TCNGA 6 cut(s) 53, 113, 123, 262, 590, 664
Hpy188III TCNNGA 1 cut(s) 697
Hpy8I GTNNAC 3 cut(s) 139, 184, 250
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 2 cut(s) 661, 669
HpyCH4IV ACGT 2 cut(s) 243, 471
HpyCH4V TGCA 5 cut(s) 364, 502, 597, 688, 721
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 361, 488
HpyF3I CTNAG 2 cut(s) 261, 734
HpySE526I ACGT 2 cut(s) 243, 471
Hsp92II CATG 4 cut(s) 364, 508, 721, 730
HspAI GCGC 1 cut(s) 402
Ksp22I TGATCA 2 cut(s) 118, 438
Kzo9I GATC 6 cut(s) 118, 438, 457, 585, 646, 678
LguI GCTCTTC 1 cut(s) 419
LmnI GCTCC 5 cut(s) 143, 299, 389, 391, 399
Lsp1109I GCAGC 4 cut(s) 49, 391, 436, 700
MaeI CTAG 4 cut(s) 213, 335, 428, 769
MaeII ACGT 2 cut(s) 243, 471
MaeIII GTNAC 3 cut(s) 316, 518, 541
MalI GATC 6 cut(s) 120, 440, 459, 587, 648, 680
MboI GATC 6 cut(s) 118, 438, 457, 585, 646, 678
MboII GAAGA 3 cut(s) 406, 466, 722
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 6 cut(s) 106, 148, 304, 386, 396, 620
MlsI TGGCCA 1 cut(s) 567
MluI ACGCGT 1 cut(s) 636
MluNI TGGCCA 1 cut(s) 567
MlyI GAGTC 1 cut(s) 118
MmeI TCCRAC 2 cut(s) 421, 492
MnlI CCTC 5 cut(s) 263, 287, 418, 743, 756
Mox20I TGGCCA 1 cut(s) 567
Mph1103I ATGCAT 1 cut(s) 366
MscI TGGCCA 1 cut(s) 567
MseI TTAA 1 cut(s) 42
Msp20I TGGCCA 1 cut(s) 567
MspI CCGG 2 cut(s) 525, 702
MspR9I CCNGG 4 cut(s) 149, 314, 525, 703
MvaI CCWGG 2 cut(s) 149, 314
MvnI CGCG 2 cut(s) 402, 638
MwoI GCNNNNNNNGC 3 cut(s) 356, 361, 488
NciI CCSGG 2 cut(s) 525, 703
NdeII GATC 6 cut(s) 118, 438, 457, 585, 646, 678
NlaIII CATG 4 cut(s) 364, 508, 721, 730
NlaIV GGNNCC 7 cut(s) 145, 201, 301, 466, 529, 648, 706
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 316
NsiI ATGCAT 1 cut(s) 366
NspI RCATGY 1 cut(s) 721
NspV TTCGAA 2 cut(s) 78, 608
PaeR7I CTCGAG 1 cut(s) 760
PciSI GCTCTTC 1 cut(s) 419
PcsI WCGNNNNNNNCGW 1 cut(s) 468
PfeI GAWTC 1 cut(s) 54
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 5 cut(s) 39, 406, 426, 484, 690
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
PpuMI RGGWCCY 2 cut(s) 572, 765
PsiI TTATAA 1 cut(s) 158
Psp124BI GAGCTC 1 cut(s) 396
Psp5II RGGWCCY 2 cut(s) 572, 765
Psp6I CCWGG 2 cut(s) 147, 312
PspGI CCWGG 2 cut(s) 147, 312
PspN4I GGNNCC 7 cut(s) 145, 201, 301, 466, 529, 648, 706
PspPI GGNCC 7 cut(s) 113, 151, 329, 465, 572, 705, 765
PspPPI RGGWCCY 2 cut(s) 572, 765
PspXI VCTCGAGB 1 cut(s) 760
PsrI GAACNNNNNNTAC 2 cut(s) 176, 208
PsuI RGATCY 1 cut(s) 646
PsyI GACNNNGTC 1 cut(s) 242
RsaI GTAC 4 cut(s) 74, 290, 716, 739
RsaNI GTAC 4 cut(s) 73, 289, 715, 738
SacI GAGCTC 1 cut(s) 396
SapI GCTCTTC 1 cut(s) 419
SaqAI TTAA 1 cut(s) 42
SatI GCNGC 5 cut(s) 38, 405, 425, 483, 689
Sau3AI GATC 6 cut(s) 118, 438, 457, 585, 646, 678
Sau96I GGNCC 7 cut(s) 113, 151, 329, 465, 572, 705, 765
ScaI AGTACT 2 cut(s) 74, 739
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 4 cut(s) 149, 314, 525, 703
SduI GDGCHC 6 cut(s) 106, 148, 304, 386, 396, 620
Sfr274I CTCGAG 1 cut(s) 760
SfuI TTCGAA 2 cut(s) 78, 608
SinI GGWCC 7 cut(s) 113, 151, 329, 465, 572, 705, 765
SlaI CTCGAG 1 cut(s) 760
SmlI CTYRAG 1 cut(s) 760
SmoI CTYRAG 1 cut(s) 760
SsiI CCGC 3 cut(s) 348, 422, 483
SspMI CTAG 4 cut(s) 213, 335, 428, 769
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 4 cut(s) 147, 312, 523, 701
StyI CCWWGG 3 cut(s) 163, 568, 750
TaiI ACGT 2 cut(s) 246, 474
TaqI TCGA 3 cut(s) 78, 608, 761
TaqII GACCGA 1 cut(s) 722
TatI WGTACW 3 cut(s) 72, 714, 737
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 1 cut(s) 54
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 4 cut(s) 37, 404, 424, 688
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 2 cut(s) 466, 743
TspGWI ACGGA 3 cut(s) 156, 376, 639
Tth111I GACNNNGTC 1 cut(s) 242
VpaK11BI GGWCC 7 cut(s) 113, 151, 329, 465, 572, 705, 765
XceI RCATGY 1 cut(s) 721
XhoI CTCGAG 1 cut(s) 760
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 4 cut(s) 213, 335, 428, 769
ZrmI AGTACT 2 cut(s) 74, 739
Zsp2I ATGCAT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.