pycom04g20800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010703
Physical Location & Seq
Forward (+)
22235461 .. 22238171
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20800.4

Sequence Viewer

Length: 1089 bp
ATGCATACCATTTTTGTTTCTTGGGTTGTTCTAGGTCTGCTTGCTGCTTGTGCTGGCCGTCAAGCAGAGGCACGGGCTTTTTTCGTGTTTGGAGATTCGTTGGTGGACAACGGAAACAACGACTACTTGGCCACCACTGCCAGGGCCGACTCCTACCCTTACGGCATAGATTATCCTACTCACCGACCCACCGGTCGTTTCTCGAATGGCCTTAACATACCAGATCTTATCAGTGAGCAAATTGGCTCAGAACCCACGTTGCCCTACTTGAGCCCACAACTCACTGGACAAAACCTACTTGTTGGTGCCAACTTTGCTTCTGCCGGGATTGGAATTCTCAACGACACTGGAATCCAATTTCTCAACATAATCCGAATCTACAAACAGTTGGAATACTTTCAGCAATACCAGACCAGAGTGAGTGCTTTAGTTGGACCTGAGCAGGCTCAACGACTTGTCAACGAAGCACTTATTCTGATTACCCTCGGAGGCAATGATTTTGTCAACAACTACTACTTGTTGCCCTTCTCTGCTAGATCTCGCCAATTCTCTCTCCCGGACTATGTTGTCTACCTTATCTCCGAGTACCGCAAAGTTCTAGAGAGGCTGTATGAATTAGGAGCTCGTAAGGTTTTGGTGACAGGCACAGGGCCACTAGGGTGTGTTCCTGCAGAGTTGGCTCTGCACAGCAGAAACGGCGAATGCGCAGTGGAGCTGCAGCGAGCGGCTTCCTTGTTCAACCCGCAACTAGTCGATATGATCAACAGCCTGAATAGCCAATTTGGCTCAGATGCATTTGTTGCTGCAAATGCATATGAAATGAACATGGATTTTGTCTCCGATCCTCAAGCTCATGGATTTGTTACTTCAAAGATAGCTTGCTGTGGCCAAGGACCCTACAATGGGATTGGACTCTGCACAATTGTCTCCAACTTGTGCCCAAACAGAGATTTGTACGCGTTTTGGGATCCTTTCCATCCTTCTGAGAAGGCAAACAGAATTATTGTGCAGAAAATGCTTACCGGGTCCAACAAGTACATGAATCCAATGAACCTCAGCACTATTTTGGCCTTGGACACTAAGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0042335 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

363

Amino Acids

39.87

Weight (kDa)

5.57

Isoelectric Point (pI)

29.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04290 AT5G33370 AT5G33370
fragaria_vesca FvH4_6g01390 FvH4_6g01390
malus_domestica MD04G1234300.v1.1 MD12G1252200.v1.1
prunus_persica Prupe.6G354100_v2.0.a1
pyrus_communis pycom04g20800
rosa_chinensis RchiOBHm_Chr3g0448981 RchiOBHm_Chr3g0449021
rosa_laevigata RLG00000025862
rosa_multiflora Rmu_sc0003116.1_g000011
rosa_roxburghii Rroxscaffold_6G00427140
rosa_rugosa Rorug02G0617400
rosa_samantha Rh3AG015900 Rh3BG016000 Rh3CG015000 Rh3DG016300
rosa_wichuraiana Rw3G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 192, 566
Acc16I TGCGCA 1 cut(s) 706
AccB1I GGYRCC 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 725
AccI GTMKAC 1 cut(s) 570
AccII CGCG 1 cut(s) 959
AciI CCGC 3 cut(s) 589, 725, 743
AclWI GGATC 3 cut(s) 836, 962, 975
AcoI YGGCCR 3 cut(s) 55, 129, 886
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 3 cut(s) 587, 956, 1037
AfiI CCNNNNNNNGG 3 cut(s) 141, 902, 903
AflIII ACRYGT 1 cut(s) 957
AgeI ACCGGT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 739, 870
AhlI ACTAGT 1 cut(s) 748
AjnI CCWGG 1 cut(s) 140
AleI CACNNNNGTG 1 cut(s) 658
AluBI AGCT 4 cut(s) 623, 715, 851, 878
AluI AGCT 4 cut(s) 623, 715, 851, 878
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 2 cut(s) 841, 931
AlwI GGATC 3 cut(s) 836, 962, 975
AoxI GGCC 7 cut(s) 55, 129, 144, 208, 650, 886, 1068
ApeKI GCWGC 4 cut(s) 44, 715, 718, 803
ApoI RAATTY 1 cut(s) 333
AsiGI ACCGGT 1 cut(s) 191
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 1 cut(s) 707
AspS9I GGNCC 5 cut(s) 144, 434, 650, 893, 1026
AsuC2I CCSGG 3 cut(s) 325, 557, 1024
AsuHPI GGTGA 2 cut(s) 173, 649
AvaII GGWCC 3 cut(s) 434, 893, 1026
BaeGI GKGCMC 1 cut(s) 941
BalI TGGCCA 2 cut(s) 131, 888
BamHI GGATCC 1 cut(s) 967
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 2 cut(s) 275, 625
Bbv12I GWGCWC 1 cut(s) 625
BbvCI CCTCAGC 1 cut(s) 1055
BbvI GCAGC 4 cut(s) 31, 702, 730, 790
BccI CCATC 1 cut(s) 984
BceAI ACGGC 3 cut(s) 42, 178, 712
BciT130I CCWGG 1 cut(s) 142
BclI TGATCA 1 cut(s) 759
BcnI CCSGG 3 cut(s) 325, 557, 1024
BcoDI GTCTC 2 cut(s) 841, 931
BcuI ACTAGT 1 cut(s) 748
BfaI CTAG 6 cut(s) 32, 534, 599, 656, 749, 1087
BfmI CTRYAG 2 cut(s) 669, 716
BglI GCCNNNNNGGC 1 cut(s) 783
BglII AGATCT 2 cut(s) 223, 536
BisI GCNGC 5 cut(s) 45, 716, 719, 726, 804
BlsI GCNGC 5 cut(s) 46, 717, 720, 727, 805
Bme1390I CCNGG 4 cut(s) 142, 325, 557, 1024
Bme18I GGWCC 3 cut(s) 434, 893, 1026
BmgT120I GGNCC 5 cut(s) 144, 434, 650, 893, 1026
BmiI GGNNCC 4 cut(s) 307, 895, 969, 1027
BmrFI CCNGG 4 cut(s) 142, 325, 557, 1024
BmsI GCATC 1 cut(s) 781
Bpu10I CCTNAGC 2 cut(s) 438, 1055
BpuEI CTTGAG 2 cut(s) 289, 831
BpuMI CCSGG 3 cut(s) 325, 557, 1024
BsaJI CCNNGG 4 cut(s) 141, 484, 889, 1071
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 902, 903
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 2 cut(s) 289, 352
Bse3DI GCAATG 1 cut(s) 499
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 4 cut(s) 141, 484, 889, 1071
BseGI GGATG 1 cut(s) 976
BseLI CCNNNNNNNGG 3 cut(s) 141, 902, 903
BseMI GCAATG 1 cut(s) 499
BseMII CTCAG 5 cut(s) 261, 429, 801, 975, 1069
BseNI ACTGG 2 cut(s) 289, 352
BseSI GKGCMC 1 cut(s) 941
BseXI GCAGC 4 cut(s) 31, 702, 730, 790
BsgI GTGCAG 3 cut(s) 668, 901, 1028
Bsh1236I CGCG 1 cut(s) 959
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 7 cut(s) 57, 131, 146, 210, 652, 888, 1070
BshNI GGYRCC 1 cut(s) 305
BshTI ACCGGT 1 cut(s) 191
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 625
BsiSI CCGG 4 cut(s) 192, 324, 557, 1023
BslI CCNNNNNNNGG 3 cut(s) 141, 902, 903
BsmAI GTCTC 2 cut(s) 841, 931
BsmI GAATGC 1 cut(s) 707
BsnI GGCC 7 cut(s) 57, 131, 146, 210, 652, 888, 1070
Bsp1286I GDGCHC 3 cut(s) 275, 625, 941
Bsp143I GATC 5 cut(s) 223, 536, 759, 841, 967
BspACI CCGC 3 cut(s) 589, 725, 743
BspANI GGCC 7 cut(s) 57, 131, 146, 210, 652, 888, 1070
BspCNI CTCAG 5 cut(s) 260, 430, 800, 976, 1068
BspFNI CGCG 1 cut(s) 959
BspLI GGNNCC 4 cut(s) 307, 895, 969, 1027
BspMAI CTGCAG 2 cut(s) 673, 720
BspPI GGATC 3 cut(s) 836, 962, 975
BspT107I GGYRCC 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 725
BsrDI GCAATG 1 cut(s) 499
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 2 cut(s) 289, 352
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 4 cut(s) 141, 484, 889, 1071
BssMI GATC 5 cut(s) 223, 536, 759, 841, 967
BssT1I CCWWGG 2 cut(s) 889, 1071
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 387
BstAPI GCANNNNNTGC 2 cut(s) 800, 1015
BstC8I GCNNGC 5 cut(s) 42, 55, 444, 723, 880
BstDEI CTNAG 6 cut(s) 247, 438, 787, 984, 1055, 1080
BstF5I GGATG 1 cut(s) 976
BstFNI CGCG 1 cut(s) 959
BstHHI GCGC 1 cut(s) 707
BstKTI GATC 5 cut(s) 226, 539, 762, 844, 970
BstMAI GTCTC 2 cut(s) 841, 931
BstMBI GATC 5 cut(s) 223, 536, 759, 841, 967
BstMCI CGRYCG 1 cut(s) 196
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 4 cut(s) 140, 323, 555, 1022
BstSFI CTRYAG 2 cut(s) 669, 716
BstSLI GKGCMC 1 cut(s) 941
BstUI CGCG 1 cut(s) 959
BstV1I GCAGC 4 cut(s) 31, 702, 730, 790
BstX2I RGATCY 3 cut(s) 223, 536, 967
BstYI RGATCY 3 cut(s) 223, 536, 967
BsuRI GGCC 7 cut(s) 57, 131, 146, 210, 652, 888, 1070
BtsCI GGATG 1 cut(s) 976
BtsI GCAGTG 2 cut(s) 135, 714
BtsIMutI CAGTG 5 cut(s) 135, 238, 282, 345, 714
Cac8I GCNNGC 5 cut(s) 42, 55, 444, 723, 880
CfoI GCGC 1 cut(s) 707
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 5 cut(s) 144, 434, 650, 893, 1026
Csp6I GTAC 3 cut(s) 586, 955, 1036
CspAI ACCGGT 1 cut(s) 191
CviAII CATG 3 cut(s) 826, 854, 1039
CviQI GTAC 3 cut(s) 586, 955, 1036
DdeI CTNAG 6 cut(s) 247, 438, 787, 984, 1055, 1080
DpnI GATC 5 cut(s) 225, 538, 761, 843, 969
DpnII GATC 5 cut(s) 223, 536, 759, 841, 967
DrdI GACNNNNNNGTC 2 cut(s) 192, 566
DseDI GACNNNNNNGTC 2 cut(s) 192, 566
EaeI YGGCCR 3 cut(s) 55, 129, 886
Ecl136II GAGCTC 1 cut(s) 623
Eco130I CCWWGG 2 cut(s) 889, 1071
Eco24I GRGCYC 2 cut(s) 275, 625
Eco47I GGWCC 3 cut(s) 434, 893, 1026
Eco53kI GAGCTC 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 893
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 2 cut(s) 889, 1071
EcoT22I ATGCAT 3 cut(s) 6, 796, 814
EcoT38I GRGCYC 2 cut(s) 275, 625
ErhI CCWWGG 2 cut(s) 889, 1071
FaeI CATG 3 cut(s) 829, 857, 1042
FatI CATG 3 cut(s) 825, 853, 1038
FauI CCCGC 1 cut(s) 750
FauNDI CATATG 1 cut(s) 814
FbaI TGATCA 1 cut(s) 759
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 5 cut(s) 45, 716, 719, 726, 804
FokI GGATG 1 cut(s) 963
FriOI GRGCYC 2 cut(s) 275, 625
Fsp4HI GCNGC 5 cut(s) 45, 716, 719, 726, 804
FspBI CTAG 6 cut(s) 32, 534, 599, 656, 749, 1087
FspI TGCGCA 1 cut(s) 706
GlaI GCGC 1 cut(s) 706
GluI GCNGC 5 cut(s) 45, 716, 719, 726, 804
HaeIII GGCC 7 cut(s) 57, 131, 146, 210, 652, 888, 1070
HapII CCGG 4 cut(s) 192, 324, 557, 1023
HhaI GCGC 1 cut(s) 707
Hin1II CATG 3 cut(s) 829, 857, 1042
Hin6I GCGC 1 cut(s) 705
HinP1I GCGC 1 cut(s) 705
HincII GTYRAC 2 cut(s) 460, 505
HindII GTYRAC 2 cut(s) 460, 505
HinfI GANTC 6 cut(s) 95, 149, 351, 375, 912, 1042
HpaII CCGG 4 cut(s) 192, 324, 557, 1023
HphI GGTGA 2 cut(s) 173, 649
Hpy166II GTNNAC 4 cut(s) 106, 460, 505, 571
Hpy188I TCNGA 8 cut(s) 250, 374, 477, 488, 583, 790, 841, 985
Hpy188III TCNNGA 2 cut(s) 202, 599
Hpy8I GTNNAC 4 cut(s) 106, 460, 505, 571
HpyAV CCTTC 3 cut(s) 535, 982, 990
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 9 cut(s) 4, 671, 685, 718, 794, 806, 812, 918, 1009
HpyF3I CTNAG 6 cut(s) 247, 438, 787, 984, 1055, 1080
HpySE526I ACGT 1 cut(s) 257
Hsp92II CATG 3 cut(s) 829, 857, 1042
HspAI GCGC 1 cut(s) 705
Ksp22I TGATCA 1 cut(s) 759
Kzo9I GATC 5 cut(s) 223, 536, 759, 841, 967
LmnI GCTCC 2 cut(s) 620, 712
Lsp1109I GCAGC 4 cut(s) 31, 702, 730, 790
LweI GCATC 1 cut(s) 781
MaeI CTAG 6 cut(s) 32, 534, 599, 656, 749, 1087
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 2 cut(s) 637, 862
MalI GATC 5 cut(s) 225, 538, 761, 843, 969
MbiI CCGCTC 1 cut(s) 725
MboI GATC 5 cut(s) 223, 536, 759, 841, 967
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 3 cut(s) 223, 536, 967
MhlI GDGCHC 3 cut(s) 275, 625, 941
MlsI TGGCCA 2 cut(s) 131, 888
MluCI AATT 8 cut(s) 240, 333, 356, 545, 614, 779, 921, 999
MluI ACGCGT 1 cut(s) 957
MluNI TGGCCA 2 cut(s) 131, 888
MlyI GAGTC 2 cut(s) 143, 906
MmeI TCCRAC 4 cut(s) 369, 412, 954, 1053
MnlI CCTC 6 cut(s) 61, 482, 494, 597, 855, 1064
Mox20I TGGCCA 2 cut(s) 131, 888
Mph1103I ATGCAT 3 cut(s) 6, 796, 814
MroXI GAANNNNTTC 1 cut(s) 396
MscI TGGCCA 2 cut(s) 131, 888
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 1 cut(s) 658
Msp20I TGGCCA 2 cut(s) 131, 888
MspI CCGG 4 cut(s) 192, 324, 557, 1023
MspR9I CCNGG 4 cut(s) 142, 325, 557, 1024
MunI CAATTG 1 cut(s) 921
Mva1269I GAATGC 1 cut(s) 707
MvaI CCWGG 1 cut(s) 142
MvnI CGCG 1 cut(s) 959
NciI CCSGG 3 cut(s) 325, 557, 1024
NdeI CATATG 1 cut(s) 814
NdeII GATC 5 cut(s) 223, 536, 759, 841, 967
NlaIII CATG 3 cut(s) 829, 857, 1042
NlaIV GGNNCC 4 cut(s) 307, 895, 969, 1027
NmuCI GTSAC 1 cut(s) 637
NsbI TGCGCA 1 cut(s) 706
NsiI ATGCAT 3 cut(s) 6, 796, 814
OliI CACNNNNGTG 1 cut(s) 658
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 1 cut(s) 707
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 4 cut(s) 95, 351, 375, 1042
PfoI TCCNGGA 1 cut(s) 555
PinAI ACCGGT 1 cut(s) 191
PkrI GCNGC 5 cut(s) 46, 717, 720, 727, 805
PleI GAGTC 2 cut(s) 143, 906
PpsI GAGTC 2 cut(s) 143, 906
PpuMI RGGWCCY 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 625
Psp5II RGGWCCY 1 cut(s) 893
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 4 cut(s) 307, 895, 969, 1027
PspPI GGNCC 5 cut(s) 144, 434, 650, 893, 1026
PspPPI RGGWCCY 1 cut(s) 893
PstI CTGCAG 2 cut(s) 673, 720
PsuI RGATCY 3 cut(s) 223, 536, 967
RsaI GTAC 3 cut(s) 587, 956, 1037
RsaNI GTAC 3 cut(s) 586, 955, 1036
RseI CAYNNNNRTG 1 cut(s) 658
SacI GAGCTC 1 cut(s) 625
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 5 cut(s) 45, 716, 719, 726, 804
Sau3AI GATC 5 cut(s) 223, 536, 759, 841, 967
Sau96I GGNCC 5 cut(s) 144, 434, 650, 893, 1026
SchI GAGTC 2 cut(s) 143, 906
ScrFI CCNGG 4 cut(s) 142, 325, 557, 1024
SduI GDGCHC 3 cut(s) 275, 625, 941
SfaNI GCATC 1 cut(s) 781
SfcI CTRYAG 2 cut(s) 669, 716
SinI GGWCC 3 cut(s) 434, 893, 1026
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 2 cut(s) 268, 846
SmoI CTYRAG 2 cut(s) 268, 846
SpeI ACTAGT 1 cut(s) 748
Sse9I AATT 8 cut(s) 240, 333, 356, 545, 614, 779, 921, 999
SsiI CCGC 3 cut(s) 589, 725, 743
SspMI CTAG 6 cut(s) 32, 534, 599, 656, 749, 1087
SstI GAGCTC 1 cut(s) 625
StyD4I CCNGG 4 cut(s) 140, 323, 555, 1022
StyI CCWWGG 2 cut(s) 889, 1071
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 1 cut(s) 260
TaqI TCGA 2 cut(s) 203, 753
TasI AATT 8 cut(s) 240, 333, 356, 545, 614, 779, 921, 999
TatI WGTACW 1 cut(s) 1035
TauI GCSGC 1 cut(s) 728
TfiI GAWTC 4 cut(s) 95, 351, 375, 1042
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 5 cut(s) 142, 238, 289, 352, 714
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 4 cut(s) 44, 715, 718, 803
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 5 cut(s) 627, 831, 836, 1055, 1064
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 5 cut(s) 142, 238, 289, 352, 714
VpaK11BI GGWCC 3 cut(s) 434, 893, 1026
XapI RAATTY 1 cut(s) 333
XbaI TCTAGA 1 cut(s) 598
XmiI GTMKAC 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 6 cut(s) 32, 534, 599, 656, 749, 1087
Zsp2I ATGCAT 3 cut(s) 6, 796, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.