AT5G33370

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010703
Physical Location & Seq
Reverse (-)
12602468 .. 12604832
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G33370.1

Sequence Viewer

Length: 1101 bp
ATGACGAACTCGGTGGCTAAGTTGGCACTACTAGGGTTTTGCATTCTGCAAGTGACGAGCTTACTTGTTCCGCAAGCAAATGCTAGGGCTTTCCTTGTGTTTGGAGATTCTCTCGTTGACAATGGTAACAATGACTTTCTTGCTACCACTGCTCGTGCCGATAATTACCCTTATGGTATCGATTTCCCAACTCATCGTCCTACGGGCCGTTTCTCCAATGGTCTAAACATTCCAGATCTCATCAGTGAACATTTGGGTCAAGAGTCTCCAATGCCGTACCTTAGTCCAATGCTGAAGAAGGACAAACTCTTAAGGGGAGCTAACTTCGCCTCCGCGGGTATTGGAATCCTTAACGACACCGGAATCCAGTTTCTGAATATTATTAGGATCACAAAGCAGCTAGAGTACTTCGAGCAGTACAAGGTTCGAGTAAGCGGGTTGGTCGGAGAAGAAGAGATGAATCGGCTCGTGAACGGAGCTCTTGTCCTAATAACACTTGGAGGCAATGATTTCGTCAACAACTACTACTTGGTCCCTTTCTCTGCAAGATCTCGTCAATTCTCTCTTCCTGACTACGTTGTCTTTGTCATTTCTGAATACCGCAAAGTCTTACGGAAAATGTACGATTTGGGTGCTCGACGTGTCCTTGTGACTGGAACAGGACCAATGGGTTGCGTCCCGGCCGAGCTGGCGCAACGTAGCCGCAACGGCGAGTGTGCTACCGAACTCCAACGAGCCGCGTCACTATTCAACCCACAACTAATTCAAATGATAACAGACCTCAACAACGAAGTTGGATCTTCGGCCTTCATTGCCGCTAATACTCAACAAATGCACATGGACTTCATTAGCGACCCACAAGCATATGGATTCGTCACGTCGAAGGTGGCTTGTTGTGGACAAGGGCCGTACAATGGGATAGGGCTATGCACTCCATTATCAAATCTTTGCCCAAACAGAGATCTCTTTGCCTTTTGGGATCCTTTTCACCCATCAGAAAAAGCAAGTAGAATCATAGCTCAACAAATCCTCAATGGCTCTCCTGAATACATGCATCCCATGAATCTTAGCACCATCCTCACCGTTGATTCCATGACCTAA

Protein Analysis

366

Amino Acids

40.36

Weight (kDa)

6.22

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 343 6.4e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04290 AT5G33370 AT5G33370
fragaria_vesca FvH4_6g01390 FvH4_6g01390
malus_domestica MD04G1234300.v1.1 MD12G1252200.v1.1
prunus_persica Prupe.6G354100_v2.0.a1
pyrus_communis pycom04g20800
rosa_chinensis RchiOBHm_Chr3g0448981 RchiOBHm_Chr3g0449021
rosa_laevigata RLG00000025862
rosa_multiflora Rmu_sc0003116.1_g000011
rosa_roxburghii Rroxscaffold_6G00427140
rosa_rugosa Rorug02G0617400
rosa_samantha Rh3AG015900 Rh3BG016000 Rh3CG015000 Rh3DG016300
rosa_wichuraiana Rw3G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 578
AccII CGCG 2 cut(s) 335, 740
AciI CCGC 8 cut(s) 71, 333, 335, 435, 601, 703, 738, 816
AclWI GGATC 4 cut(s) 395, 805, 974, 987
AcoI YGGCCR 1 cut(s) 681
AcuI CTGAAG 1 cut(s) 314
AfaI GTAC 5 cut(s) 278, 407, 419, 623, 911
AfiI CCNNNNNNNGG 1 cut(s) 914
AflII CTTAAG 1 cut(s) 310
AflIII ACRYGT 1 cut(s) 640
AgsI TTSAA 2 cut(s) 751, 767
AjiI CACGTC 2 cut(s) 641, 879
AluBI AGCT 6 cut(s) 60, 320, 400, 479, 688, 1019
AluI AGCT 6 cut(s) 60, 320, 400, 479, 688, 1019
Alw21I GWGCWC 2 cut(s) 481, 637
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 4 cut(s) 395, 805, 974, 987
AlwNI CAGNNNCTG 1 cut(s) 373
AoxI GGCC 4 cut(s) 205, 681, 804, 905
ApeKI GCWGC 1 cut(s) 397
AspLEI GCGC 1 cut(s) 694
AspS9I GGNCC 4 cut(s) 205, 532, 662, 905
AsuC2I CCSGG 1 cut(s) 680
AsuHPI GGTGA 2 cut(s) 980, 1072
AvaII GGWCC 2 cut(s) 532, 662
BamHI GGATCC 1 cut(s) 979
BanII GRGCYC 1 cut(s) 481
BauI CACGAG 2 cut(s) 153, 467
Bbv12I GWGCWC 2 cut(s) 481, 637
BbvI GCAGC 1 cut(s) 409
BccI CCATC 2 cut(s) 1000, 1082
BceAI ACGGC 4 cut(s) 192, 259, 724, 892
BcgI CGANNNNNNTGC 4 cut(s) 493, 527, 614, 648
BcnI CCSGG 1 cut(s) 680
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 3 cut(s) 32, 84, 401
BfrI CTTAAG 1 cut(s) 310
BglI GCCNNNNNGGC 2 cut(s) 689, 708
BglII AGATCT 3 cut(s) 235, 548, 961
BisI GCNGC 4 cut(s) 398, 703, 738, 816
BlsI GCNGC 4 cut(s) 399, 704, 739, 817
BmcAI AGTACT 1 cut(s) 407
Bme1390I CCNGG 1 cut(s) 680
Bme18I GGWCC 2 cut(s) 532, 662
BmgBI CACGTC 2 cut(s) 641, 879
BmgT120I GGNCC 4 cut(s) 205, 532, 662, 905
BmiI GGNNCC 2 cut(s) 534, 981
BmrFI CCNGG 1 cut(s) 680
BmsI GCATC 1 cut(s) 1063
BplI GAGNNNNNCTC 2 cut(s) 96, 128
BpuMI CCSGG 1 cut(s) 680
Bsa29I ATCGAT 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 4 cut(s) 314, 344, 468, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 914
Bse1I ACTGG 2 cut(s) 367, 658
Bse3DI GCAATG 2 cut(s) 511, 810
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 333
BseGI GGATG 2 cut(s) 1054, 1074
BseLI CCNNNNNNNGG 1 cut(s) 914
BseMI GCAATG 2 cut(s) 511, 810
BseNI ACTGG 2 cut(s) 367, 658
BseX3I CGGCCG 1 cut(s) 681
BseXI GCAGC 1 cut(s) 409
Bsh1236I CGCG 2 cut(s) 335, 740
Bsh1285I CGRYCG 1 cut(s) 684
BshFI GGCC 4 cut(s) 207, 683, 806, 907
BshVI ATCGAT 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 684
BsiHKAI GWGCWC 2 cut(s) 481, 637
BsiSI CCGG 2 cut(s) 360, 680
BslFI GGGAC 2 cut(s) 518, 662
BslI CCNNNNNNNGG 1 cut(s) 914
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 2 cut(s) 518, 662
BsmI GAATGC 1 cut(s) 42
BsnI GGCC 4 cut(s) 207, 683, 806, 907
Bsp1286I GDGCHC 2 cut(s) 481, 637
Bsp143I GATC 6 cut(s) 235, 387, 548, 797, 961, 979
BspACI CCGC 8 cut(s) 71, 333, 335, 435, 601, 703, 738, 816
BspANI GGCC 4 cut(s) 207, 683, 806, 907
BspDI ATCGAT 1 cut(s) 180
BspFNI CGCG 2 cut(s) 335, 740
BspLI GGNNCC 2 cut(s) 534, 981
BspPI GGATC 4 cut(s) 395, 805, 974, 987
BspTI CTTAAG 1 cut(s) 310
BsrDI GCAATG 2 cut(s) 511, 810
BsrI ACTGG 2 cut(s) 367, 658
BssECI CCNNGG 1 cut(s) 333
BssMI GATC 6 cut(s) 235, 387, 548, 797, 961, 979
BssSI CACGAG 2 cut(s) 153, 467
Bst2BI CACGAG 2 cut(s) 153, 467
Bst4CI ACNGT 1 cut(s) 1084
Bst6I CTCTTC 2 cut(s) 447, 570
BstAFI CTTAAG 1 cut(s) 310
BstC8I GCNNGC 2 cut(s) 75, 690
BstDEI CTNAG 3 cut(s) 18, 281, 1067
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 2 cut(s) 1054, 1074
BstFNI CGCG 2 cut(s) 335, 740
BstHHI GCGC 1 cut(s) 694
BstKTI GATC 6 cut(s) 238, 390, 551, 800, 964, 982
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 6 cut(s) 235, 387, 548, 797, 961, 979
BstMCI CGRYCG 1 cut(s) 684
BstMWI GCNNNNNNNGC 6 cut(s) 23, 149, 326, 689, 708, 812
BstNSI RCATGY 1 cut(s) 1054
BstSCI CCNGG 1 cut(s) 678
BstUI CGCG 2 cut(s) 335, 740
BstV1I GCAGC 1 cut(s) 409
BstX2I RGATCY 5 cut(s) 235, 548, 797, 961, 979
BstYI RGATCY 5 cut(s) 235, 548, 797, 961, 979
BstZI CGGCCG 1 cut(s) 681
Bsu15I ATCGAT 1 cut(s) 180
BsuRI GGCC 4 cut(s) 207, 683, 806, 907
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 333
BtrI CACGTC 2 cut(s) 641, 879
BtsCI GGATG 2 cut(s) 1054, 1074
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 250
Cac8I GCNNGC 2 cut(s) 75, 690
CaiI CAGNNNCTG 1 cut(s) 373
CfoI GCGC 1 cut(s) 694
Cfr13I GGNCC 4 cut(s) 205, 532, 662, 905
Cfr42I CCGCGG 1 cut(s) 336
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 2 cut(s) 664, 729
Csp6I GTAC 5 cut(s) 277, 406, 418, 622, 910
CviAII CATG 4 cut(s) 838, 1051, 1060, 1093
CviQI GTAC 5 cut(s) 277, 406, 418, 622, 910
DdeI CTNAG 3 cut(s) 18, 281, 1067
DpnI GATC 6 cut(s) 237, 389, 550, 799, 963, 981
DpnII GATC 6 cut(s) 235, 387, 548, 797, 961, 979
DrdI GACNNNNNNGTC 1 cut(s) 578
DseDI GACNNNNNNGTC 1 cut(s) 578
EaeI YGGCCR 1 cut(s) 681
EagI CGGCCG 1 cut(s) 681
Eam1104I CTCTTC 2 cut(s) 447, 570
EarI CTCTTC 2 cut(s) 447, 570
Ecl136II GAGCTC 1 cut(s) 479
EclXI CGGCCG 1 cut(s) 681
Eco24I GRGCYC 1 cut(s) 481
Eco47I GGWCC 2 cut(s) 532, 662
Eco52I CGGCCG 1 cut(s) 681
Eco53kI GAGCTC 1 cut(s) 479
Eco57I CTGAAG 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 479
EcoT22I ATGCAT 1 cut(s) 1056
EcoT38I GRGCYC 1 cut(s) 481
FaeI CATG 4 cut(s) 841, 1054, 1063, 1096
FaiI YATR 9 cut(s) 174, 839, 865, 867, 928, 1016, 1052, 1061, 1094
FaqI GGGAC 2 cut(s) 518, 662
FatI CATG 4 cut(s) 837, 1050, 1059, 1092
FauI CCCGC 2 cut(s) 328, 428
FauNDI CATATG 1 cut(s) 865
Fnu4HI GCNGC 4 cut(s) 398, 703, 738, 816
FokI GGATG 2 cut(s) 1041, 1061
FriOI GRGCYC 1 cut(s) 481
Fsp4HI GCNGC 4 cut(s) 398, 703, 738, 816
FspBI CTAG 3 cut(s) 32, 84, 401
GlaI GCGC 1 cut(s) 693
GluI GCNGC 4 cut(s) 398, 703, 738, 816
HaeIII GGCC 4 cut(s) 207, 683, 806, 907
HapII CCGG 2 cut(s) 360, 680
HgaI GACGC 2 cut(s) 664, 729
HhaI GCGC 1 cut(s) 694
Hin1II CATG 4 cut(s) 841, 1054, 1063, 1096
Hin6I GCGC 1 cut(s) 692
HinP1I GCGC 1 cut(s) 692
HincII GTYRAC 2 cut(s) 118, 517
HindII GTYRAC 2 cut(s) 118, 517
HinfI GANTC 9 cut(s) 107, 263, 345, 363, 460, 870, 1011, 1063, 1088
HpaII CCGG 2 cut(s) 360, 680
HphI GGTGA 2 cut(s) 980, 1072
Hpy166II GTNNAC 5 cut(s) 118, 248, 472, 517, 899
Hpy188I TCNGA 4 cut(s) 375, 446, 595, 997
Hpy188III TCNNGA 5 cut(s) 233, 260, 469, 569, 1043
Hpy8I GTNNAC 5 cut(s) 118, 248, 472, 517, 899
Hpy99I CGWCG 2 cut(s) 642, 883
HpyAV CCTTC 3 cut(s) 292, 817, 877
HpyCH4III ACNGT 1 cut(s) 1084
HpyCH4IV ACGT 4 cut(s) 576, 640, 697, 878
HpyCH4V TGCA 6 cut(s) 42, 49, 545, 835, 930, 1054
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 149, 326, 689, 708, 812
HpyF3I CTNAG 3 cut(s) 18, 281, 1067
HpySE526I ACGT 4 cut(s) 576, 640, 697, 878
Hsp92II CATG 4 cut(s) 841, 1054, 1063, 1096
HspAI GCGC 1 cut(s) 692
KspI CCGCGG 1 cut(s) 336
Kzo9I GATC 6 cut(s) 235, 387, 548, 797, 961, 979
LmnI GCTCC 2 cut(s) 317, 476
LpnPI CCDG 9 cut(s) 246, 373, 380, 582, 639, 645, 674, 693, 1056
Lsp1109I GCAGC 1 cut(s) 409
LweI GCATC 1 cut(s) 1063
MaeI CTAG 3 cut(s) 32, 84, 401
MaeII ACGT 4 cut(s) 576, 640, 697, 878
MaeIII GTNAC 5 cut(s) 52, 125, 649, 741, 874
MalI GATC 6 cut(s) 237, 389, 550, 799, 963, 981
MboI GATC 6 cut(s) 235, 387, 548, 797, 961, 979
MboII GAAGA 5 cut(s) 307, 461, 464, 557, 792
MflI RGATCY 5 cut(s) 235, 548, 797, 961, 979
MhlI GDGCHC 2 cut(s) 481, 637
MluCI AATT 3 cut(s) 163, 557, 762
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 3 cut(s) 424, 754, 775
MnlI CCTC 5 cut(s) 340, 494, 791, 1040, 1088
Mph1103I ATGCAT 1 cut(s) 1056
MseI TTAA 2 cut(s) 311, 351
MspA1I CMGCKG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 310
MspI CCGG 2 cut(s) 360, 680
MspR9I CCNGG 1 cut(s) 680
Mva1269I GAATGC 1 cut(s) 42
MvnI CGCG 2 cut(s) 335, 740
MwoI GCNNNNNNNGC 6 cut(s) 23, 149, 326, 689, 708, 812
NciI CCSGG 1 cut(s) 680
NdeI CATATG 1 cut(s) 865
NdeII GATC 6 cut(s) 235, 387, 548, 797, 961, 979
NlaIII CATG 4 cut(s) 841, 1054, 1063, 1096
NlaIV GGNNCC 2 cut(s) 534, 981
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 4 cut(s) 52, 649, 741, 874
NsiI ATGCAT 1 cut(s) 1056
NspI RCATGY 1 cut(s) 1054
PctI GAATGC 1 cut(s) 42
PfeI GAWTC 8 cut(s) 107, 345, 363, 460, 870, 1011, 1063, 1088
PkrI GCNGC 4 cut(s) 399, 704, 739, 817
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
Psp124BI GAGCTC 1 cut(s) 481
PspN4I GGNNCC 2 cut(s) 534, 981
PspPI GGNCC 4 cut(s) 205, 532, 662, 905
PstNI CAGNNNCTG 1 cut(s) 373
PsuI RGATCY 5 cut(s) 235, 548, 797, 961, 979
RsaI GTAC 5 cut(s) 278, 407, 419, 623, 911
RsaNI GTAC 5 cut(s) 277, 406, 418, 622, 910
SacI GAGCTC 1 cut(s) 481
SacII CCGCGG 1 cut(s) 336
SaqAI TTAA 2 cut(s) 311, 351
SatI GCNGC 4 cut(s) 398, 703, 738, 816
Sau3AI GATC 6 cut(s) 235, 387, 548, 797, 961, 979
Sau96I GGNCC 4 cut(s) 205, 532, 662, 905
ScaI AGTACT 1 cut(s) 407
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 1 cut(s) 680
SduI GDGCHC 2 cut(s) 481, 637
SfaNI GCATC 1 cut(s) 1063
Sfr303I CCGCGG 1 cut(s) 336
SgrBI CCGCGG 1 cut(s) 336
SinI GGWCC 2 cut(s) 532, 662
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
Sse9I AATT 3 cut(s) 163, 557, 762
SsiI CCGC 8 cut(s) 71, 333, 335, 435, 601, 703, 738, 816
SspI AATATT 1 cut(s) 379
SspMI CTAG 3 cut(s) 32, 84, 401
SstI GAGCTC 1 cut(s) 481
StyD4I CCNGG 1 cut(s) 678
TaaI ACNGT 1 cut(s) 1084
TaiI ACGT 4 cut(s) 579, 643, 700, 881
TaqI TCGA 5 cut(s) 180, 411, 427, 637, 881
TasI AATT 3 cut(s) 163, 557, 762
TatI WGTACW 2 cut(s) 405, 417
TauI GCSGC 3 cut(s) 705, 740, 818
TfiI GAWTC 8 cut(s) 107, 345, 363, 460, 870, 1011, 1063, 1088
Tru1I TTAA 2 cut(s) 311, 351
Tru9I TTAA 2 cut(s) 311, 351
TscAI CASTG 2 cut(s) 154, 250
TseFI GTSAC 4 cut(s) 52, 649, 741, 874
TseI GCWGC 1 cut(s) 397
Tsp45I GTSAC 4 cut(s) 52, 649, 741, 874
TspDTI ATGAA 4 cut(s) 473, 799, 835, 1076
TspGWI ACGGA 2 cut(s) 489, 628
TspRI CASTG 2 cut(s) 154, 250
Vha464I CTTAAG 1 cut(s) 310
VpaK11BI GGWCC 2 cut(s) 532, 662
XceI RCATGY 1 cut(s) 1054
XspI CTAG 3 cut(s) 32, 84, 401
ZrmI AGTACT 1 cut(s) 407
Zsp2I ATGCAT 1 cut(s) 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.