FvH4_6g01390

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010703
Physical Location & Seq
Reverse (-)
772548 .. 774957
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01390.t2

Sequence Viewer

Length: 1098 bp
ATGCATACAAACGCAATGTCGGCTGCTTGGGTTGCTTTGGTGCTGCTTGTCACTTGTGCTGGTTTTCAAGCCGAGGCACGAGCATTCTTCGTTTTCGGAGATTCGCTGGTGGACAATGGCAACAATGACTACTTGATCACCACAGCCCGGGCTGACTCGTACCCTTATGGGATCGATTACCCGACTCACAGACCAACCGGCCGGTTTTCTAATGGCCTCAACATACCAGACCTCATCAGTGAGTCACTTGGGTCAGAACCTACATTGCCGTACTTGAGTCCTCAGCTTAACGGAGAAAAACTACTTGTTGGTGCCAACTTTGCTTCTGCTGGGATTGGAATCCTCAATGACACTGGAATCCAGTTTCTTAACATAATCAGAATCACTAAGCAACTGGAGTACTTCGAGCAATACCAGACAAGGGTGAGTGCCCTAATCGGACCTGATCAGACTAAAAGCCTCGTCAACGGAGCCCTTGTCCTTATAACACTTGGTGGTAACGACTTTGTGAACAACTACTACTTGGTGCCGTACTCTGCTAGGTCTCGCCAATACTCTCTCCCTGACTACGTCGTCTACCTCATCTCCGAGTATCGCAAAATCCTCAACAGGCTGTACGAACTAGGAGCCCGTCAAGTCCTGGTCACAGGCACAGGATCACTAGGGTGTGTCCCCGCCGAGCTTGCCATGCGTAGCCGAAACGGGGAGTGCTCCGTCGAGCTCCAACGCGCCGCCTCGCTCTTCAACCCGCAGCTAGTCCAAATGATCGGTTCGCTCAACAGCGAAATCGGGGCCAACGTCTTCATTGCCTCAAACTCGAACGAAATGCACATGGATTTCATAAGTGACCCTGGTGCCTACGGGTTTGTAACATCAAAGATAGCCTGTTGTGGCCAAGGACCTTACAACGGGATCGGACTATGCACACCCCTTTCGAACCTGTGCCCCAACCGAGACTTGTATGCGTTCTGGGATCCATTCCATCCTTCGGAGAAGGCTAACAGGCTCATTGTGCAGCAAATCCTCACTGGGAGTGATAAGTACATGCACCCCATGAATCTCAGTACTATGTTGTCCTTGGACAACTCTAGGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0042335 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

40.02

Weight (kDa)

5.41

Isoelectric Point (pI)

31.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 23 - 264 1.6e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04290 AT5G33370 AT5G33370
fragaria_vesca FvH4_6g01390 FvH4_6g01390
malus_domestica MD04G1234300.v1.1 MD12G1252200.v1.1
prunus_persica Prupe.6G354100_v2.0.a1
pyrus_communis pycom04g20800
rosa_chinensis RchiOBHm_Chr3g0448981 RchiOBHm_Chr3g0449021
rosa_laevigata RLG00000025862
rosa_multiflora Rmu_sc0003116.1_g000011
rosa_roxburghii Rroxscaffold_6G00427140
rosa_rugosa Rorug02G0617400
rosa_samantha Rh3AG015900 Rh3BG016000 Rh3CG015000 Rh3DG016300
rosa_wichuraiana Rw3G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 485
AasI GACNNNNNNGTC 1 cut(s) 572
AccB1I GGYRCC 3 cut(s) 311, 526, 854
AccI GTMKAC 1 cut(s) 576
AccII CGCG 1 cut(s) 729
AciI CCGC 3 cut(s) 675, 732, 749
AclWI GGATC 5 cut(s) 179, 664, 920, 968, 981
AcoI YGGCCR 2 cut(s) 199, 892
AdeI CACNNNGTG 1 cut(s) 494
AfaI GTAC 7 cut(s) 161, 272, 401, 533, 617, 1043, 1066
AfiI CCNNNNNNNGG 5 cut(s) 147, 421, 703, 908, 988
AgsI TTSAA 2 cut(s) 68, 745
AjnI CCWGG 2 cut(s) 639, 850
AleI CACNNNNGTG 1 cut(s) 664
AluBI AGCT 4 cut(s) 286, 682, 721, 754
AluI AGCT 4 cut(s) 286, 682, 721, 754
Alw21I GWGCWC 2 cut(s) 713, 723
Alw26I GTCTC 2 cut(s) 549, 948
AlwI GGATC 5 cut(s) 179, 664, 920, 968, 981
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 4 cut(s) 199, 214, 792, 892
ApeKI GCWGC 4 cut(s) 23, 43, 751, 1015
ArsI GACNNNNNNTTYG 1 cut(s) 34
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 3 cut(s) 440, 792, 899
AsuC2I CCSGG 2 cut(s) 148, 149
AsuHPI GGTGA 2 cut(s) 130, 436
AsuII TTCGAA 1 cut(s) 935
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 2 cut(s) 440, 899
BaeGI GKGCMC 2 cut(s) 433, 947
BalI TGGCCA 1 cut(s) 894
BamHI GGATCC 1 cut(s) 973
BanI GGYRCC 3 cut(s) 311, 526, 854
BanII GRGCYC 3 cut(s) 475, 631, 723
BauI CACGAG 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 793
Bbv12I GWGCWC 2 cut(s) 713, 723
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 4 cut(s) 10, 30, 763, 1027
BccI CCATC 1 cut(s) 990
BceAI ACGGC 2 cut(s) 253, 514
BcgI CGANNNNNNTGC 2 cut(s) 808, 842
BciT130I CCWGG 2 cut(s) 641, 852
BclI TGATCA 2 cut(s) 135, 445
BcnI CCSGG 2 cut(s) 148, 149
BcoDI GTCTC 2 cut(s) 549, 948
BfaI CTAG 5 cut(s) 540, 623, 662, 755, 1089
BisI GCNGC 5 cut(s) 24, 44, 732, 752, 1016
BlsI GCNGC 5 cut(s) 25, 45, 733, 753, 1017
BmcAI AGTACT 2 cut(s) 401, 1066
Bme1390I CCNGG 4 cut(s) 148, 149, 641, 852
Bme18I GGWCC 2 cut(s) 440, 899
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 3 cut(s) 440, 792, 899
BmiI GGNNCC 7 cut(s) 313, 472, 528, 628, 793, 856, 975
BmrFI CCNGG 4 cut(s) 148, 149, 641, 852
BmrI ACTGGG 1 cut(s) 1038
BmuI ACTGGG 1 cut(s) 1038
BpiI GAAGAC 1 cut(s) 793
BpmI CTGGAG 1 cut(s) 416
Bpu10I CCTNAGC 1 cut(s) 282
Bpu14I TTCGAA 1 cut(s) 935
BpuEI CTTGAG 1 cut(s) 295
BpuMI CCSGG 2 cut(s) 148, 149
Bsa29I ATCGAT 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 338
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 5 cut(s) 72, 147, 850, 895, 1077
BsaXI ACNNNNNCTCC 4 cut(s) 462, 492, 569, 599
Bsc4I CCNNNNNNNGG 5 cut(s) 147, 421, 703, 908, 988
Bse118I RCCGGY 2 cut(s) 197, 201
Bse1I ACTGG 4 cut(s) 358, 361, 399, 1033
Bse3DI GCAATG 3 cut(s) 21, 263, 804
Bse8I GATNNNNATC 1 cut(s) 338
BseBI CCWGG 2 cut(s) 641, 852
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 5 cut(s) 72, 147, 850, 895, 1077
BseGI GGATG 1 cut(s) 982
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 5 cut(s) 147, 421, 703, 908, 988
BseMI GCAATG 3 cut(s) 21, 263, 804
BseMII CTCAG 2 cut(s) 296, 1075
BseNI ACTGG 4 cut(s) 358, 361, 399, 1033
BseSI GKGCMC 2 cut(s) 433, 947
BseX3I CGGCCG 1 cut(s) 199
BseXI GCAGC 4 cut(s) 10, 30, 763, 1027
BseYI CCCAGC 1 cut(s) 329
BsgI GTGCAG 1 cut(s) 1034
Bsh1236I CGCG 1 cut(s) 729
Bsh1285I CGRYCG 1 cut(s) 202
BshFI GGCC 4 cut(s) 201, 216, 794, 894
BshNI GGYRCC 3 cut(s) 311, 526, 854
BshVI ATCGAT 1 cut(s) 174
BsiEI CGRYCG 1 cut(s) 202
BsiHKAI GWGCWC 2 cut(s) 713, 723
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 3 cut(s) 148, 198, 202
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 5 cut(s) 147, 421, 703, 908, 988
BsmAI GTCTC 2 cut(s) 549, 948
BsmFI GGGAC 1 cut(s) 656
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 4 cut(s) 201, 216, 794, 894
Bso31I GGTCTC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 147
Bsp119I TTCGAA 1 cut(s) 935
Bsp1286I GDGCHC 6 cut(s) 433, 475, 631, 713, 723, 947
Bsp143I GATC 7 cut(s) 135, 171, 445, 656, 765, 912, 973
BspACI CCGC 3 cut(s) 675, 732, 749
BspANI GGCC 4 cut(s) 201, 216, 794, 894
BspCNI CTCAG 2 cut(s) 295, 1074
BspDI ATCGAT 1 cut(s) 174
BspFNI CGCG 1 cut(s) 729
BspLI GGNNCC 7 cut(s) 313, 472, 528, 628, 793, 856, 975
BspPI GGATC 5 cut(s) 179, 664, 920, 968, 981
BspQI GCTCTTC 1 cut(s) 746
BspT104I TTCGAA 1 cut(s) 935
BspT107I GGYRCC 3 cut(s) 311, 526, 854
BspTNI GGTCTC 1 cut(s) 549
BsrDI GCAATG 3 cut(s) 21, 263, 804
BsrFI RCCGGY 2 cut(s) 197, 201
BsrI ACTGG 4 cut(s) 358, 361, 399, 1033
BssAI RCCGGY 2 cut(s) 197, 201
BssECI CCNNGG 5 cut(s) 72, 147, 850, 895, 1077
BssMI GATC 7 cut(s) 135, 171, 445, 656, 765, 912, 973
BssSI CACGAG 1 cut(s) 78
BssT1I CCWWGG 2 cut(s) 895, 1077
Bst2BI CACGAG 1 cut(s) 78
Bst2UI CCWGG 2 cut(s) 641, 852
Bst6I CTCTTC 1 cut(s) 746
BstBI TTCGAA 1 cut(s) 935
BstC8I GCNNGC 1 cut(s) 684
BstDEI CTNAG 4 cut(s) 282, 387, 1061, 1095
BstF5I GGATG 1 cut(s) 982
BstFNI CGCG 1 cut(s) 729
BstHHI GCGC 1 cut(s) 731
BstKTI GATC 7 cut(s) 138, 174, 448, 659, 768, 915, 976
BstMAI GTCTC 2 cut(s) 549, 948
BstMBI GATC 7 cut(s) 135, 171, 445, 656, 765, 912, 973
BstMCI CGRYCG 1 cut(s) 202
BstMWI GCNNNNNNNGC 6 cut(s) 20, 32, 320, 683, 688, 1012
BstNI CCWGG 2 cut(s) 641, 852
BstNSI RCATGY 1 cut(s) 1048
BstSCI CCNGG 4 cut(s) 146, 147, 639, 850
BstSLI GKGCMC 2 cut(s) 433, 947
BstUI CGCG 1 cut(s) 729
BstV1I GCAGC 4 cut(s) 10, 30, 763, 1027
BstV2I GAAGAC 1 cut(s) 793
BstX2I RGATCY 1 cut(s) 973
BstYI RGATCY 1 cut(s) 973
BstZI CGGCCG 1 cut(s) 199
Bsu15I ATCGAT 1 cut(s) 174
BsuRI GGCC 4 cut(s) 201, 216, 794, 894
BsuTUI ATCGAT 1 cut(s) 174
BtsCI GGATG 1 cut(s) 982
BtsIMutI CAGTG 3 cut(s) 244, 351, 1026
Cac8I GCNNGC 1 cut(s) 684
CfoI GCGC 1 cut(s) 731
Cfr10I RCCGGY 2 cut(s) 197, 201
Cfr13I GGNCC 3 cut(s) 440, 792, 899
Cfr9I CCCGGG 1 cut(s) 147
ClaI ATCGAT 1 cut(s) 174
Csp6I GTAC 7 cut(s) 160, 271, 400, 532, 616, 1042, 1065
CviAII CATG 4 cut(s) 688, 832, 1045, 1054
CviQI GTAC 7 cut(s) 160, 271, 400, 532, 616, 1042, 1065
DdeI CTNAG 4 cut(s) 282, 387, 1061, 1095
DpnI GATC 7 cut(s) 137, 173, 447, 658, 767, 914, 975
DpnII GATC 7 cut(s) 135, 171, 445, 656, 765, 912, 973
DraIII CACNNNGTG 1 cut(s) 494
DrdI GACNNNNNNGTC 1 cut(s) 572
DseDI GACNNNNNNGTC 1 cut(s) 572
EaeI YGGCCR 2 cut(s) 199, 892
EagI CGGCCG 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 746
EarI CTCTTC 1 cut(s) 746
Ecl136II GAGCTC 1 cut(s) 721
EclXI CGGCCG 1 cut(s) 199
Eco130I CCWWGG 2 cut(s) 895, 1077
Eco24I GRGCYC 3 cut(s) 475, 631, 723
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 2 cut(s) 440, 899
Eco52I CGGCCG 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 721
Eco88I CYCGRG 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 721
EcoO109I RGGNCCY 1 cut(s) 899
EcoRII CCWGG 2 cut(s) 639, 850
EcoT14I CCWWGG 2 cut(s) 895, 1077
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 475, 631, 723
ErhI CCWWGG 2 cut(s) 895, 1077
FaeI CATG 4 cut(s) 691, 835, 1048, 1057
FaqI GGGAC 1 cut(s) 656
FatI CATG 4 cut(s) 687, 831, 1044, 1053
FauI CCCGC 2 cut(s) 682, 756
FbaI TGATCA 2 cut(s) 135, 445
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 5 cut(s) 24, 44, 732, 752, 1016
FokI GGATG 1 cut(s) 969
FriOI GRGCYC 3 cut(s) 475, 631, 723
Fsp4HI GCNGC 5 cut(s) 24, 44, 732, 752, 1016
FspBI CTAG 5 cut(s) 540, 623, 662, 755, 1089
GlaI GCGC 1 cut(s) 730
GluI GCNGC 5 cut(s) 24, 44, 732, 752, 1016
GsaI CCCAGC 1 cut(s) 333
GsuI CTGGAG 1 cut(s) 416
HaeIII GGCC 4 cut(s) 201, 216, 794, 894
HapII CCGG 3 cut(s) 148, 198, 202
HhaI GCGC 1 cut(s) 731
Hin1II CATG 4 cut(s) 691, 835, 1048, 1057
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 9 cut(s) 101, 155, 184, 242, 277, 339, 357, 381, 1057
HpaII CCGG 3 cut(s) 148, 198, 202
HphI GGTGA 2 cut(s) 130, 436
Hpy166II GTNNAC 4 cut(s) 112, 466, 511, 577
Hpy188I TCNGA 8 cut(s) 98, 256, 380, 440, 450, 589, 917, 991
Hpy8I GTNNAC 4 cut(s) 112, 466, 511, 577
Hpy99I CGWCG 2 cut(s) 575, 719
HpyAV CCTTC 2 cut(s) 988, 996
HpyCH4IV ACGT 2 cut(s) 570, 798
HpyCH4V TGCA 5 cut(s) 4, 829, 924, 1015, 1048
HpyF10VI GCNNNNNNNGC 6 cut(s) 20, 32, 320, 683, 688, 1012
HpyF3I CTNAG 4 cut(s) 282, 387, 1061, 1095
HpySE526I ACGT 2 cut(s) 570, 798
Hsp92II CATG 4 cut(s) 691, 835, 1048, 1057
HspAI GCGC 1 cut(s) 729
Ksp22I TGATCA 2 cut(s) 135, 445
Kzo9I GATC 7 cut(s) 135, 171, 445, 656, 765, 912, 973
LguI GCTCTTC 1 cut(s) 746
LmnI GCTCC 4 cut(s) 470, 626, 716, 726
Lsp1109I GCAGC 4 cut(s) 10, 30, 763, 1027
MaeI CTAG 5 cut(s) 540, 623, 662, 755, 1089
MaeII ACGT 2 cut(s) 570, 798
MaeIII GTNAC 6 cut(s) 49, 243, 497, 643, 845, 868
MalI GATC 7 cut(s) 137, 173, 447, 658, 767, 914, 975
MboI GATC 7 cut(s) 135, 171, 445, 656, 765, 912, 973
MboII GAAGA 3 cut(s) 79, 733, 793
MflI RGATCY 1 cut(s) 973
MhlI GDGCHC 6 cut(s) 433, 475, 631, 713, 723, 947
MlsI TGGCCA 1 cut(s) 894
MluNI TGGCCA 1 cut(s) 894
MlyI GAGTC 4 cut(s) 149, 178, 251, 286
MmeI TCCRAC 1 cut(s) 748
Mox20I TGGCCA 1 cut(s) 894
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 894
MseI TTAA 2 cut(s) 288, 369
MslI CAYNNNNRTG 1 cut(s) 664
Msp20I TGGCCA 1 cut(s) 894
MspI CCGG 3 cut(s) 148, 198, 202
MspR9I CCNGG 4 cut(s) 148, 149, 641, 852
Mva1269I GAATGC 1 cut(s) 83
MvaI CCWGG 2 cut(s) 641, 852
MvnI CGCG 1 cut(s) 729
MwoI GCNNNNNNNGC 6 cut(s) 20, 32, 320, 683, 688, 1012
NciI CCSGG 2 cut(s) 148, 149
NdeII GATC 7 cut(s) 135, 171, 445, 656, 765, 912, 973
NlaIII CATG 4 cut(s) 691, 835, 1048, 1057
NlaIV GGNNCC 7 cut(s) 313, 472, 528, 628, 793, 856, 975
NmeAIII GCCGAG 2 cut(s) 97, 703
NmuCI GTSAC 4 cut(s) 49, 243, 643, 845
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 1048
NspV TTCGAA 1 cut(s) 935
OliI CACNNNNGTG 1 cut(s) 664
PciSI GCTCTTC 1 cut(s) 746
PcsI WCGNNNNNNNCGW 1 cut(s) 795
PctI GAATGC 1 cut(s) 83
PfeI GAWTC 5 cut(s) 101, 339, 357, 381, 1057
PflFI GACNNNGTC 1 cut(s) 569
PkrI GCNGC 5 cut(s) 25, 45, 733, 753, 1017
PleI GAGTC 4 cut(s) 149, 178, 250, 285
PpsI GAGTC 4 cut(s) 149, 178, 250, 285
PpuMI RGGWCCY 1 cut(s) 899
PsiI TTATAA 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 723
Psp5II RGGWCCY 1 cut(s) 899
Psp6I CCWGG 2 cut(s) 639, 850
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 2 cut(s) 639, 850
PspN4I GGNNCC 7 cut(s) 313, 472, 528, 628, 793, 856, 975
PspPI GGNCC 3 cut(s) 440, 792, 899
PspPPI RGGWCCY 1 cut(s) 899
PsrI GAACNNNNNNTAC 2 cut(s) 503, 535
PsuI RGATCY 1 cut(s) 973
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 7 cut(s) 161, 272, 401, 533, 617, 1043, 1066
RsaNI GTAC 7 cut(s) 160, 271, 400, 532, 616, 1042, 1065
RseI CAYNNNNRTG 1 cut(s) 664
SacI GAGCTC 1 cut(s) 723
SapI GCTCTTC 1 cut(s) 746
SaqAI TTAA 2 cut(s) 288, 369
SatI GCNGC 5 cut(s) 24, 44, 732, 752, 1016
Sau3AI GATC 7 cut(s) 135, 171, 445, 656, 765, 912, 973
Sau96I GGNCC 3 cut(s) 440, 792, 899
ScaI AGTACT 2 cut(s) 401, 1066
SchI GAGTC 4 cut(s) 149, 178, 251, 286
ScrFI CCNGG 4 cut(s) 148, 149, 641, 852
SduI GDGCHC 6 cut(s) 433, 475, 631, 713, 723, 947
SfuI TTCGAA 1 cut(s) 935
SinI GGWCC 2 cut(s) 440, 899
SmaI CCCGGG 1 cut(s) 149
SmiMI CAYNNNNRTG 1 cut(s) 664
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
SrfI GCCCGGGC 1 cut(s) 149
SsiI CCGC 3 cut(s) 675, 732, 749
SspMI CTAG 5 cut(s) 540, 623, 662, 755, 1089
SstI GAGCTC 1 cut(s) 723
StyD4I CCNGG 4 cut(s) 146, 147, 639, 850
StyI CCWWGG 2 cut(s) 895, 1077
TaiI ACGT 2 cut(s) 573, 801
TaqI TCGA 5 cut(s) 174, 405, 717, 818, 935
TatI WGTACW 3 cut(s) 399, 1041, 1064
TauI GCSGC 1 cut(s) 734
TfiI GAWTC 5 cut(s) 101, 339, 357, 381, 1057
Tru1I TTAA 2 cut(s) 288, 369
Tru9I TTAA 2 cut(s) 288, 369
TscAI CASTG 3 cut(s) 244, 358, 1033
TseFI GTSAC 4 cut(s) 49, 243, 643, 845
TseI GCWGC 4 cut(s) 23, 43, 751, 1015
Tsp45I GTSAC 4 cut(s) 49, 243, 643, 845
TspDTI ATGAA 3 cut(s) 793, 829, 1070
TspGWI ACGGA 3 cut(s) 306, 483, 703
TspMI CCCGGG 1 cut(s) 147
TspRI CASTG 3 cut(s) 244, 358, 1033
Tth111I GACNNNGTC 1 cut(s) 569
VpaK11BI GGWCC 2 cut(s) 440, 899
XceI RCATGY 1 cut(s) 1048
XmaI CCCGGG 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 576
XspI CTAG 5 cut(s) 540, 623, 662, 755, 1089
ZrmI AGTACT 2 cut(s) 401, 1066
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.