MD12G1252200.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010703
Physical Location & Seq
Forward (+)
32155853 .. 32158936
3084 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1252200.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGCATACCATTTTTGTTTCTTGGGTTGTTCTAGGTCTGCTTGCTGCTTGTGCTGGCCGTCAAGCCGAGGCACGAGCTTTCTTTGTGTTCGGAGATTCATTGGTGGACAGCGGCAACAATGACTACTTGGCCACCACTGCCAGGGCTGACAACTACCCTTATGGCATAGATTATCCTACTCACCGACCCACCGGTCGTTTTTCGAATGGCCTTAACATACCAGATCTTATCAGCGAGCAAATTGGCTCAGAACCCACGTTGCCCTACTTGAGCCCACATCTCACTGGACAAAAACTACTCGTTGGTGCCAACTTTGCTTCTGCTGGGATTGGAATTCTCAATGACACTGGAATCCAGTTTCTCAACATAATCCGAATCTACAAACAACTGAAGTACTTTCAGCAATACCAGACTAGAGTGAATGCTTTAGTTGGACCTGAGCAGGCTCAGCGACTTGTCAACGAAGCACTTGTTCTGATTACCCTTGGAGGCAATGATTTTGTCAACAACTACTACTTGGTGCCCTTCTCTGCTAGATCTCGACAATTCTCTCTCCCGGACTATGTCGTCTACCTCATCTCCGAGTATCGCAAAGTTCTAGCGAGGCTGTATGAATTAGGAGCTCGTAAGGTTTTGGTGACAGGCACAGGGCCACTAGGGTGTGCTCCAGCAGAGTTGGCTCAGCGCAGCAGAAGAGGCGAATGTGCGGTGGAGCTGCAGCGAGCTGCTTCTTTATTTAACCCACAGCTAGTCGATATGATCAACAGCCTGAATGGCCAAATTGGCTCAGATGTATTTGTTGCTGCAAATGCATTTCAAATGCACATGGATTTTATCTCCAATCCTCAAGCTTATGGATTTGTGACTTCAAAGATAGCTTGCTGTGGCCAAGGACCCTACAATGGGATCGGACTCTGCACAATTGCCTCCAACTTGTGCCCAAATAGAGATTTGTACGCGTTTTGGGATGCATTCCATCCTTCTGAGAAGGCAAGCAGAATTATTGCGCAGCAAATCCTTACTGGGTCCAACAAGTACATGAATCCAATGAACCTCAGCACTATTTTGGCCTTGGACTCTAAGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0042335 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

363

Amino Acids

39.75

Weight (kDa)

8.1

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 339 6.6e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04290 AT5G33370 AT5G33370
fragaria_vesca FvH4_6g01390 FvH4_6g01390
malus_domestica MD04G1234300.v1.1 MD12G1252200.v1.1
prunus_persica Prupe.6G354100_v2.0.a1
pyrus_communis pycom04g20800
rosa_chinensis RchiOBHm_Chr3g0448981 RchiOBHm_Chr3g0449021
rosa_laevigata RLG00000025862
rosa_multiflora Rmu_sc0003116.1_g000011
rosa_roxburghii Rroxscaffold_6G00427140
rosa_rugosa Rorug02G0617400
rosa_samantha Rh3AG015900 Rh3BG016000 Rh3CG015000 Rh3DG016300
rosa_wichuraiana Rw3G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 192, 566
Acc16I TGCGCA 1 cut(s) 1008
AccB1I GGYRCC 2 cut(s) 305, 520
AccI GTMKAC 1 cut(s) 570
AccII CGCG 1 cut(s) 959
AciI CCGC 2 cut(s) 111, 707
AclWI GGATC 1 cut(s) 914
AcoI YGGCCR 4 cut(s) 55, 129, 775, 886
AcsI RAATTY 1 cut(s) 333
AcuI CTGAAG 1 cut(s) 410
AfaI GTAC 3 cut(s) 395, 956, 1037
AfiI CCNNNNNNNGG 3 cut(s) 141, 902, 903
AflIII ACRYGT 1 cut(s) 957
AgeI ACCGGT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 818, 870
AjnI CCWGG 1 cut(s) 140
AleI CACNNNNGTG 1 cut(s) 658
AluBI AGCT 7 cut(s) 77, 623, 715, 725, 748, 851, 878
AluI AGCT 7 cut(s) 77, 623, 715, 725, 748, 851, 878
Alw21I GWGCWC 2 cut(s) 625, 667
AlwI GGATC 1 cut(s) 914
AoxI GGCC 7 cut(s) 55, 129, 208, 650, 775, 886, 1068
ApeKI GCWGC 7 cut(s) 44, 687, 715, 718, 725, 803, 1009
ApoI RAATTY 1 cut(s) 333
AsiGI ACCGGT 1 cut(s) 191
AspLEI GCGC 2 cut(s) 687, 1009
AspS9I GGNCC 4 cut(s) 434, 650, 893, 1026
AsuC2I CCSGG 1 cut(s) 557
AsuHPI GGTGA 2 cut(s) 173, 649
AsuII TTCGAA 1 cut(s) 203
AvaII GGWCC 3 cut(s) 434, 893, 1026
BaeGI GKGCMC 2 cut(s) 525, 941
BalI TGGCCA 3 cut(s) 131, 777, 888
BanI GGYRCC 2 cut(s) 305, 520
BanII GRGCYC 2 cut(s) 275, 625
BauI CACGAG 1 cut(s) 72
Bbv12I GWGCWC 2 cut(s) 625, 667
BbvCI CCTCAGC 1 cut(s) 1055
BbvI GCAGC 7 cut(s) 31, 699, 702, 712, 730, 790, 1021
BccI CCATC 1 cut(s) 984
BceAI ACGGC 1 cut(s) 42
BciT130I CCWGG 1 cut(s) 142
BclI TGATCA 1 cut(s) 759
BcnI CCSGG 1 cut(s) 557
BfaI CTAG 7 cut(s) 32, 414, 534, 599, 656, 749, 1087
BfmI CTRYAG 1 cut(s) 716
BglI GCCNNNNNGGC 2 cut(s) 774, 783
BglII AGATCT 2 cut(s) 223, 536
BisI GCNGC 8 cut(s) 45, 112, 688, 716, 719, 726, 804, 1010
BlpI GCTNAGC 2 cut(s) 447, 681
BlsI GCNGC 8 cut(s) 46, 113, 689, 717, 720, 727, 805, 1011
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 2 cut(s) 142, 557
Bme18I GGWCC 3 cut(s) 434, 893, 1026
BmgT120I GGNCC 4 cut(s) 434, 650, 893, 1026
BmiI GGNNCC 4 cut(s) 307, 522, 895, 1027
BmrFI CCNGG 2 cut(s) 142, 557
BmrI ACTGGG 1 cut(s) 1032
BmsI GCATC 1 cut(s) 958
BmuI ACTGGG 1 cut(s) 1032
BpmI CTGGAG 1 cut(s) 651
Bpu10I CCTNAGC 2 cut(s) 438, 1055
Bpu1102I GCTNAGC 2 cut(s) 447, 681
Bpu14I TTCGAA 1 cut(s) 203
BpuEI CTTGAG 2 cut(s) 289, 831
BpuMI CCSGG 1 cut(s) 557
BsaJI CCNNGG 5 cut(s) 66, 141, 484, 889, 1071
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 902, 903
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 4 cut(s) 289, 352, 355, 1027
Bse3DI GCAATG 1 cut(s) 499
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 5 cut(s) 66, 141, 484, 889, 1071
BseGI GGATG 2 cut(s) 973, 976
BseLI CCNNNNNNNGG 3 cut(s) 141, 902, 903
BseMI GCAATG 1 cut(s) 499
BseMII CTCAG 7 cut(s) 261, 429, 461, 695, 801, 975, 1069
BseNI ACTGG 4 cut(s) 289, 352, 355, 1027
BseSI GKGCMC 2 cut(s) 525, 941
BseXI GCAGC 7 cut(s) 31, 699, 702, 712, 730, 790, 1021
BseYI CCCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 901
Bsh1236I CGCG 1 cut(s) 959
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 7 cut(s) 57, 131, 210, 652, 777, 888, 1070
BshNI GGYRCC 2 cut(s) 305, 520
BshTI ACCGGT 1 cut(s) 191
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 2 cut(s) 625, 667
BsiSI CCGG 2 cut(s) 192, 557
BslI CCNNNNNNNGG 3 cut(s) 141, 902, 903
BsmI GAATGC 2 cut(s) 427, 971
BsnI GGCC 7 cut(s) 57, 131, 210, 652, 777, 888, 1070
Bsp119I TTCGAA 1 cut(s) 203
Bsp1286I GDGCHC 5 cut(s) 275, 525, 625, 667, 941
Bsp143I GATC 4 cut(s) 223, 536, 759, 906
Bsp1720I GCTNAGC 2 cut(s) 447, 681
BspACI CCGC 2 cut(s) 111, 707
BspANI GGCC 7 cut(s) 57, 131, 210, 652, 777, 888, 1070
BspCNI CTCAG 7 cut(s) 260, 430, 460, 694, 800, 976, 1068
BspFNI CGCG 1 cut(s) 959
BspLI GGNNCC 4 cut(s) 307, 522, 895, 1027
BspMAI CTGCAG 1 cut(s) 720
BspPI GGATC 1 cut(s) 914
BspT104I TTCGAA 1 cut(s) 203
BspT107I GGYRCC 2 cut(s) 305, 520
BsrDI GCAATG 1 cut(s) 499
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 4 cut(s) 289, 352, 355, 1027
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 5 cut(s) 66, 141, 484, 889, 1071
BssMI GATC 4 cut(s) 223, 536, 759, 906
BssSI CACGAG 1 cut(s) 72
BssT1I CCWWGG 3 cut(s) 484, 889, 1071
Bst2BI CACGAG 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 688
BstBI TTCGAA 1 cut(s) 203
BstC8I GCNNGC 7 cut(s) 42, 55, 236, 444, 723, 880, 994
BstDEI CTNAG 8 cut(s) 247, 438, 447, 681, 787, 984, 1055, 1080
BstF5I GGATG 2 cut(s) 973, 976
BstFNI CGCG 1 cut(s) 959
BstHHI GCGC 2 cut(s) 687, 1009
BstKTI GATC 4 cut(s) 226, 539, 762, 909
BstMBI GATC 4 cut(s) 223, 536, 759, 906
BstMCI CGRYCG 1 cut(s) 196
BstMWI GCNNNNNNNGC 9 cut(s) 50, 137, 314, 448, 677, 696, 774, 783, 809
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 2 cut(s) 140, 555
BstSFI CTRYAG 1 cut(s) 716
BstSLI GKGCMC 2 cut(s) 525, 941
BstUI CGCG 1 cut(s) 959
BstV1I GCAGC 7 cut(s) 31, 699, 702, 712, 730, 790, 1021
BstX2I RGATCY 2 cut(s) 223, 536
BstYI RGATCY 2 cut(s) 223, 536
BsuRI GGCC 7 cut(s) 57, 131, 210, 652, 777, 888, 1070
BtsCI GGATG 2 cut(s) 973, 976
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 3 cut(s) 135, 282, 345
Cac8I GCNNGC 7 cut(s) 42, 55, 236, 444, 723, 880, 994
CfoI GCGC 2 cut(s) 687, 1009
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 4 cut(s) 434, 650, 893, 1026
Csp6I GTAC 3 cut(s) 394, 955, 1036
CspAI ACCGGT 1 cut(s) 191
CviAII CATG 2 cut(s) 826, 1039
CviQI GTAC 3 cut(s) 394, 955, 1036
DdeI CTNAG 8 cut(s) 247, 438, 447, 681, 787, 984, 1055, 1080
DpnI GATC 4 cut(s) 225, 538, 761, 908
DpnII GATC 4 cut(s) 223, 536, 759, 906
DrdI GACNNNNNNGTC 2 cut(s) 192, 566
DseDI GACNNNNNNGTC 2 cut(s) 192, 566
EaeI YGGCCR 4 cut(s) 55, 129, 775, 886
Eam1104I CTCTTC 1 cut(s) 688
EarI CTCTTC 1 cut(s) 688
Ecl136II GAGCTC 1 cut(s) 623
Eco130I CCWWGG 3 cut(s) 484, 889, 1071
Eco24I GRGCYC 2 cut(s) 275, 625
Eco47I GGWCC 3 cut(s) 434, 893, 1026
Eco53kI GAGCTC 1 cut(s) 623
Eco57I CTGAAG 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 893
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 3 cut(s) 484, 889, 1071
EcoT22I ATGCAT 3 cut(s) 6, 814, 973
EcoT38I GRGCYC 2 cut(s) 275, 625
ErhI CCWWGG 3 cut(s) 484, 889, 1071
FaeI CATG 2 cut(s) 829, 1042
FatI CATG 2 cut(s) 825, 1038
FbaI TGATCA 1 cut(s) 759
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 8 cut(s) 45, 112, 688, 716, 719, 726, 804, 1010
FokI GGATG 2 cut(s) 963, 980
FriOI GRGCYC 2 cut(s) 275, 625
Fsp4HI GCNGC 8 cut(s) 45, 112, 688, 716, 719, 726, 804, 1010
FspBI CTAG 7 cut(s) 32, 414, 534, 599, 656, 749, 1087
FspI TGCGCA 1 cut(s) 1008
GlaI GCGC 2 cut(s) 686, 1008
GluI GCNGC 8 cut(s) 45, 112, 688, 716, 719, 726, 804, 1010
GsaI CCCAGC 1 cut(s) 327
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 7 cut(s) 57, 131, 210, 652, 777, 888, 1070
HapII CCGG 2 cut(s) 192, 557
HhaI GCGC 2 cut(s) 687, 1009
Hin1II CATG 2 cut(s) 829, 1042
Hin6I GCGC 2 cut(s) 685, 1007
HinP1I GCGC 2 cut(s) 685, 1007
HincII GTYRAC 2 cut(s) 460, 505
HindII GTYRAC 2 cut(s) 460, 505
HindIII AAGCTT 1 cut(s) 849
HinfI GANTC 6 cut(s) 95, 351, 375, 912, 1042, 1076
HpaII CCGG 2 cut(s) 192, 557
HphI GGTGA 2 cut(s) 173, 649
Hpy166II GTNNAC 4 cut(s) 106, 460, 505, 571
Hpy188I TCNGA 8 cut(s) 92, 250, 374, 477, 583, 790, 911, 985
Hpy188III TCNNGA 1 cut(s) 540
Hpy8I GTNNAC 4 cut(s) 106, 460, 505, 571
HpyAV CCTTC 3 cut(s) 535, 982, 990
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 7 cut(s) 4, 718, 806, 812, 823, 918, 971
HpyF10VI GCNNNNNNNGC 9 cut(s) 50, 137, 314, 448, 677, 696, 774, 783, 809
HpyF3I CTNAG 8 cut(s) 247, 438, 447, 681, 787, 984, 1055, 1080
HpySE526I ACGT 1 cut(s) 257
Hsp92II CATG 2 cut(s) 829, 1042
HspAI GCGC 2 cut(s) 685, 1007
Ksp22I TGATCA 1 cut(s) 759
Kzo9I GATC 4 cut(s) 223, 536, 759, 906
LmnI GCTCC 3 cut(s) 620, 670, 712
Lsp1109I GCAGC 7 cut(s) 31, 699, 702, 712, 730, 790, 1021
LweI GCATC 1 cut(s) 958
MaeI CTAG 7 cut(s) 32, 414, 534, 599, 656, 749, 1087
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 2 cut(s) 637, 862
MalI GATC 4 cut(s) 225, 538, 761, 908
MboI GATC 4 cut(s) 223, 536, 759, 906
MboII GAAGA 1 cut(s) 705
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 2 cut(s) 223, 536
MhlI GDGCHC 5 cut(s) 275, 525, 625, 667, 941
MlsI TGGCCA 3 cut(s) 131, 777, 888
MluCI AATT 7 cut(s) 240, 333, 545, 614, 780, 921, 999
MluI ACGCGT 1 cut(s) 957
MluNI TGGCCA 3 cut(s) 131, 777, 888
MlyI GAGTC 2 cut(s) 906, 1070
MmeI TCCRAC 3 cut(s) 412, 954, 1053
MnlI CCTC 8 cut(s) 61, 482, 584, 597, 689, 855, 937, 1064
Mox20I TGGCCA 3 cut(s) 131, 777, 888
Mph1103I ATGCAT 3 cut(s) 6, 814, 973
MscI TGGCCA 3 cut(s) 131, 777, 888
MseI TTAA 2 cut(s) 213, 738
MslI CAYNNNNRTG 1 cut(s) 658
Msp20I TGGCCA 3 cut(s) 131, 777, 888
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 2 cut(s) 192, 557
MspR9I CCNGG 2 cut(s) 142, 557
MunI CAATTG 1 cut(s) 921
Mva1269I GAATGC 2 cut(s) 427, 971
MvaI CCWGG 1 cut(s) 142
MvnI CGCG 1 cut(s) 959
MwoI GCNNNNNNNGC 9 cut(s) 50, 137, 314, 448, 677, 696, 774, 783, 809
NciI CCSGG 1 cut(s) 557
NdeII GATC 4 cut(s) 223, 536, 759, 906
NlaIII CATG 2 cut(s) 829, 1042
NlaIV GGNNCC 4 cut(s) 307, 522, 895, 1027
NmeAIII GCCGAG 1 cut(s) 91
NmuCI GTSAC 2 cut(s) 637, 862
NsbI TGCGCA 1 cut(s) 1008
NsiI ATGCAT 3 cut(s) 6, 814, 973
NspV TTCGAA 1 cut(s) 203
OliI CACNNNNGTG 1 cut(s) 658
PctI GAATGC 2 cut(s) 427, 971
PfeI GAWTC 4 cut(s) 95, 351, 375, 1042
PflFI GACNNNGTC 1 cut(s) 563
PfoI TCCNGGA 1 cut(s) 555
PinAI ACCGGT 1 cut(s) 191
PkrI GCNGC 8 cut(s) 46, 113, 689, 717, 720, 727, 805, 1011
PleI GAGTC 2 cut(s) 906, 1070
PpsI GAGTC 2 cut(s) 906, 1070
PpuMI RGGWCCY 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 625
Psp5II RGGWCCY 1 cut(s) 893
Psp6I CCWGG 1 cut(s) 140
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 4 cut(s) 307, 522, 895, 1027
PspPI GGNCC 4 cut(s) 434, 650, 893, 1026
PspPPI RGGWCCY 1 cut(s) 893
PstI CTGCAG 1 cut(s) 720
PsuI RGATCY 2 cut(s) 223, 536
PsyI GACNNNGTC 1 cut(s) 563
RsaI GTAC 3 cut(s) 395, 956, 1037
RsaNI GTAC 3 cut(s) 394, 955, 1036
RseI CAYNNNNRTG 1 cut(s) 658
SacI GAGCTC 1 cut(s) 625
SaqAI TTAA 2 cut(s) 213, 738
SatI GCNGC 8 cut(s) 45, 112, 688, 716, 719, 726, 804, 1010
Sau3AI GATC 4 cut(s) 223, 536, 759, 906
Sau96I GGNCC 4 cut(s) 434, 650, 893, 1026
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 2 cut(s) 906, 1070
ScrFI CCNGG 2 cut(s) 142, 557
SduI GDGCHC 5 cut(s) 275, 525, 625, 667, 941
SfaNI GCATC 1 cut(s) 958
SfcI CTRYAG 1 cut(s) 716
SfuI TTCGAA 1 cut(s) 203
SinI GGWCC 3 cut(s) 434, 893, 1026
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 2 cut(s) 268, 846
SmoI CTYRAG 2 cut(s) 268, 846
Sse9I AATT 7 cut(s) 240, 333, 545, 614, 780, 921, 999
SsiI CCGC 2 cut(s) 111, 707
SspMI CTAG 7 cut(s) 32, 414, 534, 599, 656, 749, 1087
SstI GAGCTC 1 cut(s) 625
StyD4I CCNGG 2 cut(s) 140, 555
StyI CCWWGG 3 cut(s) 484, 889, 1071
TaiI ACGT 1 cut(s) 260
TaqI TCGA 3 cut(s) 203, 541, 753
TasI AATT 7 cut(s) 240, 333, 545, 614, 780, 921, 999
TatI WGTACW 2 cut(s) 393, 1035
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 4 cut(s) 95, 351, 375, 1042
Tru1I TTAA 2 cut(s) 213, 738
Tru9I TTAA 2 cut(s) 213, 738
TscAI CASTG 3 cut(s) 142, 289, 352
TseFI GTSAC 2 cut(s) 637, 862
TseI GCWGC 7 cut(s) 44, 687, 715, 718, 725, 803, 1009
Tsp45I GTSAC 2 cut(s) 637, 862
TspDTI ATGAA 4 cut(s) 87, 627, 1055, 1064
TspRI CASTG 3 cut(s) 142, 289, 352
Tth111I GACNNNGTC 1 cut(s) 563
VpaK11BI GGWCC 3 cut(s) 434, 893, 1026
XapI RAATTY 1 cut(s) 333
XmiI GTMKAC 1 cut(s) 570
XspI CTAG 7 cut(s) 32, 414, 534, 599, 656, 749, 1087
ZrmI AGTACT 1 cut(s) 395
Zsp2I ATGCAT 3 cut(s) 6, 814, 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.