AT3G05240

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014550
Physical Location & Seq
Reverse (-)
1493684 .. 1495381
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05240.1

Sequence Viewer

Length: 1698 bp
ATGATGAAGAAGCACTACAAACCAATTCTCTCTCAGCTAGAGAATTGCAGATCATTGGTGGAACTGAATCAACTCCATGGTTTAATGATCAAATCCTCAGTTATCAGAAACGTCATTCCTCTAAGCCGACTCATAGATTTCTGCACTACTTGTCCTGAGACTATGAATCTCAGCTACGCGAGATCAGTTTTCGAAAGTATCGATTGTCCAAGTGTTTATATATGGAACTCTATGATCAGAGGCTACTCAAACAGTCCAAATCCAGATAAAGCATTGATCTTTTACCAAGAAATGCTTCGAAAAGGTTATTCTCCGGATTATTTCACTTTTCCTTATGTTCTTAAGGCTTGTTCTGGGTTAAGGGATATTCAATTCGGGAGTTGTGTTCATGGGTTTGTTGTGAAAACTGGGTTTGAAGTGAATATGTATGTTTCTACTTGCTTGCTTCATATGTATATGTGTTGTGGAGAAGTGAATTATGGTTTGAGAGTTTTTGAGGATATTCCTCAGTGGAACGTTGTTGCTTGGGGAAGTTTGATTTCTGGTTTTGTGAATAACAATCGGTTTAGTGACGCTATTGAGGCGTTTAGGGAGATGCAGAGTAATGGTGTGAAGGCAAATGAGACAATAATGGTGGATCTTTTGGTTGCTTGTGGTAGATGTAAAGACATTGTGACTGGGAAATGGTTTCATGGGTTTCTTCAAGGATTAGGCTTTGATCCATATTTTCAGTCCAAAGTTGGATTCAATGTGATTTTGGCTACAAGTCTCATTGATATGTATGCTAAATGTGGAGATCTGAGAACTGCGAGGTATCTGTTCGATGGAATGCCTGAGAGAACCTTGGTTTCTTGGAACTCCATCATTACAGGGTATAGTCAGAACGGTGATGCAGAGGAAGCACTGTGTATGTTTTTGGATATGCTTGATTTGGGTATAGCTCCTGATAAAGTAACCTTTTTGAGTGTTATCAGAGCTTCCATGATCCAAGGATGTTCACAATTGGGACAATCGATTCACGCTTATGTATCGAAAACCGGCTTTGTGAAGGATGCTGCCATAGTTTGTGCACTTGTAAATATGTATGCTAAAACCGGTGATGCTGAGAGCGCAAAAAAAGCGTTTGAAGATCTGGAGAAGAAAGATACAATAGCTTGGACCGTTGTGATCATTGGGTTAGCCAGTCACGGCCACGGGAATGAAGCATTGAGTATATTCCAGAGGATGCAAGAGAAAGGTAATGCTACACCTGATGGAATCACATATCTCGGGGTCTTATATGCGTGTAGCCACATAGGTTTAGTTGAGGAAGGACAGAGATACTTTGCAGAAATGAGAGATCTTCACGGCTTAGAACCAACAGTTGAACACTATGGATGTATGGTTGATATCTTGAGCCGTGCTGGTCGTTTTGAAGAGGCAGAGAGATTGGTAAAGACTATGCCGGTAAAACCCAATGTTAATATATGGGGTGCATTGTTAAATGGTTGTGATATACATGAAAATCTCGAGCTCACTGATCGGATAAGATCCATGGTTGCTGAGCCAGAAGAACTTGGTAGCGGAATCTATGTTCTTCTATCTAATATATACGCCAAAGCTGGCCGATGGGCTGATGTTAAGCTAATTAGAGAATCCATGAAGAGCAAAAGGGTAGATAAAGTTTTAGGTCATAGTTCTGTTGAAACAATGTTCTAA

Protein Analysis

565

Amino Acids

63.29

Weight (kDa)

6.05

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 70 - 117 2.3e-10 PPR repeat family
PPR_2 PF13041 172 - 208 2.2e-07 PPR repeat family
PPR PF01535 174 - 204 3.3e-06 PPR repeat
PPR_2 PF13041 282 - 326 3.2e-11 PPR repeat family
PPR PF01535 283 - 313 5.4e-08 PPR repeat
PPR_2 PF13041 381 - 430 2.9e-06 PPR repeat family
PPR PF01535 385 - 413 1e-05 PPR repeat
E_motif PF20431 500 - 562 3.8e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014550)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05240
fragaria_vesca FvH4_6g05570
malus_domestica MD12G1221500.v1.1
prunus_persica Prupe.6G328300_v2.0.a1
pyrus_communis pycom12g20770
rosa_chinensis RchiOBHm_Chr3g0454351
rosa_laevigata RLG00000025451 RLG00000025452 RLG00000025458
rosa_multiflora Rmu_sc0000006.1_g000021 Rmu_sc0007209.1_g000013
rosa_roxburghii Rroxscaffold_165G00437180 Rroxscaffold_6G00425180
rosa_rugosa Rorug02G0654500
rosa_samantha Rh3BG060200 Rh3DG060600
rosa_wichuraiana Rw3G004500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 179
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 1 cut(s) 1563
AclI AACGTT 1 cut(s) 516
AclWI GGATC 4 cut(s) 645, 713, 979, 1524
AcoI YGGCCR 2 cut(s) 1189, 1603
AflII CTTAAG 1 cut(s) 341
AgeI ACCGGT 1 cut(s) 1094
AgsI TTSAA 8 cut(s) 371, 416, 704, 748, 1127, 1367, 1415, 1685
AjuI GAANNNNNNNTTGG 4 cut(s) 279, 311, 728, 760
AloI GAACNNNNNNTCC 2 cut(s) 1557, 1589
AluBI AGCT 8 cut(s) 37, 174, 941, 977, 1154, 1513, 1601, 1624
AluI AGCT 8 cut(s) 37, 174, 941, 977, 1154, 1513, 1601, 1624
Alw21I GWGCWC 2 cut(s) 1072, 1515
Alw26I GTCTC 3 cut(s) 152, 617, 773
Alw44I GTGCAC 1 cut(s) 1068
AlwI GGATC 4 cut(s) 645, 713, 979, 1524
Ama87I CYCGRG 2 cut(s) 1268, 1508
Aor13HI TCCGGA 1 cut(s) 313
AoxI GGCC 2 cut(s) 1189, 1603
ApaLI GTGCAC 1 cut(s) 1068
ApeKI GCWGC 1 cut(s) 1055
AsiGI ACCGGT 1 cut(s) 1094
Asp700I GAANNNNTTC 1 cut(s) 294
AspLEI GCGC 1 cut(s) 1112
AspS9I GGNCC 1 cut(s) 1158
AsuHPI GGTGA 2 cut(s) 899, 1109
AsuII TTCGAA 2 cut(s) 192, 298
AvaI CYCGRG 2 cut(s) 1268, 1508
AvaII GGWCC 1 cut(s) 1158
BaeGI GKGCMC 1 cut(s) 1072
BanII GRGCYC 1 cut(s) 1515
BarI GAAGNNNNNNTAC 1 cut(s) 31
Bbv12I GWGCWC 2 cut(s) 1072, 1515
BbvI GCAGC 1 cut(s) 1042
BccI CCATC 4 cut(s) 818, 869, 1247, 1602
BceAI ACGGC 3 cut(s) 1204, 1363, 1383
BclI TGATCA 3 cut(s) 87, 234, 1167
BcoDI GTCTC 3 cut(s) 152, 617, 773
BfaI CTAG 1 cut(s) 38
BfrI CTTAAG 1 cut(s) 341
BglII AGATCT 3 cut(s) 796, 1129, 1339
BisI GCNGC 1 cut(s) 1056
BlpI GCTNAGC 1 cut(s) 1542
BlsI GCNGC 1 cut(s) 1057
Bme18I GGWCC 1 cut(s) 1158
BmeT110I CYCGRG 2 cut(s) 1268, 1508
BmgT120I GGNCC 1 cut(s) 1158
BmrI ACTGGG 2 cut(s) 417, 687
BmsI GCATC 5 cut(s) 585, 880, 1042, 1090, 1215
BmuI ACTGGG 2 cut(s) 417, 687
BpmI CTGGAG 1 cut(s) 1154
Bpu1102I GCTNAGC 1 cut(s) 1542
Bpu14I TTCGAA 2 cut(s) 192, 298
BpuEI CTTGAG 1 cut(s) 1414
Bsa29I ATCGAT 2 cut(s) 201, 1013
BsaJI CCNNGG 5 cut(s) 76, 843, 988, 1192, 1533
BsaWI WCCGGW 2 cut(s) 313, 1094
Bse118I RCCGGY 3 cut(s) 1037, 1094, 1444
Bse1I ACTGG 3 cut(s) 412, 682, 1182
BseAI TCCGGA 1 cut(s) 313
BseCI ATCGAT 2 cut(s) 201, 1013
BseDI CCNNGG 5 cut(s) 76, 843, 988, 1192, 1533
BseGI GGATG 4 cut(s) 998, 1057, 1230, 1382
BseMII CTCAG 9 cut(s) 47, 111, 147, 184, 521, 791, 825, 1095, 1533
BseNI ACTGG 3 cut(s) 412, 682, 1182
BseSI GKGCMC 1 cut(s) 1072
BseXI GCAGC 1 cut(s) 1042
BsgI GTGCAG 1 cut(s) 127
Bsh1236I CGCG 1 cut(s) 179
BshFI GGCC 2 cut(s) 1191, 1605
BshTI ACCGGT 1 cut(s) 1094
BshVI ATCGAT 2 cut(s) 201, 1013
BsiHKAI GWGCWC 2 cut(s) 1072, 1515
BsiHKCI CYCGRG 2 cut(s) 1268, 1508
BsiSI CCGG 4 cut(s) 314, 1038, 1095, 1445
BslFI GGGAC 1 cut(s) 1020
BsmAI GTCTC 3 cut(s) 152, 617, 773
BsmFI GGGAC 1 cut(s) 1020
BsmI GAATGC 1 cut(s) 834
BsnI GGCC 2 cut(s) 1191, 1605
BsoBI CYCGRG 2 cut(s) 1268, 1508
Bsp119I TTCGAA 2 cut(s) 192, 298
Bsp1286I GDGCHC 2 cut(s) 1072, 1515
Bsp13I TCCGGA 1 cut(s) 313
Bsp1720I GCTNAGC 1 cut(s) 1542
Bsp19I CCATGG 2 cut(s) 76, 1533
BspACI CCGC 1 cut(s) 1563
BspANI GGCC 2 cut(s) 1191, 1605
BspCNI CTCAG 9 cut(s) 46, 110, 148, 183, 520, 792, 826, 1096, 1534
BspDI ATCGAT 2 cut(s) 201, 1013
BspEI TCCGGA 1 cut(s) 313
BspFNI CGCG 1 cut(s) 179
BspPI GGATC 4 cut(s) 645, 713, 979, 1524
BspQI GCTCTTC 1 cut(s) 1637
BspT104I TTCGAA 2 cut(s) 192, 298
BspTI CTTAAG 1 cut(s) 341
BsrFI RCCGGY 3 cut(s) 1037, 1094, 1444
BsrI ACTGG 3 cut(s) 412, 682, 1182
BssAI RCCGGY 3 cut(s) 1037, 1094, 1444
BssECI CCNNGG 5 cut(s) 76, 843, 988, 1192, 1533
BssT1I CCWWGG 4 cut(s) 76, 843, 988, 1533
Bst4CI ACNGT 5 cut(s) 254, 887, 906, 1162, 1363
Bst6I CTCTTC 2 cut(s) 1410, 1637
BstAFI CTTAAG 1 cut(s) 341
BstBI TTCGAA 2 cut(s) 192, 298
BstC8I GCNNGC 2 cut(s) 443, 1603
BstDSI CCRYGG 3 cut(s) 76, 1192, 1533
BstF5I GGATG 4 cut(s) 998, 1057, 1230, 1382
BstFNI CGCG 1 cut(s) 179
BstHHI GCGC 1 cut(s) 1112
BstMAI GTCTC 3 cut(s) 152, 617, 773
BstMWI GCNNNNNNNGC 4 cut(s) 581, 899, 1109, 1118
BstSLI GKGCMC 1 cut(s) 1072
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 1 cut(s) 1042
BstX2I RGATCY 5 cut(s) 637, 796, 1129, 1339, 1529
BstYI RGATCY 5 cut(s) 637, 796, 1129, 1339, 1529
Bsu15I ATCGAT 2 cut(s) 201, 1013
BsuRI GGCC 2 cut(s) 1191, 1605
BsuTUI ATCGAT 2 cut(s) 201, 1013
BtgI CCRYGG 3 cut(s) 76, 1192, 1533
BtsCI GGATG 4 cut(s) 998, 1057, 1230, 1382
BtsIMutI CAGTG 3 cut(s) 515, 902, 1515
Cac8I GCNNGC 2 cut(s) 443, 1603
CfoI GCGC 1 cut(s) 1112
Cfr10I RCCGGY 3 cut(s) 1037, 1094, 1444
Cfr13I GGNCC 1 cut(s) 1158
ClaI ATCGAT 2 cut(s) 201, 1013
CseI GACGC 1 cut(s) 581
CspAI ACCGGT 1 cut(s) 1094
CspCI CAANNNNNGTGG 2 cut(s) 615, 650
CviAII CATG 7 cut(s) 77, 389, 692, 982, 1499, 1534, 1639
EaeI YGGCCR 2 cut(s) 1189, 1603
Eam1104I CTCTTC 2 cut(s) 1410, 1637
EarI CTCTTC 2 cut(s) 1410, 1637
Ecl136II GAGCTC 1 cut(s) 1513
Eco130I CCWWGG 4 cut(s) 76, 843, 988, 1533
Eco24I GRGCYC 1 cut(s) 1515
Eco32I GATATC 1 cut(s) 1390
Eco47I GGWCC 1 cut(s) 1158
Eco53kI GAGCTC 1 cut(s) 1513
Eco88I CYCGRG 2 cut(s) 1268, 1508
EcoICRI GAGCTC 1 cut(s) 1513
EcoRV GATATC 1 cut(s) 1390
EcoT14I CCWWGG 4 cut(s) 76, 843, 988, 1533
EcoT38I GRGCYC 1 cut(s) 1515
ErhI CCWWGG 4 cut(s) 76, 843, 988, 1533
FaeI CATG 7 cut(s) 80, 392, 695, 985, 1502, 1537, 1642
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FaqI GGGAC 1 cut(s) 1020
FatI CATG 7 cut(s) 76, 388, 691, 981, 1498, 1533, 1638
FauNDI CATATG 1 cut(s) 450
FbaI TGATCA 3 cut(s) 87, 234, 1167
Fnu4HI GCNGC 1 cut(s) 1056
FokI GGATG 4 cut(s) 1005, 1064, 1237, 1389
FriOI GRGCYC 1 cut(s) 1515
Fsp4HI GCNGC 1 cut(s) 1056
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 1111
GluI GCNGC 1 cut(s) 1056
GsuI CTGGAG 1 cut(s) 1154
HaeIII GGCC 2 cut(s) 1191, 1605
HapII CCGG 4 cut(s) 314, 1038, 1095, 1445
HgaI GACGC 1 cut(s) 581
HhaI GCGC 1 cut(s) 1112
Hin1II CATG 7 cut(s) 80, 392, 695, 985, 1502, 1537, 1642
Hin6I GCGC 1 cut(s) 1110
HinP1I GCGC 1 cut(s) 1110
HinfI GANTC 8 cut(s) 67, 129, 166, 744, 1015, 1257, 1566, 1634
HpaII CCGG 4 cut(s) 314, 1038, 1095, 1445
HphI GGTGA 2 cut(s) 899, 1109
Hpy166II GTNNAC 2 cut(s) 998, 1070
Hpy188I TCNGA 6 cut(s) 107, 239, 801, 882, 974, 1524
Hpy188III TCNNGA 9 cut(s) 155, 263, 314, 376, 944, 1133, 1219, 1393, 1508
Hpy8I GTNNAC 2 cut(s) 998, 1070
HpyAV CCTTC 3 cut(s) 607, 1042, 1304
HpyCH4III ACNGT 5 cut(s) 254, 887, 906, 1162, 1363
HpyCH4IV ACGT 2 cut(s) 111, 516
HpyCH4V TGCA 8 cut(s) 48, 144, 598, 893, 1070, 1228, 1328, 1475
HpyF10VI GCNNNNNNNGC 4 cut(s) 581, 899, 1109, 1118
HpySE526I ACGT 2 cut(s) 111, 516
Hsp92II CATG 7 cut(s) 80, 392, 695, 985, 1502, 1537, 1642
HspAI GCGC 1 cut(s) 1110
Kpn2I TCCGGA 1 cut(s) 313
Ksp22I TGATCA 3 cut(s) 87, 234, 1167
LguI GCTCTTC 1 cut(s) 1637
LmnI GCTCC 1 cut(s) 946
Lsp1109I GCAGC 1 cut(s) 1042
LweI GCATC 5 cut(s) 585, 880, 1042, 1090, 1215
MaeI CTAG 1 cut(s) 38
MaeII ACGT 2 cut(s) 111, 516
MaeIII GTNAC 4 cut(s) 569, 673, 952, 1184
MboII GAAGA 9 cut(s) 19, 692, 1139, 1150, 1334, 1427, 1562, 1568, 1654
MfeI CAATTG 1 cut(s) 1001
MflI RGATCY 5 cut(s) 637, 796, 1129, 1339, 1529
MhlI GDGCHC 2 cut(s) 1072, 1515
MluCI AATT 6 cut(s) 24, 43, 371, 475, 1001, 1626
MlyI GAGTC 1 cut(s) 123
MmeI TCCRAC 1 cut(s) 721
MroI TCCGGA 1 cut(s) 313
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 6 cut(s) 83, 342, 359, 1461, 1481, 1620
MslI CAYNNNNRTG 3 cut(s) 776, 1023, 1197
MspCI CTTAAG 1 cut(s) 341
MspI CCGG 4 cut(s) 314, 1038, 1095, 1445
MunI CAATTG 1 cut(s) 1001
Mva1269I GAATGC 1 cut(s) 834
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 4 cut(s) 581, 899, 1109, 1118
NcoI CCATGG 2 cut(s) 76, 1533
NdeI CATATG 1 cut(s) 450
NlaIII CATG 7 cut(s) 80, 392, 695, 985, 1502, 1537, 1642
NmuCI GTSAC 3 cut(s) 569, 673, 1184
NspV TTCGAA 2 cut(s) 192, 298
PaeR7I CTCGAG 1 cut(s) 1508
PciSI GCTCTTC 1 cut(s) 1637
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 834
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 7 cut(s) 67, 166, 744, 1015, 1257, 1566, 1634
PinAI ACCGGT 1 cut(s) 1094
PkrI GCNGC 1 cut(s) 1057
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
Psp124BI GAGCTC 1 cut(s) 1515
Psp1406I AACGTT 1 cut(s) 516
PspPI GGNCC 1 cut(s) 1158
PsuI RGATCY 5 cut(s) 637, 796, 1129, 1339, 1529
RseI CAYNNNNRTG 3 cut(s) 776, 1023, 1197
SacI GAGCTC 1 cut(s) 1515
SapI GCTCTTC 1 cut(s) 1637
SaqAI TTAA 6 cut(s) 83, 342, 359, 1461, 1481, 1620
SatI GCNGC 1 cut(s) 1056
Sau96I GGNCC 1 cut(s) 1158
SchI GAGTC 1 cut(s) 123
SduI GDGCHC 2 cut(s) 1072, 1515
SfaNI GCATC 5 cut(s) 585, 880, 1042, 1090, 1215
Sfr274I CTCGAG 1 cut(s) 1508
SfuI TTCGAA 2 cut(s) 192, 298
SinI GGWCC 1 cut(s) 1158
SlaI CTCGAG 1 cut(s) 1508
SmiMI CAYNNNNRTG 3 cut(s) 776, 1023, 1197
SmlI CTYRAG 3 cut(s) 341, 1393, 1508
SmoI CTYRAG 3 cut(s) 341, 1393, 1508
Sse9I AATT 6 cut(s) 24, 43, 371, 475, 1001, 1626
SsiI CCGC 1 cut(s) 1563
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 1 cut(s) 1515
StyI CCWWGG 4 cut(s) 76, 843, 988, 1533
TaaI ACNGT 5 cut(s) 254, 887, 906, 1162, 1363
TaiI ACGT 2 cut(s) 114, 519
TaqI TCGA 7 cut(s) 192, 201, 298, 822, 1013, 1031, 1509
TasI AATT 6 cut(s) 24, 43, 371, 475, 1001, 1626
TfiI GAWTC 7 cut(s) 67, 166, 744, 1015, 1257, 1566, 1634
Tru1I TTAA 6 cut(s) 83, 342, 359, 1461, 1481, 1620
Tru9I TTAA 6 cut(s) 83, 342, 359, 1461, 1481, 1620
TscAI CASTG 3 cut(s) 515, 909, 1522
TseFI GTSAC 3 cut(s) 569, 673, 1184
TseI GCWGC 1 cut(s) 1055
Tsp45I GTSAC 3 cut(s) 569, 673, 1184
TspDTI ATGAA 8 cut(s) 20, 179, 377, 437, 680, 1215, 1515, 1655
TspRI CASTG 3 cut(s) 515, 909, 1522
Vha464I CTTAAG 1 cut(s) 341
VneI GTGCAC 1 cut(s) 1068
VpaK11BI GGWCC 1 cut(s) 1158
XhoI CTCGAG 1 cut(s) 1508
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.