pycom12g20770

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014550
Physical Location & Seq
Forward (+)
22135753 .. 22137453
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g20770.1

Sequence Viewer

Length: 1701 bp
ATGAAGAAACACCAGAAAACCATTCTCTCACTCTTGGAAAAATGCAGAAGCATGAGTGAATTGAAACAAATACATGGGCTACGATCGATCACCACCTCAGTTGTTAACAATGTAATCCAGTTAAGTAGGCTTATTGATTTTTGTACCAATTCCGAGGCCAGAGACCTCGGCTACGCGAAGAATGTTTTCCGGCAGATCAGTCAGCCTAGTGTCTACATCTGGAACTCAATGATCAGAGGCTACTCCAATAGTGAGAACCCGGTTGAGTCTTTGATTATGTACAGGGAAATGCTGCAAAGGGGTTACTTGCCCGACAATTTTACTTTCCCGTTCGTGCTTAGATCGTGTTCTGTGATCGCTGATTACCGTTATGGAAAATGTGTTCATAACTGCATTGTGAAAACTGGGTATGGGTTGGAGGTCTATGGCTCTACTTGTTTGCTTAGAATGTATGCTTCCTGCGCAGATATGGAATCTGCACTCAAGGTGTTTGATATTATTCCCAAGTGGAATGTAGTTGCTTGGACTAGTTTGATTTCCGGGTTTGTTAATAACAGTCGGGCTATCGAGGCTATAAAGGTGTTCAAGGATATGGAACTTCACAATGTACAGCCTAATGAGATCACCATGGTGCATGTTGTAGTCGCTTGTGCACGAAGTAGGGATATTGATACAGGAAAGTTGGTTCACAGTCGTATTCGCCAACTAGGGTTTGATCCATTTGGTTCAAGTTTTAACTTTAATGTAGTTCTTGCAACTGCCTTATTAGATATGTACGCAAAATGTGGCAGCTTGAGAACTGCAAGGGATCTGTTCAACAAAATGGCTCAAAAAAACTTGGTTACCTGGAATTCAATGATTAGTGCTTATAATCAGTATGGCCGGGCTGAGGAGGCAATCAATCTCTTTTCTGATATGTGGATTGCTGGTTTTGTTCCAGACAAAACTACCTTTCTAGGTGTAATAGGTGCTTGTGCGCGGTTGGGGGCTTTGGCATTCGGACGAAGTCTTCATTCATATGTATCGAAAACTAGCATGGGCAAAGATACTACAATCGGGACTGCCTTAGTTGACATGTATGCTAAAATTGGAGATGCAGGAAGTGCTCGAAGAATTTTTGAGAACTTGCAGAAGAAAGATGTGATGGCATGGACTAGCATGATTATCTGTTTAGCTACGCATGGACACGGTATGGAAGCGCTACATGCATTTAGAAACATGCAAGAGGATGCTAGTGTTATTCCAGACCAGATCACTTACATTGGGGTTTTATCGGCATGTAGCCATATCGGTCTTGTAGAGGAGGGTCAGAGACATTTTGCCTCAATGGTAAATCTTTACGGCATAAAGCCGACTGTGGATCATTATTGCTGCATGGTTGATCTTCTGAGTCGAGCAGGCCGCTTTGATGAGGCGATGAGACTGCTGGAAGAGATGCCGATCCAACCCAATGTTGCTGTCTGGGGAGCTCTCTTGAACGGCTGCGAGATACATGAAAATGTTGATCTAGCTGACAAAGTGAGAAGATATATAACAGAGATGGAACCTCATGGCAGTGGAGTTTATGTTCTGCTGTCCAATATATACGCTAGGGCTGGTCGATGGCAGGAAGTAAATCGGGTTAGAGAACTGATGAAAAACAGGAATATTGCCAAAAATCTAGGGCATAGTTCGGTCGAAATGGAGTTGTTAAGTTCATAA

Protein Analysis

567

Amino Acids

63.64

Weight (kDa)

8.6

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 69 - 116 1.4e-08 PPR repeat family
PPR_2 PF13041 171 - 211 5.2e-09 PPR repeat family
PPR_2 PF13041 279 - 327 5.8e-10 PPR repeat family
PPR PF01535 281 - 310 4.4e-07 PPR repeat
PPR_1 PF12854 448 - 479 4.6e-07 PPR repeat
E_motif PF20431 498 - 560 1.3e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014550)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05240
fragaria_vesca FvH4_6g05570
malus_domestica MD12G1221500.v1.1
prunus_persica Prupe.6G328300_v2.0.a1
pyrus_communis pycom12g20770
rosa_chinensis RchiOBHm_Chr3g0454351
rosa_laevigata RLG00000025451 RLG00000025452 RLG00000025458
rosa_multiflora Rmu_sc0000006.1_g000021 Rmu_sc0007209.1_g000013
rosa_roxburghii Rroxscaffold_165G00437180 Rroxscaffold_6G00425180
rosa_rugosa Rorug02G0654500
rosa_samantha Rh3BG060200 Rh3DG060600
rosa_wichuraiana Rw3G004500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 870
Acc16I TGCGCA 1 cut(s) 463
AccI GTMKAC 1 cut(s) 213
AccII CGCG 2 cut(s) 176, 979
AciI CCGC 2 cut(s) 979, 1402
AclWI GGATC 4 cut(s) 710, 816, 1368, 1435
AcoI YGGCCR 1 cut(s) 880
AcsI RAATTY 2 cut(s) 850, 1113
AfaI GTAC 4 cut(s) 145, 281, 609, 776
AfeI AGCGCT 1 cut(s) 1200
AfiI CCNNNNNNNGG 1 cut(s) 889
AflIII ACRYGT 1 cut(s) 1074
AgsI TTSAA 6 cut(s) 64, 586, 729, 817, 855, 1477
AhlI ACTAGT 1 cut(s) 527
AjnI CCWGG 1 cut(s) 845
AleI CACNNNNGTG 1 cut(s) 629
AloI GAACNNNNNNTCC 6 cut(s) 366, 398, 669, 701, 1551, 1583
AluBI AGCT 4 cut(s) 792, 1175, 1469, 1511
AluI AGCT 4 cut(s) 792, 1175, 1469, 1511
Alw21I GWGCWC 3 cut(s) 655, 1108, 1471
Alw26I GTCTC 3 cut(s) 156, 1306, 1414
Alw44I GTGCAC 1 cut(s) 651
AlwI GGATC 4 cut(s) 710, 816, 1368, 1435
Aor51HI AGCGCT 1 cut(s) 1200
AoxI GGCC 3 cut(s) 156, 880, 1399
ApaLI GTGCAC 1 cut(s) 651
ApeKI GCWGC 4 cut(s) 292, 789, 1371, 1482
ApoI RAATTY 2 cut(s) 850, 1113
Asp700I GAANNNNTTC 1 cut(s) 185
AspLEI GCGC 3 cut(s) 464, 979, 1201
AsuC2I CCSGG 3 cut(s) 260, 541, 884
AsuHPI GGTGA 2 cut(s) 82, 616
BaeGI GKGCMC 1 cut(s) 655
BanII GRGCYC 1 cut(s) 1471
BbsI GAAGAC 1 cut(s) 1001
Bbv12I GWGCWC 3 cut(s) 655, 1108, 1471
BbvCI CCTCAGC 1 cut(s) 888
BbvI GCAGC 4 cut(s) 279, 801, 1358, 1469
BccI CCATC 3 cut(s) 1138, 1534, 1596
BceAI ACGGC 2 cut(s) 1357, 1495
BcgI CGANNNNNNTGC 2 cut(s) 1405, 1439
BciT130I CCWGG 1 cut(s) 847
BclI TGATCA 1 cut(s) 231
BcnI CCSGG 3 cut(s) 260, 541, 884
BcoDI GTCTC 3 cut(s) 156, 1306, 1414
BcuI ACTAGT 1 cut(s) 527
BfoI RGCGCY 1 cut(s) 1202
BisI GCNGC 5 cut(s) 293, 790, 1372, 1402, 1483
BlsI GCNGC 5 cut(s) 294, 791, 1373, 1403, 1484
Bme1390I CCNGG 4 cut(s) 260, 541, 847, 884
BmiI GGNNCC 1 cut(s) 1545
BmrFI CCNGG 4 cut(s) 260, 541, 847, 884
BmrI ACTGGG 1 cut(s) 414
BmsI GCATC 3 cut(s) 1084, 1219, 1425
BmuI ACTGGG 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 1001
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 467, 814
BpuMI CCSGG 3 cut(s) 260, 541, 884
Bsa29I ATCGAT 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 1439
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 3 cut(s) 153, 166, 627
BsaXI ACNNNNNCTCC 2 cut(s) 1551, 1581
Bsc4I CCNNNNNNNGG 1 cut(s) 889
Bse1I ACTGG 2 cut(s) 118, 409
Bse8I GATNNNNATC 1 cut(s) 1439
BseBI CCWGG 1 cut(s) 847
BseCI ATCGAT 1 cut(s) 86
BseDI CCNNGG 3 cut(s) 153, 166, 627
BseGI GGATG 1 cut(s) 1234
BseJI GATNNNNATC 1 cut(s) 1439
BseLI CCNNNNNNNGG 1 cut(s) 889
BseMII CTCAG 3 cut(s) 111, 879, 1379
BseNI ACTGG 2 cut(s) 118, 409
BseRI GAGGAG 2 cut(s) 905, 1316
BseSI GKGCMC 1 cut(s) 655
BseXI GCAGC 4 cut(s) 279, 801, 1358, 1469
BsgI GTGCAG 1 cut(s) 462
Bsh1236I CGCG 2 cut(s) 176, 979
Bsh1285I CGRYCG 2 cut(s) 86, 1677
BshFI GGCC 3 cut(s) 158, 882, 1401
BshVI ATCGAT 1 cut(s) 86
BsiEI CGRYCG 2 cut(s) 86, 1677
BsiHKAI GWGCWC 3 cut(s) 655, 1108, 1471
BsiSI CCGG 4 cut(s) 190, 260, 540, 883
BslFI GGGAC 1 cut(s) 1072
BslI CCNNNNNNNGG 1 cut(s) 889
BsmAI GTCTC 3 cut(s) 156, 1306, 1414
BsmFI GGGAC 1 cut(s) 1072
BsmI GAATGC 1 cut(s) 995
BsnI GGCC 3 cut(s) 158, 882, 1401
Bso31I GGTCTC 1 cut(s) 156
Bsp1286I GDGCHC 3 cut(s) 655, 1108, 1471
Bsp1407I TGTACA 2 cut(s) 279, 607
Bsp19I CCATGG 1 cut(s) 627
BspACI CCGC 2 cut(s) 979, 1402
BspANI GGCC 3 cut(s) 158, 882, 1401
BspCNI CTCAG 3 cut(s) 110, 880, 1380
BspDI ATCGAT 1 cut(s) 86
BspFNI CGCG 2 cut(s) 176, 979
BspLI GGNNCC 1 cut(s) 1545
BspPI GGATC 4 cut(s) 710, 816, 1368, 1435
BspTNI GGTCTC 1 cut(s) 156
BsrGI TGTACA 2 cut(s) 279, 607
BsrI ACTGG 2 cut(s) 118, 409
BssECI CCNNGG 3 cut(s) 153, 166, 627
BssT1I CCWWGG 1 cut(s) 627
Bst2UI CCWGG 1 cut(s) 847
Bst4CI ACNGT 5 cut(s) 368, 557, 692, 1190, 1357
Bst6I CTCTTC 1 cut(s) 1425
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstAUI TGTACA 2 cut(s) 279, 607
BstC8I GCNNGC 1 cut(s) 1399
BstDEI CTNAG 6 cut(s) 97, 338, 443, 888, 1066, 1388
BstDSI CCRYGG 1 cut(s) 627
BstEII GGTNACC 1 cut(s) 841
BstF5I GGATG 1 cut(s) 1234
BstFNI CGCG 2 cut(s) 176, 979
BstH2I RGCGCY 1 cut(s) 1202
BstHHI GCGC 3 cut(s) 464, 979, 1201
BstMAI GTCTC 3 cut(s) 156, 1306, 1414
BstMCI CGRYCG 2 cut(s) 86, 1677
BstMWI GCNNNNNNNGC 5 cut(s) 461, 569, 893, 1103, 1205
BstNI CCWGG 1 cut(s) 847
BstNSI RCATGY 5 cut(s) 638, 1078, 1208, 1222, 1281
BstPI GGTNACC 1 cut(s) 841
BstSCI CCNGG 4 cut(s) 258, 539, 845, 882
BstSLI GKGCMC 1 cut(s) 655
BstUI CGCG 2 cut(s) 176, 979
BstV1I GCAGC 4 cut(s) 279, 801, 1358, 1469
BstV2I GAAGAC 1 cut(s) 1001
BstX2I RGATCY 1 cut(s) 808
BstYI RGATCY 1 cut(s) 808
Bsu15I ATCGAT 1 cut(s) 86
BsuRI GGCC 3 cut(s) 158, 882, 1401
BsuTUI ATCGAT 1 cut(s) 86
BtgI CCRYGG 1 cut(s) 627
BtgZI GCGATG 1 cut(s) 1430
BtsCI GGATG 1 cut(s) 1234
BtsI GCAGTG 1 cut(s) 1561
BtsIMutI CAGTG 1 cut(s) 1561
Cac8I GCNNGC 1 cut(s) 1399
CfoI GCGC 3 cut(s) 464, 979, 1201
ClaI ATCGAT 1 cut(s) 86
Csp6I GTAC 4 cut(s) 144, 280, 608, 775
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviQI GTAC 4 cut(s) 144, 280, 608, 775
DdeI CTNAG 6 cut(s) 97, 338, 443, 888, 1066, 1388
EaeI YGGCCR 1 cut(s) 880
Eam1104I CTCTTC 1 cut(s) 1425
EarI CTCTTC 1 cut(s) 1425
Ecl136II GAGCTC 1 cut(s) 1469
Eco130I CCWWGG 1 cut(s) 627
Eco24I GRGCYC 1 cut(s) 1471
Eco31I GGTCTC 1 cut(s) 156
Eco47III AGCGCT 1 cut(s) 1200
Eco53kI GAGCTC 1 cut(s) 1469
Eco91I GGTNACC 1 cut(s) 841
EcoICRI GAGCTC 1 cut(s) 1469
EcoO65I GGTNACC 1 cut(s) 841
EcoRI GAATTC 1 cut(s) 850
EcoRII CCWGG 1 cut(s) 845
EcoT14I CCWWGG 1 cut(s) 627
EcoT22I ATGCAT 1 cut(s) 1210
EcoT38I GRGCYC 1 cut(s) 1471
ErhI CCWWGG 1 cut(s) 627
FaqI GGGAC 1 cut(s) 1072
FauNDI CATATG 1 cut(s) 1018
FbaI TGATCA 1 cut(s) 231
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 5 cut(s) 293, 790, 1372, 1402, 1483
FokI GGATG 1 cut(s) 1241
FriOI GRGCYC 1 cut(s) 1471
Fsp4HI GCNGC 5 cut(s) 293, 790, 1372, 1402, 1483
FspI TGCGCA 1 cut(s) 463
GlaI GCGC 3 cut(s) 463, 978, 1200
GluI GCNGC 5 cut(s) 293, 790, 1372, 1402, 1483
HaeII RGCGCY 1 cut(s) 1202
HaeIII GGCC 3 cut(s) 158, 882, 1401
HapII CCGG 4 cut(s) 190, 260, 540, 883
HhaI GCGC 3 cut(s) 464, 979, 1201
Hin6I GCGC 3 cut(s) 462, 977, 1199
HinP1I GCGC 3 cut(s) 462, 977, 1199
HincII GTYRAC 2 cut(s) 106, 1072
HindII GTYRAC 2 cut(s) 106, 1072
HinfI GANTC 3 cut(s) 266, 473, 1390
HpaI GTTAAC 1 cut(s) 106
HpaII CCGG 4 cut(s) 190, 260, 540, 883
HphI GGTGA 2 cut(s) 82, 616
Hpy166II GTNNAC 5 cut(s) 106, 214, 653, 688, 1072
Hpy188I TCNGA 6 cut(s) 154, 236, 913, 1001, 1311, 1389
Hpy188III TCNNGA 5 cut(s) 220, 938, 1057, 1244, 1474
Hpy8I GTNNAC 5 cut(s) 106, 214, 653, 688, 1072
HpyCH4III ACNGT 5 cut(s) 368, 557, 692, 1190, 1357
HpyF10VI GCNNNNNNNGC 5 cut(s) 461, 569, 893, 1103, 1205
HpyF3I CTNAG 6 cut(s) 97, 338, 443, 888, 1066, 1388
HspAI GCGC 3 cut(s) 462, 977, 1199
Ksp22I TGATCA 1 cut(s) 231
KspAI GTTAAC 1 cut(s) 106
LmnI GCTCC 1 cut(s) 1466
Lsp1109I GCAGC 4 cut(s) 279, 801, 1358, 1469
LweI GCATC 3 cut(s) 1084, 1219, 1425
MaeIII GTNAC 2 cut(s) 302, 841
MboII GAAGA 8 cut(s) 16, 190, 1001, 1122, 1144, 1376, 1442, 1536
MflI RGATCY 1 cut(s) 808
MhlI GDGCHC 3 cut(s) 655, 1108, 1471
MluCI AATT 6 cut(s) 59, 148, 316, 850, 1086, 1113
MlyI GAGTC 2 cut(s) 275, 1399
MmeI TCCRAC 2 cut(s) 396, 1468
Mph1103I ATGCAT 1 cut(s) 1210
MroXI GAANNNNTTC 1 cut(s) 185
MseI TTAA 6 cut(s) 105, 122, 549, 735, 741, 1691
MslI CAYNNNNRTG 4 cut(s) 629, 1017, 1497, 1554
MspI CCGG 4 cut(s) 190, 260, 540, 883
MspR9I CCNGG 4 cut(s) 260, 541, 847, 884
Mva1269I GAATGC 1 cut(s) 995
MvaI CCWGG 1 cut(s) 847
MvnI CGCG 2 cut(s) 176, 979
MwoI GCNNNNNNNGC 5 cut(s) 461, 569, 893, 1103, 1205
NciI CCSGG 3 cut(s) 260, 541, 884
NcoI CCATGG 1 cut(s) 627
NdeI CATATG 1 cut(s) 1018
NlaIV GGNNCC 1 cut(s) 1545
NmeAIII GCCGAG 1 cut(s) 147
NsbI TGCGCA 1 cut(s) 463
NsiI ATGCAT 1 cut(s) 1210
NspI RCATGY 5 cut(s) 638, 1078, 1208, 1222, 1281
OliI CACNNNNGTG 1 cut(s) 629
PciI ACATGT 1 cut(s) 1074
PctI GAATGC 1 cut(s) 995
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 1 cut(s) 473
PflFI GACNNNGTC 1 cut(s) 1005
PkrI GCNGC 5 cut(s) 294, 791, 1373, 1403, 1484
Ple19I CGATCG 1 cut(s) 86
PleI GAGTC 2 cut(s) 274, 1398
PpsI GAGTC 2 cut(s) 274, 1398
PscI ACATGT 1 cut(s) 1074
PsiI TTATAA 1 cut(s) 870
Psp124BI GAGCTC 1 cut(s) 1471
Psp6I CCWGG 1 cut(s) 845
PspEI GGTNACC 1 cut(s) 841
PspGI CCWGG 1 cut(s) 845
PspN4I GGNNCC 1 cut(s) 1545
PsuI RGATCY 1 cut(s) 808
PsyI GACNNNGTC 1 cut(s) 1005
PvuI CGATCG 1 cut(s) 86
RsaI GTAC 4 cut(s) 145, 281, 609, 776
RsaNI GTAC 4 cut(s) 144, 280, 608, 775
RseI CAYNNNNRTG 4 cut(s) 629, 1017, 1497, 1554
SacI GAGCTC 1 cut(s) 1471
SaqAI TTAA 6 cut(s) 105, 122, 549, 735, 741, 1691
SatI GCNGC 5 cut(s) 293, 790, 1372, 1402, 1483
SchI GAGTC 2 cut(s) 275, 1399
ScrFI CCNGG 4 cut(s) 260, 541, 847, 884
SduI GDGCHC 3 cut(s) 655, 1108, 1471
SfaNI GCATC 3 cut(s) 1084, 1219, 1425
SmiMI CAYNNNNRTG 4 cut(s) 629, 1017, 1497, 1554
SmlI CTYRAG 2 cut(s) 482, 793
SmoI CTYRAG 2 cut(s) 482, 793
SpeI ACTAGT 1 cut(s) 527
Sse9I AATT 6 cut(s) 59, 148, 316, 850, 1086, 1113
SsiI CCGC 2 cut(s) 979, 1402
SspI AATATT 1 cut(s) 1648
SstI GAGCTC 1 cut(s) 1471
StyD4I CCNGG 4 cut(s) 258, 539, 845, 882
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 5 cut(s) 368, 557, 692, 1190, 1357
TaqI TCGA 7 cut(s) 86, 567, 1025, 1108, 1393, 1600, 1677
TaqII GACCGA 2 cut(s) 1280, 1663
TasI AATT 6 cut(s) 59, 148, 316, 850, 1086, 1113
TatI WGTACW 2 cut(s) 279, 607
TauI GCSGC 1 cut(s) 1404
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 6 cut(s) 105, 122, 549, 735, 741, 1691
Tru9I TTAA 6 cut(s) 105, 122, 549, 735, 741, 1691
TscAI CASTG 1 cut(s) 1561
TseI GCWGC 4 cut(s) 292, 789, 1371, 1482
TspDTI ATGAA 7 cut(s) 17, 374, 1001, 1005, 1509, 1649, 1686
TspRI CASTG 1 cut(s) 1561
Tth111I GACNNNGTC 1 cut(s) 1005
VneI GTGCAC 1 cut(s) 651
XapI RAATTY 2 cut(s) 850, 1113
XceI RCATGY 5 cut(s) 638, 1078, 1208, 1222, 1281
XmiI GTMKAC 1 cut(s) 213
XmnI GAANNNNTTC 1 cut(s) 185
Zsp2I ATGCAT 1 cut(s) 1210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.