Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0014550

Physical Location & Seq

Genomic Coordinates Forward (+)

45841944 .. 45843754

Gene Structure 1811 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025458

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGAGTGAATTGAAAGAGATACATGGGATGATGATCACCACCTCAGTTGATAAGAATGTAATCCCATCGAGTAGGCTTATTGATTTCTGTGCTAATTCTGAAGCTGGAGACATCAGCTATGCCAAGTCGGTTTTTCGCCGAATTGAGCAACCTAGTGTCTATATCTGGAACTCAATGATCAGAGGGTGCTCGAATAGCGAGAACCCAGTTGAGTCTTTGATAATGTATAGAGAAATGGTGCATAGGGGTTACGCCCCTGACCATTTTACATTTCCATTTGTGCTCAAAGGGTGTTCGATAATCAGTGATGTTCATTATGGGAAATGTGTTCATAACTGCATTGTGAAAACTGGGTTTGAGTTGAATGTGTATGCCTCTTCTGGTTTACTCAATATGTACGGTTGTTGTGCAGATATGGAATCTGCACAGAAGGTGTTTGACAAAATGCCCAAGTGGAATGTAGTTGCTTGGACTAGTCTGATTTCTGGGTTTGTTGGGAACAATCAAGCTAGTGAGGCTATGAAGGTGTTTAAGGACATGGAACTTTGTGATGTCGAGCCTAATGAGATTACGATGGTCAATGTCTTAGTTGCTTGTGCTCGAAGTAGGGATATCAATACTGGGAAGTGGGTTCACAGTCGTATTCGCCAACTTGGGTTTGATCCCTTTGGATCGAGTTTTGGTTTTAATGTGATTCTTGCTACTGCCATTGTAGATATGTATGCAAAATGTGGCAGCTTGAGAACAGCAAGGCATCTGTTTGACAAGATGCCTGACAGAAACTTGGTTGCCTGGAATTCCATGATCAGTGCCTACAATCAATATGGCCAGGGTGATAAAGCGATTGATCTCTTTTTTGAAATGCAGATTGCTGGGTTTGTTCCGGACCAAGCTACCTTTCTGGGTGTGATTGGTTCTTGTGCACAGTTGGGGGCTTTGGCATTGGGACAAAGTCTTCATTCGTATGTATTAAAAACTAGCATAGGCACAGATGATTCTATTGGAACTGCTTTAGTGGACATGTATACGAAAATTGGAGATGCAGGCAGTGCTCGAACAATTTTTGACAATTTGCAGAAGAAAGATGTAATGGCATGGACTAGTATGATTGTTGGTTTAGCAATGCACGGGCATGCTGTGGAAGCACTGCATACTTTTAGAAGAATGCAAGAAGATGCTAGTGTTATTCCTGACCAGATCACTTATATTGGGGTTTTATGCGCGTGTAGCCATGTTGGGCTTGTGGAGGAGGGACAGAGACACTTTGGCTCAATGGTGGATGTTTATGGCGTAAAACCAACTGCAGAGCATTACGGCTGCATGGTTGATCTTCTGAGTCGAGCAGGCCGGTTTGAAGAGGCAGTGAAACTACTGGAAGAAATGCCAATTCAACCCAATGTTGCTATATGGGGAGCTCTCTTAAATGGGTGTGAGATATATGAAAATGTAGATCTGGCTGACCAAGTTAGAAGATACATCACAGAGTTGGAGCCTCATGTTAGTGGTGTCTTGGTCCTTCTTTCCAATATATATGCTAGGGCTGGAAGATGGCAAGAAGTAAAGCTAACTAGAGAATTGATGAAAGATAGGAGGATTGCCAAAAGTCTTGGATTTCTAATCCAGGGTGTAAAGCTTGCAATCAACCCGAAGTTCAAGAATGTCATTCTGGAGAGTGACTGCAAAGAACTTGTTGATGCCCTCAACAACAATATCAAGAGAGGCAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0097159 GO:1900864 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

576

Amino Acids

63.92

Weight (kDa)

6.13

Isoelectric Point (pI)

37.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	51 - 98	6.4e-09	PPR repeat family
PPR_2	PF13041	153 - 200	1.3e-09	PPR repeat family
PPR_2	PF13041	261 - 308	2.9e-09	PPR repeat family
PPR	PF01535	263 - 292	3.9e-07	PPR repeat
PPR_1	PF12854	430 - 461	5.3e-06	PPR repeat
E_motif	PF20431	480 - 535	3e-12	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014550)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05240
fragaria_vesca	FvH4_6g05570
malus_domestica	MD12G1221500.v1.1
prunus_persica	Prupe.6G328300_v2.0.a1
pyrus_communis	pycom12g20770
rosa_chinensis	RchiOBHm_Chr3g0454351
rosa_laevigata	RLG00000025451 RLG00000025452 RLG00000025458
rosa_multiflora	Rmu_sc0000006.1_g000021 Rmu_sc0007209.1_g000013
rosa_roxburghii	Rroxscaffold_165G00437180 Rroxscaffold_6G00425180
rosa_rugosa	Rorug02G0654500
rosa_samantha	Rh3BG060200 Rh3DG060600
rosa_wichuraiana	Rw3G004500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1025
AccII	CGCG	1 cut(s)	1223
AccIII	TCCGGA	1 cut(s)	883
AclWI	GGATC	2 cut(s)	656, 679
AcoI	YGGCCR	1 cut(s)	826
AcsI	RAATTY	1 cut(s)	796
AcuI	CTGAAG	1 cut(s)	120
AfaI	GTAC	1 cut(s)	398
AflIII	ACRYGT	1 cut(s)	1020
AgsI	TTSAA	6 cut(s)	13, 364, 860, 1355, 1391, 1654
AhlI	ACTAGT	2 cut(s)	473, 1100
AjnI	CCWGG	3 cut(s)	791, 828, 1620
AjuI	GAANNNNNNNTTGG	2 cut(s)	882, 914
AluBI	AGCT	8 cut(s)	104, 117, 509, 738, 893, 1415, 1564, 1633
AluI	AGCT	8 cut(s)	104, 117, 509, 738, 893, 1415, 1564, 1633
Alw21I	GWGCWC	6 cut(s)	191, 285, 601, 925, 1054, 1417
Alw26I	GTCTC	2 cut(s)	102, 1252
Alw44I	GTGCAC	1 cut(s)	921
AlwI	GGATC	2 cut(s)	656, 679
Aor13HI	TCCGGA	1 cut(s)	883
AoxI	GGCC	2 cut(s)	826, 1345
ApaLI	GTGCAC	1 cut(s)	921
ApeKI	GCWGC	2 cut(s)	735, 1317
ApoI	RAATTY	1 cut(s)	796
AspLEI	GCGC	1 cut(s)	1223
AspS9I	GGNCC	2 cut(s)	886, 1513
AsuHPI	GGTGA	2 cut(s)	28, 845
AvaII	GGWCC	2 cut(s)	886, 1513
BaeGI	GKGCMC	1 cut(s)	925
BalI	TGGCCA	1 cut(s)	828
BanII	GRGCYC	1 cut(s)	1417
BbsI	GAAGAC	1 cut(s)	947
Bbv12I	GWGCWC	6 cut(s)	191, 285, 601, 925, 1054, 1417
BbvI	GCAGC	2 cut(s)	747, 1304
BccI	CCATC	3 cut(s)	73, 568, 1542
BceAI	ACGGC	1 cut(s)	1330
BciT130I	CCWGG	3 cut(s)	793, 830, 1622
BclI	TGATCA	3 cut(s)	33, 177, 804
BcoDI	GTCTC	2 cut(s)	102, 1252
BcuI	ACTAGT	2 cut(s)	473, 1100
BfaI	CTAG	8 cut(s)	153, 474, 510, 978, 1101, 1179, 1536, 1569
BfmI	CTRYAG	1 cut(s)	1302
BglII	AGATCT	1 cut(s)	1450
BisI	GCNGC	2 cut(s)	736, 1318
BlsI	GCNGC	2 cut(s)	737, 1319
Bme1390I	CCNGG	3 cut(s)	793, 830, 1622
Bme18I	GGWCC	2 cut(s)	886, 1513
BmgT120I	GGNCC	2 cut(s)	886, 1513
BmiI	GGNNCC	1 cut(s)	1491
BmrFI	CCNGG	3 cut(s)	793, 830, 1622
BmrI	ACTGGG	3 cut(s)	200, 360, 630
BmsI	GCATC	5 cut(s)	759, 763, 1030, 1165, 1684
BmuI	ACTGGG	3 cut(s)	200, 360, 630
BpiI	GAAGAC	1 cut(s)	947
BpmI	CTGGAG	2 cut(s)	126, 1688
BpuEI	CTTGAG	1 cut(s)	760
BsaBI	GATNNNNATC	1 cut(s)	32
BsaJI	CCNNGG	2 cut(s)	829, 1621
BsaWI	WCCGGW	1 cut(s)	883
Bse118I	RCCGGY	1 cut(s)	1347
Bse1I	ACTGG	4 cut(s)	206, 355, 625, 1377
Bse3DI	GCAATG	1 cut(s)	1128
Bse8I	GATNNNNATC	1 cut(s)	32
BseAI	TCCGGA	1 cut(s)	883
BseBI	CCWGG	3 cut(s)	793, 830, 1622
BseDI	CCNNGG	2 cut(s)	829, 1621
BseGI	GGATG	2 cut(s)	33, 1285
BseJI	GATNNNNATC	1 cut(s)	32
BseMI	GCAATG	1 cut(s)	1128
BseMII	CTCAG	2 cut(s)	57, 1325
BseNI	ACTGG	4 cut(s)	206, 355, 625, 1377
BseRI	GAGGAG	1 cut(s)	1262
BseSI	GKGCMC	1 cut(s)	925
BseXI	GCAGC	2 cut(s)	747, 1304
BseYI	CCCAGC	1 cut(s)	872
BsgI	GTGCAG	2 cut(s)	408, 429
Bsh1236I	CGCG	1 cut(s)	1223
BshFI	GGCC	2 cut(s)	828, 1347
BsiHKAI	GWGCWC	6 cut(s)	191, 285, 601, 925, 1054, 1417
BsiSI	CCGG	2 cut(s)	884, 1348
BslFI	GGGAC	2 cut(s)	960, 1266
BsmAI	GTCTC	2 cut(s)	102, 1252
BsmFI	GGGAC	2 cut(s)	960, 1266
BsmI	GAATGC	1 cut(s)	1170
BsnI	GGCC	2 cut(s)	828, 1347
Bsp1286I	GDGCHC	6 cut(s)	191, 285, 601, 925, 1054, 1417
Bsp13I	TCCGGA	1 cut(s)	883
Bsp143I	GATC	9 cut(s)	33, 177, 661, 671, 804, 847, 1197, 1327, 1450
BspANI	GGCC	2 cut(s)	828, 1347
BspCNI	CTCAG	2 cut(s)	56, 1326
BspEI	TCCGGA	1 cut(s)	883
BspFNI	CGCG	1 cut(s)	1223
BspLI	GGNNCC	1 cut(s)	1491
BspMAI	CTGCAG	1 cut(s)	1306
BspPI	GGATC	2 cut(s)	656, 679
BsrDI	GCAATG	1 cut(s)	1128
BsrFI	RCCGGY	1 cut(s)	1347
BsrI	ACTGG	4 cut(s)	206, 355, 625, 1377
BssAI	RCCGGY	1 cut(s)	1347
BssECI	CCNNGG	2 cut(s)	829, 1621
BssMI	GATC	9 cut(s)	33, 177, 661, 671, 804, 847, 1197, 1327, 1450
BssNAI	GTATAC	1 cut(s)	1026
Bst1107I	GTATAC	1 cut(s)	1026
Bst2UI	CCWGG	3 cut(s)	793, 830, 1622
Bst4CI	ACNGT	3 cut(s)	401, 638, 927
Bst6I	CTCTTC	2 cut(s)	382, 1350
BstAPI	GCANNNNNTGC	1 cut(s)	1049
BstC8I	GCNNGC	4 cut(s)	1045, 1134, 1345, 1635
BstDEI	CTNAG	3 cut(s)	43, 586, 1334
BstF5I	GGATG	2 cut(s)	33, 1285
BstFNI	CGCG	1 cut(s)	1223
BstHHI	GCGC	1 cut(s)	1223
BstKTI	GATC	9 cut(s)	36, 180, 664, 674, 807, 850, 1200, 1330, 1453
BstMAI	GTCTC	2 cut(s)	102, 1252
BstMBI	GATC	9 cut(s)	33, 177, 661, 671, 804, 847, 1197, 1327, 1450
BstMWI	GCNNNNNNNGC	5 cut(s)	195, 515, 1049, 1142, 1227
BstNI	CCWGG	3 cut(s)	793, 830, 1622
BstNSI	RCATGY	2 cut(s)	1024, 1136
BstSCI	CCNGG	3 cut(s)	791, 828, 1620
BstSFI	CTRYAG	1 cut(s)	1302
BstSLI	GKGCMC	1 cut(s)	925
BstUI	CGCG	1 cut(s)	1223
BstV1I	GCAGC	2 cut(s)	747, 1304
BstV2I	GAAGAC	1 cut(s)	947
BstX2I	RGATCY	1 cut(s)	1450
BstYI	RGATCY	1 cut(s)	1450
BstZ17I	GTATAC	1 cut(s)	1026
BsuRI	GGCC	2 cut(s)	828, 1347
BtsCI	GGATG	2 cut(s)	33, 1285
BtsI	GCAGTG	3 cut(s)	1054, 1145, 1368
BtsIMutI	CAGTG	5 cut(s)	310, 814, 1054, 1145, 1368
Cac8I	GCNNGC	4 cut(s)	1045, 1134, 1345, 1635
CfoI	GCGC	1 cut(s)	1223
Cfr10I	RCCGGY	1 cut(s)	1347
Cfr13I	GGNCC	2 cut(s)	886, 1513
Csp6I	GTAC	1 cut(s)	397
CspCI	CAANNNNNGTGG	2 cut(s)	28, 63
CviAII	CATG	9 cut(s)	23, 538, 802, 1021, 1095, 1133, 1232, 1321, 1496
CviQI	GTAC	1 cut(s)	397
DdeI	CTNAG	3 cut(s)	43, 586, 1334
DpnI	GATC	9 cut(s)	35, 179, 663, 673, 806, 849, 1199, 1329, 1452
DpnII	GATC	9 cut(s)	33, 177, 661, 671, 804, 847, 1197, 1327, 1450
EaeI	YGGCCR	1 cut(s)	826
Eam1104I	CTCTTC	2 cut(s)	382, 1350
EarI	CTCTTC	2 cut(s)	382, 1350
Ecl136II	GAGCTC	1 cut(s)	1415
Eco24I	GRGCYC	1 cut(s)	1417
Eco32I	GATATC	1 cut(s)	613
Eco47I	GGWCC	2 cut(s)	886, 1513
Eco53kI	GAGCTC	1 cut(s)	1415
Eco57I	CTGAAG	1 cut(s)	120
EcoICRI	GAGCTC	1 cut(s)	1415
EcoRI	GAATTC	1 cut(s)	796
EcoRII	CCWGG	3 cut(s)	791, 828, 1620
EcoRV	GATATC	1 cut(s)	613
EcoT38I	GRGCYC	1 cut(s)	1417
FaeI	CATG	9 cut(s)	26, 541, 805, 1024, 1098, 1136, 1235, 1324, 1499
FaqI	GGGAC	2 cut(s)	960, 1266
FatI	CATG	9 cut(s)	22, 537, 801, 1020, 1094, 1132, 1231, 1320, 1495
FbaI	TGATCA	3 cut(s)	33, 177, 804
FblI	GTMKAC	1 cut(s)	1025
Fnu4HI	GCNGC	2 cut(s)	736, 1318
FokI	GGATG	2 cut(s)	40, 1292
FriOI	GRGCYC	1 cut(s)	1417
Fsp4HI	GCNGC	2 cut(s)	736, 1318
FspBI	CTAG	8 cut(s)	153, 474, 510, 978, 1101, 1179, 1536, 1569
GlaI	GCGC	1 cut(s)	1222
GluI	GCNGC	2 cut(s)	736, 1318
GsaI	CCCAGC	1 cut(s)	876
GsuI	CTGGAG	2 cut(s)	126, 1688
HaeIII	GGCC	2 cut(s)	828, 1347
HapII	CCGG	2 cut(s)	884, 1348
HhaI	GCGC	1 cut(s)	1223
Hin1II	CATG	9 cut(s)	26, 541, 805, 1024, 1098, 1136, 1235, 1324, 1499
Hin6I	GCGC	1 cut(s)	1221
HinP1I	GCGC	1 cut(s)	1221
HindIII	AAGCTT	1 cut(s)	1631
HinfI	GANTC	5 cut(s)	212, 419, 694, 995, 1336
HpaII	CCGG	2 cut(s)	884, 1348
HphI	GGTGA	2 cut(s)	28, 845
Hpy166II	GTNNAC	5 cut(s)	386, 634, 923, 1018, 1026
Hpy188I	TCNGA	4 cut(s)	100, 182, 480, 1335
Hpy188III	TCNNGA	6 cut(s)	166, 884, 1190, 1654, 1667, 1714
Hpy8I	GTNNAC	5 cut(s)	386, 634, 923, 1018, 1026
HpyAV	CCTTC	3 cut(s)	424, 517, 1526
HpyCH4III	ACNGT	3 cut(s)	401, 638, 927
HpyF10VI	GCNNNNNNNGC	5 cut(s)	195, 515, 1049, 1142, 1227
HpyF3I	CTNAG	3 cut(s)	43, 586, 1334
Hsp92II	CATG	9 cut(s)	26, 541, 805, 1024, 1098, 1136, 1235, 1324, 1499
HspAI	GCGC	1 cut(s)	1221
Kpn2I	TCCGGA	1 cut(s)	883
Ksp22I	TGATCA	3 cut(s)	33, 177, 804
Kzo9I	GATC	9 cut(s)	33, 177, 661, 671, 804, 847, 1197, 1327, 1450
LmnI	GCTCC	2 cut(s)	1412, 1489
Lsp1109I	GCAGC	2 cut(s)	747, 1304
LweI	GCATC	5 cut(s)	759, 763, 1030, 1165, 1684
MaeI	CTAG	8 cut(s)	153, 474, 510, 978, 1101, 1179, 1536, 1569
MaeIII	GTNAC	2 cut(s)	248, 1673
MalI	GATC	9 cut(s)	35, 179, 663, 673, 806, 849, 1199, 1329, 1452
MboI	GATC	9 cut(s)	33, 177, 661, 671, 804, 847, 1197, 1327, 1450
MflI	RGATCY	1 cut(s)	1450
MhlI	GDGCHC	6 cut(s)	191, 285, 601, 925, 1054, 1417
MlsI	TGGCCA	1 cut(s)	828
MluNI	TGGCCA	1 cut(s)	828
MlyI	GAGTC	2 cut(s)	221, 1345
MmeI	TCCRAC	1 cut(s)	1467
Mox20I	TGGCCA	1 cut(s)	828
MroI	TCCGGA	1 cut(s)	883
MscI	TGGCCA	1 cut(s)	828
MseI	TTAA	4 cut(s)	531, 687, 971, 1421
MslI	CAYNNNNRTG	3 cut(s)	963, 1131, 1500
Msp20I	TGGCCA	1 cut(s)	828
MspI	CCGG	2 cut(s)	884, 1348
MspR9I	CCNGG	3 cut(s)	793, 830, 1622
Mva1269I	GAATGC	1 cut(s)	1170
MvaI	CCWGG	3 cut(s)	793, 830, 1622
MvnI	CGCG	1 cut(s)	1223
MwoI	GCNNNNNNNGC	5 cut(s)	195, 515, 1049, 1142, 1227
NdeII	GATC	9 cut(s)	33, 177, 661, 671, 804, 847, 1197, 1327, 1450
NlaIII	CATG	9 cut(s)	26, 541, 805, 1024, 1098, 1136, 1235, 1324, 1499
NlaIV	GGNNCC	1 cut(s)	1491
NmuCI	GTSAC	1 cut(s)	1673
NspI	RCATGY	2 cut(s)	1024, 1136
PaeI	GCATGC	1 cut(s)	1136
PciI	ACATGT	1 cut(s)	1020
PctI	GAATGC	1 cut(s)	1170
PfeI	GAWTC	3 cut(s)	419, 694, 995
PflFI	GACNNNGTC	1 cut(s)	951
PkrI	GCNGC	2 cut(s)	737, 1319
PleI	GAGTC	2 cut(s)	220, 1344
PpsI	GAGTC	2 cut(s)	220, 1344
PscI	ACATGT	1 cut(s)	1020
Psp124BI	GAGCTC	1 cut(s)	1417
Psp6I	CCWGG	3 cut(s)	791, 828, 1620
PspFI	CCCAGC	1 cut(s)	872
PspGI	CCWGG	3 cut(s)	791, 828, 1620
PspN4I	GGNNCC	1 cut(s)	1491
PspPI	GGNCC	2 cut(s)	886, 1513
PstI	CTGCAG	1 cut(s)	1306
PsuI	RGATCY	1 cut(s)	1450
PsyI	GACNNNGTC	1 cut(s)	951
RsaI	GTAC	1 cut(s)	398
RsaNI	GTAC	1 cut(s)	397
RseI	CAYNNNNRTG	3 cut(s)	963, 1131, 1500
SacI	GAGCTC	1 cut(s)	1417
SaqAI	TTAA	4 cut(s)	531, 687, 971, 1421
SatI	GCNGC	2 cut(s)	736, 1318
Sau3AI	GATC	9 cut(s)	33, 177, 661, 671, 804, 847, 1197, 1327, 1450
Sau96I	GGNCC	2 cut(s)	886, 1513
SchI	GAGTC	2 cut(s)	221, 1345
ScrFI	CCNGG	3 cut(s)	793, 830, 1622
SduI	GDGCHC	6 cut(s)	191, 285, 601, 925, 1054, 1417
SfaNI	GCATC	5 cut(s)	759, 763, 1030, 1165, 1684
SfcI	CTRYAG	1 cut(s)	1302
SinI	GGWCC	2 cut(s)	886, 1513
SmiMI	CAYNNNNRTG	3 cut(s)	963, 1131, 1500
SmlI	CTYRAG	1 cut(s)	739
SmoI	CTYRAG	1 cut(s)	739
SpeI	ACTAGT	2 cut(s)	473, 1100
SphI	GCATGC	1 cut(s)	1136
SspMI	CTAG	8 cut(s)	153, 474, 510, 978, 1101, 1179, 1536, 1569
SstI	GAGCTC	1 cut(s)	1417
StyD4I	CCNGG	3 cut(s)	791, 828, 1620
TaaI	ACNGT	3 cut(s)	401, 638, 927
TaqI	TCGA	8 cut(s)	68, 191, 296, 555, 601, 674, 1054, 1339
TfiI	GAWTC	3 cut(s)	419, 694, 995
Tru1I	TTAA	4 cut(s)	531, 687, 971, 1421
Tru9I	TTAA	4 cut(s)	531, 687, 971, 1421
TscAI	CASTG	5 cut(s)	310, 814, 1054, 1152, 1368
TseFI	GTSAC	1 cut(s)	1673
TseI	GCWGC	2 cut(s)	735, 1317
Tsp45I	GTSAC	1 cut(s)	1673
TspDTI	ATGAA	6 cut(s)	302, 320, 536, 947, 1455, 1595
TspRI	CASTG	5 cut(s)	310, 814, 1054, 1152, 1368
Tth111I	GACNNNGTC	1 cut(s)	951
VneI	GTGCAC	1 cut(s)	921
VpaK11BI	GGWCC	2 cut(s)	886, 1513
XapI	RAATTY	1 cut(s)	796
XceI	RCATGY	2 cut(s)	1024, 1136
XmiI	GTMKAC	1 cut(s)	1025
XspI	CTAG	8 cut(s)	153, 474, 510, 978, 1101, 1179, 1536, 1569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.