AT3G05390

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012143
Physical Location & Seq
Reverse (-)
1546443 .. 1548679
2237 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05390.1

Sequence Viewer

Length: 1392 bp
ATGGGAAGAACATGTTTGCCGAGATGGCGATTAGGAAGACTAATGGCAGGATTACAAGTCATCTTAGGATTACTGGTGATCACTGTGAGCCTCTCATGTCTCTACCGATTCCATTCCGTGGGTTACTTCTTGCAAAACGAAGACACTTGCAACAATATCTACACAATCAAAGAAGTCAGCAGCGAAGGATTCGACCTAAAGGCGCTGCACGATCGTGTTGATGAAGTACTAGAAAAGATGGACAACTTGTACGAGAAGCTAGAGAAGACGGTAAAAGATATGGAGAAGAGCAAAGACGGAAGCAAAAAGGAGATGAAGAAGTTTCTTGAAGATGAAGTGATGAAGCCATTTTATTATGCTCATATCGGATTAAGGCAAATCCGGTTGCCAAAACCCGAAGGAATCAGAAACTCAACGGAAAAGGAAGAGCCTTTGATCAATAAGTTTCTCATTGAAGAGATAAGGCAGTACATTACACCAAAGGAGAATCGTGTTGGGAAGATAAACATGTTTGGAACAGAGAGAGTTTACAACACTATTGGTCATGCTTGTGCGTTGATGAAGATGGAGCTAGAGAAGTATATGGATTATGACGTTGGAGCGTATTGCGATGATGATTGGAACTTAGCACAGAAGCTGATGCTTAACGGGTGTGATCCGTTACCTAGAAGACGATGCTTGACAAGAGCATCAATGACTTACCAAAAACCTTATCCGATCAATGAGTCACTGTGGAAGCTTCCTGATGATAGAAATGTAAGATGGGGAAATTATCAATGCAGGAACTTCGCGTGCTTATCAAGCAAGAACCCGAAAAGGGGTTACACGAAATGCAGCGGATGTTTTGAAATGGAGAAAGAGAAAGATAAATGGGTCAAGAACAGTAGTTTGTTAGTTGATTTTATGATTGAAGATGTTCTGAGAGTGAAGCCAAGTGAGATCAGGATTGGATTGGATTATGGTGTTGGGACAGGAACATTTGCAGCAAGAATGAGAGAGAAGAATGTGACTATTGTCACAACAGCTTTGAATTTGGGAGCTCCTTTCAATGAGATGATTGCTTTGAGAGGTTTGATCCCATTGTACATATCTTTGAACCAGAGGCTACCGTTCTTCGATAATACAATGGATATGATTCACACTACCGGGTTAATGGATGGTTGGATCGATCTTCTTCTGATGGATTTCGTGTTATATGATTGGGACCGAGTGCTGAGACCGGGAGGGTTACTGTGGATTGATAGATTCTTCTGTAAAAAGAAGGATCTTGATGATTACATGTACATGTTCCTGCAGTTTAGGTACAAGAAACACAAGTGGGCTATTTCACCTAAATCCAAGGATGAGGTTTATCTCTCTGCATTGCTTGAGAAACCTCCTCGTGCAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

463

Amino Acids

54.26

Weight (kDa)

8.77

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 283 - 414 5.4e-07 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05390
fragaria_vesca FvH4_6g06250 FvH4_6g06250
malus_domestica MD12G1217000.v1.1
prunus_persica Prupe.6G323300_v2.0.a1
pyrus_communis pycom04g17960
rosa_chinensis RchiOBHm_Chr3g0455271
rosa_laevigata RLG00000025373
rosa_multiflora Rmu_sc0002169.1_g000037
rosa_roxburghii Rroxscaffold_6G00424410
rosa_rugosa Rorug03G0005500 Rorug03G0005600 Rorug03G0005700 Rorug03G0005800
rosa_samantha Rh3AG065600 Rh3BG067800 Rh3CG066200 Rh3DG067800
rosa_wichuraiana Rw3G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 118
AccII CGCG 1 cut(s) 791
AciI CCGC 1 cut(s) 837
AclWI GGATC 4 cut(s) 650, 1069, 1172, 1272
AcsI RAATTY 1 cut(s) 1030
AfaI GTAC 6 cut(s) 228, 251, 470, 1085, 1283, 1304
AfiI CCNNNNNNNGG 3 cut(s) 118, 817, 818
AflIII ACRYGT 4 cut(s) 11, 507, 1278, 1284
AgsI TTSAA 7 cut(s) 329, 455, 848, 911, 1030, 1048, 1096
AleI CACNNNNGTG 1 cut(s) 213
AluBI AGCT 6 cut(s) 259, 571, 637, 739, 1025, 1040
AluI AGCT 6 cut(s) 259, 571, 637, 739, 1025, 1040
Alw21I GWGCWC 1 cut(s) 1042
Alw26I GTCTC 2 cut(s) 104, 1210
AlwI GGATC 4 cut(s) 650, 1069, 1172, 1272
AlwNI CAGNNNCTG 1 cut(s) 637
ApeKI GCWGC 4 cut(s) 180, 205, 834, 983
ApoI RAATTY 1 cut(s) 1030
Asp700I GAANNNNTTC 1 cut(s) 915
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 1 cut(s) 1204
AsuC2I CCSGG 2 cut(s) 1147, 1221
AsuHPI GGTGA 2 cut(s) 88, 1320
AvaII GGWCC 1 cut(s) 1204
BanII GRGCYC 1 cut(s) 1042
BauI CACGAG 1 cut(s) 1380
BbsI GAAGAC 4 cut(s) 43, 147, 272, 676
Bbv12I GWGCWC 1 cut(s) 1042
BbvI GCAGC 4 cut(s) 192, 192, 846, 995
BccI CCATC 6 cut(s) 18, 232, 559, 756, 1151, 1174
BcgI CGANNNNNNTGC 2 cut(s) 769, 803
BclI TGATCA 2 cut(s) 78, 435
BcnI CCSGG 2 cut(s) 1147, 1221
BcoDI GTCTC 2 cut(s) 104, 1210
BfaI CTAG 4 cut(s) 230, 260, 572, 666
BfmI CTRYAG 1 cut(s) 1292
BfoI RGCGCY 1 cut(s) 206
BglI GCCNNNNNGGC 1 cut(s) 25
BisI GCNGC 4 cut(s) 181, 206, 835, 984
BlsI GCNGC 4 cut(s) 182, 207, 836, 985
BmcAI AGTACT 1 cut(s) 228
Bme1390I CCNGG 2 cut(s) 1147, 1221
Bme18I GGWCC 1 cut(s) 1204
BmgT120I GGNCC 1 cut(s) 1204
BmiI GGNNCC 1 cut(s) 1205
BmrFI CCNGG 2 cut(s) 1147, 1221
BmsI GCATC 3 cut(s) 630, 665, 698
BoxI GACNNNNGTC 1 cut(s) 1013
BpiI GAAGAC 4 cut(s) 43, 147, 272, 676
BpuEI CTTGAG 1 cut(s) 1388
BpuMI CCSGG 2 cut(s) 1147, 1221
Bsa29I ATCGAT 1 cut(s) 1167
BsaI GGTCTC 1 cut(s) 1210
BsaJI CCNNGG 2 cut(s) 117, 1338
BsaWI WCCGGW 1 cut(s) 381
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 817, 818
Bse1I ACTGG 1 cut(s) 78
Bse3DI GCAATG 1 cut(s) 1361
BseCI ATCGAT 1 cut(s) 1167
BseDI CCNNGG 2 cut(s) 117, 1338
BseGI GGATG 3 cut(s) 845, 1162, 1348
BseLI CCNNNNNNNGG 3 cut(s) 118, 817, 818
BseMI GCAATG 1 cut(s) 1361
BseMII CTCAG 2 cut(s) 911, 1205
BseNI ACTGG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 1368
BseXI GCAGC 4 cut(s) 192, 192, 846, 995
BsgI GTGCAG 1 cut(s) 191
Bsh1236I CGCG 1 cut(s) 791
Bsh1285I CGRYCG 1 cut(s) 214
BshVI ATCGAT 1 cut(s) 1167
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 1042
BsiSI CCGG 3 cut(s) 382, 1146, 1220
BslFI GGGAC 2 cut(s) 982, 1217
BslI CCNNNNNNNGG 3 cut(s) 118, 817, 818
BsmAI GTCTC 2 cut(s) 104, 1210
BsmFI GGGAC 2 cut(s) 982, 1217
Bso31I GGTCTC 1 cut(s) 1210
Bsp1286I GDGCHC 1 cut(s) 1042
Bsp1407I TGTACA 2 cut(s) 1083, 1281
BspACI CCGC 1 cut(s) 837
BspCNI CTCAG 2 cut(s) 912, 1206
BspDI ATCGAT 1 cut(s) 1167
BspFNI CGCG 1 cut(s) 791
BspLI GGNNCC 1 cut(s) 1205
BspMAI CTGCAG 1 cut(s) 1296
BspPI GGATC 4 cut(s) 650, 1069, 1172, 1272
BspQI GCTCTTC 2 cut(s) 281, 420
BspTNI GGTCTC 1 cut(s) 1210
BsrDI GCAATG 1 cut(s) 1361
BsrGI TGTACA 2 cut(s) 1083, 1281
BsrI ACTGG 1 cut(s) 78
BssECI CCNNGG 2 cut(s) 117, 1338
BssSI CACGAG 1 cut(s) 1380
BssT1I CCWWGG 1 cut(s) 1338
Bst2BI CACGAG 1 cut(s) 1380
Bst4CI ACNGT 6 cut(s) 85, 271, 732, 884, 1110, 1233
Bst6I CTCTTC 3 cut(s) 281, 420, 450
BstAUI TGTACA 2 cut(s) 1083, 1281
BstC8I GCNNGC 1 cut(s) 793
BstDEI CTNAG 4 cut(s) 64, 625, 920, 1214
BstDSI CCRYGG 1 cut(s) 117
BstF5I GGATG 3 cut(s) 845, 1162, 1348
BstFNI CGCG 1 cut(s) 791
BstH2I RGCGCY 1 cut(s) 206
BstHHI GCGC 1 cut(s) 205
BstMAI GTCTC 2 cut(s) 104, 1210
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 2 cut(s) 25, 801
BstNSI RCATGY 4 cut(s) 15, 511, 1282, 1288
BstPAI GACNNNNGTC 1 cut(s) 1013
BstSCI CCNGG 2 cut(s) 1145, 1219
BstSFI CTRYAG 1 cut(s) 1292
BstUI CGCG 1 cut(s) 791
BstV1I GCAGC 4 cut(s) 192, 192, 846, 995
BstV2I GAAGAC 4 cut(s) 43, 147, 272, 676
BstX2I RGATCY 1 cut(s) 1264
BstYI RGATCY 1 cut(s) 1264
Bsu15I ATCGAT 1 cut(s) 1167
BsuTUI ATCGAT 1 cut(s) 1167
BtgI CCRYGG 1 cut(s) 117
BtgZI GCGATG 1 cut(s) 624
BtsCI GGATG 3 cut(s) 845, 1162, 1348
BtsIMutI CAGTG 2 cut(s) 81, 728
Cac8I GCNNGC 1 cut(s) 793
CaiI CAGNNNCTG 1 cut(s) 637
CfoI GCGC 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 1204
ClaI ATCGAT 1 cut(s) 1167
Csp6I GTAC 6 cut(s) 227, 250, 469, 1084, 1282, 1303
CviAII CATG 6 cut(s) 12, 96, 508, 545, 1279, 1285
CviQI GTAC 6 cut(s) 227, 250, 469, 1084, 1282, 1303
DdeI CTNAG 4 cut(s) 64, 625, 920, 1214
Eam1104I CTCTTC 3 cut(s) 281, 420, 450
EarI CTCTTC 3 cut(s) 281, 420, 450
Ecl136II GAGCTC 1 cut(s) 1040
Eco130I CCWWGG 1 cut(s) 1338
Eco24I GRGCYC 1 cut(s) 1042
Eco31I GGTCTC 1 cut(s) 1210
Eco47I GGWCC 1 cut(s) 1204
Eco53kI GAGCTC 1 cut(s) 1040
EcoICRI GAGCTC 1 cut(s) 1040
EcoT14I CCWWGG 1 cut(s) 1338
EcoT38I GRGCYC 1 cut(s) 1042
ErhI CCWWGG 1 cut(s) 1338
FaeI CATG 6 cut(s) 15, 99, 511, 548, 1282, 1288
FaqI GGGAC 2 cut(s) 982, 1217
FatI CATG 6 cut(s) 11, 95, 507, 544, 1278, 1284
FbaI TGATCA 2 cut(s) 78, 435
Fnu4HI GCNGC 4 cut(s) 181, 206, 835, 984
FokI GGATG 3 cut(s) 852, 1169, 1355
FriOI GRGCYC 1 cut(s) 1042
Fsp4HI GCNGC 4 cut(s) 181, 206, 835, 984
FspBI CTAG 4 cut(s) 230, 260, 572, 666
GlaI GCGC 1 cut(s) 204
GluI GCNGC 4 cut(s) 181, 206, 835, 984
HaeII RGCGCY 1 cut(s) 206
HapII CCGG 3 cut(s) 382, 1146, 1220
HhaI GCGC 1 cut(s) 205
Hin1II CATG 6 cut(s) 15, 99, 511, 548, 1282, 1288
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 7 cut(s) 108, 189, 402, 487, 725, 1135, 1245
HpaII CCGG 3 cut(s) 382, 1146, 1220
HphI GGTGA 2 cut(s) 88, 1320
Hpy166II GTNNAC 1 cut(s) 529
Hpy188I TCNGA 5 cut(s) 368, 407, 717, 921, 1179
Hpy188III TCNNGA 5 cut(s) 326, 743, 877, 943, 1268
Hpy8I GTNNAC 1 cut(s) 529
HpyAV CCTTC 3 cut(s) 179, 392, 1255
HpyCH4III ACNGT 6 cut(s) 85, 271, 732, 884, 1110, 1233
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 9 cut(s) 133, 150, 208, 780, 834, 983, 1294, 1361, 1385
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 801
HpyF3I CTNAG 4 cut(s) 64, 625, 920, 1214
HpySE526I ACGT 1 cut(s) 594
Hsp92II CATG 6 cut(s) 15, 99, 511, 548, 1282, 1288
HspAI GCGC 1 cut(s) 203
Ksp22I TGATCA 2 cut(s) 78, 435
LguI GCTCTTC 2 cut(s) 281, 420
LmnI GCTCC 4 cut(s) 568, 599, 1037, 1045
Lsp1109I GCAGC 4 cut(s) 192, 192, 846, 995
LweI GCATC 3 cut(s) 630, 665, 698
MaeI CTAG 4 cut(s) 230, 260, 572, 666
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 7 cut(s) 122, 660, 726, 821, 1006, 1015, 1227
MflI RGATCY 1 cut(s) 1264
MhlI GDGCHC 1 cut(s) 1042
MluCI AATT 3 cut(s) 769, 1030, 1386
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 2 cut(s) 577, 1142
MnlI CCTC 7 cut(s) 101, 1061, 1095, 1217, 1339, 1386, 1389
MroXI GAANNNNTTC 1 cut(s) 915
MseI TTAA 4 cut(s) 371, 645, 1151, 1390
MslI CAYNNNNRTG 3 cut(s) 213, 549, 1283
MspA1I CMGCKG 1 cut(s) 837
MspI CCGG 3 cut(s) 382, 1146, 1220
MspR9I CCNGG 2 cut(s) 1147, 1221
MvnI CGCG 1 cut(s) 791
MwoI GCNNNNNNNGC 2 cut(s) 25, 801
NciI CCSGG 2 cut(s) 1147, 1221
NlaIII CATG 6 cut(s) 15, 99, 511, 548, 1282, 1288
NlaIV GGNNCC 1 cut(s) 1205
NmeAIII GCCGAG 1 cut(s) 45
NmuCI GTSAC 3 cut(s) 726, 1006, 1015
NspI RCATGY 4 cut(s) 15, 511, 1282, 1288
OliI CACNNNNGTG 1 cut(s) 213
PciI ACATGT 4 cut(s) 11, 507, 1278, 1284
PciSI GCTCTTC 2 cut(s) 281, 420
PdmI GAANNNNTTC 1 cut(s) 915
PfeI GAWTC 6 cut(s) 108, 189, 402, 487, 1135, 1245
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 4 cut(s) 182, 207, 836, 985
Ple19I CGATCG 1 cut(s) 214
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
PscI ACATGT 4 cut(s) 11, 507, 1278, 1284
PshAI GACNNNNGTC 1 cut(s) 1013
Psp124BI GAGCTC 1 cut(s) 1042
PspN4I GGNNCC 1 cut(s) 1205
PspPI GGNCC 1 cut(s) 1204
PstI CTGCAG 1 cut(s) 1296
PstNI CAGNNNCTG 1 cut(s) 637
PsuI RGATCY 1 cut(s) 1264
PvuI CGATCG 1 cut(s) 214
RsaI GTAC 6 cut(s) 228, 251, 470, 1085, 1283, 1304
RsaNI GTAC 6 cut(s) 227, 250, 469, 1084, 1282, 1303
RseI CAYNNNNRTG 3 cut(s) 213, 549, 1283
SacI GAGCTC 1 cut(s) 1042
SapI GCTCTTC 2 cut(s) 281, 420
SaqAI TTAA 4 cut(s) 371, 645, 1151, 1390
SatI GCNGC 4 cut(s) 181, 206, 835, 984
Sau96I GGNCC 1 cut(s) 1204
ScaI AGTACT 1 cut(s) 228
SchI GAGTC 1 cut(s) 734
ScrFI CCNGG 2 cut(s) 1147, 1221
SduI GDGCHC 1 cut(s) 1042
SfaNI GCATC 3 cut(s) 630, 665, 698
SfcI CTRYAG 1 cut(s) 1292
SinI GGWCC 1 cut(s) 1204
SmiMI CAYNNNNRTG 3 cut(s) 213, 549, 1283
SmlI CTYRAG 1 cut(s) 1367
SmoI CTYRAG 1 cut(s) 1367
Sse9I AATT 3 cut(s) 769, 1030, 1386
SsiI CCGC 1 cut(s) 837
SspMI CTAG 4 cut(s) 230, 260, 572, 666
SstI GAGCTC 1 cut(s) 1042
StyD4I CCNGG 2 cut(s) 1145, 1219
StyI CCWWGG 1 cut(s) 1338
TaaI ACNGT 6 cut(s) 85, 271, 732, 884, 1110, 1233
TaiI ACGT 1 cut(s) 597
TaqI TCGA 3 cut(s) 192, 1116, 1167
TaqII GACCGA 1 cut(s) 1221
TasI AATT 3 cut(s) 769, 1030, 1386
TatI WGTACW 4 cut(s) 226, 468, 1083, 1281
TfiI GAWTC 6 cut(s) 108, 189, 402, 487, 1135, 1245
Tru1I TTAA 4 cut(s) 371, 645, 1151, 1390
Tru9I TTAA 4 cut(s) 371, 645, 1151, 1390
TscAI CASTG 2 cut(s) 88, 735
TseFI GTSAC 3 cut(s) 726, 1006, 1015
TseI GCWGC 4 cut(s) 180, 205, 834, 983
Tsp45I GTSAC 3 cut(s) 726, 1006, 1015
TspDTI ATGAA 5 cut(s) 237, 329, 348, 356, 575
TspGWI ACGGA 4 cut(s) 106, 312, 431, 648
TspRI CASTG 2 cut(s) 88, 735
Van91I CCANNNNNTGG 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 1204
XapI RAATTY 1 cut(s) 1030
XceI RCATGY 4 cut(s) 15, 511, 1282, 1288
XmnI GAANNNNTTC 1 cut(s) 915
XspI CTAG 4 cut(s) 230, 260, 572, 666
ZrmI AGTACT 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.