Rw3G005170

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0012143
Physical Location & Seq
Reverse (-)
4326026 .. 4328830
2805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G005170.1

Sequence Viewer

Length: 1272 bp
ATGAAGACATATGCCGACATGTTTATGGGGTCAGATGAATATGGGGGCTTCGACATAAAAGCTCTGTCTGATCGAGTAGGGGAAGTGCTAAACAAATTGGATAGTTTACAAGAAAAGCTTGAGTCAGCTGTGCAGCAAATGGAGAAGAACAAAGATGTCTTGCATACAACTAATATCACAAAACTGGAGTTCAAGAAGTATCTGGAGGAGCAAGTAATCAGGCCTCTTTATAGTGCTCACATTTCTCTGAGACTGATTCGGTTACCTAACCCAGAAGGTATTAGAAACTCAACAATGAAAGAGGAACCTTTGATTAATACTTTTGTGATTGAAGAGATAAGGAAGTATATAACCCCAAAGGAGAGTAGAATTGGGAAGATTAATATATATGGTACAGAGAGGATATACAATACGATTGGACATGCTTGTGTGTTGTACAAGAAAGAGTTGGAAGAGTACATGAACTATGACATCGGGTCTTATTGTAACGATGATTGGAACTTGGCTCAGAAGCTGATGTTTAATGGTTGTGATCCTTTGCCGCGGAGGCGATGTTTGACAAGGGCCTCAAAGGTCTATCAGAAGCCTTATCCGATCAATGAGTCTCTGTGGAAATTGCCGGATGACAGAAATGTGCGGTGGAGCAATTATCAGTGCAGGAACTTTCAGTGCTTGATGAGCAATAATTCCAAGAGGGGCTATTCAAAGTGCGTAGGATGTTTTGAAATGGAGAAGGAAAAGCTTAAATGGATGATTACCAATAAATCGCTACCAGTTGATTTCCTGATCAGTGAAATTTTGGCTATTAAGCCTGGGGAGATAAGAATTGGTCTTGACTTTGGTGTTGGCACAGGAACTTATGCTGCAAGAATGAGAGAAAATAATGTTACGGTAGTCTCTACTGCTCTTAACCTTGGGGCTCCTTTCAATGAAATGATTGCATTAAGAGGTTTGGTTCCGCTGTATGTAACATTGAATCAGCGTCTACCTTTCTTTGATAATATAATGGACTTGATTCATACCGCTGGGTTTCTGGATGGCTGGATTGACCTGCAGTTGCTGGATTTCATCCTGTTTGATTGGGACCGAATTCTGAGGCCTGGCGGATTGTTATGGATTGACCGGTTCTTCTGCAATAAAAAGGATTTGGATGATTTCATGTATATGTTTCTGCAGTTGAGATACAAGAAACACAAGTGGGTTATTTCTCCCAAATCAAAGGATGAGGTCTACTTTTCTGCAGTGTTAGAGAAACCTCCAAGAGCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

423

Amino Acids

49.46

Weight (kDa)

8.64

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 266 - 372 2.3e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05390
fragaria_vesca FvH4_6g06250 FvH4_6g06250
malus_domestica MD12G1217000.v1.1
prunus_persica Prupe.6G323300_v2.0.a1
pyrus_communis pycom04g17960
rosa_chinensis RchiOBHm_Chr3g0455271
rosa_laevigata RLG00000025373
rosa_multiflora Rmu_sc0002169.1_g000037
rosa_roxburghii Rroxscaffold_6G00424410
rosa_rugosa Rorug03G0005500 Rorug03G0005600 Rorug03G0005700 Rorug03G0005800
rosa_samantha Rh3AG065600 Rh3BG067800 Rh3CG066200 Rh3DG067800
rosa_wichuraiana Rw3G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1059
AccI GTMKAC 2 cut(s) 985, 1230
AccII CGCG 1 cut(s) 544
AciI CCGC 6 cut(s) 542, 544, 637, 959, 1023, 1104
AclWI GGATC 1 cut(s) 527
AcsI RAATTY 2 cut(s) 795, 1089
AfaI GTAC 3 cut(s) 394, 437, 458
AflIII ACRYGT 1 cut(s) 18
AgeI ACCGGT 1 cut(s) 1122
AgsI TTSAA 6 cut(s) 193, 332, 705, 725, 928, 976
AhdI GACNNNNNGTC 1 cut(s) 475
AjnI CCWGG 2 cut(s) 811, 1099
AluBI AGCT 5 cut(s) 62, 118, 128, 514, 742
AluI AGCT 5 cut(s) 62, 118, 128, 514, 742
Alw21I GWGCWC 1 cut(s) 238
Alw26I GTCTC 3 cut(s) 244, 609, 901
AlwI GGATC 1 cut(s) 527
AlwNI CAGNNNCTG 2 cut(s) 514, 1060
AoxI GGCC 3 cut(s) 221, 564, 1097
ApeKI GCWGC 2 cut(s) 133, 863
ApoI RAATTY 2 cut(s) 795, 1089
AseI ATTAAT 2 cut(s) 315, 381
AsiGI ACCGGT 1 cut(s) 1122
AspS9I GGNCC 2 cut(s) 564, 1084
AvaII GGWCC 1 cut(s) 1084
BanII GRGCYC 1 cut(s) 922
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 238
BbvI GCAGC 2 cut(s) 145, 850
BccI CCATC 1 cut(s) 1031
BciT130I CCWGG 2 cut(s) 813, 1101
BclI TGATCA 1 cut(s) 786
BcoDI GTCTC 3 cut(s) 244, 609, 901
BfmI CTRYAG 3 cut(s) 1052, 1172, 1239
BfuAI ACCTGC 1 cut(s) 1059
BglI GCCNNNNNGGC 1 cut(s) 547
BisI GCNGC 3 cut(s) 134, 542, 864
BlsI GCNGC 3 cut(s) 135, 543, 865
Bme1390I CCNGG 2 cut(s) 813, 1101
Bme18I GGWCC 1 cut(s) 1084
BmeRI GACNNNNNGTC 1 cut(s) 475
BmgT120I GGNCC 2 cut(s) 564, 1084
BmiI GGNNCC 4 cut(s) 306, 921, 957, 1085
BmrFI CCNGG 2 cut(s) 813, 1101
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 2 cut(s) 206, 224
BpuEI CTTGAG 1 cut(s) 140
BsaJI CCNNGG 3 cut(s) 542, 812, 913
BsaWI WCCGGW 1 cut(s) 1122
BsaXI ACNNNNNCTCC 2 cut(s) 538, 568
Bse118I RCCGGY 1 cut(s) 1122
Bse1I ACTGG 2 cut(s) 189, 773
BseBI CCWGG 2 cut(s) 813, 1101
BseDI CCNNGG 3 cut(s) 542, 812, 913
BseGI GGATG 7 cut(s) 628, 722, 756, 1042, 1068, 1156, 1228
BseMII CTCAG 3 cut(s) 239, 521, 1085
BseNI ACTGG 2 cut(s) 189, 773
BseRI GAGGAG 1 cut(s) 221
BseXI GCAGC 2 cut(s) 145, 850
BseYI CCCAGC 1 cut(s) 1025
BsgI GTGCAG 2 cut(s) 152, 676
Bsh1236I CGCG 1 cut(s) 544
BshFI GGCC 3 cut(s) 223, 566, 1099
BshTI ACCGGT 1 cut(s) 1122
BsiHKAI GWGCWC 1 cut(s) 238
BsiSI CCGG 2 cut(s) 620, 1123
BslFI GGGAC 1 cut(s) 1097
BsmAI GTCTC 3 cut(s) 244, 609, 901
BsmFI GGGAC 1 cut(s) 1097
BsnI GGCC 3 cut(s) 223, 566, 1099
Bsp1286I GDGCHC 2 cut(s) 238, 922
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 4 cut(s) 70, 532, 594, 786
BspACI CCGC 6 cut(s) 542, 544, 637, 959, 1023, 1104
BspANI GGCC 3 cut(s) 223, 566, 1099
BspCNI CTCAG 3 cut(s) 240, 520, 1086
BspFNI CGCG 1 cut(s) 544
BspLI GGNNCC 4 cut(s) 306, 921, 957, 1085
BspMAI CTGCAG 3 cut(s) 1056, 1176, 1243
BspMI ACCTGC 1 cut(s) 1059
BspPI GGATC 1 cut(s) 527
BsrFI RCCGGY 1 cut(s) 1122
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 2 cut(s) 189, 773
BssAI RCCGGY 1 cut(s) 1122
BssECI CCNNGG 3 cut(s) 542, 812, 913
BssMI GATC 4 cut(s) 70, 532, 594, 786
BssT1I CCWWGG 1 cut(s) 913
Bst2UI CCWGG 2 cut(s) 813, 1101
Bst4CI ACNGT 1 cut(s) 892
Bst6I CTCTTC 2 cut(s) 327, 447
BstAUI TGTACA 1 cut(s) 435
BstDEI CTNAG 3 cut(s) 248, 507, 1094
BstDSI CCRYGG 1 cut(s) 542
BstEII GGTNACC 1 cut(s) 261
BstF5I GGATG 7 cut(s) 628, 722, 756, 1042, 1068, 1156, 1228
BstFNI CGCG 1 cut(s) 544
BstKTI GATC 4 cut(s) 73, 535, 597, 789
BstMAI GTCTC 3 cut(s) 244, 609, 901
BstMBI GATC 4 cut(s) 70, 532, 594, 786
BstMWI GCNNNNNNNGC 2 cut(s) 547, 678
BstNI CCWGG 2 cut(s) 813, 1101
BstNSI RCATGY 2 cut(s) 22, 425
BstPI GGTNACC 1 cut(s) 261
BstSCI CCNGG 2 cut(s) 811, 1099
BstSFI CTRYAG 3 cut(s) 1052, 1172, 1239
BstUI CGCG 1 cut(s) 544
BstV1I GCAGC 2 cut(s) 145, 850
BstV2I GAAGAC 1 cut(s) 11
BsuRI GGCC 3 cut(s) 223, 566, 1099
BtgI CCRYGG 1 cut(s) 542
BtgZI GCGATG 1 cut(s) 565
BtsCI GGATG 7 cut(s) 628, 722, 756, 1042, 1068, 1156, 1228
BtsI GCAGTG 1 cut(s) 1248
BtsIMutI CAGTG 4 cut(s) 659, 674, 796, 1248
BveI ACCTGC 1 cut(s) 1059
CaiI CAGNNNCTG 2 cut(s) 514, 1060
Cfr10I RCCGGY 1 cut(s) 1122
Cfr13I GGNCC 2 cut(s) 564, 1084
Cfr42I CCGCGG 1 cut(s) 545
CseI GACGC 1 cut(s) 971
Csp6I GTAC 3 cut(s) 393, 436, 457
CspAI ACCGGT 1 cut(s) 1122
CviAII CATG 4 cut(s) 19, 422, 460, 1159
CviQI GTAC 3 cut(s) 393, 436, 457
DdeI CTNAG 3 cut(s) 248, 507, 1094
DpnI GATC 4 cut(s) 72, 534, 596, 788
DpnII GATC 4 cut(s) 70, 532, 594, 786
DriI GACNNNNNGTC 1 cut(s) 475
Eam1104I CTCTTC 2 cut(s) 327, 447
Eam1105I GACNNNNNGTC 1 cut(s) 475
EarI CTCTTC 2 cut(s) 327, 447
EciI GGCGGA 1 cut(s) 1119
Eco130I CCWWGG 1 cut(s) 913
Eco147I AGGCCT 2 cut(s) 223, 1099
Eco24I GRGCYC 1 cut(s) 922
Eco47I GGWCC 1 cut(s) 1084
Eco91I GGTNACC 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 564
EcoO65I GGTNACC 1 cut(s) 261
EcoRI GAATTC 1 cut(s) 1089
EcoRII CCWGG 2 cut(s) 811, 1099
EcoT14I CCWWGG 1 cut(s) 913
EcoT38I GRGCYC 1 cut(s) 922
ErhI CCWWGG 1 cut(s) 913
FaeI CATG 4 cut(s) 22, 425, 463, 1162
FalI AAGNNNNNCTT 2 cut(s) 102, 134
FaqI GGGAC 1 cut(s) 1097
FatI CATG 4 cut(s) 18, 421, 459, 1158
FauNDI CATATG 1 cut(s) 10
FbaI TGATCA 1 cut(s) 786
FblI GTMKAC 2 cut(s) 985, 1230
Fnu4HI GCNGC 3 cut(s) 134, 542, 864
FokI GGATG 7 cut(s) 635, 729, 763, 1049, 1055, 1163, 1235
FriOI GRGCYC 1 cut(s) 922
Fsp4HI GCNGC 3 cut(s) 134, 542, 864
GluI GCNGC 3 cut(s) 134, 542, 864
GsaI CCCAGC 1 cut(s) 1029
GsuI CTGGAG 2 cut(s) 206, 224
HaeIII GGCC 3 cut(s) 223, 566, 1099
HapII CCGG 2 cut(s) 620, 1123
HgaI GACGC 1 cut(s) 971
Hin1II CATG 4 cut(s) 22, 425, 463, 1162
HindIII AAGCTT 2 cut(s) 116, 740
HinfI GANTC 5 cut(s) 122, 256, 602, 976, 1015
HpaII CCGG 2 cut(s) 620, 1123
Hpy166II GTNNAC 3 cut(s) 107, 986, 1231
Hpy188I TCNGA 8 cut(s) 34, 70, 249, 510, 582, 594, 1095, 1271
Hpy188III TCNNGA 5 cut(s) 193, 203, 784, 833, 1034
Hpy8I GTNNAC 3 cut(s) 107, 986, 1231
HpyAV CCTTC 2 cut(s) 269, 727
HpyCH4III ACNGT 1 cut(s) 892
HpyCH4V TGCA 9 cut(s) 133, 163, 657, 866, 941, 1054, 1134, 1174, 1241
HpyF10VI GCNNNNNNNGC 2 cut(s) 547, 678
HpyF3I CTNAG 3 cut(s) 248, 507, 1094
Hsp92II CATG 4 cut(s) 22, 425, 463, 1162
Ksp22I TGATCA 1 cut(s) 786
KspI CCGCGG 1 cut(s) 545
Kzo9I GATC 4 cut(s) 70, 532, 594, 786
LmnI GCTCC 3 cut(s) 208, 642, 925
Lsp1109I GCAGC 2 cut(s) 145, 850
MaeIII GTNAC 4 cut(s) 261, 485, 886, 967
MalI GATC 4 cut(s) 72, 534, 596, 788
MboI GATC 4 cut(s) 70, 532, 594, 786
MboII GAAGA 6 cut(s) 16, 157, 344, 388, 464, 1120
MhlI GDGCHC 2 cut(s) 238, 922
MluCI AATT 8 cut(s) 95, 369, 614, 646, 685, 795, 825, 1089
MlyI GAGTC 2 cut(s) 131, 611
MmeI TCCRAC 1 cut(s) 429
MseI TTAA 7 cut(s) 315, 381, 522, 744, 807, 909, 944
MslI CAYNNNNRTG 3 cut(s) 23, 426, 1163
MspA1I CMGCKG 4 cut(s) 128, 544, 961, 1025
MspI CCGG 2 cut(s) 620, 1123
MspR9I CCNGG 2 cut(s) 813, 1101
MvaI CCWGG 2 cut(s) 813, 1101
MvnI CGCG 1 cut(s) 544
MwoI GCNNNNNNNGC 2 cut(s) 547, 678
NdeI CATATG 1 cut(s) 10
NdeII GATC 4 cut(s) 70, 532, 594, 786
NlaIII CATG 4 cut(s) 22, 425, 463, 1162
NlaIV GGNNCC 4 cut(s) 306, 921, 957, 1085
NspI RCATGY 2 cut(s) 22, 425
PceI AGGCCT 2 cut(s) 223, 1099
PciI ACATGT 1 cut(s) 18
PfeI GAWTC 3 cut(s) 256, 976, 1015
PinAI ACCGGT 1 cut(s) 1122
PkrI GCNGC 3 cut(s) 135, 543, 865
PleI GAGTC 2 cut(s) 130, 610
PpsI GAGTC 2 cut(s) 130, 610
PscI ACATGT 1 cut(s) 18
PshBI ATTAAT 2 cut(s) 315, 381
Psp6I CCWGG 2 cut(s) 811, 1099
PspEI GGTNACC 1 cut(s) 261
PspFI CCCAGC 1 cut(s) 1025
PspGI CCWGG 2 cut(s) 811, 1099
PspN4I GGNNCC 4 cut(s) 306, 921, 957, 1085
PspPI GGNCC 2 cut(s) 564, 1084
PstI CTGCAG 3 cut(s) 1056, 1176, 1243
PstNI CAGNNNCTG 2 cut(s) 514, 1060
PvuII CAGCTG 1 cut(s) 128
RsaI GTAC 3 cut(s) 394, 437, 458
RsaNI GTAC 3 cut(s) 393, 436, 457
RseI CAYNNNNRTG 3 cut(s) 23, 426, 1163
SacII CCGCGG 1 cut(s) 545
SaqAI TTAA 7 cut(s) 315, 381, 522, 744, 807, 909, 944
SatI GCNGC 3 cut(s) 134, 542, 864
Sau3AI GATC 4 cut(s) 70, 532, 594, 786
Sau96I GGNCC 2 cut(s) 564, 1084
SchI GAGTC 2 cut(s) 131, 611
ScrFI CCNGG 2 cut(s) 813, 1101
SduI GDGCHC 2 cut(s) 238, 922
SfcI CTRYAG 3 cut(s) 1052, 1172, 1239
Sfr303I CCGCGG 1 cut(s) 545
SgrBI CCGCGG 1 cut(s) 545
SinI GGWCC 1 cut(s) 1084
SmiMI CAYNNNNRTG 3 cut(s) 23, 426, 1163
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 8 cut(s) 95, 369, 614, 646, 685, 795, 825, 1089
SseBI AGGCCT 2 cut(s) 223, 1099
SsiI CCGC 6 cut(s) 542, 544, 637, 959, 1023, 1104
StuI AGGCCT 2 cut(s) 223, 1099
StyD4I CCNGG 2 cut(s) 811, 1099
StyI CCWWGG 1 cut(s) 913
TaaI ACNGT 1 cut(s) 892
TaqI TCGA 2 cut(s) 51, 73
TaqII GACCGA 1 cut(s) 1101
TasI AATT 8 cut(s) 95, 369, 614, 646, 685, 795, 825, 1089
TatI WGTACW 2 cut(s) 435, 456
TauI GCSGC 1 cut(s) 544
TfiI GAWTC 3 cut(s) 256, 976, 1015
Tru1I TTAA 7 cut(s) 315, 381, 522, 744, 807, 909, 944
Tru9I TTAA 7 cut(s) 315, 381, 522, 744, 807, 909, 944
TscAI CASTG 4 cut(s) 659, 674, 796, 1248
TseI GCWGC 2 cut(s) 133, 863
TspDTI ATGAA 8 cut(s) 17, 51, 311, 476, 945, 1007, 1057, 1147
TspRI CASTG 4 cut(s) 659, 674, 796, 1248
VpaK11BI GGWCC 1 cut(s) 1084
VspI ATTAAT 2 cut(s) 315, 381
XapI RAATTY 2 cut(s) 795, 1089
XceI RCATGY 2 cut(s) 22, 425
XmiI GTMKAC 2 cut(s) 985, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.