MD12G1217000.v1.1

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012143
Physical Location & Seq
Forward (+)
29451634 .. 29454344
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1217000.v1.1.491

Sequence Viewer

Length: 1305 bp
ATGGGTTGGCTTCAGATGCTATTGGGAGGCCTTGTTATAATTTTCAGCATATGCAGTCTGTCCAAGTTCTACTCTGCAGGCGTTTTCCTTCACAATGAAGACATATGCCGACAATTTAATGGCGTCAAAGATGCGTACGAAGGTTTTGACATAAAAGCTTTGTCTGATCGAGTAGGGGAAGTTCTAAATAAACTAGAAAGCTTACAGGAAAAGCTCGAGTCAGCGGTGCAGCAAATTGAGAAGGACAAAGAGGCCTTGCACACAACTAACATTACTAAATTTGAGTACAAGAAGTATCTGGAGGAGGAAGTAATCAGGCCTCTTTATACCGCTCATATTTCTCTGAGGCTGATTCGGTTACCTAAAGCTGAAGGTATTCACAACTCAACGATGAAAGAGGAGCCTTTGATTAACACTTTTGTGATTGAAGAGATAAGGAAGTATATAACCCCAAAGGAGAGTAGAACCGGGAAGCTTAATATATATGGAACAGAAAGGATATACAATACAATCGGACATGCTTGTGTGTTATACAAGAAAGAGTTGGAAGAGTACATGAGCTATGACATCGGGTCTTATTGCAACGATGATTGGAACTTAGCTCAGAAGCTGATGCTTAACGGTTGTGATCCTTTGCCCCGGATGCGGTGCTTGACAAGGGCCTCCATGGTCTACCAGAAGCCTTACCCTATCAACGAGTCTCTGTGGAAACTTCCGGATGATAGAAATGTGCAGTGGAGCAATTATCATTGCACGAACTTTAAGTGCTTGATGGAAAATAACACCAAAAGGGGTTATTGCAAGTGTGTCGGATGTTTCGAAATGGAGAAGGAAAAGCTTAAATGGGTTACCAATAGCTCACTTACTGTAGATTTTCGGATCAGTGATGTTCTGGCTATTAAGCCTGGGGAGATGAGGATTGGTCTCGACTTTGGCATTGGCACAGGAACATTCGGCGCCTTTCAACGAGATGATTGCACCGAGAGGTTTGGTTCCATTGTTTATAACATTGAATCAATACCGTTCTTTGACAACACAATGGACATGATTCACACCGCTGGATTTCTGGACGGCTGGATTGACCTGCAACTGCTGGATTTTATCCTGTTTGATTGGGATCGAATTCTGAGGCCTGGCGGATTGTTATGGATTGACCGGTTCTTCTGTGTTAGAAAGGATTTGGATGACTTCATGTGCATGTTTCTGCAGCTGAGATACAAGAAACACAAGTGGGCTATTTCCCCTAAATCAAGGGATGAAGTCTACCTTTCTGCATTGTTGGAGAAACCTCCAAGAGCAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

435

Amino Acids

50.63

Weight (kDa)

6.3

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05390
fragaria_vesca FvH4_6g06250 FvH4_6g06250
malus_domestica MD12G1217000.v1.1
prunus_persica Prupe.6G323300_v2.0.a1
pyrus_communis pycom04g17960
rosa_chinensis RchiOBHm_Chr3g0455271
rosa_laevigata RLG00000025373
rosa_multiflora Rmu_sc0002169.1_g000037
rosa_roxburghii Rroxscaffold_6G00424410
rosa_rugosa Rorug03G0005500 Rorug03G0005600 Rorug03G0005700 Rorug03G0005800
rosa_samantha Rh3AG065600 Rh3BG067800 Rh3CG066200 Rh3DG067800
rosa_wichuraiana Rw3G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 38, 1005
Acc36I ACCTGC 1 cut(s) 1092
AccB1I GGYRCC 1 cut(s) 956
AccBSI CCGCTC 1 cut(s) 332
AccI GTMKAC 2 cut(s) 672, 1263
AccIII TCCGGA 1 cut(s) 715
AciI CCGC 5 cut(s) 224, 330, 646, 1056, 1137
AclWI GGATC 3 cut(s) 623, 887, 1125
AcsI RAATTY 2 cut(s) 278, 1122
AcuI CTGAAG 1 cut(s) 390
AcyI GRCGYC 2 cut(s) 123, 957
AfaI GTAC 3 cut(s) 137, 287, 554
AfiI CCNNNNNNNGG 1 cut(s) 645
AgeI ACCGGT 1 cut(s) 1155
AgsI TTSAA 3 cut(s) 428, 965, 1013
AhdI GACNNNNNGTC 1 cut(s) 571
AjnI CCWGG 2 cut(s) 904, 1132
AleI CACNNNNGTG 1 cut(s) 419
Alw26I GTCTC 2 cut(s) 705, 929
AlwI GGATC 3 cut(s) 623, 887, 1125
AlwNI CAGNNNCTG 1 cut(s) 610
Ama87I CYCGRG 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 715
AoxI GGCC 5 cut(s) 28, 252, 317, 660, 1130
ApeKI GCWGC 2 cut(s) 229, 1207
ApoI RAATTY 2 cut(s) 278, 1122
AsiGI ACCGGT 1 cut(s) 1155
Asp700I GAANNNNTTC 1 cut(s) 375
AspLEI GCGC 1 cut(s) 959
AspS9I GGNCC 1 cut(s) 660
AsuC2I CCSGG 2 cut(s) 469, 640
AsuII TTCGAA 1 cut(s) 819
AvaI CYCGRG 1 cut(s) 215
BanI GGYRCC 1 cut(s) 956
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 2 cut(s) 241, 1219
BccI CCATC 1 cut(s) 766
BceAI ACGGC 1 cut(s) 1087
BcgI CGANNNNNNTGC 4 cut(s) 790, 824, 957, 991
BciT130I CCWGG 2 cut(s) 906, 1134
BcnI CCSGG 2 cut(s) 469, 640
BcoDI GTCTC 2 cut(s) 705, 929
BfaI CTAG 1 cut(s) 194
BfmI CTRYAG 3 cut(s) 75, 867, 1205
BfoI RGCGCY 1 cut(s) 960
BfuAI ACCTGC 1 cut(s) 1092
BisI GCNGC 2 cut(s) 230, 1208
BlsI GCNGC 2 cut(s) 231, 1209
Bme1390I CCNGG 4 cut(s) 469, 640, 906, 1134
BmeRI GACNNNNNGTC 1 cut(s) 571
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 660
BmiI GGNNCC 3 cut(s) 402, 958, 994
BmrFI CCNGG 4 cut(s) 469, 640, 906, 1134
BmsI GCATC 4 cut(s) 6, 121, 603, 633
BpiI GAAGAC 1 cut(s) 105
BpmI CTGGAG 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 819
BpuMI CCSGG 2 cut(s) 469, 640
BsaBI GATNNNNATC 1 cut(s) 1116
BsaHI GRCGYC 2 cut(s) 123, 957
BsaI GGTCTC 1 cut(s) 929
BsaJI CCNNGG 3 cut(s) 638, 666, 905
BsaWI WCCGGW 2 cut(s) 715, 1155
BsaXI ACNNNNNCTCC 2 cut(s) 18, 48
Bsc4I CCNNNNNNNGG 1 cut(s) 645
Bse118I RCCGGY 1 cut(s) 1155
Bse3DI GCAATG 1 cut(s) 748
Bse8I GATNNNNATC 1 cut(s) 1116
BseAI TCCGGA 1 cut(s) 715
BseBI CCWGG 2 cut(s) 906, 1134
BseDI CCNNGG 3 cut(s) 638, 666, 905
BseGI GGATG 5 cut(s) 648, 724, 818, 1189, 1261
BseJI GATNNNNATC 1 cut(s) 1116
BseLI CCNNNNNNNGG 1 cut(s) 645
BseMI GCAATG 1 cut(s) 748
BseMII CTCAG 4 cut(s) 335, 617, 1118, 1202
BseRI GAGGAG 2 cut(s) 317, 413
BseXI GCAGC 2 cut(s) 241, 1219
BsgI GTGCAG 2 cut(s) 248, 752
BshFI GGCC 5 cut(s) 30, 254, 319, 662, 1132
BshNI GGYRCC 1 cut(s) 956
BshTI ACCGGT 1 cut(s) 1155
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 4 cut(s) 468, 640, 716, 1156
BsiWI CGTACG 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 645
BsmAI GTCTC 2 cut(s) 705, 929
BsnI GGCC 5 cut(s) 30, 254, 319, 662, 1132
Bso31I GGTCTC 1 cut(s) 929
BsoBI CYCGRG 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 819
Bsp13I TCCGGA 1 cut(s) 715
Bsp143I GATC 4 cut(s) 166, 628, 879, 1117
Bsp19I CCATGG 1 cut(s) 666
BspACI CCGC 5 cut(s) 224, 330, 646, 1056, 1137
BspANI GGCC 5 cut(s) 30, 254, 319, 662, 1132
BspCNI CTCAG 4 cut(s) 336, 616, 1119, 1203
BspEI TCCGGA 1 cut(s) 715
BspLI GGNNCC 3 cut(s) 402, 958, 994
BspMAI CTGCAG 2 cut(s) 79, 1209
BspMI ACCTGC 1 cut(s) 1092
BspPI GGATC 3 cut(s) 623, 887, 1125
BspT104I TTCGAA 1 cut(s) 819
BspT107I GGYRCC 1 cut(s) 956
BspTNI GGTCTC 1 cut(s) 929
BsrBI CCGCTC 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 748
BsrFI RCCGGY 1 cut(s) 1155
BssAI RCCGGY 1 cut(s) 1155
BssECI CCNNGG 3 cut(s) 638, 666, 905
BssMI GATC 4 cut(s) 166, 628, 879, 1117
BssNI GRCGYC 2 cut(s) 123, 957
BssT1I CCWWGG 1 cut(s) 666
Bst2UI CCWGG 2 cut(s) 906, 1134
Bst4CI ACNGT 3 cut(s) 623, 868, 1023
Bst6I CTCTTC 2 cut(s) 423, 543
BstACI GRCGYC 2 cut(s) 123, 957
BstBI TTCGAA 1 cut(s) 819
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 5 cut(s) 344, 598, 603, 1127, 1211
BstDSI CCRYGG 1 cut(s) 666
BstEII GGTNACC 2 cut(s) 357, 847
BstF5I GGATG 5 cut(s) 648, 724, 818, 1189, 1261
BstH2I RGCGCY 1 cut(s) 960
BstHHI GCGC 1 cut(s) 959
BstKTI GATC 4 cut(s) 169, 631, 882, 1120
BstMAI GTCTC 2 cut(s) 705, 929
BstMBI GATC 4 cut(s) 166, 628, 879, 1117
BstMWI GCNNNNNNNGC 2 cut(s) 16, 643
BstNI CCWGG 2 cut(s) 906, 1134
BstNSI RCATGY 2 cut(s) 521, 1201
BstPI GGTNACC 2 cut(s) 357, 847
BstSCI CCNGG 4 cut(s) 467, 638, 904, 1132
BstSFI CTRYAG 3 cut(s) 75, 867, 1205
BstV1I GCAGC 2 cut(s) 241, 1219
BstV2I GAAGAC 1 cut(s) 105
BsuRI GGCC 5 cut(s) 30, 254, 319, 662, 1132
BtgI CCRYGG 1 cut(s) 666
BtsCI GGATG 5 cut(s) 648, 724, 818, 1189, 1261
BtsI GCAGTG 1 cut(s) 740
BtsIMutI CAGTG 2 cut(s) 740, 889
BveI ACCTGC 1 cut(s) 1092
Cac8I GCNNGC 1 cut(s) 79
CaiI CAGNNNCTG 1 cut(s) 610
CfoI GCGC 1 cut(s) 959
Cfr10I RCCGGY 1 cut(s) 1155
Cfr13I GGNCC 1 cut(s) 660
CseI GACGC 1 cut(s) 112
Csp6I GTAC 3 cut(s) 136, 286, 553
CspAI ACCGGT 1 cut(s) 1155
CviAII CATG 6 cut(s) 518, 556, 667, 1045, 1192, 1198
CviQI GTAC 3 cut(s) 136, 286, 553
DdeI CTNAG 5 cut(s) 344, 598, 603, 1127, 1211
DinI GGCGCC 1 cut(s) 958
DpnI GATC 4 cut(s) 168, 630, 881, 1119
DpnII GATC 4 cut(s) 166, 628, 879, 1117
DriI GACNNNNNGTC 1 cut(s) 571
Eam1104I CTCTTC 2 cut(s) 423, 543
Eam1105I GACNNNNNGTC 1 cut(s) 571
EarI CTCTTC 2 cut(s) 423, 543
EciI GGCGGA 1 cut(s) 1152
Eco130I CCWWGG 1 cut(s) 666
Eco147I AGGCCT 4 cut(s) 30, 254, 319, 1132
Eco31I GGTCTC 1 cut(s) 929
Eco57I CTGAAG 1 cut(s) 390
Eco88I CYCGRG 1 cut(s) 215
Eco91I GGTNACC 2 cut(s) 357, 847
EcoO109I RGGNCCY 1 cut(s) 660
EcoO65I GGTNACC 2 cut(s) 357, 847
EcoRI GAATTC 1 cut(s) 1122
EcoRII CCWGG 2 cut(s) 904, 1132
EcoT14I CCWWGG 1 cut(s) 666
EgeI GGCGCC 1 cut(s) 958
EheI GGCGCC 1 cut(s) 958
ErhI CCWWGG 1 cut(s) 666
FaeI CATG 6 cut(s) 521, 559, 670, 1048, 1195, 1201
FalI AAGNNNNNCTT 2 cut(s) 1251, 1283
FatI CATG 6 cut(s) 517, 555, 666, 1044, 1191, 1197
FauNDI CATATG 2 cut(s) 50, 104
FblI GTMKAC 2 cut(s) 672, 1263
Fnu4HI GCNGC 2 cut(s) 230, 1208
FokI GGATG 5 cut(s) 655, 731, 825, 1196, 1268
Fsp4HI GCNGC 2 cut(s) 230, 1208
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 958
GluI GCNGC 2 cut(s) 230, 1208
GsuI CTGGAG 1 cut(s) 320
HaeII RGCGCY 1 cut(s) 960
HaeIII GGCC 5 cut(s) 30, 254, 319, 662, 1132
HapII CCGG 4 cut(s) 468, 640, 716, 1156
HgaI GACGC 1 cut(s) 112
HhaI GCGC 1 cut(s) 959
Hin1I GRCGYC 2 cut(s) 123, 957
Hin1II CATG 6 cut(s) 521, 559, 670, 1048, 1195, 1201
Hin6I GCGC 1 cut(s) 957
HinP1I GCGC 1 cut(s) 957
HindIII AAGCTT 4 cut(s) 156, 199, 473, 836
HinfI GANTC 5 cut(s) 218, 352, 698, 1013, 1048
HpaII CCGG 4 cut(s) 468, 640, 716, 1156
Hpy166II GTNNAC 2 cut(s) 673, 1264
Hpy188I TCNGA 8 cut(s) 15, 166, 345, 515, 606, 812, 879, 1128
Hpy188III TCNNGA 4 cut(s) 299, 716, 926, 1067
Hpy8I GTNNAC 2 cut(s) 673, 1264
HpyAV CCTTC 5 cut(s) 98, 134, 235, 365, 823
HpyCH4III ACNGT 3 cut(s) 623, 868, 1023
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 643
HpyF3I CTNAG 5 cut(s) 344, 598, 603, 1127, 1211
Hsp92I GRCGYC 2 cut(s) 123, 957
Hsp92II CATG 6 cut(s) 521, 559, 670, 1048, 1195, 1201
HspAI GCGC 1 cut(s) 957
KasI GGCGCC 1 cut(s) 956
Kpn2I TCCGGA 1 cut(s) 715
Kzo9I GATC 4 cut(s) 166, 628, 879, 1117
LmnI GCTCC 2 cut(s) 400, 738
Lsp1109I GCAGC 2 cut(s) 241, 1219
LweI GCATC 4 cut(s) 6, 121, 603, 633
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 2 cut(s) 357, 847
MalI GATC 4 cut(s) 168, 630, 881, 1119
MbiI CCGCTC 1 cut(s) 332
MboI GATC 4 cut(s) 166, 628, 879, 1117
MboII GAAGA 4 cut(s) 110, 440, 560, 1153
MluCI AATT 6 cut(s) 39, 113, 234, 278, 742, 1122
Mly113I GGCGCC 1 cut(s) 957
MlyI GAGTC 2 cut(s) 227, 707
MmeI TCCRAC 3 cut(s) 525, 790, 1260
MroI TCCGGA 1 cut(s) 715
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 7 cut(s) 117, 411, 477, 618, 762, 840, 900
MslI CAYNNNNRTG 3 cut(s) 419, 522, 1196
MspA1I CMGCKG 3 cut(s) 224, 1058, 1210
MspI CCGG 4 cut(s) 468, 640, 716, 1156
MspR9I CCNGG 4 cut(s) 469, 640, 906, 1134
MvaI CCWGG 2 cut(s) 906, 1134
MwoI GCNNNNNNNGC 2 cut(s) 16, 643
NarI GGCGCC 1 cut(s) 957
NciI CCSGG 2 cut(s) 469, 640
NcoI CCATGG 1 cut(s) 666
NdeI CATATG 2 cut(s) 50, 104
NdeII GATC 4 cut(s) 166, 628, 879, 1117
NlaIII CATG 6 cut(s) 521, 559, 670, 1048, 1195, 1201
NlaIV GGNNCC 3 cut(s) 402, 958, 994
NspI RCATGY 2 cut(s) 521, 1201
NspV TTCGAA 1 cut(s) 819
OliI CACNNNNGTG 1 cut(s) 419
PaeR7I CTCGAG 1 cut(s) 215
PceI AGGCCT 4 cut(s) 30, 254, 319, 1132
PcsI WCGNNNNNNNCGW 1 cut(s) 816
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 3 cut(s) 352, 1013, 1048
Pfl23II CGTACG 1 cut(s) 135
PinAI ACCGGT 1 cut(s) 1155
PkrI GCNGC 2 cut(s) 231, 1209
PleI GAGTC 2 cut(s) 226, 706
PluTI GGCGCC 1 cut(s) 960
PpsI GAGTC 2 cut(s) 226, 706
PsiI TTATAA 2 cut(s) 38, 1005
Psp6I CCWGG 2 cut(s) 904, 1132
PspEI GGTNACC 2 cut(s) 357, 847
PspGI CCWGG 2 cut(s) 904, 1132
PspLI CGTACG 1 cut(s) 135
PspN4I GGNNCC 3 cut(s) 402, 958, 994
PspPI GGNCC 1 cut(s) 660
PspXI VCTCGAGB 1 cut(s) 215
PsrI GAACNNNNNNTAC 2 cut(s) 165, 197
PstI CTGCAG 2 cut(s) 79, 1209
PstNI CAGNNNCTG 1 cut(s) 610
PvuII CAGCTG 1 cut(s) 1210
RsaI GTAC 3 cut(s) 137, 287, 554
RsaNI GTAC 3 cut(s) 136, 286, 553
RseI CAYNNNNRTG 3 cut(s) 419, 522, 1196
SaqAI TTAA 7 cut(s) 117, 411, 477, 618, 762, 840, 900
SatI GCNGC 2 cut(s) 230, 1208
Sau3AI GATC 4 cut(s) 166, 628, 879, 1117
Sau96I GGNCC 1 cut(s) 660
SchI GAGTC 2 cut(s) 227, 707
ScrFI CCNGG 4 cut(s) 469, 640, 906, 1134
SfaNI GCATC 4 cut(s) 6, 121, 603, 633
SfcI CTRYAG 3 cut(s) 75, 867, 1205
SfoI GGCGCC 1 cut(s) 958
Sfr274I CTCGAG 1 cut(s) 215
SfuI TTCGAA 1 cut(s) 819
SlaI CTCGAG 1 cut(s) 215
SmiMI CAYNNNNRTG 3 cut(s) 419, 522, 1196
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 6 cut(s) 39, 113, 234, 278, 742, 1122
SseBI AGGCCT 4 cut(s) 30, 254, 319, 1132
SsiI CCGC 5 cut(s) 224, 330, 646, 1056, 1137
SspDI GGCGCC 1 cut(s) 956
SspMI CTAG 1 cut(s) 194
StuI AGGCCT 4 cut(s) 30, 254, 319, 1132
StyD4I CCNGG 4 cut(s) 467, 638, 904, 1132
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 3 cut(s) 623, 868, 1023
TaqI TCGA 5 cut(s) 169, 216, 819, 927, 1120
TasI AATT 6 cut(s) 39, 113, 234, 278, 742, 1122
TatI WGTACW 2 cut(s) 285, 552
TfiI GAWTC 3 cut(s) 352, 1013, 1048
Tru1I TTAA 7 cut(s) 117, 411, 477, 618, 762, 840, 900
Tru9I TTAA 7 cut(s) 117, 411, 477, 618, 762, 840, 900
TscAI CASTG 2 cut(s) 740, 889
TseI GCWGC 2 cut(s) 229, 1207
TspDTI ATGAA 4 cut(s) 111, 407, 1180, 1272
TspRI CASTG 2 cut(s) 740, 889
XapI RAATTY 2 cut(s) 278, 1122
XceI RCATGY 2 cut(s) 521, 1201
XhoI CTCGAG 1 cut(s) 215
XmiI GTMKAC 2 cut(s) 672, 1263
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.