pycom04g17960

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012143
Physical Location & Seq
Forward (+)
20135785 .. 20137194
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g17960.1

Sequence Viewer

Length: 1410 bp
ATGGCGAGGTTGTGTCATCCGAAATGTAGACTAGGTAATAAATCAATGGGTTGGCTTCAGATTCTACTAGGAGGCCTTGTTATAATTTTGAGCATATGCAGTCTCGTGAAGTTCTACTCTGCTGGGTTTTTCTTCCACAATGAAGATATATGCCGACAATTTACTGGCGTCAAAGATGCATACGAAGGGTTTGACATAAAAGCTTTGTCTGATCGAGTAGGGGAAGTTCTAAATAAATTGGAAAGCTTACAAGAAAAGCTCGAGTCAGCTGTCCAGCAAATGGAGAAGGACAAAGAGACTTTACGTACAACTAACATTACAAAATTCGAGTACAAGAAGTATCTGGAGGAGGACGTAATCAGGCCTCTTTATGCCGCTCACATTTCTCTGAGGCTGATTCGGTTACCTAAAGCCGAAGGTATTCACAACTCAACGATGAAAGAGGAGCCTTTGATTAACACTTTTGTGATTGAAGAGATAAGGAAGTATATAACCCCAAAGGAGAGTAGAACCGGGAAGCTTAATATATATGGAACAGAAAGGATATACAATACAATTGGACATGCTTGTGTGTTATACAAGAAAGAGTTGGAAGAGTACATGAGCTATGATATCGGGTCTTATTGTAACGATGATTGGAACTTAGCTCAGAAGCTGATGCTTAACGGTTGTGATCCTCTGCCTCGGAGGCGGTGCTTGACAAGGGCCTCAATGGTCTACCAGAAGCCTTACCCTATCAATGAGTCTCTTTGGAAACTGCCGGATGATAGAAATGTGCGGTGGAGCAATTATCAGTGCAGGAACTTTAAGTGCTTGATGGGAAACAACACCAAGAAGGGTTATTTCAAGTGTGTCGGGTGTTTCGAAATGGAGAAGGAAAAGCTTAAATGGGTTACAAATAGCTCACTTTCCGTTGATTTTCTGATCAGTGATGTTTTGACTATTAAGCCCGGGGAGATGAGGATTGGTCTCGACTTTGGCATTGGCACTGGAAGTTTCGCTGCAAGGATGAGAGAACATAACATTACAGTAGTCTCTACTGCTCTTAACCTCAAGGCGCCTTTCAACGAGATGATTGCACTGAGAGGTTTGATTCCATTGTTTGTAACATTGAATCAACGCCTACCATTCTTCGACAACACAATGGACCTGATTCACACCAGTGGATTTCTAGACGGCTGGATTGACCTGCAACTGCTGGATTTTATCCTGTTTGATTGGGATCGAATTCTGAGGCCAGGCGGATTGTTATGGATTGACCGGTTCTTCTGTGTTAGAAAGGATTTGGATGATTTCATGTACATGTTTCTGCAGCTGAGGTACAAGAAACACAAGTGGGCTATTTCTCCTAAATCAAAGGATGAGGTCTACTTTTCTGCGCTGTTGGAGAAACCTCCAAGAGCAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

470

Amino Acids

54.66

Weight (kDa)

8.84

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 305 - 417 3.8e-08 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05390
fragaria_vesca FvH4_6g06250 FvH4_6g06250
malus_domestica MD12G1217000.v1.1
prunus_persica Prupe.6G323300_v2.0.a1
pyrus_communis pycom04g17960
rosa_chinensis RchiOBHm_Chr3g0455271
rosa_laevigata RLG00000025373
rosa_multiflora Rmu_sc0002169.1_g000037
rosa_roxburghii Rroxscaffold_6G00424410
rosa_rugosa Rorug03G0005500 Rorug03G0005600 Rorug03G0005700 Rorug03G0005800
rosa_samantha Rh3AG065600 Rh3BG067800 Rh3CG066200 Rh3DG067800
rosa_wichuraiana Rw3G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 83
Acc36I ACCTGC 1 cut(s) 1197
AccB1I GGYRCC 1 cut(s) 1057
AccB7I CCANNNNNTGG 1 cut(s) 280
AccBSI CCGCTC 1 cut(s) 377
AccI GTMKAC 3 cut(s) 28, 717, 1368
AciI CCGC 4 cut(s) 375, 691, 778, 1242
AclWI GGATC 2 cut(s) 668, 1230
AcsI RAATTY 2 cut(s) 323, 1227
AcuI CTGAAG 1 cut(s) 41
AcyI GRCGYC 2 cut(s) 168, 1058
AfaI GTAC 5 cut(s) 307, 332, 599, 1301, 1322
AfiI CCNNNNNNNGG 1 cut(s) 280
AflIII ACRYGT 1 cut(s) 1302
AgeI ACCGGT 1 cut(s) 1260
AgsI TTSAA 4 cut(s) 473, 847, 1066, 1114
AjnI CCWGG 1 cut(s) 1237
AleI CACNNNNGTG 2 cut(s) 464, 1161
Alw26I GTCTC 5 cut(s) 107, 290, 750, 974, 1039
AlwI GGATC 2 cut(s) 668, 1230
AlwNI CAGNNNCTG 1 cut(s) 655
Ama87I CYCGRG 2 cut(s) 260, 950
AoxI GGCC 4 cut(s) 73, 362, 705, 1235
ApeKI GCWGC 2 cut(s) 1001, 1312
ApoI RAATTY 2 cut(s) 323, 1227
AsiGI ACCGGT 1 cut(s) 1260
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 2 cut(s) 1060, 1381
AspS9I GGNCC 2 cut(s) 705, 1147
AsuC2I CCSGG 3 cut(s) 514, 951, 952
AsuII TTCGAA 1 cut(s) 864
AvaI CYCGRG 2 cut(s) 260, 950
AvaII GGWCC 1 cut(s) 1147
BanI GGYRCC 1 cut(s) 1057
BauI CACGAG 1 cut(s) 104
BbvCI CCTCAGC 1 cut(s) 1316
BbvI GCAGC 2 cut(s) 988, 1324
BccI CCATC 1 cut(s) 811
BceAI ACGGC 1 cut(s) 1192
BcgI CGANNNNNNTGC 2 cut(s) 1058, 1092
BciT130I CCWGG 1 cut(s) 1239
BclI TGATCA 1 cut(s) 924
BcnI CCSGG 3 cut(s) 514, 951, 952
BcoDI GTCTC 5 cut(s) 107, 290, 750, 974, 1039
BfaI CTAG 3 cut(s) 32, 68, 1172
BfmI CTRYAG 1 cut(s) 1310
BfoI RGCGCY 1 cut(s) 1061
BfuAI ACCTGC 1 cut(s) 1197
BglI GCCNNNNNGGC 1 cut(s) 688
BisI GCNGC 3 cut(s) 375, 1002, 1313
BlsI GCNGC 3 cut(s) 376, 1003, 1314
Bme1390I CCNGG 4 cut(s) 514, 951, 952, 1239
Bme18I GGWCC 1 cut(s) 1147
BmeT110I CYCGRG 2 cut(s) 260, 950
BmgT120I GGNCC 2 cut(s) 705, 1147
BmiI GGNNCC 2 cut(s) 447, 1059
BmrFI CCNGG 4 cut(s) 514, 951, 952, 1239
BmsI GCATC 2 cut(s) 166, 648
BpmI CTGGAG 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 1316
Bpu14I TTCGAA 1 cut(s) 864
BpuEI CTTGAG 1 cut(s) 1037
BpuMI CCSGG 3 cut(s) 514, 951, 952
BsaAI YACGTR 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 1221
BsaHI GRCGYC 2 cut(s) 168, 1058
BsaI GGTCTC 1 cut(s) 974
BsaJI CCNNGG 3 cut(s) 683, 950, 951
BsaWI WCCGGW 1 cut(s) 1260
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse118I RCCGGY 1 cut(s) 1260
Bse1I ACTGG 3 cut(s) 169, 994, 1161
Bse8I GATNNNNATC 1 cut(s) 1221
BseBI CCWGG 1 cut(s) 1239
BseDI CCNNGG 3 cut(s) 683, 950, 951
BseGI GGATG 5 cut(s) 16, 769, 1014, 1294, 1366
BseJI GATNNNNATC 1 cut(s) 1221
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMII CTCAG 5 cut(s) 380, 662, 1073, 1223, 1307
BseNI ACTGG 3 cut(s) 169, 994, 1161
BseRI GAGGAG 2 cut(s) 362, 458
BseXI GCAGC 2 cut(s) 988, 1324
BseYI CCCAGC 1 cut(s) 122
BsgI GTGCAG 1 cut(s) 817
BshFI GGCC 4 cut(s) 75, 364, 707, 1237
BshNI GGYRCC 1 cut(s) 1057
BshTI ACCGGT 1 cut(s) 1260
BsiHKCI CYCGRG 2 cut(s) 260, 950
BsiSI CCGG 4 cut(s) 513, 761, 951, 1261
BslI CCNNNNNNNGG 1 cut(s) 280
BsmAI GTCTC 5 cut(s) 107, 290, 750, 974, 1039
BsnI GGCC 4 cut(s) 75, 364, 707, 1237
Bso31I GGTCTC 1 cut(s) 974
BsoBI CYCGRG 2 cut(s) 260, 950
Bsp119I TTCGAA 1 cut(s) 864
Bsp1407I TGTACA 1 cut(s) 1299
Bsp143I GATC 4 cut(s) 211, 673, 924, 1222
BspACI CCGC 4 cut(s) 375, 691, 778, 1242
BspANI GGCC 4 cut(s) 75, 364, 707, 1237
BspCNI CTCAG 5 cut(s) 381, 661, 1074, 1224, 1308
BspLI GGNNCC 2 cut(s) 447, 1059
BspMAI CTGCAG 1 cut(s) 1314
BspMI ACCTGC 1 cut(s) 1197
BspPI GGATC 2 cut(s) 668, 1230
BspT104I TTCGAA 1 cut(s) 864
BspT107I GGYRCC 1 cut(s) 1057
BspTNI GGTCTC 1 cut(s) 974
BsrBI CCGCTC 1 cut(s) 377
BsrFI RCCGGY 1 cut(s) 1260
BsrGI TGTACA 1 cut(s) 1299
BsrI ACTGG 3 cut(s) 169, 994, 1161
BssAI RCCGGY 1 cut(s) 1260
BssECI CCNNGG 3 cut(s) 683, 950, 951
BssMI GATC 4 cut(s) 211, 673, 924, 1222
BssNI GRCGYC 2 cut(s) 168, 1058
BssSI CACGAG 1 cut(s) 104
Bst2BI CACGAG 1 cut(s) 104
Bst2UI CCWGG 1 cut(s) 1239
Bst4CI ACNGT 2 cut(s) 668, 1030
Bst6I CTCTTC 2 cut(s) 468, 588
BstACI GRCGYC 2 cut(s) 168, 1058
BstAUI TGTACA 1 cut(s) 1299
BstBAI YACGTR 1 cut(s) 305
BstBI TTCGAA 1 cut(s) 864
BstDEI CTNAG 6 cut(s) 389, 643, 648, 1082, 1232, 1316
BstEII GGTNACC 1 cut(s) 402
BstF5I GGATG 5 cut(s) 16, 769, 1014, 1294, 1366
BstH2I RGCGCY 1 cut(s) 1061
BstHHI GCGC 2 cut(s) 1060, 1381
BstKTI GATC 4 cut(s) 214, 676, 927, 1225
BstMAI GTCTC 5 cut(s) 107, 290, 750, 974, 1039
BstMBI GATC 4 cut(s) 211, 673, 924, 1222
BstMWI GCNNNNNNNGC 1 cut(s) 688
BstNI CCWGG 1 cut(s) 1239
BstNSI RCATGY 2 cut(s) 566, 1306
BstPI GGTNACC 1 cut(s) 402
BstSCI CCNGG 4 cut(s) 512, 949, 950, 1237
BstSFI CTRYAG 1 cut(s) 1310
BstSNI TACGTA 1 cut(s) 305
BstV1I GCAGC 2 cut(s) 988, 1324
BsuRI GGCC 4 cut(s) 75, 364, 707, 1237
BtsCI GGATG 5 cut(s) 16, 769, 1014, 1294, 1366
BtsIMutI CAGTG 5 cut(s) 800, 934, 987, 1079, 1168
BveI ACCTGC 1 cut(s) 1197
CaiI CAGNNNCTG 1 cut(s) 655
CfoI GCGC 2 cut(s) 1060, 1381
Cfr10I RCCGGY 1 cut(s) 1260
Cfr13I GGNCC 2 cut(s) 705, 1147
Cfr9I CCCGGG 1 cut(s) 950
CseI GACGC 1 cut(s) 157
Csp6I GTAC 5 cut(s) 306, 331, 598, 1300, 1321
CspAI ACCGGT 1 cut(s) 1260
CviAII CATG 4 cut(s) 563, 601, 1297, 1303
CviQI GTAC 5 cut(s) 306, 331, 598, 1300, 1321
DdeI CTNAG 6 cut(s) 389, 643, 648, 1082, 1232, 1316
DinI GGCGCC 1 cut(s) 1059
DpnI GATC 4 cut(s) 213, 675, 926, 1224
DpnII GATC 4 cut(s) 211, 673, 924, 1222
Eam1104I CTCTTC 2 cut(s) 468, 588
EarI CTCTTC 2 cut(s) 468, 588
EciI GGCGGA 1 cut(s) 1257
Eco105I TACGTA 1 cut(s) 305
Eco147I AGGCCT 2 cut(s) 75, 364
Eco31I GGTCTC 1 cut(s) 974
Eco32I GATATC 1 cut(s) 613
Eco47I GGWCC 1 cut(s) 1147
Eco57I CTGAAG 1 cut(s) 41
Eco88I CYCGRG 2 cut(s) 260, 950
Eco91I GGTNACC 1 cut(s) 402
EcoO109I RGGNCCY 1 cut(s) 705
EcoO65I GGTNACC 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 1227
EcoRII CCWGG 1 cut(s) 1237
EcoRV GATATC 1 cut(s) 613
EcoT22I ATGCAT 1 cut(s) 181
EgeI GGCGCC 1 cut(s) 1059
EheI GGCGCC 1 cut(s) 1059
FaeI CATG 4 cut(s) 566, 604, 1300, 1306
FatI CATG 4 cut(s) 562, 600, 1296, 1302
FauNDI CATATG 1 cut(s) 95
FbaI TGATCA 1 cut(s) 924
FblI GTMKAC 3 cut(s) 28, 717, 1368
Fnu4HI GCNGC 3 cut(s) 375, 1002, 1313
FokI GGATG 5 cut(s) 3, 776, 1021, 1301, 1373
Fsp4HI GCNGC 3 cut(s) 375, 1002, 1313
FspBI CTAG 3 cut(s) 32, 68, 1172
GlaI GCGC 2 cut(s) 1059, 1380
GluI GCNGC 3 cut(s) 375, 1002, 1313
GsaI CCCAGC 1 cut(s) 126
GsuI CTGGAG 1 cut(s) 365
HaeII RGCGCY 1 cut(s) 1061
HaeIII GGCC 4 cut(s) 75, 364, 707, 1237
HapII CCGG 4 cut(s) 513, 761, 951, 1261
HgaI GACGC 1 cut(s) 157
HhaI GCGC 2 cut(s) 1060, 1381
Hin1I GRCGYC 2 cut(s) 168, 1058
Hin1II CATG 4 cut(s) 566, 604, 1300, 1306
Hin6I GCGC 2 cut(s) 1058, 1379
HinP1I GCGC 2 cut(s) 1058, 1379
HindIII AAGCTT 4 cut(s) 201, 244, 518, 881
HinfI GANTC 7 cut(s) 61, 263, 397, 743, 1093, 1114, 1153
HpaII CCGG 4 cut(s) 513, 761, 951, 1261
Hpy166II GTNNAC 3 cut(s) 29, 718, 1369
Hpy188I TCNGA 8 cut(s) 21, 60, 211, 390, 651, 687, 924, 1233
Hpy188III TCNNGA 4 cut(s) 106, 344, 971, 1172
Hpy8I GTNNAC 3 cut(s) 29, 718, 1369
HpyAV CCTTC 5 cut(s) 179, 280, 410, 829, 868
HpyCH4III ACNGT 2 cut(s) 668, 1030
HpyCH4IV ACGT 2 cut(s) 304, 354
HpyCH4V TGCA 7 cut(s) 99, 179, 798, 1004, 1079, 1192, 1312
HpyF10VI GCNNNNNNNGC 1 cut(s) 688
HpyF3I CTNAG 6 cut(s) 389, 643, 648, 1082, 1232, 1316
HpySE526I ACGT 2 cut(s) 304, 354
Hsp92I GRCGYC 2 cut(s) 168, 1058
Hsp92II CATG 4 cut(s) 566, 604, 1300, 1306
HspAI GCGC 2 cut(s) 1058, 1379
KasI GGCGCC 1 cut(s) 1057
Ksp22I TGATCA 1 cut(s) 924
Kzo9I GATC 4 cut(s) 211, 673, 924, 1222
LmnI GCTCC 2 cut(s) 445, 783
Lsp1109I GCAGC 2 cut(s) 988, 1324
LweI GCATC 2 cut(s) 166, 648
MaeI CTAG 3 cut(s) 32, 68, 1172
MaeII ACGT 2 cut(s) 304, 354
MaeIII GTNAC 4 cut(s) 402, 626, 892, 1105
MalI GATC 4 cut(s) 213, 675, 926, 1224
MbiI CCGCTC 1 cut(s) 377
MboI GATC 4 cut(s) 211, 673, 924, 1222
MboII GAAGA 6 cut(s) 124, 155, 485, 605, 1123, 1258
MfeI CAATTG 1 cut(s) 555
MluCI AATT 7 cut(s) 84, 158, 236, 323, 555, 787, 1227
Mly113I GGCGCC 1 cut(s) 1058
MlyI GAGTC 2 cut(s) 272, 752
MmeI TCCRAC 2 cut(s) 570, 1365
Mph1103I ATGCAT 1 cut(s) 181
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 7 cut(s) 456, 522, 663, 807, 885, 945, 1047
MslI CAYNNNNRTG 4 cut(s) 464, 567, 1161, 1301
MspA1I CMGCKG 2 cut(s) 269, 1315
MspI CCGG 4 cut(s) 513, 761, 951, 1261
MspR9I CCNGG 4 cut(s) 514, 951, 952, 1239
MunI CAATTG 1 cut(s) 555
MvaI CCWGG 1 cut(s) 1239
MwoI GCNNNNNNNGC 1 cut(s) 688
NarI GGCGCC 1 cut(s) 1058
NciI CCSGG 3 cut(s) 514, 951, 952
NdeI CATATG 1 cut(s) 95
NdeII GATC 4 cut(s) 211, 673, 924, 1222
NlaIII CATG 4 cut(s) 566, 604, 1300, 1306
NlaIV GGNNCC 2 cut(s) 447, 1059
NsiI ATGCAT 1 cut(s) 181
NspI RCATGY 2 cut(s) 566, 1306
NspV TTCGAA 1 cut(s) 864
OliI CACNNNNGTG 2 cut(s) 464, 1161
PaeR7I CTCGAG 1 cut(s) 260
PceI AGGCCT 2 cut(s) 75, 364
PciI ACATGT 1 cut(s) 1302
PcsI WCGNNNNNNNCGW 1 cut(s) 861
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 5 cut(s) 61, 397, 1093, 1114, 1153
PflMI CCANNNNNTGG 1 cut(s) 280
PinAI ACCGGT 1 cut(s) 1260
PkrI GCNGC 3 cut(s) 376, 1003, 1314
PleI GAGTC 2 cut(s) 271, 751
PluTI GGCGCC 1 cut(s) 1061
PpsI GAGTC 2 cut(s) 271, 751
Ppu21I YACGTR 1 cut(s) 305
PscI ACATGT 1 cut(s) 1302
PsiI TTATAA 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 1237
PspEI GGTNACC 1 cut(s) 402
PspFI CCCAGC 1 cut(s) 122
PspGI CCWGG 1 cut(s) 1237
PspN4I GGNNCC 2 cut(s) 447, 1059
PspPI GGNCC 2 cut(s) 705, 1147
PspXI VCTCGAGB 1 cut(s) 260
PsrI GAACNNNNNNTAC 2 cut(s) 210, 242
PstI CTGCAG 1 cut(s) 1314
PstNI CAGNNNCTG 1 cut(s) 655
PvuII CAGCTG 2 cut(s) 269, 1315
RsaI GTAC 5 cut(s) 307, 332, 599, 1301, 1322
RsaNI GTAC 5 cut(s) 306, 331, 598, 1300, 1321
RseI CAYNNNNRTG 4 cut(s) 464, 567, 1161, 1301
SaqAI TTAA 7 cut(s) 456, 522, 663, 807, 885, 945, 1047
SatI GCNGC 3 cut(s) 375, 1002, 1313
Sau3AI GATC 4 cut(s) 211, 673, 924, 1222
Sau96I GGNCC 2 cut(s) 705, 1147
SchI GAGTC 2 cut(s) 272, 752
ScrFI CCNGG 4 cut(s) 514, 951, 952, 1239
SfaNI GCATC 2 cut(s) 166, 648
SfcI CTRYAG 1 cut(s) 1310
SfoI GGCGCC 1 cut(s) 1059
Sfr274I CTCGAG 1 cut(s) 260
SfuI TTCGAA 1 cut(s) 864
SinI GGWCC 1 cut(s) 1147
SlaI CTCGAG 1 cut(s) 260
SmaI CCCGGG 1 cut(s) 952
SmiMI CAYNNNNRTG 4 cut(s) 464, 567, 1161, 1301
SmlI CTYRAG 2 cut(s) 260, 1052
SmoI CTYRAG 2 cut(s) 260, 1052
SnaBI TACGTA 1 cut(s) 305
Sse9I AATT 7 cut(s) 84, 158, 236, 323, 555, 787, 1227
SseBI AGGCCT 2 cut(s) 75, 364
SsiI CCGC 4 cut(s) 375, 691, 778, 1242
SspDI GGCGCC 1 cut(s) 1057
SspMI CTAG 3 cut(s) 32, 68, 1172
StuI AGGCCT 2 cut(s) 75, 364
StyD4I CCNGG 4 cut(s) 512, 949, 950, 1237
TaaI ACNGT 2 cut(s) 668, 1030
TaiI ACGT 2 cut(s) 307, 357
TaqI TCGA 7 cut(s) 214, 261, 327, 864, 972, 1134, 1225
TasI AATT 7 cut(s) 84, 158, 236, 323, 555, 787, 1227
TatI WGTACW 3 cut(s) 330, 597, 1299
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 5 cut(s) 61, 397, 1093, 1114, 1153
Tru1I TTAA 7 cut(s) 456, 522, 663, 807, 885, 945, 1047
Tru9I TTAA 7 cut(s) 456, 522, 663, 807, 885, 945, 1047
TscAI CASTG 5 cut(s) 800, 934, 994, 1086, 1168
TseI GCWGC 2 cut(s) 1001, 1312
TspDTI ATGAA 3 cut(s) 156, 452, 1285
TspGWI ACGGA 1 cut(s) 901
TspMI CCCGGG 1 cut(s) 950
TspRI CASTG 5 cut(s) 800, 934, 994, 1086, 1168
Van91I CCANNNNNTGG 1 cut(s) 280
VpaK11BI GGWCC 1 cut(s) 1147
XapI RAATTY 2 cut(s) 323, 1227
XbaI TCTAGA 1 cut(s) 1171
XceI RCATGY 2 cut(s) 566, 1306
XhoI CTCGAG 1 cut(s) 260
XmaI CCCGGG 1 cut(s) 950
XmiI GTMKAC 3 cut(s) 28, 717, 1368
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 3 cut(s) 32, 68, 1172
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.