Prupe.6G323300_v2.0.a1

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0012143
Physical Location & Seq
Forward (+)
28498432 .. 28501557
3126 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G323300.1

Sequence Viewer

Length: 1404 bp
ATGGGGAGGTCATGCCATCCCAAATGTAGGCTGGGTAGATTAATGAGTTGGCTTCAGATTGTGTTGGGAGGCCTTGTTATAATTTTCAGCATATGTAGTCTCTTCAAGTTCTACTCTGCAGGGTTTTTCTTGCACAATGAGGACATATGCCGACAATTTTATGGCGTCAGGGAGGCATACGAAGGGTTCGACCTAAAAGCTTTGTCTGATCGAGTAGGGGAAGTGCTAAATAGACTGGAAAGCTTACAAGAAAAGCTTGAATCAGCTGTGCAGCAAATGGAGAAGAACAAAGTGGACTTGCATACAACTAATATTACAAAATTGGAGTACAAGAAGTATCTGGAGGAAGAAGTAATCAGGCCTCTTTATAGTGCTCACATTTCTCTGAGACTGATTCGGTTACCTAAGCCCGAAGGTATTAGAAACTCTACGATGAAGGAGGAGCCTTTGATTAACACTTTTGTGATTGAAGAGATAAGGAAGTATATTACACCAAAGCAGAGTAGAGTTGGAAAGATTACTATATATGGTACAGAGAGGATATCCAATACAATTGGACATGCCTGTGTGTTATACAAGAAAGAGCTGGAAGAGTACATGAGCTATGACATTGGGTCTTATTGTAAAGACGATTGGAACTTAGCACAGAAGCTGATGCTTAACGGTTGTGATCCTTTGCCCCGAAGGCGGTGCTTGACAAGGGCCTCCATGGTTTACCAGAAGCCTTACCCAATCAATGAGTCTCTGTGGAGATTGCCAGATGATAGAAATGTGAGGTGGAGCAAATATCTGTGCAGGAACTTTAAGTGCTTGATGGGCAATAACAGTAGGGGTTATTCTAAGTGTGTTGGGTGTTTTGAAATGGAGAAGGAAAAACTTAAATGGGTTACAAACAGCTCCCTTCCCATTGATTTTCTGATCAATGATGTGTTGGCTATTAAGCTGGGGGAGATAAGGATTGGTCTTGACTTTGGTATTGGTACAGGAACCTTTGCTGCAAGGATGAGGGAACATAATGTTACAATAGTCTCTACTGCTCTTAACCTTGGAGCTCCTTTCAATGAGATGATTGCACTGAGAGGTTTGATCCCATTGTACCTAACATTGAATCAACGGCTGCCATTCTTTGATAACATAATGGACATGATTCATACCAGTGGGTTTCTGGATGGCTGGATTGACCTGCAATTGCTGGATTTTATCCTGTTCGATTGGGATCGAATTCTGAGGCCGGGCGGGTTGTTATGGATTGACCGGTTCTTCTGTCAAAGAAAGGATTTGGACGTCTTCATGTACATGTTTCTGCAGCTGAGATACAAGAAGCACAAGTGGGCTATTTCTCCTAAATCAAAGGATGAGGTCTACTTTTCTGCACTGTTGGAGAAACCTCCAAGAGCAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

468

Amino Acids

54.57

Weight (kDa)

9.04

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05390
fragaria_vesca FvH4_6g06250 FvH4_6g06250
malus_domestica MD12G1217000.v1.1
prunus_persica Prupe.6G323300_v2.0.a1
pyrus_communis pycom04g17960
rosa_chinensis RchiOBHm_Chr3g0455271
rosa_laevigata RLG00000025373
rosa_multiflora Rmu_sc0002169.1_g000037
rosa_roxburghii Rroxscaffold_6G00424410
rosa_rugosa Rorug03G0005500 Rorug03G0005600 Rorug03G0005700 Rorug03G0005800
rosa_samantha Rh3AG065600 Rh3BG067800 Rh3CG066200 Rh3DG067800
rosa_wichuraiana Rw3G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 80
AatII GACGTC 1 cut(s) 1287
Acc36I ACCTGC 1 cut(s) 1191
AccI GTMKAC 1 cut(s) 1362
AciI CCGC 2 cut(s) 688, 1236
AclWI GGATC 3 cut(s) 665, 1081, 1224
AcsI RAATTY 1 cut(s) 1221
AcuI CTGAAG 1 cut(s) 38
AcyI GRCGYC 2 cut(s) 165, 1284
AfaI GTAC 6 cut(s) 329, 532, 596, 982, 1097, 1295
AfiI CCNNNNNNNGG 2 cut(s) 27, 687
AflIII ACRYGT 1 cut(s) 1296
AgeI ACCGGT 1 cut(s) 1254
AgsI TTSAA 6 cut(s) 106, 260, 470, 860, 1060, 1108
AhdI GACNNNNNGTC 1 cut(s) 613
AleI CACNNNNGTG 1 cut(s) 461
Alw21I GWGCWC 2 cut(s) 376, 1054
Alw26I GTCTC 4 cut(s) 104, 382, 747, 1033
AlwI GGATC 3 cut(s) 665, 1081, 1224
AlwNI CAGNNNCTG 1 cut(s) 652
AoxI GGCC 4 cut(s) 70, 359, 702, 1229
ApeKI GCWGC 4 cut(s) 271, 995, 1117, 1306
ApoI RAATTY 1 cut(s) 1221
AseI ATTAAT 1 cut(s) 41
AsiGI ACCGGT 1 cut(s) 1254
AspS9I GGNCC 1 cut(s) 702
AsuC2I CCSGG 1 cut(s) 1233
BanII GRGCYC 1 cut(s) 1054
BbsI GAAGAC 1 cut(s) 1279
Bbv12I GWGCWC 2 cut(s) 376, 1054
BbvI GCAGC 4 cut(s) 283, 982, 1104, 1318
BccI CCATC 3 cut(s) 24, 808, 1163
BceAI ACGGC 1 cut(s) 1130
BcgI CGANNNNNNTGC 2 cut(s) 672, 706
BclI TGATCA 1 cut(s) 918
BcnI CCSGG 1 cut(s) 1233
BcoDI GTCTC 4 cut(s) 104, 382, 747, 1033
BfmI CTRYAG 2 cut(s) 117, 1304
BfuAI ACCTGC 1 cut(s) 1191
BglI GCCNNNNNGGC 1 cut(s) 685
BisI GCNGC 4 cut(s) 272, 996, 1118, 1307
BlsI GCNGC 4 cut(s) 273, 997, 1119, 1308
Bme1390I CCNGG 1 cut(s) 1233
BmeRI GACNNNNNGTC 1 cut(s) 613
BmgT120I GGNCC 1 cut(s) 702
BmiI GGNNCC 2 cut(s) 444, 988
BmrFI CCNGG 1 cut(s) 1233
BmsI GCATC 1 cut(s) 645
BpiI GAAGAC 1 cut(s) 1279
BpmI CTGGAG 1 cut(s) 362
Bpu10I CCTNAGC 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 1233
BsaBI GATNNNNATC 1 cut(s) 1215
BsaHI GRCGYC 2 cut(s) 165, 1284
BsaJI CCNNGG 2 cut(s) 708, 1045
BsaWI WCCGGW 1 cut(s) 1254
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 2 cut(s) 27, 687
Bse118I RCCGGY 1 cut(s) 1254
Bse1I ACTGG 2 cut(s) 240, 1155
Bse8I GATNNNNATC 1 cut(s) 1215
BseDI CCNNGG 2 cut(s) 708, 1045
BseGI GGATG 4 cut(s) 16, 1008, 1174, 1360
BseJI GATNNNNATC 1 cut(s) 1215
BseLI CCNNNNNNNGG 2 cut(s) 27, 687
BseMII CTCAG 4 cut(s) 377, 1067, 1217, 1301
BseNI ACTGG 2 cut(s) 240, 1155
BseRI GAGGAG 1 cut(s) 455
BseXI GCAGC 4 cut(s) 283, 982, 1104, 1318
BseYI CCCAGC 2 cut(s) 31, 943
BsgI GTGCAG 3 cut(s) 290, 814, 1356
BshFI GGCC 4 cut(s) 72, 361, 704, 1231
BshTI ACCGGT 1 cut(s) 1254
BsiHKAI GWGCWC 2 cut(s) 376, 1054
BsiSI CCGG 2 cut(s) 1232, 1255
BslI CCNNNNNNNGG 2 cut(s) 27, 687
BsmAI GTCTC 4 cut(s) 104, 382, 747, 1033
BsnI GGCC 4 cut(s) 72, 361, 704, 1231
Bsp1286I GDGCHC 2 cut(s) 376, 1054
Bsp1407I TGTACA 1 cut(s) 1293
Bsp143I GATC 5 cut(s) 208, 670, 918, 1086, 1216
Bsp19I CCATGG 1 cut(s) 708
BspACI CCGC 2 cut(s) 688, 1236
BspANI GGCC 4 cut(s) 72, 361, 704, 1231
BspCNI CTCAG 4 cut(s) 378, 1068, 1218, 1302
BspLI GGNNCC 2 cut(s) 444, 988
BspMAI CTGCAG 2 cut(s) 121, 1308
BspMI ACCTGC 1 cut(s) 1191
BspPI GGATC 3 cut(s) 665, 1081, 1224
BsrFI RCCGGY 1 cut(s) 1254
BsrGI TGTACA 1 cut(s) 1293
BsrI ACTGG 2 cut(s) 240, 1155
BssAI RCCGGY 1 cut(s) 1254
BssECI CCNNGG 2 cut(s) 708, 1045
BssMI GATC 5 cut(s) 208, 670, 918, 1086, 1216
BssNI GRCGYC 2 cut(s) 165, 1284
BssT1I CCWWGG 2 cut(s) 708, 1045
Bst4CI ACNGT 3 cut(s) 665, 827, 1377
Bst6I CTCTTC 3 cut(s) 107, 465, 585
BstACI GRCGYC 2 cut(s) 165, 1284
BstAUI TGTACA 1 cut(s) 1293
BstDEI CTNAG 7 cut(s) 386, 405, 640, 840, 1076, 1226, 1310
BstDSI CCRYGG 1 cut(s) 708
BstEII GGTNACC 1 cut(s) 399
BstF5I GGATG 4 cut(s) 16, 1008, 1174, 1360
BstKTI GATC 5 cut(s) 211, 673, 921, 1089, 1219
BstMAI GTCTC 4 cut(s) 104, 382, 747, 1033
BstMBI GATC 5 cut(s) 208, 670, 918, 1086, 1216
BstMWI GCNNNNNNNGC 2 cut(s) 685, 816
BstNSI RCATGY 2 cut(s) 563, 1300
BstPI GGTNACC 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 1231
BstSFI CTRYAG 2 cut(s) 117, 1304
BstV1I GCAGC 4 cut(s) 283, 982, 1104, 1318
BstV2I GAAGAC 1 cut(s) 1279
BsuRI GGCC 4 cut(s) 72, 361, 704, 1231
BtgI CCRYGG 1 cut(s) 708
BtsCI GGATG 4 cut(s) 16, 1008, 1174, 1360
BtsIMutI CAGTG 3 cut(s) 1073, 1162, 1373
BveI ACCTGC 1 cut(s) 1191
CaiI CAGNNNCTG 1 cut(s) 652
Cfr10I RCCGGY 1 cut(s) 1254
Cfr13I GGNCC 1 cut(s) 702
CseI GACGC 1 cut(s) 154
Csp6I GTAC 6 cut(s) 328, 531, 595, 981, 1096, 1294
CspAI ACCGGT 1 cut(s) 1254
CviAII CATG 7 cut(s) 12, 560, 598, 709, 1144, 1291, 1297
CviQI GTAC 6 cut(s) 328, 531, 595, 981, 1096, 1294
DdeI CTNAG 7 cut(s) 386, 405, 640, 840, 1076, 1226, 1310
DpnI GATC 5 cut(s) 210, 672, 920, 1088, 1218
DpnII GATC 5 cut(s) 208, 670, 918, 1086, 1216
DriI GACNNNNNGTC 1 cut(s) 613
Eam1104I CTCTTC 3 cut(s) 107, 465, 585
Eam1105I GACNNNNNGTC 1 cut(s) 613
EarI CTCTTC 3 cut(s) 107, 465, 585
Ecl136II GAGCTC 1 cut(s) 1052
Eco130I CCWWGG 2 cut(s) 708, 1045
Eco147I AGGCCT 2 cut(s) 72, 361
Eco24I GRGCYC 1 cut(s) 1054
Eco32I GATATC 1 cut(s) 543
Eco53kI GAGCTC 1 cut(s) 1052
Eco57I CTGAAG 1 cut(s) 38
Eco91I GGTNACC 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 1052
EcoO109I RGGNCCY 1 cut(s) 702
EcoO65I GGTNACC 1 cut(s) 399
EcoRI GAATTC 1 cut(s) 1221
EcoRV GATATC 1 cut(s) 543
EcoT14I CCWWGG 2 cut(s) 708, 1045
EcoT38I GRGCYC 1 cut(s) 1054
ErhI CCWWGG 2 cut(s) 708, 1045
FaeI CATG 7 cut(s) 15, 563, 601, 712, 1147, 1294, 1300
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FatI CATG 7 cut(s) 11, 559, 597, 708, 1143, 1290, 1296
FauI CCCGC 1 cut(s) 1229
FauNDI CATATG 2 cut(s) 92, 146
FbaI TGATCA 1 cut(s) 918
FblI GTMKAC 1 cut(s) 1362
Fnu4HI GCNGC 4 cut(s) 272, 996, 1118, 1307
FokI GGATG 4 cut(s) 3, 1015, 1181, 1367
FriOI GRGCYC 1 cut(s) 1054
Fsp4HI GCNGC 4 cut(s) 272, 996, 1118, 1307
GluI GCNGC 4 cut(s) 272, 996, 1118, 1307
GsaI CCCAGC 2 cut(s) 35, 947
GsuI CTGGAG 1 cut(s) 362
HaeIII GGCC 4 cut(s) 72, 361, 704, 1231
HapII CCGG 2 cut(s) 1232, 1255
HgaI GACGC 1 cut(s) 154
Hin1I GRCGYC 2 cut(s) 165, 1284
Hin1II CATG 7 cut(s) 15, 563, 601, 712, 1147, 1294, 1300
HindIII AAGCTT 3 cut(s) 198, 241, 254
HinfI GANTC 5 cut(s) 260, 394, 740, 1108, 1147
HpaII CCGG 2 cut(s) 1232, 1255
Hpy166II GTNNAC 3 cut(s) 295, 715, 1363
Hpy188I TCNGA 5 cut(s) 57, 208, 387, 918, 1227
Hpy188III TCNNGA 3 cut(s) 341, 965, 1166
Hpy8I GTNNAC 3 cut(s) 295, 715, 1363
HpyAV CCTTC 6 cut(s) 176, 407, 430, 678, 862, 911
HpyCH4III ACNGT 3 cut(s) 665, 827, 1377
HpyCH4IV ACGT 1 cut(s) 1284
HpyF10VI GCNNNNNNNGC 2 cut(s) 685, 816
HpyF3I CTNAG 7 cut(s) 386, 405, 640, 840, 1076, 1226, 1310
HpySE526I ACGT 1 cut(s) 1284
Hsp92I GRCGYC 2 cut(s) 165, 1284
Hsp92II CATG 7 cut(s) 15, 563, 601, 712, 1147, 1294, 1300
Ksp22I TGATCA 1 cut(s) 918
Kzo9I GATC 5 cut(s) 208, 670, 918, 1086, 1216
LmnI GCTCC 5 cut(s) 442, 780, 902, 1049, 1057
Lsp1109I GCAGC 4 cut(s) 283, 982, 1104, 1318
LweI GCATC 1 cut(s) 645
MaeII ACGT 1 cut(s) 1284
MaeIII GTNAC 3 cut(s) 399, 886, 1018
MalI GATC 5 cut(s) 210, 672, 920, 1088, 1218
MboI GATC 5 cut(s) 208, 670, 918, 1086, 1216
MboII GAAGA 7 cut(s) 94, 295, 359, 482, 602, 1252, 1279
MfeI CAATTG 2 cut(s) 552, 1187
MhlI GDGCHC 2 cut(s) 376, 1054
MluCI AATT 6 cut(s) 81, 155, 320, 552, 1187, 1221
MlyI GAGTC 1 cut(s) 749
MmeI TCCRAC 2 cut(s) 490, 1359
MseI TTAA 7 cut(s) 41, 453, 660, 804, 879, 939, 1041
MslI CAYNNNNRTG 4 cut(s) 461, 564, 1155, 1295
MspA1I CMGCKG 2 cut(s) 266, 1309
MspI CCGG 2 cut(s) 1232, 1255
MspR9I CCNGG 1 cut(s) 1233
MunI CAATTG 2 cut(s) 552, 1187
MwoI GCNNNNNNNGC 2 cut(s) 685, 816
NciI CCSGG 1 cut(s) 1233
NcoI CCATGG 1 cut(s) 708
NdeI CATATG 2 cut(s) 92, 146
NdeII GATC 5 cut(s) 208, 670, 918, 1086, 1216
NlaIII CATG 7 cut(s) 15, 563, 601, 712, 1147, 1294, 1300
NlaIV GGNNCC 2 cut(s) 444, 988
NspI RCATGY 2 cut(s) 563, 1300
OliI CACNNNNGTG 1 cut(s) 461
PceI AGGCCT 2 cut(s) 72, 361
PciI ACATGT 1 cut(s) 1296
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 4 cut(s) 260, 394, 1108, 1147
PinAI ACCGGT 1 cut(s) 1254
PkrI GCNGC 4 cut(s) 273, 997, 1119, 1308
PleI GAGTC 1 cut(s) 748
PpsI GAGTC 1 cut(s) 748
PscI ACATGT 1 cut(s) 1296
PshBI ATTAAT 1 cut(s) 41
PsiI TTATAA 1 cut(s) 80
Psp124BI GAGCTC 1 cut(s) 1054
PspEI GGTNACC 1 cut(s) 399
PspFI CCCAGC 2 cut(s) 31, 943
PspN4I GGNNCC 2 cut(s) 444, 988
PspPI GGNCC 1 cut(s) 702
PstI CTGCAG 2 cut(s) 121, 1308
PstNI CAGNNNCTG 1 cut(s) 652
PvuII CAGCTG 2 cut(s) 266, 1309
RsaI GTAC 6 cut(s) 329, 532, 596, 982, 1097, 1295
RsaNI GTAC 6 cut(s) 328, 531, 595, 981, 1096, 1294
RseI CAYNNNNRTG 4 cut(s) 461, 564, 1155, 1295
SacI GAGCTC 1 cut(s) 1054
SaqAI TTAA 7 cut(s) 41, 453, 660, 804, 879, 939, 1041
SatI GCNGC 4 cut(s) 272, 996, 1118, 1307
Sau3AI GATC 5 cut(s) 208, 670, 918, 1086, 1216
Sau96I GGNCC 1 cut(s) 702
SchI GAGTC 1 cut(s) 749
ScrFI CCNGG 1 cut(s) 1233
SduI GDGCHC 2 cut(s) 376, 1054
SfaNI GCATC 1 cut(s) 645
SfcI CTRYAG 2 cut(s) 117, 1304
SmiMI CAYNNNNRTG 4 cut(s) 461, 564, 1155, 1295
Sse9I AATT 6 cut(s) 81, 155, 320, 552, 1187, 1221
SseBI AGGCCT 2 cut(s) 72, 361
SsiI CCGC 2 cut(s) 688, 1236
SspI AATATT 1 cut(s) 313
SstI GAGCTC 1 cut(s) 1054
StuI AGGCCT 2 cut(s) 72, 361
StyD4I CCNGG 1 cut(s) 1231
StyI CCWWGG 2 cut(s) 708, 1045
TaaI ACNGT 3 cut(s) 665, 827, 1377
TaiI ACGT 1 cut(s) 1287
TaqI TCGA 4 cut(s) 189, 211, 1209, 1219
TasI AATT 6 cut(s) 81, 155, 320, 552, 1187, 1221
TatI WGTACW 3 cut(s) 327, 594, 1293
TfiI GAWTC 4 cut(s) 260, 394, 1108, 1147
Tru1I TTAA 7 cut(s) 41, 453, 660, 804, 879, 939, 1041
Tru9I TTAA 7 cut(s) 41, 453, 660, 804, 879, 939, 1041
TscAI CASTG 3 cut(s) 1080, 1162, 1380
TseI GCWGC 4 cut(s) 271, 995, 1117, 1306
TspDTI ATGAA 3 cut(s) 449, 1139, 1279
TspRI CASTG 3 cut(s) 1080, 1162, 1380
VspI ATTAAT 1 cut(s) 41
XapI RAATTY 1 cut(s) 1221
XceI RCATGY 2 cut(s) 563, 1300
XcmI CCANNNNNNNNNTGG 2 cut(s) 28, 1162
XmiI GTMKAC 1 cut(s) 1362
ZraI GACGTC 1 cut(s) 1285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.