FvH4_6g06250

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012143
Physical Location & Seq
Reverse (-)
3577536 .. 3581776
4241 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g06250.t2

Sequence Viewer

Length: 1410 bp
ATGAGGAGGTCATGCCTTCCCAAGTGCAGATTTGGTAGGATAATGGGATGGCTCCAGATTCTACTGGGAGGGCTTGTTATAATTGTCAGCATATCCAGCCTCTTCAAGTTCTATTCTGCCGGATTTTTCTGGCGCAATGAAGACATATGCCGACATTTTTATGGGGTCAGAAATGAATATGAAGGATTCGACATAAAAGCTTTGTCTGATCGAGTAGGGGAAGTGCTAAACAAATTGGATAGTTTACAAGAAAAGCTCGAGTCAGCTGTGCAGCAAATGGAGAAGAACAAAGATGTCTTTCATACAACTAATATCACAAAATTGGAGTTCAAGAAGTATCTGGAGGAGGAAGTAATCAGGCCTCTTTATAGCGCTCACATTTCTCTGAGACTGATTCGGTTACCTATCCCAGAAGGTATTAGAAACTCAACAATGAAAGAGGAACCTTTGATTAATACTTTTGTGATTGAAGAGATAAGGAAGTATATAACCCCAAAGGAGAGCAGAATTGGGAAGATTAATATATTTGGTACAGAAAGGATATACAATACGATTGGACATGCTTGTGTGTTGTACAAGAAAGAGCTGGAAGAGTATATGAACTATGACATTGGGTCTTATTGTGACGATGATTGGAACTTGGCTCAGAAGCTGATGTTTAATGGTTGTGATCCTTTGCCCCGGAGGCGGTGCTTGACAAGGGCCTCACAGGTCTATCAGAAGCCTTATCCTATCAATGAGTCTCTGTGGAAATTGCCTGATGACAGAAATGTGCGGTGGAGCAATTATCAGTGCAGAAACTTTCAGTGCTTAATGAGCAACAACACCAAAAGGGGTTATTTAAAGTGCGTAGGATGTTTTGAAATGGAGAAGGAAAAGCTTAAATGGGTGATTAAGAATAGCTCGCTACCAGTTGATTTCCTGATCAGTGAAATTTTGGCTATTAAGCCTGGGGAGATAAGAATTGGTCTTGACTATGGTGTTGGCACAGGTACTTTTGCTGCAAGAATGAGAGAAAATAATGTTACAGTAGTCTCTACTGCTCTTAACCTTGGGGCTCCTTTCAATGAAATGATTGCCTTAAGAGGTTTGGTTCCGCTGTATATAACATTGAATCAGCGTCTACCTTTCTTTGATAACGTTATGGACTTGATTCACACAGCAGGGTTTCTGGATGGCTGGATTGACCTGCTGTTGCTGGATTTCATCCTGTTTGATTGGGACCGAGTTCTGAGGCCCGGGGGATTGTTATGGATCGACAGGTTCTTTTGCAATAAAAAGGATTTGGATGATTTCATGTACATGTTTCTGCAATTGAGATACAAGAAACACAAGTGGGCTATTACTCCTAAATCAAAGGATGAGGTCTACTTCTCAGCAGTGTTAGAGAAACCTCCAAGAGCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

470

Amino Acids

54.97

Weight (kDa)

8.88

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 309 - 418 1.5e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05390
fragaria_vesca FvH4_6g06250 FvH4_6g06250
malus_domestica MD12G1217000.v1.1
prunus_persica Prupe.6G323300_v2.0.a1
pyrus_communis pycom04g17960
rosa_chinensis RchiOBHm_Chr3g0455271
rosa_laevigata RLG00000025373
rosa_multiflora Rmu_sc0002169.1_g000037
rosa_roxburghii Rroxscaffold_6G00424410
rosa_rugosa Rorug03G0005500 Rorug03G0005600 Rorug03G0005700 Rorug03G0005800
rosa_samantha Rh3AG065600 Rh3BG067800 Rh3CG066200 Rh3DG067800
rosa_wichuraiana Rw3G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 1197
AccI GTMKAC 2 cut(s) 1123, 1368
AciI CCGC 3 cut(s) 688, 775, 1097
AclI AACGTT 1 cut(s) 1140
AclWI GGATC 2 cut(s) 665, 1262
AcsI RAATTY 1 cut(s) 933
AfaI GTAC 4 cut(s) 532, 575, 994, 1301
AfeI AGCGCT 1 cut(s) 373
AfiI CCNNNNNNNGG 1 cut(s) 687
AflII CTTAAG 1 cut(s) 1081
AflIII ACRYGT 1 cut(s) 1302
AgsI TTSAA 6 cut(s) 106, 331, 470, 863, 1066, 1114
AhdI GACNNNNNGTC 1 cut(s) 613
AjnI CCWGG 1 cut(s) 949
AluBI AGCT 8 cut(s) 200, 256, 266, 586, 652, 880, 903, 1403
AluI AGCT 8 cut(s) 200, 256, 266, 586, 652, 880, 903, 1403
Alw26I GTCTC 3 cut(s) 382, 747, 1039
AlwI GGATC 2 cut(s) 665, 1262
AlwNI CAGNNNCTG 1 cut(s) 652
Ama87I CYCGRG 2 cut(s) 257, 1238
Aor51HI AGCGCT 1 cut(s) 373
AoxI GGCC 3 cut(s) 359, 702, 1235
ApeKI GCWGC 2 cut(s) 271, 1001
ApoI RAATTY 1 cut(s) 933
AseI ATTAAT 2 cut(s) 453, 519
AspLEI GCGC 2 cut(s) 135, 374
AspS9I GGNCC 3 cut(s) 702, 1222, 1236
AsuC2I CCSGG 3 cut(s) 682, 1239, 1240
AsuHPI GGTGA 1 cut(s) 901
AvaI CYCGRG 2 cut(s) 257, 1238
AvaII GGWCC 1 cut(s) 1222
BanII GRGCYC 1 cut(s) 1060
BbsI GAAGAC 1 cut(s) 147
BbvI GCAGC 2 cut(s) 283, 988
BccI CCATC 2 cut(s) 42, 1169
BciT130I CCWGG 1 cut(s) 951
BclI TGATCA 1 cut(s) 924
BcnI CCSGG 3 cut(s) 682, 1239, 1240
BcoDI GTCTC 3 cut(s) 382, 747, 1039
BfoI RGCGCY 1 cut(s) 375
BfrI CTTAAG 1 cut(s) 1081
BfuAI ACCTGC 1 cut(s) 1197
BglI GCCNNNNNGGC 1 cut(s) 685
BisI GCNGC 2 cut(s) 272, 1002
BlsI GCNGC 2 cut(s) 273, 1003
Bme1390I CCNGG 4 cut(s) 682, 951, 1239, 1240
Bme18I GGWCC 1 cut(s) 1222
BmeRI GACNNNNNGTC 1 cut(s) 613
BmeT110I CYCGRG 2 cut(s) 257, 1238
BmgT120I GGNCC 3 cut(s) 702, 1222, 1236
BmiI GGNNCC 5 cut(s) 53, 444, 1059, 1095, 1223
BmrFI CCNGG 4 cut(s) 682, 951, 1239, 1240
BmrI ACTGGG 1 cut(s) 74
BmuI ACTGGG 1 cut(s) 74
BpiI GAAGAC 1 cut(s) 147
BpmI CTGGAG 2 cut(s) 38, 362
BpuMI CCSGG 3 cut(s) 682, 1239, 1240
BsaJI CCNNGG 5 cut(s) 680, 950, 1051, 1238, 1239
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 1 cut(s) 687
Bse1I ACTGG 2 cut(s) 69, 911
Bse3DI GCAATG 1 cut(s) 142
BseBI CCWGG 1 cut(s) 951
BseDI CCNNGG 5 cut(s) 680, 950, 1051, 1238, 1239
BseGI GGATG 6 cut(s) 53, 860, 1180, 1206, 1294, 1366
BseLI CCNNNNNNNGG 1 cut(s) 687
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 4 cut(s) 377, 659, 1223, 1389
BseNI ACTGG 2 cut(s) 69, 911
BseRI GAGGAG 2 cut(s) 19, 359
BseXI GCAGC 2 cut(s) 283, 988
BsgI GTGCAG 3 cut(s) 46, 290, 814
BshFI GGCC 3 cut(s) 361, 704, 1237
BsiHKCI CYCGRG 2 cut(s) 257, 1238
BsiSI CCGG 3 cut(s) 120, 682, 1239
BslFI GGGAC 1 cut(s) 1235
BslI CCNNNNNNNGG 1 cut(s) 687
BsmAI GTCTC 3 cut(s) 382, 747, 1039
BsmFI GGGAC 1 cut(s) 1235
BsnI GGCC 3 cut(s) 361, 704, 1237
BsoBI CYCGRG 2 cut(s) 257, 1238
Bsp1286I GDGCHC 1 cut(s) 1060
Bsp1407I TGTACA 2 cut(s) 573, 1299
Bsp143I GATC 4 cut(s) 208, 670, 924, 1254
BspACI CCGC 3 cut(s) 688, 775, 1097
BspANI GGCC 3 cut(s) 361, 704, 1237
BspCNI CTCAG 4 cut(s) 378, 658, 1224, 1388
BspLI GGNNCC 5 cut(s) 53, 444, 1059, 1095, 1223
BspMI ACCTGC 1 cut(s) 1197
BspPI GGATC 2 cut(s) 665, 1262
BspTI CTTAAG 1 cut(s) 1081
BsrDI GCAATG 1 cut(s) 142
BsrGI TGTACA 2 cut(s) 573, 1299
BsrI ACTGG 2 cut(s) 69, 911
BssECI CCNNGG 5 cut(s) 680, 950, 1051, 1238, 1239
BssMI GATC 4 cut(s) 208, 670, 924, 1254
BssT1I CCWWGG 1 cut(s) 1051
Bst2UI CCWGG 1 cut(s) 951
Bst4CI ACNGT 1 cut(s) 1030
Bst6I CTCTTC 3 cut(s) 107, 465, 585
BstAFI CTTAAG 1 cut(s) 1081
BstAUI TGTACA 2 cut(s) 573, 1299
BstC8I GCNNGC 1 cut(s) 905
BstDEI CTNAG 4 cut(s) 386, 645, 1232, 1375
BstEII GGTNACC 1 cut(s) 399
BstF5I GGATG 6 cut(s) 53, 860, 1180, 1206, 1294, 1366
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 2 cut(s) 135, 374
BstKTI GATC 4 cut(s) 211, 673, 927, 1257
BstMAI GTCTC 3 cut(s) 382, 747, 1039
BstMBI GATC 4 cut(s) 208, 670, 924, 1254
BstMWI GCNNNNNNNGC 3 cut(s) 96, 685, 816
BstNI CCWGG 1 cut(s) 951
BstNSI RCATGY 2 cut(s) 563, 1306
BstPI GGTNACC 1 cut(s) 399
BstSCI CCNGG 4 cut(s) 680, 949, 1237, 1238
BstV1I GCAGC 2 cut(s) 283, 988
BstV2I GAAGAC 1 cut(s) 147
BsuRI GGCC 3 cut(s) 361, 704, 1237
BtsCI GGATG 6 cut(s) 53, 860, 1180, 1206, 1294, 1366
BtsI GCAGTG 1 cut(s) 1386
BtsIMutI CAGTG 4 cut(s) 797, 812, 934, 1386
BveI ACCTGC 1 cut(s) 1197
Cac8I GCNNGC 1 cut(s) 905
CaiI CAGNNNCTG 1 cut(s) 652
CfoI GCGC 2 cut(s) 135, 374
Cfr13I GGNCC 3 cut(s) 702, 1222, 1236
Cfr9I CCCGGG 1 cut(s) 1238
CseI GACGC 1 cut(s) 1109
Csp6I GTAC 4 cut(s) 531, 574, 993, 1300
CviAII CATG 4 cut(s) 12, 560, 1297, 1303
CviQI GTAC 4 cut(s) 531, 574, 993, 1300
DdeI CTNAG 4 cut(s) 386, 645, 1232, 1375
DpnI GATC 4 cut(s) 210, 672, 926, 1256
DpnII GATC 4 cut(s) 208, 670, 924, 1254
DraI TTTAAA 1 cut(s) 843
DriI GACNNNNNGTC 1 cut(s) 613
Eam1104I CTCTTC 3 cut(s) 107, 465, 585
Eam1105I GACNNNNNGTC 1 cut(s) 613
EarI CTCTTC 3 cut(s) 107, 465, 585
Eco130I CCWWGG 1 cut(s) 1051
Eco147I AGGCCT 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 1060
Eco47I GGWCC 1 cut(s) 1222
Eco47III AGCGCT 1 cut(s) 373
Eco88I CYCGRG 2 cut(s) 257, 1238
Eco91I GGTNACC 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 702
EcoO65I GGTNACC 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 949
EcoT14I CCWWGG 1 cut(s) 1051
EcoT38I GRGCYC 1 cut(s) 1060
ErhI CCWWGG 1 cut(s) 1051
FaeI CATG 4 cut(s) 15, 563, 1300, 1306
FaqI GGGAC 1 cut(s) 1235
FatI CATG 4 cut(s) 11, 559, 1296, 1302
FauNDI CATATG 1 cut(s) 146
FbaI TGATCA 1 cut(s) 924
FblI GTMKAC 2 cut(s) 1123, 1368
Fnu4HI GCNGC 2 cut(s) 272, 1002
FokI GGATG 6 cut(s) 60, 867, 1187, 1193, 1301, 1373
FriOI GRGCYC 1 cut(s) 1060
Fsp4HI GCNGC 2 cut(s) 272, 1002
GlaI GCGC 2 cut(s) 134, 373
GluI GCNGC 2 cut(s) 272, 1002
GsuI CTGGAG 2 cut(s) 38, 362
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 3 cut(s) 361, 704, 1237
HapII CCGG 3 cut(s) 120, 682, 1239
HgaI GACGC 1 cut(s) 1109
HhaI GCGC 2 cut(s) 135, 374
Hin1II CATG 4 cut(s) 15, 563, 1300, 1306
Hin6I GCGC 2 cut(s) 133, 372
HinP1I GCGC 2 cut(s) 133, 372
HindIII AAGCTT 2 cut(s) 198, 878
HinfI GANTC 7 cut(s) 58, 186, 260, 394, 740, 1114, 1153
HpaII CCGG 3 cut(s) 120, 682, 1239
HphI GGTGA 1 cut(s) 901
Hpy166II GTNNAC 3 cut(s) 245, 1124, 1369
Hpy188I TCNGA 7 cut(s) 170, 208, 387, 648, 720, 1233, 1409
Hpy188III TCNNGA 6 cut(s) 55, 331, 341, 922, 971, 1172
Hpy8I GTNNAC 3 cut(s) 245, 1124, 1369
HpyAV CCTTC 4 cut(s) 26, 176, 407, 865
HpyCH4III ACNGT 1 cut(s) 1030
HpyCH4IV ACGT 1 cut(s) 1140
HpyCH4V TGCA 6 cut(s) 27, 271, 795, 1004, 1272, 1312
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 685, 816
HpyF3I CTNAG 4 cut(s) 386, 645, 1232, 1375
HpySE526I ACGT 1 cut(s) 1140
Hsp92II CATG 4 cut(s) 15, 563, 1300, 1306
HspAI GCGC 2 cut(s) 133, 372
Ksp22I TGATCA 1 cut(s) 924
Kzo9I GATC 4 cut(s) 208, 670, 924, 1254
LmnI GCTCC 3 cut(s) 57, 780, 1063
Lsp1109I GCAGC 2 cut(s) 283, 988
MaeII ACGT 1 cut(s) 1140
MaeIII GTNAC 3 cut(s) 399, 623, 1024
MalI GATC 4 cut(s) 210, 672, 926, 1256
MboI GATC 4 cut(s) 208, 670, 924, 1254
MboII GAAGA 6 cut(s) 94, 152, 295, 482, 526, 602
MfeI CAATTG 1 cut(s) 1313
MhlI GDGCHC 1 cut(s) 1060
MluCI AATT 9 cut(s) 81, 233, 320, 507, 752, 784, 933, 963, 1313
MlyI GAGTC 2 cut(s) 269, 749
MslI CAYNNNNRTG 3 cut(s) 159, 564, 1301
MspA1I CMGCKG 2 cut(s) 266, 1099
MspCI CTTAAG 1 cut(s) 1081
MspI CCGG 3 cut(s) 120, 682, 1239
MspR9I CCNGG 4 cut(s) 682, 951, 1239, 1240
MunI CAATTG 1 cut(s) 1313
MvaI CCWGG 1 cut(s) 951
MwoI GCNNNNNNNGC 3 cut(s) 96, 685, 816
NciI CCSGG 3 cut(s) 682, 1239, 1240
NdeI CATATG 1 cut(s) 146
NdeII GATC 4 cut(s) 208, 670, 924, 1254
NlaIII CATG 4 cut(s) 15, 563, 1300, 1306
NlaIV GGNNCC 5 cut(s) 53, 444, 1059, 1095, 1223
NmuCI GTSAC 1 cut(s) 623
NspI RCATGY 2 cut(s) 563, 1306
PaeR7I CTCGAG 1 cut(s) 257
PceI AGGCCT 1 cut(s) 361
PciI ACATGT 1 cut(s) 1302
PfeI GAWTC 5 cut(s) 58, 186, 394, 1114, 1153
PkrI GCNGC 2 cut(s) 273, 1003
PleI GAGTC 2 cut(s) 268, 748
PpsI GAGTC 2 cut(s) 268, 748
PscI ACATGT 1 cut(s) 1302
PshBI ATTAAT 2 cut(s) 453, 519
PsiI TTATAA 1 cut(s) 80
Psp1406I AACGTT 1 cut(s) 1140
Psp6I CCWGG 1 cut(s) 949
PspEI GGTNACC 1 cut(s) 399
PspGI CCWGG 1 cut(s) 949
PspN4I GGNNCC 5 cut(s) 53, 444, 1059, 1095, 1223
PspPI GGNCC 3 cut(s) 702, 1222, 1236
PspXI VCTCGAGB 1 cut(s) 257
PstNI CAGNNNCTG 1 cut(s) 652
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 4 cut(s) 532, 575, 994, 1301
RsaNI GTAC 4 cut(s) 531, 574, 993, 1300
RseI CAYNNNNRTG 3 cut(s) 159, 564, 1301
SatI GCNGC 2 cut(s) 272, 1002
Sau3AI GATC 4 cut(s) 208, 670, 924, 1254
Sau96I GGNCC 3 cut(s) 702, 1222, 1236
SchI GAGTC 2 cut(s) 269, 749
ScrFI CCNGG 4 cut(s) 682, 951, 1239, 1240
SduI GDGCHC 1 cut(s) 1060
Sfr274I CTCGAG 1 cut(s) 257
SinI GGWCC 1 cut(s) 1222
SlaI CTCGAG 1 cut(s) 257
SmaI CCCGGG 1 cut(s) 1240
SmiMI CAYNNNNRTG 3 cut(s) 159, 564, 1301
SmlI CTYRAG 2 cut(s) 257, 1081
SmoI CTYRAG 2 cut(s) 257, 1081
Sse9I AATT 9 cut(s) 81, 233, 320, 507, 752, 784, 933, 963, 1313
SseBI AGGCCT 1 cut(s) 361
SsiI CCGC 3 cut(s) 688, 775, 1097
StuI AGGCCT 1 cut(s) 361
StyD4I CCNGG 4 cut(s) 680, 949, 1237, 1238
StyI CCWWGG 1 cut(s) 1051
TaaI ACNGT 1 cut(s) 1030
TaiI ACGT 1 cut(s) 1143
TaqI TCGA 4 cut(s) 189, 211, 258, 1257
TaqII GACCGA 1 cut(s) 1239
TasI AATT 9 cut(s) 81, 233, 320, 507, 752, 784, 933, 963, 1313
TatI WGTACW 2 cut(s) 573, 1299
TfiI GAWTC 5 cut(s) 58, 186, 394, 1114, 1153
TscAI CASTG 4 cut(s) 797, 812, 934, 1386
TseFI GTSAC 1 cut(s) 623
TseI GCWGC 2 cut(s) 271, 1001
Tsp45I GTSAC 1 cut(s) 623
TspDTI ATGAA 9 cut(s) 153, 189, 195, 290, 449, 614, 1083, 1195, 1285
TspMI CCCGGG 1 cut(s) 1238
TspRI CASTG 4 cut(s) 797, 812, 934, 1386
Vha464I CTTAAG 1 cut(s) 1081
VpaK11BI GGWCC 1 cut(s) 1222
VspI ATTAAT 2 cut(s) 453, 519
XapI RAATTY 1 cut(s) 933
XceI RCATGY 2 cut(s) 563, 1306
XhoI CTCGAG 1 cut(s) 257
XmaI CCCGGG 1 cut(s) 1238
XmiI GTMKAC 2 cut(s) 1123, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.