RLG00000025373

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012143
Physical Location & Seq
Forward (+)
45377571 .. 45380258
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025373

Sequence Viewer

Length: 1482 bp
ATGGAGATTCGTGCTTCGAAGTCTCTTGAGTTCTTGTGGGACAGAGTTGGAGTGTGTGTTAAATTTTGCACAGGAAGATGGGTTTGGCTCCAGCTTGATGCAAGTTCCTGGAGATTCTTCATCTGGGTTGAAGAACTGAGAACAGAGAGGACGGAATTAGACATATCCAGCCTCTTCAAGTTCTACTCTGGTGGATTTTTCTTGCGCAATGAAGACATATGCCGACATGTTTATGGGGTCAGAGATGAATATGGGGGCTTCGACATAAAAGCTCTGTCTGATCGAGTAGGGGAAGTGCTAAACAAATTGGATAGTTTACAAGAAAAGCTTGAGTCAGCTGTGCAGCAAATGGAGAAGAACAAAGATGTCTTGCATACAACTAATATCACAAAACTGGAGTTCAAGAAGTATCTGGAGGAGCAAGTAATCAGGCCTCTTTATAGTGCTCACATTTCTCTGAGACTGATTCGGTTACCTAACCCAGAAGGTATTAGAAACTCAACAATGAAAGAGGAACCTTTGATTAATACTTTTGTGATTGAAGAGATAAGGAAGTATATAACCCCAAAGGAGAGTAGAATAGGGAAGATTAATATATATGGTACAGAGAGGATATACAATACGATTGGACATGCTTGTGTGTTGTACAAGAAAGAGTTGGAAGAGTACATGAACTATGACATCGGATCTTATTGTAACGATGATTGGAACTTGGCTCAGAAGCTGATGTTAAATGGTTGTGATCCTTTGCCGCGGAGGCGATGTTTGACAAGGGCCTCAAAGGTCTATCAGAAGCCTTATCCGATCAATGACTCTCTGTGGAAATTGCCGGATGACAGAAATGTGAGGTGGAGCAATTATCAGTGCAGGAACTTTCAGTGCTTGATGAGCAATAACTCCAAGAGGGGTTATTCAAAGTGCGTAGGATGTTTTGAAATGGAGAAGGAAAAGCTTAAATGGATGATTACTAATAAATTGCTACCAGTTGATTTCCTGATCAGTGAAATTTTGGCCATTAAGCCTGGGGAGATAAGAATTGGTCTTGACTTTGGTGTTGGCACAGGAACTTATGCTGCAAGAATGAGAGAAAATAATGTTACAGTAGTCTCTACTGCTCTTAACCTTGGGGCTCCTTTCAATGAAATGATTGCATTAAGAGGTTTGGTTCCGCTGTATGTAACATTGAATCAGCGTCTACCTTTCTTTGATAATATAATGGACTTGATTCATACCACTGGGTTTCTGGATGGCTGGATTGACCTGCAGTTGCTGGATTTCATCCTGTTTGATTGGGACCGAATTCTGAGGCCCGGCGGATTGTTATGGATTGACCGGTTCTTCTGCAATAAAAAGGATTTGGATGATTTCATGTATATGTTTCTGCAGTTGAGATACAAGAAACACAAGTGGGTTATTTCTCCCAAATCAAAGGATGAGGTCTACTTTTCTGCAGTGTTAGAGAAACCTCCAAGAGCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

494

Amino Acids

58.01

Weight (kDa)

8.64

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 340 - 442 7.8e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05390
fragaria_vesca FvH4_6g06250 FvH4_6g06250
malus_domestica MD12G1217000.v1.1
prunus_persica Prupe.6G323300_v2.0.a1
pyrus_communis pycom04g17960
rosa_chinensis RchiOBHm_Chr3g0455271
rosa_laevigata RLG00000025373
rosa_multiflora Rmu_sc0002169.1_g000037
rosa_roxburghii Rroxscaffold_6G00424410
rosa_rugosa Rorug03G0005500 Rorug03G0005600 Rorug03G0005700 Rorug03G0005800
rosa_samantha Rh3AG065600 Rh3BG067800 Rh3CG066200 Rh3DG067800
rosa_wichuraiana Rw3G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 1269
AccI GTMKAC 2 cut(s) 1195, 1440
AccII CGCG 1 cut(s) 754
AciI CCGC 4 cut(s) 752, 754, 1169, 1314
AclWI GGATC 2 cut(s) 694, 737
AcoI YGGCCR 1 cut(s) 1011
AcsI RAATTY 3 cut(s) 62, 1005, 1299
AfaI GTAC 3 cut(s) 604, 647, 668
AflIII ACRYGT 1 cut(s) 226
AgeI ACCGGT 1 cut(s) 1332
AgsI TTSAA 8 cut(s) 131, 178, 403, 542, 915, 935, 1138, 1186
AjnI CCWGG 2 cut(s) 107, 1021
AjuI GAANNNNNNNTTGG 2 cut(s) 67, 99
AluBI AGCT 6 cut(s) 94, 272, 328, 338, 724, 952
AluI AGCT 6 cut(s) 94, 272, 328, 338, 724, 952
Alw21I GWGCWC 1 cut(s) 448
Alw26I GTCTC 3 cut(s) 27, 454, 1111
AlwI GGATC 2 cut(s) 694, 737
AlwNI CAGNNNCTG 2 cut(s) 724, 1270
AoxI GGCC 4 cut(s) 431, 774, 1011, 1307
ApeKI GCWGC 2 cut(s) 343, 1073
ApoI RAATTY 3 cut(s) 62, 1005, 1299
AseI ATTAAT 2 cut(s) 525, 591
AsiGI ACCGGT 1 cut(s) 1332
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 3 cut(s) 774, 1294, 1308
AsuC2I CCSGG 1 cut(s) 1311
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 1 cut(s) 1294
BalI TGGCCA 1 cut(s) 1013
BanII GRGCYC 1 cut(s) 1132
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 448
BbvI GCAGC 2 cut(s) 355, 1060
BccI CCATC 2 cut(s) 72, 1241
BciT130I CCWGG 2 cut(s) 109, 1023
BclI TGATCA 1 cut(s) 996
BcnI CCSGG 1 cut(s) 1311
BcoDI GTCTC 3 cut(s) 27, 454, 1111
BfmI CTRYAG 3 cut(s) 1262, 1382, 1449
BfuAI ACCTGC 1 cut(s) 1269
BglI GCCNNNNNGGC 1 cut(s) 757
BisI GCNGC 3 cut(s) 344, 752, 1074
BlsI GCNGC 3 cut(s) 345, 753, 1075
Bme1390I CCNGG 3 cut(s) 109, 1023, 1311
Bme18I GGWCC 1 cut(s) 1294
BmgT120I GGNCC 3 cut(s) 774, 1294, 1308
BmiI GGNNCC 5 cut(s) 89, 516, 1131, 1167, 1295
BmrFI CCNGG 3 cut(s) 109, 1023, 1311
BmrI ACTGGG 1 cut(s) 1245
BmsI GCATC 1 cut(s) 88
BmuI ACTGGG 1 cut(s) 1245
BpiI GAAGAC 1 cut(s) 219
BpmI CTGGAG 4 cut(s) 74, 130, 416, 434
Bpu14I TTCGAA 1 cut(s) 17
BpuEI CTTGAG 2 cut(s) 47, 350
BpuMI CCSGG 1 cut(s) 1311
BsaJI CCNNGG 3 cut(s) 752, 1022, 1123
BsaWI WCCGGW 1 cut(s) 1332
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 748, 778
Bse118I RCCGGY 1 cut(s) 1332
Bse1I ACTGG 3 cut(s) 399, 983, 1240
Bse3DI GCAATG 1 cut(s) 214
BseBI CCWGG 2 cut(s) 109, 1023
BseDI CCNNGG 3 cut(s) 752, 1022, 1123
BseGI GGATG 7 cut(s) 838, 932, 966, 1252, 1278, 1366, 1438
BseMI GCAATG 1 cut(s) 214
BseMII CTCAG 4 cut(s) 128, 449, 731, 1295
BseNI ACTGG 3 cut(s) 399, 983, 1240
BseRI GAGGAG 1 cut(s) 431
BseXI GCAGC 2 cut(s) 355, 1060
BsgI GTGCAG 2 cut(s) 362, 886
Bsh1236I CGCG 1 cut(s) 754
BshFI GGCC 4 cut(s) 433, 776, 1013, 1309
BshTI ACCGGT 1 cut(s) 1332
BsiHKAI GWGCWC 1 cut(s) 448
BsiSI CCGG 3 cut(s) 830, 1311, 1333
BslFI GGGAC 2 cut(s) 53, 1307
BsmAI GTCTC 3 cut(s) 27, 454, 1111
BsmFI GGGAC 2 cut(s) 53, 1307
BsnI GGCC 4 cut(s) 433, 776, 1013, 1309
Bsp119I TTCGAA 1 cut(s) 17
Bsp1286I GDGCHC 2 cut(s) 448, 1132
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 5 cut(s) 280, 686, 742, 804, 996
BspACI CCGC 4 cut(s) 752, 754, 1169, 1314
BspANI GGCC 4 cut(s) 433, 776, 1013, 1309
BspCNI CTCAG 4 cut(s) 129, 450, 730, 1296
BspFNI CGCG 1 cut(s) 754
BspLI GGNNCC 5 cut(s) 89, 516, 1131, 1167, 1295
BspMAI CTGCAG 3 cut(s) 1266, 1386, 1453
BspMI ACCTGC 1 cut(s) 1269
BspPI GGATC 2 cut(s) 694, 737
BspT104I TTCGAA 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 1332
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 3 cut(s) 399, 983, 1240
BssAI RCCGGY 1 cut(s) 1332
BssECI CCNNGG 3 cut(s) 752, 1022, 1123
BssMI GATC 5 cut(s) 280, 686, 742, 804, 996
BssT1I CCWWGG 1 cut(s) 1123
Bst2UI CCWGG 2 cut(s) 109, 1023
Bst4CI ACNGT 1 cut(s) 1102
Bst6I CTCTTC 3 cut(s) 179, 537, 657
BstAUI TGTACA 1 cut(s) 645
BstBI TTCGAA 1 cut(s) 17
BstDEI CTNAG 4 cut(s) 137, 458, 717, 1304
BstDSI CCRYGG 1 cut(s) 752
BstEII GGTNACC 1 cut(s) 471
BstF5I GGATG 7 cut(s) 838, 932, 966, 1252, 1278, 1366, 1438
BstFNI CGCG 1 cut(s) 754
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 5 cut(s) 283, 689, 745, 807, 999
BstMAI GTCTC 3 cut(s) 27, 454, 1111
BstMBI GATC 5 cut(s) 280, 686, 742, 804, 996
BstMWI GCNNNNNNNGC 2 cut(s) 757, 888
BstNI CCWGG 2 cut(s) 109, 1023
BstNSI RCATGY 2 cut(s) 230, 635
BstPI GGTNACC 1 cut(s) 471
BstSCI CCNGG 3 cut(s) 107, 1021, 1309
BstSFI CTRYAG 3 cut(s) 1262, 1382, 1449
BstUI CGCG 1 cut(s) 754
BstV1I GCAGC 2 cut(s) 355, 1060
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 686
BstYI RGATCY 1 cut(s) 686
BsuRI GGCC 4 cut(s) 433, 776, 1013, 1309
BtgI CCRYGG 1 cut(s) 752
BtgZI GCGATG 1 cut(s) 775
BtsCI GGATG 7 cut(s) 838, 932, 966, 1252, 1278, 1366, 1438
BtsI GCAGTG 1 cut(s) 1458
BtsIMutI CAGTG 5 cut(s) 869, 884, 1006, 1233, 1458
BveI ACCTGC 1 cut(s) 1269
CaiI CAGNNNCTG 2 cut(s) 724, 1270
CfoI GCGC 1 cut(s) 207
Cfr10I RCCGGY 1 cut(s) 1332
Cfr13I GGNCC 3 cut(s) 774, 1294, 1308
Cfr42I CCGCGG 1 cut(s) 755
CseI GACGC 1 cut(s) 1181
Csp6I GTAC 3 cut(s) 603, 646, 667
CspAI ACCGGT 1 cut(s) 1332
CviAII CATG 4 cut(s) 227, 632, 670, 1369
CviQI GTAC 3 cut(s) 603, 646, 667
DdeI CTNAG 4 cut(s) 137, 458, 717, 1304
DpnI GATC 5 cut(s) 282, 688, 744, 806, 998
DpnII GATC 5 cut(s) 280, 686, 742, 804, 996
EaeI YGGCCR 1 cut(s) 1011
Eam1104I CTCTTC 3 cut(s) 179, 537, 657
EarI CTCTTC 3 cut(s) 179, 537, 657
EciI GGCGGA 1 cut(s) 1329
Eco130I CCWWGG 1 cut(s) 1123
Eco147I AGGCCT 1 cut(s) 433
Eco24I GRGCYC 1 cut(s) 1132
Eco47I GGWCC 1 cut(s) 1294
Eco91I GGTNACC 1 cut(s) 471
EcoO109I RGGNCCY 1 cut(s) 774
EcoO65I GGTNACC 1 cut(s) 471
EcoRI GAATTC 1 cut(s) 1299
EcoRII CCWGG 2 cut(s) 107, 1021
EcoT14I CCWWGG 1 cut(s) 1123
EcoT38I GRGCYC 1 cut(s) 1132
ErhI CCWWGG 1 cut(s) 1123
FaeI CATG 4 cut(s) 230, 635, 673, 1372
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 2 cut(s) 53, 1307
FatI CATG 4 cut(s) 226, 631, 669, 1368
FauNDI CATATG 1 cut(s) 218
FbaI TGATCA 1 cut(s) 996
FblI GTMKAC 2 cut(s) 1195, 1440
Fnu4HI GCNGC 3 cut(s) 344, 752, 1074
FokI GGATG 7 cut(s) 845, 939, 973, 1259, 1265, 1373, 1445
FriOI GRGCYC 1 cut(s) 1132
Fsp4HI GCNGC 3 cut(s) 344, 752, 1074
FspI TGCGCA 1 cut(s) 206
GlaI GCGC 1 cut(s) 206
GluI GCNGC 3 cut(s) 344, 752, 1074
GsuI CTGGAG 4 cut(s) 74, 130, 416, 434
HaeIII GGCC 4 cut(s) 433, 776, 1013, 1309
HapII CCGG 3 cut(s) 830, 1311, 1333
HgaI GACGC 1 cut(s) 1181
HhaI GCGC 1 cut(s) 207
Hin1II CATG 4 cut(s) 230, 635, 673, 1372
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HindIII AAGCTT 2 cut(s) 326, 950
HinfI GANTC 7 cut(s) 7, 114, 332, 466, 812, 1186, 1225
HpaII CCGG 3 cut(s) 830, 1311, 1333
Hpy166II GTNNAC 3 cut(s) 317, 1196, 1441
Hpy188I TCNGA 9 cut(s) 242, 280, 459, 686, 720, 792, 804, 1305, 1481
Hpy188III TCNNGA 6 cut(s) 26, 403, 413, 994, 1043, 1244
Hpy8I GTNNAC 3 cut(s) 317, 1196, 1441
HpyAV CCTTC 2 cut(s) 479, 937
HpyCH4III ACNGT 1 cut(s) 1102
HpyF10VI GCNNNNNNNGC 2 cut(s) 757, 888
HpyF3I CTNAG 4 cut(s) 137, 458, 717, 1304
Hsp92II CATG 4 cut(s) 230, 635, 673, 1372
HspAI GCGC 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 996
KspI CCGCGG 1 cut(s) 755
Kzo9I GATC 5 cut(s) 280, 686, 742, 804, 996
LmnI GCTCC 4 cut(s) 93, 418, 852, 1135
Lsp1109I GCAGC 2 cut(s) 355, 1060
LweI GCATC 1 cut(s) 88
MaeIII GTNAC 4 cut(s) 471, 695, 1096, 1177
MalI GATC 5 cut(s) 282, 688, 744, 806, 998
MboI GATC 5 cut(s) 280, 686, 742, 804, 996
MflI RGATCY 1 cut(s) 686
MhlI GDGCHC 2 cut(s) 448, 1132
MlsI TGGCCA 1 cut(s) 1013
MluCI AATT 9 cut(s) 62, 155, 305, 824, 856, 974, 1005, 1035, 1299
MluNI TGGCCA 1 cut(s) 1013
MlyI GAGTC 2 cut(s) 341, 806
MmeI TCCRAC 2 cut(s) 28, 639
Mox20I TGGCCA 1 cut(s) 1013
MscI TGGCCA 1 cut(s) 1013
MseI TTAA 8 cut(s) 60, 525, 591, 731, 954, 1017, 1119, 1154
MslI CAYNNNNRTG 3 cut(s) 231, 636, 1373
Msp20I TGGCCA 1 cut(s) 1013
MspA1I CMGCKG 3 cut(s) 338, 754, 1171
MspI CCGG 3 cut(s) 830, 1311, 1333
MspR9I CCNGG 3 cut(s) 109, 1023, 1311
MvaI CCWGG 2 cut(s) 109, 1023
MvnI CGCG 1 cut(s) 754
MwoI GCNNNNNNNGC 2 cut(s) 757, 888
NciI CCSGG 1 cut(s) 1311
NdeI CATATG 1 cut(s) 218
NdeII GATC 5 cut(s) 280, 686, 742, 804, 996
NlaIII CATG 4 cut(s) 230, 635, 673, 1372
NlaIV GGNNCC 5 cut(s) 89, 516, 1131, 1167, 1295
NsbI TGCGCA 1 cut(s) 206
NspI RCATGY 2 cut(s) 230, 635
NspV TTCGAA 1 cut(s) 17
PceI AGGCCT 1 cut(s) 433
PciI ACATGT 1 cut(s) 226
PfeI GAWTC 5 cut(s) 7, 114, 466, 1186, 1225
PfoI TCCNGGA 1 cut(s) 107
PinAI ACCGGT 1 cut(s) 1332
PkrI GCNGC 3 cut(s) 345, 753, 1075
PleI GAGTC 2 cut(s) 340, 806
PpsI GAGTC 2 cut(s) 340, 806
PscI ACATGT 1 cut(s) 226
PshBI ATTAAT 2 cut(s) 525, 591
Psp6I CCWGG 2 cut(s) 107, 1021
PspEI GGTNACC 1 cut(s) 471
PspGI CCWGG 2 cut(s) 107, 1021
PspN4I GGNNCC 5 cut(s) 89, 516, 1131, 1167, 1295
PspPI GGNCC 3 cut(s) 774, 1294, 1308
PstI CTGCAG 3 cut(s) 1266, 1386, 1453
PstNI CAGNNNCTG 2 cut(s) 724, 1270
PsuI RGATCY 1 cut(s) 686
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 3 cut(s) 604, 647, 668
RsaNI GTAC 3 cut(s) 603, 646, 667
RseI CAYNNNNRTG 3 cut(s) 231, 636, 1373
SacII CCGCGG 1 cut(s) 755
SaqAI TTAA 8 cut(s) 60, 525, 591, 731, 954, 1017, 1119, 1154
SatI GCNGC 3 cut(s) 344, 752, 1074
Sau3AI GATC 5 cut(s) 280, 686, 742, 804, 996
Sau96I GGNCC 3 cut(s) 774, 1294, 1308
SchI GAGTC 2 cut(s) 341, 806
ScrFI CCNGG 3 cut(s) 109, 1023, 1311
SduI GDGCHC 2 cut(s) 448, 1132
SfaNI GCATC 1 cut(s) 88
SfcI CTRYAG 3 cut(s) 1262, 1382, 1449
Sfr303I CCGCGG 1 cut(s) 755
SfuI TTCGAA 1 cut(s) 17
SgrBI CCGCGG 1 cut(s) 755
SinI GGWCC 1 cut(s) 1294
SmiMI CAYNNNNRTG 3 cut(s) 231, 636, 1373
SmlI CTYRAG 2 cut(s) 26, 329
SmoI CTYRAG 2 cut(s) 26, 329
Sse9I AATT 9 cut(s) 62, 155, 305, 824, 856, 974, 1005, 1035, 1299
SseBI AGGCCT 1 cut(s) 433
SsiI CCGC 4 cut(s) 752, 754, 1169, 1314
StuI AGGCCT 1 cut(s) 433
StyD4I CCNGG 3 cut(s) 107, 1021, 1309
StyI CCWWGG 1 cut(s) 1123
TaaI ACNGT 1 cut(s) 1102
TaqI TCGA 3 cut(s) 17, 261, 283
TaqII GACCGA 1 cut(s) 1311
TasI AATT 9 cut(s) 62, 155, 305, 824, 856, 974, 1005, 1035, 1299
TatI WGTACW 2 cut(s) 645, 666
TauI GCSGC 1 cut(s) 754
TfiI GAWTC 5 cut(s) 7, 114, 466, 1186, 1225
Tru1I TTAA 8 cut(s) 60, 525, 591, 731, 954, 1017, 1119, 1154
Tru9I TTAA 8 cut(s) 60, 525, 591, 731, 954, 1017, 1119, 1154
TscAI CASTG 5 cut(s) 869, 884, 1006, 1240, 1458
TseI GCWGC 2 cut(s) 343, 1073
TspDTI ATGAA 9 cut(s) 109, 225, 261, 521, 686, 1155, 1217, 1267, 1357
TspGWI ACGGA 1 cut(s) 167
TspRI CASTG 5 cut(s) 869, 884, 1006, 1240, 1458
VpaK11BI GGWCC 1 cut(s) 1294
VspI ATTAAT 2 cut(s) 525, 591
XapI RAATTY 3 cut(s) 62, 1005, 1299
XceI RCATGY 2 cut(s) 230, 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 1240
XmiI GTMKAC 2 cut(s) 1195, 1440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.