Rorug03G0005800

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0012143
Physical Location & Seq
Reverse (-)
443919 .. 445597
1679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0005800.1

Sequence Viewer

Length: 1416 bp
ATGGAACCAGCTCCATTACTGGAAACACCAACTTTAGAGCCTTATGATGATGAAGAAGACGAAGACAAGTATCTGATACCAAAGCCATCAGAGTGGTTCCCAAAGCTAGTGTCTTTACAAGCTGATTTGATTTACAACTCCATCCTTGTTTTATGTCACCCCATACTCTCCATCTTCTCTTTCGCCTACGAGTCTTACCACCAAGTCGAGCAGGCCAAGGAGACGGTCGAGTCCGCCGTCCAAAAAGCCCCCTCCACCATCACACACGGCGCAGTCGTCCTCATCAAGAAGCTCGGCTTTGGAGTTCTCGGGGCCGCCTATGTCTGCATGGTGTTGTTTATGCTTTTGATTCTGGCCACAATTGTGGGAGTTGCTTTGGTGCGGCTCTGGCTGGAAGAGCCTGTGGTTGTTAGGGAGAGTTTGTACTTTGATTACACCCTAGCTCATCCCAAATCGGTGTTTGTGTTCGGGGGATCAGGGCTAGCTGCTGGGAGTGGTGCTGACTACTTCAAGATTATTAGCAGCAACAGTAAGCGTATGGCTATGGGGGTTCCTGTTGGACACACTTTTACTGTGTCTCTGGAGCTTTTGATGCCGGAGTCTGATTTTAATAGGCACATTGGGGTGTTTCAGATGACTGCAGAAGTATTATCAGCTAATGGTGATGTGATTGCCAAGTCAAGCCAGCCATGTATGCTACGATTTAGAAGCCTCCCAATTCGACTCACAAGAACATTTCTTATGGGTGTACCTCTGATACTGGGCATTTCTGGAGAAACCCAGAAGATAGCAGTACAAATACTGAGGCACAAGGAAGGGACTTATCCAAGAACCCGAGCAATAAGAATAACATTAAGTCCAAGAGCTGGTACCTCACATCTTCCACAGTTGTATGAGGCTGAAATTGTTTTGAATTCACAGCTGCCTTGGACAAAGCAATTGGTGCGCAGTTGGAAATGGACGCTCTATGTCTGGGCCTCTTTGTATTGTTACACTATATTCGTAATAACTTATGTAACGTGTTGTAGACCACTCTTGTCACCAGTGACAATGATCAAAACTGCGGCGAATTATGTTGCTAATCGTGTTAATCATGACCGATCAACTACTGATCATGTTCAAAGAGATAGAGGGGCGAGTACTGAAGTAAGACGGCGAAGACCCCTTCGAGTGCCTAAAGAGGCCACAACGACACTTAAAGATGATTCTGATCAGAGTGAGGATATTTCAGAGTTGTTGAGAAAGTGGCAGCGGAGCAGAAGCAAAAGAAAGGCAATCTATTTGCAAACGGATTTACCAAGTGAGACTGTTGGCACCTCATCTGCACCATCAACGATTAGTATGAGTACTCGAGAAGATGCAAGTACTGTAACTGCGGAAGAAGATGTTGGGGACTCGGAATCAGTGTGTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

471

Amino Acids

52.65

Weight (kDa)

7.14

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Seipin PF06775 113 - 329 1.8e-48 Putative adipose-regulatory protein (Seipin)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05390
fragaria_vesca FvH4_6g06250 FvH4_6g06250
malus_domestica MD12G1217000.v1.1
prunus_persica Prupe.6G323300_v2.0.a1
pyrus_communis pycom04g17960
rosa_chinensis RchiOBHm_Chr3g0455271
rosa_laevigata RLG00000025373
rosa_multiflora Rmu_sc0002169.1_g000037
rosa_roxburghii Rroxscaffold_6G00424410
rosa_rugosa Rorug03G0005500 Rorug03G0005600 Rorug03G0005700 Rorug03G0005800
rosa_samantha Rh3AG065600 Rh3BG067800 Rh3CG066200 Rh3DG067800
rosa_wichuraiana Rw3G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 229
Acc16I TGCGCA 1 cut(s) 947
Acc65I GGTACC 1 cut(s) 869
AccB1I GGYRCC 2 cut(s) 869, 1313
AccB7I CCANNNNNTGG 1 cut(s) 866
AccI GTMKAC 1 cut(s) 1027
AciI CCGC 6 cut(s) 234, 315, 382, 1064, 1252, 1376
AclWI GGATC 1 cut(s) 481
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 1 cut(s) 913
AcuI CTGAAG 1 cut(s) 1164
AfaI GTAC 8 cut(s) 425, 750, 795, 871, 1141, 1348, 1366, 1411
AfiI CCNNNNNNNGG 1 cut(s) 866
AflIII ACRYGT 1 cut(s) 1019
AgsI TTSAA 3 cut(s) 511, 913, 1121
AjuI GAANNNNNNNTTGG 2 cut(s) 1371, 1403
AleI CACNNNNGTG 1 cut(s) 362
Alw26I GTCTC 3 cut(s) 215, 582, 1298
AlwI GGATC 1 cut(s) 481
Ama87I CYCGRG 3 cut(s) 308, 834, 1350
AoxI GGCC 5 cut(s) 213, 312, 354, 975, 1182
ApeKI GCWGC 4 cut(s) 485, 522, 922, 1249
ApoI RAATTY 1 cut(s) 913
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
Asp718I GGTACC 1 cut(s) 869
AspLEI GCGC 2 cut(s) 272, 948
AspS9I GGNCC 2 cut(s) 312, 975
AsuHPI GGTGA 3 cut(s) 149, 674, 1032
AsuNHI GCTAGC 1 cut(s) 481
AvaI CYCGRG 3 cut(s) 308, 834, 1350
BalI TGGCCA 1 cut(s) 356
BanI GGYRCC 2 cut(s) 869, 1313
BbsI GAAGAC 3 cut(s) 63, 69, 1165
BbvI GCAGC 4 cut(s) 472, 534, 909, 1261
BccI CCATC 5 cut(s) 94, 149, 179, 266, 1336
BceAI ACGGC 3 cut(s) 221, 283, 1169
BclI TGATCA 3 cut(s) 1053, 1111, 1210
BcoDI GTCTC 3 cut(s) 215, 582, 1298
BfaI CTAG 3 cut(s) 107, 440, 482
BfmI CTRYAG 1 cut(s) 639
BisI GCNGC 7 cut(s) 315, 383, 486, 523, 923, 1065, 1250
BlsI GCNGC 7 cut(s) 316, 384, 487, 524, 924, 1066, 1251
BmcAI AGTACT 3 cut(s) 1141, 1348, 1366
BmeT110I CYCGRG 3 cut(s) 308, 834, 1350
BmgT120I GGNCC 2 cut(s) 312, 975
BmiI GGNNCC 6 cut(s) 6, 98, 313, 552, 871, 1315
BmrI ACTGGG 1 cut(s) 770
BmsI GCATC 2 cut(s) 582, 1348
BmtI GCTAGC 1 cut(s) 485
BmuI ACTGGG 1 cut(s) 770
BpiI GAAGAC 3 cut(s) 63, 69, 1165
BpmI CTGGAG 2 cut(s) 602, 792
BsaBI GATNNNNATC 1 cut(s) 1209
BsaJI CCNNGG 2 cut(s) 216, 926
BsaXI ACNNNNNCTCC 2 cut(s) 407, 437
Bsc4I CCNNNNNNNGG 1 cut(s) 866
Bse1I ACTGG 3 cut(s) 24, 765, 1043
Bse8I GATNNNNATC 1 cut(s) 1209
BseDI CCNNGG 2 cut(s) 216, 926
BseGI GGATG 2 cut(s) 141, 445
BseJI GATNNNNATC 1 cut(s) 1209
BseLI CCNNNNNNNGG 1 cut(s) 866
BseMII CTCAG 1 cut(s) 794
BseNI ACTGG 3 cut(s) 24, 765, 1043
BseXI GCAGC 4 cut(s) 472, 534, 909, 1261
BseYI CCCAGC 1 cut(s) 488
BsgI GTGCAG 1 cut(s) 1308
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 5 cut(s) 215, 314, 356, 977, 1184
BshNI GGYRCC 2 cut(s) 869, 1313
BsiEI CGRYCG 1 cut(s) 228
BsiHKCI CYCGRG 3 cut(s) 308, 834, 1350
BsiSI CCGG 1 cut(s) 596
BslFI GGGAC 2 cut(s) 832, 1406
BslI CCNNNNNNNGG 1 cut(s) 866
BsmAI GTCTC 3 cut(s) 215, 582, 1298
BsmBI CGTCTC 1 cut(s) 215
BsmFI GGGAC 2 cut(s) 832, 1406
BsnI GGCC 5 cut(s) 215, 314, 356, 977, 1184
BsoBI CYCGRG 3 cut(s) 308, 834, 1350
Bsp143I GATC 5 cut(s) 473, 1053, 1100, 1111, 1210
BspACI CCGC 6 cut(s) 234, 315, 382, 1064, 1252, 1376
BspANI GGCC 5 cut(s) 215, 314, 356, 977, 1184
BspCNI CTCAG 1 cut(s) 795
BspHI TCATGA 1 cut(s) 1093
BspLI GGNNCC 6 cut(s) 6, 98, 313, 552, 871, 1315
BspMAI CTGCAG 1 cut(s) 643
BspOI GCTAGC 1 cut(s) 485
BspPI GGATC 1 cut(s) 481
BspQI GCTCTTC 1 cut(s) 390
BspT107I GGYRCC 2 cut(s) 869, 1313
BsrI ACTGG 3 cut(s) 24, 765, 1043
BssECI CCNNGG 2 cut(s) 216, 926
BssMI GATC 5 cut(s) 473, 1053, 1100, 1111, 1210
BssT1I CCWWGG 2 cut(s) 216, 926
Bst4CI ACNGT 6 cut(s) 226, 530, 574, 888, 1309, 1369
Bst6I CTCTTC 1 cut(s) 390
BstAPI GCANNNNNTGC 1 cut(s) 943
BstC8I GCNNGC 3 cut(s) 213, 483, 686
BstDEI CTNAG 2 cut(s) 803, 1413
BstF5I GGATG 2 cut(s) 141, 445
BstHHI GCGC 2 cut(s) 272, 948
BstKTI GATC 5 cut(s) 476, 1056, 1103, 1114, 1213
BstMAI GTCTC 3 cut(s) 215, 582, 1298
BstMBI GATC 5 cut(s) 473, 1053, 1100, 1111, 1210
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 5 cut(s) 388, 397, 592, 694, 943
BstSFI CTRYAG 1 cut(s) 639
BstV1I GCAGC 4 cut(s) 472, 534, 909, 1261
BstV2I GAAGAC 3 cut(s) 63, 69, 1165
BstXI CCANNNNNNTGG 2 cut(s) 93, 364
BsuRI GGCC 5 cut(s) 215, 314, 356, 977, 1184
BtsCI GGATG 2 cut(s) 141, 445
BtsIMutI CAGTG 2 cut(s) 1050, 1410
Cac8I GCNNGC 3 cut(s) 213, 483, 686
CciI TCATGA 1 cut(s) 1093
CfoI GCGC 2 cut(s) 272, 948
Cfr13I GGNCC 2 cut(s) 312, 975
CseI GACGC 1 cut(s) 970
Csp6I GTAC 8 cut(s) 424, 749, 794, 870, 1140, 1347, 1365, 1410
CviAII CATG 4 cut(s) 328, 690, 1094, 1115
CviQI GTAC 8 cut(s) 424, 749, 794, 870, 1140, 1347, 1365, 1410
DdeI CTNAG 2 cut(s) 803, 1413
DpnI GATC 5 cut(s) 475, 1055, 1102, 1113, 1212
DpnII GATC 5 cut(s) 473, 1053, 1100, 1111, 1210
DrdI GACNNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
EciI GGCGGA 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 216, 926
Eco57I CTGAAG 1 cut(s) 1164
Eco88I CYCGRG 3 cut(s) 308, 834, 1350
EcoRI GAATTC 1 cut(s) 913
EcoT14I CCWWGG 2 cut(s) 216, 926
ErhI CCWWGG 2 cut(s) 216, 926
Esp3I CGTCTC 1 cut(s) 215
FaeI CATG 4 cut(s) 331, 693, 1097, 1118
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 2 cut(s) 832, 1406
FatI CATG 4 cut(s) 327, 689, 1093, 1114
FbaI TGATCA 3 cut(s) 1053, 1111, 1210
FblI GTMKAC 1 cut(s) 1027
Fnu4HI GCNGC 7 cut(s) 315, 383, 486, 523, 923, 1065, 1250
FokI GGATG 2 cut(s) 128, 432
Fsp4HI GCNGC 7 cut(s) 315, 383, 486, 523, 923, 1065, 1250
FspBI CTAG 3 cut(s) 107, 440, 482
FspI TGCGCA 1 cut(s) 947
GlaI GCGC 2 cut(s) 271, 947
GluI GCNGC 7 cut(s) 315, 383, 486, 523, 923, 1065, 1250
GsaI CCCAGC 1 cut(s) 492
GsuI CTGGAG 2 cut(s) 602, 792
HaeIII GGCC 5 cut(s) 215, 314, 356, 977, 1184
HapII CCGG 1 cut(s) 596
HgaI GACGC 1 cut(s) 970
HhaI GCGC 2 cut(s) 272, 948
Hin1II CATG 4 cut(s) 331, 693, 1097, 1118
Hin6I GCGC 2 cut(s) 270, 946
HinP1I GCGC 2 cut(s) 270, 946
HinfI GANTC 8 cut(s) 191, 230, 349, 599, 723, 1205, 1394, 1400
HpaII CCGG 1 cut(s) 596
HphI GGTGA 3 cut(s) 149, 674, 1032
Hpy166II GTNNAC 3 cut(s) 749, 1028, 1410
Hpy188I TCNGA 9 cut(s) 75, 91, 604, 633, 756, 1210, 1215, 1231, 1399
Hpy188III TCNNGA 6 cut(s) 286, 511, 581, 771, 1094, 1352
Hpy8I GTNNAC 3 cut(s) 749, 1028, 1410
HpyAV CCTTC 2 cut(s) 809, 1175
HpyCH4III ACNGT 6 cut(s) 226, 530, 574, 888, 1309, 1369
HpyCH4IV ACGT 1 cut(s) 1019
HpyCH4V TGCA 5 cut(s) 327, 641, 1285, 1325, 1361
HpyF10VI GCNNNNNNNGC 5 cut(s) 388, 397, 592, 694, 943
HpyF3I CTNAG 2 cut(s) 803, 1413
HpySE526I ACGT 1 cut(s) 1019
Hsp92II CATG 4 cut(s) 331, 693, 1097, 1118
HspAI GCGC 2 cut(s) 270, 946
KpnI GGTACC 1 cut(s) 873
Ksp22I TGATCA 3 cut(s) 1053, 1111, 1210
Kzo9I GATC 5 cut(s) 473, 1053, 1100, 1111, 1210
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 3 cut(s) 16, 583, 1254
Lsp1109I GCAGC 4 cut(s) 472, 534, 909, 1261
LweI GCATC 2 cut(s) 582, 1348
MaeI CTAG 3 cut(s) 107, 440, 482
MaeII ACGT 1 cut(s) 1019
MaeIII GTNAC 6 cut(s) 155, 989, 1015, 1038, 1045, 1369
MalI GATC 5 cut(s) 475, 1055, 1102, 1113, 1212
MboI GATC 5 cut(s) 473, 1053, 1100, 1111, 1210
MfeI CAATTG 2 cut(s) 360, 938
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 6 cut(s) 360, 717, 903, 913, 938, 1069
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 5 cut(s) 200, 239, 608, 717, 1388
MmeI TCCRAC 2 cut(s) 538, 932
Mox20I TGGCCA 1 cut(s) 356
MscI TGGCCA 1 cut(s) 356
MseI TTAA 4 cut(s) 609, 854, 1089, 1197
MslI CAYNNNNRTG 3 cut(s) 91, 362, 623
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 2 cut(s) 922, 1252
MspI CCGG 1 cut(s) 596
MunI CAATTG 2 cut(s) 360, 938
MwoI GCNNNNNNNGC 5 cut(s) 388, 397, 592, 694, 943
NdeII GATC 5 cut(s) 473, 1053, 1100, 1111, 1210
NheI GCTAGC 1 cut(s) 481
NlaIII CATG 4 cut(s) 331, 693, 1097, 1118
NlaIV GGNNCC 6 cut(s) 6, 98, 313, 552, 871, 1315
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 3 cut(s) 155, 1038, 1045
NsbI TGCGCA 1 cut(s) 947
OliI CACNNNNGTG 1 cut(s) 362
PaeR7I CTCGAG 1 cut(s) 1350
PagI TCATGA 1 cut(s) 1093
PciSI GCTCTTC 1 cut(s) 390
PcsI WCGNNNNNNNCGW 2 cut(s) 234, 273
PfeI GAWTC 3 cut(s) 349, 1205, 1400
PflMI CCANNNNNTGG 1 cut(s) 866
PkrI GCNGC 7 cut(s) 316, 384, 487, 524, 924, 1066, 1251
PleI GAGTC 5 cut(s) 199, 238, 607, 717, 1388
PpsI GAGTC 5 cut(s) 199, 238, 607, 717, 1388
PspFI CCCAGC 1 cut(s) 488
PspN4I GGNNCC 6 cut(s) 6, 98, 313, 552, 871, 1315
PspPI GGNCC 2 cut(s) 312, 975
PstI CTGCAG 1 cut(s) 643
PvuII CAGCTG 1 cut(s) 922
RsaI GTAC 8 cut(s) 425, 750, 795, 871, 1141, 1348, 1366, 1411
RsaNI GTAC 8 cut(s) 424, 749, 794, 870, 1140, 1347, 1365, 1410
RseI CAYNNNNRTG 3 cut(s) 91, 362, 623
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 4 cut(s) 609, 854, 1089, 1197
SatI GCNGC 7 cut(s) 315, 383, 486, 523, 923, 1065, 1250
Sau3AI GATC 5 cut(s) 473, 1053, 1100, 1111, 1210
Sau96I GGNCC 2 cut(s) 312, 975
ScaI AGTACT 3 cut(s) 1141, 1348, 1366
SchI GAGTC 5 cut(s) 200, 239, 608, 717, 1388
SfaNI GCATC 2 cut(s) 582, 1348
SfcI CTRYAG 1 cut(s) 639
Sfr274I CTCGAG 1 cut(s) 1350
SlaI CTCGAG 1 cut(s) 1350
SmiMI CAYNNNNRTG 3 cut(s) 91, 362, 623
SmlI CTYRAG 1 cut(s) 1350
SmoI CTYRAG 1 cut(s) 1350
Sse9I AATT 6 cut(s) 360, 717, 903, 913, 938, 1069
SsiI CCGC 6 cut(s) 234, 315, 382, 1064, 1252, 1376
SspMI CTAG 3 cut(s) 107, 440, 482
StyI CCWWGG 2 cut(s) 216, 926
TaaI ACNGT 6 cut(s) 226, 530, 574, 888, 1309, 1369
TaiI ACGT 1 cut(s) 1022
TaqI TCGA 5 cut(s) 207, 228, 721, 1168, 1351
TaqII GACCGA 1 cut(s) 1113
TasI AATT 6 cut(s) 360, 717, 903, 913, 938, 1069
TatI WGTACW 6 cut(s) 423, 793, 1139, 1346, 1364, 1409
TauI GCSGC 3 cut(s) 317, 385, 1067
TfiI GAWTC 3 cut(s) 349, 1205, 1400
Tru1I TTAA 4 cut(s) 609, 854, 1089, 1197
Tru9I TTAA 4 cut(s) 609, 854, 1089, 1197
TscAI CASTG 2 cut(s) 1050, 1410
TseFI GTSAC 3 cut(s) 155, 1038, 1045
TseI GCWGC 4 cut(s) 485, 522, 922, 1249
Tsp45I GTSAC 3 cut(s) 155, 1038, 1045
TspDTI ATGAA 1 cut(s) 66
TspGWI ACGGA 1 cut(s) 1304
TspRI CASTG 2 cut(s) 1050, 1410
Van91I CCANNNNNTGG 1 cut(s) 866
XapI RAATTY 1 cut(s) 913
XhoI CTCGAG 1 cut(s) 1350
XmiI GTMKAC 1 cut(s) 1027
XspI CTAG 3 cut(s) 107, 440, 482
ZrmI AGTACT 3 cut(s) 1141, 1348, 1366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.