Rroxscaffold_6G00424410

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012143
Physical Location & Seq
Forward (+)
45556303 .. 45559472
3170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00424410.1

Sequence Viewer

Length: 1410 bp
ATGAGGAGGTCATGCCTTCCCAAATGCAGATTTGGTAGGATAATGGGATGGCTCCAGATCTTATTGGGAGGGCTTGTTATAATTGTCAGCATATCCAGCCTCTTCAAGTTCTACTCTGCTGGATTTTTCTTGCGCAATGAAGACATATGCCGACATGTTTATGGGGTCAGAGATGAATTTGGGGGCTTTGACATAAAAGCTCTGTCTCATCGAGTAGGGGAAGTGCTAAACAAATTGGATAGTTTACAAGAAAAGCTTGAGTCAGCTGTGCAGCAAATGGAGAAGAACAAAGATGTCTTGCATACAACTAATATCACAAAACTGGAGTTCAAGAAGTATCTGAAGGAGCAAGTAATCAGGCCTCTTTATAGTGCTCACATTTCTCTGAGACTGATTCGGTTACCTAACCCAGAAGGTATTAGAAACTCTACAATGAAAGAGGAACCTTTGATTAATACTTTTGTGATTGAAGAGATAAGGAAGTATATAACCCCAAAGGAGAGTAGAATTGGGAAGATTAATATATATGGTACAGAGAGGATATACAATACGATTGGACATGCTTGTGTGTTGTACAAGAAAGCGTTGGAAGAGTACATGAACTATGACATCGGATCTTATTGTAACGATGATTGGAACTTGGCTCAGAAGCTGATGTTAAATGGTTGTGATCCTTTGCCGCGGAGGCGATGTTTGACAAGGGCCTCAAAGGTCTATCAGAAGCCTTATCCGATCAATGAGTCTCTGTGGAAATTGCCGGATGACAGAAATGTGCGGTGGAGCAATTATCAGTGCAGGAACTTTCAGTGCTTGATGAGCAATAACTCCAAGAGGGGCTATTCAAAGTGCGTAGGATGTTTTGAAATGGAGAAGGAAAAGCTTAAATGGATGATTACCAATAAATCACTACCAGTTGATTTCCTGATCAGTGAAATTTTGGCCATTAAGCCTGGGGAGATAAGAATTGGTCTTGACTTTGGTGTTGGCACAGGAACTTATGCTGCAAGAATGAGAGAAAATAATGTTACAGTAGTCTCTACTGCTCTTAACCTTGGGGCTCCTTTCAATGAAATGATTGCATTAAGAGGTTTGGTTCCGCTGTATGTAACATTGAATCAGCGTCTACCTTTCTTTGACAATATAATGGACTTGATTCATACCGCTGGGTTTCTGGATGGCTGGATTGACCTGCAGTTGCTGGATTTCATCCTGTTTGATTGGGACCGAATTCTGAGGCCCGGCGGATTGTTATGGATTGACCGGTTCTTCTGCAATAAAAAGGATTTGGATGATTTCATGTATATGTTTCTGCAGTTGAGATACAAGAAACACAAGTGGGTTATTTCTCCCAAATCAAAGGATGAGGTCTACTTTTCTGCAGTGTTAGAGAAACCTCCAAGAGCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

469

Amino Acids

54.72

Weight (kDa)

9.24

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 312 - 418 2.9e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05390
fragaria_vesca FvH4_6g06250 FvH4_6g06250
malus_domestica MD12G1217000.v1.1
prunus_persica Prupe.6G323300_v2.0.a1
pyrus_communis pycom04g17960
rosa_chinensis RchiOBHm_Chr3g0455271
rosa_laevigata RLG00000025373
rosa_multiflora Rmu_sc0002169.1_g000037
rosa_roxburghii Rroxscaffold_6G00424410
rosa_rugosa Rorug03G0005500 Rorug03G0005600 Rorug03G0005700 Rorug03G0005800
rosa_samantha Rh3AG065600 Rh3BG067800 Rh3CG066200 Rh3DG067800
rosa_wichuraiana Rw3G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 80
Acc16I TGCGCA 1 cut(s) 134
Acc36I ACCTGC 1 cut(s) 1197
AccI GTMKAC 2 cut(s) 1123, 1368
AccII CGCG 1 cut(s) 682
AciI CCGC 6 cut(s) 680, 682, 775, 1097, 1161, 1242
AclWI GGATC 2 cut(s) 622, 665
AcoI YGGCCR 1 cut(s) 939
AcsI RAATTY 3 cut(s) 176, 933, 1227
AcuI CTGAAG 1 cut(s) 362
AfaI GTAC 3 cut(s) 532, 575, 596
AflIII ACRYGT 1 cut(s) 154
AgeI ACCGGT 1 cut(s) 1260
AgsI TTSAA 7 cut(s) 106, 331, 470, 843, 863, 1066, 1114
AjnI CCWGG 1 cut(s) 949
AluBI AGCT 5 cut(s) 200, 256, 266, 652, 880
AluI AGCT 5 cut(s) 200, 256, 266, 652, 880
Alw21I GWGCWC 1 cut(s) 376
Alw26I GTCTC 4 cut(s) 210, 382, 747, 1039
AlwI GGATC 2 cut(s) 622, 665
AlwNI CAGNNNCTG 2 cut(s) 652, 1198
AoxI GGCC 4 cut(s) 359, 702, 939, 1235
ApeKI GCWGC 2 cut(s) 271, 1001
ApoI RAATTY 3 cut(s) 176, 933, 1227
AseI ATTAAT 2 cut(s) 453, 519
AsiGI ACCGGT 1 cut(s) 1260
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 3 cut(s) 702, 1222, 1236
AsuC2I CCSGG 1 cut(s) 1239
AvaII GGWCC 1 cut(s) 1222
BalI TGGCCA 1 cut(s) 941
BanII GRGCYC 1 cut(s) 1060
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 376
BbvI GCAGC 2 cut(s) 283, 988
BccI CCATC 2 cut(s) 42, 1169
BciT130I CCWGG 1 cut(s) 951
BclI TGATCA 1 cut(s) 924
BcnI CCSGG 1 cut(s) 1239
BcoDI GTCTC 4 cut(s) 210, 382, 747, 1039
BfmI CTRYAG 3 cut(s) 1190, 1310, 1377
BfuAI ACCTGC 1 cut(s) 1197
BglI GCCNNNNNGGC 1 cut(s) 685
BglII AGATCT 1 cut(s) 57
BisI GCNGC 3 cut(s) 272, 680, 1002
BlsI GCNGC 3 cut(s) 273, 681, 1003
Bme1390I CCNGG 2 cut(s) 951, 1239
Bme18I GGWCC 1 cut(s) 1222
BmgT120I GGNCC 3 cut(s) 702, 1222, 1236
BmiI GGNNCC 5 cut(s) 53, 444, 1059, 1095, 1223
BmrFI CCNGG 2 cut(s) 951, 1239
BpiI GAAGAC 1 cut(s) 147
BpmI CTGGAG 2 cut(s) 38, 344
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 1239
BsaJI CCNNGG 3 cut(s) 680, 950, 1051
BsaWI WCCGGW 1 cut(s) 1260
BsaXI ACNNNNNCTCC 4 cut(s) 60, 90, 676, 706
Bse118I RCCGGY 1 cut(s) 1260
Bse1I ACTGG 2 cut(s) 327, 911
Bse3DI GCAATG 1 cut(s) 142
BseBI CCWGG 1 cut(s) 951
BseDI CCNNGG 3 cut(s) 680, 950, 1051
BseGI GGATG 8 cut(s) 53, 766, 860, 894, 1180, 1206, 1294, 1366
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 3 cut(s) 377, 659, 1223
BseNI ACTGG 2 cut(s) 327, 911
BseRI GAGGAG 1 cut(s) 19
BseXI GCAGC 2 cut(s) 283, 988
BseYI CCCAGC 1 cut(s) 1163
BsgI GTGCAG 2 cut(s) 290, 814
Bsh1236I CGCG 1 cut(s) 682
BshFI GGCC 4 cut(s) 361, 704, 941, 1237
BshTI ACCGGT 1 cut(s) 1260
BsiHKAI GWGCWC 1 cut(s) 376
BsiSI CCGG 3 cut(s) 758, 1239, 1261
BslFI GGGAC 1 cut(s) 1235
BsmAI GTCTC 4 cut(s) 210, 382, 747, 1039
BsmFI GGGAC 1 cut(s) 1235
BsnI GGCC 4 cut(s) 361, 704, 941, 1237
Bsp1286I GDGCHC 2 cut(s) 376, 1060
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 5 cut(s) 57, 614, 670, 732, 924
BspACI CCGC 6 cut(s) 680, 682, 775, 1097, 1161, 1242
BspANI GGCC 4 cut(s) 361, 704, 941, 1237
BspCNI CTCAG 3 cut(s) 378, 658, 1224
BspFNI CGCG 1 cut(s) 682
BspLI GGNNCC 5 cut(s) 53, 444, 1059, 1095, 1223
BspMAI CTGCAG 3 cut(s) 1194, 1314, 1381
BspMI ACCTGC 1 cut(s) 1197
BspPI GGATC 2 cut(s) 622, 665
BsrDI GCAATG 1 cut(s) 142
BsrFI RCCGGY 1 cut(s) 1260
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 2 cut(s) 327, 911
BssAI RCCGGY 1 cut(s) 1260
BssECI CCNNGG 3 cut(s) 680, 950, 1051
BssMI GATC 5 cut(s) 57, 614, 670, 732, 924
BssT1I CCWWGG 1 cut(s) 1051
Bst2UI CCWGG 1 cut(s) 951
Bst4CI ACNGT 1 cut(s) 1030
Bst6I CTCTTC 3 cut(s) 107, 465, 585
BstAUI TGTACA 1 cut(s) 573
BstDEI CTNAG 3 cut(s) 386, 645, 1232
BstDSI CCRYGG 1 cut(s) 680
BstEII GGTNACC 1 cut(s) 399
BstF5I GGATG 8 cut(s) 53, 766, 860, 894, 1180, 1206, 1294, 1366
BstFNI CGCG 1 cut(s) 682
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 5 cut(s) 60, 617, 673, 735, 927
BstMAI GTCTC 4 cut(s) 210, 382, 747, 1039
BstMBI GATC 5 cut(s) 57, 614, 670, 732, 924
BstMWI GCNNNNNNNGC 3 cut(s) 96, 685, 816
BstNI CCWGG 1 cut(s) 951
BstNSI RCATGY 2 cut(s) 158, 563
BstPI GGTNACC 1 cut(s) 399
BstSCI CCNGG 2 cut(s) 949, 1237
BstSFI CTRYAG 3 cut(s) 1190, 1310, 1377
BstUI CGCG 1 cut(s) 682
BstV1I GCAGC 2 cut(s) 283, 988
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 2 cut(s) 57, 614
BstYI RGATCY 2 cut(s) 57, 614
BsuRI GGCC 4 cut(s) 361, 704, 941, 1237
BtgI CCRYGG 1 cut(s) 680
BtgZI GCGATG 1 cut(s) 703
BtsCI GGATG 8 cut(s) 53, 766, 860, 894, 1180, 1206, 1294, 1366
BtsI GCAGTG 1 cut(s) 1386
BtsIMutI CAGTG 4 cut(s) 797, 812, 934, 1386
BveI ACCTGC 1 cut(s) 1197
CaiI CAGNNNCTG 2 cut(s) 652, 1198
CfoI GCGC 1 cut(s) 135
Cfr10I RCCGGY 1 cut(s) 1260
Cfr13I GGNCC 3 cut(s) 702, 1222, 1236
Cfr42I CCGCGG 1 cut(s) 683
CseI GACGC 1 cut(s) 1109
Csp6I GTAC 3 cut(s) 531, 574, 595
CspAI ACCGGT 1 cut(s) 1260
CviAII CATG 5 cut(s) 12, 155, 560, 598, 1297
CviQI GTAC 3 cut(s) 531, 574, 595
DdeI CTNAG 3 cut(s) 386, 645, 1232
DpnI GATC 5 cut(s) 59, 616, 672, 734, 926
DpnII GATC 5 cut(s) 57, 614, 670, 732, 924
EaeI YGGCCR 1 cut(s) 939
Eam1104I CTCTTC 3 cut(s) 107, 465, 585
EarI CTCTTC 3 cut(s) 107, 465, 585
EciI GGCGGA 1 cut(s) 1257
Eco130I CCWWGG 1 cut(s) 1051
Eco147I AGGCCT 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 1060
Eco47I GGWCC 1 cut(s) 1222
Eco57I CTGAAG 1 cut(s) 362
Eco91I GGTNACC 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 702
EcoO65I GGTNACC 1 cut(s) 399
EcoRI GAATTC 1 cut(s) 1227
EcoRII CCWGG 1 cut(s) 949
EcoT14I CCWWGG 1 cut(s) 1051
EcoT38I GRGCYC 1 cut(s) 1060
ErhI CCWWGG 1 cut(s) 1051
FaeI CATG 5 cut(s) 15, 158, 563, 601, 1300
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FaqI GGGAC 1 cut(s) 1235
FatI CATG 5 cut(s) 11, 154, 559, 597, 1296
FauNDI CATATG 1 cut(s) 146
FbaI TGATCA 1 cut(s) 924
FblI GTMKAC 2 cut(s) 1123, 1368
Fnu4HI GCNGC 3 cut(s) 272, 680, 1002
FokI GGATG 8 cut(s) 60, 773, 867, 901, 1187, 1193, 1301, 1373
FriOI GRGCYC 1 cut(s) 1060
Fsp4HI GCNGC 3 cut(s) 272, 680, 1002
FspI TGCGCA 1 cut(s) 134
GlaI GCGC 1 cut(s) 134
GluI GCNGC 3 cut(s) 272, 680, 1002
GsaI CCCAGC 1 cut(s) 1167
GsuI CTGGAG 2 cut(s) 38, 344
HaeIII GGCC 4 cut(s) 361, 704, 941, 1237
HapII CCGG 3 cut(s) 758, 1239, 1261
HgaI GACGC 1 cut(s) 1109
HhaI GCGC 1 cut(s) 135
Hin1II CATG 5 cut(s) 15, 158, 563, 601, 1300
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HindIII AAGCTT 2 cut(s) 254, 878
HinfI GANTC 5 cut(s) 260, 394, 740, 1114, 1153
HpaII CCGG 3 cut(s) 758, 1239, 1261
Hpy166II GTNNAC 3 cut(s) 245, 1124, 1369
Hpy188I TCNGA 9 cut(s) 170, 342, 387, 614, 648, 720, 732, 1233, 1409
Hpy188III TCNNGA 5 cut(s) 55, 331, 922, 971, 1172
Hpy8I GTNNAC 3 cut(s) 245, 1124, 1369
HpyAV CCTTC 4 cut(s) 26, 337, 407, 865
HpyCH4III ACNGT 1 cut(s) 1030
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 685, 816
HpyF3I CTNAG 3 cut(s) 386, 645, 1232
Hsp92II CATG 5 cut(s) 15, 158, 563, 601, 1300
HspAI GCGC 1 cut(s) 133
Ksp22I TGATCA 1 cut(s) 924
KspI CCGCGG 1 cut(s) 683
Kzo9I GATC 5 cut(s) 57, 614, 670, 732, 924
LmnI GCTCC 4 cut(s) 57, 346, 780, 1063
Lsp1109I GCAGC 2 cut(s) 283, 988
MaeIII GTNAC 4 cut(s) 399, 623, 1024, 1105
MalI GATC 5 cut(s) 59, 616, 672, 734, 926
MboI GATC 5 cut(s) 57, 614, 670, 732, 924
MboII GAAGA 7 cut(s) 94, 152, 295, 482, 526, 602, 1258
MflI RGATCY 2 cut(s) 57, 614
MhlI GDGCHC 2 cut(s) 376, 1060
MlsI TGGCCA 1 cut(s) 941
MluCI AATT 9 cut(s) 81, 176, 233, 507, 752, 784, 933, 963, 1227
MluNI TGGCCA 1 cut(s) 941
MlyI GAGTC 2 cut(s) 269, 749
MmeI TCCRAC 1 cut(s) 567
Mox20I TGGCCA 1 cut(s) 941
MscI TGGCCA 1 cut(s) 941
MseI TTAA 7 cut(s) 453, 519, 659, 882, 945, 1047, 1082
MslI CAYNNNNRTG 3 cut(s) 159, 564, 1301
Msp20I TGGCCA 1 cut(s) 941
MspA1I CMGCKG 4 cut(s) 266, 682, 1099, 1163
MspI CCGG 3 cut(s) 758, 1239, 1261
MspR9I CCNGG 2 cut(s) 951, 1239
MvaI CCWGG 1 cut(s) 951
MvnI CGCG 1 cut(s) 682
MwoI GCNNNNNNNGC 3 cut(s) 96, 685, 816
NciI CCSGG 1 cut(s) 1239
NdeI CATATG 1 cut(s) 146
NdeII GATC 5 cut(s) 57, 614, 670, 732, 924
NlaIII CATG 5 cut(s) 15, 158, 563, 601, 1300
NlaIV GGNNCC 5 cut(s) 53, 444, 1059, 1095, 1223
NsbI TGCGCA 1 cut(s) 134
NspI RCATGY 2 cut(s) 158, 563
PceI AGGCCT 1 cut(s) 361
PciI ACATGT 1 cut(s) 154
PfeI GAWTC 3 cut(s) 394, 1114, 1153
PinAI ACCGGT 1 cut(s) 1260
PkrI GCNGC 3 cut(s) 273, 681, 1003
PleI GAGTC 2 cut(s) 268, 748
PpsI GAGTC 2 cut(s) 268, 748
PscI ACATGT 1 cut(s) 154
PshBI ATTAAT 2 cut(s) 453, 519
PsiI TTATAA 1 cut(s) 80
Psp6I CCWGG 1 cut(s) 949
PspEI GGTNACC 1 cut(s) 399
PspFI CCCAGC 1 cut(s) 1163
PspGI CCWGG 1 cut(s) 949
PspN4I GGNNCC 5 cut(s) 53, 444, 1059, 1095, 1223
PspPI GGNCC 3 cut(s) 702, 1222, 1236
PstI CTGCAG 3 cut(s) 1194, 1314, 1381
PstNI CAGNNNCTG 2 cut(s) 652, 1198
PsuI RGATCY 2 cut(s) 57, 614
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 3 cut(s) 532, 575, 596
RsaNI GTAC 3 cut(s) 531, 574, 595
RseI CAYNNNNRTG 3 cut(s) 159, 564, 1301
SacII CCGCGG 1 cut(s) 683
SaqAI TTAA 7 cut(s) 453, 519, 659, 882, 945, 1047, 1082
SatI GCNGC 3 cut(s) 272, 680, 1002
Sau3AI GATC 5 cut(s) 57, 614, 670, 732, 924
Sau96I GGNCC 3 cut(s) 702, 1222, 1236
SchI GAGTC 2 cut(s) 269, 749
ScrFI CCNGG 2 cut(s) 951, 1239
SduI GDGCHC 2 cut(s) 376, 1060
SfcI CTRYAG 3 cut(s) 1190, 1310, 1377
Sfr303I CCGCGG 1 cut(s) 683
SgrBI CCGCGG 1 cut(s) 683
SinI GGWCC 1 cut(s) 1222
SmiMI CAYNNNNRTG 3 cut(s) 159, 564, 1301
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 9 cut(s) 81, 176, 233, 507, 752, 784, 933, 963, 1227
SseBI AGGCCT 1 cut(s) 361
SsiI CCGC 6 cut(s) 680, 682, 775, 1097, 1161, 1242
StuI AGGCCT 1 cut(s) 361
StyD4I CCNGG 2 cut(s) 949, 1237
StyI CCWWGG 1 cut(s) 1051
TaaI ACNGT 1 cut(s) 1030
TaqI TCGA 1 cut(s) 211
TaqII GACCGA 1 cut(s) 1239
TasI AATT 9 cut(s) 81, 176, 233, 507, 752, 784, 933, 963, 1227
TatI WGTACW 2 cut(s) 573, 594
TauI GCSGC 1 cut(s) 682
TfiI GAWTC 3 cut(s) 394, 1114, 1153
Tru1I TTAA 7 cut(s) 453, 519, 659, 882, 945, 1047, 1082
Tru9I TTAA 7 cut(s) 453, 519, 659, 882, 945, 1047, 1082
TscAI CASTG 4 cut(s) 797, 812, 934, 1386
TseI GCWGC 2 cut(s) 271, 1001
TspDTI ATGAA 8 cut(s) 153, 189, 449, 614, 1083, 1145, 1195, 1285
TspRI CASTG 4 cut(s) 797, 812, 934, 1386
VpaK11BI GGWCC 1 cut(s) 1222
VspI ATTAAT 2 cut(s) 453, 519
XapI RAATTY 3 cut(s) 176, 933, 1227
XceI RCATGY 2 cut(s) 158, 563
XmiI GTMKAC 2 cut(s) 1123, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.