AT3G05520

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008545
Physical Location & Seq
Forward (+)
1598449 .. 1601437
2989 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05520.2

Sequence Viewer

Length: 927 bp
ATGGCGGACGAAGAAGATGAGTTACTGGAGACAGAACTAAGTTACGACCAGAAAAAGGAAATCGCCAAGTGGTTTTTCCTCAACGCTCCCGCTGGAGAAATCAATTACGTCGCTAAAGATTTAAAGGCGGTTTTGAGCGATGAGGAAGTGTACAATGAAGCAGCCATGGAAGCATTTCCAGTGTACAACAAAACTCACATGATTTGCCTTGAAATGCCCAGTGGAGCTGGTGATGTGATTGTCTCATCTTACAGTGAAATTAATGAAAATGAGTACCTTGATCCAAGAACTGCCCAGGTTGCCATAGTTGATCATGTTAAACAGATTTGTACAAAGGTACGGCCTGCAAATGATGAAGAACTTCCTTCTTTGTATATTGAGGAGTACAGATATGCTCTTGATGCTGAAATCCAGAGATATGTTAGTGAATCTTATCCGAAAGGTATGTCTGCAGTTAATTGTGTGAAAGGGAAAGATAATGAGGGTCCAGGGTCCGACTTTGAGCTTGTTGTTATAATTACTGCTATGAGACTTAGTCCCCAGAACTTCTGTAATGGAAGTTGGAGATCTGTATGGAACATAGATTTCCAGGATGAATCCCAGGTGCTTGACATAAAAGGGAAATTGCAGGTAGGAGCTCATTATTTTGAAGAGGGAAATGTGGAATTAGATGCAAAAAAAGATTTCCAAGATTCAACAATATTTCAGTCTGCAGATGACTGCGCGATTGCCATAGCCAATATTATCAGGCACCATGAAACAGAGTACCTTGCATCTCTAGAGGTAGCTTATTCAAAATTACCGGATAACACTTTCAAGGATTTGAGGAGGAAACTTCCGGTGACACGAACTCTGTTCCCATGGCAGAACACTTTACAGTTCAGCCTAACAAGAGAGGTCGAGAAAGAACTTGGACTTGGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

308

Amino Acids

35.04

Weight (kDa)

4.58

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-actin_cap_A PF01267 22 - 298 3.2e-78 F-actin capping protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 515
Acc36I ACCTGC 1 cut(s) 619
AccB1I GGYRCC 1 cut(s) 750
AccII CGCG 1 cut(s) 725
AciI CCGC 3 cut(s) 5, 90, 128
AclWI GGATC 1 cut(s) 275
AfaI GTAC 7 cut(s) 152, 185, 275, 331, 339, 386, 767
AfiI CCNNNNNNNGG 1 cut(s) 55
AgsI TTSAA 5 cut(s) 212, 650, 696, 795, 817
AjnI CCWGG 4 cut(s) 294, 487, 588, 600
AjuI GAANNNNNNNTTGG 3 cut(s) 59, 91, 900
AluBI AGCT 4 cut(s) 227, 505, 638, 788
AluI AGCT 4 cut(s) 227, 505, 638, 788
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 3 cut(s) 23, 247, 523
AlwI GGATC 1 cut(s) 275
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 1 cut(s) 161
ArsI GACNNNNNNTTYG 2 cut(s) 431, 463
AseI ATTAAT 1 cut(s) 261
Asp700I GAANNNNTTC 2 cut(s) 174, 360
AspLEI GCGC 1 cut(s) 725
AspS9I GGNCC 2 cut(s) 485, 492
AsuHPI GGTGA 2 cut(s) 242, 853
AvaII GGWCC 2 cut(s) 485, 492
BanI GGYRCC 1 cut(s) 750
BanII GRGCYC 1 cut(s) 640
BarI GAAGNNNNNNTAC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 640
BbvI GCAGC 1 cut(s) 173
BceAI ACGGC 1 cut(s) 356
BciT130I CCWGG 4 cut(s) 296, 489, 590, 602
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 3 cut(s) 23, 247, 523
BfaI CTAG 1 cut(s) 779
BfmI CTRYAG 2 cut(s) 450, 711
BfuAI ACCTGC 1 cut(s) 619
BglII AGATCT 1 cut(s) 566
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 4 cut(s) 296, 489, 590, 602
Bme18I GGWCC 2 cut(s) 485, 492
BmgT120I GGNCC 2 cut(s) 485, 492
BmiI GGNNCC 3 cut(s) 486, 493, 752
BmrFI CCNGG 4 cut(s) 296, 489, 590, 602
BmrI ACTGGG 1 cut(s) 213
BmsI GCATC 3 cut(s) 391, 661, 782
BmuI ACTGGG 1 cut(s) 213
BpmI CTGGAG 2 cut(s) 47, 114
BsaJI CCNNGG 5 cut(s) 165, 294, 488, 600, 860
BsaWI WCCGGW 2 cut(s) 802, 838
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 30, 179, 219
BseBI CCWGG 4 cut(s) 296, 489, 590, 602
BseDI CCNNGG 5 cut(s) 165, 294, 488, 600, 860
BseGI GGATG 1 cut(s) 598
BseLI CCNNNNNNNGG 1 cut(s) 55
BseNI ACTGG 3 cut(s) 30, 179, 219
BseRI GAGGAG 2 cut(s) 395, 841
BseXI GCAGC 1 cut(s) 173
Bsh1236I CGCG 1 cut(s) 725
BshFI GGCC 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 750
BsiHKAI GWGCWC 1 cut(s) 640
BsiSI CCGG 2 cut(s) 803, 839
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 3 cut(s) 23, 247, 523
BsmFI GGGAC 1 cut(s) 522
BsnI GGCC 1 cut(s) 343
Bsp1286I GDGCHC 1 cut(s) 640
Bsp1407I TGTACA 3 cut(s) 150, 183, 329
Bsp143I GATC 3 cut(s) 280, 310, 566
Bsp19I CCATGG 2 cut(s) 165, 860
BspACI CCGC 3 cut(s) 5, 90, 128
BspANI GGCC 1 cut(s) 343
BspFNI CGCG 1 cut(s) 725
BspLI GGNNCC 3 cut(s) 486, 493, 752
BspMAI CTGCAG 2 cut(s) 454, 715
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 1 cut(s) 275
BspT107I GGYRCC 1 cut(s) 750
BsrGI TGTACA 3 cut(s) 150, 183, 329
BsrI ACTGG 3 cut(s) 30, 179, 219
BssECI CCNNGG 5 cut(s) 165, 294, 488, 600, 860
BssMI GATC 3 cut(s) 280, 310, 566
BssT1I CCWWGG 2 cut(s) 165, 860
Bst2UI CCWGG 4 cut(s) 296, 489, 590, 602
Bst4CI ACNGT 2 cut(s) 254, 879
Bst6I CTCTTC 1 cut(s) 645
BstAUI TGTACA 3 cut(s) 150, 183, 329
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 2 cut(s) 38, 533
BstDSI CCRYGG 2 cut(s) 165, 860
BstF5I GGATG 1 cut(s) 598
BstFNI CGCG 1 cut(s) 725
BstHHI GCGC 1 cut(s) 725
BstKTI GATC 3 cut(s) 283, 313, 569
BstMAI GTCTC 3 cut(s) 23, 247, 523
BstMBI GATC 3 cut(s) 280, 310, 566
BstMWI GCNNNNNNNGC 3 cut(s) 170, 299, 401
BstNI CCWGG 4 cut(s) 296, 489, 590, 602
BstSCI CCNGG 4 cut(s) 294, 487, 588, 600
BstSFI CTRYAG 2 cut(s) 450, 711
BstUI CGCG 1 cut(s) 725
BstV1I GCAGC 1 cut(s) 173
BstX2I RGATCY 1 cut(s) 566
BstYI RGATCY 1 cut(s) 566
BsuRI GGCC 1 cut(s) 343
BtgI CCRYGG 2 cut(s) 165, 860
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 598
BtsIMutI CAGTG 3 cut(s) 186, 226, 259
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 1 cut(s) 345
CfoI GCGC 1 cut(s) 725
Cfr13I GGNCC 2 cut(s) 485, 492
Csp6I GTAC 7 cut(s) 151, 184, 274, 330, 338, 385, 766
CviAII CATG 5 cut(s) 166, 199, 314, 755, 861
CviJI RGCY 8 cut(s) 164, 227, 343, 505, 638, 737, 788, 885
CviKI_1 RGCY 8 cut(s) 164, 227, 343, 505, 638, 737, 788, 885
CviQI GTAC 7 cut(s) 151, 184, 274, 330, 338, 385, 766
DdeI CTNAG 2 cut(s) 38, 533
DpnI GATC 3 cut(s) 282, 312, 568
DpnII GATC 3 cut(s) 280, 310, 566
DraI TTTAAA 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 645
EarI CTCTTC 1 cut(s) 645
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 638
Eco130I CCWWGG 2 cut(s) 165, 860
Eco24I GRGCYC 1 cut(s) 640
Eco47I GGWCC 2 cut(s) 485, 492
Eco53kI GAGCTC 1 cut(s) 638
EcoICRI GAGCTC 1 cut(s) 638
EcoRII CCWGG 4 cut(s) 294, 487, 588, 600
EcoT14I CCWWGG 2 cut(s) 165, 860
EcoT38I GRGCYC 1 cut(s) 640
ErhI CCWWGG 2 cut(s) 165, 860
FaeI CATG 5 cut(s) 169, 202, 317, 758, 864
FaqI GGGAC 1 cut(s) 522
FatI CATG 5 cut(s) 165, 198, 313, 754, 860
FauI CCCGC 1 cut(s) 97
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 1 cut(s) 605
FriOI GRGCYC 1 cut(s) 640
Fsp4HI GCNGC 1 cut(s) 162
FspBI CTAG 1 cut(s) 779
GlaI GCGC 1 cut(s) 724
GluI GCNGC 1 cut(s) 162
GsuI CTGGAG 2 cut(s) 47, 114
HaeIII GGCC 1 cut(s) 343
HapII CCGG 2 cut(s) 803, 839
HhaI GCGC 1 cut(s) 725
Hin1II CATG 5 cut(s) 169, 202, 317, 758, 864
Hin6I GCGC 1 cut(s) 723
HinP1I GCGC 1 cut(s) 723
HinfI GANTC 3 cut(s) 428, 596, 692
HpaII CCGG 2 cut(s) 803, 839
HphI GGTGA 2 cut(s) 242, 853
Hpy166II GTNNAC 2 cut(s) 151, 184
Hpy188I TCNGA 2 cut(s) 438, 496
Hpy188III TCNNGA 4 cut(s) 398, 412, 779, 901
Hpy8I GTNNAC 2 cut(s) 151, 184
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 1 cut(s) 375
HpyCH4III ACNGT 2 cut(s) 254, 879
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 6 cut(s) 347, 452, 628, 674, 713, 773
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 299, 401
HpyF3I CTNAG 2 cut(s) 38, 533
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 5 cut(s) 169, 202, 317, 758, 864
HspAI GCGC 1 cut(s) 723
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 3 cut(s) 280, 310, 566
LmnI GCTCC 3 cut(s) 91, 224, 635
Lsp1109I GCAGC 1 cut(s) 173
LweI GCATC 3 cut(s) 391, 661, 782
MaeI CTAG 1 cut(s) 779
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 3 cut(s) 21, 41, 841
MalI GATC 3 cut(s) 282, 312, 568
MboI GATC 3 cut(s) 280, 310, 566
MboII GAAGA 4 cut(s) 23, 26, 368, 662
MflI RGATCY 1 cut(s) 566
MhlI GDGCHC 1 cut(s) 640
MluCI AATT 7 cut(s) 103, 258, 457, 516, 623, 665, 797
MmeI TCCRAC 2 cut(s) 519, 542
MnlI CCTC 9 cut(s) 89, 136, 373, 475, 646, 775, 819, 822, 889
MroXI GAANNNNTTC 2 cut(s) 174, 360
MseI TTAA 4 cut(s) 122, 261, 318, 456
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 2 cut(s) 803, 839
MspR9I CCNGG 4 cut(s) 296, 489, 590, 602
MvaI CCWGG 4 cut(s) 296, 489, 590, 602
MvnI CGCG 1 cut(s) 725
MwoI GCNNNNNNNGC 3 cut(s) 170, 299, 401
NcoI CCATGG 2 cut(s) 165, 860
NdeII GATC 3 cut(s) 280, 310, 566
NlaIII CATG 5 cut(s) 169, 202, 317, 758, 864
NlaIV GGNNCC 3 cut(s) 486, 493, 752
NmuCI GTSAC 1 cut(s) 841
PdmI GAANNNNTTC 2 cut(s) 174, 360
PfeI GAWTC 3 cut(s) 428, 596, 692
PflFI GACNNNGTC 1 cut(s) 534
PfoI TCCNGGA 1 cut(s) 588
PkrI GCNGC 1 cut(s) 163
PshBI ATTAAT 1 cut(s) 261
PsiI TTATAA 1 cut(s) 515
Psp124BI GAGCTC 1 cut(s) 640
Psp6I CCWGG 4 cut(s) 294, 487, 588, 600
PspGI CCWGG 4 cut(s) 294, 487, 588, 600
PspN4I GGNNCC 3 cut(s) 486, 493, 752
PspPI GGNCC 2 cut(s) 485, 492
PstI CTGCAG 2 cut(s) 454, 715
PsuI RGATCY 1 cut(s) 566
PsyI GACNNNGTC 1 cut(s) 534
RsaI GTAC 7 cut(s) 152, 185, 275, 331, 339, 386, 767
RsaNI GTAC 7 cut(s) 151, 184, 274, 330, 338, 385, 766
SacI GAGCTC 1 cut(s) 640
SaqAI TTAA 4 cut(s) 122, 261, 318, 456
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 3 cut(s) 280, 310, 566
Sau96I GGNCC 2 cut(s) 485, 492
ScrFI CCNGG 4 cut(s) 296, 489, 590, 602
SduI GDGCHC 1 cut(s) 640
SfaNI GCATC 3 cut(s) 391, 661, 782
SfcI CTRYAG 2 cut(s) 450, 711
SinI GGWCC 2 cut(s) 485, 492
Sse9I AATT 7 cut(s) 103, 258, 457, 516, 623, 665, 797
SsiI CCGC 3 cut(s) 5, 90, 128
SspI AATATT 2 cut(s) 702, 742
SspMI CTAG 1 cut(s) 779
SstI GAGCTC 1 cut(s) 640
StyD4I CCNGG 4 cut(s) 294, 487, 588, 600
StyI CCWWGG 2 cut(s) 165, 860
TaaI ACNGT 2 cut(s) 254, 879
TaiI ACGT 1 cut(s) 111
TaqI TCGA 1 cut(s) 900
TasI AATT 7 cut(s) 103, 258, 457, 516, 623, 665, 797
TatI WGTACW 4 cut(s) 150, 183, 329, 384
TfiI GAWTC 3 cut(s) 428, 596, 692
Tru1I TTAA 4 cut(s) 122, 261, 318, 456
Tru9I TTAA 4 cut(s) 122, 261, 318, 456
TscAI CASTG 3 cut(s) 186, 226, 259
TseFI GTSAC 1 cut(s) 841
TseI GCWGC 1 cut(s) 161
Tsp45I GTSAC 1 cut(s) 841
TspDTI ATGAA 5 cut(s) 171, 279, 369, 609, 771
TspRI CASTG 3 cut(s) 186, 226, 259
Tth111I GACNNNGTC 1 cut(s) 534
VpaK11BI GGWCC 2 cut(s) 485, 492
VspI ATTAAT 1 cut(s) 261
XbaI TCTAGA 1 cut(s) 778
XmnI GAANNNNTTC 2 cut(s) 174, 360
XspI CTAG 1 cut(s) 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.