MD12G1212900.v1.1

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008545
Physical Location & Seq
Reverse (-)
29154375 .. 29158537
4163 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1212900.v1.1.491

Sequence Viewer

Length: 915 bp
ATGGCGGACGAAGAAGAAGCAGAGCTAAGCGAGAAGCAGAAGAAGGAGATAGCAAAGTGGTTCCTCCTCAACTCTCCTCCCGGCGAAATCCAATTCGTCGCCAAAGATGTGAAGGCGGTCATCAACGACGACGTTTTGTACGAGGAGGCTGCGTCGGAGGCGTTCCCACTGTACAACAAATCCCACTTGATTTCCATCGAAATGCCGGGCCGAATCGGAGACGTTATAGTTACATCATATGGTGAGCTCGCTAAGAACGAGTTCATTGATCCCAGAACGGCTCAAGTTGCTGTAGTTGACCACATCAAACAGGTTTGTACAGAGGTGAGACCTGCACTGGATGAGGAACTGCCATCTGCATATGTTGAGGAATTTCGGTGTGCTATGGATGCAGAAATACTTAAATATGTTGGTGAAGCTTATCCAAAAGGAGTCTGCTCAGTGTACTGTGGTAATGGTAAAGATGTGGAGGGCCCAGGATTTGACTTTGAGCTTGTAGTGGTGATTTCAGCTGCTAGACATAGCCCACAAAATTTCTGCAACGGTAGTTGGCGTTCAGTATGGAGCATTGAGTTCAAAGATGAAATACAAATGCTGGAATTAAAAGGCAAACTGCAGGTGGGTGCCCACTATTTCGAGGAGGGCAATGTGCAGTTAGATGCCAAGCATGAATGCAAAGATACAACAATTTTTCAGTCCTCTGAAGATAGTGCGATTGCCATTAGCAACATTATTCGTCAACATGAGACAGAGTACTTAACATCTCTTGAGGCATCTTATTCAAATTTGCCCGATACCACTTTTAAGGATCTTCGAAGAAAGCTTCCAGTTACTCGCACCCTATTTCCATGGCAGAACACCTTGCAGTTTAGCTTGACAAGAGACATTACAAAAGAACTTGGGATCGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

305

Amino Acids

34.27

Weight (kDa)

4.78

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-actin_cap_A PF01267 18 - 295 1.1e-80 F-actin capping protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 607
Acc36I ACCTGC 2 cut(s) 340, 607
AccB1I GGYRCC 1 cut(s) 623
AciI CCGC 2 cut(s) 5, 116
AclWI GGATC 3 cut(s) 263, 816, 911
AcsI RAATTY 3 cut(s) 371, 532, 784
AcuI CTGAAG 1 cut(s) 723
AfaI GTAC 5 cut(s) 140, 173, 319, 446, 755
AgsI TTSAA 2 cut(s) 577, 783
AjnI CCWGG 1 cut(s) 475
AluBI AGCT 7 cut(s) 25, 247, 419, 493, 512, 823, 873
AluI AGCT 7 cut(s) 25, 247, 419, 493, 512, 823, 873
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 4 cut(s) 213, 322, 740, 876
AlwI GGATC 3 cut(s) 263, 816, 911
AoxI GGCC 2 cut(s) 208, 472
ApaI GGGCCC 1 cut(s) 476
ApeKI GCWGC 2 cut(s) 149, 512
ApoI RAATTY 3 cut(s) 371, 532, 784
Asp700I GAANNNNTTC 1 cut(s) 260
AspS9I GGNCC 3 cut(s) 208, 472, 473
AsuC2I CCSGG 2 cut(s) 81, 207
AsuHPI GGTGA 4 cut(s) 254, 337, 425, 514
AsuII TTCGAA 1 cut(s) 814
BaeGI GKGCMC 2 cut(s) 476, 628
BanI GGYRCC 1 cut(s) 623
BanII GRGCYC 2 cut(s) 249, 476
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 2 cut(s) 136, 499
BccI CCATC 2 cut(s) 203, 361
BceAI ACGGC 1 cut(s) 294
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 1 cut(s) 477
BcnI CCSGG 2 cut(s) 81, 207
BcoDI GTCTC 4 cut(s) 213, 322, 740, 876
BfaI CTAG 1 cut(s) 516
BfmI CTRYAG 2 cut(s) 291, 614
BfuAI ACCTGC 2 cut(s) 340, 607
BisI GCNGC 2 cut(s) 150, 513
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 2 cut(s) 151, 514
BmcAI AGTACT 1 cut(s) 755
Bme1390I CCNGG 3 cut(s) 81, 207, 477
BmgT120I GGNCC 3 cut(s) 208, 472, 473
BmiI GGNNCC 3 cut(s) 62, 474, 625
BmrFI CCNGG 3 cut(s) 81, 207, 477
BmsI GCATC 3 cut(s) 379, 649, 782
Bpu1102I GCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 814
BpuEI CTTGAG 2 cut(s) 267, 788
BpuMI CCSGG 2 cut(s) 81, 207
BsaBI GATNNNNATC 1 cut(s) 194
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 2 cut(s) 475, 848
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bse1I ACTGG 2 cut(s) 342, 827
Bse3DI GCAATG 1 cut(s) 652
Bse8I GATNNNNATC 1 cut(s) 194
BseBI CCWGG 1 cut(s) 477
BseDI CCNNGG 2 cut(s) 475, 848
BseGI GGATG 2 cut(s) 346, 394
BseJI GATNNNNATC 1 cut(s) 194
BseMI GCAATG 1 cut(s) 652
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 2 cut(s) 342, 827
BseRI GAGGAG 4 cut(s) 56, 66, 158, 653
BseSI GKGCMC 2 cut(s) 476, 628
BseXI GCAGC 2 cut(s) 136, 499
BsgI GTGCAG 2 cut(s) 318, 671
BshFI GGCC 2 cut(s) 210, 474
BshNI GGYRCC 1 cut(s) 623
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 2 cut(s) 81, 206
BsmAI GTCTC 4 cut(s) 213, 322, 740, 876
BsmBI CGTCTC 1 cut(s) 213
BsmI GAATGC 1 cut(s) 677
BsnI GGCC 2 cut(s) 210, 474
Bso31I GGTCTC 1 cut(s) 322
Bsp119I TTCGAA 1 cut(s) 814
Bsp120I GGGCCC 1 cut(s) 472
Bsp1286I GDGCHC 3 cut(s) 249, 476, 628
Bsp1407I TGTACA 2 cut(s) 171, 317
Bsp143I GATC 3 cut(s) 268, 808, 903
Bsp1720I GCTNAGC 1 cut(s) 26
Bsp19I CCATGG 1 cut(s) 848
BspACI CCGC 2 cut(s) 5, 116
BspANI GGCC 2 cut(s) 210, 474
BspCNI CTCAG 1 cut(s) 452
BspLI GGNNCC 3 cut(s) 62, 474, 625
BspMAI CTGCAG 1 cut(s) 618
BspMI ACCTGC 2 cut(s) 340, 607
BspPI GGATC 3 cut(s) 263, 816, 911
BspT104I TTCGAA 1 cut(s) 814
BspT107I GGYRCC 1 cut(s) 623
BspTNI GGTCTC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 652
BsrGI TGTACA 2 cut(s) 171, 317
BsrI ACTGG 2 cut(s) 342, 827
BssECI CCNNGG 2 cut(s) 475, 848
BssMI GATC 3 cut(s) 268, 808, 903
BssT1I CCWWGG 1 cut(s) 848
Bst2UI CCWGG 1 cut(s) 477
Bst4CI ACNGT 3 cut(s) 171, 449, 545
BstAUI TGTACA 2 cut(s) 171, 317
BstBI TTCGAA 1 cut(s) 814
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 3 cut(s) 26, 252, 439
BstDSI CCRYGG 1 cut(s) 848
BstF5I GGATG 2 cut(s) 346, 394
BstKTI GATC 3 cut(s) 271, 811, 906
BstMAI GTCTC 4 cut(s) 213, 322, 740, 876
BstMBI GATC 3 cut(s) 268, 808, 903
BstMWI GCNNNNNNNGC 3 cut(s) 158, 287, 389
BstNI CCWGG 1 cut(s) 477
BstSCI CCNGG 3 cut(s) 79, 205, 475
BstSFI CTRYAG 2 cut(s) 291, 614
BstSLI GKGCMC 2 cut(s) 476, 628
BstV1I GCAGC 2 cut(s) 136, 499
BstX2I RGATCY 1 cut(s) 808
BstYI RGATCY 1 cut(s) 808
BsuRI GGCC 2 cut(s) 210, 474
BtgI CCRYGG 1 cut(s) 848
BtsCI GGATG 2 cut(s) 346, 394
BtsIMutI CAGTG 3 cut(s) 167, 335, 447
BveI ACCTGC 2 cut(s) 340, 607
Cac8I GCNNGC 1 cut(s) 249
Cfr13I GGNCC 3 cut(s) 208, 472, 473
CseI GACGC 1 cut(s) 141
Csp6I GTAC 5 cut(s) 139, 172, 318, 445, 754
CviAII CATG 3 cut(s) 668, 743, 849
CviQI GTAC 5 cut(s) 139, 172, 318, 445, 754
DdeI CTNAG 3 cut(s) 26, 252, 439
DpnI GATC 3 cut(s) 270, 810, 905
DpnII GATC 3 cut(s) 268, 808, 903
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 848
Eco24I GRGCYC 2 cut(s) 249, 476
Eco31I GGTCTC 1 cut(s) 322
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 723
EcoICRI GAGCTC 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 472
EcoRII CCWGG 1 cut(s) 475
EcoT14I CCWWGG 1 cut(s) 848
EcoT38I GRGCYC 2 cut(s) 249, 476
ErhI CCWWGG 1 cut(s) 848
Esp3I CGTCTC 1 cut(s) 213
FaeI CATG 3 cut(s) 671, 746, 852
FatI CATG 3 cut(s) 667, 742, 848
FauNDI CATATG 2 cut(s) 238, 361
Fnu4HI GCNGC 2 cut(s) 150, 513
FokI GGATG 2 cut(s) 353, 401
FriOI GRGCYC 2 cut(s) 249, 476
Fsp4HI GCNGC 2 cut(s) 150, 513
FspBI CTAG 1 cut(s) 516
GluI GCNGC 2 cut(s) 150, 513
HaeIII GGCC 2 cut(s) 210, 474
HapII CCGG 2 cut(s) 81, 206
HgaI GACGC 1 cut(s) 141
Hin1II CATG 3 cut(s) 671, 746, 852
HincII GTYRAC 2 cut(s) 298, 740
HindII GTYRAC 2 cut(s) 298, 740
HindIII AAGCTT 2 cut(s) 417, 821
HinfI GANTC 2 cut(s) 213, 432
HpaII CCGG 2 cut(s) 81, 206
HphI GGTGA 4 cut(s) 254, 337, 425, 514
Hpy166II GTNNAC 3 cut(s) 298, 445, 740
Hpy188I TCNGA 4 cut(s) 157, 218, 703, 908
Hpy188III TCNNGA 1 cut(s) 767
Hpy8I GTNNAC 3 cut(s) 298, 445, 740
Hpy99I CGWCG 4 cut(s) 101, 131, 134, 157
HpyAV CCTTC 2 cut(s) 37, 106
HpyCH4III ACNGT 3 cut(s) 171, 449, 545
HpyCH4IV ACGT 2 cut(s) 132, 222
HpyCH4V TGCA 8 cut(s) 335, 359, 392, 540, 616, 652, 675, 865
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 287, 389
HpyF3I CTNAG 3 cut(s) 26, 252, 439
HpySE526I ACGT 2 cut(s) 132, 222
Hsp92II CATG 3 cut(s) 671, 746, 852
Kzo9I GATC 3 cut(s) 268, 808, 903
LmnI GCTCC 1 cut(s) 564
Lsp1109I GCAGC 2 cut(s) 136, 499
LweI GCATC 3 cut(s) 379, 649, 782
MaeI CTAG 1 cut(s) 516
MaeII ACGT 2 cut(s) 132, 222
MaeIII GTNAC 2 cut(s) 229, 829
MalI GATC 3 cut(s) 270, 810, 905
MboI GATC 3 cut(s) 268, 808, 903
MboII GAAGA 6 cut(s) 23, 26, 52, 716, 803, 828
MflI RGATCY 1 cut(s) 808
MhlI GDGCHC 3 cut(s) 249, 476, 628
MluCI AATT 6 cut(s) 92, 371, 532, 599, 687, 784
MlyI GAGTC 1 cut(s) 441
MmeI TCCRAC 1 cut(s) 135
MroXI GAANNNNTTC 1 cut(s) 260
MseI TTAA 4 cut(s) 402, 602, 758, 804
MslI CAYNNNNRTG 1 cut(s) 200
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 2 cut(s) 81, 206
MspR9I CCNGG 3 cut(s) 81, 207, 477
Mva1269I GAATGC 1 cut(s) 677
MvaI CCWGG 1 cut(s) 477
MwoI GCNNNNNNNGC 3 cut(s) 158, 287, 389
NciI CCSGG 2 cut(s) 81, 207
NcoI CCATGG 1 cut(s) 848
NdeI CATATG 2 cut(s) 238, 361
NdeII GATC 3 cut(s) 268, 808, 903
NlaIII CATG 3 cut(s) 671, 746, 852
NlaIV GGNNCC 3 cut(s) 62, 474, 625
NspV TTCGAA 1 cut(s) 814
PaqCI CACCTGC 1 cut(s) 607
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 255
PctI GAATGC 1 cut(s) 677
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 213
PkrI GCNGC 2 cut(s) 151, 514
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 475
PspGI CCWGG 1 cut(s) 475
PspN4I GGNNCC 3 cut(s) 62, 474, 625
PspOMI GGGCCC 1 cut(s) 472
PspPI GGNCC 3 cut(s) 208, 472, 473
PsrI GAACNNNNNNTAC 2 cut(s) 538, 570
PstI CTGCAG 1 cut(s) 618
PsuI RGATCY 1 cut(s) 808
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 5 cut(s) 140, 173, 319, 446, 755
RsaNI GTAC 5 cut(s) 139, 172, 318, 445, 754
RseI CAYNNNNRTG 1 cut(s) 200
SacI GAGCTC 1 cut(s) 249
SaqAI TTAA 4 cut(s) 402, 602, 758, 804
SatI GCNGC 2 cut(s) 150, 513
Sau3AI GATC 3 cut(s) 268, 808, 903
Sau96I GGNCC 3 cut(s) 208, 472, 473
ScaI AGTACT 1 cut(s) 755
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 3 cut(s) 81, 207, 477
SduI GDGCHC 3 cut(s) 249, 476, 628
SfaNI GCATC 3 cut(s) 379, 649, 782
SfcI CTRYAG 2 cut(s) 291, 614
SfuI TTCGAA 1 cut(s) 814
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 2 cut(s) 282, 767
SmoI CTYRAG 2 cut(s) 282, 767
Sse9I AATT 6 cut(s) 92, 371, 532, 599, 687, 784
SsiI CCGC 2 cut(s) 5, 116
SspMI CTAG 1 cut(s) 516
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 3 cut(s) 79, 205, 475
StyI CCWWGG 1 cut(s) 848
TaaI ACNGT 3 cut(s) 171, 449, 545
TaiI ACGT 2 cut(s) 135, 225
TaqI TCGA 3 cut(s) 198, 636, 814
TasI AATT 6 cut(s) 92, 371, 532, 599, 687, 784
TatI WGTACW 4 cut(s) 171, 317, 444, 753
TfiI GAWTC 1 cut(s) 213
Tru1I TTAA 4 cut(s) 402, 602, 758, 804
Tru9I TTAA 4 cut(s) 402, 602, 758, 804
TscAI CASTG 3 cut(s) 174, 342, 447
TseI GCWGC 2 cut(s) 149, 512
TspDTI ATGAA 3 cut(s) 253, 597, 684
TspRI CASTG 3 cut(s) 174, 342, 447
XapI RAATTY 3 cut(s) 371, 532, 784
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 1 cut(s) 516
ZrmI AGTACT 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.