MD04G1200300.v1.1

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008545
Physical Location & Seq
Reverse (-)
28688224 .. 28691873
3650 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1200300.v1.1.491

Sequence Viewer

Length: 921 bp
ATGGCGGACGAAGAAGAAGCAGAGCTAAGTGAGAAGCAGAAGAAAGAGATAGCAAAGTGGTTCCTCCTCAACTCTCCCCCCGGAGAAATCCAATTCGTCGCTAAAGATGTGAAGGCGGTAATCAACGACGACGTTTTGTACGAGGAGGCTGCGTCGGAGGCCTTCCCACTGTACAACAAATCCCACTTGATTTCCATCGAAATGCCGGGCCGAATCGGCGACGTTATAGTTACATCATATGGTGAGGTCGGTGAGACCGAGTTCATTGATCCCAGGACAGCTCAAGTTGCTGTAGTTAACCACACTAAACAGGTTTGTACAGAGGTGAGACCTGCTCTGGATGAGGAACTTCCATCCGCATATGTTGAGGAATTCCGGTGTGCTCTGGATGCAGAAATACTTAAATATGTTGGTGAAGCTTATCCAAAAGGAGTCTGCTCAGTGTACTGTGGCAATGGTAAAGGTGTGGAGGGCCCTGGATTTGACTTTGAGCTTGTAGTGGTGATTTCAGCTGCTAGACACAGCCCACAAAATTTCTGTAACGGTAGTTGGCGTTCTGTATGGAGCATTGAGTTCAAAGATGAAATACAAATGCTGGAATTAAAAGGCAAACTGCAGGTGGGTGCCCACTATTTTGAGGAGGGCAATGTGCAGTTAGATGCAAAACATGAATGCAAGGATACAACAATTTTTCAGGTTGATTCCTCTGAAGATAGTGCAATTGCCATTAGCAACATTATTCGTCAACACGAGACTGAGTACATAACATCTCTTGAGGCATCTTATTCAAATTTGCCTGATACCACTTTCAAGGATCTTCGAAGAAAGCTTCCAGTTACGCGCACCCTGTTTCCGTGGCAGAATACCTTGCAGTTCAGCTTGACGAGAGACATCACCAAAGAACTTGGCATCGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

307

Amino Acids

34.35

Weight (kDa)

4.75

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-actin_cap_A PF01267 18 - 297 3.3e-79 F-actin capping protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 607
Acc36I ACCTGC 2 cut(s) 340, 607
AccB1I GGYRCC 1 cut(s) 623
AccII CGCG 1 cut(s) 841
AciI CCGC 3 cut(s) 5, 116, 357
AclWI GGATC 2 cut(s) 263, 822
AcsI RAATTY 3 cut(s) 371, 532, 790
AcuI CTGAAG 1 cut(s) 729
AfaI GTAC 5 cut(s) 140, 173, 319, 446, 761
AgsI TTSAA 3 cut(s) 577, 789, 811
AjnI CCWGG 2 cut(s) 272, 475
AluBI AGCT 7 cut(s) 25, 281, 419, 493, 512, 829, 879
AluI AGCT 7 cut(s) 25, 281, 419, 493, 512, 829, 879
Alw21I GWGCWC 1 cut(s) 385
Alw26I GTCTC 4 cut(s) 248, 322, 746, 882
AlwI GGATC 2 cut(s) 263, 822
AoxI GGCC 3 cut(s) 159, 208, 472
ApaI GGGCCC 1 cut(s) 476
ApeKI GCWGC 2 cut(s) 149, 512
ApoI RAATTY 3 cut(s) 371, 532, 790
AspLEI GCGC 1 cut(s) 843
AspS9I GGNCC 3 cut(s) 208, 472, 473
AsuC2I CCSGG 2 cut(s) 81, 207
AsuHPI GGTGA 6 cut(s) 254, 263, 337, 425, 514, 886
AsuII TTCGAA 1 cut(s) 820
BaeGI GKGCMC 2 cut(s) 476, 628
BanI GGYRCC 1 cut(s) 623
BanII GRGCYC 1 cut(s) 476
BauI CACGAG 1 cut(s) 749
Bbv12I GWGCWC 1 cut(s) 385
BbvI GCAGC 2 cut(s) 136, 499
BccI CCATC 2 cut(s) 203, 361
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 2 cut(s) 274, 477
BciVI GTATCC 1 cut(s) 673
BcnI CCSGG 2 cut(s) 81, 207
BcoDI GTCTC 4 cut(s) 248, 322, 746, 882
BfaI CTAG 1 cut(s) 516
BfmI CTRYAG 2 cut(s) 291, 614
BfuAI ACCTGC 2 cut(s) 340, 607
BfuI GTATCC 1 cut(s) 673
BglI GCCNNNNNGGC 1 cut(s) 216
BisI GCNGC 2 cut(s) 150, 513
BlsI GCNGC 2 cut(s) 151, 514
Bme1390I CCNGG 4 cut(s) 81, 207, 274, 477
BmgT120I GGNCC 3 cut(s) 208, 472, 473
BmiI GGNNCC 3 cut(s) 62, 474, 625
BmrFI CCNGG 4 cut(s) 81, 207, 274, 477
BmsI GCATC 3 cut(s) 379, 649, 788
BplI GAGNNNNNCTC 2 cut(s) 319, 351
Bpu14I TTCGAA 1 cut(s) 820
BpuEI CTTGAG 2 cut(s) 267, 794
BpuMI CCSGG 2 cut(s) 81, 207
BsaBI GATNNNNATC 1 cut(s) 194
BsaI GGTCTC 2 cut(s) 248, 322
BsaJI CCNNGG 4 cut(s) 79, 272, 475, 854
BsaWI WCCGGW 1 cut(s) 375
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bse1I ACTGG 1 cut(s) 833
Bse3DI GCAATG 2 cut(s) 460, 652
Bse8I GATNNNNATC 1 cut(s) 194
BseBI CCWGG 2 cut(s) 274, 477
BseDI CCNNGG 4 cut(s) 79, 272, 475, 854
BseGI GGATG 3 cut(s) 346, 353, 394
BseJI GATNNNNATC 1 cut(s) 194
BseMI GCAATG 2 cut(s) 460, 652
BseMII CTCAG 2 cut(s) 453, 747
BseNI ACTGG 1 cut(s) 833
BseRI GAGGAG 3 cut(s) 56, 158, 653
BseSI GKGCMC 2 cut(s) 476, 628
BseXI GCAGC 2 cut(s) 136, 499
BsgI GTGCAG 1 cut(s) 671
Bsh1236I CGCG 1 cut(s) 841
BshFI GGCC 3 cut(s) 161, 210, 474
BshNI GGYRCC 1 cut(s) 623
BsiHKAI GWGCWC 1 cut(s) 385
BsiSI CCGG 3 cut(s) 81, 206, 376
BsmAI GTCTC 4 cut(s) 248, 322, 746, 882
BsmI GAATGC 1 cut(s) 677
BsnI GGCC 3 cut(s) 161, 210, 474
Bso31I GGTCTC 2 cut(s) 248, 322
Bsp119I TTCGAA 1 cut(s) 820
Bsp120I GGGCCC 1 cut(s) 472
Bsp1286I GDGCHC 3 cut(s) 385, 476, 628
Bsp1407I TGTACA 2 cut(s) 171, 317
Bsp143I GATC 2 cut(s) 268, 814
BspACI CCGC 3 cut(s) 5, 116, 357
BspANI GGCC 3 cut(s) 161, 210, 474
BspCNI CTCAG 2 cut(s) 452, 748
BspFNI CGCG 1 cut(s) 841
BspLI GGNNCC 3 cut(s) 62, 474, 625
BspMAI CTGCAG 1 cut(s) 618
BspMI ACCTGC 2 cut(s) 340, 607
BspPI GGATC 2 cut(s) 263, 822
BspT104I TTCGAA 1 cut(s) 820
BspT107I GGYRCC 1 cut(s) 623
BspTNI GGTCTC 2 cut(s) 248, 322
BsrDI GCAATG 2 cut(s) 460, 652
BsrGI TGTACA 2 cut(s) 171, 317
BsrI ACTGG 1 cut(s) 833
BssECI CCNNGG 4 cut(s) 79, 272, 475, 854
BssMI GATC 2 cut(s) 268, 814
BssSI CACGAG 1 cut(s) 749
Bst2BI CACGAG 1 cut(s) 749
Bst2UI CCWGG 2 cut(s) 274, 477
Bst4CI ACNGT 3 cut(s) 171, 449, 545
BstAUI TGTACA 2 cut(s) 171, 317
BstBI TTCGAA 1 cut(s) 820
BstDEI CTNAG 3 cut(s) 26, 439, 756
BstDSI CCRYGG 1 cut(s) 854
BstF5I GGATG 3 cut(s) 346, 353, 394
BstFNI CGCG 1 cut(s) 841
BstHHI GCGC 1 cut(s) 843
BstKTI GATC 2 cut(s) 271, 817
BstMAI GTCTC 4 cut(s) 248, 322, 746, 882
BstMBI GATC 2 cut(s) 268, 814
BstMWI GCNNNNNNNGC 4 cut(s) 158, 216, 287, 389
BstNI CCWGG 2 cut(s) 274, 477
BstSCI CCNGG 4 cut(s) 79, 205, 272, 475
BstSFI CTRYAG 2 cut(s) 291, 614
BstSLI GKGCMC 2 cut(s) 476, 628
BstUI CGCG 1 cut(s) 841
BstV1I GCAGC 2 cut(s) 136, 499
BstX2I RGATCY 1 cut(s) 814
BstYI RGATCY 1 cut(s) 814
BsuI GTATCC 1 cut(s) 673
BsuRI GGCC 3 cut(s) 161, 210, 474
BtgI CCRYGG 1 cut(s) 854
BtsCI GGATG 3 cut(s) 346, 353, 394
BtsIMutI CAGTG 2 cut(s) 167, 447
BveI ACCTGC 2 cut(s) 340, 607
CfoI GCGC 1 cut(s) 843
Cfr13I GGNCC 3 cut(s) 208, 472, 473
CseI GACGC 1 cut(s) 141
Csp6I GTAC 5 cut(s) 139, 172, 318, 445, 760
CviAII CATG 1 cut(s) 668
CviQI GTAC 5 cut(s) 139, 172, 318, 445, 760
DdeI CTNAG 3 cut(s) 26, 439, 756
DpnI GATC 2 cut(s) 270, 816
DpnII GATC 2 cut(s) 268, 814
EciI GGCGGA 1 cut(s) 20
Eco147I AGGCCT 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 476
Eco31I GGTCTC 2 cut(s) 248, 322
Eco57I CTGAAG 1 cut(s) 729
EcoO109I RGGNCCY 2 cut(s) 472, 473
EcoRI GAATTC 1 cut(s) 371
EcoRII CCWGG 2 cut(s) 272, 475
EcoT38I GRGCYC 1 cut(s) 476
FaeI CATG 1 cut(s) 671
FaiI YATR 9 cut(s) 227, 238, 240, 361, 363, 408, 562, 669, 764
FatI CATG 1 cut(s) 667
FauNDI CATATG 2 cut(s) 238, 361
Fnu4HI GCNGC 2 cut(s) 150, 513
FokI GGATG 3 cut(s) 340, 353, 401
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 2 cut(s) 150, 513
FspBI CTAG 1 cut(s) 516
GlaI GCGC 1 cut(s) 842
GluI GCNGC 2 cut(s) 150, 513
HaeIII GGCC 3 cut(s) 161, 210, 474
HapII CCGG 3 cut(s) 81, 206, 376
HgaI GACGC 1 cut(s) 141
HhaI GCGC 1 cut(s) 843
Hin1II CATG 1 cut(s) 671
Hin6I GCGC 1 cut(s) 841
HinP1I GCGC 1 cut(s) 841
HincII GTYRAC 2 cut(s) 298, 746
HindII GTYRAC 2 cut(s) 298, 746
HindIII AAGCTT 2 cut(s) 417, 827
HinfI GANTC 3 cut(s) 213, 432, 701
HpaI GTTAAC 1 cut(s) 298
HpaII CCGG 3 cut(s) 81, 206, 376
HphI GGTGA 6 cut(s) 254, 263, 337, 425, 514, 886
Hpy166II GTNNAC 3 cut(s) 298, 445, 746
Hpy188I TCNGA 3 cut(s) 157, 709, 914
Hpy188III TCNNGA 3 cut(s) 338, 386, 773
Hpy8I GTNNAC 3 cut(s) 298, 445, 746
Hpy99I CGWCG 5 cut(s) 101, 131, 134, 157, 224
HpyAV CCTTC 2 cut(s) 106, 172
HpyCH4III ACNGT 3 cut(s) 171, 449, 545
HpyCH4IV ACGT 2 cut(s) 132, 222
HpyCH4V TGCA 7 cut(s) 392, 616, 652, 662, 675, 719, 871
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 216, 287, 389
HpyF3I CTNAG 3 cut(s) 26, 439, 756
HpySE526I ACGT 2 cut(s) 132, 222
Hsp92II CATG 1 cut(s) 671
HspAI GCGC 1 cut(s) 841
KspAI GTTAAC 1 cut(s) 298
Kzo9I GATC 2 cut(s) 268, 814
LmnI GCTCC 1 cut(s) 564
Lsp1109I GCAGC 2 cut(s) 136, 499
LweI GCATC 3 cut(s) 379, 649, 788
MaeI CTAG 1 cut(s) 516
MaeII ACGT 2 cut(s) 132, 222
MaeIII GTNAC 3 cut(s) 229, 539, 835
MalI GATC 2 cut(s) 270, 816
MboI GATC 2 cut(s) 268, 814
MboII GAAGA 6 cut(s) 23, 26, 52, 722, 809, 834
MfeI CAATTG 1 cut(s) 720
MflI RGATCY 1 cut(s) 814
MhlI GDGCHC 3 cut(s) 385, 476, 628
MluCI AATT 7 cut(s) 92, 371, 532, 599, 687, 720, 790
MlyI GAGTC 1 cut(s) 441
MmeI TCCRAC 1 cut(s) 135
MseI TTAA 3 cut(s) 297, 402, 602
MslI CAYNNNNRTG 1 cut(s) 200
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 3 cut(s) 81, 206, 376
MspR9I CCNGG 4 cut(s) 81, 207, 274, 477
MunI CAATTG 1 cut(s) 720
Mva1269I GAATGC 1 cut(s) 677
MvaI CCWGG 2 cut(s) 274, 477
MvnI CGCG 1 cut(s) 841
MwoI GCNNNNNNNGC 4 cut(s) 158, 216, 287, 389
NciI CCSGG 2 cut(s) 81, 207
NdeI CATATG 2 cut(s) 238, 361
NdeII GATC 2 cut(s) 268, 814
NlaIII CATG 1 cut(s) 671
NlaIV GGNNCC 3 cut(s) 62, 474, 625
NspV TTCGAA 1 cut(s) 820
PaqCI CACCTGC 1 cut(s) 607
PceI AGGCCT 1 cut(s) 161
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 255
PctI GAATGC 1 cut(s) 677
PfeI GAWTC 2 cut(s) 213, 701
PkrI GCNGC 2 cut(s) 151, 514
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
Psp6I CCWGG 2 cut(s) 272, 475
PspGI CCWGG 2 cut(s) 272, 475
PspN4I GGNNCC 3 cut(s) 62, 474, 625
PspOMI GGGCCC 1 cut(s) 472
PspPI GGNCC 3 cut(s) 208, 472, 473
PsrI GAACNNNNNNTAC 2 cut(s) 538, 570
PstI CTGCAG 1 cut(s) 618
PsuI RGATCY 1 cut(s) 814
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 5 cut(s) 140, 173, 319, 446, 761
RsaNI GTAC 5 cut(s) 139, 172, 318, 445, 760
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 3 cut(s) 297, 402, 602
SatI GCNGC 2 cut(s) 150, 513
Sau3AI GATC 2 cut(s) 268, 814
Sau96I GGNCC 3 cut(s) 208, 472, 473
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 4 cut(s) 81, 207, 274, 477
SduI GDGCHC 3 cut(s) 385, 476, 628
SfaNI GCATC 3 cut(s) 379, 649, 788
SfcI CTRYAG 2 cut(s) 291, 614
SfuI TTCGAA 1 cut(s) 820
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 2 cut(s) 282, 773
SmoI CTYRAG 2 cut(s) 282, 773
Sse9I AATT 7 cut(s) 92, 371, 532, 599, 687, 720, 790
SseBI AGGCCT 1 cut(s) 161
SsiI CCGC 3 cut(s) 5, 116, 357
SspMI CTAG 1 cut(s) 516
StuI AGGCCT 1 cut(s) 161
StyD4I CCNGG 4 cut(s) 79, 205, 272, 475
TaaI ACNGT 3 cut(s) 171, 449, 545
TaiI ACGT 2 cut(s) 135, 225
TaqI TCGA 2 cut(s) 198, 820
TaqII GACCGA 1 cut(s) 272
TasI AATT 7 cut(s) 92, 371, 532, 599, 687, 720, 790
TatI WGTACW 4 cut(s) 171, 317, 444, 759
TfiI GAWTC 2 cut(s) 213, 701
Tru1I TTAA 3 cut(s) 297, 402, 602
Tru9I TTAA 3 cut(s) 297, 402, 602
TscAI CASTG 2 cut(s) 174, 447
TseI GCWGC 2 cut(s) 149, 512
TspDTI ATGAA 3 cut(s) 253, 597, 684
TspGWI ACGGA 1 cut(s) 843
TspRI CASTG 2 cut(s) 174, 447
XapI RAATTY 3 cut(s) 371, 532, 790
XspI CTAG 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.